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2.
Am J Orthod Dentofacial Orthop ; 159(4): e343-e362, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33653640

RESUMO

INTRODUCTION: Research on clear aligner treatment (CAT) has increased in recent years. In this study, we aimed to perform a bibliometric and visualized analysis to identify and critically assess the 50 most highly cited articles on CAT. METHODS: Web of Science was selected as a data source and consulted until March 2020 to identify all articles potentially relevant to the analysis. All the eligible articles were collected until 50 manuscripts were listed. Article-based parameters, journal-based parameters, and author-based parameters were registered to perform the bibliometric analysis. Keywords were automatically harvested from the selected articles to implement the visualized analysis. RESULTS: The search identified a total of 378 articles; the total number of citations of the selected articles varied from 15 to 112. The average number of citations per year varied from 1.15 to 13.83. The predominant study design was clinical (31.7%). Over the 15 journals in which the most cited articles were published, the American Journal of Orthodontics and Dentofacial Orthopedics published the majority of those included in the list (14) and also received the greatest number of citations (671). A total of 195 authors contributed to the 50 most cited articles; a significant portion of them (26) were unaffiliated with academic institutions. A total of 184 keywords were gathered from the article list. CONCLUSIONS: The number of citations on CAT is expected to grow steadily in parallel with the rising number of research projects. The present work identifies the most influential articles on CAT and their characteristics, placing emphasis on the journals, the authors, and the topics addressed.


Assuntos
Aparelhos Ortodônticos Removíveis , Ortopedia , Bibliometria
3.
Materials (Basel) ; 12(14)2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-31295822

RESUMO

OBJECTIVE: To perform a systematic review (SR) of existing literature and a patent landscape report (PLR) regarding the potential applications of shape-memory polymers (SMPs) in dentistry. SEARCH STRATEGY: Clinical and Biomedical online databases (Pubmed, Medline via Embase, Scopus, LILACS, Web of Science, Cochrane Library), Materials Science and Engineering databases (IEEE Explore, Compendex, Proquest), Material Science and Chemical database (Reaxys) so as Patents databases (Questel-Orbit, Espacenet, Patentscope) were consulted as recently as January 2019 to identify all papers and patents potentially relevant to the review. The reference lists of all eligible studies were hand searched for additional published work. RESULTS: After duplicate selection and extraction procedures, 6 relevant full-text articles from the initial 302 and 45 relevant patents from 497 were selected. A modified Consolidated Standards of Reporting Trials (CONSORT) checklist of 14 items for reporting pre-clinical in-vitro studies was used to rate the methodological quality of the selected papers. The overall quality was judged low. CONCLUSIONS: Despite the great potential and versatility of SMPs, it was not possible to draw evidence-based conclusions supporting their immediate employment in clinical dentistry. This was due to the weak design and a limited number of studies included within this review and reflects the fact that additional research is mandatory to determine whether or not the use of SMPs in dentistry could be effective. Nevertheless, the qualitative analysis of selected papers and patents indicate that SMPs are promising materials in dentistry because of their programmable physical properties. These findings suggest the importance of furtherly pursuing this line of research.

4.
Cornea ; 32(1): 70-5, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23023402

RESUMO

PURPOSE: The demands for precut lamellar grafts for Descemet stripping automated endothelial keratoplasty rose in our eye bank from 74 in 2007 to 408 in 2010. To meet this expanding requirement, we explored the possibility to preserve these preparations in organ culture. METHODS: Organ cultured corneas, stored in a medium containing 6% dextran, were mounted on a Moria artificial anterior chamber, deprived of the epithelium and then cut with a microkeratome. The posterior lamella was protected by positioning the anterior stromal cap, trephined at a diameter of 8.5 mm and stored at 31°C in the medium containing dextran. The endothelium was examined with trypan blue and alizarin staining and tested for its glycolytic activity (conversion of glucose into lactate). RESULTS: Incubation for a period of 1 week caused a small increase in the cell loss observed after trephination (from 6.2% to 10.6%). After 2 weeks, the decrease in endothelial cell density was 19.9% but the endothelial organization remained intact. The rate of glycolysis remained unchanged during the 2 weeks of preservation, with the majority of glucose uptake accounted for by lactate production. The thickness of the lenticules remained constant, ranging from 170 to 180 µm during the preservation. CONCLUSIONS: The lamellar grafts for Descemet stripping automated endothelial keratoplasty may be stored in organ culture for 2 weeks without damaging the endothelium or increasing the overall thickness.


Assuntos
Córnea , Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior , Bancos de Olhos/métodos , Preservação de Órgãos/métodos , Doadores de Tecidos , Adulto , Idoso , Contagem de Células , Pré-Escolar , Endotélio Corneano/citologia , Endotélio Corneano/fisiologia , Glucose/metabolismo , Glicólise/fisiologia , Humanos , Pessoa de Meia-Idade , Técnicas de Cultura de Órgãos/métodos
5.
Chem Biodivers ; 8(4): 624-42, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21480508

