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1.
Dent Mater ; 36(9): 1201-1213, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32576408

RESUMO

OBJECTIVE: To evaluate the influence of light emitted from two Polywave®, LED light-curing units (LCU) on in vivo pulp temperature (PT) rise and signs of acute inflammatory response in pulps of human premolar having deep Class V preparations. METHODS: Sixty intact, first premolars from 15 volunteers requiring extraction received infiltrative anesthesia. A sterile thermocouple probe was inserted within the pulp tissue through a minute occlusal pulp exposure in only 45 teeth (n = 9) to continuously monitor PT (°C). A deep buccal Class V preparation was created, and the surface was exposed to light from a commercial Polywave LCU (Bluephase 20i (20i), Ivoclar Vivadent) or from an experimental LCU (Exp) using the exposure modes (EM): 1s/Exp and 2s/Exp, 10s/20i, 20s/20i, and 60s/20i. Peak PT and PT rise values above baseline (ΔT) data were evaluated using a one-way ANOVA followed by Tukey's post-hoc test (α = 5%). Teeth used for histological and immunohistochemical analyses (n = 3) were extracted approximately 2 h after exposure to the LCU. RESULTS: No significant difference in peak PT and ΔT values was noted between 2s/Exp and 20s/20i groups, which both exhibited higher values than 1s/Exp and 10s/20i groups (p < 0.001). Dilated and congested blood vessels were seen after exposure to 1s/Exp, 2s/Exp, or 60s/20i EMs. The expression of IL-1ß and TNF-α tended to be more intense when higher irradiance was delivered. SIGNIFICANCE: Although higher irradiance delivered over a short exposure caused lower PT rise than 5.5 °C, such EMs should be used with caution, as they have more potential to harm the pulp tissue.


Assuntos
Luzes de Cura Dentária , Cura Luminosa de Adesivos Dentários , Dente Pré-Molar , Resinas Compostas , Polpa Dentária , Humanos , Temperatura
3.
RECIIS (Online) ; 13(4): 887-895, out.-dez. 2019. ilus
Artigo em Português | LILACS | ID: biblio-1047673

RESUMO

Este relato objetiva apresentar o processo de desenvolvimento de um material escrito e ilustrado voltado para o dentista atuante em Unidades de Terapia intensiva, bem como os motivos e justificativas que levaram à decisão de sua necessidade. O cirurgião-dentista tem ampliado sua área de atuação e, por isso, necessita de materiais didáticos que forneçam uma base para essa nova forma de pensar a Odontologia, com um caráter integral e multiprofissional. Por não haver contato dos cirurgiões-dentistas com o ambiente hospitalar durante a graduação, salvo raras exceções, o material didático nesta área foi planejado para abranger desde os conhecimentos básicos até situações que envolvem maior conhecimento para a tomada de decisão. A metodologia utilizada foi o levantamento bibliográfico e a montagem de material escrito na forma de manual, além da produção e tratamento de fotografias ilustrativas, obtidas em um Hospital Universitário durante o processo de cuidado odontológico de pacientes críticos.


This report aims to present the process of developing a written and illustrated material for the dentist in intensive care units, as well as the reasons and justifications that led to the decision of their need. The dental surgeon has expanded his area of practice and, therefore, needs didactic materials that provide a basis for this new way of thinking about Dentistry, with an integral and multiprofessional character. Due to the lack of contact between dental surgeons and the hospital environment during graduation, with few exceptions, the teaching material in this area was designed to cover everything from basic knowledge to situations involving greater knowledge for decision making. The methodology used was the bibliographical survey and the assembly of written material in manual form, as well as the production and treatment of illustrative photographs obtained at a University Hospital during the dental care process of critical patients.


Este relato tiene como objetivo presentar el proceso de desarrollo de un material escrito e ilustrado orientado hacia el dentista actuante en Unidades de Terapia intensiva, así como los motivos y justificaciones que llevaron a la decisión de su necesidad. El cirujano-dentista ha ampliado su área de actuación y, por eso, necesita materiales didácticos que proveen una base para esa nueva forma de pensar la Odontología, con un carácter integral y multiprofesional. Por no haber contacto de los cirujanos-dentistas con el ambiente hospitalario durante la graduación, salvo raras excepciones, el material didáctico en esta área fue planeado para abarcar desde los conocimientos básicos hasta situaciones que involucran mayor conocimiento para la toma de decisión. La metodología utilizada fue el levantamiento bibliográfico y el montaje de material escrito en la forma de manual, además de la producción y tratamiento de fotografías ilustrativas, obtenidas en un Hospital Universitario durante el proceso de cuidado odontológico de pacientes críticos.


