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2.
Artigo em Inglês | MEDLINE | ID: mdl-34083156

RESUMO

OBJECTIVE: Describe the clinical and laboratory characteristics and the transfusion strategy of patients at Hospital Israelita Albert Einstein with platelet refractoriness and identify their etiological characteristics. Standardize the platelet immunofluorescence technique by flow cytometry as a test for platelet compatibility in immune platelet refractoriness in transfusion support. METHODS: Review of medical records of refractory platelet patients followed at HIAE from January 2011 to May 2017. Clinical-demographic data, laboratory data and identification of the use of compatible genotyped platelets for patients in need of transfusion therapy were collected. The analyzed patients were classified according to the etiology of their platelet refractoriness. To standardize the FC-PIFT technique, blood group O platelets were incubated with serum from blood group AB donors and anti-IgG monoclonal antibody to determine the negative control. In order to verify the influence of the ABO system, monoclonal anti-IgG antibodies were incubated with blood group A or B platelets and with blood group O donor serum with isohemagglutinins below and above 1/64. RESULTS: A total of 47 patients were evaluated, a 51% (24/47) preponderance of associated immune and non-immune factors (NIPR + IPR). The most common causes of NIPR + IPR were splenomegaly (54%) and the development of HLA antibodies (88%), consistent with the literature. For patients who required therapeutic transfusion, only a small portion received compatible genotyped platelets. CONCLUSION: Although 60% of patients could benefit from the therapeutic transfusion of genotyped platelets, only 10% were actually transfused with this type of blood component. This reaffirms the need for investments in a bank of genotyped platelet donors.

3.
Harmful Algae ; 103: 102004, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33980444

RESUMO

Raphidiopsis raciborskii (formerly Cylindrospermopsis raciborskii) is a freshwater cyanobacterium potentially producing saxitoxins (STX) and cylindrospermopsin. Its ecophysiological versatility enables it to form blooms in the most diverse types of environments, from tropical to temperate, and from relatively pristine to polluted. In Peri Lake, located in the subtropical south of Brazil, growing populations of STX-producing R. raciborskii have been detected since 1994, posing risks to the use of its waters that supply a population of about 100,000 inhabitants. Despite the existence of a monitoring system for the presence and toxicity of cyanobacteria in Peri Lake water, no assessment has been made in the coastal region, downstream of outflowing lake water, thereby potentially making available a toxic biomass to natural and cultivated shellfish populations in the salt water ecosystem. To address this problem, the present study evaluated environmental variables and STX concentration by profiling the outflowing waters between Peri Lake and the adjacent coastal zone. Laboratory experiments were carried out with three strains of R. raciborskii in order to confirm the effect of salinity on STX production and verify if Perna Perna mussels fed with R. raciborskii cultures would absorb and accumulate STX. Results showed that environmental concentrations of STX reach high levels (up to 6.31 µg L-1 STX eq.), especially in the warmer months, reaching the coastal zone. In laboratory tests, it was found that the strains tolerate salinities between 4 and 6 and that salinity influences the production of STX. In addition, mussels fed with R. raciborskii effectively absorb and accumulate STX, even in typically marine salinities (22 to 30), suggesting that R. raciborskii biomass remains available and toxic despite salinity shock. These results draw attention to the ecological and health risk associated with R. raciborskii blooms, both in the lake environment and in the adjacent marine environment, calling attention to the need to improve the monitoring and management systems for water and shellfish toxicity in the region of interest, as well as other places where toxic cyanobacteria of limnic origin can reach the coastal zone.


Assuntos
Bivalves , Cianobactérias , Animais , Brasil , Cylindrospermopsis , Ecossistema , Saxitoxina
4.
Recent Pat Biotechnol ; 15(2): 89-101, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-33726659

RESUMO

BACKGROUND: Medicinal plants present activities against neurodegenerative diseases with potential for the pharmaceutical industries. Therefore, the objective of this study was to investigate the current panorama of patents and articles of Brazilian medicinal plants with pharmacological activities in the Central Nervous System (CNS), regarding such aspects as the number of patents by countries, areas of knowledge, and technological maturity. METHODS: We carry out a technological exploration on the Questel Orbit® platform with the descriptors: Agave sisalana P., Amburana cearenses A., Dimorphandra mollis B., Jatropha curcas L., Poincianella pyramidalis T. and Prosopis juliflora (Sw.) DC. with pharmacological activity and scientific exploration in PubMed and Science Direct associated with the CNS in the title, abstract, and methodology. RESULTS: A total of 642 patents were identified between the years 1999-2019. India, China, and Brazil are highlighted, 6th place, out of a total of 48 countries. Of these, 30 patents were not in the National Institute of Industrial Property, and 10% are Brazilian in biotechnology and pharmaceutical products. Eleven articles were used in PubMed and Science Direct with scientific domains (anticancer, neuroprotection and anti-inflammatory). The Federal University of Bahia is highlighted, showing Technology Readiness Levels (TRL4), basic skills of pre-clinical research. CONCLUSION: Brazilian public universities have a significant role in the scientific, technological and innovative development of therapeutic assets for CNS.


