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1.
Theriogenology ; 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31791612

RESUMO

Aberrant epigenetic reprogramming is a major cause of the developmental failure of embryos after somatic cell nuclear transfer (SCNT). Histone H3 lysine 9 trimethylation (H3K9me3), a histone marker of transcriptional repression, is considered a key barrier to the development of cloned embryos. In the present study, H3K9me3 levels were much higher in SCNT embryos than IVF embryos at the 4-cell and 2-cell stages. The microinjection of the kdm4a mRNA encoding an H3K9me3 demethylase significantly increased the developmental efficiency of cloned porcine embryos. Moreover, we evaluated the effect of chaetocin, an inhibitor of histone methyltransferases suv39h1/2, on SCNT embryo development. Chaetocin did not suppress the H3K9me3 modification in porcine embryonic fibroblast (PEF) but downregulated the expression of suv39h1, suv39h2, and kdm4d. However, 10 nM chaetocin treatment efficiently decreased the H3K9me3 level in cloned embryos. Importantly, a chaetocin treatment at the 4-cell stage for 6 h significantly increased the blastocyst rate and total cell numbers. Furthermore, the inhibitor treatment upregulated the expression of related developmental genes. In summary, both overexpression of kdm4a and treatment with a suv39h1/2 inhibitor improve the epigenetic reprogramming of cloned embryos and further improve the developmental competence in vitro.

2.
Geriatr Gerontol Int ; 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31803998

RESUMO

AIM: It is forecasted that the figure of families in China who lost their only child will reach 10 million by 2035. Traditional Chinese culture places these families in a particularly disadvantaged position for elderly care. It is quite important to understand the elderly care needs of the parents. METHODS: The cluster sampling method was used to select the parents who lost their only child in Wuhu city, China. Univariate and multivariate logistic regression analysis models were carried out to analyze factors influencing the parents' preferences for elderly care mode and choice of caregivers. RESULTS: The Geriatric Depression Scale score (P < 0.001) was significantly associated with the parents' preferences for elderly care mode. Parents with different sex, marital status and grandchildren status had different preferences for their choice of caregivers. The number of chronic diseases and Geriatric Depression Scale scores were significantly associated with preferences for choice of caregivers. CONCLUSIONS: These parents should have a preferential policy of check in to a nursing home and simplify the check-in procedure. The parents should receive prompt for depression and stay in the community for elderly care. The government should guarantee that spouseless parents have priority in enjoying nurse/nursing home worker services. The government should support the development of volunteer services. Geriatr Gerontol Int 2019; ••: ••-••.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31745672

RESUMO

Emerging evidence suggests that long non-coding RNAs (lncRNAs) are critical regulators of diverse biological processes, including adipogenesis. Despite being considered an ideal animal model for studying adipogenesis, little is known about the roles of lncRNAs in the regulation of rabbit preadipocyte differentiation. In the present study, visceral preadipocytes isolated from newborn rabbits were cultured in vitro and induced for differentiation, and global lncRNA expression profiles of adipocytes collected at days 0, 3, and 9 of differentiation were analyzed by RNA-seq. A total of 2066 lncRNAs were identified from nine RNA-seq libraries. Compared to protein-coding transcripts, lncRNA transcripts exhibited characteristics of a longer length and lower expression level. Furthermore, 486 and 357 differentially expressed (DE) lncRNAs were identified when comparing day 3 vs. day 0 and day 9 vs. day 3, respectively. Target genes of DE lncRNAs were predicted by the cis-regulating approach. Prediction of functions revealed that DE lncRNAs when comparing day 3 vs. day 0 were involved in gene ontology (GO) terms of developmental growth, growth, developmental cell growth, and stem cell proliferation, and involved in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of PI3K-Akt signaling pathway, fatty acid biosynthesis, and the insulin signaling pathway. The DE lncRNAs when comparing day 9 vs. day 3 were involved in GO terms that associated with epigenetic modification and were involved in the KEGG pathway of cAMP signaling pathway. This study provides further insight into the regulatory function of lncRNAs in rabbit visceral adipose and facilitates a better understanding of different stages of preadipocyte differentiation.