RESUMO

Chemical fingerprinting of commercial Pelargonium capitatum (Geraniaceae) essential oil samples of south African origin was performed by GC, GC/MS, and (13) C- and (1) H-NMR. Thirty-seven compounds were identified, among which citronellol (32.71%) and geraniol (19.58%) were the most abundant. NMR Spectra of characteristic chemicals were provided. Broad-spectrum bioactivity properties of the oil were evaluated and compared with those of commercial Thymus vulgaris essential oil with the aim to obtain a functional profile in terms of efficacy and safety. P. capitatum essential oil provides a good performance as antimicrobial, with particular efficacy against Candida albicans strains. Antifungal activity performed against dermatophyte and phytopathogen strains revealed the latter as more sensitive, while antibacterial activity was not remarkable against both Gram-positive and Gram-negative bacteria. P. capitatum oil provided a lower antioxidant activity (IC(50) ) than that expressed by thyme essential oil, both in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ß-carotene bleaching tests. Results in photochemiluminescence (PCL) assay were negligible. To test the safety aspects of P. capitatum essential oil, mutagenic and toxicity properties were assayed by Ames test, with and without metabolic activation. Possible efficacy of P. capitatum essential oil as mutagenic protective agent against NaN(3) , 2-nitrofluorene, and 2-aminoanthracene was also assayed, providing interesting and significant antigenotoxic properties.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Pelargonium/química , Antibacterianos/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Infecções Bacterianas/tratamento farmacológico , Fungos/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Mutagênicos/química , Mutagênicos/isolamento & purificação , Mutagênicos/farmacologia , Micoses/tratamento farmacológico , Óleos Voláteis/isolamento & purificação
6.
Cornea ; 24(5): 603-7, 2005 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15968169

RESUMO

PURPOSE: This study was designed to define the risk of contamination of human corneas preserved by the organ-culture method. METHODS: We examined the microbial contaminations in 3,100 corneoscleral rims cultivated in our eye bank. Microbiologic tests were performed in the preservation medium 5 days after the beginning of cornea cultures and in the last day of culture (21.5 +/- 8.1 days), when the corneas were transferred to the deswelling medium. In 1,029 corneas a microbiologic test also was performed 1 day after the beginning of deswelling procedure. RESULTS: We found 206 microbial contaminations (6.65% of total) after 5 days and 17 (0.55%) at the end of the preservation period. The total number of contaminated samples during the cornea culture was 223 corresponding to 7.2% of the samples (95% confidence interval, 6.3-8.1). The 1,029 tests performed during the deswelling step disclosed 26 contaminated cornea cultures despite apparent sterility of the medium (2.5%; 95% confidence interval, 1.5-3.5). CONCLUSIONS: The observation of microbial contaminations in a time close to the transplant (i.e., at the end of the preservation period and in the deswelling step) showed that a fast microbial tests during the deswelling procedure may prevent the grafting of a contaminated cornea. The appearance of bacteria in the deswelling medium despite a negative culture medium suggests that bacteria penetrate the corneal tissues during the culture to be subsequently extruded when the internal fluids move outward.


Assuntos
Bactérias/isolamento & purificação , Córnea/microbiologia , Doadores de Tecidos , Fenômenos Fisiológicos Bacterianos , Criopreservação , Meios de Cultura , Bancos de Olhos , Humanos , Técnicas de Cultura de Órgãos , Preservação de Órgãos , Esclera/microbiologia
7.
Ophthalmic Res ; 35(6): 313-8, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14688420

RESUMO

PURPOSE: To improve the preparation of lenticules from human cornea and to obtain their preservation without loss of viable keratocytes. METHODS: The epithelium was manually removed after bathing the surface of the cornea with a solution of trypsin and EDTA. Lenticules were prepared by microkeratome resection and viable keratocytes were visualized by staining with thiazolyl blue (MTT). RESULTS: The pretreatment with trypsin-EDTA allowed the removal of the epithelium without damage to the keratocytes and the stroma. When these lenticules were incubated in Optisol-GS for 7 days at 4 degrees C, they showed a limited thickness increase and a preservation of keratocyte viability. CONCLUSION: This procedure allows the preparation of lenticules with viable keratocytes that can be preserved in the cold for at least 1 week.


Assuntos
Córnea , Substância Própria/citologia , Criopreservação/métodos , Fibroblastos/citologia , Preservação de Órgãos/métodos , Sobrevivência Celular/fisiologia , Sulfatos de Condroitina , Misturas Complexas , Crioprotetores/uso terapêutico , Meios de Cultura Livres de Soro , Desbridamento , Dextranos , Gentamicinas , Humanos , Ceratoplastia Penetrante , Coloração e Rotulagem/métodos
8.
Cornea ; 22(3): 254-8, 2003 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12658094

RESUMO

PURPOSE: To explore the possibility of replacing bovine serum with chicken ovalbumin in cornea preservation. METHODS: Twenty-one pairs of corneas were cultivated at 31 degrees C in a medium containing either 2% (vol/vol) of newborn calf serum (one cornea) or 2 mg/mL of chicken ovalbumin (the contralateral cornea). The evaluation of corneal endothelium was performed after 7 days (one pair) and between 11 and 37 days (20 pairs). Evaluation included the number and the morphology of endothelial cells, their viability, and the pattern of swelling of the intercellular borders. RESULTS: A preliminary test showed that the incubation of a cornea for 7 days with ovalbumin did not cause acute toxic effects. Subsequent experiments showed that this protein was as efficient as bovine serum in preserving the viability and the density of endothelial cells for long periods of incubation. After a mean of 21 +/- 10 days of culture, the number of endothelial cells was 2,455 +/- 230/mm2 in the serum group and 2,430 +/- 181/mm2 in the ovalbumin group. During the longest incubation period, irregular swelling of cell borders and other signs of endothelial degeneration were occasionally observed. CONCLUSION: Replacing serum with chicken ovalbumin resulted in the efficient preservation of corneal endothelium for at least 3 weeks of culture. These data suggest that ovalbumin may be an effective basic component in the design of a serum-free culture medium.


Assuntos
Sangue , Córnea , Meios de Cultura Livres de Soro/farmacologia , Endotélio Corneano/efeitos dos fármacos , Preservação de Órgãos/métodos , Ovalbumina/farmacologia , Animais , Bovinos , Contagem de Células , Sobrevivência Celular , Galinhas , Endotélio Corneano/citologia , Humanos , Pessoa de Meia-Idade , Técnicas de Cultura de Órgãos
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