Assuntos
Plantas Medicinais , Anti-Inflamatórios , Biotecnologia , Sistema Nervoso Central , Patentes como Assunto
7.
J Ethnopharmacol ; 264: 113201, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-32814081

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Jatropha curcas L. (Euphorbiaceae), a medicinal plant known in Brazil as "Pinhão Manso", is highly adaptable, being cultivated in different tropical and subtropical regions of the world. Antimicrobial, antioxidant and antiinflammatory activities have been attributed to different parts of the plant. In the central nervous sytem (CNS), neuroinflammation is mediated by glial cells, mainly by astrocytes and microglia, a process that plays an important role in neurodegenerative diseases and other CNS disorders. In this study, we investigated the anti-inflammatory activity of the methanolic extract obtained from the leaves of J. curcas L. (MEJc) in primary cultures of glial cells submited to inflammatory stimulus. MATERIALS AND METHODS: Primary cultures of glial cells obtained from the cerebral cortex of neonate Wistar rats were treated with MEJc (0.1-50,000 µg mL-1) and its fractions (FnJc) (0.1 µg mL-1) with or without lipopolysaccharide of Escherichia coli (LPS) (1 µg mL-1). Cell viability was determined with MTT test. Modifications in glial cell morphology were investigated by means of phase contrast microscopy and May-Grünwald staining. The reactivity of astrocytes and microglia were investigated with immunocytochemistry for GFAP, Iba1 and transcription factor NF-kB, as well as with Greiss reaction to determine the nitric oxide (NO) production. RESULTS: MEJc at 0.1-1000 µg mL-1 was non-toxic to glial cells and the DE50 was 10.794 µg mL-1. The treatment with LPS induced the activation of astrocytes and microglia marked by morphological modifications and changes in the expression of GFAP and Iba1, as well as the increase in NF-kB expression and NO production. Treatment with MEJc inhibited the morphological modifications, changes in GFAP and Iba1 expression, and the increase in NF-kB and NO production induced by LPS. CONCLUSION: This study demonstrates that the MEJc and its fractions modulate inflammatory response of astrocytes and microglia to LPS and may be considered as a potential therapeutic strategy for neuroinflammation-related diseases.


Assuntos
Anti-Inflamatórios/farmacologia , Encéfalo/citologia , Encéfalo/efeitos dos fármacos , Jatropha , Neuroglia/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Anti-Inflamatórios/isolamento & purificação , Encéfalo/fisiologia , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Células Cultivadas , Neuroglia/fisiologia , Extratos Vegetais/isolamento & purificação , Ratos , Ratos Wistar
8.
Rev Bras Med Trab ; 18(1): 2-10, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32782998

RESUMO

BACKGROUND: Analyzing, understanding and managing fatigue risk in aviation is relevant for flight safety and to reduce human error. OBJECTIVE: To analyze probable levels of fatigue among a convenience sample of Brazilian civil aviation pilots and flight attendants and to develop appropriate methods and indicators to quantify potential fatigue risk in critical phases of flight (landings and takeoffs). METHODS: Data were obtained from flight rosters voluntarily and anonymously fed to a digital platform. Rosters were analyzed with software SAFTE-FAST, which considers homeostatic process and circadian cycles related to attention and wakefulness and sleep inertia. RESULTS: The rosters for January (n=248), May (n=259) and July (n=261) 2018 were associated with incidence of 77, 54 and 77% respectively of least one event of minimal effectiveness (<77%) during critical phases of flight. The distribution of minimal effectiveness and hazard area during critical phases of flight exhibited significant seasonal oscillation upon comparing the results for January and July relative to May 2018 (p<0.001). CONCLUSION: Relative likelihood of fatigue was high in the crew rosters, with significant seasonal oscillation of minimal effectiveness and hazard area in critical phases of flight. These results point to the need for improved roster management since prescriptive rules were insufficient to mitigate risk.