4.
Macromol Rapid Commun ; 40(23): e1900492, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31693258

RESUMO

Fibers have traditionally been made through melt or solution processes from macromolecules. Most of these fibers have crystalline domains where the segregation of different crystalline features is extremely difficult due to the statistical nature of the formation and growth of these domains. A fibrous nano-crystalline sandwich is reported where distinctly different crystalline regions are formed in layers along the continuous fiber direction during the spinning process and locked in place. This approach employs side-by-side bicomponent nanofiber electrospinning where the components are the enantiomeric pair of poly(l-lactic acid) and poly(d-lactic acid). The formation of the poly(lactic acid) (PLA) stereo-complexes at the junction interphase of the two components is demonstrated through diffusion, which subsequently crystallize into continuous sandwich domains. The stereo-complex crystalline core in the fiber possesses a melting point 50 °C higher than, and properties substantially different from, the regular PLAs at the fringe areas of the fiber. This nano-crystalline sandwich fiber structure can be scaled to the micrometers in a commercial bicomponent process.

5.
Biosci Rep ; 39(11)2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31642468

RESUMO

Neuron-specific enolase (NSE), also known as gamma (γ) enolase or enolase-2 (Eno2), is a form of glycolytic enolase isozyme and is considered a multifunctional protein. NSE is mainly expressed in the cytoplasm of neurons and neuroendocrine cells, especially in those of the amine precursor uptake and decarboxylation (APUD) lineage such as pituitary, thyroid, pancreas, intestine and lung. In addition to its well-established glycolysis function in the cytoplasm, changes in cell localization and differential expression of NSE are also associated with several pathologies such as infection, inflammation, autoimmune diseases and cancer. This article mainly discusses the role and diagnostic potential of NSE in some lung diseases.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31514312

RESUMO

In real life, garbage has caused great pollution to the environment. A garbage classification system is an effective way to manage this issue, and is an innovation in Shanghai, China. Innovation diffusion is the topic of this paper. This study uses a mathematical statistics method to formulate individual bounded rationality, and uses the specific graph structure of a scale-free network to characterize group structure. Then, a model of group behavior is constructed and the simulation experiment is run on the Python platform. The results show that: (1) In the case of general cognitive ability and high value innovation, most individuals in the group will accept the innovation in the process of innovation dissemination in a garbage classification system after several rounds of the game; (2) it is more helpful to improve the cognitive ability of individuals and the true value of innovation for the diffusion of innovation; and (3) the larger a group, the greater the scope of innovation diffusion and the more time is needed. It is helpful to expand the scope and reduce the time of innovation diffusion by increasing connections among individuals. The innovation of this study is the characterization of individual bounded rationality, which has a certain theoretical value. Meanwhile, the research results of this paper have important practical significance for the promotion of garbage classification, which can be used to popularize the concept of garbage classification.

7.
Crit Rev Oncol Hematol ; 143: 27-45, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31449984

RESUMO

Although paclitaxel plus ramucirumab has been recommended as the preferred second-line strategy, other regimens also display potentially comparable efficacies. Record retrieval was conducted in PubMed, Web of Science, Cochrane Central Register of Controlled Trials, Embase, ASCO and ESMO meeting libraries. Randomized controlled trials featuring comparisons between different systemic treatments among previously treated patients with advanced gastric cancer were eligible for our systematic review. Network calculation were based on random-effects model and the relative ranking of each regimen was numerically indicated by P-score (CRD42018104672). Concerning second-line regimens, "paclitaxel plus olaparib" and "paclitaxel plus ramucirumab" dominated the overall survival ranking while "paclitaxel plus ramucirumab" additionally topped the hierarchy for progression-free survival. Among refractory or third-line only cases, apatinib reigned the hierarchy by significantly and insignificantly surpassing placebo and nivolumab respectively. In conclusion, paclitaxel plus ramucirumab is the optimal second-line regimen. Both apatinib and nivolumab could be potentially recommended as refractory regimens.

