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1.
J Colloid Interface Sci ; 630(Pt B): 317-327, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36327734

RESUMO

Developing optimal catalysts, to suppress the shuttling of lithium polysulfides (LiPSs), and serving as bifunctional catalyst with fast discharge-charge reaction kinetics, are essential for the practical applications of Li-S batteries. Herein, based on density functional theory (DFT) calculations, single-atom catalysts formed by embedding 3d transition metals (TMs) into the nitrogen doped defective black phosphorus carbide (TM@N4-CP) are systematically explored toward fast kinetics in Li-S batteries. Remarkably, V@N4-CP, possessing excellent metallic features, outstanding structural stability, suitable binding and easy diffusion for LiPSs, eventually stands out as the promising bifunctional electrocatalyst. Our results unveil that d-p orbital hybridization between transition metal (TM) atom and sulfur species is accompanied by weakened surrounding Li-S bonds. Consequently, the formation of TM-S bonds not only ensures inhibition of LiPSs shuttling, but also promotes the dissociation of Li2S. With the analysis of correlation map of key parameters, the ICOHP values of TM-S bonds and adsorption energy of *Li2S are identified and proposed as descriptors for fast screening towards fast reaction kinetics. Our work shows a feasible strategy for the rational design and retrieval of the decisive feature of active catalysts for Li-S batteries.

2.
Sci Total Environ ; 855: 158924, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36152845

RESUMO

Mono(2-ethylhexyl) phthalate (MEHP) is a metabolite of DEHP which is one of phthalic acid esters (PAEs) widely used in daily necessities. Moreover, MEHP has been proven to have stronger biological toxicity comparing to DEHP. In particular, several recent population-based studies have reported that intrauterine exposure to MEHP results in adverse pregnancy outcomes. To explore the mechanisms and metabolic biomarkers of MEHP exposure, we examined the metabolic status of HTR-8/Svneo cell lines exposed to different doses of MEHP (0, 1.25, 5.0, 20 µM). Global and dose-response metabolomics tools were used to identify metabolic perturbations and sensitive markers associated with MEHP. Only 22 metabolic features (accounted for <1 %) were significantly changed when exposed to 1.25 µM. However, when the exposure dose was increased to 5 or 20 µM, the number of significantly changed metabolic features exceeded 300 (approximately 10 %). In particular, amino acid metabolism, pyrimidine metabolism and glutathione metabolism were widely affected according to the enrich analysis of those significant altered metabolites, which has and have previously been reported to be closely related to fetal development. Moreover, 5'-UMP and N-acetylputrescine with the lowest effective concentrations (EC-10 = 0.1 µM and EC+10 = 0.11 µM, respectively) were identified as sensitive endogenous biomarkers of MEHP exposure.


Assuntos
Dietilexilftalato , Ácidos Ftálicos , Gravidez , Feminino , Humanos , Dietilexilftalato/toxicidade , Dietilexilftalato/metabolismo , Trofoblastos/química , Trofoblastos/metabolismo , Ácidos Ftálicos/toxicidade , Ácidos Ftálicos/metabolismo , Biomarcadores/análise
3.
Water Res ; 226: 119279, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36323206

RESUMO

Solar-driven interfacial evaporation has enormous promise for fresh water recovery and salt harvesting, but salt accumulation-related challenges stand in its way. Herein, we report a spined groove-ridge pairs inspired by the shell ornamentation of the Vasticardium vertebratum, which addresses salt accumulation by artfully integrating salt reflux into localized salt crystallization. The seashell-mimetic radial V-groove array enables the 3D evaporator to transport water rapidly and directionally, resulting in high-performance water evaporation (∼95% efficiency) and localized crystallization. The periodic spines enlightened by the spine-bearing ridge on the seashell provide considerable micro-unit salt reflux. The 2-in-1 integration design endows the three-dimensional evaporator with superior solar-driven zero liquid discharge and excellent long-term salt resistance even when dealing with high-salinity brine (20 wt% NaCl) and a series of heavy metallic salt solutions. Our design offers a new alternative solution to avoiding salt scaling and could advance locally crystallized solar evaporators towards practical applications.


Assuntos
Purificação da Água , Animais , Purificação da Água/métodos , Exoesqueleto , Luz Solar , Cloreto de Sódio , Água , Cloreto de Sódio na Dieta , Impressão Tridimensional
4.
Front Immunol ; 13: 959417, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36341399

RESUMO

Recent evidence has gradually recognized that the immune and skeletal systems are two closely correlated systems, but the specific immune factors on bone mineral density (BMD) are largely unknown. Based on the summary-level data of genome-wide association studies (GWASs), we performed a series of analyses including two-sample Mendelian randomization (MR) analysis to test potential causal links between 731 immune traits [including median fluorescence intensities (MFIs), absolute cell (AC) counts, relative cell (RC) counts, and morphological parameters (MP)] and BMD. After false discovery rate (FDR) correction, 9 MFI-BMD, 16 AC-BMD, 22 RC-BMD, and 5 MP-BMD pairs reached the level of significance (FDR-adjusted p< 0.05). For MFI traits, the T- and B-cell panels had the largest number of significant immune trait pairs than other panels. CD40, as a molecule expressed by four subsets of monocytes, was highlighted due to its consistently positive correlation with BMD at four sites. For both AC and RC traits, immune traits from the T-cell panel were also highlighted, with CD39-positive T-cell subsets being the most frequently observed feature. For MP traits, the most significant association immune trait with BMD was SSC-A on CD14+ monocyte. Sensitivity analyses suggested that the identified immune factors were robust to pleiotropy. Multivariable MR analysis confirmed the independent causal effect of several immune traits on BMD. Mediation analyses showed that CD40 on monocytes could mediate multiple immune traits, especially the suggestive associations of CD27 on several memory B cells with BMD mediated by CD40 on CD14+ CD16- monocyte. Our study represents the first comprehensive evaluation of the causal effects of immune traits on the risk of osteoporosis. The findings highlighted the complex and important role of immune-derived factors in the pathogenesis of osteoporosis.


Assuntos
Densidade Óssea , Osteoporose , Humanos , Estudo de Associação Genômica Ampla , Polimorfismo de Nucleotídeo Único , Antígenos CD40 , Fatores Imunológicos
5.
Foods ; 11(21)2022 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-36359939

RESUMO

Rice production affects the food security and socioeconomic status of over half the world's population. Rice-producing countries, however, are facing population growth, reduction in rice planting area, and global change. Understanding the trends of rice production and major determinants is key to regulating rice production. We thus analyzed the trends of rice production and related determinants in China from 2001 to 2021, revealing that the annual rice production (TRP) has risen steadily (r = 0.929, p < 0.0001) in recent 20 years. TRP in 2021 was 19.9% higher than that in 2001, which was primarily achieved by the increment of middle rice production (MRP). MRP increased by 46.2% from 2000 to 2018, and grain yield per unit area (GPA) was the largest in middle rice. The enhancement of GPAs is significantly correlated with the consumption of agricultural resources and the number of released rice cultivars, but variations exist. TRP and GPA vary in different provinces; Hunan (25 ± 2 megatons) and Xinjiang (8364 ± 806 kg/hectare) show the largest values, respectively. TRP could be further increased by 13.8% by improving MRP. The results suggest that rice production in China has a large potential to be further improved through regulations.

7.
Nat Commun ; 13(1): 6577, 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36323699

RESUMO

17ß-hydroxysteroid dehydrogenase-13 is a hepatocyte-specific, lipid droplet-associated protein. A common loss-of-function variant of HSD17B13 (rs72613567: TA) protects patients against non-alcoholic fatty liver disease with underlying mechanism incompletely understood. In the present study, we identify the serine 33 of 17ß-HSD13 as an evolutionally conserved PKA target site and its phosphorylation facilitates lipolysis by promoting its interaction with ATGL on lipid droplets. Targeted mutation of Ser33 to Ala (S33A) decreases ATGL-dependent lipolysis in cultured hepatocytes by reducing CGI-58-mediated ATGL activation. Importantly, a transgenic knock-in mouse strain carrying the HSD17B13 S33A mutation (HSD17B1333A/A) spontaneously develops hepatic steatosis with reduced lipolysis and increased inflammation. Moreover, Hsd17B1333A/A mice are more susceptible to high-fat diet-induced nonalcoholic steatohepatitis. Finally, we find reproterol, a potential 17ß-HSD13 modulator and FDA-approved drug, confers a protection against nonalcoholic steatohepatitis via PKA-mediated Ser33 phosphorylation of 17ß-HSD13. Therefore, targeting the Ser33 phosphorylation site could represent a potential approach to treat NASH.


Assuntos
Hepatopatia Gordurosa não Alcoólica , Camundongos , Animais , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Hepatopatia Gordurosa não Alcoólica/genética , Hepatopatia Gordurosa não Alcoólica/metabolismo , Fosforilação , Serina/metabolismo , 17-Hidroxiesteroide Desidrogenases/genética , Hepatócitos/metabolismo , Fígado/metabolismo
8.
Artigo em Inglês | MEDLINE | ID: mdl-36424351

RESUMO

We present the first example of charged imidazolium functionalized porphyrin-based covalent organic framework (Co-iBFBim-COF-X) for electrocatalytic CO2 reduction reaction, where the free anions (e.g., F-, Cl-, Br-, and I-) of imidazolium ions nearby the active Co sites can stabilize the key intermediate *COOH and inhibit hydrogen evolution reaction. Thus, Co-iBFBim-COF-X exhibits higher activity than the neutral Co-BFBim-COF, following the trend of F- < Cl- < Br- < I-. Particularly, the Co-iBFBim-COF-I- showed nearly 100% CO2 selectivity at a low full-cell voltage of 2.3 V, and achieved a high CO2 partial current density of 52 mA cm-2 with a turnover frequency of 3018 h-1 at 2.4 V in the anion membrane electrode assembly, which is 3.57 times larger than that of neutral Co-BFBim-COF. This work provides new insight into the importance of free anions in the stabilization of intermediates and decreasing the local binding energy of H2O with active moiety to enhance CO2 reduction reaction.

9.
PLoS One ; 17(10): e0266988, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36264981

RESUMO

INTRODUCTION: With the widespread use of one-lung ventilation (OLV) in thoracic surgery, it is unclear whether maintenance anesthetics such as propofol and inhaled anesthetics are associated with postoperative complications. The purpose of this study was to compare the effects of propofol and inhaled anesthetics on postoperative complications in OLV patients. METHODS: PubMed, EMBASE, Medline, and Cochrane Library were searched for relevant randomized controlled trials until 09/2021. All randomized controlled trials comparing the effect of propofol versus inhaled anesthetics on postoperative complications in OLV patients were included. All randomized controlled trials comparing:(a) major complications (b) postoperative pulmonary complications (c) postoperative cognitive function (MMSE score) (d) length of hospital stay (e) 30-day mortality, were included. RESULTS: Thirteen randomized controlled trials involving 2522 patients were included in the analysis. Overall, there was no significant difference in major postoperative complications between the inhaled anesthetic and propofol groups (OR 0.78, 95%CI 0.54 to 1.13, p = 0.19; I2 = 0%). However, more PPCs were detected in the propofol group compared to the inhalation anesthesia group (OR 0.62, 95%CI 0.44 to 0.87, p = 0.005; I2 = 37%). Both postoperative MMSE score (SMD -1.94, 95%CI -4.87 to 0.99, p = 0.19; I2 = 100%) and hospital stay (SMD 0.05, 95%CI -0.29 to 0.39, p = 0.76; I2 = 73%) were similar between the two groups. The 30-day mortality rate was also not significantly different between groups (OR 0.79, 95%CI 0.03 to 18, p = 0.88; I2 = 63%). CONCLUSIONS: In patients undergoing OLV, general anesthesia with inhaled anesthetics reduced PPC compared to propofol, but did not provide clear benefits on other major complications, cognitive function, length of hospital stay, or mortality.


Assuntos
Anestésicos Inalatórios , Ventilação Monopulmonar , Propofol , Humanos , Propofol/efeitos adversos , Anestésicos Intravenosos/efeitos adversos , Sevoflurano , Anestésicos Inalatórios/efeitos adversos , Complicações Pós-Operatórias/induzido quimicamente , Ensaios Clínicos Controlados Aleatórios como Assunto
10.
Se Pu ; 40(10): 944-951, 2022 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-36222258

RESUMO

A new method for sample pretreatment using improved QuEChERs was established, and 289 organic pollutants with health risks could be identified and quantified through gas chromatography-orbitrap high-resolution mass spectrometry (GC-Orbitrap HRMS). A high-resolution database of 289 environmental pollutants belonging to ten categories, including organochlorine pesticides (OCPs), polycyclic aromatic hydrocarbons (PAHs), phthalates (PAEs), polychlorinated biphenyls (PCBs), and other agricultural chemicals (ACs), was established for the non-targeted screening and quantitative analysis. A simple method for biological sample preparation using improved QuEChERs was proposed by combining a conventional QuEChERs method and a column purification method. After purification using a Florisil column, the lipid content was reduced by 99.9%, which significantly reduced the interference of the matrix effect observed during the analysis. Furthermore, simultaneous high-accuracy qualitative screening and quantitative analysis of the target compounds were performed through high-resolution mass spectrometry (60000 resolution) conducted in the full scan mode. The limits of quantification were 0.56-57.8 pg/g, presenting a large linear range (~106), and the recovery range was 40%-120%. Due to the high-resolution and sensitivity of Q Exactive GC-Orbitrap HRMS, the limits of quantification of the target compounds were significantly lower than those achieved through methods based on conventional chromatography and mass spectrometry. Moreover, ultratrace organic contaminants that cannot be detected by conventional methods can be accurately quantified by the proposed method. Sea cucumber samples collected at the breeding site were analyzed using the proposed high-coverage multi-objective analytical method, and more than 100 types of organic pollutants were detected; the mean contents of PAHs, ACs, PAEs, and OCPs were 157.8, 153.2, 64.4, and 46.4 ng/g dw, respectively, which were higher than those of other pollutants. Some new contaminants, such as 9-chlorofluorene, 5-chloroacenaphthene, and 3-methylcholanthrene, were detected at very low contents for the first time in the sea cucumber samples. The proposed method is simple and efficient, allows the detection of pollutants at very low contents, and provides accurate and reliable results. Thus, this high-coverage multi-objective analytical method can be widely used for broad-spectrum screening and accurate quantification of contaminants in various aquatic products, providing technical support for food safety control.


Assuntos
Poluentes Ambientais , Hidrocarbonetos Clorados , Praguicidas , Bifenilos Policlorados , Hidrocarbonetos Policíclicos Aromáticos , Pepinos-do-Mar , Animais , Poluentes Ambientais/análise , Etilaminas , Cromatografia Gasosa-Espectrometria de Massas , Hidrocarbonetos Clorados/análise , Lipídeos , Espectrometria de Massas , Metilcolantreno/análise , Praguicidas/análise , Bifenilos Policlorados/análise , Hidrocarbonetos Policíclicos Aromáticos/análise
11.
Oncogene ; 41(48): 5199-5213, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36273042

RESUMO

Cancer cells rely on heightened protein quality control mechanisms, including the ubiquitin-proteosome system that is predominantly driven by ubiquitination comprising E1, E2, and E3 trienzyme cascades. Although E3s have been extensively studied, the implication of E2s in tumorigenesis is poorly defined. Here we reveal a critical E2 in the pathogenesis of hepatocellular carcinoma (HCC). Among all of E2s, UBE2O shows the strongest association with HCC survival prognosis, and its expression is increased in HCC tumors. Accordingly, UBE2O deficiency inhibits HCC growth and metastasis both in vitro and in vivo, while its overexpression has opposite effects. Depending on both E2 and E3 enzymatic activities, UBE2O can interact with and mediate the ubiquitination and degradation of HADHA, a mitochondrial ß-oxidation enzyme, thereby modulating lipid metabolic reprogramming. HADHA is reduced in HCC tumors and inversely correlated with UBE2O levels. Importantly, HADHA acts as a tumor suppressor and primarily mediates UBE2O's function on HCC. Moreover, liver-specific deletion of Ube2o in mice are resistant to DEN-induced hepatocarcinogenesis, along with HADHA upregulation and reduced hepatic lipid accumulation. These data reveal UBE2O as a novel oncogenic driver for metabolic reprogramming and HCC development, highlighting the potential of targeting UBE2O/HADHA axis for HCC therapy.

12.
BMC Ophthalmol ; 22(1): 392, 2022 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-36192712

RESUMO

BACKGROUND: During cataract phacoemulsification surgery, an Intrepid® balanced (IB) tip can achieve a larger amplitude, which may lead to higher energy efficiency than a Kelman (K) tip when paired with a torsional phaco platform. In this retrospective cohort study, we compared their energy efficiency and damage to the cornea under a new energy setting. METHODS: The medical records of 104 eyes of 79 patients were reviewed, with 47 eyes belonging to the IB group and 57 eyes belonging to the K group. All surgeries were performed on an Alcon Centurion® platform with gravity infiltration. Surgical parameters, visual outcome, central corneal thickness (CCT) changes, and endothelial cell density (ECD) loss rate were recorded and calculated. RESULTS: No significant differences in postoperative best corrected visual acuity (BCVA), intraocular pressure (IOP), total ultrasound time, estimated fluid aspirated, CCT changes, or ECD loss rate were observed between the two groups. We divided the included eyes into soft nucleus and hard nucleus subgroups and found lower cumulative dissipated energy (CDE, 8.15 ± 8.02 vs 14.82 ± 14.16, P = 0.023), cumulative torsional energy (CTE, 8.06 ± 7.87 vs 14.13 ± 13.02, P = 0.027), and cumulative longitudinal energy (CLE, 0.09 ± 0.17 vs 0.69 ± 1.37, P = 0.017) in the IB group than in the K group, implying less energy used and higher energy efficiency of the IB tip. CONCLUSION: Lower CLE in the IB group indicates fewer phaco tip obstructions and a significantly higher capability to conquer hard nuclei with IB tips with statistical significance. With an ultra-perfusion cannula, the balanced tip does not cause more corneal damage.


Assuntos
Facoemulsificação , Conservação de Recursos Energéticos , Endotélio Corneano , Humanos , Facoemulsificação/efeitos adversos , Estudos Prospectivos , Estudos Retrospectivos , Acuidade Visual
13.
Cell Death Dis ; 13(10): 893, 2022 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-36273194

RESUMO

Noninflammatory clearance of dying cells by professional phagocytes, termed efferocytosis, is fundamental in both homeostasis and inflammatory fibrosis disease but has not been confirmed to occur in chronic pancreatitis (CP). Here, we investigated whether efferocytosis constitutes a novel regulatory target in CP and its mechanisms. PRSS1 transgenic (PRSS1Tg) mice were treated with caerulein to mimic CP development. Phospholipid metabolite profiling and epigenetic assays were performed with PRSS1Tg CP models. The potential functions of Atp8b1 in CP model were clarified using Atp8b1-overexpressing adeno-associated virus, immunofluorescence, enzyme-linked immunosorbent assay(ELISA), and lipid metabolomic approaches. ATAC-seq combined with RNA-seq was then used to identify transcription factors binding to the Atp8b1 promoter, and ChIP-qPCR and luciferase assays were used to confirm that the identified transcription factor bound to the Atp8b1 promoter, and to identify the specific binding site. Flow cytometry was performed to analyze the proportion of pancreatic macrophages. Decreased efferocytosis with aggravated inflammation was identified in CP. The lysophosphatidylcholine (LPC) pathway was the most obviously dysregulated phospholipid pathway, and LPC and Atp8b1 expression gradually decreased during CP development. H3K27me3 ChIP-seq showed that increased Atp8b1 promoter methylation led to transcriptional inhibition. Atp8b1 complementation substantially increased the LPC concentration and improved CP outcomes. Bhlha15 was identified as a transcription factor that binds to the Atp8b1 promoter and regulates phospholipid metabolism. Our study indicates that the acinar Atp8b1/LPC pathway acts as an important "find-me" signal for macrophages and plays a protective role in CP, with Atp8b1 transcription promoted by the acinar cell-specific transcription factor Bhlha15. Bhlha15, Atp8b1, and LPC could be clinically translated into valuable therapeutic targets to overcome the limitations of current CP therapies.


Assuntos
Adenosina Trifosfatases , Lisofosfatidilcolinas , Macrófagos , Pancreatite Crônica , Animais , Camundongos , Células Acinares/metabolismo , Adenosina Trifosfatases/genética , Adenosina Trifosfatases/metabolismo , Ceruletídeo/toxicidade , Histonas/metabolismo , Inflamação/metabolismo , Lisofosfatidilcolinas/genética , Lisofosfatidilcolinas/metabolismo , Macrófagos/metabolismo , Pancreatite Crônica/induzido quimicamente , Pancreatite Crônica/genética , Pancreatite Crônica/metabolismo , Proteínas de Transferência de Fosfolipídeos/genética , Proteínas de Transferência de Fosfolipídeos/metabolismo , Fatores de Transcrição/metabolismo
14.
Front Plant Sci ; 13: 983576, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119594

RESUMO

High concentration of tropospheric ozone (O3) causes crop yield losses, which could be reduced by foliar application of ethylenediurea (EDU). Rice grain appearance is a major quality trait that determines the milling quality, white rice productivity and the market value. Grain chalkiness is one of the common defects that deteriorate the grain appearance in rice due to its negative effects on palatability and milling yield. Whether EDU could reduce grain chalkiness in rice which was usually increased by high concentration of O3 is not clarified. We report the grain chalkiness in 19 rice cultivars (CVs) of three types: indica (6 CVs), japonica (5 CVs) and hybrids (8 CVs), observed in an EDU application experiment in the field in China. The ambient O3 level as expressed by accumulated hourly O3 concentration over the threshold of 40 ppb (AOT40) for 80 days until maturity reached 12.8 ppm h at a near-by monitoring station. Fraction of the chalky grains (FCG) in the hybrid cultivars was 8% lower in EDU than that in the control treatments, whereas no significant effect of EDU on FCG was found in japonica or indica cultivars. The reduction of FCG due to EDU treatment in hybrid cultivars was attributed to the significant reduction of milky white grains followed by that of white belly grains. Thus, the application of EDU could ameliorate the decline of grain appearance quality in hybrid rice by decreasing the FCG and enhancing the fraction of perfect grains (FPG). Moreover, there were significant interactions between the EDU application and rice cultivars, indicating varietal difference in the protection of grain appearance quality by EDU. These results suggest the need for further studies on the mechanisms of the effects of EDU on grain chalkiness.

15.
Front Public Health ; 10: 905178, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36091497

RESUMO

Purpose: Osteoporosis is associated with metabolic alterations, but the causal roles of serum metabolites on osteoporosis have not been identified. Methods: Based on the large individual-level datasets from UK Biobank as well as GWAS summary datasets, we first constructed genetic risk scores (GRSs) for 308 of 486 human serum metabolites and evaluated the effect of each GRS on 2 major osteoporosis phenotypes, i.e., estimated bone miner density (eBMD) and fracture, respectively. Then, two-sample Mendelian Randomization (MR) was performed to validate the casual metabolites on osteoporosis. Multivariable MR analysis tested whether the effects of metabolites on osteoporosis are independent of possible confounders. Finally, we conducted metabolic pathway analysis for the metabolites involved in bone metabolism. Results: We identified causal effects of 18 metabolites on eBMD and 1 metabolite on fracture with the GRS method after adjusting for multiple tests. Then, 9 of them were further validated with MR as replication, where comprehensive sensitive analyses proved robust of the causal associations. Although not identified in GRS, 3 metabolites were associated with at least three osteoporosis traits in MR results. Multivariable MR analysis determined the independent causal effect of several metabolites on osteoporosis. Besides, 23 bone metabolic pathways were detected, such as valine, leucine, isoleucine biosynthesis (p = 0.053), and Aminoacyl-tRNA biosynthesis (p = 0.076), and D-glutamine and D-glutamate metabolism (p = 0.004). Conclusions: The systematic causal analyses strongly suggested that blood metabolites have causal effects on osteoporosis risk.


Assuntos
Análise da Randomização Mendeliana , Osteoporose , Estudo de Associação Genômica Ampla , Humanos , Osteoporose/genética , Osteoporose/metabolismo , Polimorfismo de Nucleotídeo Único , Fatores de Risco
16.
Artigo em Inglês | MEDLINE | ID: mdl-36165706

RESUMO

OBJECTIVES: Long noncoding RNAs (lncRNAs) play important roles in rheumatoid arthritis (RA) pathogenesis. However, specific lncRNAs that regulate gene expression in RA pathogenesis are poorly known. This study was undertaken to characterize a novel lncRNA (lnc-RNU12) that has a lower-than-normal expression level in RA patients. METHODS: We performed initial genome-wide lncRNA microarray screening in peripheral blood mononuclear cells (PBMCs) of 28 RA cases and 18 controls. Multiple methods were used to validate the detected associations between lncRNAs and RA. Furthermore, we identified the source and characteristics of the highlighted lncRNAs, detected the target genes, and determined the functional effect on immune cells through lncRNA knockdown in Jurkat T cell lines. RESULTS: lnc-RNU12 was downregulated in PBMCs and T cell subtypes of RA patients and genetically associated with RA risk. lnc-RNU12 mediates the effect of microbiome alterations on RA risk. Activation of T cells caused low expression of lnc-RNU12. Knockdown of lnc-RNU12 in Jurkat T cells caused cell cycle S-phase arrest and altered the expression of protein-coding genes related to the cell cycle and apoptosis (e.g. c-JUN, CCNL2, CDK6, MYC, RNF40, PKM, VPS35, DNAJB6 and FLCN). Finally, c-JUN and CCNL2 were identified as target genes of lnc-RNU12 at the mRNA and protein expression levels. RNA-binding protein immunoprecipitation assays verified the interaction between lnc-RNU12 and the two proteins (c-Jun and cyclin L2) in Jurkat cells. CONCLUSION: Our study suggested that lnc-RNU12 was involved in the pathogenesis of RA by influencing the T cell cycle by targeting c-JUN and CCNL2.

17.
Acc Chem Res ; 55(20): 2978-2997, 2022 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-36153952

RESUMO

ConspectusThe continuing increase of the concentration of atmospheric CO2 has caused many environmental issues including climate change. Catalytic conversion of CO2 using thermochemical, electrochemical, and photochemical methods is a potential technique to decrease the CO2 concentration and simultaneously obtain value-added chemicals. Due to the high energy barrier of CO2 however, this method is still far from large-scale applications which requires high activity, selectivity, and stability. Therefore, development of efficient catalysts to convert CO2 to different products is urgent. With their well-engineered pores and chemical compositions, high surface area, elevated CO2 adsorption capability, and adjustable active sites, porous crystalline frameworks including metal-organic frameworks (MOFs) and covalent organic frameworks (COFs) are potential materials for catalytic CO2 conversion. Here, we summarize our recent work on MOFs and COFs for thermocatalytic, electrocatalytic, and photocatalytic CO2 conversion and describe the structure-activity relationships that could guide the design of effective catalysts.The first section of this paper describes imidazolium-functionalized porous MOFs, including porous liquid and cationic MOFs with nucleophilic halogen ions, which can promote thermocatalytically CO2 cycloaddition reaction with epoxides toward cyclic carbonates at one bar pressure. A porous liquid MOF takes on the role of a CO2 reservoir to tackle the low local CO2 concentrations in gas-liquid-solid heterogeneous reactions. Imidazolium-functionalized MOFs with halogen ions for CO2 cycloaddition could avoid the use of cocatalysts, and this leads to milder and more facile experimental conditions and separation processes.In a section dealing with the electrocatalytic CO2 reduction reaction (CO2RR), we developed a series of conductive porous framework materials with fast electron transmission capabilities, which afford high current densities and outperform the traditional MOF and COF catalysts that have been reported. The intrinsically conductive two-dimensional 2D MOFs and COFs nanosheets based on the fully π-conjugated phthalocyanine motif with excellent electron transport capability were prepared, and strong electron transporters were also integrated into metalloporphyrin-based COFs for CO2RR. Cu2O quantum dots and Cu nanoparticles (NPs) can be uniformly dispersed on porous conductive MOFs/COFs to afford synergistic and/or tandem electrocatalysts, which can achieve highly selective production of CH4 or C2H4 in CO2RR.A third section describes our efforts to facilitate electron-hole separation in CO2 photocatalysis. Our focus is on regulation of coordination spheres in MOFs, fabrication of the architecture of MOF heterojunctions, and engineering MOF films to facilitate photocatalytic CO2 reduction.Finally, we discuss several problems associated with the studies of MOFs and COFs for CO2 conversion and consider some prospects of the fabrication of effective porous frameworks for CO2 adsorption and conversion.

18.
Adv Mater ; 34(44): e2201768, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36134533

RESUMO

Metal-organic frameworks (MOFs) with well-defined porous structures and tailored functionalities have been widely used in chemical sensing. However, the integration of MOFs with flexible electronic devices for wearable sensing is challenging because of their low electrical conductivity and fragile mechanical properties. Herein, a wearable sweat sensor for metabolite detection is presented by integrating an electrically conductive Ni-MOF with a flexible nanocellulose substrate. The MOF-based layered film sensor with inherent conductivity, highly porous structure, and active catalytic properties enables the selective and accurate detection of vitamin C and uric acid. More importantly, the lightweight sensor can conformably self-adhere to sweaty skin and exhibits high water-vapor permeability. Furthermore, a wireless epidermal nutrition tracking system for the in situ monitoring of the dynamics of sweat vitamin C is demonstrated, the results of which are comparable to those tested by high-performance liquid chromatography. This study opens a new avenue for integrating MOFs as the active layer in wearable electronic devices and holds promise for the future development of high-performance electronics with enhanced sensing, energy production, and catalytic capabilities through the implementation of multifunctional MOFs.


Assuntos
Estruturas Metalorgânicas , Dispositivos Eletrônicos Vestíveis , Suor/química , Adesivos , Ácido Ascórbico/análise
19.
J Clin Hypertens (Greenwich) ; 24(11): 1427-1435, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36134478

RESUMO

The aim of this study was to analyze the relationship between brachial-ankle pulse wave velocity (b-a PWV) and white coat effect (WCE), that is the difference between the elevated office blood pressure (BP) and the lower mean daytime pressure of ambulatory BP, in a mixed population of normotention, untreated sustained hypertension, sustained controlled hypertension, sustained uncontrolled hypertension, white coat hypertension, white coat uncontrolled hypertension. A total of 444 patients with WCE for systolic BP (54.1% female, age 61.86 ± 13.3 years) were enrolled in the study. Patients were separated into low WCE (<9.5 mm Hg) and high WCE (≥9.5 mm Hg) according to the median of WCE. The subjects with a high WCE showed a greater degree of arterial stiffness than those with a low WCE for systolic BP values (P < .05). The b-a PWV were 17.2 ± 3.3 m/s and 18.4 ± 3.4 m/s in low WCE and high WCE, respectively. The b-a PWV increased with the increase of WCE, showing a positive correlation between them (P > .05 for non-linearity). The significant association between the high WCE and the b-a PWV was confirmed by the results of multiple regression analysis after adjusting for confounding factors (ß = .78, 95% Cl .25-1.31, P = . 004). Similar results were observed in subgroups. In conclusion, WCE is significantly associated with arterial stiffness. More research is needed to determine the WCE and target organ damage.


Assuntos
Hipertensão , Rigidez Vascular , Hipertensão do Jaleco Branco , Humanos , Feminino , Pessoa de Meia-Idade , Idoso , Masculino , Pressão Sanguínea/fisiologia , Hipertensão do Jaleco Branco/diagnóstico , Hipertensão do Jaleco Branco/epidemiologia , Hipertensão do Jaleco Branco/complicações , Hipertensão/diagnóstico , Hipertensão/epidemiologia , Hipertensão/complicações , Índice Tornozelo-Braço , Análise de Onda de Pulso , Monitorização Ambulatorial da Pressão Arterial
20.
Sci Rep ; 12(1): 13613, 2022 08 10.
Artigo em Inglês | MEDLINE | ID: mdl-35948587

RESUMO

In this paper, three monitoring sections were set up in Lugu Lake, and water samples were collected in 2019, 2020, and 2021 for the determination of physical and chemical properties such as permanganate index, chemical oxygen demand, biochemical oxygen demand (BOD5) and so on. By using the single factor pollution index method and the Nemerow pollution index method, the water quality of three monitoring sections and the whole Lugu Lake was assessed, and the temporal and spatial changes of water quality were analyzed. The findings demonstrate that Lugu Lake's overall water quality is excellent, and that it has not altered significantly in three years.The results of evaluating the water quality by the single factor pollution index method show that, in the past three years, the water quality of the three monitoring sections and the whole of Lugu Lake is Category I, which belongs to no pollution, and the measured indicators all meet the water quality standard of Category I. It can be seen from the evaluation results of the Nemerow index method that the water quality pollution index of Lugu Lake is between 0.22 and 0.34 in the past three years and the water quality evaluation of Changdao Bay, Lake center, Zhaojia Bay and the whole are Category I standards in 2019, 2020 and 2021. In terms of time changes, the water quality of Lugu Lake has remained stable between 2019 and 2021, and the water quality has been good. From the perspective of spatial changes, in 2019 and 2020, the water quality in Lake center is better than the monitoring sections of Changdao Bay and Zhaojia Bay.


Assuntos
Lagos , Poluentes Químicos da Água , China , Monitoramento Ambiental/métodos , Lagos/química , Poluentes Químicos da Água/análise , Poluição da Água/análise , Qualidade da Água
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