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1.
J Clin Lab Anal ; : e23218, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31968147

RESUMO

INTRODUCTION: Hemolysis is the main cause of unqualified clinical samples. In this study, we established a method for detecting and evaluating hemolysis in whole blood test. We used a mathematical formula for correcting the influence of hemolysis on complete blood cell count (CBC) so as to avoid re-venipuncture and obtain more accurate parameters of red blood cell detection, reduce the burden of patients, and improve the efficiency of diagnosis and treatment. METHODS: Hemolytic samples were selected and then corrected using the new formula. Plasma free hemoglobin (fHB) was used as the criterion to determine the degree of hemolysis; the uncertainty of measurement is acceptable as the limit value of deviation between the measured value and the revised value. Hemolysis simulation analysis in vitro and continuous monitoring of clinical patients were used to verify the correction effect. RESULTS: A total of 83 clinical samples with hemolysis were collected and analyzed; fHB 1.4 g/L was selected as the unacceptable value for clinical hemolysis detection. In hemolytic samples, the red blood cell parameters corrected by formula are significantly different from those uncorrected and had a good consistency with those before hemolysis. CONCLUSION: The results show that the hemolysis phenomenon of CBC has a significant impact on routine blood testing. By using the new formula, the influence of hemolysis on erythrocyte and related parameters can be quickly and easily corrected, thus avoiding venipuncture again for re-examination, reducing diagnostic errors, and saving medical resources.

2.
Front Genet ; 10: 980, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31681420

RESUMO

Seed dormancy and germination are important agronomic traits in wheat (Triticum aestivum L.) because they determine pre-harvest sprouting (PHS) resistance and thus affect grain production. These processes are regulated by Gibberellic Acid-Stimulated Regulator (GASR) genes. In this study, we identified 37 GASR genes in common wheat, which were designated TaGASR1-37. Moreover, we identified 40 pairs of paralogous genes, of which only one had a Ka/Ks value greater than 1, indicating that most TaGASR genes have undergone negative selection. Chromosomal location and duplication analysis revealed 25 pairs of segmentally duplicated genes and seven pairs of tandemly duplicated genes, suggesting that large-scale duplication events may have contributed to the expansion of TaGASR gene family. Microarray analysis of the expression of 18 TaGASR genes indicated that these genes play diverse roles in different biological processes. Using wheat varieties with contrasting seed dormancy phenotypes, we investigated the expression patterns of TaGASR genes and the corresponding seed germination index phenotypes in response to water imbibition, exogenous ABA and GA treatment, and low- and high-temperature treatment. Based on these data, we identified the TaGASR34 gene as potentially associated with seed dormancy and germination. Further, we used a SNP mutation of the TaGASR34 promoter (-16) to develop the CAPS marker GS34-7B, which was then used to validate the association of TaGASR34 with seed dormancy and germination by evaluating two natural populations across environments. Notably, the frequency of the high-dormancy GS34-7Bb allele was significantly lower than that of the low-dormancy GS34-7Ba allele, implying that the favorable GS34-7Bb allele has not previously been used in wheat breeding. These results provide valuable information for further functional analysis of TaGASR genes and present a useful gene and marker combination for future improvement of PHS resistance in wheat.

