Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 42
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Talanta ; 206: 120204, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514833

RESUMO

As a rapid and facile means for foodborne bacteria detection in situ, lateral flow immunoassay (LFA) still has intrinsic limitations in the construction of the existing sandwich LFA format, e.g. screening difficulties of paired antibodies (Abs), poor stability of Ab probe, etc. Here, combined the strong affinity of antibiotic with the superior specificity of antibody molecules, a novel and robust LFA based on a dual recognition strategy and magnetic separation was designed to achieve specific and sensitive determination of Salmonella enteritidis (S. enteritidis). In this work, ampicillin (Amp), a broad-spectrum antibiotic against bacteria, was employed as an ideal Ab replacer to anchor cells of target bacteria. By coating Amp on magnetite nanoparticles (MNPs), the Amp-MNPs showed remarkable binding, separation and enrichment capacities toward bacteria even under complex sample matrices. To ensure the selectivity of this protocol, anti-S. enteritidis monoclonal antibody was then adopted as the second anchoring agent to form a sandwich complex with Amp-MNPs. Based on these facts, S. enteritidis, as low as 102-103 CFU/mL, could be detected by naked eyes in food samples. Therefore, this creative antibiotic-bacteria-antibody LFA sandwich pattern shows great application potential in the monitoring of food contamination and infectious diseases caused by pathogenic bacteria. Compared to the common paired Abs based sandwich method, the proposed approach was cost-effective, non-labor intensive, stable, sensitive and efficient.

2.
ACS Appl Mater Interfaces ; 11(35): 31649-31660, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31407880

RESUMO

Synergistic therapeutic strategies for bacterial infection have attracted extensive attentions owing to their enhanced therapeutic effects and less adverse effects compared with monotherapy. Herein, we report a novel synergistic antibacterial platform that integrates the nanocatalytic antibacterial therapy and photothermal therapy (PTT) by hemoglobin-functionalized copper ferrite nanoparticles (Hb-CFNPs). In the presence of a low concentration of hydrogen peroxide (H2O2), the excellent Fenton and Fenton-like reaction activity of Hb-CFNPs can effectively catalyze the decomposition of H2O2 to produce hydroxyl radicals (·OH), rendering an increase in the permeability of the bacterial cell membrane and the sensitivity to heat. With the assistance of NIR irradiation, hyperthermia generated by Hb-CFNPs can induce the death of the damaged bacteria. Additionally, owing to the outstanding magnetic property of Hb-CFNPs, it can improve the photothermal efficiency by about 20 times via magnetic enrichment, which facilitates to realize excellent bactericidal efficacy at a very low experimental dose (20 µg/mL). In vitro antibacterial experiment shows that this synergistic antibacterial strategy has a broad-spectrum antibacterial property against Gram-negative Escherichia coli (E. coli, 100%) and Gram-positive Staphylococcus aureus (S. aureus, 96.4%). More importantly, in vivo S. aureus-infected abscess treatment studies indicate that Hb-CFNPs can serve as an antibacterial candidate with negligible toxicity to realize synergistic treatment of bacterial infections through catalytic and photothermal effects. Accordingly, this study proposes a novel, high-efficiency, and multifunctional therapeutic system for the treatment of bacterial infection, which will open up a new avenue for the design of synergistic antibacterial systems in the future.

3.
Chem Commun (Camb) ; 55(72): 10705-10708, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31429429

RESUMO

Electrocatalytic nitrogen reduction reactions (ENRR) can produce ammonia from nitrogen and water under ambient conditions. Here, we report the morphology-dependent electro-catalytic nitrogen reduction on Ag triangular nanoplates. Boosted by potassium cations, Ag triangular nanoplates with sharp edges exhibit a high faradaic efficiency of 25% with an ammonia yield of 58.5 mg gAg-1 h-1 at a low overpotential of -0.25 V vs. RHE. In comparison, rounded Ag nanoparticles mainly enclosed by {111} and {100} surfaces show a much smaller faradaic efficiency of 16% and ammonia yield of 38 mg gAg-1 h-1 at a larger overpotential (-0.35 V vs. RHE).

