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Int J Pharm ; 597: 120296, 2021 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-33524524


The neonatal and pediatric populations have long been neglected concerning the development of oral dosage forms. For close to two decades, caregivers have had to adjust the doses of the off-label medicines and drugs for adults to suit the neonatal and pediatric needs. This is due to the lack of rules and regulations regarding neonates and pediatrics clinical trials while pharmaceutical industries see this as a non-lucrative approach. Despite such limitations, the administration of solid and liquid dosage forms to neonates and pediatrics necessitates the development of new technologies and even new strategies to meet the needs. Current approaches have not only focused on the development of suitable dosage forms but also the advancement of devices to enhance drug administration to pediatrics and neonates. Though current approaches have significantly added to the number of pediatric and neonatal oral dosage formulations on the market, there is still more room for improvement(s). While novel dosage forms including multiparticulates, orodispersible tablets/films, and chewable tablets have extensively been researched, some administration devices (e.g., nipple shield, pill swallowing cup, and solid dosage pen) have also been explored. Although a few of these products are in the market, the concerted efforts of regulation administrative bodies, pharmaceutical industry settings, and scientists in academia have been oriented to address all issues and advance the neonatal and pediatric-centric pharmaceutical products.

Environ Toxicol Pharmacol ; 80: 103473, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32860936


Residual contamination of water with MPH represents a severe environmental issue because it can affect non-target animals. Here we describe the behavioral effects in zebrafish after chronic contamination of water containing residues of MPH (0.1875, 1.875 and 3 ug/L). These doses are environmentally relevant since they reflect those found in wastewaters. We evaluated the behavioral effect through the novel tank test (NTT) and social preference test (SPT), and after euthanasia we analyzed oxidative stress parameters. Zebrafish exposed to MPH presented a social impairment, avoiding the conspecifics segment in the social preference test. In addition, MPH in the lowest concentration provoked an anxiolytic effect in the novel tank test. Oxidative stress is not related to these changes. Since the maintenance of an intact behavioral repertoire is crucial for species survival and fitness, our results demonstrate that residual contamination of water by MPH can be a threat to zebrafish, impacting directly to its well-being and survival in the aquatic environment.

Med Gas Res ; 10(1): 54-59, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32189671


Acute or chronic inflammatory reactions aim to control lesions, resist to pathogens attack and repair damaged tissue. The therapeutic administration of ozone known as ozone therapy appears as a possible treatment for tissue repair, as it promotes the healing of wounds. It has bactericidal, antiviral and antifungal properties and has been used as a therapeutic resource to treat inflammation. The objective was to carry out an integrative review regarding the use of ozonated oil in acute and chronic inflammations. The keywords "ozone therapy," "inflammation" and "ozone" were used in the Portuguese, Spanish and English languages. The paper selection was based on inclusion and exclusion criteria. In total, 28 articles were selected. It has been seen that ozonated oil is effective in healing cutaneous wounds. The beneficial effects are due to the healing of wounds, due to the reduction of microbial infection, debridement effect, modulation of the inflammatory phase, stimulation to angiogenesis as well as biological and enzymatic reactions that favor the oxygen metabolism, improving the wound cicatrization. In addition to promoting healing, ozonated oil reduces symptoms related to skin burns, prevents post-lesion hyperpigmentation, and reduces the pain of aphthous ulcers. Therefore, ozonated oil represents an effective and inexpensive therapeutic alternative that must be implanted in the public health system.

Ciênc. rural (Online) ; 50(4): e20190689, 2020. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1089578


ABSTRACT: Pomegranate (Punica granatum L.) contains a high concentration of antioxidant and phenolic compounds. Pomegranate peel extracts at different concentrations were used as natural antioxidant to increased the lipid stability of fresh Tuscan sausages, comparing with sodium erythorbate (SE). Peels were subjected to ultrasound-assisted aqueous extraction and lyophilization. The extract was previously characterized throught phenol and flavonoids determination. The Tuscan Sausages were prepared, and color, pH, peroxides, and thiobarbituric acid reactive substances were assessed at 1, 15, and 30 days of storage, at a mean temperature of 5 ± 1 ºC and under a 12-hour light cycle/day. Sausages containing 0.05 and 0.1% of peel extract showed results similar to sodium erythorbate in lipid peroxidation. Sausages treated with pomegranate peel extracts showed an adequate global acceptability level in the sensory analysis. Pomegranate peel extracts are; therefore, a promising natural alternative for maintaining the lipid stability of Tuscan sausages, promoting the protection of the meat and in addition, being able to bring beneficial of the pomegranate to the human health.

