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1.
Artigo em Inglês | MEDLINE | ID: mdl-31404053

RESUMO

STUDY DESIGN: A meta-analysis OBJECTIVE.: To investigate whether robot-assisted techniques are superior to conventional techniques in terms of the accuracy of pedicle screw placement and clinical indexes. SUMMARY OF BACKGROUND DATA: Robot-assisted techniques are increasingly applied to spine surgery to reduce the rate of screw misplacement. However, controversy about the superiority of robot-assisted techniques over conventional freehand techniques remains. METHODS: We conducted a comprehensive search of PubMed, EMBASE, and Cochrane Library for potentially eligible articles. The outcomes were evaluated in terms of risk ratio (RR) or standardized mean difference (SMD) and the associated 95% confidence intervals (CIs). Meta-analysis was performed using the RevMan 5.3 software and subgroup analyses were performed based on the robot type for the accuracy of pedicle screw placement. RESULTS: Nine randomized controlled trials with 696 patients were included in this meta-analysis. The results demonstrated that the robot-assisted technique was more accurate in pedicle screw placement than the freehand technique. Subgroup analyses showed that the TINAVI robot-assisted technique was more accurate in screw positions Grade A (RR, 1.10; 95% CI, 1.06-1.14), Grade B (RR, 0.46; 95% CI, 0.28-0.75), and Grades C + D + E (RR, 0.21; 95% CI, 0.09-0.45) than the freehand technique, whereas the Renaissance robot-assisted technique showed the same accuracy as the freehand technique in screw positions Grade A, Grade B, and Grades C + D + E. Furthermore, the robot-assisted techniques showed equivalent postoperative stay, visual analogue scale scores, and Oswestry disability index scores to those of the freehand technique and shorter intraoperative radiation exposure time, fewer radiation dose and proximal facet violations but longer surgical time than the freehand technique. CONCLUSION: The robot-assisted technique is more accurate in pedicle screw placement than the freehand technique. And TINAVI robot-assisted pedicle screw placement is a more accurate alternative to conventional techniques and the Renaissance robot-assisted procedure. LEVEL OF EVIDENCE: 1.

2.
Exp Lung Res ; 45(8): 221-235, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31378088

RESUMO

Purpose: Epithelial-mesenchymal transition (EMT) involved in asthmatic airway remodeling. Thymic stromal lymphopoietin (TSLP), an epithelial-derived cytokine, was a key component in airway immunological response in asthma. But the role of TSLP in the EMT process was unknown. We aimed to access whether TSLP could induce EMT in airway epithelia and its potential mechanism. Materials and Methods: Human bronchial epithelial (HBE) cells were incubated with TSLP or transforming growth factor beta 1 (TGF-ß1) or both. SB431542 was used to block TGF-ß1 signal while TSLP siRNA was used to performed TSLP knockdown. Changes in E-cadherin, vimentin, collagen I and fibronectin level were measured by real-time PCR, western blot and immunofluorescence staining. Expressions of TGF-ß after TSLP administration were measured by real-time PCR, western blot and ELISA. Results: TSLP induced changes of EMT relevant markers alone and promoted TGF-ß1-induced EMT in HBEs. Intracellular and extracellular expression of TGF-ß1 were upregulated by TSLP. SB431542 blocked changes of EMT relevant markers induced by TSLP. Knockdown of TSLP not only reduced TSLP and TGF-ß1 expression but also inhibited changes of EMT relevant markers induced by TGF-ß1 in HBEs. Conclusions: TSLP could induce early stage of EMT in airway epithelial cells through upregulation of TGF-ß1. This effect may act as a targeting point for suppression of asthma.