4.
Spectrochim Acta A Mol Biomol Spectrosc ; 223: 117364, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31323491

RESUMO

Mercury (II) ions (Hg2+), as one of the most toxic heavy metals, can cause irreversible damage to human health even at very low concentration due to its high toxicity and bioaccumulation. Herein, a facile ratiometric fluorescence nanomixture based on carbon dots­gold nanoclusters (CDs-Au NCs) was constructed for quantitative detection of Hg2+. Lysine functionalized carbon dots (CDs) were prepared by one-pot hydrothermal method, while gold nanoclusters (Au NCs) were synthesized via using chicken egg white (CEW) as reducer and stabilizer. The novel nanomixture exhibited two strong emission peaks at 450 nm and 665 nm under 390 nm excitation, and showed pink fluorescence under UV light. Interestingly, the fluorescence of the CDs-Au NCs nanomixture was selectively response to Hg2+. The fluorescence of Au NCs at 665 nm was decreased when Hg2+ was presented in the solution, while the fluorescence of CDs at 450 nm stayed constant. The fluorescence color changed from pink to blue obviously with increasing the concentration of Hg2+, which indicated that CDs-Au NCs could be used for visual detection Hg2+ by the naked eye. Under optimal conditions, this ratiometric fluorescent sensor could detect Hg2+ accurately and possess a great sensitivity with a detection limit of 63 nM. In addition, this method was applied to detect Hg2+ in real water samples with great recoveries, suggesting its potential in practical application with simplicity, environmentally friendly and low cost.

5.
Mikrochim Acta ; 186(7): 399, 2019 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-31183545

RESUMO

Silicon-doped carbon quantum dots (Si-CQDs) were employed to fabricate a ratiometric fluorometric probe that shows high selectivity for hydroquinone (HQ). The Si-CQDs were prepared through hydrothermal treatment of N-[3-(trimethoxysilyl)propyl]-ethylenediamine. If HQ is oxidized in a solution of the Si-CQDs, 1,4-benzoquinone will be formed which quenches the blue fluorescence (with excitation/emission peaks at 360/435 nm) of the Si-CQDs. Simultaneously, intense green fluorescence (with a emission peak at 513 nm) appears, probably due to the formation of n-π clathrates or of a quinone imine between 1,4-benzoquinone and amino groups on the surface of the Si-CQDs. The ratio of the green and blue fluorescence can be applied to the determination of HQ with a 0.077 µM detection limit. The analytical range extends from 1 to 40 µM. Graphical abstract Schematic of a silicon-doped carbon quantum dot-based ratiometric fluorescence probe with blue and green emission for the visual and fluorometric determination of hydroquinone.

6.
J Colloid Interface Sci ; 553: 768-777, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31254874

RESUMO

Developing a facile method to fabricate new heterogeneous Metal-Organic Framework (MOFs) based catalysts with high catalytic activity and stability has drawn significant attention. Herein, we demonstrate a simple in-situ pyrolysis reduction strategy to fabricate a novel three-dimensional (3D) Cu-based catalyst, which displays an outstanding performance for the decomposition of 4-nitrophenol (4-NP). Detailed characterization including SEM, FTIR, XPS, ICP-OES, HRTEM, SAED, XRD and BET confirmed the formation of the Cu/C porous composites (Cu/C-PC). Taking advantage of enormous Cu particles in the composite as well as ultrahigh surface area (196.7 m2/g) of carbon support, Cu/C-PC presents prominent catalytic activity for the hydrogenation reduction 4-NP to 4-aminophenol (4-AP) with apparent rate constant (Kapp) of 0.0267 s-1 (the ratio of Kapp to the catalyst amount is 119 s-1 g-1), which is dramatically higher than that previous reports. On the contrary, after being washed successively (Cu/C-PC-AW) by FeCl3, HCl aqueous solution and deionized water, the Cu/C porous composite materials exhibit fairly weak catalytic activity. The catalytic performance of Cu/C-PC is better than Cu, Cu2O and CuO nanoparticles as well as other catalysts in previous reports. Furthermore, Cu/C-PC shows excellent reusability, indicating its potential applications in treatment of water pollution.