INTRODUÇÃO: Métodos de análise, compreensão e gerenciamento do risco da fadiga na aviação representam tópicos de interesse para a segurança de voo e mitigação de falhas humanas. OBJETIVO: Avaliar o provável nível de fadiga em uma amostra de conveniência de pilotos e comissários de voo da aviação regular brasileira, propondo metodologia e indicadores apropriados para a quantificação da potencial exposição ao risco da fadiga durante as fases críticas de voo (pousos e decolagens). MÉTODOS: Os dados foram obtidos por envio espontâneo e anônimo das escalas de voo para uma plataforma, sendo estas analisadas com o software Sleep, Activity, Fatigue, and Task Effectiveness / Fatigue Avoidance Scheduling Tool (SAFTE-FAST), que leva em conta o processo homeostático, os ritmos circadianos associados à atenção e vigília e a inércia do sono. RESULTADOS: As escalas dos meses de janeiro (n=248), maio (n=259) e julho (n=261) de 2018 tiveram incidência de 77, 54 e 77% de ao menos um evento com efetividade mínima nas fases críticas abaixo de 77%, respectivamente. As distribuições de efetividades mínimas e áreas de risco nas fases críticas apresentaram oscilação sazonal significativa, comparando os meses de janeiro ou julho com maio de 2018 (p<0,001). CONCLUSÕES: O estudo apontou probabilidade relativa elevada de fadiga nas escalas dos aeronautas, assim como oscilações sazonais significativas nas distribuições de efetividade mínima e aérea de risco nas fases críticas. Esses resultados indicam a necessidade de um melhor gerenciamento das escalas, visto que os limites prescritivos vigentes à época não foram suficientes para a mitigação dos riscos.

9.
Sci Total Environ ; 729: 138745, 2020 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-32498159

RESUMO

Marine climate change mitigation initiatives have recently attracted a great deal of interest in the role of natural carbon sinks, particularly on coastal systems. Brown seaweeds of the genus Sargassum are the largest canopy-forming algae in tropical and subtropical environments, with a wide global distribution on rocky reefs and as floating stands. Because these algae present high amounts of biomass, we suggest their contribution is relevant for global carbon stocks and consequently for mitigating climate change as CO2 remover. We modelled global distributions and quantified carbon stocks as above-ground biomass (AGB) with machine learning algorithms and climate data. Sargassum AGB totaled 13.1 Pg C at the global scale, which is a significant amount of carbon, comparable to other key marine ecosystems, such as mangrove forests, salt marshes and seagrass meadows. However, specific techniques related to bloom production and management, or the utilization of biomass for biomaterials, should be fostered.


Assuntos
Mudança Climática , Sargassum , Biomassa , Carbono , Sequestro de Carbono , Florestas , Árvores , Clima Tropical
10.
Sensors (Basel) ; 20(12)2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32586005

RESUMO

This paper describes a magnetoelastic strain sensor based on the ∆E effect and discusses some materials used in its construction. A polycrystalline Fe-Al-B alloy with good quality magnetoelastic properties was used as the transducer and glued to the test object, either brass plates or rods of SAE 1010 steel. The strain-dependent magnetic field of the transducer changes the operating point of the resonator, a strip of field-annealed Metglas 2826MB3, resulting in a modification of its resonant frequency. A model was developed to simulate the strain-dependent magnetic field acting on the resonator and thus to calculate curves of resonant frequency vs. deformation. With the help of this model, differences in the shape of the frequency vs. strain curve can be understood. For a sensor with resonant frequency of 60.5 kHz glued to a rod of SAE 1010 steel, a total resonant frequency variation ∆f ~7 kHz was observed for a deformation of 1100 ppm. The geometry of this sensor is especially favorable for the remote monitoring of a steel surface, such as the wires of the tensile armor of a marine riser.

