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1.
Int J Food Microbiol ; 316: 108425, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31715547

RESUMO

Chia, is a gluten-free, rich in proteins, oilseed that is "on trend" as an alternative ingredient in food production, adding nutritional value. As a reservoir of natural biodiversity, lactic acid bacteria development, during spontaneous chia flour fermentation (sourdough) for 10 days, were investigated by culturing and high throughput sequencing (HTS). Culture-dependent analysis showed a rapid increase in total LAB numbers from the second day of sourdough refreshment. Taxonomical identification of LAB isolates by rep-PCR and further 16S rRNA sequencing was performed. Besides Among identified LAB by culture-dependent approach, species from genus Enterococcus were the most abundant; Lactococcus (Lc. lactis), Lactobacillus (L. rhamnosus) and Weissella (W. cibaria) species were also isolated. By HTS, twelve OTUs belonging to LAB genera were identified during chia sourdough fermentation with an increased Lactobacillus diversity. Enterococcus (E.) faecium, E. mundtii, W. cibaria and L. rhamnosus were detected as dominant species in the final propagation stages while Bacillus and Clostridium were mostly present during first fermentation stages. The investigation of biotechnological and safety traits (acidification ability, protein hydrolysis, exopolysaccharides production, antimicrobial activity and antibiotic resistance) of 15 representative LAB strains was performed. Strains characterization led to the selection of Lc. lactis CH179, L. rhamnosus CH34 and W. cibaria CH28 as candidates to be used as novel functional starter culture for gluten-free chia fermented products. As far as we know, this is the first study providing information on the molecular inventory of LAB population during spontaneous fermentation of chia sourdough.

2.
J Manipulative Physiol Ther ; 42(4): 219-226, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31255310

RESUMO

OBJECTIVE: The purpose of this study was to correlate the heart rate variability (HRV) indices with variables of pain that were experienced by individuals with chronic neck pain. METHODS: This was a blinded cross-sectional study. Individuals with chronic neck pain (n = 15) and healthy participants (n = 15), both sedentary and between 18 and 45 years of age, were included. The neck pain was assessed with the Numerical Rating Scale at rest and during cervical movements, Neck Disability Index, Catastrophic Thoughts about Pain Scale, and Tampa Scale of Kinesiophobia. The HRV indices (linear and nonlinear) were used for assessment of autonomic function at rest (in supine, sitting, and standing positions). RESULTS: We observed significant correlations between the NRS, Neck Disability Index, and Catastrophic Thoughts about Pain Scale with the linear and nonlinear HRV indices (P < .05, r ≥ 0.362), so that the worst HRV indices are associated with conditions of more intense and disabling neck pain. CONCLUSION: The HRV indices were significantly associated with pain intensity, disabilty, and catastrophizing in individuals with chronic neck pain.

3.
Rev. bras. ter. intensiva ; 30(4): 479-486, out.-dez. 2018. tab, graf
Artigo em Português | LILACS-Express | ID: biblio-977993

RESUMO

RESUMO Objetivo: Avaliar a força da musculatura respiratória e periférica após cirurgia cardíaca, e comparar as modificações nestas variáveis no terceiro e no sexto dias pós-operatórios. Métodos: Recrutaram-se 46 pacientes, dos quais 29 eram homens, com média de idade de 60,50 anos (DP = 9,20). Foram submetidos à cirurgia de revascularização do miocárdio 36 pacientes, cinco pacientes foram submetidos à substituição de válvula aórtica, e outros cinco à substituição da válvula mitral. Resultados: Observaram-se redução significante da força da musculatura respiratória e periférica, e significante aumento da intensidade da dor no terceiro e no sexto dias pós-operatórios (p < 0,05), exceto para a variável pressão inspiratória máxima. No sexto dia pós-operatório, os valores da pressão inspiratória máxima já tinham nível similar aos do período pré-operatório e aos valores previstos (p > 0,05). Ocorreu associação entre a força da musculatura periférica, especificamente entre a pressão expiratória máxima no pré-operatório (rs = 0,383; p = 0,009), no terceiro dia pós-operatório (rs = 0,468; p = 0,001) e no sexto dia pós-operatório (rs = 0,311; p = 0,037). Os tamanhos de efeitos foram coerentes em nível moderado à grande para força muscular respiratória, escores segundo a escala Medical Research Council e a Escala Visual Analógica, em particular entre a avaliação pré-operatória e a do sexto dia pós-operatório. Conclusão: Após cirurgia cardíaca, ocorre diminuição da força muscular respiratória e periférica. Além disto, a pressão expiratória máxima é a variável mais associada com a força muscular periférica. Essas variáveis, especialmente a força muscular respiratória e periférica, devem ser consideradas pelos profissionais que atuam no ambiente de terapia intensiva.