7.
Food Chem ; 297: 124969, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31253282

RESUMO

The wide use of tetracyclines (TCs) for prevention and therapy of animal diseases may result in excessive residues in animal products, which could pose serious risks to human health. A novel molybdenum disulfide nanoplates (MoS2 NPs)-based fluorescent sensor for tetracycline (TET) is reported. The MoS2 NPs, synthesized via a facile bottom-up hydrothermal route, showed blue fluorescence at 430 nm in aqueous solution. Interestingly, its fluorescence was quenched significantly upon addition of TET, which is mainly due to a combination of the inner filter effect and electron transfer. Thus, the MoS2 NPs based fluorescence sensor was delineated for the detection of TET. The methodology here presented showed a low detection limit of 0.032 µM and satisfied recoveries from 88.46% to 108.62% in spiked milk, milk powder and bovine muscle samples.


Assuntos
Dissulfetos/química , Contaminação de Alimentos/análise , Leite/química , Molibdênio/química , Nanoestruturas/química , Tetraciclinas/análise , Animais , Antibacterianos/análise , Bovinos , Fluorescência , Análise de Alimentos/instrumentação , Análise de Alimentos/métodos , Limite de Detecção , Músculo Esquelético/química , Carne Vermelha/análise , Sensibilidade e Especificidade , Espectrometria de Fluorescência/métodos
8.
Nanoscale ; 11(20): 10072-10079, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-31089635

RESUMO

Photocatalytic nitrogen fixation can produce ammonia from nitrogen and water under ambient conditions in the presence of sunlight. Here, we report that alkali metal cations (Li+, Na+, and K+) can significantly promote nitrogen activation and plasmonic nanocrystals (Au and Ag) can sensitize photocatalysts under visible light. The ammonia yield and selectivity on Au/P25 under UV-vis irradiation could be increased from 0.085 mmol g-1 h-1 and 75% to 0.43 mmol g-1 h-1 and 94.5% when promoted by K+, showing a visible-light-driven activity of 0.14 mmol g-1 h-1 and an AQE of 0.62% at 550 nm. The activity could be further increased to 1.02 (UV-vis) and 0.32 (visible) mmol g-1 h-1 with AQE of 0.93% at 550 nm with methanol added as the sacrificial agent. This strategy could be applied to a series of photocatalysts (e.g. TiO2, ZnO, and BiOBr) and may represent a general approach for designing efficient nitrogen fixation processes.

9.
J Agric Food Chem ; 67(23): 6642-6649, 2019 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-31117488

RESUMO

The rapid monitoring of foodborne pathogens by monoclonal antibody (McAb)-based immunochromatographic tests (ICTs) is desirable but highly challenging as a result of the screening obstacle for a superior performance probe, which will greatly determine the capture efficiency of targets and the sensitivity of the immunoassay. In this work, on the basis of two-dimensional (2D) nanosheets (including MoS2 and graphene) as the extraordinary capture probe and signal indicator, we fabricated a label-free ICT method for Salmonella enteritidis detection. Especially, without the customarily labeled antibody probe, these 2D versatile probes presented strong capture ability toward bacteria by directly assembling onto the surface of bacteria. An ideal analytical performance with high sensitivity and specificity was achieved by virtue of the novel nanosheet-bacteria-McAb sandwich format. On the basis of MoS2 2D nanosheets as a fabulous probe element, the developed ICT exhibited a lowest detectable concentration of 103 colony-forming units/mL for S. enteritidis and could be well-applied in drinking water and watermelon juice samples. By the smart design, this work removes a series of conditionality issues of traditional double antibody sandwich-based ICTs and can give a new application direction for 2D nanosheet materials in the rapid detection field.


Assuntos
Água Potável/microbiologia , Sucos de Frutas e Vegetais/microbiologia , Imunoensaio/métodos , Salmonella enteritidis/isolamento & purificação , Citrullus/química , Citrullus/microbiologia , Grafite/química , Imunoensaio/instrumentação , Nanoestruturas/química , Salmonella enteritidis/química , Sensibilidade e Especificidade
10.
Food Chem ; 274: 816-821, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30373015

RESUMO

Here, a facile, label-free and sensitive lateral flow strip (LFS) biosensor for foodborne pathogens was established relying on the innovative introduction of Gram staining and the direct immunoreaction. Target bacteria can be directly marked with crystal violet (CV) by one-step staining which is superior to traditional signal marking techniques in LFS assay, and the method's selectivity can be guaranteed by high-specificity monoclonal antibody. With Salmonella Enteritidis (S. Enteritidis) as a model target, this protocol can selectively detect 80 CFU mL-1S. Enteritidis within 11 min in the optimized conditions. Moreover, with Listeria monocytogenes as another model target, the biosensor shows a high universality for detections of both gram-negative and gram-positive bacteria. The unexpected applicability of biological dye tracer in strip biosensor reveals that the biological dye can be a potential tool serving as a universal signal tracer for pathogenic microorganisms in food safety monitoring and early clinical diagnosis.