3.
Hell J Nucl Med ; 22(2): 103-110, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31273351

RESUMO

OBJECTIVE: Incorporation of lutetium-177 (177Lu) into suitable molecules that are implicated in cancer pathology represents a promising approach for the diagnosis and treatment of cancer. The goal of the present study was to develop a novel 177Lu labeled radiopharmaceutical agent for both radioimaging and targeted radionuclide therapy. ANIMALS AND METHODS: Given the synthetic versatility of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) ligand as a metal chelator and high demand of sugar molecules such as deoxyglucose (DG) in cancer cells, we carried out the radiosynthesis of a novel radiopharmaceutical agent, namely, 177Lu-DOTA-DG, and utilized it for imaging of cancer and also for the targeted radiation therapy of cancer tissues. RESULTS: In this study, we developed an efficient radiochemical synthesis of 177Lu-DOTA-DG and evaluated its pharmacological properties in vitro/in vivo. Our results showed DOTA-DG can be labeled with 177Lu with excellent radiochemical yield at 90oC in 30min. The resulting 177Lu-DOTA-DG exhibited high degree of stability without significant radiolysis up to 120h in human serum and phosphate buffer. Favorable pharmacokinetics profile was demonstrated by rapid blood clearance in 4T1 murine tumor mice and heterogeneous whole body biodistribution of 177Lu-DOTA-DG. Further, Comet assay experiments indicated that cancer cells treated with 177Lu-DOTA-DG showed significant higher degree of DNA damage compared to cells treated with 177Lu3+ or non-treated cells. CONCLUSION: This study showed that there is a great potential of using 177Lu-DOTA-DG as an imaging and therapeutic agent for cancer diagnosis and treatment. Furthermore, this study provides valuable information for developing novel 177Lu-labeled radiopharmaceuticals.

4.
Contrast Media Mol Imaging ; 2019: 4201353, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31316307

RESUMO

Objective: This work evaluated the potential of 68Ga-labelledNOTA-ICG (1,4,7-triazacyclononane-1,4,7-triacetic acid indocyanine green) for liver reserve imaging. Methods: To determine the optimal conditions for generating 68Ga-NOTA-ICG, various reaction parameters were implemented. Quality control analysis was performed using different chromatography techniques. The in vitro and in vivo stability was also measured at specific time points. The radioactivity ratio between n-octanol and water was determined to evaluate the water solubility of 68Ga-NOTA-ICG. The plasma-protein binding rate of the labelled compound was determined by the methanol method. The biodistribution and imaging findings were evaluated in normal animals at different time points after injection. A preliminary imaging evaluation was performed using an animal model of hepatic ischaemia-reperfusion injury, which was confirmed by pathology. Results: 68Ga-NOTA-ICG was prepared with very high radiochemical purity (>98%) by reacting at 90°C for 10 min at pH = 3.5∼4.0, with excellent stability in vivo and in vitro (>95% 3 h postpreparation). The in vitro plasma-protein binding rate of 68Ga-NOTA-ICG was 13.01 ± 0.7%, and it showed strong water solubility (log P=-2.01 ± 0.04). We found that in addition to excretion through the biliary tract and intestines, 68Ga-NOTA-ICG can be excreted through the urinary tract. The image quality of 68Ga-NOTA-ICG was very high; imaging agent retained in the area of liver injury could clearly be observed. Conclusion: This is the first report on a 68Ga-labelled NOTA-ICG fragment for liver reserve function studies. This complex has promise as a candidate agent for liver reserve imaging.

5.
J Sci Food Agric ; 99(13): 6097-6107, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31250448

RESUMO

BACKGROUND: Reactive oxygen species (ROS) can cause DNA damage. Rice protein (RP) inhibits ROS accumulation. However, a link between the reduction of ROS-derived DNA damage and the intake of RP is far from clear. The main objective of this study is to elucidate the effects of RPs on the reduction of DNA damage in growing and adult rats. RESULTS: An intake of RP for 2 weeks significantly reduced the hepatic accumulation of ROS and 8-hydroxydeoxyguanosine (8-OHdG) in growing and adult rats, whereas the hepatic p53 content was markedly increased by RPs. After 2 weeks' feeding, the mRNA levels and protein expressions of p53, ataxia-telangiectasia mutated (ATM), and Checkpoint kinase 2 (Chk2) were up-regulated by RPs, whereas Murine Double Minute 2 (MDM2) expressions were markedly inhibited by RPs, resulting in more p53 being translocated into the nucleus. Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) was activated by RP by reducing Kelch-like ECH-associated protein 1 (Keap1), resulting in the up-regulation of antioxidant expressions of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in RP groups. CONCLUSION: Rice protein can exert an endogenous antioxidant activity to reduce ROS-derived DNA damage by activating the Nrf2-Keap1 pathway. This study suggests that the activation of the ATM-Chk2-p53 pathway might be one of the mechanisms exerted by RP for reducing DNA damage in growing and adult rats. © 2019 Society of Chemical Industry.