11.
Transfus Med ; 30(4): 317-323, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32484285

RESUMO

BACKGROUND: One of the strategies used to reduce the risk of haemolysis due to ABO-minor incompatible platelet transfusions is to perform a screening test to identify group O donors with high titres of anti-A and anti-B. However, critical immunoglobulin M/ immunoglobulin G (IgM/IgG) titres remain unclear. OBJECTIVE: This study aimed to determine IgM titres of anti-A and anti-B in individual donor serum vs platelet products plasma and identify a possible association between IgM/IgG titres, haemolysin test and IgG subclasses in Brazilian blood donors from group O. METHODS: IgM anti-A and Anti-B titration tests were performed on single-donor serum and platelet product plasma by gel agglutination (GA) at room temperature. For IgG anti-A and anti-B titration, serum was first treated with 0.01 M dithiothreitol (DTT), and the test was performed by GA with incubation at 37°C. Dilution of 1:64 as the cut-off was considered for both IgM/IgG. The qualitative haemolysin test was performed in tube, adding AB fresh serum, with incubation at 37°C. IgG subclasses were determined by GA using specific monoclonal antibodies. RESULTS: An association between anti-A and anti-B IgM titres and haemolysin were demonstrated (P < .001). IgM titres in plasma samples from platelet components correlated to those in single-serum samples. IgG1/IgG3 subclasses were associated with total haemolysis and titres above 64, whereas IgG2/IgG4 subclasses were associated with the absence of haemolysis and titres below 64 (P < .001). CONCLUSION: Our data suggest that a value of 64 as a critical titre can be used as a screening test of anti-A and anti-B IgM to prevent transfusion reactions. This can be a safe and cost-effective approach for managing ABO-incompatible platelet transfusions.


Assuntos
Sistema ABO de Grupos Sanguíneos/sangue , Proteínas Hemolisinas/sangue , Hemólise , Imunoglobulina G/sangue , Isoanticorpos/sangue , Reação Transfusional , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Transfusão de Plaquetas , Reação Transfusional/sangue , Reação Transfusional/prevenção & controle
12.
Rev. bras. med. trab ; 18(1): 2-10, jan-mar.2020.
Artigo em Português | LILACS-Express | LILACS | ID: biblio-1116041

RESUMO

Introdução: Métodos de análise, compreensão e gerenciamento do risco da fadiga na aviação representam tópicos de interesse para a segurança de voo e mitigação de falhas humanas. Objetivo: Avaliar o provável nível de fadiga em uma amostra de conveniência de pilotos e comissários de voo da aviação regular brasileira, propondo metodologia e indicadores apropriados para a quantificação da potencial exposição ao risco da fadiga durante as fases críticas de voo (pousos e decolagens). Métodos: Os dados foram obtidos por envio espontâneo e anônimo das escalas de voo para uma plataforma, sendo estas analisadas com o software Sleep, Activity, Fatigue, and Task Effectiveness / Fatigue Avoidance Scheduling Tool (SAFTE-FAST), que leva em conta o processo homeostático, os ritmos circadianos associados à atenção e vigília e a inércia do sono. Resultados: As escalas dos meses de janeiro (n=248), maio (n=259) e julho (n=261) de 2018 tiveram incidência de 77, 54 e 77% de ao menos um evento com efetividade mínima nas fases críticas abaixo de 77%, respectivamente. As distribuições de efetividades mínimas e áreas de risco nas fases críticas apresentaram oscilação sazonal significativa, comparando os meses de janeiro ou julho com maio de 2018 (p<0,001). Conclusões: O estudo apontou probabilidade relativa elevada de fadiga nas escalas dos aeronautas, assim como oscilações sazonais significativas nas distribuições de efetividade mínima e aérea de risco nas fases críticas. Esses resultados indicam a necessidade de um melhor gerenciamento das escalas, visto que os limites prescritivos vigentes à época não foram suficientes para a mitigação dos riscos.


Background: Analyzing, understanding and managing fatigue risk in aviation is relevant for flight safety and to reduce human error. Objective: To analyze probable levels of fatigue among a convenience sample of Brazilian civil aviation pilots and flight attendants and to develop appropriate methods and indicators to quantify potential fatigue risk in critical phases of flight (landings and takeoffs). Methods: Data were obtained from flight rosters voluntarily and anonymously fed to a digital platform. Rosters were analyzed with software SAFTEFAST, which considers homeostatic process and circadian cycles related to attention and wakefulness and sleep inertia. Results: The rosters for January (n=248), May (n=259) and July (n=261) 2018 were associated with incidence of 77, 54 and 77% respectively of least one event of minimal effectiveness (<77%) during critical phases of flight. The distribution of minimal effectiveness and hazard area during critical phases of flight exhibited significant seasonal oscillation upon comparing the results for January and July relative to May 2018 (p<0.001). Conclusion: Relative likelihood of fatigue was high in the crew rosters, with significant seasonal oscillation of minimal effectiveness and hazard area in critical phases of flight. These results point to the need for improved roster management since prescriptive rules were insufficient to mitigate risk.