Assuntos
Humanos , Materiais de Ensino , Desenvolvimento de Pessoal , Comunicação Interdisciplinar , Equipe Hospitalar de Odontologia , Dicionários de Odontologia como Assunto , Assistência Odontológica , Contenção de Riscos Biológicos , Serviços de Saúde Bucal , Odontólogos , Fotografia , Unidades de Terapia Intensiva
4.
Dent Mater ; 33(11): 1266-1273, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28826611

RESUMO

OBJECTIVE: This study evaluated the temperature increase in swine gingival temperature after exposure to light emitted by a Polywave® LED light curing unit (LCU, Bluephase 20i, Ivoclar Vivadent). METHODS: After local Ethics Committee approval (protocol 711/2015), 40 pigs were subjected to general anesthesia and the LCU tip was placed 5mm from the buccal gingival tissue (GT) close to lower lateral incisors. A thermocouple probe (Thermes WFI, Physitemp) was inserted into the gingival sulcus before and immediately after exposure to light. Real-time temperature (°C) was measured after the following exposure modes were applied: High Power (20s-H, 40s-H, and 60s-H) or Turbo mode (5s-T), either with or without the presence of rubber dam (RD) interposed between the LCU tip and GT (n=10). The presence of gingival lesions after the exposures was also evaluated. Peak temperature (°C) and the temperature increase during exposure over that of the pre-exposure baseline value (ΔT) data were analyzed using 2-way ANOVA followed by Bonferroni's post-hoc test (α=5%). A binary logistic regression analysis determined the risk of gingival lesion development. RESULTS: Without RD, no significant difference in ΔT was observed among 20s-H, 40s-H, and 60s-H groups, which showed the highest temperature values, while the 5s-T exposure showed the lowest ΔT, regardless of RD. RD reduced ΔT only for the 20s-H group (p=0.004). Gingival lesions were predominantly observed using 40s-H, with RD, and 60s-H, with and without RD. SIGNIFICANCE: Exposure to a LCU light might be harmful to swine gingiva only when high radiant exposure values are delivered, regardless of the use of RD.


Assuntos
Luzes de Cura Dentária , Gengiva/efeitos da radiação , Temperatura , Animais , Lesões por Radiação/prevenção & controle , Suínos
5.
J Ethnopharmacol ; 191: 29-40, 2016 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-27301616

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Euphorbia umbellata (leitosinha) is used in southern Brazilian folk medicine to treat gastric problems, as well as for its analgesic and anti-inflammatory properties. AIM OF STUDY: To evaluate the anti-ulcer effects of methanolic bark fraction (MF) against in vivo and in vitro assays, as well as an antioxidant, antibacterial and chromatographic study of this fraction. MATERIALS AND METHODS: In vivo anti-ulcer activity was performed using ethanol and indomethacin models with different MF concentrations (50, 100 or 200mg/Kg). The stomachs of the animals were applied to histological evaluation, and the serum to evaluate the ABTS(•+) radical capture. The 200mg/Kg dose was used to analyze the mechanisms involved in antiulcerogenic properties of methanolic fraction. The in vitro activity was performed using several different antioxidant assays, in addition to anti-Helicobacter pylori and anti-urease experiments. The chromatographic study was carried out by LC-MS analysis. RESULTS: Pharmacological investigation of the MF showed an anti-ulcer potential in ethanol and indomethacin in vivo assays. The material presented a high antioxidant activity for several oxidant in vitro systems (DPPH(•), ABTS(•+), O2(•-), HOCl, TauCl and HRP), as well as an ABTS(•+) capture increasing (7.5%) by the treated animals serum (when compared to the negative control). Prostaglandins, nitric oxide/ cyclic guanosine monophosphate pathway and involvement of the protein components of the glutathione complex are some of the mechanisms related with this potential anti-ulcer action. The histological examination of the stomachs of the animals showed that the MF also prevents local action of offensive agents. Chemical analysis using LC-QTOF-MS revealed the presence of ellagic and gallic acid derivatives and flavonols. CONCLUSION: The findings provide scientific basis to the ethnopharmacological purpose of the studied plant and the biological activities of MF of E. umbellata stem bark may be due to the presence of phenolic compounds.


Assuntos
Antiulcerosos/farmacologia , Euphorbia/química , Extratos Vegetais/farmacologia , Polifenóis/farmacologia , Úlcera Gástrica/prevenção & controle , Estômago/efeitos dos fármacos , Animais , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Antiulcerosos/química , Antiulcerosos/isolamento & purificação , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Benzotiazóis/química , Cromatografia Líquida de Alta Pressão , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Ácido Elágico/isolamento & purificação , Ácido Elágico/farmacologia , Etanol , Etnofarmacologia , Feminino , Flavonóis/isolamento & purificação , Flavonóis/farmacologia , Ácido Gálico/isolamento & purificação , Ácido Gálico/farmacologia , Mucosa Gástrica/metabolismo , Helicobacter pylori/efeitos dos fármacos , Indometacina , Metanol/química , Fitoterapia , Casca de Planta/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Plantas Medicinais , Polifenóis/química , Polifenóis/isolamento & purificação , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos , Solventes/química , Espectrometria de Massas por Ionização por Electrospray , Estômago/patologia , Úlcera Gástrica/induzido quimicamente , Úlcera Gástrica/metabolismo , Úlcera Gástrica/patologia , Ácidos Sulfônicos/química
6.
Case Rep Dent ; 2016: 8690593, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26904312