ABSTRACT Objective: To evaluate respiratory and peripheral muscle strength after cardiac surgery. Additionally, we compared the changes in these variables on the third and sixth postoperative days. Methods: Forty-six patients were recruited, including 17 women and 29 men, with a mean age of 60.50 years (SD = 9.20). Myocardial revascularization surgery was performed in 36 patients, replacement of the aortic valve in 5 patients, and replacement of the mitral valve in 5 patients. Results: A significant reduction in respiratory and peripheral muscle strength and a significant increase in pain intensity were observed on the third and sixth postoperative days (p < 0.05), except for the variable maximal inspiratory pressure; on the sixth postoperative day, maximal inspiratory pressure values were already similar to the preoperative and predicted values (p > 0.05). There was an association between peripheral muscle strength, specifically between maximal expiratory pressure preoperatively (rs = 0.383; p = 0.009), on the third postoperative day (rs = 0.468; p = 0.001) and on the sixth postoperative day (rs = 0.311; p = 0.037). The effect sizes were consistently moderate-to-large for respiratory muscle strength, the Medical Research Council scale and the visual analog scale, in particular between preoperative assessment and the sixth postoperative day. Conclusion: There is a decrease in respiratory and peripheral muscle strength after cardiac surgery. In addition, maximal expiratory pressure is the variable that is most associated with peripheral muscle strength. These variables, especially respiratory and peripheral muscle strength, should be considered by professionals working in the intensive care setting.

4.
J Med Syst ; 42(12): 236, 2018 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-30327942

RESUMO

Heart rate variability (HRV) among other methods can be used to assess diabetic cardiac autonomic neuropathy by cardiac intervals were recorded. However, the amount of error depending on this measurement methodology is unclear. To evaluate the intra- and inter-rater reliability to calculate HRV indices, comparing different times and by different trained examiners in patients with type 2 diabetes mellitus (T2DM). Thirty individuals of both genders, aged between 18 and 45 years, with T2DM. The RR interval (RRi) were recorded during a 10 min period on supine position using a portable heart rate monitor (Polar® S810i model). HRV indices were calculated by the software Kubios® HRV analysis (version 2.2). Linear (Mean RRi; STD RR; Mean HR; rMSSD; RR Tri; TINN LF; HF; total power) and non-linear (SD1; SD2; DFα1; DFα2, ApEn and, SampEn) indices were calculated by two examiners with an interval of one week between them. Substantial to excellent was found for reliability of the intra-examiner, with intraclass correlation coefficient (ICC) values ranging from 0.79 to 0.99, standard error of measurement (SEM) between 0.02 and 123.49 (in percentage: 1.83 and 16.67), and minimum detectable change (MDC) between 0.07 and 342.30. Regarding the inter-examiner reliability, substantial to excellent reliability was found, with ICC values ranging from 0.73 to 0.97, SEM between 0.04 and 178.13 (in percentage: 3.26 and 24.18), and MDC between 0.11 and 493.77. The use of the portable heart rate monitor to measure HRV showed acceptable intra and inter reliability in individuals with T2DM, supporting the use of this method of evaluation in research and clinical practice.