13.
Einstein (Sao Paulo) ; 18: eRC4582, 2020.
Artigo em Inglês, Português | MEDLINE | ID: mdl-31531557

RESUMO

The correct identification of erythrocyte antibodies is fundamental for the searching for compatible blood and haemolytic transfusion reactions prevention. Antibodies against antigens of high prevalence are difficult to identify because of the rarity of their occurrence and unavailability of negative red cells for confirmation. We report a case of 46-years-old woman, diagnosed with hemoglobinopathy, and who had symptomatic fall in hemoglobin levels (5.3g/dL) after blood transfusion suggestive of transfusion reaction. The patient's blood type was O RhD-positive. Irregular antibody screening was positive and demonstrated a panreaction against all erythrocytes tested, but this result was not reactive with dithiothreitol. Using negative red cells for antigens of high prevalence of our inventory we could identify in the serum of the same erythrocytes an anti-Holley antibody associated with anti-E. Molecular analysis confirmed that the patient was negative for E and Holley antigens. The crossmath with compatible units confirmed the results. Holley is a high prevalence antigen of the Dombrock blood system whose negative phenotype is extremely rare in all populations and is associated with hemolytic transfusion reactions. This is an antibody that is difficult to identify because laboratories need to have experience in solving complex cases, and have available a large stock of rare sera and erythrocytes, as well other tools such as enzymes, thiol reagents and molecular tests. The correct identification of a rare antibody is initial and mandatory for searching of compatible donors, and to guarantee a satisfactory transfusional support.


Assuntos
Anticorpos/imunologia , Antígenos de Grupos Sanguíneos/imunologia , Incompatibilidade de Grupos Sanguíneos/imunologia , Reação Transfusional/imunologia , Anticorpos/sangue , Eritrócitos/imunologia , Feminino , Testes Hematológicos/métodos , Humanos , Imunoglobulinas/sangue , Isoanticorpos/imunologia , Pessoa de Meia-Idade
14.
Einstein (Säo Paulo) ; 18: eRC4582, 2020.
Artigo em Inglês | LILACS | ID: biblio-1039732

RESUMO

ABSTRACT The correct identification of erythrocyte antibodies is fundamental for the searching for compatible blood and haemolytic transfusion reactions prevention. Antibodies against antigens of high prevalence are difficult to identify because of the rarity of their occurrence and unavailability of negative red cells for confirmation. We report a case of 46-years-old woman, diagnosed with hemoglobinopathy, and who had symptomatic fall in hemoglobin levels (5.3g/dL) after blood transfusion suggestive of transfusion reaction. The patient's blood type was O RhD-positive. Irregular antibody screening was positive and demonstrated a panreaction against all erythrocytes tested, but this result was not reactive with dithiothreitol. Using negative red cells for antigens of high prevalence of our inventory we could identify in the serum of the same erythrocytes an anti-Holley antibody associated with anti-E. Molecular analysis confirmed that the patient was negative for E and Holley antigens. The crossmath with compatible units confirmed the results. Holley is a high prevalence antigen of the Dombrock blood system whose negative phenotype is extremely rare in all populations and is associated with hemolytic transfusion reactions. This is an antibody that is difficult to identify because laboratories need to have experience in solving complex cases, and have available a large stock of rare sera and erythrocytes, as well other tools such as enzymes, thiol reagents and molecular tests. The correct identification of a rare antibody is initial and mandatory for searching of compatible donors, and to guarantee a satisfactory transfusional support.


RESUMO A correta identificação dos anticorpos eritrocitários é fundamental na busca de sangue compatível e na prevenção das reações transfusionais hemolíticas. Anticorpos contra antígenos de alta prevalência são de difícil identificação, devido à raridade de sua ocorrência e à indisponibilidade de hemácias negativas para sua confirmação. Apresentamos aqui o caso de uma paciente do sexo feminino, 46 anos, com diagnóstico de hemoglobinopatia, que apresentou queda sintomática dos níveis de hemoglobina (5,3g/dL) após transfusão sanguínea, sugestiva de reação transfusional. O tipo sanguíneo da paciente era O RhD-positivo. A pesquisa de anticorpos irregulares foi positiva, demonstrando panreação contra todos os eritrócitos testados, mas não reativo ao ditiotreitol. Utilizando hemácias selecionadas negativas para antígenos de alta prevalência do nosso inventário, foi possível identificar no soro da mesma um anticorpo anti-Holley associado a um anti-E. A análise molecular confirmou que a paciente era negativa para os antígenos E e Holley, e as provas de compatibilidade com unidades fenotipadas confirmaram os resultados. Holley é um antígeno de alta prevalência do sistema sanguíneo Dombrock, cujo fenótipo negativo é extremamente raro em todas as populações e está associado a reações transfusionais hemolíticas. Trata-se de anticorpo de difícil identificação, pois os laboratórios precisam ter experiência na resolução de casos complexos, grande estoque de soros e eritrócitos raros, além de outras ferramentas, como enzimas, reagentes tiol e testes moleculares. A identificação correta de um anticorpo raro é inicial e obrigatória para a busca de doadores compatíveis, garantindo um suporte transfusional satisfatório.


