Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 26
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
ACS Nano ; 15(9): 14105-14115, 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34351143

RESUMO

Although lithium-sulfur (Li-S) batteries have long been touted as next-generation energy storage devices, the rampant dendrite growth at the anode side and sluggish redox kinetics at the cathode side drastically impede their practical application. Herein, a dual-functional fibrous skeleton implanted with single-atom Co-Nx dispersion is devised as an advanced modificator to realize concurrent regulation of both electrodes. The rational integration of single-atomic Co-Nx sites could convert the fibrous carbon skeleton from lithiophobic to lithiophilic, helping assuage the dendritic formation for the Li anode. Meanwhile, the favorable electrocatalytic activity from the Co-Nx species affording a lightweight feature effectively enables expedited bidirectional conversion kinetics of sulfur electrochemistry, thereby inhibiting the polysulfide shuttle. Moreover, the interconnected porous framework endows the entire skeleton with good mechanical robustness and fast electron/ion transportation. Benefiting from the synergistic effects between atomically dispersed Co-Nx sites and three-dimensional conductive networks, the integrated Li-S full batteries can achieve a reversible areal capacity (>7.0 mAh cm-2) at a sulfur loading of 6.9 mg cm-2. This work might be beneficial to the development of practically viable Li-S batteries harnessing single-atom mediators.

2.
Adv Mater ; 33(43): e2103050, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34463382

RESUMO

Lithium-sulfur (Li-S) batteries are promising candidates for next-generation energy storage, yet they are plagued by the notorious polysulfide shuttle effect and sluggish redox kinetics. While rationally designed redox mediators can facilitate polysulfide conversion, favorable bidirectional sulfur electrocatalysis remains a formidable challenge. Herein, selective dual-defect engineering (i.e., introducing both N-doping and Se-vacancies) of a common MoSe2 electrocatalyst is used to manipulate the bidirectional Li2 S redox. Systematic theoretical prediction and detailed electrokinetic analysis reveal the selective electrocatalytic effect of the two types of defects, thereby achieving a deeper mechanistic understanding of the bidirectional sulfur electrochemistry. The Li-S battery using this electrocatalyst exhibits excellent cyclability, with a low capacity decay rate of 0.04% per cycle over 1000 cycles at 2.0 C. More impressively, the potential for practical applications is highlighted by a high areal capacity (7.3 mAh cm-2 ) and the construction of a flexible pouch cell. Such selective electrocatalysis created by dual-defect engineering is an appealing approach toward working Li-S systems.

3.
Cancer Cell Int ; 21(1): 392, 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34289835

RESUMO

BACKGROUND: Long noncoding RNAs (lncRNAs) have emerged as crucial regulators in various cancers. However, the functional roles of most lncRNA in papillary thyroid cancer (PTC) are not detailly understood. This study aims to investigate the biological function and molecular mechanism of lncRNA Fer-1 like family member 4 (FER1L4) in PTC. METHODS: The expression of FER1L4 in PTC was determined via operating quantitative real-time PCR assays. Meanwhile, the clinical significance of FER1L4 in patients with PTC was described. The biological functions of FER1L4 on PTC cells were evaluated by gain and loss of function experiments. Moreover, animal experiments were performed to reveal the effect on tumor growth. Subcellular distribution of FER1L4 was determined by fluorescence in situ hybridization and subcellular localization assays. Luciferase reporter assay and RNA immunoprecipitation assay were applied to define the relationship between FER1L4, miR-612, and Cadherin 4 (CDH4). RESULTS: Upregulated expression of FER1L4 in PTC tissues was positively correlated with lymph node metastasis (P = 0.020), extrathyroidal extension (P = 0.013) and advanced TNM stages (P = 0.013). In addition, knockdown of FER1L4 suppressed PTC cell proliferation, migration, and invasion, whereas ectopic expression of FER1L4 inversely promoted these processes. Mechanistically, FER1L4 could competitively bind with miR-612 to prevent the degradation of its target gene CDH4. This condition was further confirmed in the rescue assays. CONCLUSIONS: This study first demonstrates FER1L4 plays an oncogenic role in PTC via a FER1L4-miR-612-CDH4 axis and may provide new therapeutic and diagnostic targets for PTC.

