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1.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(6): 1907-1910, 2021 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-34893132

RESUMO

OBJECTIVE: To proceed the clinical evaluation of DNA microarray for thalassemia gene detection. METHODS: Peripheral blood samples of 166 thalassemia gene test subjects were collected and tested for thalassemia genes by microarray chip method and Gap-PCR method combined with PCR-reverse dot blot hybridization method according to double-blind control test. The specificity, sensitivity, positive predictive value, negative predictive value, and total coincidence rate of the microarray chip method were evaluated. When the two methods were inconsistent, multiplex ligation dependent probe amplification (MLPA) was used to verify the deletional α-thalassemia. RESULTS: Compared with Gap-PCR method, specificity, sensitivity, positive predictive value, negative predictive value, Youden index, and total coincidence rate of microarray chip method was 100% (70/70), 96.88% (93/96), 100% (93/93), 95.89% (70/73), 0.969, and 97.59% (162/166), respectively, while compared with PCR-reverse dot blot hybridization method was 100% (125/125), 100% (41/41), 100% (41/41), 100% (125/125), 1, and 100% (166/166), respectively. CONCLUSION: The microarray chip method for α-thalassemia gene detection shows the advantages of high specificity, sensitivity, and throughput.

2.
J Am Chem Soc ; 143(47): 19769-19777, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34788033

RESUMO

Artificial intelligence (AI) based self-learning or self-improving material discovery system will enable next-generation material discovery. Herein, we demonstrate how to combine accurate prediction of material performance via first-principle calculations and Bayesian optimization-based active learning to realize a self-improving discovery system for high-performance photosensitizers (PSs). Through self-improving cycles, such a system can improve the model prediction accuracy (best mean absolute error of 0.090 eV for singlet-triplet spitting) and high-performance PS search ability, realizing efficient discovery of PSs. From a molecular space with more than 7 million molecules, 5357 potential high-performance PSs were discovered. Four PSs were further synthesized to show performance comparable with or superior to commercial ones. This work highlights the potential of active learning in first-principle-based materials design, and the discovered structures could boost the development of photosensitization related applications.

3.
Adv Sci (Weinh) ; : e2101074, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34821473

RESUMO

For practical applications, molecules often exist in an aggregate state. Therefore, it is of great value if one can predict the performance of molecules when forming aggregates, for example, aggregation-induced emission (AIE) or aggregation-caused quenching (ACQ). Herein, a database containing AIE/ACQ molecules reported in the literature is first established. Through training, these machine learning (ML) models can build up the structure-property relationship and thus implement fast prediction of AIE/ACQ properties. To this end, a multi-modal approach is proposed, multiple prediction methods are compared and designed, and thus an ensemble strategy is developed. First, multiple molecular descriptors are considered at the same time, major features are extracted by dimensionality reduction, and multi-modal features are synthesized. Then, several state-of-the-art methods are designed and compared to analyze the advantages of the different methods. Finally, the ensemble strategy combines the advantages of the multiple methods to obtain the final prediction result. The reliability of this approach in an unknown molecular space is further verified by three newly designed molecules. Reasonable consistency between model predictions and experimental outcomes is obtained. The result indicates that ML can be a powerful tool to predict molecular properties in the aggregated state, thus accelerating the development of solid-state optical materials.

5.
Curr Mol Med ; 2021 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-34749620

RESUMO

BACKGROUND: MicroRNAs (miRNAs) are about 22-nucleotide, small, non-coding RNAs that control gene expression post-transcriptionally. Helminth parasites usually express a unique repertoire of genes, including miRNAs, across different developmental stages with subtle regulatory mechanisms. OBJECTIVE: There is a necessity to investigate the involvement of miRNAs in the development of parasites, host-parasite interaction, immune evasion and their abilities to govern infection in hosts. miRNAs present in helminth parasites have been summarized in the current systematic review (SR). METHODS: Electronic databases, including PubMed, Scopus, ProQuest, Embase, and Google Scholar search engine, were searched to identify helminth miRNA studies published from February 1993 till December 2019. Only the published articles in English were included in the study. RESULTS: A total of 1769 articles were preliminarily recorded. Following the strict inclusion and exclusion criteria, 105 studies were included in this SR. Most of these studies focused on the identification of miRNAs in helminth parasites and/or probing of differentially expressed host miRNA profiles in specific relevant tissues, while 12 studies aimed to detect parasite-derived miRNAs in host circulating system and 15 studies characterized extracellular vesicles (EV)-derived miRNAs secreted by parasites. CONCLUSION: In the current SR, information regarding all miRNAs expressed in helminth parasites has been comprehensively provided and the utility of helminth parasites-derived miRNAs in diagnosis and control of parasitic infections has been discussed. Furthermore, functional studies on helminth-derived miRNAs have also been presented.

