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1.
Kardiologiia ; 59(11): 56-65, 2019 Dec 11.
Artigo em Russo | MEDLINE | ID: mdl-31849300

RESUMO

Blood pressure variability (BPV) is the fluctuations of blood pressure over a certain period of time under the influence of various factors. The issue of increased BPV is of particular clinical importance due to high predictive value of this parameter as a risk factor for fatal and non-fatal cardiovascular, cerebrovascular and renal events. It is proved that in the BPV increasing, the key role is played by impairments in arterial baroreflexes, which, in turn, are mediated by increased vascular stiffness, impact of angiotensin II and the sympathetic nervous system, endothelial dysfunction, nitric oxide deficiency and aging, including the vascular aging. Antihypertensive drugs that targeting largest amount of pathophysiological mechanisms in BPV increasing have a most advantages in correcting excessive pressure fluctuations. In this regard such drugs are perindopril and amlodipine, which can eliminate almost the entire spectrum of increased BPV causes and, therefore, optimally reduce the cardiovascular risk.


Assuntos
Anti-Hipertensivos/uso terapêutico , Hipertensão , Pressão Sanguínea , Determinação da Pressão Arterial , Humanos , Hipertensão/tratamento farmacológico
2.
Kardiologiia ; 59(3): 18-26, 2019 Apr 12.
Artigo em Russo | MEDLINE | ID: mdl-30990137

RESUMO

OBJECTIVE: to investigate the impact of indapamide / perindopril single-pill combination (I / P SPC) on arterial stiffness parameters, blood pressure (BP) level and BP variability (BPV) in middle-aged patients with stage II grade 1-2 essential arterial hypertension (EAH). Materials and methods. We retrospectively formed a group of patients with stage II grade 1-2 EAH who had not previously received regular antihypertensive therapy (AHT) (n=52, mean age 52.9±6.0 years). All patients were treated with I / P SPC and all of them achieved target office BP level (less than 140 / 90 mm Hg). After 12 weeks of follow-up (from the time of reaching the target BP) assessment of AHT effectiveness (general clinical data, ambulatory blood pressure monitoring [ABPM], volume sphygmography, echocardiography), and vascular stiffness evaluation were performed. RESULTS: At the end of follow-up office systolic BP (SBP), diastolic BP (DBP), pulse BP, day-time, night-time and 24­hour SBP and DBP significantly (p<0.001 for all) decreased. According to the ABPM data day-time, nighttime, and 24­hour systolic BPV significantly decreased (p=0.029, p=0.006 and p<0.001, respectively); day-time and 24­hour diastolic BPV also significantly decreased (p=0.001 and p<0.001, respectively). Day-night standard deviation (SDdn) significantly decreased too (p=0.002 and p<0.001, respectively). Volumetric sphygmography showed significant decrease of right cardio-ankle vascular index (CAVI) (from 8.20±1.29 to 7.58±1.44, p=0.001) and of left CAVI (from 8.13±1.40 to 7.46±1.43, p<0.001), as well as reduction of the number o f patients with a right- and / or left-CAVI >9.0 (from 32.7 to 11.5 %, p=0.018). According to assessment of arterial stiffness using the Vasotens24 software package, the arterial stiffness index (ASI) significantly (p<0.001) decreased from 153.5±29.9 to 138.3±20.0 (by -9.2±13.1 %). Transthoracic echocardiography data demonstrated significant decrease (p<0.001) in effective arterial elastance (from 1.82±0.43 to 1.58±0.36 mm Hg; by -11.85±16.29 %) and significant (p<0.001) increase in the arterial compliance - from 1.27±0.34 to 1.54±0.38 mm Hg / ml (+26.95±38.06 %). CONCLUSION: In AHT naive patients 40-65 years old with stage II grade 1-2 EAH therapy with I / P SPC provided effective 24­hour BP control, reduced BPV and improved arterial stiffness parameters.


