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1.
Biochim Biophys Acta Proteins Proteom ; 1871(3): 140889, 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36610583

RESUMO

Metabolic stability of proteins plays a vital role in various dedicated cellular processes. Traditional methods of measuring the metabolic stability are time-consuming and expensive. Therefore, we developed a more efficient computational approach to understand the protein dynamic action mechanisms in biological process networks. In this study, we collected 341 short-lived proteins and 824 non-short-lived proteins from U2OS; 342 short-lived proteins and 821 non-short-lived proteins from HEK293T; 424 short-lived proteins and 1153 non-short-lived proteins from HCT116; and 384 short-lived proteins and 992 non-short-lived proteins from RPE1. The proteins were encoded by GO and KEGG enrichment scores based on the genes and their neighbors in STRING, resulting in 20,681 GO term features and 297 KEGG pathway features. We also incorporated the protein interaction information from STRING into the features and obtained 19,247 node features. Boruta and mRMR methods were used for feature filtering, and IFS method was used to obtain the best feature subsets and create the models with the highest performance. The present study identified 42 features that did not appear in previous studies and classified them into eight groups according to their functional annotation. By reviewing the literature, we found that the following three functional groups were critical in determining the stability of proteins: synaptic transmission, post-translational modifications, and cell fate determination. These findings may serve as a valuable reference for developing drugs that target protein stability.

2.
BMC Biol ; 21(1): 11, 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36690984

RESUMO

BACKGROUND: Insects live in almost every habitat on earth. To adapt to their diverse environments, insects have developed a myriad of different strategies for reproduction reflected in diverse anatomical and behavioral features that the reproductive systems of females exhibit. Yet, ovarian development remains largely uncharacterized in most species except Drosophila melanogaster (D. melanogaster), a high Diptera model. In this study, we investigated the detailed developmental process of the ovary in Aedes aegypti (Ae. aegypti), a major vector of various disease-causing pathogens that inhabits tropical and subtropical regions. RESULTS: Compared with Drosophila melanogaster, a model of higher Diptera, the processes of pole cell formation and gonad establishment during embryonic stage are highly conserved in Ae. aegypti. However, Ae. aegypti utilizes a distinct strategy to form functional ovaries during larval/pupal development. First, during larval stage, Ae. aegypti primordial germ cells (PGCs) undergo a cyst-like proliferation with synchronized divisions and incomplete cytokinesis, leading to the formation of one tightly packed "PGC mass" containing several interconnected cysts, different from D. melanogaster PGCs that divide individually. This cyst-like proliferation is regulated by the target of rapamycin (TOR) pathway upon nutritional status. Second, ecdysone-triggered ovariole formation during metamorphosis exhibits distinct events, including "PGC mass" breakdown, terminal filament cell degeneration, and pre-ovariole migration. These unique developmental features might explain the structural and behavioral differences between Aedes and Drosophila ovaries. Importantly, both cyst-like proliferation and distinct ovariole formation are also observed in Culex quinquefasciatus and Anopheles sinensis, suggesting a conserved mode of ovarian development among mosquito species. In comparison with Drosophila, the ovarian development in Aedes and other mosquitoes might represent a primitive mode in the lower Diptera. CONCLUSIONS: Our study reveals a new mode of ovarian development in mosquitoes, providing insights into a better understanding of the reproductive system and evolutionary relationship among insects.


Assuntos
Aedes , Culex , Animais , Feminino , Drosophila melanogaster , Mosquitos Vetores , Larva , Drosophila
3.
Adv Sci (Weinh) ; : e2205395, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36594618

RESUMO

Breast cancer is now the most frequently diagnosed malignancy, and metastasis remains the leading cause of death in breast cancer. However, little is known about the dynamic changes during the evolvement of dissemination. In this study, 65 968 cells from four patients with breast cancer and paired metastatic axillary lymph nodes are profiled using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics. A disseminated cancer cell cluster with high levels of oxidative phosphorylation (OXPHOS), including the upregulation of cytochrome C oxidase subunit 6C and dehydrogenase/reductase 2, is identified. The transition between glycolysis and OXPHOS when dissemination initiates is noticed. Furthermore, this distinct cell cluster is distributed along the tumor's leading edge. The findings here are verified in three different cohorts of breast cancer patients and an external scRNA-seq dataset, which includes eight patients with breast cancer and paired metastatic axillary lymph nodes. This work describes the dynamic metabolic evolvement of early disseminated breast cancer and reveals a switch between glycolysis and OXPHOS in breast cancer cells as the early event during lymph node metastasis.