4.
Artigo em Inglês | MEDLINE | ID: mdl-34216749

RESUMO

We identified the prognostic factors of resected stage IA non-small cell lung cancer (NSCLC) and developed a nomogram, with purpose of defining the high-risk population who may need closer follow-up or more intensive care. Eligible stage IA NSCLC cases from the Surveillance, Epidemiology, and End Results (SEER) database and the Sun Yat-sen University Cancer Center (SYSUCC) were included. Stage IB NSCLCs were also included for evaluating the risk stratification efficacy. Cancer specific survival (CSS) was compared between groups. Statistically significant factors from multivariate analysis were entered into the nomogram. The performance of the nomogram was evaluated by concordance index (C-index) and calibration plots. A total of 23,112 NSCLC cases (SEER stage IA training cohort, N=7,777; SEER stage IA validation cohort, N=7,776; SEER stage IB cohort, N=7,559) from the SEER database were included. 1,304 NSCLC cases (SYSUCC stage IA validation cohort, N=684; SYSUCC stage IB cohort, N=620) from the SYSUCC were also included. Younger age, female, lobectomy, well differentiated, smaller size and more examined lymph nodes were identified as favorable prognostic factors. A nomogram was established. The C-index was 0.68 (95%CI, 0.67-0.69), 0.66 (95% CI, 0.64-0.68) and 0.66 (95% CI, 0.61-0.71) for the SEER training cohort, SEER validation cohort and SYSUCC validation cohort. A risk classification system was constructed to stratify stage IA NSCLC into low-risk subgroup and high-risk subgroup. The CSS curves of these two subgroups showed statistically significant distinctions. This nomogram delivered a prognostic prediction for stage IA NSCLC and may aid individual clinical practice.

5.
J Thorac Dis ; 13(4): 2363-2377, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34012585

RESUMO

Background: According to the National Comprehensive Cancer Network (NCCN) guidelines, surveillance or adjuvant chemoradiation is recommended for patients with completely resected pT2-4aN0M0 esophageal carcinoma (EC). Due to this population's variant prognosis, we developed novel nomograms to define the high-risk patients who may need closer follow-up or even post-operative therapy. Methods: Cases with resected pT2-4aN0M0 EC from the Surveillance, Epidemiology, and End Results (SEER) database and the Sun Yat-sen University Cancer Center (SYSUCC) were enrolled in the study. The SEER database cases were randomly assigned into the training cohort (SEER-T) and the internal validation cohort (SEER-V). Cases from the SYSUCC served as the external validation cohort (SYSUCC-V). Overall survival (OS) and cancer specific survival (CSS) were compared between groups. Multivariate analyses were applied to identify the prognostic factors. Nomograms and risk-classifying systems were developed. The nomograms' performances were evaluated by concordance index (C-index), calibration plots and decision curve analysis (DCA). Results: A total of 2,441 eligible EC cases (SEER-T, n=839; SEER-V, n=279; SYSUCC-V, n=1,323) were included. Age, sex, chemotherapy, lymph node harvested (LNH) and T stage were identified as the independent predictors for CSS. Regarding OS, it also included the prognostic factor of histology. Nomograms were formulated. For CSS, the C-index was 0.68 [95% confidence interval (CI): 0.66-0.71], 0.67 (95% CI: 0.63-0.71) and 0.61 (95% CI: 0.59-0.63) for the SEER-T, SEER-V, and SYSUCC-V, respectively. For OS, the C-index was 0.69 (95% CI: 0.66-0.72), 0.64 (95% CI: 0.59-0.69) and 0.62 (95% CI: 0.61-0.63) for the SEER-T, SEER-V, and SYSUCC-V, respectively. The calibration curves and DCA showed good performances of the nomograms. In further analyses, risk-classification systems stratified pT2-4aN0M0 EC into low-risk and high-risk subgroup. The OS and CSS curves of these 2 subgroups, in the full analysis set or stratified by TNM stage, histology, T stage and LNH categories, showed significant distinctions. Conclusions: The novel prognostic nomograms and risk-stratifying systems which separated resected pT2-4aN0M0 esophageal carcinoma patients into the low-risk and high-risk prognostic groups were developed. It may help clinicians estimate individual survival and develop individualized treatment strategies.