Assuntos
Hipertensão , Indapamida/uso terapêutico , Perindopril/uso terapêutico , Anti-Hipertensivos , Arginina , Pressão Sanguínea , Monitorização Ambulatorial da Pressão Arterial , Humanos , Hipertensão/tratamento farmacológico , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco
3.
Artigo em Russo | MEDLINE | ID: mdl-30251974

RESUMO

AIM: To study cognitive functions, anxiety and depression levels, 24-hour blood pressure (BP) profile, cerebral blood flow (CBF) perfusion in treatment-naive middle-aged patients with uncomplicated essential arterial hypertension (EAH) depending on the white matter hyperintensities (WMH) burden. MATERIAL AND METHODS: Forty-one hypertensive patients (mean age 46.2±4.6 years) and 41 healthy volunteers (mean age 50.3±6.7 years) were enrolled to the study. All subjects underwent brain MRI (MAGNETOM Skyra 3.0T, T1, T2 FSE, T2 FLAIR, T1 MPRAGE, ASL), Montreal cognitive assessment (MoCa), 10-word learning task, verbal fluency test, trail making test, Stroop color and word test, anxiety and depression assessment with Hamilton rating scales, 24-hour blood pressure monitoring (ABPM). RESULTS: WMH were found in 22 (53.7%) hypertensive patients and in 3 (7.3%) healthy volunteers (p=0.0002). Hypertensive patients had the significantly lower CBF compared to controls (p<0.001). CONCLUSION: WMH were identified in treatment-naive middle-aged patients with uncomplicated mild to moderate EAH. There was an association between WMH and lower CBF in the cortical plate of frontal lobes, SBP variability and worse cognition. Cerebral hypoperfusion can cause cognitive impairment even in the earliest stages of EAH, which increases due to emotional disorders.


Assuntos
Disfunção Cognitiva , Hipertensão , Adulto , Encéfalo/diagnóstico por imagem , Estudos de Casos e Controles , Circulação Cerebrovascular , Cognição , Disfunção Cognitiva/complicações , Disfunção Cognitiva/diagnóstico por imagem , Humanos , Hipertensão/complicações , Imagem por Ressonância Magnética , Pessoa de Meia-Idade
4.
Kardiologiia ; (8): 82-88, 2018 Aug.
Artigo em Russo | MEDLINE | ID: mdl-30131046

RESUMO

In this article we present problems of prognostic value of day-by-day blood pressure (BP) variability, its role in development and progression of damage of target organs in arterial hypertension, and impact on risk of cardiovascular, cerebral-vascular complications, and mortality. We also discuss methodological aspects of assessment of day-by-day BP variability. The article contains literature data on effects of antihypertensive therapy on variability of home BP, and consideration of evidence base of possibilities of the fixed amlodipine/perindopril combination for lowering day-by-day BP variability.


Assuntos
Hipertensão , Anlodipino , Anti-Hipertensivos , Pressão Sanguínea , Combinação de Medicamentos , Humanos , Perindopril , Prognóstico
5.
Kardiologiia ; 57(11): 68-75, 2017 Nov.
Artigo em Russo | MEDLINE | ID: mdl-29276920

RESUMO

The article is devoted to the modern state of the problem of blood pressure variability (BPV). Along with discussion of classification and methods of diagnosis it contains data on prognostic value of visit-to-visit BPV. We have also reviewed effect on BPV of various regimens of antihypertensive therapy and presented evidence base supporting ability of amlodipine/perindopril fixed dose combination to lower visit-to-visit BPV.


Assuntos
Hipertensão , Anlodipino , Anti-Hipertensivos , Pressão Sanguínea , Determinação da Pressão Arterial , Combinação de Medicamentos , Humanos , Perindopril
6.
Kardiologiia ; 57(12): 62-72, 2017 Dec.
Artigo em Russo | MEDLINE | ID: mdl-29466213

RESUMO

We discuss in this article problems of prognostic significance of 24­hour arterial pressure variability (24hAPV), as well as the role of 24hAPV in development and progression of various target organs damage by arterial hypertension. We also present literature data on impact of various regimens of antihypertensive therapy on 24hAPV, and on ability of fixed-dose perindopril/amlodipine to lower 24­hAPV.


