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1.
FEBS J ; 288(16): 4728-4729, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34398531

RESUMO

With the current issue of The FEBS Journal, we are introducing a new category of invited review article contributions on Emerging Methods and Technologies. These articles provide an overview and discussion of recent, emerging methods that significantly advance and improve research efforts in the different fields of molecular and cellular research of our The FEBS Journal authors and readers. Deputy Editorial Manager Manuel Breuer and our Emerging Methods and Technologies Commissioning Editor Eric Chevet introduce the series.


Assuntos
Doenças Autoimunes/imunologia , Proteínas de Membrana/metabolismo , Neoplasias/imunologia , Polietilenotereftalatos/metabolismo , Polissacarídeos/imunologia , Humanos , Proteínas de Membrana/análise , Neoplasias/terapia , Polissacarídeos/química
2.
FEBS J ; 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34263998

RESUMO

Every quarter, The FEBS Journal presents some of its "hidden gems"-original research and review-type articles that provide a significant advance or discuss recent developments in the molecular or cellular life sciences. These articles are of high value to the scientific community, and we like to take the opportunity to promote these contributions from previous issues of the journal, as we feel their scientific content merits a boost in exposure.

3.
FEBS J ; 288(14): 4165-4167, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33788396

RESUMO

Every quarter, The FEBS Journal presents some of its 'hidden gems' - original research and review-type articles that provide a significant advance or discuss recent developments in the molecular or cellular life sciences. These articles are of high value to the scientific community, and we like to take the opportunity to promote these contributions from previous issues of the journal, as we feel their scientific content merits a boost in exposure.


Assuntos
Pesquisa Biomédica , Publicações Periódicas como Assunto/estatística & dados numéricos , Editoração/estatística & dados numéricos , Humanos
4.
FEBS J ; 287(17): 3612-3632, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32500928

RESUMO

The newly recognised coronavirus SARS-CoV-2, causative agent of coronavirus disease (COVID-19), has caused a pandemic with huge ramifications for human interactions around the globe. As expected, research efforts to understand the virus and curtail the disease are moving at a frantic pace alongside the spread of rumours, speculations and falsehoods. In this article, we aim to clarify the current scientific view behind several claims or controversies related to COVID-19. Starting with the origin of the virus, we then discuss the effect of ibuprofen and nicotine on the severity of the disease. We highlight the knowledge on fomites and SARS-CoV-2 and discuss the evidence and explications for a disproportionately stronger impact of COVID-19 on ethnic minorities, including a potential protective role for vitamin D. We further review what is known about the effects of SARS-CoV-2 infection in children, including their role in transmission of the disease, and conclude with the science on different mortality rates between different countries and whether this hints at the existence of more pathogenic cohorts of SARS-CoV-2.


Assuntos
COVID-19/epidemiologia , COVID-19/transmissão , Pandemias , SARS-CoV-2/patogenicidade , Deficiência de Vitamina D/epidemiologia , Adulto , Idoso , COVID-19/etnologia , COVID-19/patologia , Criança , Grupos de Populações Continentais , Medicina Baseada em Evidências , Feminino , Fômites/virologia , Humanos , Ibuprofeno/administração & dosagem , Ibuprofeno/efeitos adversos , Masculino , Nicotina/efeitos adversos , Preconceito/psicologia , Índice de Gravidade de Doença , Deficiência de Vitamina D/etnologia , Deficiência de Vitamina D/patologia
5.
PLoS Genet ; 12(8): e1006241, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27494704

RESUMO

During prophase of the first meiotic division (prophase I), chromatin dynamically reorganises to recombine and prepare for chromosome segregation. Histone modifying enzymes are major regulators of chromatin structure, but our knowledge of their roles in prophase I is still limited. Here we report on crucial roles of Kdm5/Lid, one of two histone demethylases in Drosophila that remove one of the trimethyl groups at Lys4 of Histone 3 (H3K4me3). In the absence of Kdm5/Lid, the synaptonemal complex was only partially formed and failed to be maintained along chromosome arms, while localisation of its components at centromeres was unaffected. Kdm5/Lid was also required for karyosome formation and homologous centromere pairing in prophase I. Although loss of Kdm5/Lid dramatically increased the level of H3K4me3 in oocytes, catalytically inactive Kdm5/Lid can rescue the above cytological defects. Therefore Kdm5/Lid controls chromatin architecture in meiotic prophase I oocytes independently of its demethylase activity.


