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1.
Vet Res Commun ; 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-35059959

RESUMO

Reduced welfare and productivity of dairy goats have often been associated with poor claw health, especially conditions such as claw overgrowth and deformations. It is known that periodic claw trimmings have prophylactic and therapeutic effects on these problems, and this study aimed to evaluate if the additional use of an angle grinder to finish trimming overgrown and deformed goat claws, after the usual trimming using hoof shears, could provide further changes in these claws. For this, twelve Saanen goats (57.29 ± 11.15 kg of body weight, 3.08 ± 1.78 years old) were selected by presence of severe claw overgrowth, and absence of claw alterations of other nature. Their claws were trimmed in two steps, first using hoof shears and then using an angle grinder. Morphometric, baropodometric, and conformational aspects of all claws were assessed before claw trimming and after each trimming step. To analyse the effects of the trimming steps in each claw, the Tukey's test was used on parametric data, with 5% probability, and descriptive statistics were used on non-parametric data. Although this is a small pilot study, results suggest that using an angle grinder after the use of hoof shears, could further reduce heel length and sole width of claws, as well as reduce the number of deformed claws. The incorporation of the second trimming tool, could also further increase the frequency with which the point of maximum pressure was found in the toes, rather then in the heels of the claws as seen in deformed claws.

2.
Res Vet Sci ; 135: 167-174, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33524827

RESUMO

Metritis in dairy cows is classified as a puerperal disease that affects all layers of the uterine epithelium and occurs during the first 21 days after parturition. The objectives of this study were to identify potential predictive biomarkers of metritis, to monitor the biochemical profile of pre-metritic dairy cows, and to evaluate the reproductive performance of dairy cows with metritis. Fifty-five cows without metritis and fifteen cows that showed clinical signs of metritis were enrolled in this study. Blood samples were collected at -21, -14 and - 7 days before calving and at parturition and assayed for urea, creatinine, aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), total cholesterol, fibrinogen, total calcium, ß-hydroxybutyrate (BHB) and non-esterified fatty acids (NEFA) concentrations. Serum fibrinogen on 21, 14 and 7 days prepartum and at parturition was the strongest discriminator between metritic and control cows. Moreover, metritic cows had lower (P < 0.05) concentrations of urea, creatinine, cholesterol and calcium and higher (P < 0.05) serum AST, GGT activities, and serum fibrinogen, BHB and NEFA concentrations when compared to control cows. The number of days to first ovulation, insemination, services per pregnancy and days to pregnancy were greater (P < 0.05) for metritic cows than the control group, and the follicle size was smaller (P < 0.05) for metritic cows than the control group. Our results indicate alterations in the biochemical profile of dairy cows with metritis and the negative impact on the reproductive performance of metritic cows raised in tropical conditions. In addition, this study showed that the serum fibrinogen could be used as screening biomarkers to indicate cows that might have metritis during the postpartum period.


Assuntos
Doenças dos Bovinos/sangue , Endometrite/veterinária , Parto , Animais , Biomarcadores/sangue , Estudos de Casos e Controles , Bovinos , Doenças dos Bovinos/diagnóstico , Endometrite/sangue , Feminino , Lactação , Período Pós-Parto/sangue , Valor Preditivo dos Testes , Gravidez , Reprodução , Clima Tropical
3.
Braz. J. Vet. Res. Anim. Sci. (Online) ; 58: e183731, 2021. ilus, tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1344763

RESUMO

This study aimed to verify if the process of artificial insemination (AI) characterized here as animal immobilization, the passage of the semen applicator through the cervix, and deposition of the semen in the uterus, affected cows' welfare. For this, 18 beef calved cows were selected and divided into two groups: inseminated cows (AIG, n = 9), and not inseminated cows, the control group (CG, n = 9). Body condition score, uterus, and ovary evaluation were performed. Later, both groups were submitted into an estrus synchronization protocol and only the AIG group was inseminated. Blood components of urea, creatinine, AST, GGT, CK, glucose, triglycerides, cholesterol, HDL, LDL, VLDL, NEFA, BHB, cortisol, estradiol, progesterone, albumin, and total protein were measured 30 h before AI, and 4, 24, 48 and 168 h after AI. Statistical differences were considered when P <0.05. No differences between AIG and CG were observed. On the other hand, when the moment of insemination was evaluated, differences were observed for urea, creatinine, AST, GGT, CK, glucose, triglycerides, NEFA, BHB, albumin, and total protein. There was an oscillation of metabolic profiles depending on the time and procedures to which animals were exposed, even though it could be inferred that the AI process was incapable of altering those metabolic components on animals that were inseminated. Still, we can affirm that artificial insemination cannot be categorized as a negative reproduction tool on animal welfare. However, the containment and management procedures for AI may alter the metabolic profile of cows, especially the increase of CK.(AU)