4.
Nat Commun ; 14(1): 315, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36658155

RESUMO

N6-methyladenosine (m6A) has been demonstrated to regulate RNA metabolism and various biological processes, including gametogenesis and embryogenesis. However, the landscape and function of m6A at single cell resolution have not been extensively studied in mammalian oocytes or during pre-implantation. In this study, we developed a single-cell m6A sequencing (scm6A-seq) method to simultaneously profile the m6A methylome and transcriptome in single oocytes/blastomeres of cleavage-stage embryos. We found that m6A deficiency leads to aberrant RNA clearance and consequent low quality of Mettl3Gdf9 conditional knockout (cKO) oocytes. We further revealed that m6A regulates the translation and stability of modified RNAs in metaphase II (MII) oocytes and during oocyte-to-embryo transition, respectively. Moreover, we observed m6A-dependent asymmetries in the epi-transcriptome between the blastomeres of two-cell embryo. scm6A-seq thus allows in-depth investigation into m6A characteristics and functions, and the findings provide invaluable single-cell resolution resources for delineating the underlying mechanism for gametogenesis and early embryonic development.


Assuntos
Oócitos , Oogênese , Animais , Oócitos/metabolismo , Desenvolvimento Embrionário/genética , Transcriptoma/genética , RNA/metabolismo , Mamíferos/genética
5.
Bioeng Transl Med ; 8(1): e10364, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36684070

RESUMO

Cartilage regeneration after injury is still a great challenge in clinics, which suffers from its avascularity and poor proliferative ability. Herein we designed a novel biocompatible cellulose nanocrystal/GelMA (gelatin-methacrylate anhydride)/HAMA (hyaluronic acid-methacrylate anhydride)-blended hydrogel scaffold system, loaded with synthetic melanin nanoparticles (SMNP) and a bioactive drug kartogenin (KGN) for theranostic purpose. We found that the SMNP-KGN/Gel showed favorable mechanical property, thermal stability, and distinct magnetic resonance imaging (MRI) contrast enhancement. Meanwhile, the sustained release of KGN could recruit bone-derived mesenchymal stem cells to proliferate and differentiate into chondrocytes, which promoted cartilage regeneration in vitro and in vivo. The hydrogel degradation and cartilage restoration were simultaneously monitored by multiparametric MRI for 12 weeks, and further confirmed by histological analysis. Together, these results validated the multifunctional hydrogel as a promising tissue engineering platform for noninvasive imaging-guided precision therapy in cartilage regenerative medicine.

6.
Cell Rep ; 42(1): 112007, 2023 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-36641752

RESUMO

Maintenance of stem cells requires the concerted actions of niche-derived signals and stem cell-intrinsic factors. Although Decapentaplegic (Dpp), a Drosophila bone morphogenetic protein (BMP) molecule, can act as a long-range morphogen, its function is spatially limited to the germline stem cell niche in the germarium. We show here that Integrator, a complex known to be involved in RNA polymerase II (RNAPII)-mediated transcriptional regulation in the nucleus, promotes germline differentiation by restricting niche-derived Dpp/BMP activity in the cytoplasm. Further results show that Integrator works in various developmental contexts to desensitize the cellular response to Dpp/BMP signaling during Drosophila development. Mechanistically, our results show that Integrator forms a multi-subunit complex with the type I receptor Thickveins (Tkv) and other Dpp/BMP signaling components and acts in a negative feedback loop to promote Tkv turnover independent of its transcriptional activity. Similarly, human Integrator subunits bind transforming growth factor ß (TGF-ß)/BMP signaling components and antagonize their activity, suggesting a conserved role of Integrator across metazoans.