6.
Conscientiae saúde (Impr.) ; 17(3): 281-285, set. 2018.
Artigo em Português | LILACS-Express | ID: biblio-964959

RESUMO

Introdução: O sedentarismo é um dos fatores que influenciam a saúde de pacientes submetidos à hemodiálise (HD). Objetivo: Identificar o nível de atividade física de pacientes com a doença renal crônica (DCR) submetidos à HD. Métodos: Trata-se de um estudo transversal descritivo. As seguintes variáveis foram consideradas no presente estudo: idade, gênero, tempo de HD e o nível de atividade física por meio da aplicação da versão curta do International Physical Activity Questionnaire (IPAQ). Os pacientes foram classificados como muito ativo, ativo, irregularmente ativo ou sedentário. Resultados: Independente do tempo de HD, de uma maneira geral, observou-se um nível de sedentarismo variando entre 46,9 e 62,2%. Considerando todos os tempos de HD (de 1 a > 48 meses), observou-se 55,1% de pacientes sedentários. Conclusão: A maioria dos pacientes com DCR submetidos à HD apresentam nível de atividade física abaixo do recomendado, sendo classificados como sedentários segundo o IPAQ.


Introduction: Sedentarism is one of the factors that influence the health of patients undergoing hemodialysis (HD). Objective: To identify the level of physical activity of patients with chronic kidney disease (CKD) submitted to the HD. Methods: This is a descriptive cross-sectional study. The following variables were considered in the present study: age, gender, time of HD and level of physical activity by means of applying the International Physical Activity Questionnaire (IPAQ), short version. Patients were classified as very active, active, irregularly active or sedentary. Results: Regardless of the time of HD, in general, a level of sedentarism ranging from 46.9 to 62.2% was observed. Considering all times of HD (from 1 to > 48 months), 55.1% of sedentary patients were observed. Conclusion: The majority of patients with CKD undergoing HD present a level of physical activity below the recommended, classified as sedentary according to IPAQ.

7.
Arq Bras Cardiol ; 111(1): 64-72, 2018 Jul.
Artigo em Inglês, Português | MEDLINE | ID: mdl-30110046

RESUMO

BACKGROUND: Type 2 diabetes Mellitus (T2DM) is associated with cardiac autonomic dysfunction, which is an independent predictor of mortality in chronic diseases. However, whether the coexistence of systemic arterial hypertension (HTN) with DMT2 alters cardiac autonomic modulation remains unknown. OBJECTIVE: To evaluate the influence of HTN on cardiac autonomic modulation and cardiorespiratory fitness in subjects with DMT2. METHODS: 60 patients of both genders were evaluated and allocated to two groups: DMT2 patients (n = 32; 51 ± 7.5 years old) and DMT2 + HTN patients (n = 28; 51 ± 6.9 years old). RR intervals were obtained during rest in supine position. Linear and nonlinear indices of heart rate variability (HRV) were computed using Kubios HRV software. Pulmonary gas exchange was measured breath-by-breath, using a portable telemetric system during maximal incremental exercise testing on a cycle ergometer. Statistical analysis included Shapiro-Wilk test followed by Student's t Test, Pearson correlation and linear regression. RESULTS: We found that patients in the DMT2+HTN group showed lower values of mean RR intervals (801.1 vs 871.5 ms), Shannon entropy (3 vs 3.2) and fractal dimension SD 1 (9.5 vs 14.5), when contrasted with patients in the DMT2 group. Negative correlations were found between some HRV nonlinear indices and exercise capacity indices. CONCLUSION: HTN negatively affects the cardiac autonomic function in diabetic patients, who are already prone to develop autonomic dysfunction. Strategies are need to improve cardiac autonomic functionality in this population.