O objetivo deste estudo foi verificar se o processo de inseminação artificial (IA) caracterizado como imobilização do animal, passagem do aplicador de sêmen pelo colo do útero e deposição do sêmen no útero, afetou o bem-estar de bovinos. Para tanto, foram selecionadas 18 vacas de corte paridas, divididas em dois grupos: grupo de animais inseminados (AIG, n = 9) e grupo de animais não inseminados, grupo controle (GC, n = 9). Foram avaliados o escore de condição corporal, útero e ovário. Posteriormente, ambos os grupos foram submetidos a um protocolo de sincronização de cio e apenas o grupo AIG foi inseminado. Componentes metabólicos como ureia, creatinina, AST, GGT, CK, glicose, triglicerídeos, colesterol, HDL, LDL, VLDL, NEFA, BHB, cortisol, estradiol, progesterona, albumina e proteína total foram mensurados 30 horas antes da IA e 4, 24, 48 e 168 horas após a IA. Diferenças estatísticas foram consideradas quando P <0,05. Não foram observadas diferenças entre os dois grupos, por outro lado, quando o momento da inseminação foi avaliado, diferenças foram observadas para ureia, creatinina, AST, GGT, CK, glicose, triglicerídeos, NEFA, BHB, albumina e proteína total. Houve uma variação dos perfis metabólicos em função do tempo e dos procedimentos que os animais foram submetidos, embora pode-se inferir que o processo de IA não foi capaz de alterar esses componentes metabólicos os animais inseminados. Ainda assim, observou-se que o processo de IA não foi categorizado como uma ferramenta negativa de reprodução com relação ao bem-estar animal. Porém, ainda assim, os procedimentos de contenção e manejo da IA podem alterar o perfil metabólico das vacas, principalmente o aumento da CK.(AU)


Assuntos
Animais , Feminino , Bovinos , Bem-Estar do Animal , Bovinos/embriologia , Inseminação Artificial/veterinária , Interação Humano-Animal , Metabolismo
4.
Ciênc. rural (Online) ; 51(7): e20200627, 2021. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1249540

RESUMO

ABSTRACT: This study evaluated the viability of Nellore cloned calves derived from somatic cell nuclear transfer (SCNT) and compare their viability with animals of the same breed derived from in vitro fertilization (IVF). Thus, two groups were formed. Group I (GI) consisted of 10 calves derived from SCNT and group II (GII) consisted of 10 calves derived from IVF. The differences detected between the groups were in the physical examination of the respiratory tract in GI, which represented the most common clinical-pathological disturbances. The Apgar index score indicated that 80% of GI animals were depressed and all had pale mucous membranes. Thus, anemia was reported in GI. In GII, this started at 12 h of life and was probably caused by an iron deficiency. Moreover, total calcium and ionized calcium levels were higher in GI immediately after birth. These alterations probably resulted in a high incidence of mortality in GI, reaching 90% of the calves, whereas mortality was only 20% for the calves in GII. In conclusion, cloned calves, which were derived from SCNT, had physiological and metabolic alterations after delivery, leading to a higher mortality rate during the perinatal period.


RESUMO: O objetivo deste trabalho foi avaliar a viabilidade de bezerros da raça Nelore oriundos da técnica da transferência nuclear de células somáticas (TNCS), no período pós natal imediato, comparando-a com animais desta mesma raça, oriundos de fertilização in vitro (FIV). Para tanto, os animais foram alocados em dois grupos, a saber: Grupo I (GI) - 10 animais frutos de TNCS; e, Grupo II (GII) - 10 animais oriundos de FIV. Nos respectivos bezerros, todos obtidos por cesariana, foram realizadas as avaliações físicas, escore de APGAR, bem como coleta de amostras de sangue nos momentos 0 (ao nascimento), às 2, 4, 6 e 12 horas de vida, a fim de avaliar os resultados de eritrograma, análises bioquímicas e hormonais, comparando-os entre os grupos e momentos. Nos animais que vieram a óbito foi realizada a necropsia para investigar a causa mortis. As diferenças observadas foram em relação aos achados clínico-patológicos, envolvendo, principalmente, o sistema respiratório caracterizado por bradpneia associada à dispneia, e a presença de edema e atelectasia pulmonar observadas no GI. Ademais, após a colostragem notou-se que 80% dos animais avaliados não foram capazes de manter a glicemia sendo mais evidentes nos animais do GII, possivelmente devido à hiperinsulinemia que se manifestou neste grupo ao longo de todo o período experimental. A anemia após o nascimento foi evidente no grupo de bezerros clonados ao longo de todo período de avaliação ao contrário dos bezerros oriundos de GII, a qual foi observada somente às 12 horas de vida, sendo, possivelmente, de origem ferropriva. Casos de hipercalcemia foram denotados nos animais do GI ao nascimento, sendo possivelmente associados à asfixia perinatal. Estas alterações, em conjunto, levaram a uma taxa de mortalidade de 90% dos animais do GI e de 20% dos bezerros do GII. Conclui-se que os animais oriundos da TNCS apresentam alterações fisiológicas e metabólicas após o nascimento, responsáveis, em grande parte, pela maior taxa de óbitos dentro do período perinatal.