6.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(5): 1561-1565, 2021 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-34627440

RESUMO

OBJECTIVE: To perform dried blood spots thalassemia gene detection in patients with positive blood phenotypes by microarray technology, and evaluate its value in clinical detection. METHODS: DNA samples were extracted from dried blood spots of 410 patients. Microarray technology was used to detect 3 deletion and 3 non-deletion types of α-thalassemia and 19 ß-thalassemia point mutations which were common gene mutions in China. RESULTS: There were 357 positive cases in all the 410 tested samples with the positive rate 87.07%, among which 299 cases (72.93%) carried deletion or point mutations of α-thalassemia, 29 cases (7.07%) carried point mutations of ß-thalassemia and 29 cases (7.07%) carried gene mutations of complex αß-thalassemia syndrome. The mutations of α-thalassemia were involved with --SEA heterozygous deletion (177 cases, 59.2%), αCS heterozygote (60 cases, 20.07%) and several other genotypes. The common mutations of ß- thalassemia were involved with ßCD41-42 heterozygote (10 cases, 34.48%) and ßCD17 heterozygote (9 cases, 31.03%). The mutations of complex αß-thalassemia syndrome were mainly involved with --SEA/αα+ßCD17/ßN (7 cases, 24.14%), αCSα/αα + ßCD41-42/ßN (3 cases, 10.34%) and -α4.2/αα + ßCD17/ßN (3 cases, 10.34%). CONCLUSION: The most common genetic mutations are --SEA for α-thalassemia and CD41-42 for ß-thalassemia in Liuzhou, Guangxi Zhuang Autonomous Region. A and ß-thalassemia can be detected at the same time by microarray chip technology in a high throughput manner.


Assuntos
Talassemia alfa , Talassemia beta , China , Humanos , Mutação , Análise de Sequência com Séries de Oligonucleotídeos , Talassemia alfa/genética , Talassemia beta/genética
7.
Nanomaterials (Basel) ; 11(10)2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34684991

RESUMO

Graphene-reinforced aluminum matrix composites (GRAMCs) attract great interest in industries due to their high performance potential. High-temperature processes such as sintering and aging are usually applied during the preparation of GRAMCs, leading to grain coarsening that significantly influences its properties. In this work, a modified 3D Monte Carlo Potts model was proposed to investigate the effect of content and size of graphene on the grain evolution during the heat treatment of GRAMCs. Grain growth with graphene contents from 0.5 wt.% to 4.5 wt.% and sizes from 5 µm to 15 µm were simulated. The grain growth process, final grain size and morphology of the microstructure were predicted. The results indicated that both the content and size of the reinforcements had an impact on the grain evolution. The pinning effect of grain size can be enhanced by increasing the content and decreasing the size of graphene. Agglomeration and self-contacting phenomena of the graphene arose obviously when the contents and sizes were relatively high. The average grain size decreased by 48.77% when the content increased from 0.5 wt.% to 4.5 wt.%. The proposed method and predicted regulations can provide a reference for the design and fabrication of GRAMCs.

8.
Cell Rep ; 37(1): 109793, 2021 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-34587478

RESUMO

The mortality risk of coronavirus disease 2019 (COVID-19) patients has been linked to the cytokine storm caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Understanding the inflammatory responses shared between COVID-19 and other infectious diseases that feature cytokine storms may therefore help in developing improved therapeutic strategies. Here, we use integrative analysis of single-cell transcriptomes to characterize the inflammatory signatures of peripheral blood mononuclear cells from patients with COVID-19, sepsis, and HIV infection. We identify ten hyperinflammatory cell subtypes in which monocytes are the main contributors to the transcriptional differences in these infections. Monocytes from COVID-19 patients share hyperinflammatory signatures with HIV infection and immunosuppressive signatures with sepsis. Finally, we construct a "three-stage" model of heterogeneity among COVID-19 patients, related to the hyperinflammatory and immunosuppressive signatures in monocytes. Our study thus reveals cellular and molecular insights about inflammatory responses to SARS-CoV-2 infection and provides therapeutic guidance to improve treatments for subsets of COVID-19 patients.