3.
Theor Appl Genet ; 132(11): 2947-2963, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31324930

RESUMO

KEY MESSAGE: Three major loci for pre-harvest sprouting tolerance (PHST) were mapped on chromosomes 1AL, 3BS, and 6BL, and two CAPS and one dCAPS markers were validated. Sixteen lines with favorable alleles and increased PHST were identified. Pre-harvest sprouting (PHS) significantly affects wheat grain yield and quality. In the present study, the PHS tolerance (PHST) of 192 wheat varieties (lines) was evaluated by assessment of field sprouting, seed germination index, and period of dormancy in different environments. A high-density Illumina iSelect 90K SNP array was used to genotype the panel. A genome-wide association study (GWAS) based on single- and multi-locus mixed linear models was used to detect loci for PHST. The single-locus model identified 23 loci for PHST (P < 0.0001) and explained 6.0-18.9% of the phenotypic variance. Twenty loci were consistent with known quantitative trait loci (QTLs). Three single-nucleotide polymorphism markers closely linked with three major loci (Qphs.ahau-1A, Qphs.ahau-3B, and Qphs.ahau-6B) on chromosomes 1AL, 3BS, and 6BL, respectively, were converted to two cleaved amplified polymorphic sequences (CAPS) and one derived-CAPS markers, and validated in 374 wheat varieties (lines). The CAPS marker EX06323 for Qphs.ahau-6B co-segregated with a novel major QTL underlying PHST in a recombinant inbred line population raised from the cross Jing 411 × Wanxianbaimaizi. Linear regression showed a clear dependence of PHST on the number of favorable alleles. Sixteen varieties showing an elevated degree of PHST were identified and harbored more than 16 favorable alleles. The multi-locus model detected 39 marker-trait associations for PHST (P < 0.0001), of which five may be novel. Six loci common to the two models were identified. The combination of the two GWAS methods contributes to efficient dissection of the complex genetic mechanism of PHST.


Assuntos
Germinação/genética , Triticum/genética , Alelos , Mapeamento Cromossômico , Estudos de Associação Genética , Marcadores Genéticos , Genótipo , Desequilíbrio de Ligação , Modelos Genéticos , Fenótipo , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas , Sementes/fisiologia , Triticum/fisiologia
4.
Inorg Chem ; 57(20): 12452-12455, 2018 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-30246527

RESUMO

The reaction of the amidinatosilicon(I) dimer [LSi:]2 (1; L = PhC(N tBu)2) with FeBr2 in tetrahydrofuran (THF) at ambient temperature afforded the silicon(I)-iron(II) dimer [LSi(FeBr2·THF)]2 (2) after 40 h. Compound 2 can catalyze hydroboration of aliphatic and aromatic ketone compounds with HBpin in the absence of any strong reducing agent. Mechanistic studies show that complex 2 reacts with ketone compounds to form a zwitterionic intermediate in the first step of catalysis. Subsequent reaction with HBpin affords the corresponding boron esters and then regenerates complex 2.

5.
Chemistry ; 24(54): 14329-14334, 2018 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-30102001

RESUMO

The synthesis of a dimeric base-stabilized cobaltosilylene complex and its catalytic reactions are described. Treatment of the amidinato silicon(I) dimer [LSi:]2 (1; L=PhC(NtBu)2 ) with CoBr2 in toluene for 10 days afforded the dimeric amidinato cobaltosilylene [(LSi)µ-{CoBr(LSiBr)}]2 (2), which is speculated to proceed via "LSiCoBr" and "LSiBr" intermediates in the reaction. Compound 2 is paramagnetic, with an effective magnetic moment of 2.8 µB. Its electronic structure was elucidated by single-crystal X-ray crystallography and DFT studies. It was capable of catalyzing C-H bond functionalization, in which a combination of 2, phosphine and MeMgI can regio- and stereoselectively promoted the addition of the C≡C triple bonds in alkynes to the ortho-C-H position in arylpyridines. In addition, compound 2 catalyzed Kumada-type coupling reactions between aryl chlorides and the Grignard reagent 2-mesitylmagnesium bromide.

6.
Ann Clin Lab Sci ; 48(2): 236-241, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29678853

RESUMO

AIMS: The aim of this study is to investigate whether red cell distribution width (RDW) is associated with renal function damage in patients with type 1 diabetes mellitus (T1DM) of children in China. METHODS: We used urine albumin-creatinine ratio to define microalbuminuria (MAU). A total of 170 patients were recruited in the study including 88 patients with MAU and 82 patients without MAU. Clinical and laboratory data of two groups were compared. RESULTS: The present study demonstrated that the RDW values were significantly higher in patients with MAU than those without MAU. Multivariate logistic regression indicated that RDW was an independent risk factor for renal function damage in T1DM. The receiver operating characteristic curves were used to investigate the relationship between MAU and RDW, the area under the curve was 0.75. Using the cut-off point of 12.8, RDW predicts renal function damage in T1DM patients with a sensitivity of 75.8% and a specificity of 58.2%. CONCLUSION: In this study, we suggested that RDW could be used as an effective predictor of diabetic early renal function damage or diabetes-related complications.