Assuntos
Antioxidantes/metabolismo , Dano ao DNA , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Animais , Catalase/genética , Catalase/metabolismo , Quinase do Ponto de Checagem 2/genética , Quinase do Ponto de Checagem 2/metabolismo , Glutationa/metabolismo , Glutationa Peroxidase/genética , Glutationa Peroxidase/metabolismo , Proteína 1 Associada a ECH Semelhante a Kelch/genética , Proteína 1 Associada a ECH Semelhante a Kelch/metabolismo , Fígado/metabolismo , Masculino , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo , Proteínas Proto-Oncogênicas c-mdm2/genética , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Ratos , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Proteína Supressora de Tumor p53/genética
6.
World Neurosurg ; 122: e627-e638, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31108079

RESUMO

BACKGROUND: The effects of oblique lateral interbody fusion (OLIF) and minimally invasive transforaminal lumbar interbody fusion (MI-TLIF) have not been compared by a meta-analysis. The present study aimed to summarize the radiographic and clinical outcomes of OLIF and MI-TLIF for degenerative lumbar disease. METHODS: We performed a systematic review of related studies and report the outcomes of OLIF and MI-TLIF for degenerative lumbar disease. The radiographic outcomes measures included disc height, segmental lordotic angle, lumbar lordotic angle, and fusion. The clinical and functional outcomes included operative blood loss, operative time, length of hospital stay, complications, visual analog scale, and Oswestry disability index. Data pooling and meta-analysis with the random effects model were performed to evaluate the results. RESULTS: A total of 47 studies met the inclusion criteria. Similar changes in terms of disc height, segmental lordotic angle, lumbar lordotic angle, length of hospital stay, visual analog scale, Oswestry disability index, and radiological evidence of fusion of >90% were observed between the 2 groups. The OLIF group showed less operative blood loss and operative time. The incidence of intraoperative and postoperative complications was 9.5% and 19.9% for the OLIF group and 3.5% and 8.5% for the MI-TLIF group, respectively. CONCLUSIONS: The radiographic and functional outcomes and length of hospital stay were similar between the 2 groups. The OLIF group showed advantages in operative blood loss and operative time; however, the incidence of complications in this technique was greater than that in the MI-TLIF group.

7.
J Sci Food Agric ; 99(10): 4849-4862, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31001831

RESUMO

BACKGROUND: Methionine is an essential sulfur-containing amino acid. To elucidate the influence of l-methionine on activation of the nuclear factor erythroid 2-related factor 2-antioxidant responsive element (Nrf2-ARE) antioxidant pathway to stimulate the endogenous antioxidant activity for depressing reactive oxygen species (ROS)-derived oxidative stress, male Wistar rats were orally administered l-methionine daily for 14 days. RESULTS: With the intake of l-methionine, Nrf2 was activated by l-methionine through depressing Keap1 and Cul3, resulting in upregulation of ARE-driven antioxidant expression (glutamate cysteine ligase catalytic subunit, glutamate cysteine ligase modulatory subunit, glutathione synthase (GS), catalase (CAT), superoxide dismutase (SOD), heme oxygenase 1, NAD(P)H:quinone oxidoreductase 1, glutathione reductase (GR), glutathione S-transferase (GST), glutathione peroxidase (GPx)) with increasing l-methionine availability. Upon activation of Nrf2, glutathione synthesis was increased through upregulated expression of methionine adenosyltransferase, S-adenosylhomocysteine hydrolase, cystathionine ß-synthase, cystathionine γ-lyse, glutamate cysteine ligase (GCL) and GS, while hepatic expressions of methionine sulfoxide reductases (MsrA, MsrB2, MsrB3) and hepatic enzyme activities (CAT, SOD, GCL, GR, GST, GPx) were uniformly stimulated with increasing consumption of l-methionine. As a result, hepatic content of ROS and MDA were effectively reduced by l-methionine intake. CONCLUSION: The present study demonstrates that methionine availability plays a critical role in activation of the Nrf2-ARE pathway to induce an endogenous antioxidant response for depressing ROS-derived oxidative stress, which is primarily attributed to the stimulation of methionine sulfoxide reductase expression and glutathione synthesis. © 2019 Society of Chemical Industry.