RESUMO

The nasolabial cyst or Klestadt cyst is a relatively uncommon nonodontogenic cyst that develops in the nasal alar region; it has uncertain pathogenesis. This lesion has slow growth and variable dimensions and is characterized clinically by a floating tumefaction in the nasolabial fold area around the bridge of the nose, causing an elevation of the upper lip and relative facial asymmetry. Diagnosis is primarily made clinically; if necessary, this is complemented by imaging. This paper reports the case of a 39-year-old male patient who complained of pain in the right upper premolar region and poor aesthetics due to a firm tumor in the right wing of the nose. Initially, this was thought to be due to an odontogenic abscess; however, the differential diagnosis was that a nasolabial cyst was communicating with the apex of teeth 14 and 15. Surgical treatment was carried out, followed by histopathological examination and concomitant endodontic treatment of the teeth involved.

7.
Int J Paediatr Dent ; 26(2): 125-33, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25967636

RESUMO

BACKGROUND: The study of MMPs' behavior in carious lesions contributes to the understanding of the mechanisms involved in dentin reorganization after restoration. AIM: To compare the abundance and localization of MMPs 2, 8, and 9 in infected dentin before and after restoration. DESIGN: The sample consisted of 23 young permanent molars with active deep carious lesions. Infected carious dentin samples were collected from the same tooth at baseline and 60 days after cavity lining with GIC and composite resin restoration and processed for immunohistochemistry assays. After digital images were obtained, two calibrated operators analyzed the samples according to the immunostaining intensity and the MMPs' localization. Chi-square test was used for statistical analysis. RESULTS: The intensity of immunostaining for MMP-8 was reduced after 60 days (P = 0.02), and no difference was observed for MMP-2 (P = 0.32) and MMP-9 (P = 0.14). The MMPs' distribution was generalized in the intertubular dentin and absent or located in the intratubular dentin, regardless of the period. CONCLUSION: The sealing of infected carious dentin in young permanent molars reduced the expression of MMP-8, which is consistent with the initial remodeling process of the dentin matrix.


Assuntos
Cárie Dentária/metabolismo , Cárie Dentária/patologia , Cárie Dentária/terapia , Dentina/patologia , Cimentos de Ionômeros de Vidro , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 8 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Adolescente , Brasil , Criança , Resinas Compostas , Dentição Permanente , Feminino , Humanos , Técnicas Imunoenzimáticas , Masculino , Dente Molar
8.
J Prosthodont ; 24(7): 562-568, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25556822

RESUMO

PURPOSE: The objective of this article is to describe a method to construct an intraoral acrylic device that permits a reline material to be added to the inner surface of the palatal plate. MATERIALS AND METHODS: Fifteen 60-day-old adult female rats (Rattus Norvegicus Albinus Wistar), weighing 150 to 250 g were used for this study and allocated to three groups (n = 5): G1, animals wearing a heat-polymerized acrylic resin palatal plate (Lucitone 550) for 14 days; G2, animals wearing a heat-polymerized acrylic resin palatal plate (Lucitone 550) relined with Tokuyama Rebase II for 14 days; and G3, animals maintained under the same conditions as the experimental groups, without wearing palatal plates for 14 days. The manipulation of the animals followed the guidelines of the Brazilian College of Animal Experimentation, under the approval of the animal ethics committee of the State University of Ponta Grossa. The palatal plates covered the whole palate, were fixed in the molar region with light-cured resin, and were kept there for 14 days. The animals received a paste diet and water ad libitum. Before and after the trial period, the rats were weighed individually on a precision scale. Statistical analysis was performed using a two-way analysis of variance (α = 0.05) test for comparison of the animals' weight (g) at time 0 and after 14 days of using the palatal plate. RESULTS: No statistical differences were observed regarding the weight of the animals among the experimental groups in the study. CONCLUSIONS: The individual master impressions, the molar teeth coverage, and the method of cementation with nonadhesive composite resin provided good stability for the palatal plate showed in this study, not disturbing the eating habits and nutrition of the animals. This model seems reproducible, offering adequate histopathological evaluation. Differences in tissue morphology exist between the animals that used the palatal plate and the animals that did not use this device. Use of these palatal plates could clarify how prostheses bring changes in the palatal mucosa of users.