6.
Mol Oncol ; 15(5): 1597-1614, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33751775

RESUMO

With the improvement in diagnostic technology, the incidence of thyroid cancer (TC) is on the rise. Papillary thyroid carcinoma (PTC) is the most common pathological type of thyroid cancer; therefore, it is important to explore some valuable molecular targets to improve the treatment and prognosis of PTC. Studies have shown that family with sequence similarity 84, member A (FAM84A) is involved in the development of various tumors. However, the role of FAM84A in PTC remains unknown. Herein, we explored the biological function and specific molecular mechanism of FAM84A in PTC. Results indicated that FAM84A was upregulated in PTC tissues and cells. In addition, patients with higher FAM84A expression tended to possess larger tumor size, higher lymph node metastasis rate, and advanced TNM stage. Further studies indicated that downregulation of FAM84A could inhibit the development of PTC in vitro and in vivo by repressing the epithelial-mesenchymal transition (EMT) and Wnt/ß-catenin signaling pathway. Moreover, FAM84A was confirmed to be negatively regulated by tumor suppressor miR-874-3p. In conclusion, our findings suggest that FAM84A may act as a potential diagnostic and therapeutic target for PTC.

7.
Thorac Cancer ; 12(9): 1336-1346, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33751832

RESUMO

BACKGROUND: Major pathologic response (MPR) is mainly focused on residual viable tumor in the tumor bed regardless of lymph node. Herein, we investigated the predictive value of MPR and node status on survival in nonsmall-cell lung cancer (NSCLC) patients receiving neoadjuvant chemotherapy (NAC) and surgery. METHODS: A total of 194 eligible cases were included. Tumor pathologic response and node status were assessed. Based on these evaluations, patients were divided into the MPR group and the non-MPR group, the nodal downstaging (ND) group and non-ND group. Furthermore, patients were assigned into four subgroups (MPR + ND, MPR + non-ND, non-MPR + ND, and non-MPR + non-ND). Overall survival (OS) and disease-free survival (DFS) were compared between groups. Multivariate analyses were performed to identify prognostic factors. RESULTS: MPR was identified in 32 patients and ND was present in 108 patients. OS and DFS were better in the MPR group than in the non-MPR group, but with no statistical significance (OS, p = 0.158; DFS, p = 0.126). The ND group had better OS than the non-ND group (p = 0.031). However, the DFS between these two groups was comparable (p = 0.103). Further analyses suggested that both OS and DFS were better in the MPR + ND group than in the non-MPR + non-ND group (OS, p = 0.017; DFS, p = 0.029). Multivariate analyses confirmed that MPR + ND was an independent favorable predictor. CONCLUSIONS: MPR combined with ND could improve the predictive value on survival in NSCLC patients receiving NAC.

8.
ACS Nano ; 15(2): 3098-3107, 2021 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-33576601

RESUMO

The construction of aqueous Zn-ion hybrid capacitors (ZICs) reconciling high energy/power density is practically meaningful yet remains a grand challenge. Herein, a high-capacitance and long-life ZIC is demonstrated by 3D printing of a Ti3C2 MXene cathode, affording optimized carrier transport, facile electrolyte penetration, and ample porosity. The 3D-printable additive-free MXene ink with desirable rheological property is derived by a fast gelation process employing a trace amount of divalent cations, which overcomes the tedious post-treatments required for additive removal. The thus-fabricated 3D-printed (3DP) MXene cathode results in a dual-ion storage mechanism to synergize pseudocapacitive behavior of H+ and electrical double-layer capacitive behavior of Zn2+, which is systematically probed by a wide suite of in situ/ex situ electroanalytic characterizations. The 3DP MXene cathode accordingly exhibits a favorable areal capacitance of 1006.4 mF cm-2 at 0.38 mA cm-2 and excellent rate capability (184.4 F g-1 at 10 A g-1), outperforming the state-of-the-art ZICs. More impressively, ZIC full cells comprising a 3DP MXene cathode and a 3DP Zn anode deliver a competitive energy/power density of 0.10 mWh cm-2/5.90 mW cm-2 as well as an ultralong lifespan (86.5% capacity retention over 6000 cycles at 10 mA cm-2).