Assuntos
Pressão Arterial , Hipertensão , Anlodipino , Anti-Hipertensivos , Combinação de Medicamentos , Humanos , Perindopril , Prognóstico
7.
Ter Arkh ; 88(1): 35-39, 2016.
Artigo em Russo | MEDLINE | ID: mdl-26978607

RESUMO

AIM: To evaluate the efficacy of sotalol depending on the magnitude of changes in adrenal responsiveness and autonomic nervous system tone in patients with paroxysmal atrial fibrillation (AF). SUBJECTS AND METHODS: Twenty-six patients with paroxysmal AF in the presence of coronary heart disease (CHD) and hypertension were examined. Sinus rhythm variability and sympathicotonic and vagotonic disorders were studied in patients with paroxysmal AF before and during sotalol treatment. A commercial Beta-ARM-Agat kit was used to estimate the adrenal responsiveness of erythrocyte membranes (ß-APM), which can judge the body's individual sensitivity to ß-adrenoblockers. RESULTS: Sotalol used in the average therapeutic doses of 160-240 mg did not reduce ejection fraction or increase atrioventricular conduction up to abnormal values. In patients with borderline and mild hypertension, the drug lowered blood pressure statistically significantly (p=0.01) and was well tolerated. The drug increased the sensitivity of ß-adrenoblockers in patients with adrenergic AF. CONCLUSION: The effect of sotalol on the autonomic nervous system manifested in the higher power of a high-frequency spectral component of heart rate variability than in that of a low-frequency one. Long-term sotalol administration significantly reduced ß-APM, increasing the sensitivity of adrenoceptors.


Assuntos
Fibrilação Atrial , Sistema Nervoso Autônomo , Frequência Cardíaca/efeitos dos fármacos , Sotalol , Antagonistas Adrenérgicos beta/administração & dosagem , Antagonistas Adrenérgicos beta/farmacocinética , Idoso , Fibrilação Atrial/complicações , Fibrilação Atrial/tratamento farmacológico , Fibrilação Atrial/metabolismo , Sistema Nervoso Autônomo/metabolismo , Sistema Nervoso Autônomo/fisiopatologia , Doença das Coronárias/complicações , Tolerância a Medicamentos , Membrana Eritrocítica/efeitos dos fármacos , Feminino , Sistema de Condução Cardíaco/efeitos dos fármacos , Humanos , Hipertensão/complicações , Masculino , Pessoa de Meia-Idade , Sotalol/administração & dosagem , Sotalol/farmacocinética , Volume Sistólico/efeitos dos fármacos
8.
Eksp Klin Gastroenterol ; (8): 62-66, 2016.
Artigo em Inglês, Russo | MEDLINE | ID: mdl-29874438

RESUMO

Inflammatory bowel disease (IBD) are systemic diseases with different intestinal and extra-intestinal manifestations (ElM). This fact can determin an examination plan and the disease course and prognosis. Up to 8.4% of children with IBD have ELM like the first symptom of the disease. The range of ElM of iBD in children is differ from that one of adult patients: rarer arthropathy and arthritis, skin and liver diseases, an extremely rare eye disease. The physical development disorder is a typical children's manifestation of the IBD, the "failure of the weight curve" is an early symptom of the IBD debut (82% of examined children), especially ulcerative colitis. The article discusses the clinical features and therapy of the most frequent extraintestinal manifestations of IBD in children: skin, mucous, eye diseases, axial and peripheral arthropathies, primary scierosing cholangitis, physical, sexual development disorders, decreased bone mineral density. Most often, we observed patients with arthritis and primary scierosing cholangitis - up to 12.5% of patients with active disease. A growth failure was observed in 20% of children with IBD debut, and remained in 9.7% children despite adequate anti-inflammatory therapy. Decreased bone mineral density in children with IBD observed in 30% cases (ISCD-recommendations). Extraintestinal manifestations are very important to make the early diagnosis of IBD without any intestinal symptoms. The pediatrician can suspect a systemic diseases course. ElMs adversely impact upon patientsquality of life and some can be life-threatening.