7.
Int J Mol Sci ; 24(2)2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36675143

RESUMO

Camptothecin (CPT), first isolated from Chinese tree Camptotheca acuminate, produces rapid and prolonged inhibition of DNA synthesis and induction of DNA damage by targeting topoisomerase I (top1), which is highly activated in cancer cells. CPT thus exhibits remarkable anticancer activities in various cancer types, and is a promising therapeutic agent for the treatment of cancers. However, it remains to be uncovered underlying its cytotoxicity toward germ cells. In this study we found that CPT, a cell cycle-specific anticancer agent, reduced fecundity and exhibited significant cytotoxicity toward GSCs and two-cell cysts. We showed that CPT induced GSC loss and retarded two-cell cysts differentiation in a niche- or apoptosis-independent manner. Instead, CPT induced ectopic expression of a differentiation factor, bag of marbles (Bam), and regulated the expression of cyclin A, which contributed to GSC loss. In addition, CPT compromised two-cell cysts differentiation by decreasing the expression of Bam and inducing cell arrest at G1/S phase via cyclin A, eventually resulting in two-cell accumulation. Collectively, this study demonstrates, for the first time in vivo, that the Bam-cyclin A axis is involved in CPT-mediated germline stem cell loss and two-cell cysts differentiation defects via inducing cell cycle arrest, which could provide information underlying toxicological effects of CPT in the productive system, and feature its potential to develop as a pharmacology-based germline stem cell regulation agent.


Assuntos
Cistos , Proteínas de Drosophila , Animais , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Ciclinas/metabolismo , Proteínas de Drosophila/metabolismo , Diferenciação Celular , Ciclina A/metabolismo , Camptotecina/farmacologia , Camptotecina/metabolismo , Pontos de Checagem do Ciclo Celular , Células Germinativas/metabolismo , Cistos/metabolismo
8.
Biomed Res Int ; 2023: 5333361, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36644165

RESUMO

Long-term cigarette smoking causes various human diseases, including respiratory disease, cancer, and gastrointestinal (GI) disorders. Alterations in gene expression and variable splicing processes induced by smoking are associated with the development of diseases. This study applied advanced machine learning methods to identify the isoforms with important roles in distinguishing smokers from former smokers based on the expression profile of isoforms from current and former smokers collected in one previous study. These isoforms were deemed as features, which were first analyzed by the Boruta to select features highly correlated with the target variables. Then, the selected features were evaluated by four feature ranking algorithms, resulting in four feature lists. The incremental feature selection method was applied to each list for obtaining the optimal feature subsets and building high-performance classification models. Furthermore, a series of classification rules were accessed by decision tree with the highest performance. Eventually, the rationality of the mined isoforms (features) and classification rules was verified by reviewing previous research. Features such as isoforms ENST00000464835 (expressed by LRRN3), ENST00000622663 (expressed by SASH1), and ENST00000284311 (expressed by GPR15), and pathways (cytotoxicity mediated by natural killer cell and cytokine-cytokine receptor interaction) revealed by the enrichment analysis, were highly relevant to smoking response, suggesting the robustness of our analysis pipeline.


Assuntos
Fumar , Transcriptoma , Humanos , Algoritmos , Aprendizado de Máquina , Receptores Acoplados a Proteínas G/genética , Receptores de Peptídeos/genética , Fumar/efeitos adversos , Fumar/genética , Transcriptoma/genética
10.
Medicine (Baltimore) ; 102(2): e32493, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36637961

RESUMO

INTRODUCTION: Splenic malignancies are mostly lymphocytic tumors and splenic metastases are rarer.[1] According to reports, the most common source of splenic metastases include melanoma, tumors of the breast, lung, ovary, colon, stomach, and pancreas.[2,3]. PATIENT CONCERNS: This paper reports a 41-year-old male patient who underwent a successful resection of low rectal cancer in our hospital 5 years ago. DIAGNOSIS: Three months ago, computed tomography scan revealed a tumor in the spleen, considered as an isolated metastasis. INTERVENTIONS: The patient underwent splenectomy and postoperative pathological examination confirmed metastatic adenocarcinoma. OUTCOMES: The patient was followed up for 3 months after surgery, there was no abdominal metastasis or recurrence. CONCLUSION: The splenic metastasis from rectal carcinoma 5 years after surgery is rare. If it is a solitary splenic metastasis, splenectomy can effectively improve the prognosis of patients. We review the literature and report this case.