Assuntos
Antioxidantes/metabolismo , Metionina/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo , Ratos/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Animais , Glutationa , Glutationa Peroxidase/genética , Glutationa Peroxidase/metabolismo , Proteína 1 Associada a ECH Semelhante a Kelch/genética , Proteína 1 Associada a ECH Semelhante a Kelch/metabolismo , Fígado/metabolismo , Masculino , Fator 2 Relacionado a NF-E2/genética , Ratos/genética , Ratos/crescimento & desenvolvimento , Ratos Wistar
8.
Contrast Media Mol Imaging ; 2019: 2353658, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31015824

RESUMO

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by systemic, symmetrical, and erosive synovitis. RA is one of the most common disabling diseases in the clinic. The main clinical intervention strategies are early diagnosis and early treatment. This study aims to predict the diagnostic value of 68Ga-citrate and 18F-FDG PET/CT in RA by comparing and analyzing the value of 68Ga-citrate and 18F-FDG PET/CT for diagnosing type II collagen-induced arthritis (CIA) in rats. Some CIA models were established. Normal rats were selected as the control group, and 23 days and 40 days were selected as the early and late time points of arthritis, respectively. The semiquantitative analysis of CIA rats was carried out with 68Ga-citrate PET/CT and 18F-FDG PET/CT, and the ratio of the maximum standardized uptake (SUVmax) values in the regions of interest (ROIs) of the hind foot ankle joint and thigh muscle was calculated and statistically analyzed. The distribution of CIA rats in vivo at the 68Ga-citrate 90 min time point was studied, and the ankle tissues were evaluated with hematoxylin and eosin (HE) staining. 68Ga-citrate PET/CT is obviously superior to 18F-FDG PET/CT for CIA imaging, and the statistical results show that the difference between the two examination methods is statistically significant (P < 0.001). The uptake of these two radiopharmaceuticals showed the same trend in arthritis rats with different scores. The distribution of 68Ga-citrate at 90 min is consistent with the trend shown by 68Ga-citrate PET/CT. 68Ga-citrate PET/CT can reflect the inflammatory activity of affected joints in CIA rats earlier and more sensitively than 18F-FDG PET/CT, and this imaging advantage continues until the later stage of inflammation. Therefore, 68Ga-citrate PET/CT is worthy of further promotion and application in the clinical diagnosis of RA.


Assuntos
Artrite Experimental/induzido quimicamente , Artrite Experimental/diagnóstico por imagem , Colágeno Tipo II/toxicidade , Fluordesoxiglucose F18/análise , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons/métodos , Animais , Citratos/análise , Feminino , Gálio/análise , Ratos , Ratos Sprague-Dawley
9.
J Vasc Interv Radiol ; 30(7): 973-978, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30982637

RESUMO

PURPOSE: This study investigated the outcomes of endovascular treatment for type B aortic dissection (TBAD) complicated by unilateral renal ischemia and determined the associated predictors. MATERIALS AND METHODS: From January 2010 to December 2016, 44 patients (mean : 54 years of age) with TBAD complicated by a clearly involved unilateral renal artery and a decreased mean density of the unilateral renal parenchyma were enrolled. The volumes and mean densities of each kidney were generated with postprocessing software based on computed tomography angiography. The degree of renal malperfusion (RMD) was defined as the bilateral density difference-to-the mean density ratio of the healthy kidney. The primary outcomes were renal atrophy and renal dysfunction; the secondary outcomes were aorta-related complications. RESULTS: The median follow-up time was 51 months (range: 12-102 months). During follow-up, unilateral renal atrophy and renal dysfunction were observed in 12 patients (27.3%) and 7 patients (15.9%), respectively. RMD showed a moderate predictive value for renal atrophy, with an area under the characteristic curve (AUC) of 0.78. The optimal cutoff value was 27% for RMD in terms of predicting renal atrophy (sensitivity: 91.7%; specificity: 56.2%). Moreover, aorta-related adverse events occurred in 14 patients (31.8%). Preoperative abnormal creatinine level was an independent risk factor for aorta-related complications (odds ratio [OR]: 17.5; P = 0.022) and renal dysfunction (OR: 14.2; P = 0.02). CONCLUSIONS: Preoperative serum creatinine was an effective index used to predict renal and aortic outcomes in this patient cohort. Active imaging follow-up and aggressive endovascular intervention are suggested in patients with RMD >27%.