RESUMO: A romã (Punica granatum L.) contém uma alta concentração de compostos antioxidantes e fenólicos. Extratos de casca de romã em diferentes concentrações foram utilizados como antioxidante natural para aumentar a estabilidade lipídica de linguiça tipo Toscana, comparados ao eritorbato de sódio. As cascas foram submetidas à extração e liofilização aquosas por ultrassom. O extrato foi caracterizado por determinação de fenol e flavonóides. Os parâmetros cor, pH, peróxidos e ácido tiobarbitúrico foram avaliadas nos dias 1, 15 e 30 pós armazenamento, a temperatura de 5 ± 1 ºC e ciclo de luz de 12 horas/dia. As linguiças contendo 0,05 e 0,1% de extrato de casca apresentaram resultados semelhantes ao eritorbato de sódio quanto a peroxidação lipídica. Também apresentaram aceitabilidade global adequada na análise sensorial. Os extratos de casca de romã são, portanto, uma alternativa natural promissora para manter a estabilidade lipídica das linguiças toscanas, promovendo a proteção e podendo trazer benefícios da romã à saúde humana.

Adv Pharm Bull ; 9(2): 241-248, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31380249


Purpose: We aimed to evaluate the effect of blueberry extract and microparticles (MP) on sunscreen performance of multifunctional cosmetics. Octocrylene (OCT), benzophenone-3 (BENZ-3) and Tinosorb® M (MBBT) were employed as UV filters. Methods: An in-silico modeling was used to determine the UV filters concentrations to obtain high values of sunscreen protection factor (SPF) and UVA protection factor (UVA-PF). MBBT and blueberry-loaded microparticles (MPMB+B) and MBBT-loaded microparticles (MPMBBT) were prepared by spray-drying. OCT and BENZ-3 were added in the oil phase of cosmetics. Cosmetics A and B contained MPMB+B and MPMBBT, respectively, and cosmetic C was prepared without MP. Characterization, physicochemical stability and in vitro SPF was performed. UV filters distribution in human stratum corneum (SC) for each cosmetic was performed. Anti-oxidant activity of blueberry extract was evaluated. Results: Sunscreen combination with the highest SPF was selected for formulations. Formulations A and B maintained their rheological behavior over time, unlike formulation C. In-vitro SPFs for formulations A, B and C were 51.0, 33.7 and 49.6, respectively. We also developed and validated a method for analysis of the UV filters by HPLC/ PDA suitable for the in-vivo assay. In Tape stripping test, MBBT showed SC distribution similar for all cosmetic formulations. OCT and BENZ-3 distribution to formulation A and C was also similar. Blueberry extract showed antioxidant capacity of 16.71 µg/mL equivalent to vitamin C. Conclusion: Cosmetics containing MPs presented better physical stability. Blueberry increased the photoprotective capacity of the formulations and added extra benefits due to its anti-oxidant and anti-aging properties.

Biomed Chromatogr ; 33(9): e4564, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31041812


Midazolam (MDZ) is the first choice in palliative sedation, and commonly used in sleep induction in anesthesia, with rapid onset of action. However, monitoring of the level of sedation in patients is not accurate. We developed and validated a bioanalytical method to detect MDZ in plasma using high-performance liquid chromatography (HPLC) coupled to a photodiode array detector (PDA) for future monitoring of sedation. MDZ was extracted by solid-phase extraction (SPE). Analyses were performed on a C18 column, using 0.05% triethylamine and acetonitrile as mobile phase, analyzing at 220 nm. Recovery was evaluated by comparing extracted and nonextracted solutions. Precision, accuracy, linearity, limits of detection (LD) and quantification (LQ), specificity and selectivity were determined. The mean recovery obtained by SPE was 101.03%. The method was linear in the range 1.0-50.0 µg/mL. The LD and LQ were, respectively, 0.43 and 1.43 µg/mL. The specificity of the MDZ peak was adequate. The method was able to detect MDZ among other drugs. Plasma anticoagulants showed no interference with the drug detection. The bioanalytical method using HPLC-PDA and SPE was successfully validated and showed linearity, precision, accuracy, specificity and high sensitivity for detection of MDZ in human plasma.