5.
Animals (Basel) ; 10(12)2020 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-33287159

RESUMO

The neonatal period is a challenging phase for calves, and during this phase constant adaptations are required. The aim of the present study was to evaluate the invasive hemodynamics with the Swan-Ganz catheter in neonate calves to understand adaptive changes during the first 30 days of life. A prospective and observational study was conducted with 10 Holstein calves. Assessments of the right atrial pressure (RAP), right ventricular pressure (RVP), pulmonary artery pressure (PAP), pulmonary capillary pressure (PW), cardiac output (CO), heart rate (HR), pulmonary vascular resistance (PVR), and blood gas levels were performed. The analyses of PAP, PVR, PW, HR, sO2, and arterial blood gases differed (p < 0.05) between the evaluated periods. Our results indicated transient pulmonary artery hypertension during the process of extrauterine adaptation during the first 30 days of life. This hypertension must be considered as physiological and consequent to the neonatal adaptation process.

6.
Reprod Domest Anim ; 55(11): 1599-1606, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32885881

RESUMO

The aim of the present study was to evaluate the biochemical profile and reproductive performance in postpartum dairy cows with clinical metritis. The research was carried out using performed on 213 pluriparous lactating cows from two dairy herds in southeast Brazil. Dairy cows were assigned into a group of healthy cows (n = 147) and cows with metritis (n = 66). Blood samples to assess serum albumin, cholesterol, fibrinogen, calcium, gamma-glutammyltransferase (GGT), aspartate aminotransferase (AST) and ß-hydroxybutyrate (BHBA) concentrations were performed on day 8 ± 2 postpartum. Metritic cows had lower (p < .05) serum albumin, cholesterol and calcium concentration, and higher (p < .05) GGT and AST activity, and BHBA and fibrinogen concentrations when compared to the healthy group. The calving-to-conception interval and services per pregnancy were higher in metritic cows (p < .01) than in healthy cows and the conception rate at first service was lower in metric group (p < .01). Variable importance in projection plots demonstrated that cholesterol was the main discriminator between metritic and healthy cows. Our results indicated alterations in the biochemical profile and impaired reproduction performance in metritic cows.


Assuntos
Doenças dos Bovinos/sangue , Colesterol/sangue , Endometrite/veterinária , Fertilidade , Animais , Brasil , Bovinos , Indústria de Laticínios , Feminino , Período Pós-Parto/metabolismo , Gravidez , Estudos Prospectivos
7.
Reprod Domest Anim ; 55(9): 1219-1228, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32634252

RESUMO

The aims of this study were to characterize the biochemical profile, the reproductive performance and to identify potential predictive biomarkers of disease state of dairy cows with hyperketonemia, lipomobilization and hypocalcemia raised in tropical conditions in southeastern Brazil. Dairy cows (n = 50) were divided into a group of healthy cows (n = 14), cows with lipomobilization (n = 14), cows with hypocalcemia (n = 11), and a group of cows with hyperketonemia (n = 11). Evaluation of body condition score (BCS), body weight (BW) and blood samples was performed on 21, 14, 7, 4 and 2 days before calving, parturition, 1, 7, 14, 21, 30, 45 and 60 days post-partum and milk production was recorded on days 7, 14, 21, 30, 45 and 60 after parturition. Blood samples were assayed for aspartate aminotransferase (AST), gamma-glutammyltransferase (GGT), albumin, total protein, globulin, fibrinogen, total cholesterol, triglyceride, urea and creatinine concentrations. The biochemical profile, BCS, BW, milk production and reproductive performance differed (p < .05) among the groups. Our findings indicate changes in the biochemical profile of dairy cows with metabolic diseases and impaired production and fertility of dairy cows in this group. Variable importance in projection plots demonstrated that cholesterol, urea, total protein, albumin and fibrinogen in the serum were the strongest discriminators between cows with hypocalcemia and healthy cows; and AST, cholesterol, urea and triglycerides for cows with hyperketonemia and healthy cows; and cholesterol, urea, triglycerides, total protein and fibrinogen for lipomobilization and healthy cows, which might be useful as predictive biomarkers of the disease state.