9.
Thorac Cancer ; 12(7): 1118-1121, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33569892

RESUMO

Parathyroid cysts (PCs) are rare, benign, cystic lesions, and PCs that occur in the mediastinum (mediastinal parathyroid cysts [MPCs]) are even more rare. Surgical resection is recommended as the first choice of treatment for MPCs. Sternotomy, thoracotomy, and thoracoscopic approaches are the most common methods for resection of MPCs. Herein, we report a case of robotic right portal minimally invasive resection of a giant nonfunctional MPC in the right anterosuperior mediastinum.


Assuntos
Cisto Mediastínico/cirurgia , Doenças das Paratireoides/cirurgia , Procedimentos Cirúrgicos Robóticos/métodos , Feminino , Humanos , Pessoa de Meia-Idade
10.
Thorac Cancer ; 12(1): 122-127, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33155374

RESUMO

Situs inversus totalis (SIT) is an extremely rare anomaly characterized by a left-to-right reversal of all the thoracic and abdominal organs. Only 11 cases of esophageal cancer with SIT have been reported worldwide, most of which underwent hybrid minimally invasive esophagectomy (MIE) but not total MIE. Here, we report a case of esophageal cancer with SIT successfully treated by total MIE, with a right lateral-prone position adopted during the thoracic procedure. The relevant literature is also discussed and reviewed.


Assuntos
Esofagectomia/métodos , Situs Inversus/cirurgia , Idoso , Humanos , Masculino
11.
Adv Mater ; 32(50): e2005967, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33179368

RESUMO

Lithium-sulfur (Li-S) batteries have heretofore attracted tremendous interest due to low cost and high energy density. In this realm, both the severe shuttling of polysulfide and the uncontrollable growth of dendritic lithium have greatly hindered their commercial viability. Recent years have witnessed the rapid development of rational approaches to simultaneously regulate polysulfide behaviors and restrain lithium dendritic growth. Nevertheless, the major obstacles for high-performance Li-S batteries still lie in little knowledge of bifunctional material candidates and inadequate explorations of advanced technologies for customizable devices. Herein, a "two-in-one" strategy is put forward to elaborate V8 C7 -VO2 heterostructure scaffolds via the 3D printing (3DP) technique as dual-effective polysulfide immobilizer and lithium dendrite inhibitor for Li-S batteries. A thus-derived 3DP-V8 C7 -VO2 /S electrode demostrates excellent rate capability (643.5 mAh g-1 at 6.0 C) and favorable cycling stability (a capacity decay of 0.061% per cycle at 4.0 C after 900 cycles). Importantly, the integrated Li-S battery harnessing both 3DP hosts realizes high areal capacity under high sulfur loadings (7.36 mAh cm-2 at a sulfur loading of 9.2 mg cm-2 ). This work offers insight into solving the concurrent challenges for both S cathode and Li anode throughout 3DP.

12.
ACS Nano ; 14(9): 11929-11938, 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-32790327

RESUMO

Electrocatalysts remain vitally important for the rational management of intermediate polysulfides (LiPSs) in the realm of Li-S batteries. In terms of transition-metal-based candidates, in situ evolution of electrocatalysts in the course of an electrochemical process has been acknowledged; nevertheless, consensus has not yet been reached on their real functional states as well as catalytic mechanisms. Herein, we report an all-chemical vapor deposition design of the defective vanadium diselenide (VSe2)-vertical graphene (VG) heterostructure on carbon cloth (CC) targeting a high-performance sulfur host. The electrochemistry induces the sulfurization of VSe2 to VS2 at Se vacancy sites, which propels the adsorption and conversion of LiPSs. Accordingly, the VSe2-VG@CC/S electrode harvests an excellent cycling stability at 5.0 C with a capacity decay of only 0.039% per cycle over 800 cycles, accompanied by a high areal capacity of 4.9 mAh cm-2 under an elevated sulfur loading of 9.6 mg cm-2. Theoretical simulation combined with operando characterizations reveals the key role played by the Se vacancy with respect to the electrocatalyst evolution and LiPS regulation. This work offers insight into the rational design of heterostructure sulfur hosts throughout defect engineering.