8.
Zhen Ci Yan Jiu ; 44(6): 434-8, 2019 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-31368267

RESUMO

OBJECTIVE: To investigate the efficacy of acupoint injection of Ropivacaine for labor analgesia and its effect on breastfeeding and prolactin secretion. METHODS: A total of 80 primipara who asked to receive labor analgesia were randomized into epidural analgesia group (n=35) and acupoint injection group (n=36), and other 36 primipara who refused to receive labor analgesia were subjected to the control group. The labor analgesia began to be performed when the puerpera's uterine orifice opened about ≥3 cm (the first stage of labor), including puerpera controlled epidural analgesia (PCEA, Sufentainil + 0.1% Ropivacaine hydrochloride, 5 µg/mL at L3-L4 interspace, till the birth of fetus) or acupoint injection of Ropivacaine (0.2%, 1 mL/acupoint) at bilateral Hegu (LI4) and Sanyinjiao (SP6). The delivery situations such as the duration of labor, and number of cases who used oxytocin, obstetric-forceps-aided delivery, cesarean delivery were recorded. The serum prolactin concentration was assayed by using ELISA. The visual analogue scale (VAS) scores at the time points of T0 (about 3 cm widening of the orifice of uterus and before performing analgesia), T1 (30 min after labor analgesia), T2(about 10 cm widening of the orifice of uterus) and T3(coming out of fetal head). The duration of labor, ratio of use of oxytocin, onset time of breastfeeding, and times of breastfeeding within 24 postpartum hours were recorded accordingly. RESULTS: The VAS scores at time-points of T1, T2 and T3 were significantly lower in both epidural analgesia and acupoint injection groups in comparison with their own T0 and the control group (P<0.05), and were also considerably higher in the acupoint injection group than in the epidural analgesia group (P<0.05). The duration of the 2nd stage of labor (from complete opening of the uterus orifice to complete birth of the fetus) was significantly longer, (P<0.05) and the number of oxytocin-using puerpera was obviously bigger in the epidural analgesia group than in the control group (P<0.05). After partum, the 1st breastfeeding time was obviously earlier and the frequency of breastfeeding notably increased in both epidural analgesia and acupoint injection groups than in the control group (P<0.05), the serum prolactin content was remarkably higher in the acupoint injection group than in the epidural analgesia group (P<0.05). No significant differences were found between the acupoint injection and the control groups in the duration of the 1st and 2nd stages of labor, and in the numbers of oxytocin-using puerpera, obstetric forceps-aided birth and cesarean delivery (P>0.05), and between the epidural analgesia group and control group in the serum prolactin levels (P>0.05).. CONCLUSION: Injection of Ropivacaine at LI4 and SP6 is effective for labor analgesia and raising prolactin level, and favorable to breastfeeding in the early postpartum period.


Assuntos
Pontos de Acupuntura , Analgesia Obstétrica , Analgesia por Acupuntura , Anestésicos Locais , Aleitamento Materno , Feminino , Humanos , Gravidez , Prolactina , Ropivacaina
9.
Artigo em Inglês | MEDLINE | ID: mdl-31396862

RESUMO

Banana (Musa acuminata) by-products and other agricultural residues such as rice straw and sugarcane top are abundant in the southern part of China, but most of them are wasted and discarded. Under this experiment, several silages containing banana by-products with other crop residues were investigated for their fermentation characteristics and feeding values to beef cattle. There were three treatments (T) as follows: T1 = fresh banana by-products (FBBP) + 4% corn meal (CM), T2 = FBBP + rice straw (RS) at 70:30 (fresh weight), T3 = FBBP + sugarcane top (SCT) at 70:30 (fresh weight). Twenty-one beef cattle bulls (220 ± 15 kg body weight) were randomly assigned to 3 groups and assigned to one of three silage treatments in a total feeding period of 237 days. Results revealed that all of the three silage mixtures had a good fermentative profile with lower pH and higher organic acids. The FBBP + CM group significantly increased DM, CP, EE, and Ca content (P < 0.05), while decreased ash, NDF, ADF, ADL, and condensed tannins (CT) content (P < 0.01). Significant effects on weight gain, feed intake, and feed efficiency were observed at the end of the experiment (P < 0.05). The FBBP + CM group also had the greatest total weight gain at 109 kg/head, twice of the FBBP + RS group (P = 0.001), as well as the greatest average daily weight gain at 0.48 kg/head/day, followed by the FBBP + SCT group at 0.30 kg/head/day and the FBBP + RS group at 0.27 kg/head/day, respectively. Cattle fed FBBP + CM silage mixture diet had better feed efficiency than the cattle fed with the other mixtures (P < 0.001). Results of body measurements showed that cattle consumed FBBP + CM silage mixture tended to have a greater body diagonal length, height at the hip, hip width, and rump length than those either consumed FBBP + RS or FBBP + SCT (P < 0.05). The findings showed promising results of banana by-products silage with other agricultural crop residues to be used for increasing beef cattle production in southern China.