Assuntos
Biomarcadores/sangue , Doenças dos Bovinos/metabolismo , Doenças Metabólicas/veterinária , Animais , Peso Corporal , Brasil , Bovinos , Doenças dos Bovinos/sangue , Indústria de Laticínios , Feminino , Fertilidade , Hipocalcemia/veterinária , Cetose/veterinária , Lactação , Transtornos do Metabolismo dos Lipídeos/veterinária , Doenças Metabólicas/sangue , Parto , Período Pós-Parto , Gravidez , Clima Tropical
8.
Trop Anim Health Prod ; 51(4): 767-773, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30456689

RESUMO

The objective of this study was to evaluate the effects of different levels of NDF in high-concentrate diets on performance and aspects ruminal, blood, and carcass parameters of finishing lambs. Twenty-four lambs, with initial BW of 24 ± 3 kg, were randomly divided into individual pens, in a completely randomized design, comprising three treatments with eight repetitions each. The treatments consisted of dietary levels of 15%, 20%, and 25% of NDF, based on DM. The diets were composed of corn, soybean meal, minerals, and corn silage and were offered twice a day, with daily control of what was offered and refused, to determine dry matter intake (DMI). The animals were weighed weekly and slaughtered after 50 days of confinement. Performance evaluations, blood parameters, carcass, and ruminal parameters were evaluated. Lambs fed with lower dietary fiber had better carcass yield (P < 0.005). Blood count, blood biochemical parameters, rumen short-chain fatty acid production and protozoan population were not affected by the dietary NDF level. It was concluded that in high-concentrate diets with 15% NDF resulting in better weight gain of the animals in the confinement, good carcass yields without the animal having altered health and ruminal parameters.


Assuntos
Dieta/veterinária , Fibras na Dieta/administração & dosagem , Ácidos Graxos/sangue , Carne , Rúmen/fisiologia , Ovinos/fisiologia , Ganho de Peso , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Animais Recém-Nascidos/sangue , Animais Recém-Nascidos/fisiologia , Masculino , Distribuição Aleatória , Ovinos/sangue , Silagem/análise
9.
Arq. Inst. Biol ; 84: e0082016, 2017. ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-981752

RESUMO

The citrus pulp can be used as a substitute in ruminant feed reducing costs and maintaining the nutritional quality of food. However, this compound should be used carefully so as not to cause harm to the animals. The present report aims to describe the occurrence of dental erosion, actinomycosis and polioencephalomalacia in sheep raised and kept with a wet low pectin citrus pulp based diet, composing 50% of roughage. Actinomycosis was diagnosed in five animals through clinical and radiographic examinations and microbiological culture, and, after treatment, three animals were cured. Polioencephalomalacia was confirmed in ten animals by clinical diagnostics, in nine out of ten animals by therapeutic diagnosis, and in one animal by post-mortem anatomopathological examination. According to the observed, we recommend caution when large amounts of citrus pulp are used as bulky food.(AU)


A polpa cítrica está entre os produtos que podem ser utilizados como substitutos na alimentação de ruminantes, diminuindo os gastos e mantendo a qualidade nutricional do alimento fornecido aos animais, porém, esses alimentos devem ser utilizados de forma que não tragam malefícios. Assim, o presente relato visa apresentar a ocorrência de erosão dentária, actinomicose e polioencefalomalácia em ovinos criados e mantidos recebendo alimentação à base de polpa cítrica úmida despectinada na concentração de 50% do volumoso. A actinomicose foi diagnosticada em cinco animais por meio de exame clínico, radiográfico e cultivo microbiológico, e após tratamento três animais foram curados. Já a polioencefalomalácia foi confirmada em dez animais pelos sintomas manifestados, eficiência da terapia instituída em nove animais e exame anatomopatológico de um animal que veio a óbito. De acordo com o observado, deve-se ter cuidado ao utilizar grande quantidade de polpa cítrica úmida como volumoso.(AU)


Assuntos
Animais , Erosão Dentária , Ruminantes , Actinomicose , Ovinos , Citrus , Ração Animal
11.
Ciênc. rural ; 44(9): 1651-1657, 09/2014. graf
Artigo em Português | LILACS-Express | LILACS | ID: lil-725376