10.
Nat Commun ; 10(1): 1584, 2019 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-30952850

RESUMO

Monolayer chalcogenide semiconductors with both luminescent and ferromagnetic properties are dreamed for simultaneous polarization and detection of the valley degree of freedom in valleytronics. However, a conventional chalcogenide monolayer lacks these coexisting properties due to their mutually exclusive origins. Herein we demonstrate that robust ferromagnetism and photoluminescence (PL) could be achieved in a (Co, Cr)-incorporated single monolayer MoS2, where the ferromagnetic interaction is activated by Co ions, and the nonradiative recombination channels of excitons is cut off by Cr ions. This strategy brings a 90-fold enhancement of saturation magnetization and 35-fold enhancement of PL intensity than the pristine MoS2 monolayer. The main reasons for the coexisting ferromagnetism and PL are the electronic interactions between the impurity bands of atop Cr adatoms and substitutional Co atoms, as well as the increased content of neutral exciton. Our findings could extend the applications of two-dimensional chalcogenides into spintronics, valleytronic and photoelectric devices.

12.
J Neurosurg Spine ; : 1-8, 2019 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-30849746

RESUMO

OBJECTIVETraditional trajectory (TT) screws are widely used in lumbar fixation. However, they may require revision surgery in some instances, especially in patients with osteoporotic spines. Cortical bone trajectory (CBT) screws may potentially be used to rescue a failed TT screw and vice versa in nonosteoporotic spines. This study aimed to investigate whether a CBT screw can salvage a compromised TT screw in osteoporotic lumbar spines and vice versa.METHODSA total of 42 vertebrae from 17 cadaveric lumbar spines were obtained. Bone mineral density was measured, and a CBT screw was randomly inserted into one side of each vertebra. A TT screw was then inserted into the contralateral side. The biomechanical properties of the screws were tested to determine their insertional torque, pullout strength, and fatigue performance. After checking the screws for the failure of each specimen, the failed screw track was salvaged with a screw of the opposite trajectory. The specimen was then subjected to the same mechanical tests, and results were recorded. A repeat pullout test on TT and CBT screws was also performed.RESULTSWhen CBT screws were used to rescue failed TT screws, the original torque increased by 50%, an average of 81% of the pullout strength of the initial TT screws was retained, and the fatigue performance was equal to that of the original screws, which were considerably stronger than the loose TT screws-that is, the TT repeat screws/TT screws were 33% of the pullout strength of the initial TT screws. When the TT screws were used to salvage the compromised CBT screws, the TT screws retained 51% of the original torque and 54% of the original pullout strength, and these screws were still stronger than the loose CBT screws-that is, the loose CBT screws retained 12% pullout strength of the initial CBT screws. Fatigue performance and the ratio of the pullout strength considerably decreased between the CBT rescue screws and the original CBT screws but slightly changed between the TT rescue screws and the original TT screws.CONCLUSIONSCBT and TT screws can be applied in a revision technique to salvage each other in osteoporotic lumbar spines. Additionally, CBT and TT screws each retain adequate insertional torque, pullout strength, and fatigue performance when used for revision in osteoporotic lumbar spines.

13.
Neurosci Lett ; 703: 104-110, 2019 06 11.
Artigo em Inglês | MEDLINE | ID: mdl-30904576

RESUMO

Postoperative cognitive dysfunction (POCD) has been increasingly recognized as a significant complication after surgery, especially in senior patients. Vagus nerve stimulation (VNS) reportedly provides beneficial effects against various brain disorders, supporting a hypothesis of its protective role in POCD. However, direct stimulation of the vagus nerve is invasive, as it requires a surgical incision in the neck. Thus, we employed a non-invasive VNS method by stimulating the dermatome in the external ear, which is innervated by the vagus nerve (auricular vagus nerve stimulation; aVNS) and sought to investigate the efficacy of this method in treating surgery-induced cognitive dysfunction in an aged rat model of POCD. We observed that the treatment of aVNS alleviated postoperative memory impairment after exploratory laparotomy surgery, as demonstrated by the shorter swimming latency and distance in Morris water maze tests. Moreover, aVNS also reduced postoperative apoptosis in the hippocampus of the aged rats. Concomitant with these beneficial effects, we found that treatment with aVNS attenuated postoperative neuroinflammation (i.e., the protein level of interleukin-1ß and tumor necrosis factor-α, along with the nuclear protein expression of NF-κB) and Alzheimer's-related pathology (tau phosphorylation at AT-8 and Ser396, as well as the levels of Aß40 and Aß42) in the hippocampus of the aged rats. In conclusion, our study is the first to reveal the neuroprotective effect of aVNS against POCD. This effect might be attributed to the inhibition of neuroinflammation and Alzheimer's-related pathology. This study suggests non-invasive aVNS may serve as a promising method for clinical treatment of POCD.