Assuntos
Humanos , Feminino , Incompatibilidade de Grupos Sanguíneos/imunologia , Antígenos de Grupos Sanguíneos/imunologia , Reação Transfusional/imunologia , Anticorpos/imunologia , Imunoglobulinas/sangue , Eritrócitos/imunologia , Testes Hematológicos/métodos , Isoanticorpos/imunologia , Pessoa de Meia-Idade , Anticorpos/sangue
15.
Ecotoxicol Environ Saf ; 174: 334-343, 2019 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-30849653

RESUMO

Ulva ohnoi is a green macroalga with fast growth and high rates of nitrogen and phosphorus absorption. Recently, this species has been recorded in several places with record green tide formation in some of them. Using molecular tools, we herein report the first occurrence of this species in Brazil and demonstrate its potential for phytoremediation in typical environmental concentrations of Cd (0.625-15 µg L-1). Similarly, the effects of physicochemical parameters (salinity and temperature) on the toxicity and uptake efficiency of this species were evaluated. Molecular analysis of two sequences (1141 bp) obtained corroborates another 34 sequences for U. ohnoi obtained from GenBank. The addition of Cd in the medium affected photosynthetic parameters and reduced growth rate. U. ohnoi showed resistance to Cd when cultivated at 18 °C, S15 and 18-25 °C, S35, at concentrations between 0.625 and 2.5 µg. L-1 of Cd; yet, positive growth rate was maintained. Dose-dependent accumulation was observed in all combinations of factors used with a maximum value of 4.20 µg Cd per gram of dry seaweed at 15 µg. L-1 of Cd at 18 °C and S35. Maximum value of the concentration factor was 81.3 ±â€¯1.1% of Cd added at the concentration of 0.625 µg. L-1 to S15 and 18 °C. Our results demonstrate the potential of using U. ohnoi in the phytoremediation of Cd in saltwater or brackish water.


Assuntos
Cádmio/toxicidade , Alga Marinha/efeitos dos fármacos , Ulva/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Biodegradação Ambiental , Brasil , Cádmio/metabolismo , Relação Dose-Resposta a Droga , Nitrogênio/metabolismo , Fósforo/metabolismo , Fotossíntese/efeitos dos fármacos , Salinidade , Alga Marinha/metabolismo , Temperatura , Ulva/metabolismo , Poluentes Químicos da Água/metabolismo
16.
Einstein (Sao Paulo) ; 17(1): eAO4477, 2019 Feb 07.
Artigo em Inglês, Português | MEDLINE | ID: mdl-30758400

RESUMO

OBJECTIVE: To described the allele and haplotype frequencies of human leukocyte antigen genes at the -A, -B loci and human platelet antigen genes for human platelet antigen systems 1 to 9, 11 and 15 in blood. METHODS: We included 867 healthy unrelated volunteer donors who donated platelets between January 2011 and December 2014. Microarray genotyping was performed using a BeadChip microarray. Medium resolution typing of the human leukocyte antigen at loci A and B was carried out using sequence-specific oligonucleotide probe hybridization. We used multivariate analysis and our human leukocyte antigen population was compared to data from the United States national bone marrow donor program. Human platelet antigen results were compared to a literature review and data from around the world. RESULTS: Our human leukocyte antigen haplotype results were more similar to those of hispanics, followed by caucasians. Likewise, our human platelet antigen sample is more similar to those of Argentina, Rio Grande do Sul and Italy. CONCLUSION: This was the first article that discusses human platelet antigen and human leukocyte antigen data together. Rare genotypes or antibody associations can make patient management difficult. A blood bank with genotyped donors allows for optimal transfusion and can contribute to better results. Our information can serve as basis for a database of platelet antigen polymorphisms.