Assuntos
Adenocarcinoma , Melanoma , Neoplasias Retais , Neoplasias Esplênicas , Masculino , Feminino , Humanos , Adulto , Neoplasias Esplênicas/patologia , Adenocarcinoma/patologia , Tomografia Computadorizada por Raios X , Esplenectomia/métodos , Neoplasias Retais/cirurgia
11.
J Dent Sci ; 18(1): 229-236, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36643235

RESUMO

Background/purpose: Splinting mobile teeth is a choice to improve the patient's oral comfort. The purpose of this study was to assess the survival and stability of mobile anterior mandibular teeth after splinting in patients with periodontitis undergoing supportive periodontal therapy (SPT). Materials and methods: Patients with splinted mobile anterior mandibular teeth were assessed retrospectively. Periodontal statuses were recorded at baseline and follow-up visits. Tooth and splint survival as well as splint repairs were recorded. Multilevel Cox regression analyses were performed to evaluate patient- and tooth-related factors that might have influenced the survival rates of splints. Results: Sixty-one patients, collectively having 161 splints, were followed for an average of 5.44 years. On average, probing depth (PD) of splinted teeth decreased from 4.31 mm to 2.93 mm and clinical attachment loss (CAL) decreased from 5.02 mm to 4.58 mm. Alveolar bone was stable in the follow-up period. None of the splinted teeth were extracted, The overall survival rate of the splints was 65.2%. Splints made of composite resin alone were associated with a higher risk of breakage when compared to splints composed of composite resin with mesh grid strips. Conclusion: Splinting showed long-term survival and splinting combined with periodontal supportive treatment is a feasible option to maintain mobile mandibular anterior teeth.

12.
Sci Total Environ ; 863: 160945, 2023 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-36526205

RESUMO

Glacier surface albedo is an important factor affecting glacier ablation, and a positive feedback mechanism has been observed between the surface albedo and mass balance of glaciers. It is important to understand the driving factors and mechanisms of glacier albedo changes (GAC). Based on the MODIS (Moderate resolution imaging spectroradiometer)-derived MOD10A1 and MYD10A1 albedo products, the glacier albedo trends in each MODIS grid cell during each melt season in High-Mountains Asia (HMA) from 2000 to 2020 were calculated. Decreasing glacier albedo trends were found, with a decline rate of 0.25 × 10-2 yr-1; in addition, the GACs exhibited great spatial differences among the 15 subregions. The geographical detector model (GDM) is a new spatial statistical method that can quantitatively reveal the driving forces of climate factors and light-absorbing particles on GAC under single-factor and two-factor interactions. These driving forces can be measured by the corresponding q value. The results showed that on the whole, solid precipitation (snowfall) had the strongest impact on GAC, followed by the glacier surface temperature. The q values of black carbon (BC) and dust were <0.1, but BC or dust had the greatest q value in the 9 subregions. The effects of each factor differed among different elevation zones. The interaction detector indicated that the q value under the influence of two factors was greater than that under a single factor, and the strongest interaction was between snowfall and BC, followed by between snowfall and dust. In 15 subregions, most of the greatest q values in each region corresponded to an interaction with BC or dust. Here, we obtained the main driving factors of GAC in different regions and emphasized the interactions between climatic factors and light-absorbing particles; these results provide references for further studies of glacier mass balance and surface albedo.

13.
Biomaterials ; 292: 121944, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36495801

RESUMO

The microenvironment in solid tumors drives the fate of cancer cells to ferroptosis, yet the underlying mechanism remains incompletely understood. Herein, we report a metal-free polymer photosensitizer (BDPB) as a new type ferroptosis inducer of starved cancer cells. The polymer consists of boron difluoride dipyrromethene dye as the photosensitizing unit and diisopropyl-ethyl amine as the electron-donating unit. Ultrafast spectroscopy and electron spin resonance mechanistically revealed the prolonged charge-separation process in BDPB, enabling complex-I like one-electron transfer effect to produce O2●-. Unexpectedly, the O2●--generating BDPB nanoparticles (NPs) served to deactivate the AMPK-mTOR signaling pathway in normal-state cancer cells to initiate cell repair activity and survive low-dose phototherapy. However, for cancer cells in a starved state, BDPB NPs triggered glutathione peroxidase 4 downregulation, lipid peroxides accumulation, and death to cancer cells, which was identified as ferroptosis but not apoptosis, necroptosis, or autosis. The application of BDPB NPs sheds new light on the design of individualized ferroptosis inducers for combating cancer progression.