Assuntos
COVID-19/sangue , COVID-19/imunologia , Infecções por HIV/sangue , Leucócitos Mononucleares/metabolismo , SARS-CoV-2/imunologia , Sepse/sangue , Transcriptoma , COVID-19/virologia , Síndrome da Liberação de Citocina/sangue , Síndrome da Liberação de Citocina/imunologia , Citocinas/sangue , Análise de Dados , Conjuntos de Dados como Assunto , Infecções por HIV/imunologia , HIV-1/imunologia , Humanos , Inflamação/sangue , Leucócitos Mononucleares/imunologia , Sepse/imunologia , Análise de Célula Única
9.
Infect Dis Poverty ; 10(1): 121, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34556183

RESUMO

BACKGROUND: Zoonotic schistosomiasis, caused by Schistosoma japonicum, remains a major public health problem in the Philippines. This study aimed to evaluate the commercially available rapid diagnostic point-of-care circulating cathodic antigen (POC-CCA) test in detecting individuals infected with S. japonicum in a human cohort from an endemic area for schistosomiasis japonica in the Philippines. METHODS: Clinical samples were collectedin 18 barangays endemic for S. japonicum infection in Laoang and Palapag municipalities, Northern Samar, the Philippines, in 2015. The presence of CCA in filter-concentrated urine samples (n = 412) was evaluated using the commercial kits and the results were converted to images, which were further analyzed by ImageJ software to calculate R values. The diagnostic performance of the immunochromatographic POC-CCA test was compared using the Kato-Katz (KK) procedure, in-house enzyme-linked immunosorbent assays (ELISAs) and droplet digital (dd) PCR assays as reference. RESULTS: The POC-CCA test was able to detect S. japonicum-infected individuals in the cohort with an eggs per gram of faeces (EPG) more than or equal to 10 with sensitivity/specificity values of 63.3%/93.3%. However, the assay showed an inability to diagnose schistosomiasis japonica infections in all cohort KK-positive individuals, of which the majority had an extremely low egg burden (EPG: 1-9). The prevalence of S. japonicum infection in the total cohort determined by the POC-CCA test was 12.4%, only half of that determined by the KK method (26.2%). When compared with the ELISAs and ddPCR assays as a reference, the POC-CCA assay was further shown to be a test with low sensitivity. Nevertheless, the assay exhibited significant positive correlations with egg burden determined by the KK technique and the target gene copy number index values determined by the ddPCR assays within the entire cohort. CONCLUSIONS: By using in silico image analysis, the POC-CCA cassette test could be converted to a quantitative assay to avoid reader-variability. Because of its low sensitivity, the commercially available POC-CCA assay had limited potential for determining the status of a S. japonicum infection in the target cohort. The assay should be applied with caution in populations where schistosome parasites (especially S. japonicum) are present at low infection intensity.


Assuntos
Antígenos de Helmintos/sangue , Fezes/parasitologia , Sistemas Automatizados de Assistência Junto ao Leito , Schistosoma japonicum/isolamento & purificação , Esquistossomose Japônica/diagnóstico , Testes Sorológicos/métodos , Animais , Anti-Helmínticos/uso terapêutico , Antígenos de Helmintos/análise , Ensaio de Imunoadsorção Enzimática , Humanos , Doenças Negligenciadas/epidemiologia , Filipinas/epidemiologia , Reação em Cadeia da Polimerase , Prevalência , Schistosoma japonicum/imunologia , Esquistossomose Japônica/tratamento farmacológico , Esquistossomose Japônica/epidemiologia , Sensibilidade e Especificidade
10.
Front Cell Infect Microbiol ; 11: 708952, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34249784