Assuntos
Alelos , Antígenos de Plaquetas Humanas/genética , Técnicas de Genotipagem/métodos , Antígenos HLA/genética , Haplótipos/genética , Polimorfismo Genético/genética , Adolescente , Adulto , Idoso , Feminino , Frequência do Gene/genética , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Transfusão de Plaquetas , Doadores de Tecidos , Adulto Jovem
17.
Obes Surg ; 29(2): 369-375, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30293133

RESUMO

INTRODUCTION: Some traditional bariatric surgery procedures may lead to functional gut shortening, which may unsettle the fine-tuned gastrointestinal physiology and affect gut microbiota balance. PURPOSE: Evaluate the gut microbiota behavior in rat models facing gut shortening due to intestinal bypass. MATERIALS AND METHODS: Wistar rats (n = 17) were randomly distributed in three groups: (1) sham group (n = 5); (2) blind loop group (n = 6); and (3) resection group (n = 6). Intestinal samples and feces were analyzed to measure bacterial concentrations (small intestinal bacterial overgrowth-SIBO) 12 weeks after the experimental procedures. Bacterial translocation (BT) was investigated in the mesenteric lymph node (MLN), liver, spleen, and lung of the animals. In addition, inflammatory aspects were investigated in their liver and small bowel through histological analysis. RESULTS: Regardless of blind loop, gut shortening groups recorded similar high level of bacterial concentrations in intestine compartments, greater than that of the sham group (p ≤ 0.05). BT was only observed in the MLN of gut shortening models, with higher percentage in the blind loop group (p ≤ 0.05). The gut and liver histopathological analysis showed similar low-grade chronic inflammation in both gut shortening groups, likely associated with SIBO/BT events. CONCLUSION: Sustained SIBO/BT was associated with proximal gut shortening in half regardless of blind loop, whereas the GI tract's ability to restore gut microbiota balance after a surgical challenge on the small bowel appears to be linked to the functional remaining gut.


Assuntos
Cirurgia Bariátrica , Disbiose/etiologia , Intestino Delgado/cirurgia , Síndromes de Malabsorção/etiologia , Obesidade Mórbida/cirurgia , Animais , Translocação Bacteriana/fisiologia , Cirurgia Bariátrica/efeitos adversos , Cirurgia Bariátrica/métodos , Disbiose/patologia , Fezes/microbiologia , Microbioma Gastrointestinal/fisiologia , Intestino Delgado/microbiologia , Intestino Delgado/patologia , Síndromes de Malabsorção/patologia , Masculino , Obesidade Mórbida/microbiologia , Obesidade Mórbida/patologia , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/microbiologia , Distribuição Aleatória , Ratos , Ratos Wistar
18.
Einstein (Säo Paulo) ; 17(1): eAO4477, 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-984373

RESUMO

ABSTRACT Objective To described the allele and haplotype frequencies of human leukocyte antigen genes at the -A, -B loci and human platelet antigen genes for human platelet antigen systems 1 to 9, 11 and 15 in blood. Methods We included 867 healthy unrelated volunteer donors who donated platelets between January 2011 and December 2014. Microarray genotyping was performed using a BeadChip microarray. Medium resolution typing of the human leukocyte antigen at loci A and B was carried out using sequence-specific oligonucleotide probe hybridization. We used multivariate analysis and our human leukocyte antigen population was compared to data from the United States national bone marrow donor program. Human platelet antigen results were compared to a literature review and data from around the world. Results Our human leukocyte antigen haplotype results were more similar to those of hispanics, followed by caucasians. Likewise, our human platelet antigen sample is more similar to those of Argentina, Rio Grande do Sul and Italy. Conclusion This was the first article that discusses human platelet antigen and human leukocyte antigen data together. Rare genotypes or antibody associations can make patient management difficult. A blood bank with genotyped donors allows for optimal transfusion and can contribute to better results. Our information can serve as basis for a database of platelet antigen polymorphisms.


RESUMO Objetivo Descrever as frequências alélicas e haplotípicas de genes dos antígenos leucocitários humanos nos loci -A,- B e dos antígenos plaquetários humanos para os sistemas HPA-1 a 9, 11 e 15. Métodos Foram incluídos 867 doadores voluntários, saudáveis, não relacionados, que doaram plaquetas por aférese entre janeiro de 2011 e dezembro de 2014. A genotipagem foi realizada usando microarray BeadChip. A tipificação de resolução intermediária dos antígenos leucocitários humanos loci A e B foi realizada por meio de hibridização com sonda para oligonucleotídeos por sequência específica. Utilizamos análises multivariadas e o antígeno leucocitário humano de nossa população foi comparado com a do programa nacional de doadores de medula óssea norte-americano. Já os resultados dos antígenos plaquetários humanos foram comparados à revisão da literatura e a dados de populações de outros países. Resultados Os resultados do haplótipo de antígenos leucocitários humanos são mais parecidos com os dos hispânicos, seguidos dos caucasianos. Igualmente, a amostra de antígenos plaquetários humanos foi mais semelhante às da Argentina, do Rio Grande do Sul e da Itália. Conclusão Este foi o primeiro artigo a discutir antígenos plaquetários e leucocitários humanos simultaneamente. Genótipos raros ou associações de anticorpos podem dificultar o manejo clínico do paciente. Um banco de sangue com doadores genotipados permite um melhor resultado e transfusão possíveis. Estas informações podem servir de base para um banco de dados sobre polimorfismos de antígenos plaquetários.