13.
Adv Mater ; 32(33): e2003425, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32656930

RESUMO

Zinc metal anode has garnered a great deal of scientific and technological interest. Nevertheless, major bottlenecks restricting its large-scale utilization lie in the poor electrochemical stability and unsatisfactory cycling life. Herein, a Janus separator is developed via directly growing vertical graphene (VG) carpet on one side of commercial glass fiber separator throughout chemical vapor deposition. A simple air plasma treatment further renders the successful incorporation of oxygen and nitrogen heteroatoms on bare graphene. Thus-derived 3D VG scaffold affording large surface area and porous structure can be viewed as a continuation of planar zinc anode. In turn, the Janus separator harvests homogenous electric field distribution and lowered local current density at the interface of the anode/electrolyte, as well as harnesses favorable zincophilic feature for building-up uniform Zn ionic flux. Such a separator engineering enables an impressive rate and cycle performance (93% over 5000 cycles at 5 A g-1 ) for Zn-ion hybrid capacitors and outstanding energy density (182 Wh kg-1 ) for V2 O5 //Zn batteries, respectively. This strategy with large scalability and cost-effectiveness represents a universal route to protect prevailing metal anodes (Zn, Na, K) in rechargeable batteries.

14.
Eur J Cardiothorac Surg ; 57(6): 1181-1188, 2020 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-32016340

RESUMO

OBJECTIVES: We investigated the impact of level 4 (L4) lymph node dissection (LND) on overall survival (OS) in left-side resectable non-small-cell lung cancer (NSCLC), with the aim of guiding lymphadenectomy. METHODS: A total of 1929 patients with left-side NSCLC who underwent R0 resection between 2001 and 2014 were included in the study. The patients were divided into a group with L4 LND (L4 LND+) and a group without L4 LND (L4 LND-). Propensity score matching was applied to minimize selection bias. The Kaplan-Meier method and Cox proportional hazards model were used to assess the impact of L4 LND on OS. RESULTS: A total of 317 pairs were matched. Of the cohort of patients, 20.3% (391/1929) had L4 LND. Of these patients, 11.8% (46/391) presented with L4 lymph node metastasis. L4 lymph node metastasis was not associated with the primary tumour lobes (P = 0.61). Before propensity score matching, the 5-year OS was comparable between the L4 LND+ and L4 LND- groups (69.0% vs 65.2%, P = 0.091). However, after propensity score matching, the 5-year OS of the L4 LND+ group was much improved compared to that of the L4 LND- group (72.9% vs 62.3%, P = 0.002) and L4 LND was an independent factor favouring OS (hazard ratio 0.678, 95% confidence interval 0.513-0.897; P = 0.006). Subgroup analysis suggested that L4 LND was an independent factor favouring OS in left upper lobe tumours. CONCLUSIONS: In patients with left-side operable NSCLC, L4 lymph node metastasis was not rare and L4 LND should be routinely performed.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Carcinoma Pulmonar de Células não Pequenas/cirurgia , Humanos , Neoplasias Pulmonares/cirurgia , Excisão de Linfonodo , Linfonodos/cirurgia , Estadiamento de Neoplasias , Pontuação de Propensão , Estudos Retrospectivos
15.
Environ Sci Technol ; 54(6): 3714-3724, 2020 03 17.
Artigo em Inglês | MEDLINE | ID: mdl-32069034

RESUMO

Bimetallic Fe-Mn oxide (BFMO) has been regarded as a promising activator of peroxysulfate (PS), the sustained activity and durability of BFMO for long-term activation of PS in situ, however, is unclear for groundwater remediation. A BFMO (i.e., Mn1.5FeO6.35) was prepared and explored for PS-based in situ chemical oxidation (ISCO) of trichloroethylene (TCE) in sand columns with simulated/actual groundwater (SGW/AGW). The sustained activity of BFMO, oxidant utilization efficiency, and postreaction characterization were particularly investigated. Electron spin resonance (ESR) and radical scavenging tests implied that sulfate radicals (SO4•-) and hydroxyl radicals (HO•) played major roles in degrading TCE, whereas singlet oxygen (1O2) contributed less to TCE degradation by BFMO-activated Oxone. Fast degradation and almost complete dechlorination of TCE in AGW were obtained, with reaction stoichiometry efficiencies (RSE) of ΔTCE/ΔOxone at 3-5%, much higher than those reported RSE values in H2O2-based ISCO (≤0.28%). HCO3- did not show detrimental effect on TCE degradation, and effects of natural organic matters (NOM) were negligible at high Oxone dosage. Postreaction characterizations displayed that the BFMO was remarkably stable with sustained activity for Oxone activation after 115 days of continuous-flow test, which therefore can be promising catalyst for Oxone-based ISCO for TCE-contaminated groundwater remediation.