Cromatografia Líquida de Alta Pressão/métodos , Midazolam/sangue , Extração em Fase Sólida/métodos , Humanos , Limite de Detecção , Modelos Lineares , Reprodutibilidade dos Testes
Aust Endod J ; 45(2): 209-215, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30230647


The aim of this study was to evaluate the influence of ultrasonic activation (US) over final irrigants on antimicrobial activity, smear layer removal and bond strength. The root canals of 180 extracted human teeth were distributed into three experimental tests (n = 60). In each test, the samples were subdivided into six groups (n = 10) according to final irrigation protocols: G1:distilled water (DW); G2:DW + US; G3:17% EDTA; G4:QMix; G5:17% EDTA + US; and G6:QMix + US. The antimicrobial activity was evaluated by counting of colony-forming units (CFUs), the smear layer removal by scanning electron microscopy and the bond strength by push-out test. Statistical analysis was performed for each evaluation (α = 5%). Groups 4 and 6 provided higher bacterial reduction, groups 5 and 6 provided higher smear layer removal, groups 3, 4, 5 and 6 provided the higher bond strength (P < 0.05). US activation did not improve the antimicrobial activity and the bond strength, whereas it improved the smear layer removal.

Anti-Infecciosos , Camada de Esfregaço , Cavidade Pulpar , Ácido Edético , Humanos , Microscopia Eletrônica de Varredura , Irrigantes do Canal Radicular , Ultrassom
Photodiagnosis Photodyn Ther ; 23: 347-352, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30053480


INTRODUCTION: The aim of this study is to evaluate the antimicrobial activity of hypochlorite solutions and reciprocating instrumentation associated with photodynamic therapy (PDT). METHODS: One hundred and thirty two root canals were enlarged up to #35 K-file and inoculated with E.faecalis for 14 days. The 132 samples were randomly divided into eleven groups (n = 12) and subjected to the following protocols: G1-distilled water + Reciproc R40 (control), G2-1% sodium hypochlorite (NaOCl) + Reciproc R40, G3-2.5% NaOCl + Reciproc R40; G4-1% calcium hypochlorite (Ca[OCl]2) + Reciproc R40, G5-2.5% Ca(OCl)2 + Reciproc R40; G6-PDT; G7-distilled water + Reciproc R40 + PDT, G8-1% NaOCl + Reciproc R40 + PDT, G9-2.5% NaOCl + Reciproc R40 + PDT; G10-1% Ca(OCl)2 + Reciproc R40 + PDT, G11-2.5% Ca(OCl)2 + Reciproc R40 + PDT. The percentage bacterial reduction was checked by counting the colony-forming units (CFUs) in 10 samples of each group. The remaining 2 samples of each group were submitted to scanning electronic microscopy (SEM) to illustrate the effectiveness of the proposed treatments. Data were subjected to one-way ANOVA followed by Tukey's post hoc test (α = 0.05). RESULTS: The greatest ability to promote bacterial reduction was observed in groups 8 (1% NaOCl + R40 + PDT), 9 (2.5% NaOCl + R40 + PDT), 10 (1% Ca[OCl]2 + R40 + PDT), and 11 (2.5% Ca[OCl]2 + R40 + PDT), with no significant difference between them (p < 0.05). CONCLUSIONS: The association of PDT with hypochlorite solutions and reciprocating instrumentation provides effective elimination of E.faecalis.

Enterococcus faecalis/efeitos dos fármacos , Ácido Hipocloroso/uso terapêutico , Azul de Metileno/uso terapêutico , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/uso terapêutico , Preparo de Canal Radicular/métodos , Carga Bacteriana , Compostos de Cálcio/uso terapêutico , Terapia Combinada , Cavidade Pulpar/microbiologia , Relação Dose-Resposta a Droga , Humanos , Hipoclorito de Sódio/uso terapêutico
BrJP ; 1(2): 171-175, Apr.-June 2018. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1038921


ABSTRACT BACKGROUND AND OBJECTIVES: Osteoarthrosis affects 85% of the population over 75 years of age. It is divided into primary and secondary, however despite the knowledge at the molecular level the treatments are not yet fully effective. However, ozone therapy emerges as an alternative therapy, which is low cost and seems effective in the treatment of chronic pain. The objective of this study was to evaluate the current evidence to support or to refute the use of ozone therapy in the treatment of patients with osteoarthritis. CONTENTS: Systematic review using the keywords "ozone therapy", "ozone", "osteoarthritis", "arthritis", "randomized", "controlled" and "meta-analysis". The selection of publications was based on inclusion and exclusion criteria. In total, 9 articles were used. Among the 9 articles found regarding ozone therapy in osteoarthritis, 7 of them clearly show the benefits of ozone. The concentrations of ozone used in the studies ranged from 20µg/mL to 15g/mL. The route of administration was intra-articular and rectal insufflation. The frequency of use was, on average, 1 to 3 times a week and the treatment time was between 3 to 4 months in most of the studies. CONCLUSION: The use of ozone produces clinically relevant benefits in patients with osteoarthrosis. Therefore, ozone therapy in osteoarthrosis represents a low-cost, efficient therapeutic alternative that should be implemented in the country's Public Health, considering the prevalence of the disease.