RESUMO

Com o objetivo de avaliar a influência da retenção de placenta (RP) no proteinograma de fêmeas bovinas da raça Holandesa, de propriedades comerciais, foram utilizadas 129 vacas com RP e 145 vacas com parto e pós-parto fisiológicos e sem nenhum tratamento no período avaliado. As amostras de sangue foram divididas nos momentos: 1odia pós-parto (DPP), 2o-3o, 4o-5o, 6o-7o, 8o-14o, 15o-29o, 30o-59o e 60o-90o DPP. O fracionamento das proteínas foi realizado por eletroforese em fita de acetato de celulose e em gel de poliacrilamida, contendo dodecil sulfato de sódio (SDS-PAGE), nas quais se avaliou o comportamento de 19 bandas proteicas identificadas pelos respectivos pesos moleculares, que variaram entre 23KDa e 187KDa. Não houve influência da RP na proteína sérica total e gamaglobulinas. A albumina sérica permaneceu abaixo dos valores de referência até os 90DPP nos animais com RP. Concluiu-se que vacas Holandesas com RP apresentam um quadro de normoproteinemia com hipoalbuminemia e aumento das frações alfaglobulinas e betaglobulinas até os 90DPP, presença de resposta inflamatória de fase aguda positiva pelo significativo aumento de haptoglobina, ceruloplasmina, glicoproteína ácida, e de fase aguda negativa pela diminuição de albumina na primeira semana pós-parto.


The aim of this study was to evaluate the influence of retained placenta on the proteinogram of Holstein cows from commercial dairy farms. Blood samples were collected from 129 animals with retained placenta (RP) and 145 animals with normal delivery and postpartum period, without any treatment, on following days: 1 st day in milk (DIM), 2 nd -3 th , 4 th -5 th , 6 th -7 th , 8 th -14 th , 15 th -29 th , 30 th -59 th and 60 th -90 th DIM were analyzed. Protein electrophoresis were performed in acetate cellulose and sodium dodecyl sulfate polyacrilamide gel (SDS-PAGE), where 19 protein bands were observed with molecular weights between 23KDa and 187KDa. There was no influence on serum total protein and gamma globulins. Serum albumin remained below the normal reference values up to 90DIM. In conclusion, Holstein cows with RP have normoproteinemia with hypoalbuminemia up to 90DIM, presence of positive acute phase response by increase of haptoglobin, ceruloplasmin, acid glycoprotein, alpha and beta globulins and negative acute phase response by decreased of albumin within the first week postpartum.

12.
Braz. j. vet. res. anim. sci ; 50(4): 286-293, 2013. tab
Artigo em Português | LILACS | ID: lil-707827

RESUMO

Foi realizado o estudo retrospectivo das afecções do sistema locomotor de ruminantes atendidos no Serviço de Clínica de Bovinos e Pequenos Ruminantes da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP), no período de 2000 a 2012. Nesse período, foram atendidos 209 casos de animais com problemas lo- comotores, dos quais 62,7% localizados na região distal dos membros e 37,3% na região proximal. Na espécie bovina, com 121 (57,9%) casos atendidos, o comprometimento da região distal dos membros foi observado em 86 (71,07%) e da região proximal em 35 (28,93%) dos casos, respectivamente. A afecção mais frequente observada em bovinos foi a hiperplasia interdigital com 26,74% (23) dos casos atendidos. Entre as afecções na região proximal, houve maior ocor- rência de fraturas, com 48,6% (17) dos casos. Foram atendidos 88 (42,1%) pequenos ruminantes, apresentando lesões na região distal em 51,1% (45) dos casos e 48,9% (43) com lesões na região proximal. Nessas espécies, as lesões de maior ocorrência nas regiões distais e proximais foram, respectivamente, o foot-rot (60%) e as fraturas (77,4%). As afecções do sistema locomotor dos ruminantes foram pouco frequentes entre os animais atendidos no período estudado. Quanto ao prognóstico, foi bom nos animais acometidos com doenças podais, diferentemente das afecções proximais dos bovinos, principalmente fraturas, que apresentaram prognóstico mau.


This study aimed to perform a retrospective study of ruminants attended at the Clinic for Cattle and Small Ruminants (CBPR) of the Faculty of Veterinary Medicine (FMVZ), University of São Paulo (USP) with locomotor diseases from 2000 to 2012. During this period 209 cases were treated. It was found that cases located in the distal limb and in the proximal region were 62.7% and 33.7%, respectively. In bovines, 121 (57.9%) cases were treated, with 86 (71.07%) cases presented in the distal limb and 35 (28.93%) cases in the proximal region. The most common disease was interdigital hyperplasia with 23 (26.74%) cases treated. Fractures were the most frequent disease related to the proximal regioncorresponding to 17 (48.6%) occurrences. In small ruminants, 88 animals (42.1%) were treated with 45 (51.1%) cases in the distal region and the other 43 (48.9%) in the proximal region. In these species, the foot-rot (60%) and fractures (77.4%) was the most common diseases found in the distal and the proximal region, respectively. The disorders of the locomotor system of ruminants were uncommon in the CBPR. While the affected animals with claw diseases have a good prognosis, disorders affecting the upper limb in cattle, mainly fracture, have a poor prognosis.