Assuntos
Água Subterrânea , Tricloroetileno , Poluentes Químicos da Água , Peróxido de Hidrogênio , Oxirredução , Óxidos , Ácidos Sulfúricos
16.
ACS Nano ; 14(1): 867-876, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31898892

RESUMO

3D printing technology has stimulated a burgeoning interest to fabricate customized architectures in a facile and scalable manner targeting wide ranged energy storage applications. Nevertheless, 3D-printed hybrid capacitor devices synergizing favorable energy/power density have not yet been explored thus far. Herein, we demonstrate a 3D-printed sodium-ion hybrid capacitor (SIC) based on nitrogen-doped MXene (N-Ti3C2Tx) anode and activated carbon cathode. N-Ti3C2Tx affording a well-defined porous structure and uniform nitrogen doping can be obtained via a sacrificial template method. Thus-formulated ink can be directly printed to form electrode architecture without the request of a conventional current collector. The 3D-printed SICs, with a large areal mass loading up to 15.2 mg cm-2, can harvest an areal energy/power density of 1.18 mWh cm-2/40.15 mW cm-2, outperforming the state-of-the-art 3D-printed energy storage devices. Furthermore, our SIC also achieves a gravimetric energy/power density of 101.6 Wh kg-1/3269 W kg-1. This work demonstrates that the 3D printing technology is versatile enough to construct emerging energy storage systems reconciling high energy and power density.

17.
Nanomicro Lett ; 12(1): 123, 2020 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34138148

RESUMO

Titanium dioxide (TiO2) has gained burgeoning attention for potassium-ion storage because of its large theoretical capacity, wide availability, and environmental benignity. Nevertheless, the inherently poor conductivity gives rise to its sluggish reaction kinetics and inferior rate capability. Here, we report the direct graphene growth over TiO2 nanotubes by virtue of chemical vapor deposition. Such conformal graphene coatings effectively enhance the conductive environment and well accommodate the volume change of TiO2 upon potassiation/depotassiation. When paired with an activated carbon cathode, the graphene-armored TiO2 nanotubes allow the potassium-ion hybrid capacitor full cells to harvest an energy/power density of 81.2 Wh kg-1/3746.6 W kg-1. We further employ in situ transmission electron microscopy and operando X-ray diffraction to probe the potassium-ion storage behavior. This work offers a viable and versatile solution to the anode design and in situ probing of potassium storage technologies that is readily promising for practical applications.

18.
Onco Targets Ther ; 12: 7501-7512, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31571903

RESUMO

Background: Thyroid cancer is the most common endocrine malignancy, papillary thyroid carcinoma (PTC) is the main form of thyroid cancer. The long non-coding RNA (lncRNA) zinc finger antisense 1 (ZFAS1) is highly expressed in various cancer tissues and it has been shown to function as a tumor promoter in various cellular processes. However, the role of ZFAS1 in PTC is not well understood currently. Thus, this study aimed to explore the potential roles of ZFAS1 in the development and progression of PTC. Material and methods: PTC tissues (n=80) and noncancerous tissues were collected. Gain- and loss-of-function assays were performed to determine the effect of ZFAS1 on proliferation in K-1 and TPC-1 cells. The ZFAS1/mir-590-3P/HMGA2 aixs were analysed in PTC cell lines. Results: We found that the expression of ZFAS1 was increased in PTC tissues and four PTC cell lines (B-CPAP, IHH-4, TPC-1, and K-1). The gain- and loss-of-function assays showed that overexpressing ZFAS1 promoted cell proliferation and inhibited cell apoptosis in PTC cells in vitro. We demonstrated that knockdown of ZFAS1 inhibits tumor growth and upregulation of ZFAS1 promotes tumor growth in vivo. Bioinformatics analysis revealed that miR-590-3p targeted the 3'-UTR of ZFAS1. The double luciferase reporter and RNA-binding protein immunoprecipitation assay demonstrated that miR-590-3p is a target of ZFAS1. Rescue experiments confirmed that miR-590-3p could reverse the effect of ZFAS1 on PTC cells. Moreover, we identified high mobility group AT-hook 2 (HMGA2) to be a downstream target of miR-590-3p and ZFAS1 which activates HMGA2 expression by sponging to miR-590-3p. Conclusion: High ZFAS1 expression level was associated with the progression of PTC, and ZFAS1 contributed to PTC progression via miR-590-3p/HMGA2 regulatory aixs. Therefore, ZFAS1 might be a potential therapeutic target for PTC intervention.