Assuntos
Ferroptose , Neoplasias , Humanos , Fármacos Fotossensibilizantes/farmacologia , Polímeros/farmacologia , Neoplasias/tratamento farmacológico , Apoptose , Microambiente Tumoral
14.
Biol Trace Elem Res ; 200(7): 3326-3335, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34546491

RESUMO

Manganese (Mn) is a crucial trace element for poultry nutrition, and its deficiency compromises tibial cartilage development, leading to perosis and a higher incidence of slipped tendon. Tibial dyschondroplasia (TD) is a metabolic cartilage disease characterized by disruption of endochondral bone formation, which is closely related to extracellular matrix (ECM) degradation, in which Mn deficiency plays an important role. Previous studies have confirmed the role of matrix metalloproteinases (MMPs) in the pathogenesis of TD, but whether dysregulated ECM degradation and MMP expression profiles in growth plate are involved in Mn deficiency-induced avian TD has not been fully elucidated yet. Thus, this study was conducted to clarify these issues. Firstly, we successfully established TD model induced by Mn deficiency in broiler chicks. Mn deficiency decreased the number of chondrocytes, contents of proteoglycan, and type II collagen in tibial growth plate, demonstrating the tibial growth plate damage with enhanced ECM degradation. Also, Mn deficiency inhibited the Nrf2 signaling pathway and enhanced the protein levels of NLRP3, active caspase-1, and active IL-1ß in tibial growth plate, indicating the oxidative stress and inflammatory response in Mn deficiency-induced TD. Additionally, upregulated expression levels of MMPs (MMP1, 9, and 13) were observed in tibial growth plate of Mn deficiency group. In summary, these findings suggest that Mn deficiency-enhanced ECM degradation is involved in avian TD, which may be correlated with oxidative stress, inflammatory response, and upregulation of MMPs.


Assuntos
Osteocondrodisplasias , Doenças das Aves Domésticas , Animais , Galinhas , Matriz Extracelular/metabolismo , Lâmina de Crescimento/metabolismo , Manganês/metabolismo , Metaloproteinases da Matriz/metabolismo , Osteocondrodisplasias/induzido quimicamente , Osteocondrodisplasias/metabolismo , Osteocondrodisplasias/patologia , Doenças das Aves Domésticas/metabolismo , Tíbia/metabolismo
15.
Environ Manage ; 2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36471000

RESUMO

Clarifying the behavior motivation of herders participating in grassland governance has important theoretical and practical significance for the construction of ecological civilization and economic development in pastoral areas. Based on the data from 425 households in the Inner Mongolia Autonomous Region, this paper constructs a Double-Hurdle model to explore the impact of informal institutions and environmental emotion (individual emotion, collective emotion, and intergenerational dependence) on herders' grassland governance. The results show that informal institutions promote herders' willingness and payment for participating in grassland governance, and collective emotions hinder herders' participation, showing a "free-ride" mentality. Informal institutions moderate the relationship between collective emotions and grassland governance behavior. Informal institutions also help herders understand the collective sense of responsibility and morality behind grassland protection, thereby reducing the "free-ride" mentality. Therefore, it is necessary to maintain the informal institutions' role as a "glue" in the traditional culture of pastoral areas and to promote the realization of policy goals.

16.
J Org Chem ; 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36472924

RESUMO

A detailed study of iminyl radical cyclizations of O-aryloximes tethered to alkenes is reported. The reactions can be triggered by either microwave irradiation or conventional heating in an oil bath. A variety of radical traps can be employed, enabling C-C, C-N, C-O, C-S, or C-X bond formation and producing a diverse array of functionalized pyrrolines. Substrates containing an allylic sulfide furnish terminal alkenes by a tandem cyclization-thiyl radical ß-elimination pathway. Cyclizations of hydroxylated substrates exhibit moderate diastereoselectivity that in some cases can partially be attributed to intramolecular hydrogen bonding. Computational studies suggested a possible role for thermodynamics in controlling the stereochemistry of cyclizations. The reaction temperature can be lowered from 120 to 100 °C by employing O-(p-tert-butylphenyl)oximes instead of O-phenyloximes as substrates, and these second-generation iminyl radical precursors can be used in a one-pot oxime ether formation-cyclization that is promoted by conventional heating. The functionalized pyrrolines obtained from these reactions can be conveniently transformed in several different ways.