Assuntos
Diabetes Mellitus Tipo 1/complicações , Índices de Eritrócitos/fisiologia , Insuficiência Renal , Adolescente , Albuminúria/etiologia , Criança , Pré-Escolar , China , Creatinina/urina , Feminino , Humanos , Masculino , Curva ROC , Insuficiência Renal/sangue , Insuficiência Renal/etiologia , Insuficiência Renal/patologia , Fatores de Risco
7.
Org Lett ; 18(23): 6120-6123, 2016 12 02.
Artigo em Inglês | MEDLINE | ID: mdl-27934365

RESUMO

Palladium-catalyzed α-arylation reactions of ketones with simultaneous ortho alkylation offer 1,2,3-substituted arenes. The reactions of 1,ω-dihaloalkanes also allow facile construction of medicinally important tetralines and benzocycloheptenes.

8.
Sci Rep ; 6: 32143, 2016 10 03.
Artigo em Inglês | MEDLINE | ID: mdl-27694957

RESUMO

Abscisic acid (ABA) is one of the most important phytohormones involved in stress responses in plants. However, knowledge of the effect on ABA distribution and transport of water stress at different sites on the plant is limited. In this study, water stress imposed on peanut leaves or roots by treatment with PEG 6000 is termed "leaf stress" or "root stress", respectively. Immunoenzyme localization technolony was first used to detect ABA distribution in peanut. Under root stress, ABA biosynthesis and distribution level were all more pronounced in root than in leaf. However, ABA transport and the ability to induce stomatal closure were still better in leaf than in root during root stress; However, ABA biosynthesis initially increased in leaf, then rapidly accumulated in the vascular cambium of leaves and induced stomatal closure under leaf stress; ABA produced in root tissues was also transported to leaf tissues to maintain stomatal closure. The vascular system was involved in the coordination and integration of this complex regulatory mechanism for ABA signal accumulation. Water stress subject to root or leaf results in different of ABA biosynthesis and transport ability that trigger stoma close in peanut.


Assuntos
Ácido Abscísico/metabolismo , Arachis/metabolismo , Folhas de Planta/metabolismo , Raízes de Plantas/metabolismo , Arachis/fisiologia , Transporte Biológico , Dioxigenases/metabolismo , Secas , Proteínas de Plantas/metabolismo , Estômatos de Plantas/fisiologia , Estresse Fisiológico
9.
Front Plant Sci ; 7: 1902, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-28066462

RESUMO

Thousand-grain weight (TGW) of wheat (Triticum aestivum L.) contributes significantly to grain yield. In the present study, a candidate gene associated with TGW was identified through specific-locus amplified fragment sequencing (SLAF-seq) of DNA bulks of recombinant inbred lines (RIL) derived from the cross between Jing 411 and Hongmangchun 21. The gene was located on chromosome 7A, designated as TaTGW-7A with a complete genome sequence and an open reading frame (ORF). A single nucleotide polymorphism (SNP) was present in the first exon between two alleles at TaTGW-7A locus, resulting in a Val to Ala substitution, corresponding to a change from higher to lower TGW. Cleaved amplified polymorphic sequence (CAPS) (TGW7A) and InDel (TG9) markers were developed to discriminate the two alleles TaTGW-7Aa and TaTGW-7Ab for higher and lower TGW, respectively. A major QTL co-segregating with TaTGW-7A explained 21.7-27.1% of phenotypic variance for TGW in the RIL population across five environments. The association of TaTGW-7A with TGW was further validated in a natural population and Chinese mini-core collections. Quantitative real-time PCR revealed higher transcript levels of TaTGW-7Aa than those of TaTGW-7Ab during grain development. High frequencies of the superior allele TaTGW-7Aa for higher TGW in Chinese mini-core collections (65.0%) and 501 wheat varieties (86.0%) indicated a strong and positive selection of this allele in wheat breeding. The molecular markers TGW7A and TG9 can be used for improvement of TGW in breeding programs.