Assuntos
Sistema Nervoso Autônomo/fisiopatologia , Aptidão Cardiorrespiratória/fisiologia , Diabetes Mellitus Tipo 2/fisiopatologia , Angiopatias Diabéticas/fisiopatologia , Frequência Cardíaca/fisiologia , Hipertensão/fisiopatologia , Estudos Transversais , Diabetes Mellitus Tipo 2/complicações , Angiopatias Diabéticas/etiologia , Teste de Esforço , Feminino , Humanos , Hipertensão/complicações , Masculino , Pessoa de Meia-Idade
8.
Arq. bras. cardiol ; 111(1): 64-72, July 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-950197

RESUMO

Abstract Background: Type 2 diabetes Mellitus (T2DM) is associated with cardiac autonomic dysfunction, which is an independent predictor of mortality in chronic diseases. However, whether the coexistence of systemic arterial hypertension (HTN) with DMT2 alters cardiac autonomic modulation remains unknown. Objective: To evaluate the influence of HTN on cardiac autonomic modulation and cardiorespiratory fitness in subjects with DMT2. Methods: 60 patients of both genders were evaluated and allocated to two groups: DMT2 patients (n = 32; 51 ± 7.5 years old) and DMT2 + HTN patients (n = 28; 51 ± 6.9 years old). RR intervals were obtained during rest in supine position. Linear and nonlinear indices of heart rate variability (HRV) were computed using Kubios HRV software. Pulmonary gas exchange was measured breath-by-breath, using a portable telemetric system during maximal incremental exercise testing on a cycle ergometer. Statistical analysis included Shapiro-Wilk test followed by Student's t Test, Pearson correlation and linear regression. Results: We found that patients in the DMT2+HTN group showed lower values of mean RR intervals (801.1 vs 871.5 ms), Shannon entropy (3 vs 3.2) and fractal dimension SD 1 (9.5 vs 14.5), when contrasted with patients in the DMT2 group. Negative correlations were found between some HRV nonlinear indices and exercise capacity indices. Conclusion: HTN negatively affects the cardiac autonomic function in diabetic patients, who are already prone to develop autonomic dysfunction. Strategies are need to improve cardiac autonomic functionality in this population.


Resumo Fundamento: A diabetes mellitus tipo 2 (DM2) está associada com disfunção autonômica cardíaca, que é um preditor independente de mortalidade em doenças crônicas. No entanto, ainda não se sabe se a coexistência de hipertensão arterial sistêmica (HAS) e DM2 altera a modulação cardíaca autonômica. Objetivos: O objetivo deste estudo foi avaliar a influência de HAS sobre a modulação da função autonômica cardíaca e capacidade cardiopulmonar em indivíduos com DM2. Métodos: 60 pacientes de ambos os sexos foram avaliados e alocados em dois grupos; pacientes DM2 (n = 32; 51 ± 7,5 anos) e pacientes DM2 + HAS (n = 28; 51 ± 6,9 anos). Intervalos RR foram obtidos durante repouso e em posição supina. Índices lineares e não lineares da variabilidade da frequência cardíaca (VFC) foram registrados utilizando-se o programa Kubios HRV software. A troca gasosa pulmonar foi medida a cada inspiração, utilizando-se um sistema telemétrico portátil durante o teste incremental máximo de exercício em cicloergômetro. A análise estatística incluiu o teste Shapiro-Wilk seguido do teste t de Student, a correlação de Pearson e a regressão linear. Resultados: Encontramos que pacientes do grupo DM2+HAS apresentaram valores mais baixos de intervalos RR (801,1 vs 871,5 ms), entropia de Shannon (3,0 vs 3,2) e DP1 da dimensão fractal em comparação aos pacientes do grupo DM2. Foram encontradas correlações negativas entre alguns índices não lineares da VFC e índices da capacidade do exercício. Conclusão: A HAS afeta negativamente a função autonômica cardíaca em pacientes diabéticos, os quais já são propensos a desenvolverem disfunção autonômica. Estratégias são necessárias para melhorar a função autonômica cardíaca nessa população.