Assuntos
Desenvolvimento do Adolescente , Desenvolvimento Infantil , Insuficiência de Crescimento , Doenças Inflamatórias Intestinais , Adolescente , Criança , Pré-Escolar , Insuficiência de Crescimento/diagnóstico , Insuficiência de Crescimento/etiologia , Insuficiência de Crescimento/patologia , Insuficiência de Crescimento/fisiopatologia , Feminino , Humanos , Lactente , Doenças Inflamatórias Intestinais/complicações , Doenças Inflamatórias Intestinais/diagnóstico , Doenças Inflamatórias Intestinais/patologia , Doenças Inflamatórias Intestinais/fisiopatologia , Masculino
9.
Klin Med (Mosk) ; 91(7): 23-7, 2013.
Artigo em Russo | MEDLINE | ID: mdl-24437165

RESUMO

Individual peculiarities of the receptor apparatus of cardiomyocytes may determine pathological features of heart activity and susceptibility to pharmaceuticals. The possible role of beta-adrenoreceptor polymorphism in the development of cardiac rhythm disturbances is assessed by PCR. Special attention is given to A145G polymorphism of the ADRB1 gene in 127 patients with primary cardiac rhythm disorders. It was shown that AJ45G polymorphism (Ser49Gly) at DNA sites encoding for the amino acid sequence of beta-1 adrenoreceptors can influence the development of sex-specific cardiac rhythm disorders.


Assuntos
Arritmias Cardíacas/genética , Receptores Adrenérgicos beta 1/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Arritmias Cardíacas/classificação , Arritmias Cardíacas/diagnóstico , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo Genético/genética , Fatores Sexuais , Adulto Jovem
10.
Zh Nevrol Psikhiatr Im S S Korsakova ; 111(2 Pt 2): 38-51, 2011.
Artigo em Russo | MEDLINE | ID: mdl-21916156

RESUMO

Based on the own observations, we have summarized the features of symptomatic epilepsy in multiple sclerosis and conducted a clinical analysis of the most diagnostically difficult cases of inflammatory and demyelinating diseases in which epilepsy was the major clinical syndrome. The cases of post-infectious acute disseminated encephalomyelitis, idiopathic cerebral angiitis, Rasmussen's encephalitis are reviewed. Peculiarities of MRI structural brain lesions in these patients are discussed. The use of additional laboratory studies, including a study of cerebrospinal fluid, is considered.


Assuntos
Doenças Desmielinizantes/complicações , Epilepsia/diagnóstico , Epilepsia/etiologia , Doenças Desmielinizantes/patologia , Epilepsia/epidemiologia , Feminino , Humanos , Imagem por Ressonância Magnética , Masculino , Esclerose Múltipla/complicações , Esclerose Múltipla/patologia
11.
Zh Nevrol Psikhiatr Im S S Korsakova ; 110(5 Pt 2): 15-8, 2010.
Artigo em Russo | MEDLINE | ID: mdl-21322142

RESUMO

Eighty patients with heroin addiction, aged from 15 to 20 years (most of them over the age of 16), were examined. The duration of illness was from 6 months to 3 years. The examination was conducted 2-3 weeks after arresting of withdrawal syndrome. In that period, patients had different mental disorders: depressive-dysphoric, neurosis-like, anxiety-hypochondriac, hysteriform and others. The aim of the examination was to find peculiarities of functioning of different cortical regions in the process of appraisal of conscious and unconscious stimuli using the method of visual evoked potentials with P300 recording. Stimuli were words highlighted on the screen--indifferent and potentially significant for a patient (e.g. heroin). The stimulus related to the dominating motivation caused in patients the higher diffuse cortical activation on the unconscious level compared to non-targeted stimuli.