Assuntos
Técnicas Biossensoriais/métodos , Microbiologia de Alimentos/métodos , Listeria monocytogenes/imunologia , Salmonella enteritidis/imunologia , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Água Potável/microbiologia , Inocuidade dos Alimentos/métodos , Violeta Genciana , Limite de Detecção , Lycopersicon esculentum/microbiologia , Fenazinas , Carne Vermelha/microbiologia
11.
J Genet ; 97(5): 1379-1388, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30555086

RESUMO

Conyza blinii H. Lév., the most effective component is saponin, is a biennial medicinal material that needs to be overwintered. WRKY transcription factors family is a large protein superfamily that plays a predominant role in plant secondary metabolism, but their characteristics and functions have not been identified in C. blinii. The CbWRKY24 sequence was selectedfrom the transcriptome database of the C. blinii leaves constructed in our laboratory. Phylogenetic tree analysis revealed that it was associated with AaWRKY1 which can regulate artemisinin synthesis in Artemisia annua. Expression analysis in C. blinii revealed that CbWRKY24 was mainly induced by methyl jasmonate (MeJA) and cold treatments. Transcriptional activity assay showed that it had an independent biological activity. Overexpression of CbWRKY24 in transient transformed C. blinii resulted in improved totalsaponins content, which was attributed to upregulate the expression level of keys genes from mevalonate (MVA) pathway in transient transformed plants compared to wild type (WT) plants. Meanwhile, overexpression the CbWRKY24 in transient transformed tomato fruits showed that the transcript level of related genes in lycopene pathway decreased significantly when compared to WT tomatofruits. Additionally, the MeJA-response-element was found in the promoter regions of CbWRKY24 and the histochemical staining experiments showed that promoter had GUS activity in transiently transformed tobacco leaves. In summary, our results indicated that we may have found a transcription factor that can regulate the biosynthesis of terpenoids in C. blinii.


Assuntos
Conyza/genética , Ciclopentanos/farmacologia , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Oxilipinas/farmacologia , Proteínas de Plantas/genética , Fatores de Transcrição/genética , Temperatura Baixa , Conyza/metabolismo , Perfilação da Expressão Gênica , Filogenia , Reguladores de Crescimento de Planta/farmacologia , Folhas de Planta/genética , Proteínas de Plantas/classificação , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Elementos de Resposta/genética , Saponinas/metabolismo , Terpenos/metabolismo , Fatores de Transcrição/classificação , Fatores de Transcrição/metabolismo
12.
Parasit Vectors ; 11(1): 522, 2018 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-30236147

RESUMO

BACKGROUND: Blastocystis is one of the most common intestinal parasites in humans and animals worldwide. At least 17 subtypes have been identified in mammals and birds. In China, although some studies have reported the occurrence of Blastocystis in humans and animals, our understanding of the role of animals in the transmission of human blastocystosis is only superficial due to a paucity of available molecular data. The aim of the present study was to understand infection rates of Blastocystis and the distribution and genetic diversity of subtypes in various mammal and bird species in northeastern China, as well as to assess the zoonotic potential of Blastocystis isolates. METHODS: A total of 1265 fresh fecal specimens (1080 from ten mammal species and 185 from eight bird species) were collected in Heilongjiang, Liaoning and Jilin provinces of China. Each specimen was examined for the presence of Blastocystis by PCR amplification and sequence analysis of the partial SSU rRNA gene. RESULTS: Fifty-four specimens (4.3%) were positive for Blastocystis. Birds (7.0%) had a higher infection rate of Blastocystis than mammals (3.8%). Blastocystis was found in seven mammal species, reindeer (6.7%), sika deer (14.6%), racoon dogs (7.5%), Arctic foxes (1.9%), dogs (2.9%), rats (3.7%) and rabbits (3.3%), as well as three bird species, pigeons (2.1%), chickens (13.0%) and red crowned cranes (14.0%). Eight subtypes were identified including ST1 (n = 5), ST3 (n = 3), ST4 (n = 13), ST6 (n = 8), ST7 (n = 6), ST10 (n = 13), ST13 (n = 4) and ST14 (n = 2). 64.8% (35/54) of Blastocystis isolates belonged to potentially zoonotic subtypes. CONCLUSIONS: To our knowledge, this is the first report of Blastocystis in reindeer (ST10 and ST13), rabbits (ST4), racoon dogs (ST3) and Arctic foxes (ST1, ST4 and ST7). The findings of potentially zoonotic subtypes suggest that the animals infected with Blastocystis might pose a threat to human health. These data will improve our understanding of the host range and genetic diversity of Blastocystis, and also help develop efficient control strategies to intervene with and prevent the occurrence of human blastocystosis in the investigated areas.