RESUMO

As an adaption to their complex lifecycles, helminth parasites garner a unique repertoire of genes at different developmental stages with subtle regulatory mechanisms. These parasitic worms release differential components such as microRNAs (miRNAs) and extracellular vesicles (EVs) as mediators which participate in the host-parasite interaction, immune regulation/evasion, and in governing processes associated with host infection. MiRNAs are small (~ 22-nucleotides) non-coding RNAs that regulate gene expression at the post-transcriptional level, and can exist in stable form in bodily fluids such as serum/plasma, urine, saliva and bile. In addition to reports focusing on the identification of miRNAs or in the probing of differentially expressed miRNA profiles in different development stages/sexes or in specific tissues, a number of studies have focused on the detection of helminth-derived miRNAs in the mammalian host circulatory system as diagnostic biomarkers. Extracellular vesicles (EVs), small membrane-surrounded structures secreted by a wide variety of cell types, contain rich cargos that are important in cell-cell communication. EVs have attracted wide attention due to their unique functional relevance in host-parasite interactions and for their potential value in translational applications such as biomarker discovery. In the current review, we discuss the status and potential of helminth parasite-derived circulating miRNAs and EV cargos as novel diagnostic tools.


Assuntos
Vesículas Extracelulares , Helmintos , MicroRNAs , Parasitos , Animais , Biomarcadores , Helmintos/genética , MicroRNAs/genética
11.
BMC Biol ; 19(1): 79, 2021 04 16.
Artigo em Inglês | MEDLINE | ID: mdl-33863328

RESUMO

BACKGROUND: Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease that involves a variety of cell types. However, how the epigenetic dysregulations of peripheral immune cells contribute to the pathogenesis of RA still remains largely unclear. RESULTS: Here, we analysed the genome-wide active DNA regulatory elements of four major immune cells, namely monocytes, B cells, CD4+ T cells and CD8+ T cells, in peripheral blood of RA patients, osteoarthritis (OA) patients and healthy donors using Assay of Transposase Accessible Chromatin with sequencing (ATAC-seq). We found a strong RA-associated chromatin dysregulation signature in monocytes, but no other examined cell types. Moreover, we found that serum C-reactive protein (CRP) can induce the RA-associated chromatin dysregulation in monocytes via in vitro experiments. And the extent of this dysregulation was regulated through the transcription factor FRA2. CONCLUSIONS: Together, our study revealed a CRP-induced pathogenic chromatin dysregulation signature in monocytes from RA patients and predicted the responsible signalling pathway as potential therapeutic targets for the disease.

12.
Brief Bioinform ; 22(5)2021 09 02.
Artigo em Inglês | MEDLINE | ID: mdl-33834202

RESUMO

The low capture rate of expressed RNAs from single-cell sequencing technology is one of the major obstacles to downstream functional genomics analyses. Recently, a number of imputation methods have emerged for single-cell transcriptome data, however, recovering missing values in very sparse expression matrices remains a substantial challenge. Here, we propose a new algorithm, WEDGE (WEighted Decomposition of Gene Expression), to impute gene expression matrices by using a biased low-rank matrix decomposition method. WEDGE successfully recovered expression matrices, reproduced the cell-wise and gene-wise correlations and improved the clustering of cells, performing impressively for applications with sparse datasets. Overall, this study shows a potent approach for imputing sparse expression matrix data, and our WEDGE algorithm should help many researchers to more profitably explore the biological meanings embedded in their single-cell RNA sequencing datasets. The source code of WEDGE has been released at https://github.com/QuKunLab/WEDGE.


Assuntos
Algoritmos , Biologia Computacional/métodos , Perfilação da Expressão Gênica/métodos , RNA-Seq/métodos , Análise de Célula Única/métodos , COVID-19/sangue , COVID-19/genética , COVID-19/virologia , Análise por Conglomerados , Simulação por Computador , Genômica/métodos , Humanos , Leucócitos Mononucleares/classificação , Leucócitos Mononucleares/metabolismo , Reprodutibilidade dos Testes , SARS-CoV-2/fisiologia , Índice de Gravidade de Doença
13.
Cell Biol Int ; 45(7): 1468-1476, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33675274