Assuntos
Sintomas Afetivos/fisiopatologia , Encéfalo/fisiopatologia , Emoções , Dependência de Heroína/fisiopatologia , Dependência de Heroína/psicologia , Motivação , Adolescente , Potencial Evocado P300 , Humanos , Síndrome de Abstinência a Substâncias/tratamento farmacológico , Síndrome de Abstinência a Substâncias/fisiopatologia , Adulto Jovem
12.
Klin Lab Diagn ; (12): 43-5, 2007 Dec.
Artigo em Russo | MEDLINE | ID: mdl-18225512

RESUMO

Neutrophilic metabolic activity was studied in 21 children with lymphoblastic leukemia during chemotherapy. After one-year chemotherapy, reduced cell activities of catalase and glutathione reductase were observed in children with septic complications during a follow-up in the presence of lower neutrophilic lactate levels. There was a direct correlation between lactate levels and catalase activity. Since the reduction in the levels of lactate in the neutrophils is attended by their apoptosis, this suggests that there is an association of delayed cell death with a fall in the phagocytic antioxidative defense potential.


Assuntos
Catalase/análise , Glutationa Redutase/análise , Neutrófilos/enzimologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Ácido Láctico/análise , Masculino , Estresse Oxidativo , Fagocitose , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico
13.
Ter Arkh ; 75(8): 59-61, 2003.
Artigo em Russo | MEDLINE | ID: mdl-14520854

RESUMO

AIM: To study iron metabolism in patients on programmed hemodialysis (PH) in oral and intravenous administration of iron drugs; to compare clinical and financial results of using such drugs. MATERIAL AND METHODS: A two-stage trial studied iron metabolism in 158 PH patients on replacement therapy with erythropoetin. They received correction of iron deficiency with oral drugs (stage I) and venofer (stage II). RESULTS: The study of iron metabolism has found its deficiency in 2/3 patients receiving oral iron: absolute (48%) and relative (20%). Administration of venofer led to a 2-fold increase in the number of patients with normal iron metabolism. The target Hb and Ht were achieved in 2.5 times more patients than before venofer treatment. The dose of erythpoetin in such cases was reduced by 40%. Side effects were not observed. The week cost of venofer treatment per patient was lower by 22.5$ than the cost of treatment with oral iron drugs. CONCLUSION: Venofer correction of iron deficiency in PH patients is more effective both clinically and financially than use of oral iron preparations.


Assuntos
Anemia Ferropriva/tratamento farmacológico , Compostos Férricos/uso terapêutico , Diálise Renal/efeitos adversos , Sacarose/uso terapêutico , Administração Oral , Adolescente , Adulto , Idoso , Anemia Ferropriva/etiologia , Custos de Medicamentos , Eritropoetina/administração & dosagem , Eritropoetina/uso terapêutico , Feminino , Compostos Férricos/administração & dosagem , Compostos Férricos/economia , Óxido de Ferro Sacarado , Ácido Glucárico , Hemoglobinas/análise , Humanos , Injeções Intravenosas , Nefropatias/terapia , Masculino , Pessoa de Meia-Idade , Sacarose/administração & dosagem , Sacarose/economia , Resultado do Tratamento
14.
Mikrobiol Z ; 64(3): 75-80, 2002.
Artigo em Russo | MEDLINE | ID: mdl-12190039

RESUMO

Immunosuppressive activity of culture liquid substrate (CFS) of highly virulent strain Salmonella typhimurium has been studied. A model of delayed hypersensitivity (DHS) to nonbacterial antigen in mice, a method of gel-filtration through the sephadex column G-200, immunosorbents were used. Three components with immunosuppressive activity: thermolabile component and thermostable one with direct immunosuppressive action and the third thermolabile component which manifested inductive immunosuppressive activity only after redox treatment have been revealed in the strain CFS. O-specific and lipid parts were found in the composition of all the components. This allowed them to be related to lipopolysaccharide.