Assuntos
Doenças das Aves/epidemiologia , Infecções por Blastocystis/epidemiologia , Blastocystis/genética , Variação Genética , Enteropatias Parasitárias/epidemiologia , Animais , Doenças das Aves/parasitologia , Aves , Blastocystis/isolamento & purificação , Infecções por Blastocystis/parasitologia , China/epidemiologia , Estudos Transversais , Fezes/parasitologia , Técnicas de Genotipagem , Especificidade de Hospedeiro , Humanos , Enteropatias Parasitárias/parasitologia , Mamíferos , Zoonoses
13.
Food Chem ; 261: 131-138, 2018 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-29739573

RESUMO

We presented a signal amplified lateral flow assay (LFA) based on magnetite nanoparticles (MNPs) labeled dual-probe and applied it in the high sensitive and rapid on-site detection of furazolidone metabolite of 3-amino-2-oxazolidinone (AOZ). The amplified signal benefited from high affinity between two probes of MNPs labeled murine monoclonal antibody (MNPs-MAb) and goat anti-mouse antibody (MNPs-GAMA) and was achieved by the generation of dual-probe network complex. This developed method could realize high sensitive detection of AOZ with a threshold value of 0.88 ng mL-1 and a detection limit of 0.044 ng mL-1, the sensitivity was at least 10-fold improved than that of the traditional gold nanoparticle based LFA. This facile developed assay was successfully applied for rapid detection of AOZ in milk samples. The proposed method paves a new way for on-site screening of other hazardous substances in food and can be referred in all lateral flow assays.


Assuntos
Análise de Alimentos/métodos , Furazolidona/análise , Nanopartículas de Magnetita/química , Leite/química , Animais , Furazolidona/química , Limite de Detecção , Oxazolidinonas/análise
14.
Food Chem ; 262: 48-55, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29751920

RESUMO

Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.


Assuntos
Técnicas Biossensoriais/métodos , Cromatografia de Afinidade/métodos , Clembuterol/análise , Escherichia coli , Nanopartículas Metálicas/química , Animais , Anticorpos/imunologia , Antígenos , Cromatografia de Afinidade/instrumentação , Feminino , Ouro/química , Limite de Detecção , Camundongos Endogâmicos BALB C , Sensibilidade e Especificidade
15.
Anal Bioanal Chem ; 410(13): 3161-3170, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29594429

RESUMO

Small molecules are difficult to detect by conventional gold lateral flow assay (GLFA) sensitively because the test system must satisfy the conflict requirements between enough signal intensity and limited antibody (Ab) amount. In this work, a paired labels recognition (PLR)-based biosensor was designed by utilizing the specific binding of Ab and secondary antibody (anti-Ab) to enhance signal intensity and reduce antibody amount applied in small molecule detection. The PLR amplification system is fabricated by self-assembling the common detection probe, Au-labeled Ab (Au-Ab), and the signal booster, Au-labeled anti-Ab (Au-anti-Ab). Benefiting from this, a powerful network structure can be generated to accumulate numerous gold nanoparticles (GNPs) and thus significantly strengthen the signal intensity of detection. Therefore, a lower Ab amount will be applied to offer adequate signal strength, and further, the limit of detection will be obviously downregulated due to the more effective competition reaction. Using furazolidone (FZD) as a model analyte, we achieve a detection limit of as low as 1 ng mL-1, which was at least fivefold improved over that of the traditional GLFA. Furthermore, the practicality of this strategy was certificated in five different food samples. Graphical abstract A paired labels recognition (PLR) amplification system is fabricated by self-assembling the common detection probe, Au-labeled Ab (Au-Ab), and the signal booster, Au-labeled anti-Ab (Au-anti-Ab). In this novel strategy, owing to the recognition of both Ab and anti-Ab labeled on gold nanoparticles (GNPs), a powerful network structure can be generated to accumulate numerous GNPs and thus significantly strengthen the signal intensity of detection.