RESUMO

Forkhead box O1 (FOXO1) is a key regulator of osteogenesis. The aim of this study was to identify the mechanisms of microRNAs (miRNAs) targeting FOXO1 in osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). Three miRNA target prediction programs were used to search for potential miRNAs that target FOXO1. Quantitative real-time polymerase chain reaction was conducted to detect the expression of miR-1271-5p and FOXO1 during osteogenic differentiation. Target gene prediction and screening, luciferase reporter assay was used to verify the downstream target gene of miR-1271-5p. The expression levels of FOXO1 and Runx2 were detected by RT-qPCR and Western blot analysis. Alkaline phosphatase (ALP) activity and matrix mineralization were detected by biochemical methods. The expression levels of Runx2, ALP, and osteocalcin were detected by RT-qPCR. Our results showed that miR-1271-5p was downregulated during osteogenic induction. And the expression levels of miR-1271-5p were higher in osteoporotic tissues than that in adjacent nonosteoporotic tissues. The expression levels of FOXO1 were lower in osteoporotic tissues than that in adjacent nonosteoporotic tissues. And a negative correlation was found between miR-1271-5p and FOXO1 in osteoporotic tissues. Overexpression of miR-1271-5p downregulated FOXO1 and inhibited osteogenic differentiation in hMSCs. Overexpression of miR-1271-5p downregulated the expression of osteogenic markers and reduced ALP activity. In addition, ectopic expression of FOXO1 reversed the effect of miR-1271-5p on osteogenic differentiation. In conclusion, miR-1271-5p functioned as a therapeutic target of osteogenic differentiation in hMSCs by inhibiting FOXO1, which provides valuable insights into the use of miR-1271-5p as a target in the treatment of osteoporosis and other bone metabolic diseases.

14.
Cell ; 184(7): 1895-1913.e19, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-33657410

RESUMO

A dysfunctional immune response in coronavirus disease 2019 (COVID-19) patients is a recurrent theme impacting symptoms and mortality, yet a detailed understanding of pertinent immune cells is not complete. We applied single-cell RNA sequencing to 284 samples from 196 COVID-19 patients and controls and created a comprehensive immune landscape with 1.46 million cells. The large dataset enabled us to identify that different peripheral immune subtype changes are associated with distinct clinical features, including age, sex, severity, and disease stages of COVID-19. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was found in diverse epithelial and immune cell types, accompanied by dramatic transcriptomic changes within virus-positive cells. Systemic upregulation of S100A8/A9, mainly by megakaryocytes and monocytes in the peripheral blood, may contribute to the cytokine storms frequently observed in severe patients. Our data provide a rich resource for understanding the pathogenesis of and developing effective therapeutic strategies for COVID-19.


Assuntos
COVID-19/imunologia , Megacariócitos/imunologia , Monócitos/imunologia , RNA Viral , SARS-CoV-2/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , China , Estudos de Coortes , Citocinas/metabolismo , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , RNA Viral/sangue , RNA Viral/isolamento & purificação , Análise de Célula Única , Transcriptoma/imunologia , Adulto Jovem
15.
Genome Med ; 13(1): 49, 2021 03 26.
Artigo em Inglês | MEDLINE | ID: mdl-33771202

RESUMO

BACKGROUND: T cells generated from thymopoiesis are essential for the immune system, and recent single-cell studies have contributed to our understanding of the development of thymocytes at the genetic and epigenetic levels. However, the development of double-positive (DP) T cells, which comprise the majority of thymocytes, has not been well investigated. METHODS: We applied single-cell sequencing to mouse thymocytes and analyzed the transcriptome data using Seurat. By applying unsupervised clustering, we defined thymocyte subtypes and validated DP cell subtypes by flow cytometry. We classified the cell cycle phases of each cell according to expression of cell cycle phase-specific genes. For immune synapse detection, we used immunofluorescent staining and ImageStream-based flow cytometry. We studied and integrated human thymocyte data to verify the conservation of our findings and also performed cross-species comparisons to examine species-specific gene regulation. RESULTS: We classified blast, rearrangement, and selection subtypes of DP thymocytes and used the surface markers CD2 and Ly6d to identify these subtypes by flow cytometry. Based on this new classification, we found that the proliferation of blast DP cells is quite different from that of double-positive cells and other cell types, which tend to exit the cell cycle after a single round. At the DP cell selection stage, we observed that CD8-associated immune synapses formed between thymocytes, indicating that CD8sp selection occurred among thymocytes themselves. Moreover, cross-species comparison revealed species-specific transcription factors (TFs) that contribute to the transcriptional differences of thymocytes from humans and mice. CONCLUSIONS: Our study classified DP thymocyte subtypes of different developmental stages and provided new insight into the development of DP thymocytes at single-cell resolution, furthering our knowledge of the fundamental immunological process of thymopoiesis.