14.
Cell Biol Int ; 2019 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-30839137

RESUMO

Numerous studies have demonstrated the therapeutic effect of bone mesenchymal stem cells on spinal cord injury (SCI), especially on neural stem cells (NSCs). However, the predominant mechanisms of bone mesenchymal stem cells (BMSCs) are unclear. Recently, some researchers have found that paracrine signaling plays a key role in the therapeutic capacity of BMSCs and emphasized that the protective effect of BMSCs may be due to paracrine factors. In this study, we aimed to investigate the potential mechanisms of BMSCs to protect NSCs. NSCs were identified by immunocytochemistry. The oxidative stress environment was simulated by H2 O2 (50, 100, 200 µM) for 2 h. The apoptotic rate of the NSCs was detected via flow cytometry. Lactate dehydrogenase (LDH), malondialdehyde (MDA), and superoxide dismutase (SOD) activity were evaluated via corresponding assay kits. Western blot was used to detect the expressions of Notch1, HES1, caspase-3, cleave caspase-3, Bax, and Bcl-2. We found that H2 O2 could significantly induce the apoptosis of NSCs, increase LDH, MDA levels, and decrease SOD activity by activating the Notch1 signaling pathway. DAPT (the specific blocker of Notch1) and BMSC-conditioned medium (BMSC-CM) could significantly prevent the apoptotic effect and oxidative stress injury on NSCs that were treated with H2 O2 . We also revealed that BMSC-CM could decrease the expression of Notch1, Hes1, cleave caspase-3, Bax, and increases the expression of Bcl-2 in NSCs, which was induced by H2 O2 . These results have revealed that BMSC-CM can neutralize the effect against oxidative stress injury on the apoptosis of NSCs by inhibiting the Notch1 signaling pathway.

15.
Int J Mol Sci ; 20(4)2019 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-30781587

RESUMO

To elucidate the influence of methionine, which is an essential sulfur-containing amino acid, on the antioxidant activity of rice protein (RP), methionine was added to RP (RM). The addition of methionine to RM0.5, RM1.0, RM1.5, RM2.0, and RM2.5 was 0.5-, 1.0-, 1.5-, 2.0-, and 2.5-fold of methionine of RP, respectively. Using the in vitro digestive system, the antioxidant capacities of scavenging free radicals (superoxide; nitric oxide; 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS), chelating metal (iron), and reducing power were investigated in the hydrolysates of RP and RMs. Upon pepsin-pancreatin digestion, the weakest antioxidant capacity was produced by RP. With the addition of methionine, RMs exhibited more excellent responses to free radical scavenging activities and reducing power than RP, whereas RMs did not produce the marked enhancements in iron chelating activity as compared to RP. The present study demonstrated that RMs differently exerted the free radical scavenging activities that emerged in the protein hydrolysates, in which the strongest scavenging capacities for ABTS, superoxide, and nitric oxide were RM1.5, RM2.0, and RM2.5, respectively. Results suggested that the availability of methionine is a critical factor to augment antioxidant ability of RP in the in vitro gastrointestinal tract.


Assuntos
Antioxidantes/metabolismo , Metionina/metabolismo , Proteínas de Plantas/metabolismo , Proteólise , Antioxidantes/química , Sistema Digestório/metabolismo , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/metabolismo , Trato Gastrointestinal/metabolismo , Técnicas In Vitro , Metionina/química , Óxido Nítrico/química , Óxido Nítrico/metabolismo , Oryza/química , Proteínas de Plantas/química , Proteínas de Plantas/farmacologia , Superóxidos/química , Superóxidos/metabolismo
16.
Sci Total Environ ; 656: 937-945, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30625679

