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Clin Sci (Lond) ; 135(4): 613-627, 2021 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-33491733


The high disability, mortality and morbidity of diabetic ulcers make it urgent to explore effective strategies for diabetic wound repair. TrxR1 plays a vital role in regulating redox homeostasis in various pathologies. In the present study, the effect of berberine (BBR) on diabetic wounds was investigated in streptozotocin (STZ)-induced diabetic rats and a high glucose (HG)-induced cell model, and the mechanism of BBR on TrxR1 was elucidated. BBR treatment remarkably accelerated wound healing and enhanced extracellular matrix (ECM) synthesis and significantly inhibited HG-induced HaCaT cell damage. Further analysis indicated that BBR activated TrxR1, suppressed its downstream JNK signaling, thereby inhibiting oxidative stress and apoptosis, promoted cell proliferation, down-regulated matrix metalloproteinase (MMP) 9 (MMP9) and up-regulated transforming growth factor-ß1 (TGF-ß1) and tissue inhibitors of MMP 1 (TIMP1), resulting in accelerated wound healing. Importantly, the enhancement of BBR on wound repair was further abolished by TrxR1 inhibitor. Moreover, in diabetic wounds induced by a combination of STZ injection and high-fat diet, BBR significantly increased wound closure rate and TrxR1 expression, and this was reversed by TrxR1 inhibitor. These data indicated that topical BBR treatment accelerated diabetic wound healing by activating TrxR1. Targeting TrxR1 may be a novel, effective strategy for restoring redox homeostasis and promoting diabetic wound healing.

Front Pharmacol ; 11: 711, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32581779


Background: Amomi fructus is a famous traditional Chinese medicine (TCM) that can exert beneficial effects during the treatment of gastrointestinal diseases and is used widely in China and other countries in Southeast Asia. However, the nonvolatile active ingredients that are present in the water extractions from A. fructus used to treat gastrointestinal diseases have yet to be elucidated. The goal of this study was to identify the nonvolatile active ingredients of A. fructus. Methods: We used an in situ single-pass intestinal perfusion (SPIP) model to identify the active ingredients of A. fructus that play significant roles in gastrointestinal absorption. In addition, we developed a high-performance liquid chromatography (HPLC) method to identify key fractions in intestinal outflow perfusate. Results: Nineteen components were identified in a water extraction from A. fructus; these exhibited different absorption capabilities in different intestinal segments. Of these, six components were determined by the newly developed HPLC method: catechin, vanillic acid, epicatechin, polydatin, isoquercitrin, and quercitrin. Conclusions: The current study aimed to identify the active ingredients present in water extractions prepared from A. fructus in a single-intestinal perfusate from rats. Our findings provide an experimental basis to explain the pharmacodynamic actions of A. fructus.

Artigo em Inglês | MEDLINE | ID: mdl-32351608


Mirabilishimalaica (Edgew) Heim (MH) is an important Tibetan medicine with demonstrated medicinal efficacy and promising developmental value. A previous study of MH was limited to vague morphological and microscopic descriptions, restricting its clinical application and further development as a medicine. The goal of this study was to comprehensively characterize wild and cultivated products of MH using macroscopic and microscopic identification using HPLC fingerprint. The results revealed that the cultivated and wild MH exhibited differences in macroscopic and microscopic characteristics and chemical components. This analysis can facilitate the establishment of a more comprehensive quality evaluation method for MH. These results provide the basis for clinical applications and the improvement of quality standards of MH as a step towards modernization of Tibetan medicine.