10.
Plant Physiol Biochem ; 142: 510-518, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31445476

RESUMO

As one of the most popular woody species that blooms in summer, Lagerstroemia speciosa has been used abundantly in urban landscape for its excellent floral beauty. For the first time, we discovered a double-flower variant with all petaloid stamens. To understand the molecular basis of this variation, we contrasted the transcriptomes of single- and double-flower buds at three stamen development stages. In total, 73,536 unigenes were mapped and 30,714 differently expressed genes (DEGs) were identified in the tissues. We focused on the DEGs expressing in both phenotypes and investigated the association of their expression profiles with their functions in transcription pathways. Furthermore, we performed WGCNA and identified co-expressed genes with four floral homeotic genes as hubs (MADS16, Unigene0026169; AP2, Unigene0042732; SOC1, Unigene0046314; AG, Unigene0056437). The expression of these hub genes has been conserved across the three developmental stages but significantly different between the two floral phenotypes. As a result, the robust transcriptional regulation of stamen petaloidy in double flowers was deduced. These findings will help to unravel the regulatory mechanisms of several specific genes, thereby providing a basis to study double-flower molecular breeding in L. speciosa.


Assuntos
Flores/crescimento & desenvolvimento , Lagerstroemia/crescimento & desenvolvimento , Flores/anatomia & histologia , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Genes de Plantas/fisiologia , Lagerstroemia/anatomia & histologia , Reguladores de Crescimento de Planta/fisiologia , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Transcrição/genética , Fatores de Transcrição/fisiologia
11.
Surg Endosc ; 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31451922

RESUMO

BACKGROUND: Peroral endoscopic myotomy (POEM) is a safe and effective approach for achalasia. However, the safety, feasibility, perioperative and long-term efficacy in treating geriatric patients has not been well evaluated. METHODS: Data of 2367 patients diagnosed with achalasia and treated with POEM in the Endoscopy Center, Zhongshan Hospital, Fudan University from August 2010 to December 2017 were retrospectively reviewed. Last follow-up was in December 2018. Propensity score matching based on baseline characteristics was used to adjust for confounding. With a caliper of 0.01 in propensity scoring, 139 patients aged ≥ 65 years were matched at a 1:2 ratio with 275 patients aged < 65 years. Perioperative complications and long-term outcomes were compared between the two groups. RESULTS: After propensity score matching, the two groups had similar baseline clinical characteristics and distribution of propensity scores. The mean age was 70.22 years in geriatric patients and 42.02 in younger patients. Technical failure occurred in one geriatric and one non-geriatric patients (p = 0.485). The procedural time in geriatric patients was similar to younger patients [50 (interquartile range (IQR) 36-76) vs. 50 (IQR 36-70) min, p = 0.398]. There were also no significant differences in major perioperative adverse events (2.88% vs. 2.18%, p = 0.663) and hospitalization length (median 3 vs. 3 days, p = 0.488). During a median follow-up period of 41 months (IQR 26-60), mean decrease in Eckardt score and pressure of the LES were 6.63 and 11.9 mmHg in geriatric patients, which were similar to the change in non-geriatric patients (6.49 and 11.6 mmHg, p = 0.652 and 0.872, respectively). Clinical reflux occurred in 23.53% geriatric patients and 21.59% non-geriatric patients (p = 0.724). 5-year success rate of 92.94% was achieved in geriatric patients and 92.61% in younger patients (log-rank p = 0.737). CONCLUSIONS: POEM is a safe and reliable treatment in geriatric achalasia patients with confirmed short-term and long-term efficacy compared with those in non-geriatric patients.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31336914