9.
PLoS One ; 9(6): e100293, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24963657

RESUMO

AIM: To assess the histopathological changes of rat palatal mucosa exposed to soft reline materials. METHODS: Forty-five adult female Wistar rats with controlled living conditions and fed ad libitum were employed. Palatal appliances of heat-polymerized acrylic resin Lucitone 550 were manufactured and worn by forty animals during 14 days. Five animals did not use the appliances (G1) and were used to control the appliance influence. One experimental group (n = 10) used the appliances without any relining material (G2) to control the material effect. Three experimental groups (n = 10) received the following soft reline materials below appliances: Dentusoft (G3), Dentuflex (G4), and Trusoft (G5). Appliances from half of each experimental group(n = 5) was immersed in water bath at 55°C for 10 min before use. Animals were slaughtered and the palates were fixed in 10% buffered formalin. Hematoxylin and eosin stained sections of 5 µm were analyzed by computerized planimetry. Cellular compartment, keratin and total epithelial thickness, and basement membrane length were measured to histopathological description.Analysis of variance and Tukey post-hoc test were used to data examination(α = 0.05). RESULTS: For heat-treatment groups, G4 showed less elongated ridges at the basal layer interface (p = 0.037) than G2. When comparing the conditions with and without heat-treatment, only G2 showed a significant decrease in the cellular compartment, keratin layer and total epithelium thickness (p<0.05). CONCLUSION: The post-polymerization for Lucitone 550 was an effective method to reduce the changes in the rat palatal mucosa. The soft reline materials tested did not cause significant histopathological changes in the rat palatal mucosa.


Assuntos
Reembasamento de Dentadura/efeitos adversos , Mucosa Bucal/citologia , Mucosa Bucal/efeitos dos fármacos , Animais , Feminino , Temperatura Alta , Polimerização , Ratos , Ratos Wistar , Testes de Toxicidade
10.
J Ethnopharmacol ; 150(1): 263-9, 2013 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-24008110

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Synadenium grantii Hook f. has traditionally been used to treat various neoplastic diseases in southern Brazil. AIM OF STUDY: Evaluation of the antitumoural potential of Synadenium grantii latex against B16F10 melanoma cell line using in vitro and in vivo models, as well as a phytochemical study of the latex. MATERIALS AND METHODS: The in vitro antitumoural activity was performed using MTT and trypan blue assays with different latex concentrations (1.7 µg-7.0 µg/well and 1.22 mg-4.88 mg/well). Flow cytometry was used to determine the progression of the cell cycle. The in vivo activity was performed by subcutaneously injecting melanoma cells in the dorsum of C57BL6 mice, followed by treating the mice with a popular form of use of the latex (garrafada) administered orally. After sacrificing the animals, histological analysis of the organs was performed by hematoxylin-eosin staining. The phytochemical study of the latex was performed by NMR and chromatographic procedures and the extracts and isolated substances were evaluated by IR, 1D and 2D NMR analysis. RESULTS: The Synadenium grantii latex exhibited decreased cell viability of the melanoma line in a concentration and time-dependent manner, and also cell cycle arrest in the S-G2/M phase. The latex caused a 40% reduction in the volume of tumours of the mice with melanomas. Histological examination of the organs of these animals showed no differences between groups. The phytochemical investigation resulted in the isolation and identification of triterpene euphol and the steroid citrostadienol, which were tested against the strain of melanoma. Euphol showed no antitumoural activity, while the steroid citrostadienol showed reduced cytotoxic activity. CONCLUSION: The Synadenium grantii latex presented in vitro and in vivo cytotoxic effects with antitumoural activity against B16F10 melanoma cells.


Assuntos
Antineoplásicos/uso terapêutico , Euphorbiaceae , Látex/uso terapêutico , Melanoma Experimental/tratamento farmacológico , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Látex/química , Látex/farmacologia , Melanoma Experimental/patologia , Camundongos , Camundongos Endogâmicos C57BL , Fitoterapia , Carga Tumoral/efeitos dos fármacos
11.
Pesqui. bras. odontopediatria clín. integr ; 11(01): 65-71, jul. 2011. tab, ilus, graf
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-874387