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Polimorfismo Genético/genética , Haplótipos/genética , Antígenos de Plaquetas Humanas/genética , Alelos , Técnicas de Genotipagem/métodos , Antígenos HLA/genética , Doadores de Tecidos , Transfusão de Plaquetas , Frequência do Gene/genética , Genótipo
19.
J Pharm Pharm Sci ; 21(1): 376-385, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30342562

RESUMO

PURPOSE: Flavonoids are a group of secondary metabolites of the polyphenols class present in several plant species. Among them, the biflavonoid agathisflavone is of interest since it bears several biological effects that include: antiviral, antitumoral, antiprotozoal and neurogenic actions. In this sense, this study aims to use the important tool of scientific prospecting to assess the level of research development concerning the flavonoid agathisflavone. METHODS: The experimental design was carried out through strategic reach with keywords on the PubMed (National Center for Biotechnology Information - NCBI) and Science Direct platforms. The articles were compiled and exported to Microsoft Office Excel 2007, where they were analyzed, stored and distributed in charts organized as to different countries, year of publication of scientific articles and journals RESULTS: The prospective research resulted in the identification of 81 scientific productions, published in several journals, submitted by different countries, in several areas of medical domain and in different years of publication over the last 50 years (1965 - 2018). It was also possible to investigate the advances in the study of agathisflavone for the development of new therapeutics. CONCLUSION: Although agathisflavone has been known in the literature since at least 1969, only 23 of the eligible articles found evaluated its possible therapeutic effects. The demonstrated biological activities of agathisflavone range from antiprotozoal to neurogenesis and neuroprotection, however, the molecule needs to be better studied at the in vivo and human level.


Assuntos
Anti-Infecciosos/farmacologia , Antineoplásicos/farmacologia , Antiprotozoários/farmacologia , Biflavonoides/farmacologia , Fármacos Neuroprotetores/farmacologia , Anti-Infecciosos/síntese química , Anti-Infecciosos/química , Antineoplásicos/síntese química , Antineoplásicos/química , Antiprotozoários/síntese química , Antiprotozoários/química , Biflavonoides/síntese química , Biflavonoides/química , Desenho de Fármacos , Humanos , Fármacos Neuroprotetores/síntese química , Fármacos Neuroprotetores/química , Estudos Prospectivos
20.
Rev Bras Hematol Hemoter ; 40(1): 25-29, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29519368

RESUMO

INTRODUCTION: Pre-transfusion tests, essential for the release of blood components, may be affected by drugs. Monoclonal antibodies represent a class of medications increasingly used in the clinical practice, with anti-CD38 monoclonal antibodies (daratumumab) being a promising resource in the treatment of refractory myeloma. This monoclonal antibody recognizes CD38 in myeloma cells and interferes with pre-transfusion tests by causing panreactivity in indirect antiglobulin tests thereby clinically masking alloantibodies. Dithiothreitol is a reagent that breaks disulfide bonds and effectively destroys antigenic sites for CD38 on red blood cells. This study reports the immunohematological findings of pre-transfusion tests of patients with multiple myeloma receiving daratumumab and on solutions to prevent the interference of this monoclonal antibody. METHODS: Serum samples from five patients on anti-CD38 monoclonal antibody treatment were evaluated. Tests performed included ABO/RhD typing, indirect antiglobulin test, direct antiglobulin test and eluate test. A daily evaluation was performed to determine the shelf life of dithiothreitol-treated red blood cells when stored in Alsever's solution. RESULTS: No interference in the ABO/RhD typing results was noted but in all samples, a panreactivity was observed in indirect antiglobulin tests. Regarding the direct antiglobulin test, two samples presented positive results but negative eluates. In all samples, treatment of reagent red blood cells with 0.2M dithiothreitol offset interference by anti-CD38 monoclonal antibodies. Dithiothreitol-treated red blood cells stored in Alsever's solution were stable for up to 15 days. CONCLUSION: Treatment of reagent red blood cells with dithiothreitol can be efficient and accessible to offset the interference of the anti-CD38 drug in pre-transfusion tests. The number of costly serological workups can be reduced by having stored dithiothreitol red blood cells with this proving to be a useful reagent for investigating anti-CD38.

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