Assuntos
Cromossomos/genética , Proteínas de Drosophila/genética , Histona Desmetilases/biossíntese , Meiose/genética , Animais , Centrômero/genética , Segregação de Cromossomos/genética , Metilação de DNA/genética , Drosophila/genética , Proteínas de Drosophila/biossíntese , Histona Desmetilases/genética , Prófase Meiótica I/genética , Oócitos/crescimento & desenvolvimento , Oócitos/metabolismo , Complexo Sinaptonêmico/genética
6.
Genes Dev ; 29(17): 1789-94, 2015 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-26341556

RESUMO

The nuclear pore complex (NPC) tethers chromatin to create an environment for gene regulation, but little is known about how this activity is regulated to avoid excessive tethering of the genome. Here we propose a negative regulatory loop within the NPC controlling the chromatin attachment state, in which Nup155 and Nup93 recruit Nup62 to suppress chromatin tethering by Nup155. Depletion of Nup62 severely disrupts chromatin distribution in the nuclei of female germlines and somatic cells, which can be reversed by codepleting Nup155. Thus, this universal regulatory system within the NPC is crucial to control large-scale chromatin organization in the nucleus.


Assuntos
Cromatina/química , Drosophila melanogaster/genética , Poro Nuclear/química , Animais , Linhagem Celular , Cromatina/genética , Cromatina/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Feminino , Deleção de Genes , Meiose , Membrana Nuclear/metabolismo , Poro Nuclear/genética , Poro Nuclear/metabolismo , Complexo de Proteínas Formadoras de Poros Nucleares/genética , Complexo de Proteínas Formadoras de Poros Nucleares/metabolismo , Estrutura Secundária de Proteína/genética
7.
J Cell Biol ; 191(7): 1251-60, 2010 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-21173113

RESUMO

In contrast to somatic cells, formation of acentriolar meiotic spindles relies on the organization of microtubules (MTs) and MT-organizing centers (MTOCs) into a stable bipolar structure. The underlying mechanisms are still unknown. We show that this process is impaired in hepatoma up-regulated protein (Hurp) knockout mice, which are viable but female sterile, showing defective oocyte divisions. HURP accumulates on interpolar MTs in the vicinity of chromosomes via Kinesin-5 activity. By promoting MT stability in the spindle central domain, HURP allows efficient MTOC sorting into distinct poles, providing bipolarity establishment and maintenance. Our results support a new model for meiotic spindle assembly in which HURP ensures assembly of a central MT array, which serves as a scaffold for the genesis of a robust bipolar structure supporting efficient chromosome congression. Furthermore, HURP is also required for the clustering of extra centrosomes before division, arguing for a shared molecular requirement of MTOC sorting in mammalian meiosis and cancer cell division.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Centrossomo/metabolismo , Meiose/fisiologia , Centro Organizador dos Microtúbulos/metabolismo , Mitose/fisiologia , Neoplasias/patologia , Fuso Acromático/metabolismo , Anáfase/genética , Animais , Proteínas de Ciclo Celular/genética , Linhagem Celular Tumoral , Segregação de Cromossomos/genética , Feminino , Expressão Gênica/genética , Humanos , Cinesina/antagonistas & inibidores , Cinesina/metabolismo , Cinetocoros/metabolismo , Camundongos , Camundongos da Linhagem 129 , Camundongos Knockout , Proteínas Associadas aos Microtúbulos/metabolismo , Microtúbulos/metabolismo , Neoplasias/genética , Oócitos/metabolismo , RNA Interferente Pequeno/genética , Fuso Acromático/genética
8.
PLoS Genet ; 6(10): e1001179, 2010 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-21060809