RESUMO

Objetivo: Comparar a citologia esfoliativa em base líquida em relação à citologia convencional no diagnóstico de carcinomas da região de cabeça e pescoço. Método: Cento e duas lâminas obtidas de 19 indivíduos portadores de neoplasias malignas foram examinadas. As amostras foram divididas em três grupos: grupo 1 - esfregaços de carcinomas da região de cabeça e pescoço obtidos pela citologia em base líquida; grupo 2 (controle) - amostras de citologia em base líquida provenientes de tecido sadio (região anatômica oposta) e grupo 3 - esfregaços de carcinomas da região de cabeça e pescoço obtidos pela citologia esfoliativa convencional. Os esfregaços foram processados em laboratório e avaliados por meio da microscopia de luz. Os dados foram submetidos aos testes do Qui-quadrado (X2), Análise de Variância (ANOVA) e Teste de Tukey-Kramer (P<0,05). Resultados: Houve uma diferença significativa entre os grupos em relação às alterações celulares sugestivas de malignidade (p<0,05). As lâminas preparadas por meio da citologia em base líquida evidenciaram melhores as células neoplásicas. As células epiteliais encontraram-se mais dissociadas e a amostra foi mais representativa. Houve uma redução de 54,6% no número de amostras insatisfatórias e um ganho de sensibilidade de 53% ao se utilizar a citologia em base líquida. Esta técnica permitiu a obtenção de esfregaços mais homogêneos e lâminas de melhor qualidade proporcionando uma redução no número de amostras insatisfatórias e um aumento de sensibilidade do exame. Conclusão: A citologia esfoliativa em base líquida pode ser indicada como um recurso auxiliar no diagnóstico de lesões bucais, principalmente nos casos de neoplasias malignas de natureza epitelial.


Objective: To compare the liquid-based cytology and conventional exfoliative cytology in the diagnosis of head and neck carcinomas. Methods: One hundred and two slides obtained from 19 individual with malignant neoplasias were examined. The samples were divided into 3 groups: group 1 - head and neck carcinoma smears obtained by liquid-based cytology; group 2 (control) - liquid-based cytology samples obtained from healthy tissue (opposed anatomic region), and group 3 - head and neck carcinoma smears obtained by conventional exfoliative cytology. The smears were processed in laboratory and evaluated by light microscopy. Data were subjected to the chi-square (X2), ANOVA and Tukey-Kramer test (p<0.05). Results: There was statistically significant difference (p<0.05) among the groups for the cellular alterations suggestive of malignancy. The slides prepared by liquid-based cytology permitted better visualization of the neoplastic cells. The epithelial cells were more dissociated and the sample was more representative. The number of unsatisfactory samples decreased by 54.6% and the sensitivity increased by 53% when liquid-based cytology was used. This technique permitted obtaining more homogeneous smears and slides with better quality, reducing the number of unsatisfactory samples and increasing the sensitivity of the exam. Conclusion: Liquid-based cytology can be indicated as an auxiliary resource in the diagnosis of oral lesions, especially in the cases of malignant neoplasias of epithelial origin.


Assuntos
Humanos , Masculino , Feminino , Biologia Celular/instrumentação , Carcinoma , Diagnóstico Bucal , Neoplasias de Cabeça e Pescoço , Mucosa Bucal/patologia , Análise de Variância , Distribuição de Qui-Quadrado
12.
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-614374

RESUMO

Objetivo: Comparar a citologia esfoliativa em base líquida em relação à citologia convencional no diagnóstico de carcinomas da região de cabeça e pescoço. Método: Cento e duas lâminas obtidas de 19 indivíduos portadores de neoplasias malignas foram examinadas. As amostras foram divididas em três grupos: grupo 1 - esfregaços de carcinomas da região de cabeça e pescoço obtidos pela citologia em base líquida; grupo 2 (controle) - amostras de citologia em base líquida provenientes de tecido sadio (região anatômica oposta) e grupo 3 - esfregaços de carcinomas da região de cabeça e pescoço obtidos pela citologia esfoliativa convencional. Os esfregaços foram processados em laboratório e avaliados por meio da microscopia de luz. Os dados foram submetidos aos testes do Qui-quadrado (X2), Análise de Variância (ANOVA) e Teste de Tukey-Kramer (P<0,05). Resultados: Houve uma diferença significativa entre os grupos em relação às alterações celulares sugestivas de malignidade (p<0,05). As lâminas preparadas por meio da citologia em base líquida evidenciaram melhores as células neoplásicas. As células epiteliais encontraram-se mais dissociadas e a amostra foi mais representativa. Houve uma redução de 54,6% no número de amostras insatisfatórias e um ganho de sensibilidade de 53% ao se utilizar a citologia em base líquida. Esta técnica permitiu a obtenção de esfregaços mais homogêneos e lâminas de melhor qualidade proporcionando uma redução no número de amostras insatisfatórias e um aumento de sensibilidade do exame. Conclusão: A citologia esfoliativa em base líquida pode ser indicada como um recurso auxiliar no diagnóstico de lesões bucais, principalmente nos casos de neoplasias malignas de natureza epitelial.


