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1.
Zootaxa ; 3937(3): 456-70, 2015 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-25947480

RESUMO

We describe a new species (Gomphocythere besni n. sp.) from the Tavas man-made pool in Besni town (Adiyaman, Turkey). The species has several differences from its congeners in furcal structures (forked organ, seta of caudal ramus), numbers of setae on maxillula and maxillular palp, particular ornamentation with up to eight fossae in each mesh of the reticulation and in the presence of two types of lateral pore-canals (single pore opening with a sensillum and sieve plates) on the carapace. The finding of the new species extends the known geographical distribution of the living forms of the genus further to the North. The new species was found from relatively cool (16.6 ºC) and medium oxygenated waters (7.36 mg/L) in a mixture of sand and gravel substrate. Details about its ecology and taxonomic status are also compared and discussed with other species of the same genus.


Assuntos
Crustáceos/classificação , Crustáceos/ultraestrutura , Distribuição Animal , Animais , Masculino , Especificidade da Espécie , Turquia
2.
J Mater Sci Mater Med ; 18(6): 1079-88, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17268872

RESUMO

Human gingival (HG), periodontal ligament (HPL) and alveolar bone (HAB) cells (first subculture) were cultured (10(4) cells/cm2) for 35 days in alpha-Minimal Essential Medium supplemented with 10% fetal bovine serum in the presence of (i) ascorbic acid (AA, 50 microg/mL), (ii) AA + beta-glycerophosphate (betaGP, 10 mM) and (iii) AA + betaGP + dexamethasone (Dex, 10 nM). Cultures were assessed for cell attachment and spreading, cell proliferation, alkaline phosphatase (ALP) and acid phosphatase (ACP) activities and matrix mineralization. HG cell cultures presented a high proliferation rate, a low ability to synthesize ALP and ACP and the formation of a non-mineralized extracellular matrix, regardless the experimental situation. HPL cell cultures were very sensitive to the culture conditions and showed a high proliferation rate, synthesis of moderate levels of ALP and ACP and a modest matrix mineralization in the presence of AA + betaGP + Dex. HAB cell cultures presented a growth rate lower than that of HG and HPL cells, a high ALP activity and comparatively low levels of ACP, and the ready formation of a heavy mineralized matrix in the presence of betaGP. In the three periodontal cell cultures, Dex enhanced cell proliferation and expression of osteoblastic markers. Results showed that betaGP and Dex allowed the modulation of the cell proliferation/differentiation behavior within the proposed physiological and regenerative capabilities of these periodontal cells.


Assuntos
Osso e Ossos/citologia , Técnicas de Cultura de Células/métodos , Gengiva/citologia , Ligamento Periodontal/citologia , Fosfatase Ácida/metabolismo , Fosfatase Alcalina/metabolismo , Fosfatos de Cálcio/metabolismo , Proliferação de Células , Sobrevivência Celular , Células Cultivadas , Humanos , Fatores de Tempo
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