RESUMO

Accurate mercury speciation measurements are critical for developing methods for mercury removal from flue gas, but the lack of reliable adsorbents has made Hg2+ selective retention challenging. Calcium oxide (CaO) loaded on porous support is promising for HgCl2 selective adsorption because of its porosity and alkaline nature. The main hypothesis investigated in this paper is if the capacity of CaO sorbent for HgCl2 selective adsorption is attributed to its basic sites, then this will be drastically impacted by the calcium precursors. We synthesized a suite of CaO/SiO2 sorbents from different precursors, including hydrated calcium oxide (CaO-HS), calcium nitrate tetrahydrate (CaO-NS), and calcium acetate monohydrate (CaO-AcS), to investigate their performance on HgCl2 selective adsorption in a fixed-bed reactor. Compared with CaO-HS and CaO-NS, CaO-AcS was demonstrated to have the strongest affinity for HgCl2 and almost complete breakthrough for Hg0. Advanced porosity and surface basicity of CaO-AcS were confirmed by characterization analysis. CaO (001) and CaO (011) facet as well as surface defects that have different unsaturated O sites were observed using the high resolution transmission electron microscope (HRTEM). Combined theoretical and experimental methods were used to study the interaction mechanisms between HgCl2 and basic sites on CaO-AcS surfaces. Density functional theory (DFT) calculations indicated all CaO surfaces weakly interact with Hg0, while four robust bonding states of HgCl2 were predicted on different basic sites with the intensity in increasing order: Monodentate < Tridendate < Bidentate < Bridging. This was consistent with HgCl2-TPD experiments that demonstrated that the four HgCl2 adsorption configurations on CaO-AcS were attributed to different unsaturated O sites. The findings in this work highlight the application potential of CaO-AcS for gaseous Hg2+ sampling and measurement from coal-fired flue gas.

17.
Ticks Tick Borne Dis ; 10(2): 454-461, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30611724

RESUMO

This study reports the etiological identification, clinical diagnosis, and the results of the local epidemiological surveillance of the first case of severe fever with thrombocytopenia syndrome virus (SFTSV) infection in 2014 in Hunan Province, China. The infected patient was isolated and closely monitored. The virus is a member of the Bunyaviridae sandfly family and is characterized by real-time PCR, electron microscopy, immunofluorescence, and whole-genome sequencing. We also detected IgG and IgM antibodies against SFTSV among the local human population and domestic animals in a serological surveillance. Prevalence of SFTSV-specific antibodies was monitored in the local population for two years after the identification of the first SFTS case. Approximately 5% (4/77) of the people who had direct contact with the patient were seropositive, which is significantly higher than the seropositivity of the general local population [1.57% (44/2800), P < 0.05]. Furthermore, the percentage of the general population who were seropositive was higher in 2015 than in 2014 (χ2 = 7.481, P = 0.006). The epidemiological investigation found that the SFTSV is epidemic in goats, cattle, and chickens in Hunan Province. The risk of infection of domestic animals can be minimized by feeding in pens rather than allowing foraging.


Assuntos
Anticorpos Antivirais/sangue , Infecções por Bunyaviridae/diagnóstico , Monitoramento Epidemiológico , Phlebovirus/isolamento & purificação , Adolescente , Adulto , Idoso , Animais , Animais Domésticos/virologia , Infecções por Bunyaviridae/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Fazendeiros , Feminino , Febre , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Lactente , Recém-Nascido , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Phlebovirus/genética , Prevalência , Reação em Cadeia da Polimerase em Tempo Real , Estudos Soroepidemiológicos , Testes Sorológicos , Adulto Jovem
18.
Phytomedicine ; 54: 149-158, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30668364