Assuntos
Imunossupressores/farmacologia , Lipopolissacarídeos/farmacologia , Salmonella typhimurium/patogenicidade , Animais , Meios de Cultura/química , Modelos Animais de Doenças , Hipersensibilidade Tardia/etiologia , Imunoadsorventes , Imunossupressores/isolamento & purificação , Lipopolissacarídeos/isolamento & purificação , Camundongos , Antígenos O/isolamento & purificação , Antígenos O/farmacologia , Oxirredução , Salmonella typhimurium/imunologia , Temperatura Ambiente , Virulência
15.
Vestn Rentgenol Radiol ; (2): 17-20, 2001.
Artigo em Russo | MEDLINE | ID: mdl-11503173

RESUMO

The authors used computed tomography (CT) as a basis to analyze the anatomic status of the bronchopulmonary system in 28 patients undergone resection of a part of the lung in childhood for chronic nonspecific lung inflammations and malformations. The findings confirmed the high diagnostic value of CT in the evaluation of the lung in different postoperative periods and showed a certain range of changes varying from significant in the lung operated on to progression of the primary disease to the collateral lung.


Assuntos
Pulmão/diagnóstico por imagem , Pneumonectomia , Tomografia Computadorizada por Raios X , Adolescente , Adulto , Fatores Etários , Criança , Seguimentos , Humanos , Lactente , Prognóstico , Fatores de Tempo
16.
Vestn Ross Akad Med Nauk ; (2): 21-5, 2001.
Artigo em Russo | MEDLINE | ID: mdl-11338500

RESUMO

The review deals with the Immunosuppressive effects of virulent gram-negative bacteria (Salmonella, Shigela, Pseudomonas aeruginosa), with the importance of these effects for the bacteria to survive in the infected body. The above bacteria affect the immunity system in a different way, yet have common features. They are characterized by the occurrence of endotoxin shock, by the suppression of the phagocytic system and cell-mediated immunity. A significant role in suppressing a cellular immune response is played by the lipopolysaccharide of virulent bacteria that greatly differs from that of nonvirulent strains. The immunosuppressive activity of the bacteria and their lipopolysaccharide is closely related to their virulent properties.


Assuntos
Bactérias Gram-Negativas/imunologia , Bactérias Gram-Negativas/patogenicidade , Imunidade Celular/imunologia , Lipopolissacarídeos/imunologia , Animais , Bactérias Gram-Negativas/metabolismo , Humanos , Lipopolissacarídeos/metabolismo , Pseudomonas aeruginosa/imunologia , Pseudomonas aeruginosa/metabolismo , Pseudomonas aeruginosa/patogenicidade , Salmonella/imunologia , Salmonella/metabolismo , Salmonella/patogenicidade , Shigella/imunologia , Shigella/metabolismo , Shigella/patogenicidade , Virulência
17.
Artigo em Russo | MEDLINE | ID: mdl-11236501

RESUMO

Immunosuppressive activity of Salmonella typhimurium extracellular lipopolysaccharide (LPS) was studied. In this study isogenic S. typhimurium strains with different degree of virulence were used. The attenuation of these strains was linked with mutations on their chromosome (altered synthesis of RNA polymerase or gyrase DNA) or their own virulence plasmid (the insertion of transposon Tn-5). To obtain LPS fraction with different molecular weights, the filtrate of bacterial culture was subjected to gel filtration through a column packed with Sephadex G-200. The immunosuppressive action of LPS fractions was determined on the model of delayed-type hypersensitivity to nonbacterial antigen in experiments on BALB/c mice. The study revealed that transposon-mediated mutation on plasmid, accompanied by the attenuation of salmonellae, led to the loss of immunosuppressive activity of the high-molecular heat-sensitive component of LPS; only the second heat-resistant component with medium molecular weight retained its activity. The presence of two chromosomal attenuating mutations (rifr nalr) was accompanied by the loss of immunosuppressive activity in both components of LPS.