Objective: To compare the liquid-based cytology and conventional exfoliative cytology in the diagnosis of head and neck carcinomas. Methods: One hundred and two slides obtained from 19 individual with malignant neoplasias were examined. The samples were divided into 3 groups: group 1 - head and neck carcinoma smears obtained by liquid-based cytology; group 2 (control) - liquid-based cytology samples obtained from healthy tissue (opposed anatomic region), and group 3 - head and neck carcinoma smears obtained by conventional exfoliative cytology. The smears were processed in laboratory and evaluated by light microscopy. Data were subjected to the chi-square (X2), ANOVA and Tukey-Kramer test (p<0.05). Results: There was statistically significant difference (p<0.05) among the groups for the cellular alterations suggestive of malignancy. The slides prepared by liquid-based cytology permitted better visualization of the neoplastic cells. The epithelial cells were more dissociated and the sample was more representative. The number of unsatisfactory samples decreased by 54.6% and the sensitivity increased by 53% when liquid-based cytology was used. This technique permitted obtaining more homogeneous smears and slides with better quality, reducing the number of unsatisfactory samples and increasing the sensitivity of the exam. Conclusion: Liquid-based cytology can be indicated as an auxiliary resource in the diagnosis of oral lesions, especially in the cases of malignant neoplasias of epithelial origin.


Assuntos
Humanos , Masculino , Feminino , Biologia Celular/instrumentação , Carcinoma , Diagnóstico Bucal , Neoplasias de Cabeça e Pescoço , Mucosa Bucal/patologia , Análise de Variância , Distribuição de Qui-Quadrado
13.
Rev. odontol. UNESP (Online) ; 39(1): 33-39, jan.-fev. 2010. tab
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-874770

RESUMO

Objetivo: O objetivo deste estudo foi comparar a eficácia da citologia esfoliativa em base-líquida (CBL) e da citologia esfoliativa convencional (CEC) no diagnóstico da candidose bucal. Material e método: a amostra foi dividida em dois grupos: a) grupo experimental - formado por 27 indivíduos com candidose bucal (submetidos a CBL e CEC) e b) grupo controle - composto por 30 indivíduos sem nenhuma lesão bucal (submetidos a CBL). Além de avaliar a eficácia dos métodos no diagnóstico de candidose bucal, procurou-se comparar a qualidade das imagens microscópicas, a associação da presença de hifas e/ou pseudohifas com o tipo de célula epitelial e a eficácia de aplicação dos dois métodos após o tratamento com antifúngico. Os esfregaços foram processados em laboratório, analisados por microscopia de luz e submetidos à análise estatística por meio do teste do Qui-quadrado (p < 0,05). Resultado: Os resultados mostraram que a CBL foi mais eficaz do que a CEC devido ao maior número de lâminas contendo hifas e/ou pseudohifas, bem como pela quantidade e dispersão mais uniformes das células epiteliais, o que caracterizou a qualidade superior das imagens neste método. Ao se avaliar a associação de hifas e/ou pseudohifas com o tipo de célula epitelial mais encontrado, observou-se que tanto na CBL quanto na CEC as referidas estruturas estavam mais associadas com células das camadas superficial nucleada e intermediária. Conclusão: embora a citologia em base líquida tenha sido tecnicamente superior à citologia esfoliativa convencional na confecção de lâminas com melhor qualidade e favorecendo a detecção de hifas e pseudohifas, a CBL não demonstrou resultados satisfatórios como um método seguro para o diagnóstico da candidose oral.


Objective: The aim of this study was to compare the efficiency of the liquid based cytology (LBC) and conventional exfoliative cytology (CEC) in the diagnosis of oral candidosis. Material and method: The sample was divided in two groups: a) experimental group - composed by 27 individuals with oral candidosis (submitted to LBC and CEC) and b) control group - composed by 30 individuals with no oral lesion (submitted to LBC). Besides, the quality of the cytological smears; the association between hyphae and/or pseudohyphae with the type of epithelial cell; and the efficacy of the two methods after the treatment with antifungal medication were evaluated. The smears were processed in the laboratory, analyzed by light microscopy and submitted to statistical analysis using Chi-square test (P < 0.05). Result: There were more smears exhibiting hyphaes and/or pseudohyphaes in LBC than CEC. Besides, the quantity and the more uniform dispersion of the epithelial cells on the slide were more present. Thus, this method showed the best quality of the cytological smears. When the association between hyphae and/or pseudohyphae with the type of epithelial cell was analyzed, both LBC (experimental and control groups) and CEC demonstrated that these structures were more associated with nucleated superficial and intermediary epithelial cells. Conclusion: Even so LBC has been technically superior than CEC to the preparing of best quality slides and favoring to the detection of hyphae and/or pseudohyphae. Nevertheless, it did not show satisfactory results as a safe method to the diagnosis of oral candidosis.