RESUMO JUSTIFICATIVA E OBJETIVOS: A osteoartrose atinge 85% da população com mais de 75 anos. A mesma é dividida em primária e secundária, porém apesar do conhecimento a nível molecular, os tratamentos ainda não são totalmente eficazes. Entretanto, a ozonioterapia, é de baixo custo e parece efetiva no tratamento da dor crônica. O objetivo deste trabalho foi avaliar as evidências atuais que apoiem ou refutem o uso da ozonioterapia no tratamento de pacientes com osteoartrose. CONTEÚDO: Revisão sistemática, utilizando as palavras-chave: "ozone therapy", "ozone", "osteoarthritis", "arthritis", "randomised", "controlled" e "meta-analysis". A seleção das publicações foi feita a partir de critérios de inclusão e exclusão. Ao total foram utilizados 9 artigos. Dentre os 9 artigos encontrados a respeito de ozonioterapia na osteoartrose, 7 deles mostraram claramente os benefícios do ozônio. As concentrações de ozônio utilizadas nos estudos variaram de 20µg/mL a 15g/mL. A via de administração utilizada foi a intra-articular e a insuflação retal. A frequência do uso foi, em média, de 1 a 3 vezes por semana e o tempo de tratamento foi entre 3 a 4 meses na maior parte dos estudos. CONCLUSÃO: O uso do ozônio produz benefícios clinicamente relevantes em pacientes com osteoartrose, portanto a ozonioterapia na osteoartrose representa uma alternativa terapêutica de baixo custo, e eficiente, que deve ser implantada na Saúde Pública do país, tendo em vista a prevalência da doença.

Clin Oral Investig ; 22(2): 859-865, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28669105


OBJECTIVES: Chlorhexidine binds to dentine to provide sustained action. The aim of the present study was to evaluate and compare the substantivity of chlorhexidine using manual, rotary, and reciprocating systems for root canal preparation. METHODS: Forty-five extracted human single-rooted teeth were used for this study. The samples were divided into three groups (n = 15) according to the instrumentation technique used: manual instrumentation (K-File), rotary instrumentation (ProTaper), and reciprocating instrumentation (Reciproc R25). Chlorhexidine gel (2%) was used as auxiliary chemical substance during root canal preparation. Longitudinal grooves were carved on the free surfaces of the roots, providing two halves of each root and resulting in 30 samples per group. Each group was randomly divided into three subgroups (n = 10), and substantivity was evaluated after 48 h, 7 days, and 30 days of incubation. The amount of CHX (in mg/mL) was measured through reverse-phase high-performance liquid chromatography. Statistical analysis was performed by analysis of variance and the Tukey test for post hoc comparisons (α = 0.05). RESULTS: The manual did not show a statistical significant difference with rotary instrumentation (P > 0.05), but higher CHX substantivity was recorded in all periods of observation when compared to reciprocating instrumentation (P < 0.05). CONCLUSIONS: The CHX substantivity on human dentine is lowest when using reciprocating compared to manual and rotary instrumentation. CLINICAL SIGNIFICANCE: Using chemical analysis, this study showed that a manual and rotary multi-instrument system results in greater chlorhexidine substantivity on human dentin than a reciprocating single-instrument system.

Anti-Infecciosos Locais/química , Clorexidina/química , Dentina/efeitos dos fármacos , Preparo de Canal Radicular/métodos , Adulto , Cromatografia Líquida de Alta Pressão , Humanos , Técnicas In Vitro , Distribuição Aleatória , Irrigantes do Canal Radicular/química , Preparo de Canal Radicular/instrumentação
Pharmacogn Mag ; 13(Suppl 2): S370-S374, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28808408