RESUMO

In order to realize the simulation and evaluation of road traffic noise among urban buildings, a spatial subdivision-based beam-tracing method is proposed in this study. First, the road traffic source is divided into sets of point sources and described with the help of vehicle emission model. Next, for each pair of source and receiver, spatial subdivision-based beam-tracing method is used in noise paths generation. At last, noise distribution can be got by noise calculation of all receivers considering the complex transmission among urban buildings. A measurement experiment with a point source is carried out to validate the accuracy of the method; the 0.8 m height and 2.5-m height average errors are about 0.9 dB and 1.2 dB, respectively. Moreover, traffic noise analysis under different building layouts and heights are presented by case applications and conclusions can be reached: (1) Different patterns result in different noise distributions and patterns designed as self-protective can lead to an obvious noise abatement for rear buildings. Noise differences between the front and rear buildings are about 7-12 dB with different patterns. (2) Noise value might not show a linear variation along with the height as shielding of different layers is various in reality.

13.
United European Gastroenterol J ; 7(6): 782-789, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31316782

RESUMO

Background and aims: Making an optimal and lasting submucosal cushion is critical for endoscopic submucosal dissection. The thermo-sensitive binary hydrogels system composed of poloxamer 407 and poloxamer 188 might be an excellent submucosal injection solution considering the unique feature that it remains liquid at room temperature and becomes gelatinous after being injected in the submucosa of the digestive tract. The present study focuses on preparing the thermo-sensitive binary hydrogels system and testing its capacity in mucosal lifting and its role in the endoscopic submucosal dissection procedure. Methods: Various concentrations of poloxamer 407 and poloxamer 188 were added to normal saline. The gelation temperature viscosity of the thermo-sensitive binary hydrogels system was measured to choose the best formula. The thermo-sensitive binary hydrogels system and normal saline were first compared in extracted porcine stomach. For in vivo study, the thermo-sensitive binary hydrogels system and normal saline were compared for facilitating the endoscopic submucosal dissection procedure. Results: Among the 46 kinds of thermo-sensitive binary hydrogels system, gelation temperatures of the thermo-sensitive binary hydrogels system I (poloxamer 407/poloxamer 188, 17%/0.5%, w/w) and the thermo-sensitive binary hydrogels system II (poloxamer 407/poloxamer 188, 18%/2%, w/w) were among the ideal range of gelation temperature. The injecting pressure in vitro study of thermo-sensitive binary hydrogels system II was significantly higher than that of thermo-sensitive binary hydrogels system I and normal saline (p < 0.001). Sixteen gastric endoscopic submucosal dissection procedures were performed in a porcine model. The initial volume of normal saline injection (13.88 ± 3.91 ml vs 5.88 ± 3.44 ml, p = 0.001) was significantly larger than the thermo-sensitive binary hydrogels system group. The postoperative wound showed a significant difference in the two groups (p = 0.023) indicating that the thermo-sensitive binary hydrogels system can create a cleaner wound. Conclusions: Considering the gelation temperature, viscosity, injection pressure, and the height of the mucosal elevation, the thermo-sensitive binary hydrogels system I was the better submucosal injection solution.

14.
Dalton Trans ; 48(34): 12839-12849, 2019 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-31334728

RESUMO

The new aroylhydrazone N'-(di(pyridin-2-yl)methylene)pyrazine-2-carbohydrazide (HL) species, rich in N-donor sites, has been used to synthesize Cu(ii) compounds with different nuclearities, viz. the binuclear [Cu2(µ-1κN3,2κN2O-L)(Cl)3(MeOH)] (1), the octanuclear [Cu4(µ-1κN3,2κN2O-L)2(µ-Cl)3(Cl)3]2 (2) and the 1D coordination polymer [Cu3(µ3-1κN3,2κN2O,3κN-L)(µ-NO3)(NO3)3(H2O)3]n·nNO3 (3). They have been characterized by elemental analysis, FT-IR and single crystal X-ray diffraction. The magnetic properties of 2 and 3 have been explored using variable temperature magnetic measurements. The catalytic performances of the compounds were evaluated towards the peroxidative oxidation of o-, p- and m-xylenes under microwave irradiation, leading to the formation of the corresponding methyl benzyl alcohol, tolualdehyde and toluic acid as the major products. Complex 3 exhibits the best catalytic activity towards the oxidation of p-xylene with a total yield of 37% (4-methylbenzyl alcohol + p-tolualdehyde + p-toluic acid).