Assuntos
Humanos , Biologia Celular , Candidíase , Diagnóstico Bucal , Mucosa Bucal , Saúde Bucal
14.
Rev. clín. pesq. odontol. (Impr.) ; 4(3): 175-180, set.-dez. 2008.
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-617356

RESUMO

OBJETIVOS: Revisar a literatura concernente às relações éticas entre cirurgiões-dentistas e pacientes,especialmente nos casos de extrações dentárias em regime de urgência ou emergência, em prontosocorrosodontológicos. Discutir a validade de documentos assinados pelo paciente ou responsável,autorizando ao cirurgião-dentista a extração de dente passível de tratamento conservador. Discutiras autonomias do profissional e do paciente frente a essa circunstância. METODOLOGIA: Analisouse4.973 prontuários de pacientes atendidos no último trimestre de 2001 no Pronto-SocorroOdontológico do Hospital Universitário Cajuru, da PUCPR, Curitiba, PR, Brasil. Avaliou-se asindicações das exodontias, pelo reexame das radiografias, observando-se a possibilidade de tratamentoconservador dos dentes extraídos, bem como a existência do documento de consentimento informadoassinado pelo paciente. RESULTADOS: Foram realizadas 961 extrações dentárias, em 902 pacientes.748 procedimentos (77,84%) tinham indicação radiográfica para extração e 213 (22,16%) não tinham.Somente para esses últimos havia o consentimento informado assinado pelos pacientes.CONCLUSÃO: Considerando os resultados e as condições do presente estudo, é lícito concluirque os pacientes foram adequadamente informados das situações em que os dentes poderiam serconservados, bem como tiveram oportunidade de optar pela cirurgia. Discute-se no presente artigoas implicações éticas e legais desta situação, muitas vezes frustrante e constrangedora para osprofissionais cirurgiões-dentistas.


OBJECTIVES: To review the literature of the ethical relations between dentist and patients,especially in the cases of urgency dental extrations in hospitals and emergency services. Todiscuss the validity of the document signed by the patient or his responsible person, authorizingthe professional to extract a tooth that the possible could be saved by a conservative treatment.To analyse the autonomies of the professional and the patient considering this circumstance.METHODOLOGY: 4,973 files of patients taken care of in the last trimester of 2001 in theEmergency Service of the University Hospital Cajuru, PUCPR, Curitiba, PR, Brazil. Theindications for tooth extractions were re-evaluated bye re-examination of the X-rays images,as well as the existence of the informed consent document signed by the patients. RESULTS:961 dental extrations had been carried through in 902 patients. 748 procedures (77.84%)had radiographic indication for extration and 213 (22.16%) did not have. For these last onesit only had the informed assent signed for the patients. CONCLUSION: Considering theresults and the conditions of the present study, it is possible to conclude that the patientsadequately had been informed of the situations where the teeth could be conserved, as well ashad had chance to opt to the surgery.


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Adulto Jovem , Pessoa de Meia-Idade , Idoso de 80 Anos ou mais , Relações Dentista-Paciente , Ética Odontológica , Autonomia Profissional , Extração Dentária , Brasil , Registros Médicos , Radiografia Dentária , Distribuição por Sexo
15.
Artigo em Inglês | MEDLINE | ID: mdl-17942340

RESUMO

Rhinoliths are calcified masses formed within the nasal cavity because of the solidification of mucus, debris, or foreign objects by gradual accretion of mineral salts. They are mostly asymptomatic and incidentally diagnosed, although some patients may complain of nasal discharge and obstruction. Ulceration of the nasal mucosa, facial swelling, and bone destruction are less frequently observed but bring about difficulties for differential diagnoses with several pathologic entities. This article presents a case of a 54-year-old woman with a rhinolith causing palatal perforation, focusing on the diagnostic process and histopathologic characteristics.


Assuntos
Cálculos/complicações , Corpos Estranhos/complicações , Cavidade Nasal/cirurgia , Doenças Nasais/complicações , Palato/lesões , Cálculos/diagnóstico por imagem , Cálculos/cirurgia , Diagnóstico Diferencial , Feminino , Corpos Estranhos/diagnóstico por imagem , Corpos Estranhos/cirurgia , Humanos , Arcada Edêntula/diagnóstico por imagem , Litíase/complicações , Litíase/diagnóstico por imagem , Litíase/cirurgia , Pessoa de Meia-Idade , Cavidade Nasal/diagnóstico por imagem , Doenças Nasais/diagnóstico por imagem , Doenças Nasais/cirurgia , Palato/diagnóstico por imagem , Radiografia , Distribuição por Sexo
16.
J Oral Maxillofac Surg ; 65(11): 2248-53, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17954321

RESUMO

PURPOSE: This study was conducted to investigate the morphological effects of the absence of estrogen on alveolar wound healing of young female rats after tooth extraction. MATERIALS AND METHODS: A total of 60 4- to 6-week-old female rats underwent bilateral ovariectomy (OVX) or sham operations. Three weeks later, the first mandibular molars were extracted. Subsequently, the animals were killed by cervical dislocation 3, 5, 7, 14, 21, or 28 days after tooth extraction. The mandibles were removed, and serial transversal sections of mesial alveolus of the first mandibular molars were obtained for histometric analysis. RESULTS: OVX sockets showed significant increases in fibroblasts and collagen content 3 and 5 days after the extractions, followed by significant decreases in these parameters in the subsequent periods. In accordance with the decreased collagen content in the latest period of healing, new bone formation was significantly reduced in the OVX animals. CONCLUSION: These findings suggest that the initial molecular changes observed in the absence of estrogen lead to delayed alveolar wound healing.