9.
Conscientiae saúde (Impr.) ; 17(2): 219-229, jun. 2018.
Artigo em Português | LILACS-Express | ID: biblio-916265

RESUMO

Introdução: A lombalgia se caracteriza como uma dor de origem multifatorial que pode resultar em alterações no sistema nervoso autônomo. Estas alterações são identificadas pelo aumento da modulação simpática cardíaca levando a diminuição da variabilidade da frequência cardíaca (VFC). Objetivo: Realizar uma revisão literatura acerca do uso da VFC em pacientes com lombalgia. Métodos: A busca sistemática desse estudo foi conduzida através das bases de dados LILACS, PubMED, CINAHL e SciELO. Resultados: Foram incluídos na revisão apenas dez estudos que mostravam a relação entre a lombalgia e as respostas dos índices da VFC. Destes, sete estudos relatam melhora/relação dos índices da VFC com a lombalgia e outros estudos não mostram alteração das respostas. Conclusão: Existem poucas evidências apresentando a relação entre a lombalgia e as respostas dos índices da VFC na literatura. Além disso, os resultados se mostram inconclusivos, fato este que dificulta afirmar se há relação ou melhora dos índices da VFC após intervenção em pacientes com lombalgia.


Introduction: Low back pain has multifactorial etiology and may result in changes on autonomic nervous system, such as increasing cardiac sympathetic modulation and decreasing heart rate variability (HRV). Objective: To carry out a review about the use of HRV in patients with low back pain. Methods: The systematic search of this study was conducted through the LILACS, PubMED, CINAHL and SciELO databases. Results: Ten studies that showed the relationship between low back pain and HRV index responses were included in the review. The results show that seven studies report an improvement/relation of HRV index with low back pain and other two studies did not show altered responses. Conclusion: There are few evidence reporting the relationship between low back pain and HRV responses in the literature. Moreover, the results are inconclusive which makes it difficult to affirm that there is a relationship or improvement in the HRV index after intervention in patients with low back pain.

10.
Genome Announc ; 6(10)2018 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-29519838

RESUMO

Lactobacillus helveticus is a lactic acid bacterium widely used in cheese-making and for the production of bioactive peptides from milk proteins. Here, we describe the draft genome sequence and annotation of L. helveticus strain Lh 12 isolated from natural whey starter used in the production of Grana Padano cheese.

11.
Genome Announc ; 6(8)2018 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-29472339

RESUMO

The genome sequence of a plant growth-promoting bacterium and biocontrol agent, Azospirillum brasilense REC3, isolated from strawberry roots, is reported here. The A. brasilense REC3 total genome contains 7,229,924 bp and has a G+C content of 68.7 mol%.

12.
Rev Bras Ter Intensiva ; 30(4): 479-486, 2018.
Artigo em Português, Inglês | MEDLINE | ID: mdl-30672972

RESUMO

OBJECTIVE: To evaluate respiratory and peripheral muscle strength after cardiac surgery. Additionally, we compared the changes in these variables on the third and sixth postoperative days. METHODS: Forty-six patients were recruited, including 17 women and 29 men, with a mean age of 60.50 years (SD = 9.20). Myocardial revascularization surgery was performed in 36 patients, replacement of the aortic valve in 5 patients, and replacement of the mitral valve in 5 patients. RESULTS: A significant reduction in respiratory and peripheral muscle strength and a significant increase in pain intensity were observed on the third and sixth postoperative days (p < 0.05), except for the variable maximal inspiratory pressure; on the sixth postoperative day, maximal inspiratory pressure values were already similar to the preoperative and predicted values (p > 0.05). There was an association between peripheral muscle strength, specifically between maximal expiratory pressure preoperatively (rs = 0.383; p = 0.009), on the third postoperative day (rs = 0.468; p = 0.001) and on the sixth postoperative day (rs = 0.311; p = 0.037). The effect sizes were consistently moderate-to-large for respiratory muscle strength, the Medical Research Council scale and the visual analog scale, in particular between preoperative assessment and the sixth postoperative day. CONCLUSION: There is a decrease in respiratory and peripheral muscle strength after cardiac surgery. In addition, maximal expiratory pressure is the variable that is most associated with peripheral muscle strength. These variables, especially respiratory and peripheral muscle strength, should be considered by professionals working in the intensive care setting.