Assuntos
Anti-Infecciosos Locais/análise , Técnicas Biossensoriais/instrumentação , Análise de Alimentos/instrumentação , Furazolidona/análise , Ouro/química , Nanopartículas Metálicas/química , Fitas Reagentes/análise , Animais , Anticorpos Imobilizados/química , Galinhas , Desenho de Equipamento , Limite de Detecção , Carne/análise , Leite/química , Penaeidae , Carne Vermelha/análise , Frutos do Mar/análise , Suínos
16.
Adv Sci (Weinh) ; 3(10): 1600082, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27840798

RESUMO

Biologically relevant 1,5-diazacyclooctanes derived from polyamines and acrolein, inhibit Aß40 peptide fibrillization and significantly suppress cell cytotoxicity. Formal [4+4] cycloaddition reaction of imines is thus involved in modulating oxidative stress processes associated with neural diseases.

17.
Oncol Lett ; 11(6): 3953-3960, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27313723

RESUMO

Acute promyelocytic leukemia (APL) is a common subtype of acute myeloid leukemia in China. Since the application of arsenic trioxide and all-trans retinoic acid in the treatment of APL, the prognosis has greatly improved. However, ~20% of patients with APL relapse upon completing chemotherapy. Decreasing the relapse rate and incidence of early mortality may pose the greatest challenges for the future management of APL. Recently, Ets variant 6 (ETV6) was reported to be involved in a variety of translocations associated with hematological malignancies of myeloid and lymphoid origin. To date, little is known about the clinical implication of ETV6 rearrangement in APL. In the present study, ETV6 rearrangement was examined by split-signal fluorescence in situ hybridization in 258 adults with APL, and its association with the clinical features and outcomes of the patients was analyzed. The data suggested that ETV6 rearrangement may be an independent unfavorable prognostic factor for overall survival in APL patients.

18.
Anal Sci ; 32(3): 307-11, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26960610

RESUMO

Dark field microscopy (DFM) was employed to detect amyloid ß (Aß) fibrils-induced gold nanoparticle (AuNP) aggregation at the single-particle level, with a detection limit of 40 pM fibrils. The sensitivity of this method is higher than that of the current fibril-specific detection method using probe dye, such as thioflavin T, for which sub-µM level of fibrils are necessary. This study further proved the potential application of DFM in the analytical methods based on AuNP aggregation.


Assuntos
Peptídeos beta-Amiloides/análise , Anticorpos Imobilizados/química , Anticorpos Monoclonais/química , Técnicas de Química Analítica , Ouro/química , Nanopartículas Metálicas/química , Fragmentos de Peptídeos/análise , Peptídeos beta-Amiloides/imunologia , Anticorpos Imobilizados/imunologia , Anticorpos Monoclonais/imunologia , Técnicas de Química Analítica/instrumentação , Técnicas de Química Analítica/métodos , Microscopia , Fragmentos de Peptídeos/imunologia , Sensibilidade e Especificidade
19.
Int J Cardiol ; 176(3): 661-9, 2014 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-25217220