Assuntos
Animais , Bovinos , Casco e Garras/anatomia & histologia , Ovinos , Ruminantes
13.
Biosci. j. (Online) ; 28(5): 805-809, sept./oct 2012. tab
Artigo em Português | LILACS | ID: biblio-914322

RESUMO

Com intuito de avaliar a variação dos constituintes do eritrograma em fêmeas bovinas da raça Holandesa no pós-parto, foram colhidas 142 amostras de sangue nos primeiros 90 dias pós-parto. As amostras foram colhidas de vacas clinicamente sadias e não reagentes ao antígeno (GP 51) do vírus da Leucose dos Bovinos, divididas em nove grupos experimentais, de acordo com o momento da colheita. As amostras de sangue foram colhidas em frascos contendo EDTA e realizaram-se as provas: contagem do número de hemácias, determinação do volume globular, dosagem de hemoglobina e o cálculo dos índices hematimétricos (VCM, HCM, CHCM). O eritrograma variou no período pós-parto e estas alterações foram observadas nas primeiras 24h pós-parto. A hemoconcentração observada no parto desapareceu nas primeiras 24 horas de puerpério. Os valores oscilaram entre: hemácias, 5,08 ± 0,60 e 6,11 ± 0,72 hemácias /µL; taxa de hemoglobina, 9,85 ± 1,16 e 11,46 ± 1,40 g/dL; volume globular, 27,9 ± 3,3 e 31,9 ± 3,6 %; VCM, 50,10 ± 5,90 e 55,27 ± 6,88 fL; HCM, 18,19 ± 1,63 e 19,50 ± 2,76 pg; CHCM, 34,83 ± 2,07 e 36,88 ± 1,67 %. Os constituintes do eritrograma permaneceram dentro dos limites de referência das raças de bovinos criadas no Estado de São Paulo.


With the intention to evaluate the erythrogram profile during postpartum period in Holstein cows, 142 blood samples were collected from cows during the first 90 days after parturition. Blood samples were collected from clinically healthy cows that were non-reactive to the antigen of bovine leukosis virus (GP 51). These animals were divided into nine experimental groups based on the time of the postpartum sample collection. The blood samples were collected with EDTA and submitted to the following tests: erythrocyte counts, packed cell volume, hemoglobin concentration and calculated blood indices (MCV, MCH, MCHC). There was influence of the postpartum period on the erythrogram. These alterations were observed during the first 24 h after parturition. The hemoconcentration observed during the parturition disappeared during the first 24h of postpartum. Variation of the values were observed: red blood, 5.08 ± 0.60 and 6.11 ± 0.72 cells /µL; hemoglobin concentration, 9.85 ± 1.6 and 11.46 ± 1,40 g/dL; packed cell volume, 27.9 ± 3.3 and 31.9 ± 3.6 %; MVC, 50.10 ± 5.90 and 55.27 ± 6.88 fL; MHC, 18.19 ± 1.63 and 19.50 ± 2.76 pg; MCHC, 34.83 ± 2.07 and 36.88 ± 1.67 %. The values of the hematological components obtained in this study did not exceed the limits of the reference values, obtained from cows raised within the state of São Paulo.


Assuntos
Bovinos , Período Pós-Parto , Hematologia
14.
Acta cir. bras ; 25(5): 416-422, Sept.-Oct. 2010. ilus
Artigo em Inglês | Sec. Est. Saúde SP, LILACS, Sec. Est. Saúde SP | ID: lil-558727

RESUMO

PURPOSE: Evaluate the bone tissue recovery following transplantation of ovine mesenchymal stem cells (MSC) from bone marrow and human immature dental-pulp stem cells (hIDPSC) in ovine model of induced osteonecrosis of femoral head (ONFH). METHODS: Eight sheep were divided in three experimental groups. First group was composed by four animals with ONFH induced by ethanol through central decompression (CD), for control group without any treatment. The second and third group were compose by two animals, six weeks after ONFH induction received transplantation of heterologous ovine MSC (CD + oMSC), and hIDPSC (CD + hIDPSC), respectively. In both experiments the cells were transplanted without application of any type of immunosupression protocol. RESULTS: Our data indicate that both cell types used in experiments were able to proliferate within injured site providing bone tissue recovery. The histological results obtained from CD+hIDPSC suggested that the bone regeneration in such animals was better than that observed in CD animals. CONCLUSION: Mesenchymal stem cell transplant in induced ovine osteonecrosis of femoral head by central decompression technique is safe, and apparently favors bone regeneration of damaged tissues.


OBJETIVO: Verificar os efeitos das células-tronco mesenquimais da medula óssea de ovinos e da polpa dentária imatura humana em ovinos com osteonecrose induzida, da cabeça do fêmur. MÉTODOS: Oito ovelhas foram distribuídas em três grupos experimentais. O primeiro grupo foi composto por quatro animais com osteonecrose da cabeça do fêmur induzida por etanol através da descompressão central, que não receberam nenhum tratamento. O segundo e o terceiro grupo, cada um composto por dois animais, receberam transplante heterólogo de células tronco mesenquimais de ovinos e polpa dentária imatura humana seis semanas após a indução da osteonecrose da cabeça do fêmur, respectivamente. Em ambos os grupos experimentais as células foram transplantadas sem o uso de drogas imunossupressoras. RESULTADOS: Os achados demonstram que as células-tronco mesenquimais injetadas na cabeça do fêmur se encontravam viáveis após o transplante no novo sítio e proliferaram em pouco tempo. Os dados histológicos sugerem que a regeneração óssea nos animais transplantados com polpa dentária imatura humana foi mais rápida do que nos animais submetidos somente a descompressão central. CONCLUSÃO: O transplante de células tronco mesenquimais na osteonecrose da cabeça do fêmur induzida em ovinos através da técnica de descompressão central é um procedimento seguro, e aparentemente favorece a regeneração óssea de tecidos lesados.


Assuntos
Animais , Humanos , Polpa Dentária/transplante , Necrose da Cabeça do Fêmur/cirurgia , Transplante de Células-Tronco Mesenquimais , Materiais Biocompatíveis/efeitos adversos , Modelos Animais de Doenças , Transplante de Células-Tronco Mesenquimais/efeitos adversos , Distribuição Aleatória , Ovinos , Transplante Heterólogo
15.
Acta Cir Bras ; 25(5): 416-22, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20877951

RESUMO

PURPOSE: Evaluate the bone tissue recovery following transplantation of ovine mesenchymal stem cells (MSC) from bone marrow and human immature dental-pulp stem cells (hIDPSC) in ovine model of induced osteonecrosis of femoral head (ONFH). METHODS: Eight sheep were divided in three experimental groups. First group was composed by four animals with ONFH induced by ethanol through central decompression (CD), for control group without any treatment. The second and third group were compose by two animals, six weeks after ONFH induction received transplantation of heterologous ovine MSC (CD + oMSC), and hIDPSC (CD + hIDPSC), respectively. In both experiments the cells were transplanted without application of any type of immunosupression protocol. RESULTS: Our data indicate that both cell types used in experiments were able to proliferate within injured site providing bone tissue recovery. The histological results obtained from CD+hIDPSC suggested that the bone regeneration in such animals was better than that observed in CD animals. CONCLUSION: Mesenchymal stem cell transplant in induced ovine osteonecrosis of femoral head by central decompression technique is safe, and apparently favors bone regeneration of damaged tissues.


Assuntos
Polpa Dentária/transplante , Necrose da Cabeça do Fêmur/cirurgia , Transplante de Células-Tronco Mesenquimais , Animais , Materiais Biocompatíveis/efeitos adversos , Modelos Animais de Doenças , Humanos , Transplante de Células-Tronco Mesenquimais/efeitos adversos , Distribuição Aleatória , Ovinos , Transplante Heterólogo
17.
Braz. j. vet. res. anim. sci ; 46(5): 355-362, 2009. tab, graf
Artigo em Português | LILACS | ID: lil-538427

RESUMO

Com a finalidade de estabelecer os valores de referência dos teores de proteína, gordura e sólidos totais do leite de bovinos da raça Jersey,criados no Estado de São Paulo, durante o primeiro mês de lactação,bem como avaliar a influência da fase colostral, examinaram-se 418 amostras de leite, provenientes de quartos mamários sadios e sem crescimento bacteriano. O leite foi colhido assepticamente antes da ordenha e os valores de proteína, gordura e sólidos totais foram determinados por radiação infra vermelho. Os teores de proteína na secreção láctea diminuíram abruptamente do 1º para o 2º dia de lactação,enquanto os teores de sólidos totais diminuíram abruptamente nas primeiras 24 horas após o parto. Após oscilaram muito nos primeiros três dias de lactação, observou-se que os valores de gordura diminuíam atingindo os valores mínimos entre o 15º e 30º dia de lactação. Sugere-se nas primeiras 24 horas de lactação (período colostral) a adoção dos seguintes valores de referência: proteína - entre 7,35 e 11,45 g/dL;gordura – 0,98 e 2,00 g/dL; e sólidos totais - entre 12,39 e 19,45 g/dL