Assuntos
Implante de Prótese de Valva Cardíaca/métodos , Força Muscular/fisiologia , Revascularização Miocárdica/métodos , Dor Pós-Operatória/epidemiologia , Idoso , Feminino , Humanos , Estudos Longitudinais , Masculino , Pressões Respiratórias Máximas , Pessoa de Meia-Idade , Período Pós-Operatório , Período Pré-Operatório , Músculos Respiratórios/metabolismo
13.
Motriz (Online) ; 24(3): e0086, 2018. tab
Artigo em Inglês | LILACS | ID: biblio-976249

RESUMO

The present study investigated the behavior of glycemia in subjects with type 2 diabetes (T2DM) during 36 sessions of a combined training program. Methods: This is a single arm clinical study with 20 patients with T2DM submitted to combined exercise training (strength and aerobic exercise). The sessions occurred on alternate days, 3x/week, lasting ~ 80 minutes, totaling ~ 240 minutes/week, for 12 weeks, over a total of 36 sessions. Capillary glycemia was measured before and 10 to 15 minutes after the end of each combined exercise session. Results: There was a significant reduction (p<0.05) in glycemia after application of the combined training program in 27 (75%) of the 36 sessions. Regarding the maintenance of this reduction after about 48 hours, no significant differences were identified (p>0.05). When investigating the cumulative effect of the 36 sessions of combined training program on capillary glycemia, no significant differences were observed (p>0.05). Conclusion: Glycemia exhibits a behavior of reduction immediately after concurrent strength and aerobic training programs in patients with T2DM. However, the immediate reduction of glycemia is not maintained until 48 hours, nor is there a cumulative effect of the 36 training sessions on baseline glycemia.(AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Glicemia/análise , Diabetes Mellitus/terapia , Exercícios em Circuitos , Automonitorização da Glicemia
14.
Conscientiae saúde (Impr.) ; 16(4): 441-446, dez. 2017.
Artigo em Português | LILACS-Express | ID: biblio-881745

RESUMO

Introdução: Cardiopatias congênitas estão entre as anormalidades mais comuns ao nascimento. Objetivo: Determinar as complicações pulmonares mais recorrentes no pós-operatório de cirurgia cardiopediátrica e os possíveis fatores associados a essas complicações. Metodologia: Trata-se de um estudo observacional, retrospectivo, de amostra não probabilística, realizado no período de maio de 2016 a maio de 2017, por meio da análise dos prontuários de crianças submetidas à cirurgia cardíaca no Hospital do Coração de Alagoas. Resultados: As complicações pulmonares encontradas foram atelectasia (7,3%), congestão pulmonar (7,3%) e derrame pleural (4,9%), correspondendo a 19,5% do total da amostra. A única diferença estatística encontrada foi o aumento no tempo de internação hospitalar nos indivíduos com complicações pulmonares. Conclusão: Houve predomínio de atelectasia, congestão pulmonar e derrame pleural como complicações pulmonares após cirurgia cardiopediátrica. Foi evidenciado aumento no tempo de internamento hospitalar mediante a presença de complicação pulmonar, não sendo verificada associação com outra variável aqui testada.


Introduction: Congenital heart diseases are among the most common abnormalities at birth. Objective: To determine the most recurrent pulmonary complications in the postoperative period and the possible factors associated with these complications. Methodology: This is an observational, retrospective study of a non-probabilistic sample, performed from May 2016 to May 2017. An analysis of the charts of children submitted to cardiac surgery at the Hospital of the Heart of Alagoas was performed. Results: Pulmonary complications were atelectasis (7.3%), pulmonary congestion (7.3%) and pleural effusion (4.9%), corresponding to 19.5% of the total sample. The only statistical difference found was the increase in the length of hospital stay in individuals with pulmonary complications. Conclusion: Predominated atelectasis, pulmonary and pleural effusion and pulmonary complications after pediatric cardiac surgery. It was evidenced an increase in hospital stay time due to the presence of pulmonary complications, and no association with another variable was tested.