Assuntos
Processo Alveolar/fisiopatologia , Dente Molar/cirurgia , Ovariectomia , Extração Dentária , Alvéolo Dental/fisiopatologia , Absorciometria de Fóton , Processo Alveolar/patologia , Animais , Densidade Óssea/fisiologia , Colágeno/análise , Estrogênios/fisiologia , Feminino , Fibroblastos/patologia , Mandíbula/patologia , Mandíbula/fisiopatologia , Mandíbula/cirurgia , Osteogênese/fisiologia , Ovário/fisiologia , Ratos , Ratos Wistar , Tíbia/patologia , Fatores de Tempo , Alvéolo Dental/patologia , Cicatrização/fisiologia
17.
Med Oral Patol Oral Cir Bucal ; 12(5): E344-7, 2007 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-17767095

RESUMO

Plasma cell neoplasia is a lymphoid neoplastic proliferation of B cells. This denomination encloses multiple myeloma (MM), solitary bone plasmacytoma and extramedullary plasmacytoma. MM consists of a clonal proliferation of plasma cells based in the bone marrow, with various degrees of differentiation. Neoplastic cells usually produce great amounts of monoclonal light or heavy chains of immunoglobulin that can be detected in serum or urine. The disease is more frequently in men and the average age at diagnosis is about 60 years. The diagnosis is established by blood and urine exams and medullary biopsy. Patients may present renal failure, bone pain, fatigue, recurrent infections and nervous system dysfunction. Oral manifestations may be the first sign of MM, highlighting the importance of the dentist in the early diagnosis of the disease. Treatment involves mainly irradiation and chemotherapy and the prognosis is generally poor. This paper reports a case of a 65 years old black female who had a complaint of a painful mass in the maxilla that prompted a MM diagnosis.


Assuntos
Neoplasias Maxilares/diagnóstico , Mieloma Múltiplo/diagnóstico , Idoso , Feminino , Humanos
18.
JBC j. bras. clin. odontol. integr ; 9(48): 32-37, jan.-mar. 2005. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-455231

RESUMO

Nevo é um termo genérico que se refere a malformações da pele e da mucosa, podendo ser de natureza congênita ou de desenvolvimento. Os nevos surgem nas camadas epiteliais superficiais ou em qualquer uma das variedades de tecido conjuntivo subjacente ao epitélio. Os nevos melanocíticos intrabucais podem ser classificados de acordo com as suas características histológicas em juncionais, compostos ou intramucosos.Existe uma dificuldade no diagnóstico diferencial entre o nevo melanocítico e as lesões com aspectos clínicos similares. Este trabalho tem por objetivo revisar a literatura sobre os nevos melanocíticos e relatar um caso clínico, descrevendo as características clínicas e histopatológicas do nevo melanocítico do tipo juncional e o diagnóstico diferencial com outras lesões bucais, ressaltando que dentre os nevos melanocíticos que prevalecem na cavidade bucal, o nevo melanocítico juncional é considerado incomum


Assuntos
Humanos , Feminino , Adolescente , Mucosa Bucal/lesões , Nevo Pigmentado/diagnóstico , Nevo , Lesões dos Tecidos Moles
19.
JBC j. bras. clin. odontol. integr ; 8(43): 72-76, jan.-fev. 2004. ilus, CD-ROM
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-851611

RESUMO

A candidose é uma infecção fúngica bastante comum que acomete os seres humanos, podendo afetar também a boca. Esta infecção é causada principalmente pela "Candida albicans", porém, hoje já se sabe que outras espécies de "Candida" também são capazes de causar esta enfermidade. Os pacientes mais acometidos são os imunossuprimidos ou imunocomprometidos, sendo que esta doença afeta, principalmente, pessoas em extremos de idade (crianças e idosos). Clinicamente, a candidose pode apresentar-se de maneira bastante variada, como as formas: pseudomembranosa, eritematosa, hiperplásica, associada a próteses, queilite angular e glossite romboidal mediana. Uma vez diagnosticada a infecção, esta deverá ser tratada com o uso de agentes antifúngicos. Estes fármacos são classificados em agentes poliênicos (nistatina e anfotericina B) e derivados azólicos (miconazol, cetoconazol, fluconazol e itraconazol). Além disso, existem novos medicamentos que estão sendo estudados, como é o caso dos derivados do DNA e terapias alternativas (laser). O objetivo deste artigo é revisar as diferentes condutas frente à candidose


Assuntos
Patologia , Usos Terapêuticos , Terapêutica , Virulência , Usos Terapêuticos
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