RESUMO

BACKGROUND: Danshen (Salvia miltiorrhiza, DS) and Honghua (Carthamus tinctorius, HH) are commonly used traditional Chinese medicines for activating blood and removing stasis, and DS-HH (DH) herbal pair had potential synergistic effects on promoting blood circulation. Therefore, it is essential to make clear the active components of this herbal pair for better understanding their potential synergistic effects. PURPOSE: To comprehensively evaluate the activity of DH herbal pair on physiological coagulation system of rats, and seek their potential active components by spectrum-effect relationship analysis. METHODS: The water extracts of DH herbal pair with different proportions (DS: HH = 1:1, 2:1, 3:1, 5:1, 1:5 and 1:3) were prepared. Male Sprague-Dawley rats were randomly divided into eight groups: blank group, model group, model + 1:1 (DH) group, model + 2:1 group, model + 3:1 group, model + 5:1 group, model + 1:5 group and model + 1:3 group. The intragastric administration was performed for eight times with 12 h intervals. SC40 semi-automatic coagulation analyzer was employed to determine coagulation indices. Meanwhile, HPLC and LC-MS were applied for chemical analyses of DH extracts. Finally, the active ingredients were screened by spectrum-effect relationship analysis and the activities of major predicted compounds were validated in vitro. RESULTS: Different proportions of DH extracts could significantly prolong thrombin time (TT) and activated partial thromboplastin time (APTT), increase prothrombin time (PT) and decrease fibrinogen (FIB) content, reduced whole blood viscosity (WBV) and plasma viscosity (PV), decreased erythrocyte sedimentation rate blood (ESR) compared with model group. Furthermore, fifteen highly related components were screened out by the spectrum-effect relationship and LC-MS analysis, of which caffeic acid, salvianolic acid B, hydroxysafflor yellow A and lithospermate acid had significant blood-activing effect by prolong APTT and decrease FIB content at high (0.6 mM), medium (0.3 mM) and low (0.15 mM) (except lithospermate acid) concentrations in vitro. CONCLUSIONS: DH herbal pair showed strong blood-activating effect on blood stasis rat through regulating the parameters involved in haemorheology and plasma coagulation system. Four active compounds, caffeic acid, salvianolic acid B, hydroxysafflor yellow A and lithospermate acid predicted by spectrum-effect relationship analysis had good blood-activating effect. Therefore, spectrum-effect relationship analysis is an effective approach for seeking active components in herbal pairs.


Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Carthamus tinctorius/química , Medicamentos de Ervas Chinesas/farmacologia , Salvia miltiorrhiza/química , Animais , Benzofuranos/análise , Benzofuranos/farmacologia , Sedimentação Sanguínea/efeitos dos fármacos , Ácidos Cafeicos/análise , Ácidos Cafeicos/farmacologia , Chalcona/análogos & derivados , Chalcona/análise , Chalcona/farmacologia , Cromatografia Líquida de Alta Pressão , Avaliação Pré-Clínica de Medicamentos/métodos , Medicamentos de Ervas Chinesas/química , Fibrinogênio/metabolismo , Hemorreologia/efeitos dos fármacos , Masculino , Tempo de Protrombina , Quinonas/análise , Quinonas/farmacologia , Ratos Sprague-Dawley
19.
Environ Technol ; 40(2): 154-162, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28914183

RESUMO

Desulfurization wastewater evaporation technology is used to enhance the removal of gaseous mercury (Hg) in conventional air pollution control devices (APCDs) for coal-fired power plants. Studies have affirmed that gaseous Hg is oxidized and removed by selective catalytic reduction (SCR), an electrostatic precipitator (ESP) and wet flue gas desulfurization (WFGD) in a coal-fired thermal experiment platform with WFGD wastewater evaporation. Effects of desulfurization wastewater evaporation position, evaporation temperature and chlorine ion concentration on Hg oxidation were studied as well. The Hg0 oxidation efficiency was increased ranging from 30% to 60%, and the gaseous Hg removal efficiency was 62.16% in APCDs when wastewater evaporated before SCR. However, the Hg0 oxidation efficiency was 18.99% and the gaseous Hg removal efficiency was 40.19% in APCDs when wastewater evaporated before ESP. The results show that WFGD wastewater evaporation before SCR is beneficial to improve the efficiency of Hg oxidized and removed in APCDs. Because Hg2+ can be easily removed in ACPDs and WFGD wastewater in power plants is enriched with chlorine ions, this method realizes WFGD wastewater zero discharge and simultaneously enhances Hg removal in APCDs.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Mercúrio , Carvão Mineral , Centrais Elétricas , Águas Residuárias
20.
Clin Nucl Med ; 44(3): 262-264, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30589675

RESUMO

Familial adenomatous polyposis is a rare autosomal dominant intestinal syndrome with a high rate of malignant transformation. Here, we report a 20-year-old woman with a diagnosis of familial adenomatous polyposis by pathologic examination after colonoscopy biopsy, who underwent an F-FDG PET/CT to assess the extent of this disease. The images showed diffuse elevated FDG uptake along the entire colorectum. Additionally, focal enlarged lymph nodes with increased FDG uptake were noted. These findings promoted proctocolectomy and lymphadenectomy.


Assuntos
Polipose Adenomatosa do Colo/diagnóstico por imagem , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons , Feminino , Fluordesoxiglucose F18 , Humanos , Compostos Radiofarmacêuticos , Adulto Jovem
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