BACKGROUND: The traditional use of Drimys brasiliensis Miers (Winteraceae) in the south of Brazil to reduce cholesterol has not been described in scientific literature. OBJECTIVE: To verify the hypocholesterolemic effects of D. brasiliensis using rats as animal model. MATERIALS AND METHODS: The bark of D. brasiliensis was extracted with water with further lyophilization and was subjected to phytochemical analysis by high-performance liquid chromatography (HPLC), and free radical scavenging activities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay to determine antioxidant potential. The hypocholesterolemic activity was determined in male Wistar rats treated with 100 and 250 mg/kg/day extract concomitantly fed a hypercaloric diet, over 20 days (prevention assay). In the treatment assay, rats were fed a hypercaloric diet for 40 days and received the extract (100 mg/kg/day) from day 20. RESULTS: In this research, we found that the extract of the bark of D. brasiliensis was able to reduce the triglycerides significantly and reduce total cholesterol at doses 100 and 250 mg/kg/day and both administration regimens (prevention and treatment) in rats treated with the extract and hypercaloric diet. The extract showed strong antioxidant properties (DPPH assay), probably responsible by hypocholesterolemic activity of the plant. By HPLC, we detected catechin (1.34%), epicatechin (3.48%), rutin (0.86%), caffeic acid (0.45%), and ferulic acid (0.84%) in D. brasiliensis extract. CONCLUSIONS: We confirm the popular use of the plant to reduce of cholesterol. SUMMARY: The extract of the bark of Drimys brasiliensis was able to reduce the triglycerides significantly and reduced total cholesterol at doses 100 and 250 mg/kg/day and both administration regimens (prevention and treatment) in rats treated with the extract and hypercaloric dietThe extract showed strong antioxidant properties (1,1-diphenyl-2-picrylhydrazyl assay), probably responsible by hypocholesterolemic activity of the plantThe extracts present catechin (1.34%), epicatechin (3.48%), rutin (0.86%), caffeic acid (0.45%), and ferulic acid (0.84%)The plant can be used to cholesterol reduction. Abbreviations used: HPLC: High-performance liquid chromatography; PDA: Photodiode array detector; RS: Reference substances; DPPH: 1,1-diphenyl-2-picrylhydrazyl; VCEAC: Vitamin C equivalent antioxidant capacity.

Environ Sci Pollut Res Int ; 24(28): 22673-22678, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28812184


Ozone helps decontamination environments due to its oxidative power, however present toxicity when it is in high concentrations, by long periods of exposition. This study aimed to assess the safety of ozone generator air purifier at concentrations of 0.05 ppm in rats exposed to 3 and 24 h/day for 14 and 28 days. No significant differences are observed between groups in clinical signs, feed and water intake, relative body weight gain and relative weight of organs, macroscopy and microscopy of lungs, and oxidative plasma assay. In this exposure regime, ozone does not cause genotoxicity and no significant changes in pulmonary histology indicative of toxicity. Ozone generated in low concentrations, even in exposure regimes above the recommended is safe, both acute and sub-acute exposition.

Ar Condicionado/normas , Ozônio/análise , Ozônio/toxicidade , Ar Condicionado/instrumentação , Animais , Peso Corporal/efeitos dos fármacos , Células da Medula Óssea/efeitos dos fármacos , Células da Medula Óssea/patologia , Ensaio Cometa , Relação Dose-Resposta a Droga , Exposição por Inalação , Pulmão/efeitos dos fármacos , Pulmão/patologia , Masculino , Testes para Micronúcleos , Estresse Oxidativo/efeitos dos fármacos , Ratos Wistar , Testes de Toxicidade Aguda , Testes de Toxicidade Subaguda
Neurosci Lett ; 650: 114-117, 2017 05 22.
Artigo em Inglês | MEDLINE | ID: mdl-28445770


Several studies have reported the presence of methylphenidate (MPH) in effluents; however, its impacts on aquatic life are not yet well understood. Here, we investigated the effects of acute exposure on endocrine and behavioral stress responses to environmentally relevant concentrations of MPH in zebrafish. We show that MPH blunts the response of cortisol to stress in zebrafish. On the other hand, MPH stresses fish per se. Additionally, MPH seems to modulate anxiety-like behaviors. We conclude that the presence of MPH in aquatic environments can alter neuroendocrine and behavior responses, which might considerably impact fish survival and welfare.