RESUMO

BACKGROUND: Nicorandil is able to protect the cardiomyocytes from ischemic damage, but clear benefits of nicorandil in all-cause mortality and cardiovascular events were not consistently reported in patients with ischemic heart disease (IHD). MATERIALS AND RESULTS: Cochrane, PubMed, EMBASE, CBM, CNKI and Wangfang databases were searched for randomized controlled trials. Data on all-cause mortality and cardiovascular events were collected. Nicorandil groups were pooled to perform a comparison with control groups and to get the pooled odds ratios (ORs) and associated 95% confidence intervals (CIs) for all-cause mortality, relative risks (RRs), and associated 95% CIs for cardiovascular events. STATA 11.0 software was used for all-cause mortality and cardiovascular events statistics. We retrieved 17 randomized controlled studies enrolling a total of 7305 patients. The addition of nicorandil treatment significantly reduced cardiovascular events (13.83% versus 18.01%; RR, 0.77; 95% CI, 0.69 to 0.86). No differences in all-cause mortality (3.83% versus 4.70%; OR, 0.81; 95% CI, 0.64 to 1.02), and repeat revascularization rate (13.06% versus 13.54%; RR, 0.95; 95% CI, 0.70 to 1.29) were observed. There was a weak linear association between cardiovascular events and nicorandil in IHD with diabetes (P=0.099). CONCLUSIONS: The results suggest that nicorandil as an adjunct therapy to IHD is associated with reduced cardiovascular events in patients with IHD.


Assuntos
Isquemia Miocárdica/tratamento farmacológico , Isquemia Miocárdica/mortalidade , Nicorandil/uso terapêutico , Vasodilatadores/uso terapêutico , Doenças Cardiovasculares/tratamento farmacológico , Doenças Cardiovasculares/mortalidade , Causas de Morte/tendências , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto/métodos
20.
Asian Pac J Cancer Prev ; 15(24): 10905-9, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25605199

RESUMO

PURPOSE: The aim of this study was to evaluate the diagnostic value of FNA-Tg for detecting lymph node metastases in patients with a history of differentiated thyroid cancer (DTC). MATERIALS AND METHODS: A total of 58 patients with DTC diagnosis and evidence of single or multiple suspicious cervical lymph nodes were assessed. All underwent total or near-total thyroidectomy with (35 cases) or without (23 cases) radioiodine (RAI) ablation, followed by thyroid stimulating hormone (TSH) suppression therapy. A total of 68 lymph nodes were examined by ultrasound-guided fine needle aspiration (US-FNA) for both cytological examination and FNA-Tg measurement. Serum Tg and anti-thyroglobulin antibody (TgAb) levels were also measured. Diagnostic performance including sensitivity, specificity, accuracy, positive (PPV) and negative predictive value (NPV) of FNAC and FNA-Tg were calculated and compared. The Spearman's rank correlation coefficient was used to estimate the relationship between FNA-Tg and serum TgAb. RESULTS: The FNA-Tg levels were significantly higher with DTC metastatic lymph nodes (median 927.7 ng/mL, interquartile range 602.9 ng/mL) than non-metastatic lymph nodes (median 0.1 ng/mL, interquartile range 0.4 ng/mL) (p<0.01). Considering 1.0 ng/mL as a threshold value for FNA-Tg, the sensitivity, specificity, accuracy, PPV and NPV of FNA-Tg were 95.7%, 95.5%, 95.6%, 97.8% and 91.3%, respectively. The sensitivity and accuracy of the combination of FNAC and FNA-Tg were significantly higher than that of FNAC alone (p<0.05). The diagnostic performance of FNA-Tg was not significantly different between cases with or without RAI ablation, and the serum TgAb levels did not interfere with FNA-Tg measurements. CONCLUSIONS: Measurement of FNA-Tg is useful. The combination of FNAC and FNA-Tg is more sensitive and accurate for detecting lymph node metastases in patients with a history of DTC than FNAC alone. Serum TgAbs appear to be irrelevant for measurement of FNA-Tg.


Assuntos
Adenocarcinoma Folicular/secundário , Biomarcadores Tumorais/análise , Carcinoma Papilar/secundário , Linfonodos/patologia , Tireoglobulina/análise , Neoplasias da Glândula Tireoide/patologia , Adenocarcinoma Folicular/metabolismo , Adenocarcinoma Folicular/terapia , Biópsia por Agulha Fina , Carcinoma Papilar/metabolismo , Carcinoma Papilar/terapia , Ablação por Cateter , Terapia Combinada , Feminino , Seguimentos , Humanos , Imunoensaio , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Neoplasias da Glândula Tireoide/metabolismo , Neoplasias da Glândula Tireoide/terapia , Tireoidectomia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA