Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 26
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Sci Total Environ ; 792: 148396, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34465046

RESUMO

Droughts represent one of the most severe abiotic stress factors that could result in great crop yield loss. Numerous vegetation indices have been proposed for monitoring the vegetation condition under stress and assessing drought impacts on yield loss. However, the understanding and comparison between traditional vegetation indices (VIs) and the newly emerging satellite Sun-Induced Chlorophyll Fluorescence (SIF) for monitoring vegetation condition is still limited especially under drought stress and at multiple spatial scales. In this study, the potential of satellite observation SIF for monitoring corn response to drought was investigated based on the 2012 drought in the US Corn Belt. The standardized precipitation evapotranspiration index (SPEI) was used here to quantify drought. We found that all SPEI were above -1, except for July (-1.27), August (-1.39) and September (-1.14) in 2012, indicating the severity of this drought. We examined the relationship between satellite measurements of SIF, SIFyield, VIs (e.g., NDVI and EVI) and SPEI. Results indicated that SIFyield was sensitive to drought and SIF captured the stress more accurately both at the regional and state scales for the US Corn Belt. Quantitatively, SIFyield had a high correlation with SPEI (r = 0.987, p < 0.05) over the entire Corn Belt, and it indicated losses in response to drought approximately one month earlier than SIF/NDVI/EVI. Furthermore, our results demonstrated that SIF could be trusted as an effective indicator to study the relationship between GPP (R2 ≥ 0.8664, p < 0.01) under drought conditions across the Corn Belt. This study highlighted the advantage of using satellite SIF observations to monitor the drought stress on crop growth especially GPP at regional scale.


Assuntos
Clorofila , Secas , Fluorescência , Estações do Ano , Zea mays
2.
Am J Physiol Lung Cell Mol Physiol ; 319(4): L742-L751, 2020 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-32783621

RESUMO

Prenatal smoke exposure is a risk factor for impaired lung development in children. Recent studies have indicated that amphiregulin (AREG), which is a ligand of the epidermal growth factor receptor (EGFR), has a regulatory role in airway epithelial cell differentiation. In this study, we investigated the effect of prenatal smoke exposure on lung epithelial cell differentiation and linked this with AREG-EGFR signaling in 1-day-old mouse offspring. Bronchial and alveolar epithelial cell differentiations were assessed by immunohistochemistry. Areg, epidermal growth factor (Egf), and mRNA expressions of specific markers for bronchial and alveolar epithelial cells were assessed by RT-qPCR. The results in neonatal lungs were validated in an AREG-treated three-dimensional mouse lung organoid model. We found that prenatal smoke exposure reduced the number of ciliated cells and the expression of the cilia-related transcription factor Foxj1, whereas it resulted in higher expression of mucus-related transcription factors Spdef and Foxm1 in the lung. Moreover, prenatally smoke-exposed offspring had higher numbers of alveolar epithelial type II cells (AECII) and lower expression of the AECI-related Pdpn and Gramd2 markers. This was accompanied by higher expression of Areg and lower expression of Egf in prenatally smoke-exposed offspring. In bronchial organoids, AREG treatment resulted in fewer ciliated cells and more basal cells when compared with non-treated bronchiolar organoids. In alveolar organoids, AREG treatment led to more AECII cells than non-treated AECII cells. Taken together, the observed impaired bronchial and alveolar cell development in prenatally smoke-exposed neonatal offspring may be induced by increased AREG-EGFR signaling.


Assuntos
Anfirregulina/metabolismo , Anfirregulina/farmacologia , Células Epiteliais/efeitos dos fármacos , Receptores ErbB/metabolismo , Fumaça/efeitos adversos , Animais , Animais Recém-Nascidos , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/fisiologia , Fator de Crescimento Epidérmico/metabolismo , Células Epiteliais/metabolismo , Camundongos , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Tabaco/efeitos adversos
3.
Mol Omics ; 15(6): 459-469, 2019 12 02.
Artigo em Inglês | MEDLINE | ID: mdl-31755891

RESUMO

Accumulating evidence has highlighted the important roles of long non-coding RNAs (lncRNAs) acting as competing endogenous RNAs (ceRNAs) in Alzheimer's disease (AD). In this study, we constructed an AD-derived lncRNA-associated ceRNA network (LncACeNET) based on the ceRNA hypothesis and co-expressed correlation analysis of RNAs (miRNAs, mRNAs and lncRNAs) from AD patients. Based on this network, we preliminarily identified new potential AD biomarkers including hsa-miR-155-5p, CERS6-AS1, and CTB-89H12.4. The functional enrichment analysis demonstrated that these inferred biomarkers were significantly correlated with AD-related biological processes such as neuron projection development and neuron projection morphogenesis. Notably, lncRNA CTB-89H12.4 is significantly associated with "calcium ion-regulated exocytosis of neurotransmitter", "chemical synaptic transmission", "presynaptic membrane assembly", "receptor localization to synapse", and "learning". This indicates the important role of CTB-89H12.4 as a promising target for AD therapy. Subsequently, we used the computational pipeline DTINet and discovered 19 lines of probable therapeutic relationships between FDA-approved drugs and CTB-89H12.4, which offered a new avenue to repurpose existing FDA-approved drugs for AD indication. Our study provides a new landscape for LncACeNET in AD, and will benefit mechanism study and new drug development for AD.


Assuntos
Doença de Alzheimer/genética , Biologia Computacional/métodos , Regulação da Expressão Gênica , Redes Reguladoras de Genes , MicroRNAs/genética , RNA Longo não Codificante/genética , RNA Mensageiro/genética , Algoritmos , Descoberta de Drogas/métodos , Humanos , Anotação de Sequência Molecular , Software , Navegador
4.
Anal Bioanal Chem ; 411(30): 8033-8042, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31781813

RESUMO

Lateral flow immunoassays (LFIAs) are popular because they are rapid, convenient, stable, low cost, and easy to read. However, conventional LFIAs based on gold nanoparticles lack sensitivity, which hinders their widespread use. Here, we prepared durian-like gold nanoparticles (GNDs) and labeled them with staphylococcal protein A to detect brucella antibody. Then, the analytical performances of GNDs and gold nanospheres (GNSs) with the same diameter were compared. It was found that the sensitivity of GNDs was five to ten times higher than that of GNSs. The nonspherical morphologies of the nanoparticles greatly increased the sensitivity of the LFIA. On the basis of GNDs and GNSs, we developed an ultrasensitive dual-color brucellosis LFIA. GNSs labeled with streptavidin were used to demonstrate the control line. This dual-color LFIA had a diagnostic sensitivity and specificity of 100%. Human standard Brucella-positive serum (containing brucella antibody at 4000 IU/mL) could be detected in this system even for a dilution factor of 10-5. The detection limit was 0.04 IU/mL. This is two orders of magnitude better than conventional LFIA strips (detection limit 4 IU/mL). This dual-color LFIA contains all components of a conventional LFIA with no additional processing steps or reagents. It can detect antibodies in serum, plasma, and even whole blood without sample pretreatment or blood filtration pads. Both types of nanoparticles were synthesized in a simple and low-cost manner. This suggests that it will have utility for the early diagnosis of brucellosis and other diseases. Graphical abstract.


Assuntos
Brucelose/diagnóstico , Ouro/química , Imunoensaio/métodos , Nanopartículas Metálicas/química , Testes Sorológicos/métodos , Cor , Humanos , Limite de Detecção
5.
Int J Endocrinol ; 2019: 6785289, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31263496

RESUMO

Testosterone plays an important prenatal role in male testis development. Bisphenol A (BPA) exposure during pregnancy affects testosterone levels and germ cell apoptosis of male pups, but little information is available for the mechanism. The aim of the present study was to investigate the mechanism by which BPA alters testosterone levels and germ cell apoptosis. Pregnant female C57BL/6J mice, throughout gestation, had access to drinking water containing BPA at 5 and 50 µg/mL. Male pups were euthanized on postnatal days (PNDs) 1, 14, and 35. Relative to control, BPA exposure at 5 and 50 µg/ml decreased testosterone level, as measured by chemiluminescent immunoassay, on PND14. Real-time PCR indicated mRNA levels for steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (CYP11A1), and 3-ß-hydroxysteroid dehydrogenase/△-5-4 isomerase (3ß-HSD) were significantly lower in the BPA pups compared to control. Additionally, BPA increased the percentage of TUNEL-positive seminiferous tubules, decreased the mRNA level of Bcl-2, and increased Bax expression, indicative of increased apoptosis. These results suggest that BPA exposure in utero decreases the testosterone concentration by decreasing steroidogenic enzymes (StAR, CYP11A1, and 3ß-HSD). Furthermore, BPA exposure increases the apoptosis of germ cells, which is associated with proapoptotic changes in the levels of Bcl-2 and Bax.

6.
World J Microbiol Biotechnol ; 35(2): 31, 2019 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-30701329

RESUMO

In order to establish a rapid detection method for Mycoplasma ovipneumoniae, this study used the loop-mediated isothermal amplification (LAMP) technique to carry out nucleic acid amplification and chromatographic visualization via a lateral flow dipstick (LFD) assay. The M. ovipneumoniae elongation factor TU gene (EF-TU) was detected using a set of specific primers designed for the EF-TU gene, and the EF-TU FIP was detected by biotin labeling, which was used in the LAMP amplification reaction. The digoxin-labeled probe specifically hybridized with LAMP products, which were visually detected by LFD. Here, we established the M. ovipneumoniae LAMP-LFD rapid detection method and tested the specificity, sensitivity, and clinical application of this method. Results showed that the optimized LAMP performed at 60 °C for 60 min, and LFD can specifically and visually detect M. ovipneumoniae with a minimum detectable concentration at 1.0 × 102 CFU/mL. The sensitivity of LAMP-LFD was 1000 times that of the conventional PCR detection methods, and the clinical lung tissue detection rate was 86% of 50 suspected sheep infected with M. ovipneumoniae. In conclusion, LAMP-LFD was established in this study to detect M. ovipneumoniae, a method that was highly specific, sensitive, and easy to operate, and provides a new method for the prevention and diagnosis of M. ovipneumoniae infection.


Assuntos
Mycoplasma ovipneumoniae/isolamento & purificação , Técnicas de Amplificação de Ácido Nucleico/métodos , Pneumonia por Mycoplasma/veterinária , Doenças dos Ovinos/microbiologia , Animais , Proteínas de Bactérias/genética , Primers do DNA/genética , Humanos , Mycoplasma ovipneumoniae/classificação , Mycoplasma ovipneumoniae/genética , Pneumonia por Mycoplasma/diagnóstico , Pneumonia por Mycoplasma/microbiologia , Sensibilidade e Especificidade , Ovinos , Doenças dos Ovinos/diagnóstico
7.
Chemosphere ; 220: 1118-1125, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33395799

RESUMO

In this work, microwave-assisted hydrothermal process is applied to the PCDD/F degradation of municipal solid waste incineration (MSWI) fly ash. The effects of water-washing pretreatment and the Na2HPO4 reagent on the degradation efficiency of PCDD/Fs are investigated. The PCDD/F content in MSWI fly ash is detected by high-resolution gas chromatography-mass spectrometry (HRGC/MS). The experimental results show that the efficiency of total PCDD/F degradation increases from 60.6% to 83.3% after water-washing pretreatment, with 5 wt % Na2HPO4 added for 2 h of microwave heating at 220 °C. With more Na2HPO4 (10 wt %), the degradation efficiency of PCDD/Fs reaches 91.8%, and remarkably, the WHO-TEQ is as low as 0.255 ng g-1. Analysis of the degradation pathway of PCDD/Fs indicates that a chlorination reaction happens during the microwave-assisted hydrothermal process, as do dechlorination and destruction reactions. Water-washing effectively weakens the chlorination reaction for the decrease of chlorine in fly ash, which contributes to PCDD/F degradation. The reagent Na2HPO4 has a greater effect on the dechlorination of high-chlorinated congeners. Furthermore, the results indicate that the removal efficiency of PCDDs is higher than that of PCDFs under microwave conditions. Several linear correlations between indicative congener content and I/WHO-TEQ values are summarized. Overall, microwave-assisted hydrothermal process is a promising disposal method and should receive further study for large-scale application.

8.
J Nanosci Nanotechnol ; 18(12): 8105-8110, 2018 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-30189926

RESUMO

We herein describe a solvothermal method for preparing superparamagnetic Fe3O4 magnetic colloidal nanocrystal clusters with an average diameter of 195 nm. The Fe3O4 magnetic colloidal nanocrystal clusters were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and other methods. The Fe3O4 magnetic colloidal nanocrystal clusters were used to extract genomic DNA from fresh blood, Gram-negative bacteria, Gram-positive bacteria and frozen blood. Results showed that the genomic DNA integrity, purity and yield extracted via this universal method were satisfactory. This method is rapid, simple and does not use protease K or toxic compounds. This method lays the foundation for future research and nucleic acid extraction techniques.


Assuntos
DNA , Nanopartículas , DNA/genética , DNA/isolamento & purificação , Genômica , Nanopartículas Magnéticas de Óxido de Ferro , Fenômenos Magnéticos
9.
Sci Total Environ ; 616-617: 988-995, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29096958

RESUMO

Chronic exposure to heavy metals could affect cell-mediated immunity. The aim of this study was to explore the status of memory T cell development in preschool children from an e-waste recycling area. Blood lead (Pb) levels, peripheral T cell subpopulations, and serum levels of cytokines (IL-2/IL-7/IL-15), relevant to generation and homeostasis of memory T cells were evaluated in preschool children from Guiyu (e-waste-exposed group) and Haojiang (reference group). The correlations between blood Pb levels and percentages of memory T cell subpopulations were also evaluated. Guiyu children had higher blood Pb levels and increased percentages of CD4+ central memory T cells and CD8+ central memory T cells than in the Haojiang group. Moreover, blood Pb levels were positively associated with the percentages of CD4+ central memory T cells. In contrast, Pb exposure contributed marginally in the change of percentages of CD8+ central memory T cells in children. There was no significant difference in the serum cytokine levels between the e-waste-exposed and reference children. Taken together, preschool children from an e-waste recycling area suffer from relatively higher levels of Pb exposure, which might facilitate the development of CD4+ central memory T cells in these children.


Assuntos
Resíduo Eletrônico/efeitos adversos , Exposição Ambiental/estatística & dados numéricos , Poluentes Ambientais/sangue , Chumbo/sangue , Linfócitos T/fisiologia , Pré-Escolar , Resíduo Eletrônico/estatística & dados numéricos , Feminino , Humanos , Interleucina-15/sangue , Interleucina-2/sangue , Interleucina-7/sangue , Masculino , Reciclagem
10.
Ecotoxicol Environ Saf ; 145: 615-622, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28806563

RESUMO

Heavy metal lead (Pb) and cadmium (Cd) are widespread environmental contaminants and exert detrimental effects on the immune system. We evaluated the association between Pb/Cd exposures and innate immune cells in children from an electronic waste (e-waste) recycling area. A total number of 294 preschool children were recruited, including 153 children from Guiyu (e-waste exposed group), and 141 from Haojiang (reference group). Pb and Cd levels in peripheral blood were measured by graphite furnace atomic absorption spectrophotometer, NK cell percentages were detected by flow cytometer, and other innate immune cells including monocytes, eosinophils, neutrophils and basophils were immediately measured by automated hematology analyzer. Results showed children in Guiyu had significantly higher Pb and Cd levels than in reference group. Absolute counts of monocytes, eosinophils, neutrophils and basophils, as well as percentages of eosinophils and neutrophils were significantly higher in the Guiyu group. In contrast, NK cell percentages were significantly lower in Guiyu group. Pb elicited significant escalation in counts of monocytes, eosinophils and basophils, as well as percentages of monocytes, but decline in percentages of neutrophils in different quintiles with respect to the first quintile of Pb concentrations. Cd induced significant increase in counts and percentages of neutrophils in the highest quintile compared with the first quintile of Cd concentrations. We concluded alteration of the number and percentage of innate immune cells are linked to higher levels of Pb and Cd, which indicates Pb and Cd exposures might affect the innate and adaptive immune response in Guiyu children.


Assuntos
Poluentes Atmosféricos/toxicidade , Resíduo Eletrônico , Imunidade Inata/efeitos dos fármacos , Leucócitos/efeitos dos fármacos , Metais Pesados/toxicidade , Reciclagem , Poluentes Atmosféricos/sangue , Cádmio/sangue , Cádmio/toxicidade , Pré-Escolar , Exposição Ambiental/análise , Feminino , Humanos , Chumbo/sangue , Chumbo/toxicidade , Contagem de Leucócitos , Leucócitos/imunologia , Masculino , Metais Pesados/sangue , Espectrofotometria Atômica
11.
Int J Mol Med ; 39(2): 423-429, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28035406

RESUMO

Autophagy plays an important role in the progression of spinal cord injury (SCI). In this study, we aimed to examine the effects and potential mechanisms of action of BMP and activin membrane-bound inhibitor (BAMBI) in the progression of SCI. A rat model of SCI was established and the rats were injected with pLentiH1-BAMBI shRNA and pAd-BAMBI in the gray and white matter of the spinal cord at T8. After 14 days, motor function evaluation was measured according to the Basso Beattie Bresnahan (BBB) method and the number of motor neuron cell accounts in the anterior horns was measured by Nissl staining. The protein expression of levels light chain 3B (LC3B), Beclin 1, Bim and p62 were measured by western blot analysis. The concentrations of interleukin (IL)-1ß, IL-6 and IL-10 were measured by ELISA. The results revealed that BAMBI expression was significantly decreased in the rats with SCI. The BBB scores and the number of motor neuron cell accounts in the anterior horns were significantly decreased in the pLentiH1-BAMBI shRNA injection group, and were increased in the pAd-BAMBI injection group, suggesting a neuroprotective effect of BAMBI in SCI. Moreover, the increased expression levels of LC3B and Beclin 1, and the decreased expression of Bim and p62 indicated that autophagy was significantly induced in the pAd-BAMBI injection group. Moreover, the overexpression of BAMBI also decreased the expression of transforming growth factor-ß (TGF-ß) and mammalian target of rapamycin (mTOR), and decreased the concentrations of IL-1ß, IL-6 and IL-10. These results indicate that BAMBI plays a neuroprotective role by decreasing inflammation and activating autophagy in rats with SCI.


Assuntos
Autofagia , Inflamação/genética , Proteínas de Membrana/genética , Traumatismos da Medula Espinal/etiologia , Traumatismos da Medula Espinal/metabolismo , Animais , Modelos Animais de Doenças , Expressão Gênica , Técnicas de Silenciamento de Genes , Inativação Gênica , Inflamação/metabolismo , Inflamação/patologia , Locomoção/genética , Proteínas de Membrana/metabolismo , Interferência de RNA , RNA Interferente Pequeno/genética , Ratos , Transdução de Sinais , Traumatismos da Medula Espinal/patologia , Serina-Treonina Quinases TOR/metabolismo
12.
Ann Glob Health ; 82(1): 119-31, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27325070

RESUMO

Evidence has accumulated that exposure to widespread environmental toxicants, such as heavy metals, persistent organic pollutants, and tobacco smoke adversely affect fetal development and organ maturation, even after birth. The developing immune and respiratory systems are more sensitive to environmental toxicants due to their long-term physical development, starting from the early embryonic stage and persisting into early postnatal life, which requires complex signaling pathways that control proliferation and differentiation of highly heterogeneous cell types. In this review, we summarize the effect of early-life exposure to several widespread environmental toxicants on immune and lung development before and after birth, including the effects on immune cell counts, baseline characteristics of cell-mediated and humoral immunity, and alteration of lung structure and function in offspring. We also review evidence supporting the association between early-life exposure to environmental toxicants and risk for immune-related diseases and lung dysfunction in offspring in later life.


Assuntos
Exposição Ambiental/efeitos adversos , Desenvolvimento Fetal/fisiologia , Substâncias Perigosas/toxicidade , Sistema Imunitário/efeitos dos fármacos , Pulmão/embriologia , Pulmão/crescimento & desenvolvimento , Sistema Respiratório/efeitos dos fármacos , Meio Ambiente , Desenvolvimento Fetal/efeitos dos fármacos , Humanos , Hipersensibilidade , Sistema Imunitário/crescimento & desenvolvimento , Pulmão/efeitos dos fármacos , Sistema Respiratório/crescimento & desenvolvimento , Doenças Respiratórias/induzido quimicamente , Doenças Respiratórias/fisiopatologia
13.
Environ Sci Pollut Res Int ; 23(11): 10604-10614, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27040534

RESUMO

The peak occurrence of Mycoplasma pneumoniae (M. pneumoniae) infections in childhood and haze episodes is concurrent. Together, the prevalence of macrolide-resistant M. pneumoniae varies among countries might also be related to the concentration of ambient fine particulate mass (aerodynamic diameter ≤2.5 µm, PM2.5). Numerous cohort studies have identified consistent associations between ambient PM2.5 and cardiorespiratory morbidity and mortality. PM2.5 is a carrier of the heavy metals. The relationship between PM2.5-associated metals and M. pneumoniae infections in childhood has been increasingly drawing public attention. First, we reviewed original articles and review papers in Pubmed and Web of Science regarding M. pneumoniae and PM2.5-associated metal and analyzed the structural basis of PM2.5-associated metal interaction with M. pneumoniae. Then, the possible mechanisms of action between them were conjectured. Mechanisms of oxidative stress induction and modulation of the host immune system and inflammatory responses via Toll-like receptors (TLRs) and/or the nuclear factor-kappa B (NF-κB) pathway are postulated to be the result of PM2.5-associated metal complex interaction with M. pneumoniae. In addition, a heavy metal effect on M. pneumoniae-expressed community-acquired respiratory distress syndrome (CARDS) toxin, and activation of the aryl hydrocarbon receptor (AhR) and TLRs to induce the differentiation of T helper (Th) cells are also regarded as important reasons for the influence of the heavy metals on the severity of M. pneumoniae pneumonia and the initial onset and exacerbation of M. pneumoniae associated asthma. PM2.5-associated metals via complex mechanisms can exert a great impact on the host through interaction with M. pneumoniae.


Assuntos
Poluentes Atmosféricos , Metais , Mycoplasma pneumoniae , Material Particulado , Pneumonia por Mycoplasma , Poluentes Atmosféricos/imunologia , Poluentes Atmosféricos/toxicidade , Humanos , Metais/imunologia , Metais/toxicidade , Mycoplasma pneumoniae/química , Mycoplasma pneumoniae/imunologia , Mycoplasma pneumoniae/patogenicidade , Material Particulado/imunologia , Material Particulado/toxicidade , Pneumonia por Mycoplasma/induzido quimicamente , Pneumonia por Mycoplasma/imunologia , Pneumonia por Mycoplasma/microbiologia , Pneumonia por Mycoplasma/fisiopatologia
14.
Environ Pollut ; 213: 143-150, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26895538

RESUMO

Lead (Pb) has been proved to exert immunotoxicity to influence immune homeostasis in humans. To monitor the internal Pb level and evaluate its effect on natural killer (NK) cells and cytokine/chemokine concentrations, we recruited 285 preschool children from Guiyu, one of the largest electronic waste (e-waste) destinations and recycling areas in the world, and known to have high concentrations of Pb in the air, soil, water, sediment and plants. A total of 126 preschool children were selected from Haojiang as a reference group. Results showed that children in Guiyu, the exposed area, had higher blood Pb levels and lower percentages of NK cells than children from the reference area. A significantly negative association was found between the percentage of NK cells and increasing Pb levels. Moreover, children in Guiyu area had higher platelet counts and IL-1ß concentrations, and lower levels of IL-2, IL-27, MIP-1α and MIP-1ß were observed in the exposed children. These changes might not be conducive to the development and differentiation of NK cells. Taken together, the elevated Pb levels result in the lower percentages of NK cells, but also alter the levels of platelets, IL-1ß and IL-27, which might be unconducive to the activity and function of NK cells.


Assuntos
Resíduo Eletrônico/efeitos adversos , Exposição Ambiental/análise , Poluentes Ambientais/efeitos adversos , Células Matadoras Naturais/efeitos dos fármacos , Chumbo/efeitos adversos , Chumbo/sangue , Poluentes Atmosféricos/efeitos adversos , Pré-Escolar , China , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos , Feminino , Humanos , Masculino , Reciclagem , Poluentes do Solo/efeitos adversos , Poluentes Químicos da Água/efeitos adversos
15.
Sci Rep ; 6: 21004, 2016 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-26867681

RESUMO

Plastic production releases phthalate esters (PAEs), which can alter the expression of metallothioneins (MTs), fatty acid transport protein 1 (FATP1) and heart fatty acid binding protein (HFABP). A total of 187 mother-infant pairs were recruited, 127 from Chenghai (high exposed group) and 60 from Haojiang (low exposed group), to investigate the association between neonatal PAE exposure and mRNA expression of placental MTs, FATP1 and HFABP. Umbilical cord blood and placenta samples were collected for measuring five PAE concentrations and detecting mRNA levels of MTs, FATP1 and HFABP. Butylbenzyl phthalate (BBP), di(2-ethylhexyl) phthalate (DEHP), di-n-octyl phthalate (DNOP) were significantly higher in the high exposed group compared to the low exposed group. FATP1 and HFABP mRNA in the high exposed group were higher than that in the low exposed group while MT-1A was contrary. Both dimethyl phthalate (DMP) and DEHP were correlated with higher MT and MT-2A expression, while diethyl phthalate (DEP) was also positively correlated with MT-1A and FATP1 expression in female infants. DEHP exposure was negatively correlated with birth weight and gestational age in male infants. These results show that neonatal PAE exposure alters the mRNA expression of placental MTs and FATP1, which are related to fetal growth and development.


Assuntos
Dietilexilftalato/toxicidade , Proteínas de Transporte de Ácido Graxo/biossíntese , Proteínas de Ligação a Ácido Graxo/biossíntese , Regulação da Expressão Gênica/efeitos dos fármacos , Exposição Materna/efeitos adversos , Metalotioneína/biossíntese , Placenta/metabolismo , Proteínas da Gravidez/biossíntese , RNA Mensageiro/biossíntese , Adulto , China , Feminino , Humanos , Recém-Nascido , Masculino , Gravidez
16.
FASEB J ; 28(11): 4617-28, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25103224

RESUMO

Cigarette smoke (CS) induces inflammatory responses characterized by increase of immune cells and cytokine release. Remodeling processes, such as mucus hypersecretion and extracellular matrix protein production, are also directly or indirectly induced by CS. Recently, we showed that activation of the exchange protein directly activated by cAMP (Epac) attenuates CS extract-induced interleukin (IL)-8 release from cultured airway smooth muscle cells. Using an acute, short-term model of CS exposure, we now studied the role of Epac1, Epac2, and the Epac effector phospholipase-Cε (PLCε) in airway inflammation and remodeling in vivo. Compared to wild-type mice exposed to CS, the number of total inflammatory cells, macrophages, and neutrophils and total IL-6 release was lower in Epac2(-/-) mice, which was also the case for neutrophils and IL-6 in PLCε(-/-) mice. Taken together, Epac2, acting partly via PLCε, but not Epac1, enhances CS-induced airway inflammation in vivo. In total lung homogenates of Epac1(-/-) mice, MUC5AC and matrix remodeling parameters (transforming growth factor-ß1, collagen I, and fibronectin) were increased at baseline. Our findings suggest that Epac1 primarily is capable of inhibiting remodeling processes, whereas Epac2 primarily increases inflammatory processes in vivo.


Assuntos
Remodelação das Vias Aéreas/fisiologia , Fatores de Troca do Nucleotídeo Guanina/metabolismo , Fumaça/efeitos adversos , Remodelação das Vias Aéreas/genética , Animais , Linhagem Celular , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Citocinas/metabolismo , Feminino , Fatores de Troca do Nucleotídeo Guanina/genética , Inflamação/metabolismo , Pulmão/metabolismo , Camundongos Endogâmicos C57BL , Camundongos Knockout
17.
Cell Signal ; 25(5): 1126-35, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23403124

RESUMO

The osteoblasts could be lead to the occurrence of apoptosis by oxidative stress. The zinc transporter family SLC30A (ZnTs) plays an important role in the regulation of zinc homeostasis, however, its function in apoptosis of MC3T3-E1 cells remains unknown. This study was aimed to investigate the role of zinc transporters in cell survival, particularly in MC3T3-E1 cells, during oxidative stress, and the molecular mechanism involved. Our study found that hydrogen peroxide can induce zinc-overloaded in the cells. While high concentration of zinc plays an important role in inducing apoptosis of the MC3T3-E1 cells, we demonstrated that ZnT7 can protect MC3T3-E1 cells and reduce the aggregation of intracellular free zinc ions as well as inhibit apoptosis induced by H2O2. Moreover, ZnT7 overexpression enhanced the anti-apoptotic effects. Interestingly, suppression of ZnT7 by siRNA could significantly exacerbate apoptosis in MC3T3-E1 cells. We also found that ZnT7 promotes cell survival via two distinct signaling pathways involving activation of the PI3K/Akt-mediated survival pathway and activation of MAPK/ERK pathway. Collectively, these results suggest that ZnT7 overexpression significantly protects osteoblasts cells from apoptosis induced by H2O2. This effect is mediated, at least in part, through activation of PI3K/Akt and MAPK/ERK pathways.


Assuntos
Apoptose , Proteínas de Transporte de Cátions/metabolismo , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Estresse Oxidativo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Animais , Apoptose/efeitos dos fármacos , Proteínas de Transporte de Cátions/antagonistas & inibidores , Proteínas de Transporte de Cátions/genética , Linhagem Celular , Peróxido de Hidrogênio/toxicidade , Camundongos , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais
18.
J Cancer Res Clin Oncol ; 138(11): 1865-77, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22736026

RESUMO

BACKGROUND: ß-Elemene, isolated from more than 50 Chinese herbs and plants, has shown promising anticancer effects against a broad spectrum of tumors, such as lung, breast, prostate, cervical, colon and ovarian carcinomas (Wang et al. in Cell Mol Life Sci 62:881-893, 2005; Li et al. in Cell Mol Life Sci 62:894-904, 2005; J Pharm Pharmacol 62(8):1018-1027, 2010). But it has not reported in osteosarcoma cells. The aim of the present study is to investigate the antitumor effect of ß-elemene on human osteosarcoma cancer cells and the molecular mechanism involved. RESULTS: ß-Elemene inhibited the viability of human osteosarcoma cells in a dose-time-dependent manner. The suppression of cell viability was due to the induction of apoptosis. Our study also found that ß-elemene treatment upregulated HIF-1α protein, which partially inhibits apoptosis. Knockdown of HIF-1α with small interfering RNA or co-treatment with the HIF-1α inhibitor YC-1 significantly enhanced the antitumor effects of ß-elemene. CONCLUSIONS: Our study first found that ß-elemene could increase the expression of HIF-1α through ROS and PI3K/Akt/mTor signaling pathway. And HIF-1α partially prevents human osteosarcoma cells from undergoing apoptosis. The anticancer effects of ß-elemene was weakened by HIF-1α. So, we recognize that a combination of ß-elemene with HIF-1α inhibitor might be a useful therapeutic option for osteosarcoma.


Assuntos
Apoptose/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Sesquiterpenos/farmacologia , Western Blotting , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cromonas/farmacologia , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/farmacologia , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia/antagonistas & inibidores , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Indazóis/farmacologia , Microscopia de Fluorescência , Morfolinas/farmacologia , Osteossarcoma/genética , Osteossarcoma/metabolismo , Osteossarcoma/patologia , Fosfatidilinositol 3-Quinases/metabolismo , Inibidores de Fosfoinositídeo-3 Quinase , Proteínas Proto-Oncogênicas c-akt/metabolismo , Interferência de RNA , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacos , Serina-Treonina Quinases TOR/metabolismo , Fatores de Tempo
20.
Biol Trace Elem Res ; 148(3): 420-9, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22434380

RESUMO

Zinc has been shown to increase bone mass and promote bone cell proliferation and differentiation. We, therefore, hypothesized that zinc might be cytoprotective for bone cells during oxidative stress. The cells were divided into H(2)O(2), zinc and zinc+H(2)O(2) groups. In the present study, zinc was found to inhibit H(2)O(2)-induced apoptosis in MC3T3-E1 cells, as shown by analysis of Annexin V/PI double staining. Western blot data showed that in zinc+H(2)O(2)-treated cells, zinc decreased the levels of AIF, Bax and active caspase-9 and -3, which are pro-apoptotic factors. And zinc inhibited release of cytochrome c from mitochondria to cytosol in zinc+H(2)O(2)-treated cells. Further investigation shows that protection is via activation of PI3K/Akt/mTor and MAPK /ERK pathways and inhibition of MAPK/P38 and MAPK/JNK pathways. Protecting osteoblast cells from oxidative damage presents a potential application in the treatment of osteoporosis.


Assuntos
Apoptose/efeitos dos fármacos , Peróxido de Hidrogênio/farmacologia , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Zinco/farmacologia , Animais , Linhagem Celular , Camundongos , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...