RESUMO

The meiotic recombination checkpoint is a signalling pathway that blocks meiotic progression when the repair of DNA breaks formed during recombination is delayed. In comparison to the signalling pathway itself, however, the molecular targets of the checkpoint that control meiotic progression are not well understood in metazoans. In Drosophila, activation of the meiotic checkpoint is known to prevent formation of the karyosome, a meiosis-specific organisation of chromosomes, but the molecular pathway by which this occurs remains to be identified. Here we show that the conserved kinase NHK-1 (Drosophila Vrk-1) is a crucial meiotic regulator controlled by the meiotic checkpoint. An nhk-1 mutation, whilst resulting in karyosome defects, does so independent of meiotic checkpoint activation. Rather, we find unrepaired DNA breaks formed during recombination suppress NHK-1 activity (inferred from the phosphorylation level of one of its substrates) through the meiotic checkpoint. Additionally DNA breaks induced by X-rays in cultured cells also suppress NHK-1 kinase activity. Unrepaired DNA breaks in oocytes also delay other NHK-1 dependent nuclear events, such as synaptonemal complex disassembly and condensin loading onto chromosomes. Therefore we propose that NHK-1 is a crucial regulator of meiosis and that the meiotic checkpoint suppresses NHK-1 activity to prevent oocyte nuclear reorganisation until DNA breaks are repaired.


Assuntos
Núcleo Celular/metabolismo , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Meiose/genética , Protamina Quinase/genética , Animais , Linhagem Celular , Polaridade Celular , Quebras de DNA de Cadeia Dupla/efeitos da radiação , Dano ao DNA , Reparo do DNA/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/citologia , Drosophila melanogaster/metabolismo , Feminino , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Histonas/metabolismo , Immunoblotting , Masculino , Modelos Biológicos , Mutação , Oócitos/metabolismo , Fosforilação , Protamina Quinase/metabolismo
9.
J Cell Sci ; 122(Pt 4): 535-45, 2009 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-19174464

RESUMO

The formation of the mitotic spindle is controlled by the microtubule organizing activity of the centrosomes and by the effects of chromatin-associated Ran-GTP on the activities of spindle assembly factors. In this study we show that Mars, a Drosophila protein with sequence similarity to vertebrate hepatoma upregulated protein (HURP), is required for the attachment of the centrosome to the mitotic spindle. More than 80% of embryos derived from mars mutant females do not develop properly due to severe mitotic defects during the rapid nuclear divisions in early embryogenesis. Centrosomes frequently detach from spindles and from the nuclear envelope and nucleate astral microtubules in ectopic positions. Consistent with its function in spindle organization, Mars localizes to nuclei in interphase and associates with the mitotic spindle, in particular with the spindle poles, during mitosis. We propose that Mars is an important linker between the spindle and the centrosomes that is required for proper spindle organization during the rapid mitotic cycles in early embryogenesis.


Assuntos
Divisão do Núcleo Celular , Centrossomo/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Fuso Acromático/metabolismo , Animais , Aurora Quinases , Blastoderma/citologia , Blastoderma/metabolismo , Proteínas de Ciclo Celular , Linhagem Celular , Centrossomo/ultraestrutura , Demecolcina/farmacologia , Proteínas de Drosophila/deficiência , Drosophila melanogaster , Dineínas/deficiência , Feminino , Masculino , Microscopia Confocal , Microtúbulos/efeitos dos fármacos , Microtúbulos/metabolismo , Mutagênese , Proteínas do Tecido Nervoso/química , Proteínas do Tecido Nervoso/genética , Estrutura Terciária de Proteína/fisiologia , Proteínas Serina-Treonina Quinases/deficiência , Proteínas Associadas SAP90-PSD95 , Fuso Acromático/ultraestrutura , Moduladores de Tubulina/farmacologia
10.
Mol Cell Endocrinol ; 282(1-2): 70-7, 2008 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-18155829

RESUMO

The first 4 days of mouse pre-implantation development are characterized by a period of segmentation, including morphogenetic events that are required for the divergence of embryonic and extra-embryonic lineages. These extra-embryonic tissues are essential for the implantation into the maternal uterus and for the development of the foetus. In this review, we first discuss data showing unambiguously that no essential axis of development is set up before the late blastocyst stage, and explain why the pre-patterning described during the early phases (segmentation) of development in other vertebrates cannot apply to mammalian pre-implantation period. Then, we describe important cellular and molecular events that are required for the morphogenesis of the blastocyst.


Assuntos
Blastocisto/fisiologia , Desenvolvimento Embrionário/fisiologia , Morfogênese/fisiologia , Animais , Blastômeros/fisiologia , Diferenciação Celular/fisiologia , Divisão Celular/fisiologia , Embrião de Mamíferos/fisiologia , Camundongos
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