15.
Anal Chim Acta ; 1077: 216-224, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31307712

RESUMO

We designed a smartphone based field-portable cell counter combining the smartphone microscope for bright-field image recording and the smartphone application for automatically cell recognition, counting and analysis. To our best knowledge, it is the first time that a smartphone based cell counter can distinguish and count both live and dead cells simultaneously. Compared to the results obtained by hemocytometer, commercial cell counter and flow cytometer, the proposed device was proved to detect cell concentration and viability accurately within the application range between 105 cells/mL and 107 cells/mL. Though multiple fields of view were measured to increase the sampling amount for error reduction, the whole operations including image recording and processing can still be finished rapidly. Moreover, the proposed device is cost-effective with small size of 170 mm × 113 mm × 168 mm containing a built-in power supply. Considering its advantages as high accuracy, fast speed, low cost, long battery life and compact configuration, it is believed the proposed device is a potential tool applied in on-site cell analysis.


Assuntos
Contagem de Células/métodos , Smartphone , Animais , Contagem de Células/instrumentação , Cercopithecus aethiops , Desenho de Equipamento , Software , Células Vero
16.
Artigo em Inglês | MEDLINE | ID: mdl-31273580

RESUMO

Tropical and subtropical regions were quite short of high-quality protein forage. Ramie (Boehmeria nivea L. Gaud) leaves as crop by products, are rich in protein and widely cultivated in tropical and subtropical regions. Thence, the development and utilization of ramie is of great significance to animal production in these regions. But it contained high level of tannins and total phenols, which may produce potentially adverse effect. It is very essential to evaluate the safety of ramie leaves before they are used as feed supplements. To evaluate potential toxic level of ramie leaf, control group, low dose and high dose (0, 1, 2 g/kg·BW) groups of ramie leaf were conducted orally in Sprague-Dawley rats (SD rats). Body weight, hematology, and histopathology was assessed during 28 d of treatment and 14 d of recovery period. The results showed that there were no toxic symptoms appeared in the treated and control groups. There were a few individual indicators showed abnormal, but most indices of body weight, organ weight ratios and hematology were normal. And compared to control group, it showed no significant differences (P>0.05). Histopathological examination of the high dose group and control group showed that there was no lesions related to ramie administration. The pathological changes appeared in the liver, and lungs of rats in individual rat of both groups were common and spontaneous, and had no significant differences (P>0.05). These results suggest that under this experimental condition, up to 2 g/kg·BW intragastric administration of ramie leaf did not produce adverse effect to SD rats. These findings would provide available information for ramie leaf to utilize as a feed supplement, particularly in P.R. China.

17.
BMC Nephrol ; 20(1): 209, 2019 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-31174507

RESUMO

BACKGROUND: Tacrolimus has been widely used for immunosuppressive therapy in solid organ transplantation (SOT) and allo-geneic stem cell transplantation (allo-SCT) over the past 2 decades. Pancreatitis caused by tacrolimus was rarely reported in kidney transplantation previously. CASE PRESENTATION: Here we presented a case of a 45-year-old male who underwent kidney transplantation and received immunosuppressive therapy of tacrolimus, on day + 67 after transplantation he developed acute pancreatitis with extremely high blood concentration of tacrolimus. We excluded other possible causes and speculated tacrolimus was the probable inducer of pancreatitis. After tacrolimus was discontinued and alternated with cyclosporine, he gradually recovered and was discharged home with no relapse. CONCLUSION: Tacrolimus can be a probable cause of pancreatitis after kidney transplantation. We recommended clinicians to be aware of the possibility of tacrolimus-induced pancreatitis during tacrolimus treatment.