With the aim to establish reference values of the protein, fat and total solids of milk from Jersey cows, raised in the State of São Paulo, during the first month of lactation, as well as to evaluate the influence of the colostral phase, 418 milk samples, obtained from healthy mammary glands without bacterial growth, were analized. Milk samples were aseptically collected before milking and protein, fat and total solids determined by infrared radiation. The milk protein level decrease from first to second day of lactation, while milk total solids level decreased during the first 24 hours after parturition. Milk fat level oscillated in the first 3 days of lactation and then decreased to the minimum values between 15-30 days of lactation. We suggest, forthe first 24 hours of lactation (colostral phase) the use of the following reference values: protein- between 7,35 and 11,45 g/dl; fat- between 0,98 and 2,00 g/dl ; total solids – between 12,39 and 19,45 g/dl


Assuntos
Animais , Feminino , Bovinos , Lactação/fisiologia , Leite/química , Proteínas do Leite/análise , Sólidos Totais/análise , Brasil , Lactação/metabolismo , Leite/metabolismo
18.
Braz. j. vet. res. anim. sci ; 46(1): 5-10, 2009. tab
Artigo em Português | LILACS | ID: lil-536949

RESUMO

Com o objetivo de avaliar a influência do puerpério fisiológico e da fase pós-puerperal no lipidograma de vacas sadias foram colhidas 104 amostras de sangue por punção da veia coccígea de fêmeas da raça Holandesa criadas no Estado de São Paulo. O lipidograma foi avaliado pela determinação dos teores séricos de colesterol, triglicérides, ácidos graxos não esterificados, beta-hidroxibutirato, e teores plasmáticos de glicose. Observou-se que os teores séricos de colesterol aumentaram gradativamente, enquanto os teores de ácidos graxos não esterificados diminuíram gradativamente com a evolução do puerpério. Na fase pós-puerperal (mais de 45 dias pós-parto) ocorreu a estabilização dos valores de colesterol e de ácidos graxos não esterificados.


Aiming to assess the influence of physiological puerperium and post puerperal phase on the lipid profile of health cows 104 blood sampleswere collected by coccygeal vein puncture of Holstein cows raised inthe State of São Paulo. Lipid profile assessment was measured byserum levels of cholesterol, triglycerides, nonesterified fatty acids, âhydroxybutyrate and plasma glucose level. With regard to puerperiumand post-puerperal phase influence on the lipid profile, gradual increaseof serum cholesterol level and decrease of nonesterified fatty acidswere observed with puerperal evolution. The values of cholesterol and nonsterified fatty acids were stabilized during post-puerperal phase(greater than 45 day postparturm).


Assuntos
Animais , Ácidos Graxos/análise , Análise Química do Sangue/métodos , Bovinos , Colesterol/análise
19.
Braz. j. vet. res. anim. sci ; 45(6)2008. tab, ilus
Artigo em Português | LILACS | ID: lil-510887

RESUMO

Com a finalidade de estabelecer os valores de referência da contagem de células somáticas do leite de bovinos da raça Jersey, criados no Estado de São Paulo, durante o primeiro mês de lactação, bem como avaliar a influência da fase colostral, examinaram-se 418 amostras de leite, provenientes de quartos mamários sadios e sem crescimento bacteriano. O leite foi colhido assepticamente antes da ordenha e a contagem de células somáticas foi determinada por citometria de fluxo e pelo Teste do CMT. Demonstrou-se a significativa influência do primeiro mês de da lactação sobre a contagem de células. Verificou-se que a transição da secreção de colostro para leite em relação ao número de células somáticas está finalizado a partir do 15º dia de lactação, sendo recomendado a adoção dos seguintes valores de referência: no colostro, obtidos nas primeiras 24 horas de lactação entre 472.405 e 2.003.921 células/ml, no período compreendido do 2º ao 15º dia de lactação valores entre 103.920 e 1.298.361 células/ml e a partir do 15º dia de lactação entre 37.714 e 205.549 células/ml.


With the aim to establish reference values of the number somatic cell of milk from Jersey cows, raised in the State of São Paulo, during the first month of lactation, as well as to evaluate the influence of the colostral phase, 418 milk samples were examined obtained from healthy mammary glands without bacterial growth. Milk samples were collected aseptically before milking and number somatic cell determined by electronic counting and by California Mastitis Test. was demonstrated the significant influence the somatic cells in the first month of lactation. It was found that the transition of the colostrum secretion for milk in respect to somatic cells count have been finished in the 15º day of lactation, being recommended the adoption of the following reference values: in the colostrum, getting in the first of 24 hours of lactation between 472.405 and 2.003.921cells/ml, in the understood period from 2º until 15º day of lactation values between 103.920 and 1.298.361 cells/ml and starting from 15 day of lactation between 37.714 and 205.549 cells/ml.


Assuntos
Animais , Bovinos , Contagem de Células/métodos , Lactação , Leite/efeitos adversos
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