Assuntos
Lipopolissacarídeos/imunologia , Salmonella typhimurium/patogenicidade , Animais , Camundongos , Camundongos Endogâmicos BALB C , Plasmídeos , Salmonella typhimurium/genética , Virulência/genética
18.
Prikl Biokhim Mikrobiol ; 36(5): 597-602, 2000.
Artigo em Russo | MEDLINE | ID: mdl-11042887

RESUMO

Immunobiological properties of native lipopolysaccharides (LPS) from virulent and avirulent strains of Shigella sonnei bacteria (LPS-V and LPS-A, respectively) were studied. In avirulent bacteria, LPS-V induced immunosuppressive activity specific of the virulent strain. LPS of the avirulent strain, whereas LPS-A lacked this property. Native LPS-V with immunosuppressive activity were isolated from the virulent strain by and immune affinity method. Treatment of LPS-V with phenol or TCA abolished its activity and converted it into the LPS-A form. The data showed that LPS-A can be converted back to the LPS-V form by redox treatment. This approach seems to be promising for activating LPS extracted from cells with TCA or a water-phenol mixture.


Assuntos
Disenteria Bacilar/imunologia , Lipopolissacarídeos/imunologia , Shigella sonnei/imunologia , Animais , Antígenos de Bactérias/imunologia , Disenteria Bacilar/microbiologia , Cobaias , Shigella sonnei/patogenicidade , Virulência/imunologia
19.
Artigo em Russo | MEDLINE | ID: mdl-11210632

RESUMO

The immunosuppressive activity of lipopolysaccharide (LPS) contained in filtrates of newly isolated P. aeruginosa cultures was studied. The experimental model of delayed hypersensitivity to non-bacterial antigen in CBA mice and gel filtration through Sephadex G-200 were used. In addition to the already known LPS component with direct immunosuppressive action and having a mol.wt. of 150-800 kD, a new component was detected. It was found to be in inactive state and could be activated by redox treatment, thus becoming capable of inducing capacity for immunosuppression in Escherichia coli. This component had a mol.wt. of 50-70 kD and lost its activity after heating.


Assuntos
Lipopolissacarídeos/imunologia , Pseudomonas aeruginosa/imunologia , Animais , Cromatografia em Gel , Escherichia coli/efeitos dos fármacos , Escherichia coli/imunologia , Espaço Extracelular/imunologia , Humanos , Hipersensibilidade Tardia/imunologia , Lipopolissacarídeos/química , Lipopolissacarídeos/farmacologia , Camundongos , Camundongos Endogâmicos CBA , Peso Molecular , Oxirredução , Pseudomonas aeruginosa/isolamento & purificação
20.
Mikrobiol Z ; 61(4): 64-71, 1999.
Artigo em Russo | MEDLINE | ID: mdl-10565153

RESUMO

The antibody formation and expression of delayed type hypersensitivity (DTH) of several antigens under the conditions of their injection together with bacterial lipopolysaccharide (LPS) in mice have been studied. Water-phenol LPS extract from S. sonnei was used. The LPS was treated with the redox system (LPS-R) or not (LPS-PW). Besides, LPS was obtained by immunoaffinity method from avirulent (LPS-A) or virulent (LPS-V) S. sonnei. It had been found that the mice reacted by more intensive increase antibody formation to LPS or sheep erythrocytes if LPS-R or LPS-V were injected alone or together with erythrocytes. More intensive increase of lymph nodes was shown as well. When specified LPS were injected in mice together with E. coli they induced the immunosuppression of DTH. The LPS-PW and LPS-A caused the most feeble reaction of immune system in mice. It has been suggested that the differences of immune reaction are the reflection of conformation processes in LPS molecules.


Assuntos
Lipopolissacarídeos/imunologia , Shigella sonnei/imunologia , Animais , Anticorpos Antibacterianos/sangue , Especificidade de Anticorpos/imunologia , Escherichia coli/imunologia , Hipersensibilidade Tardia/imunologia , Imunidade Celular/imunologia , Imunização/métodos , Lipopolissacarídeos/administração & dosagem , Linfonodos/imunologia , Linfonodos/patologia , Camundongos , Shigella sonnei/efeitos dos fármacos , Shigella sonnei/patogenicidade , Virulência/imunologia
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