16.
Front Immunol ; 12: 619776, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33692793

RESUMO

Parasitic helminths, comprising the flatworms (tapeworms and flukes) and nematodes (roundworms), have plagued humans persistently over a considerable period of time. It is now known that the degree of exposure to these and other pathogens inversely correlates with the incidence of both T helper 1 (Th1)-mediated autoimmunity and Th2-mediated allergy. Accordingly, there has been recent increased interest in utilizing active helminth worm infections and helminth-derived products for the treatment of human autoimmune and inflammatory diseases and to alleviate disease severity. Indeed, there is an accumulating list of novel helminth derived molecules, including proteins, peptides, and microRNAs, that have been shown to exhibit therapeutic potential in a variety of disease models. Here we consider the blood-dwelling schistosome flukes, which have evolved subtle immune regulatory mechanisms that promote parasite survival but at the same time minimize host tissue immunopathology. We review and discuss the recent advances in using schistosome infection and schistosome-derived products as therapeutics to treat or mitigate human immune-related disorders, including allergic asthma, arthritis, colitis, diabetes, sepsis, cystitis, and cancer.


Assuntos
Produtos Biológicos/farmacologia , Doenças do Sistema Imunitário/tratamento farmacológico , Fatores Imunológicos/farmacologia , Schistosoma/metabolismo , Animais , Produtos Biológicos/química , Produtos Biológicos/uso terapêutico , Gerenciamento Clínico , Modelos Animais de Doenças , Humanos , Doenças do Sistema Imunitário/diagnóstico , Doenças do Sistema Imunitário/etiologia , Fatores Imunológicos/química , Fatores Imunológicos/uso terapêutico , Imunomodulação/efeitos dos fármacos , Camundongos , Proteínas Recombinantes/farmacologia , Proteínas Recombinantes/uso terapêutico , Schistosoma/química
17.
Medicine (Baltimore) ; 100(3): e23922, 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33545963

RESUMO

BACKGROUND: This meta-analysis was conducted in order to understand the clinical efficacy of stent insertion with high-intensity focused ultrasound (HIFU) ablation for the treatment of malignant biliary obstruction (MBO). METHODS: The Pubmed, Embase, and Cochrane Library databases were searched for all relevant studies published through July 2020. The meta-analysis was conducted using RevMan v5.3, with analyzed study endpoints including the rate of stent dysfunction, time to stent dysfunction, stent patency, complication rate, and overall survival (OS). RESULTS: In total, 35 potentially relevant studies were initially identified, of which 6 were ultimately included in the present meta-analysis. These 6 studies included 429 MBO patients that were treated either only via stenting (n = 221) or via stenting in combination with HIFU ablation (n = 208). Pooled stent dysfunction rates in the stent and stent with HIFU groups were 25.9% and 18.0%, respectively (OR: 1.59; 95% CI: 0.88, 2.84, P = .12). The average time to stent dysfunction was significantly longer in the stent with HIFU group relative to the stent group (MD: -3.15; 95% CI: -3.53, -2.77, P < .0001). Pooled complication rates in the stent and stent with HIFU groups were 17.1% and 19.6%, respectively (OR: 0.88; 95% CI: 0.49, 1.58, P = .67). Stent patency and OS were both significantly longer in the stent with HIFU group relative to the stent group (P < .0001 and.0001, respectively). Funnel plot analyses did not reveal any significant evidence of publication bias linked to the selected study endpoints. CONCLUSIONS: This meta-analysis found that a combined stenting and HIFU ablation approach can achieve better stent patency and OS in MBO patients relative to stent insertion alone.