18.
Eur J Surg Oncol ; 45(10): 1796-1805, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31204169

RESUMO

Different countries prefer particular types of multimodal treatments against resectable gastric cancer. Due to lacking of unified conclusions, we therefore conducted a network meta-analysis to rank all recommended strategies simultaneously and hierarchically. Record retrieval was conducted in PubMed, Web of Science, Cochrane Central Register of Controlled Trials, Embase, ASCO and ESMO meeting libraries from inception to September 2018. Randomized controlled trials featuring comparisons between different preferred multimodal treatments against resectable gastric cancer were eligible. The Cochrane Risk of Bias Tool was applied to assess methodological quality of included trials. Overall survival was primary endpoint. Network calculation was based on random-effects model and the relative ranking of each node was numerically indicated by P-score. All procedures were conducted according to Cochrane Handbook 5.1 and PRISMA for Network Meta-analysis (CRD42018109147). As a result, a total of 11 studies were included into our systematic review, corresponding to 7235 patients. Regarding overall survival, "PeriCT (FLOT)" (perioperative 5-FU plus leucovorin plus oxaliplatin plus docetaxel chemotherapy) topped the hierarchy (HR 1.00, P-score = 0.918), followed by "PostCT (XP)" (postoperative capecitabine plus platinum chemotherapy; HR 1.14, P-score = 0.759) and "PostCT (S-1)" (postoperative S-1 monotherapy; HR 1.16, P-score = 0.732). In subgroup analyses, "PostCT (XP)" became the top regimen for eastern population while "PeriCT (FLOT)" was the optimal node for western population. In conclusion, perioperative FLOT chemotherapy could potentially be the best multimodal treatment against resectable gastric cancer than other recommended strategies. Therefore, a global D2-lymphadenectomy randomized controlled trial comparing perioperative FLOT chemotherapy with postoperative XELOX chemotherapy should be carried out.

19.
J Craniomaxillofac Surg ; 47(7): 1096-1103, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31088762

RESUMO

PURPOSE: There is little knowledge on the growth of cranial defects, appropriate timing and outcomes of application of titanium mesh for cranioplasty in the pediatric population, especially pre-school age (2-5 years old) and school age (6-12 years old) children. We hypothesised that cranioplasty for pre-schoolers could be delayed to school age due to the expected cranium growth, whereas, for the school age group, it is better to perform routine cranioplasty (3-6 months) to protect the brain and therefore ensure their timely return to school life. MATERIALS AND METHODS: A retrospective review of pediatric patients (2-12 years old) who underwent titanium mesh cranioplasty for cranial defects from 2006 to 2012 was performed. Patient demographic data, radiological data, and clinical information were collected. Specifically, cranial defect sizes were evaluated by three-dimensional (3D) reconstruction of computed tomography data after craniectomy, before cranioplasty and 2-years after cranioplasty. Patients were routinely followed up at an outpatient clinic for complications and school attendance. RESULTS: A total of 18 titanium mesh cranioplasties were performed in 18 patients. The average interval between craniectomy and cranioplasty was 3 years for pre-schoolers and 4 months for the school age group. Patients in the pre-schooler group showed significant enlargements in cranial defects during the interval as compared with the school age group (26% vs. 4%, P < 0.05). There were no surgery-related complications except in one patient, who had titanium mesh exposure 11 months later. Two years after cranioplasty, there was no significant difference in mild cranial defect enlargements between the two groups (11% vs. 6%, P > 0.05). Patients were followed for an average of 5 (range, 2-8) years. All patients had satisfactory recovery of cranial contour, sufficient protection of the brain and active participation in school study. All patients had satisfactory recovery of cranial contour, sufficient protection of the brain and active participation in school. CONCLUSION: Timing of titanium mesh cranioplasty after decompressive craniectomy based on their age is a workable solution for school-age pediatric patients. The enlargement of cranium defects in pre-schoolers supports a delayed repair until school age. The long-term outcomes for these patients with titanium mesh cranioplasty are favourable.

20.
Sensors (Basel) ; 19(9)2019 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-31060264

RESUMO

Wireless sensor networks have been attracting research attention for the past decade and will continue to be a hot topic due to the emerging trend of Internet-of-Things (IoT). Edge computing for IoT (Edge-IoT) is a promising framework that can help low-powered sensor networks to conduct complex computational tasks. Different from the existing works that focus on cooperative task execution for edge and sensor networks, in this paper, we investigate the problem of reliable data transmission in edge-aided sensor networks. Firstly, we discuss how edge servers can help to improve the data transmission of sensor networks. Secondly, we propose a forwarding scheme for edge nodes to forward packets according to coverage and corresponding interference. Thirdly, we propose an edge-based error recovery approach. By employing edge servers for data transmission and error recovery, the efficiency and reliability of data transmissions can be largely improved.

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