18.
Fitoterapia ; 165: 105404, 2022 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-36572115

RESUMO

Six new alkaloids (1-6) and six known alkaloids (7-12) were obtained from the stems of Sinomenium acutum. Among them, compounds 1-3 and 6 were four N-oxide alkaloids. The structures and absolute configurations of these new alkaloids were elucidated through comprehensive data of 1D and 2D NMR, HRESIMS and ECD spectra. All isolated compounds were evaluated in vitro for their inhibitory activities against nitric oxide (NO) production and inhibitory effects on AChE. Among them, the sinomenine N-oxide (9) was the most potent NO production inhibitor, with an IC50 value of 23.04 µM.

19.
ACS Appl Mater Interfaces ; 14(51): 57311-57320, 2022 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-36512689

RESUMO

Endowed with a soft and conductive feature, hydrogels have been widely used as interface materials in bioelectronics to fulfill mechanical matching and bidirectional exchange between electronic platforms and living samples. Despite their ionic conductivity, the lack of electron mobility has limited their further applications in biosensing, especially in the field of electrochemical sensing. Here, we propose a Ti3C2/sodium alginate (SA) hybrid hydrogel with not only a tissue-like mechanical strength (down to 80 kPa) but also a combined exchange interface for ions and electrons, realizing both mechanical and electrical coupling toward biological tissues. Due to the shared gelation tendency with cations, the Ti3C2 sheets and SA chains can be easily in situ coassembled through a one-step electrogelation method, making the hybrid hydrogel a well-suited interface layer for device functionalization. In addition, the typical two-dimensional (2D) structure and the abundant active terminals of Ti3C2 have endowed the Ti3C2/SA with a massive loading capacity toward catalytic nanoparticles. For example, the Prussian Blue (PB)-loaded Ti3C2/SA hybrid hydrogel exhibited an excellent electrochemical performance (sensitivity: 600 nA µM-1 cm-2; LOD: 12 nM) toward hydrogen peroxide sensing in tissue fluids, illustrating a promising application potentiality of the hybrid hydrogel in biochemical detection at tissue interfaces.


Assuntos
Alginatos , Hidrogéis , Hidrogéis/química , Alginatos/química , Titânio/química , Condutividade Elétrica , Íons
20.
Biomolecules ; 12(12)2022 11 23.
Artigo em Inglês | MEDLINE | ID: mdl-36551164

RESUMO

The rapid spread of COVID-19 has become a major concern for people's lives and health all around the world. COVID-19 patients in various phases and severity require individualized treatment given that different patients may develop different symptoms. We employed machine learning methods to discover biomarkers that may accurately classify COVID-19 in various disease states and severities in this study. The blood gene expression profiles from 50 COVID-19 patients without intensive care, 50 COVID-19 patients with intensive care, 10 non-COVID-19 individuals without intensive care, and 16 non-COVID-19 individuals with intensive care were analyzed. Boruta was first used to remove irrelevant gene features in the expression profiles, and then, the minimum redundancy maximum relevance was applied to sort the remaining features. The generated feature-ranked list was fed into the incremental feature selection method to discover the essential genes and build powerful classifiers. The molecular mechanism of some biomarker genes was addressed using recent studies, and biological functions enriched by essential genes were examined. Our findings imply that genes including UBE2C, PCLAF, CDK1, CCNB1, MND1, APOBEC3G, TRAF3IP3, CD48, and GZMA play key roles in defining the different states and severity of COVID-19. Thus, a new point of reference is provided for understanding the disease's etiology and facilitating a precise therapy.


Assuntos
COVID-19 , Transcriptoma , Humanos , COVID-19/diagnóstico , COVID-19/genética , Aprendizado de Máquina , Biomarcadores
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