Assuntos
Neoplasias do Sistema Biliar/cirurgia , Colestase/cirurgia , Ablação por Ultrassom Focalizado de Alta Intensidade , Stents , Neoplasias do Sistema Biliar/complicações , Colestase/complicações , Humanos , Metanálise como Assunto , Revisões Sistemáticas como Assunto , Resultado do Tratamento
18.
Cell Discov ; 7(1): 1, 2021 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-33390590

RESUMO

Maintaining homeostasis of the decidual immune microenvironment at the maternal-fetal interface is essential for placentation and reproductive success. Although distinct decidual immune cell subpopulations have been identified under normal conditions, systematic understanding of the spectrum and heterogeneity of leukocytes under recurrent miscarriage in human deciduas remains unclear. To address this, we profiled the respective transcriptomes of 18,646 primary human decidual immune cells isolated from patients with recurrent pregnancy loss (RPL) and healthy controls at single-cell resolution. We discovered dramatic differential distributions of immune cell subsets in RPL patients compared with the normal decidual immune microenvironment. Furthermore, we found a subset of decidual natural killer (NK) cells that support embryo growth were diminished in proportion due to abnormal NK cell development in RPL patients. We also elucidated the altered cellular interactions between the decidual immune cell subsets in the microenvironment and those of the immune cells with stromal cells and extravillous trophoblast under disease state. These results provided deeper insights into the RPL decidual immune microenvironment disorder that are potentially applicable to improve the diagnosis and therapeutics of this disease.

19.
Ann Palliat Med ; 10(1): 250-257, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33474963

RESUMO

BACKGROUND: Advanced non-small cell lung cancer (NSCLC) patients receiving chemotherapy usually experience adverse events, especially chemotherapy-induced nausea and vomiting (CINV), which reduces their quality of life (QoL). The present before and after study was performed to investigate the influence of graded nursing on adverse events and QoL of advanced NSCLC patients. METHODS: Patients with stage III or IV NSCLC who received conventional nursing from January 2017 to December 2017 were selected as the control group. Patients with stage III or IV NSCLC who received staged nursing from January 2018 to December 2018 were selected as the study group. Adverse events, especially CINV, were recorded as a primary outcome of this study. The QoL of patients was assessed using the European Organization for Research and Treatment of Cancer (EORTC) quality of life questionnaire (QLQ)-C30 scale. All patients were followed up for at least 1 year. RESULTS: Patients showed no significant difference in baseline clinical characteristics between the control and study groups. Adverse events, including vomiting, nausea, constipation, fever, cough, mucositis, and epigastric pain, occurred, of which CINV was the most common. However, no difference in these adverse events was found between the 2 groups. The EORTC QLQ-C30 scale, which assesses the physical, role, emotional, cognitive, and social functions and global health status, indicated no significant difference between the 2 groups prior to receiving chemotherapy. Expectedly, patients in the study group had improved emotional, cognitive, and social function and global health status compared with the control group (P=0.004, P=0.017, P=0.008, and P=0.003, respectively). Additionally, the Kaplan-Meier survival curve indicated that patients in the study group had a statistically better survival rate than patients in the control group (P=0.032). Graded nursing was found to be a protective factor of overall survival of advanced NSCLC patients according to the Cox proportional hazards model (P=0.024). CONCLUSIONS: Graded nursing could significantly improve QoL and prolong the survival of advanced NSCLC patients receiving chemotherapy, and may be a feasible measurement to improve their prognosis.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Protocolos de Quimioterapia Combinada Antineoplásica , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Qualidade de Vida , Inquéritos e Questionários , Taxa de Sobrevida
20.
J Colloid Interface Sci ; 588: 637-645, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33267956

RESUMO

Supercapacitors are deemed as reliable power sources for portable devices and electric vehicles. Electrode materials with high energy and power densities are greatly needed. Herein, we designed reduced-graphene-oxide supported nickel-cobalt layered double hydroxide nanosheets (NiCo-LDH/rGO) as electrode materials. The introduction of graphene could largely enhance the conductivity, and the supported NiCo-LDH could effectively prevent graphene from self-aggregation. Thanks to the synergistic effect of conductive graphene and electro-active LDH, the nanocomposites delivered a capacitance of 1675 F g-1 at 1 A g-1 and decent rate performance (capacitance retention of 83.8% at 10 A g-1); while NiCo-LDH could only exhibited a capacitance of 920 F g-1 at 1 A g-1 and 81.5% of the capacitance remained at 10 A g-1. The asymmetric supercapacitors assembled with NiCo-LDH/rGO and activated carbon (AC) delivered high energy density and power density, up to 49.9 Wh kg-1 and 3747.9 W kg-1, respectively. The appealing electrochemical performance indicates its huge application potential in supercapacitors.

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