10.
Chem Sci ; 7(7): 4067-4072, 2016 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30155050

RESUMO

An efficient and practical synthesis of benzothiazine by K2S initiated sulfur insertion reaction with enaminones via electron catalysis is developed. This protocol provides a new, environment-friendly and simple strategy to construct benzothiazine derivatives via formation of two C-S bonds under transition metal-free, additive-free and oxidant-free conditions. K2S not only provides the sulfur insertion source, but also ignites the reaction through the formation of a trisulfur radical anion and electrons in DMF.

11.
Gene ; 554(2): 241-8, 2015 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-25445293

RESUMO

Many efficient methods have been proposed to advance protein structural class prediction, but there are still some challenges where additional insight or technology is needed for low-similarity sequences. In this work, we schemed out a new prediction method for low-similarity datasets using reduced PSSM and position-based secondary structural features. We evaluated the proposed method with four experiments and compared it with the available competing prediction methods. The results indicate that the proposed method achieved the best performance among the evaluated methods, with overall accuracy 3-5% higher than the existing best-performing method. This paper also found that the reduced alphabets with size 13 simplify PSSM structures efficiently while reserving its maximal information. This understanding can be used to design more powerful prediction methods for protein structural class.


Assuntos
Biologia Computacional/métodos , Proteínas/química , Proteínas/classificação , Algoritmos , Bases de Dados de Proteínas , Matrizes de Pontuação de Posição Específica , Estrutura Secundária de Proteína , Homologia de Sequência de Aminoácidos
12.
Chem Commun (Camb) ; 50(85): 12892-5, 2014 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-25212535

RESUMO

Mn(III)-mediated reactions of 2-isocyanobiaryls with 1,3-dicarbonyl compounds were described for the construction of 6-alkylated and 6-monofluoro-alkylated phenanthridines in moderate to good yields. The reaction involves formation of two new C-C bonds and one C-C bond cleavage.


Assuntos
Manganês/química , Fenantridinas/síntese química , Alquilação , Catálise , Halogenação , Fenantridinas/química
13.
Chem Commun (Camb) ; 50(49): 6439-42, 2014 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-24699898

RESUMO

An efficient method for the construction of 6-alkyl phenanthridines by tert-butyl peroxybenzoate (TBPB)-mediated 2-isocyanobiaryl insertion with 1,4-dioxane was established. Two new C-C bonds were formed in this reaction via a sequential C(sp(3))-H/C(sp(2))-H bond functionalization under metal-free conditions.

14.
Guang Pu Xue Yu Guang Pu Fen Xi ; 30(1): 78-82, 2010 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-20302086

RESUMO

Ganoderma lucidum, ganoderma atrum, ganderma tsugae Murr. and ganoderma lipsiense can be discriminated and identified by using multi-steps infrared macro-fingerprint method. The 1D-1R spectra, based on the peaks intensity at 1153 and 1078 cm(-1), which are the fingerprint characteristic peaks of glucoside compounds, show that the content of glucoside compounds of them was in the order of: ganoderma lucidum>ganoderma atrum>ganderma tsugae Murr. >ganoderma lipsiense. Generally, the second derivative IR spectra can clearly enhance the spectra resolution. In the range of 1600-1720 cm(-1), the position and sharpness of characteristics peaks were very different, and it's proved that amino acid peptide compounds of them were different. In the 2D-IR spectra, four of them have the same autopeak at 1100 cm(-1), which is the autopeaks of glucoside, but the number of autopeaks of ganoderma lucidum was 4 and its strongest autopeak was 1040 cm(-1), while 5 autopeaks, 4 autopeaks and 5 autopeaks were for ganoderma atrum, ganderma tsugae Murr. and ganoderma lipsiense respectively, and their strongest autopeaks were 1040, 1139, 1140 and 1134 cm(-1) respectively. The multi-steps infrared maro-fingerprint identification testified that the contents of glucoside compounds and amino acid peptide compounds in these four kinds of ganoderma are different. It's proved that multi-steps infrared maro-fingerprint method can be used to analyze and distinguish ganoderma lucidum, ganoderma atrum, ganderma tsugae Murr. and ganoderma lipsiense.


Assuntos
Ganoderma/classificação , Espectrofotometria Infravermelho , Aminoácidos/química , Ganoderma/química , Glucosídeos/química , Especificidade da Espécie
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