15.
Am J Phys Med Rehabil ; 96(4): 226-235, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27386813

RESUMO

OBJECTIVE: The aim of this work was to evaluate the hemodynamic, autonomic, and metabolic responses during resistance and dynamic exercise before and after an 8-week resistance training program using a low-intensity (30% of 1 repetitium maximum), high-repetition (3 sets of 20 repetitions) model, added to an aerobic training program, in a coronary artery disease cohort. DESIGN: Twenty male subjects with coronary artery disease (61.1 ± 4.7 years) were randomly assigned to a combined training group (resistance + aerobic) or aerobic training group (AG). Heart rate, stroke volume, cardiac output, minute ventilation, blood lactate, and parasympathetic modulation indices of heart rate (square root of the mean squared differences of successive RR intervals [RMSSD] and dispersion of points perpendicular to the line of identity that provides information about the instantaneous beat-to-beat variability [SD1]) were obtained before and after an 8-week RT program while performing exercise on a cycle ergometer and a 45-degree leg press. RESULTS: Resistance training resulted in an increase in maximal and submaximal load tolerance (P < 0.01), a decreased hemodynamic response (P < 0.01), and a reduction in blood lactate in the combined training group compared to the aerobic training group during the 45-degree leg press. During exercise on a cycle ergometer, there was a decreased hemodynamic response and increased minute ventilation (P < 0.01). The 8-week RT program resulted in greater parasympathetic tone (RMSSD and SD1) and an increase in the SDNN index during exercise on a cycle ergometer and 45-degree leg press (P < 0.05). CONCLUSIONS: An 8-week resistance training program associated with aerobic training may attenuate hemodynamic stress, and modify metabolic and autonomic responses during resistance exercise. The training program also appeared to elicit beneficial cardiovascular and autonomic effects during exercise.


Assuntos
Doença da Artéria Coronariana/reabilitação , Terapia por Exercício , Treinamento de Resistência , Débito Cardíaco/fisiologia , Doença da Artéria Coronariana/fisiopatologia , Frequência Cardíaca/fisiologia , Hemodinâmica , Humanos , Ácido Láctico/sangue , Masculino , Pessoa de Meia-Idade , Ventilação Pulmonar/fisiologia , Volume Sistólico/fisiologia
16.
Bioresour Technol ; 218: 980-90, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27450128

RESUMO

The objective of this study was to investigate the effect of different animal feedings operated in two distinct PDO (protected designation of origin) cheese production areas (Parmigiano Reggiano and Grana Padano) on the microbiome of six full-scale biogas plants, by means of Illumina sequencing and qPCR techniques. The effects of feedstock (cattle slurry manure, energy crops, agro-industrial by-products), temperature (mesophilic/thermophilic), and operating time were also examined, as were the relationships between the predominant bacterial and archaeal taxa and process parameters. The different feedstocks and temperatures strongly affected the microbiomes. A more biodiverse archaeal population was highlighted in Parmigiano Reggiano area plants, suggesting an influence of the different animal feedings. Methanosarcina and Methanosaeta showed an opposite distribution among anaerobic plants, with the former found to be related to ammonium concentration. The Methanoculleus genus was more abundant in the thermophilic digester whereas representation of the Thermotogales order correlated with hydraulic retention time.