Comportamento Animal/efeitos dos fármacos , Comportamento Animal/fisiologia , Hidrocortisona/metabolismo , Metilfenidato/administração & dosagem , Estresse Fisiológico/efeitos dos fármacos , Poluentes Químicos da Água/administração & dosagem , Peixe-Zebra/fisiologia , Animais , Estimulantes do Sistema Nervoso Central/administração & dosagem , Relação Dose-Resposta a Droga , Sistema Endócrino/efeitos dos fármacos , Sistema Endócrino/fisiologia , Feminino , Humanos , Estresse Fisiológico/fisiologia
J AOAC Int ; 100(2): 400-405, 2017 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-28118563


Stability-indicating LC methods using a UV detector and a charged aerosol detector (CAD) simultaneously were validated for the assessment of alogliptin (ALG) in tablets. The analysis was performed on a C8 column (250 × 4.6 mm, 5 µm) at a flow of 0.8 mL/min, using acetonitrile-10 mM ammonium acetate buffer (pH 3.5; 90 + 10, v/v) as mobile phase and UV detection at 275 nm. Validation followed the International Conference on Harmonization guidelines. The method was linear over the range of 25-200 µg/mL. Normality of the residuals showed a normal distribution, no autocorrelation, and homoscedasticity. LODs were 6.25 and 2.65 µg/mL and LOQs were 20.85 and 8.84 µg/mL for the CAD and the UV detector, respectively. The methods were precise and accurate. Excipients and degradation products did not interfere in the methods in studies of specificity. None of the factors studied in the analysis of robustness had a significant effect on the quantification of the ALG by the Pareto chart. The results of the assay obtained with LC-CAD and LC-UV were similar. The methods could be considered interchangeable and stability-indicating, and can be applied as an appropriate QC tool for analysis of ALG in tablets.

Piperidinas/análise , Uracila/análogos & derivados , Ácido Benzoico/análise , Cromatografia Líquida , Estabilidade de Medicamentos , Excipientes/análise , Limite de Detecção , Manitol/análise , Piperidinas/administração & dosagem , Comprimidos , Uracila/administração & dosagem , Uracila/análise
Rev. odontol. UNESP (Online) ; 45(2): 85-89, mar.-abr. 2016. graf
Artigo em Português | LILACS-Express | LILACS | ID: lil-780067


Resumo Introdução A avaliação microbiológica em produtos de higiene pessoal constitui uma etapa importante no que se refere à segurança do usuário e à qualidade do produto, visto que a carga microbiana elevada pode acarretar problemas de saúde, especialmente em pessoas imunocomprometidas. Objetivo Verificar o cumprimento das exigências acerca da qualidade microbiológica de cremes e géis dentais adquiridos comercialmente. Material e método Realizou-se a contagem de bactérias e fungos viáveis totais e pesquisa dos patógenos E. coli, Salmonella sp., S. aureus e P. aeruginosa em 21 amostras. Resultado Das amostras analisadas, 52,0% apresentaram crescimento microbiano e 28,6% e 0,21% apresentaram contaminação fúngica e bacteriana, respectivamente, acima dos limites descritos na Farmacopeia Brasileira para preparações de uso tópico (máximo permitido 2 × 102 UFC/g de bactérias e 2 × 101 UFC/g de fungos). Nenhuma amostra apresentou os patógenos pesquisados E. coli, Salmonella sp., S. aureus e P. aeruginosa. Conclusão Estes resultados indicam que muitos produtos disponíveis no mercado apresentam qualidade inadequada, demonstrando falhas no controle de qualidade. Para prevenir esta situação, faz-se necessária fiscalização rigorosa e adoção de medidas regulamentadoras e educacionais aliadas ao seguimento das Boas Práticas de Fabricação pelas indústrias fabricantes.

Abstract Introduction Microbiological evaluation in personal care products is important to guarantee user safety and product quality since microbial contamination elicits health problems especially in immunocompromised patients. Objective To verify the compliance with the requirements regarding the microbiological quality of creams and dental gels acquired commercially. Material and method Microbiological contamination was performed through total bacterial and fungal viable count and research of E. coli, Salmonella sp., S. aureus and P. aeruginosa in twenty-one samples. Result 52.0% of sample presented microbial growth, and 28.6% and 0.21% presented fungal and bacterial contamination, respectively, exceeding the limits described in Brazilian Pharmacopoeia for topical preparations (maximum allowable 2 × 102 CFU / g of bacteria and 2 × 101 CFU / g yeast). None of the researched pathogens E. coli, Salmonella sp., S. aureus and P. aeruginosa were found. Conclusion These results indicate that many products available in the market present inadequate quality, demonstrating quality control failures. Rigorous inspection and adoption of regulatory and educational measures aligned with the compliance of Good Manufacturing Practices by manufactures are needed to prevent this situation.