19.
Cell Death Dis ; 10(8): 620, 2019 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-31409775

RESUMO

With the increasing incidence of papillary thyroid cancer (PTC), more attention has been paid to exploring the mechanism of PTC initiation and progression. In addition, ectopic expression of long noncoding RNAs (lncRNAs) is reported to play a pivotal role in multiple human cancers. Based on these findings, we examined lncRNA ABHD11 antisense RNA 1 (ABHD11-AS1) expression and its clinical significance, biological function and mechanism in PTC. First, we analyzed thyroid ABHD11-AS1 expression in The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Then, qRT-PCR was applied to detect the expression in paired PTC tissues and adjacent normal tissues, as well as in PTC cell lines (TPC-1 and K-1) and a normal thyroid follicular epithelium cell line (Nthy-ori3-1). In addition, we validated the relationship between ABHD11-AS1 expression and clinicopathological features by the Pearson X2 test. The oncogenic role of ABHD11-AS1 and its regulation of miR-199a-5p in PTC were examined by biological assays. Finally, bioinformatics analysis and mechanism assays were used to elucidate the underlying mechanism. We found that ABHD11-AS1 was remarkably overexpressed in PTC, and high expression was related to tumor size, lymph node metastasis, extrathyroidal extension and advanced TNM stage. Moreover, ABHD11-AS1 enhanced the abilities of cell proliferation, migration, and invasion, inhibited apoptosis in vitro, promoted tumorigenesis in vivo via sponging miR-199a-5p and then induced SLC1A5 activation. In addition, rescue assays were performed to confirm the ABHD11-AS1/miR-199a-5p/SLC1A5 axis. Taken together, the data show that ABHD11-AS1 acts as a competing endogenous RNA (ceRNA) to exert malignant properties in PTC through the miR-199a-5p/SLC1A5 axis. Therefore, our study may shed light on PTC diagnosis and therapies.


Assuntos
Sistema ASC de Transporte de Aminoácidos/genética , MicroRNAs/genética , Antígenos de Histocompatibilidade Menor/genética , RNA Longo não Codificante/genética , Câncer Papilífero da Tireoide/genética , Idoso , Proliferação de Células/genética , Progressão da Doença , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Masculino , Pessoa de Meia-Idade , Intervalo Livre de Progressão , RNA Antissenso/genética , Serina Proteases/genética , Transdução de Sinais/genética , Câncer Papilífero da Tireoide/patologia
20.
ACS Nano ; 13(7): 7517-7526, 2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31150583

RESUMO

Mass production of graphene powders affording high quality and environmental benignancy serves as a prerequisite for the practical usage of graphene in multiple energy storage applications. Herein, we exploit a salt-templated CVD approach to harness the direct synthesis of nitrogen-doped graphene (NG) nanosheets and related ink dispersions in a scalable, safe, efficient, and green fashion. Thus-fabricated NG accompanying large productivity, excellent electrical conductivity, and favorable solution processability possesses implications in printable energy storage devices. With the NG-based ink in hand, self-standing 3D architectures with programmable patterns can be directly printed over a myriad of substrates. Accordingly, both electrode preparation for flexible supercapacitors and separator modification in Li-S batteries can be enabled via printing by employing our NG-based composite inks. This work thus represents a practical route for mass production of graphene inks with cost-effectiveness and eco-friendliness for emerging energy storage technology.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...