Assuntos
Biocombustíveis/microbiologia , Reatores Biológicos/microbiologia , Ração Animal , Animais , Bovinos , Queijo , Esterco , Temperatura Ambiente
17.
Int J Food Microbiol ; 236: 17-25, 2016 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-27434177

RESUMO

Llama represents for the Andean regions a valid alternative to bovine and pork meat and thanks to the high proteins and low fat content; it can constitute a good product for the novel food market. In this study, culture-dependent and independent methods were applied to investigate the microbial ecology of naturally fermented llama sausages produced in Northwest Argentina. Two different production technologies of llama sausage were investigated: a pilot-plant scale (P) and an artisanal one (A). Results obtained by High-Throughput Sequencing (HTS) of 16S rRNA amplicons showed that the production technologies influenced the development of microbial communities with a different composition throughout the entire fermentation process. Both sequencing and microbiological counts demonstrated that Lactic Acid Bacteria (LAB) contributed largely to the dominant microbiota. When a total of 230 isolates were approached by RAPD-PCR, presumptive LAB strains from P production exhibited an initial variability in RAPD fingerprints switching to a single profile at the final of ripening, while A production revealed a more heterogeneous RAPD pattern during the whole fermentation process. The constant presence of Lactobacillus sakei along the fermentation in both productions was revealed by HTS and confirmed by species-specific PCR from isolated strains. The technological characterization of Lb. sakei isolates evidenced their ability to grow at 15°C, pH4.5 and 5% NaCl (95%). Most strains hydrolyzed myofibrillar and sarcoplasmic proteins. Bacteriocins encoding genes and antimicrobial resistance were found in 35% and 42.5% of the strains, respectively. An appropriate choice of a combination of autochthonous strains in a starter formulation is fundamental to improve and standardize llama sausages safety and quality.


Assuntos
Microbiologia de Alimentos , Lactobacillus/fisiologia , Produtos da Carne/microbiologia , Animais , Argentina , Reatores Biológicos , Camelídeos Americanos , Fermentação , Lactobacillus/classificação , Lactobacillus/genética , Reação em Cadeia da Polimerase , RNA Ribossômico 16S , Técnica de Amplificação ao Acaso de DNA Polimórfico , Paladar
18.
Am J Cardiovasc Dis ; 6(2): 36-45, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27335689

RESUMO

Resistance (RE) and aerobic exercise (AE) can promote hemodynamic, physiologic and clinical modifications in coronary artery disease (CAD) patients. The aim of the study is to assess key physiologic and clinical responses during RE at 30% and 60% of 1-RM on a 45° leg press and to compare responses during AE. We evaluated fifteen male subjects with coronary artery disease (60.8±4.7 years) that performed the following tests: (1) incremental AE test on cycle ergometer; (2) 1-RM test on a leg press at 45°; (3) and RE at 30% and 60% of 1-RM for 24 repetitions. Peak cardiac output (CO), heart rate (HR), oxygen consumption (VO2), carbon dioxide production (VCO2) and the minute ventilation (VE, L/min)/VCO2 ration were measured. We found that both AE and RE at 60% of aerobic and resistance capacity elicited similar hemodynamic and ventilatory responses (p>0.05). However, RE at 30% 1-RM showed more attenuated responses of VO2, VE/VCO2, HR and CO when compared with 60% of aerobic and resistance capacity. Interestingly, the number, percentage and the severity of arrhythmias were higher at 60% 1-RM (P<0.05). Our data suggest that high repetition sets of RE at 60% 1-RM appears to result in hemodynamic, ventilatory, and metabolic changes equivalent to those observed during AE at a comparable intensity.

19.
Genome Announc ; 4(3)2016 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-27151805

RESUMO

Lactobacillus casei is used as a starter, adjunct, and/or probiotic culture in the production of fermented and functional foods. Here, we report the draft genome sequence of the respiration-competent strain L. casei N87, isolated from infant feces. This genome information may be useful for the study of respiratory metabolism in lactic acid bacteria.

20.
Genome Announc ; 4(2)2016 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-27081128

RESUMO

Clostridium sporogenesis a causative agent of food spoilage and is often used as the nontoxigenic surrogate forClostridium botulinum Here, we described the draft genome sequence and annotation ofC. sporogenesstrain UC9000 isolated from raw milk.

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