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1.
J Clin Lab Anal ; : e23221, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32060969

RESUMO

BACKGROUND: Growing evidence suggested that B- and T-lymphocyte attenuator (BTLA) polymorphisms raised the susceptibility to a wide range of cancers. This study aimed to evaluate whether BTLA variants were related to the risk of esophageal squamous cell carcinoma (ESCC). METHODS: A total of 721 ESCC patients and 1208 matched non-cancer controls were included in this research, and four tagging BTLA polymorphisms (rs2171513 G > A, rs3112270 A > G, rs1982809 G > A, and rs16859629 T > C) were selected and genotyped using SNPscan™ Assays. RESULTS: In the present study, no significant relationship between BTLA polymorphisms and ESCC was observed. However, stratified analyses suggested that the variant of BTLA rs3112270 A > G reduced the risk of ESCC in the male subgroup (AG vs AA: adjusted OR = 0.78, 95% CI = 0.61-0.99, P = .042), BMI < 24 kg/m2 subgroup (AG vs AA: adjusted OR = 0.72, 95% CI = 0.55-0.93, P = .012; AG/GG vs AA: adjusted OR = 0.77, 95% CI = 0.60-0.98, P = .032), and ever drinking subgroup (AG vs AA: adjusted OR = 0.61, 95% CI = 0.38-0.97, P = .037). But when stratified by BMI ≥ 24 kg/m2 , the rs3112270 A > G polymorphism increased the susceptibility to ESCC (GG vs AA: adjusted OR = 1.91, 95% CI = 1.02-3.59, P = .045). Besides, we demonstrated that BTLA rs2171513 G > A polymorphism was protective of ESCC in the ever drinking subgroup (GA/AA vs GG: adjusted OR = 0.62, 95% CI = 0.39-0.97, P = .037). CONCLUSION: Taken together, our initial investigation postulated that the rs3112270 A > G and rs2171513 G > A variants in the BTLA gene are candidates for the risk of ESCC, which might be helpful for the early diagnosis and treatment of ESCC.

3.
Small ; : e1906735, 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31984632

RESUMO

The development of rechargeable metal-air batteries and water electrolyzers are highly constrained by electrocatalysts for the oxygen reduction reaction (ORR), oxygen evolution reaction (OER), and hydrogen evolution reaction (HER). However, the construction of efficient trifunctional electrocatalysts for ORR/OER/HER are highly desirable yet challenging. Herein, hollow carbon nanotubes integrated single cobalt atoms with Co9 S8 nanoparticles (CoSA + Co9 S8 /HCNT) are fabricated by a straightforward in situ self-sacrificing strategy. The structure of the CoSA + Co9 S8 /HCNT are verified by X-ray absorption spectroscopy and aberration-corrected scanning transmission electron microscopy. Theoretical calculations and experimental results embrace the synergistic effects between Co9 S8 nanoparticles and single cobalt atoms through optimizing the electronic configuration of the CoN4 active sites to lower the reaction barrier and facilitating the ORR, OER, and HER simultaneously. Consequently, rechargeable liquid and all-solid-state flexible Zn-air batteries based on CoSA + Co9 S8 /HCNT exhibit remarkable stability and excellent power density of 177.33 and 51.85 mW cm-2 , respectively, better than Pt/C + RuO2 counterparts. Moreover, the as-fabricated Zn-air batteries can drive an overall water splitting device assembled with CoSA + Co9 S8 /HCNT and achieve a current density of 10 mA cm-2 at a low voltage of 1.59 V, also superior to Pt/C + RuO2 . Therefore, this work presents a promising approach to an efficient trifunctional electrocatalyst toward practical applications.

4.
J Am Chem Soc ; 142(5): 2404-2412, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31902210

RESUMO

Atomically dispersed transition metal active sites have emerged as one of the most important fields of study because they display promising performance in catalysis and have the potential to serve as ideal models for fundamental understanding. However, both the preparation and determination of such active sites remain a challenge. The structural engineering of carbon- and nitrogen-coordinated metal sites (M-N-C, M = Fe, Co, Ni, Mn, Cu, etc.) via employing new heteroatoms, e.g., P and S, remains challenging. In this study, carbon nanosheets embedded with nitrogen and phosphorus dual-coordinated iron active sites (denoted as Fe-N/P-C) were developed and determined using cutting edge techniques. Both experimental and theoretical results suggested that the N and P dual-coordinated iron sites were favorable for oxygen intermediate adsorption/desorption, resulting in accelerated reaction kinetics and promising catalytic oxygen reduction activity. This work not only provides efficient way to prepare well-defined single-atom active sites to boost catalytic performance but also paves the way to identify the dual-coordinated single metal atom sites.

5.
J Cell Mol Med ; 24(1): 605-617, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31657881

RESUMO

The transition from non-muscle-invasive bladder cancer (NMIBC) to muscle-invasive bladder cancer (MIBC) is detrimental to bladder cancer (BLCA) patients. Here, we aimed to study the underlying mechanism of the subtype transition. Gene set variation analysis (GSVA) revealed the epithelial-mesenchymal transition (EMT) signalling pathway with the most positive correlation in this transition. Then, we built a LASSO Cox regression model of an EMT-related gene signature in BLCA. The patients with high risk scores had significantly worse overall survival (OS) and disease-free survival (DFS) than those with low risk scores. The EMT-related gene signature also performed favourably in the accuracy of prognosis and in the subtype survival analysis. Univariate and multivariate Cox regression analyses demonstrated that the EMT-related gene signature, pathological N stage and age were independent prognostic factors for predicting survival in BLCA patients. Furthermore, the predictive nomogram model was able to effectively predict the outcome of BLCA patients by appropriately stratifying the risk score. In conclusion, we developed a novel EMT-related gene signature that has tumour-promoting effects, acts as a negative independent prognostic factor and might facilitate personalized counselling and treatment in BLCA.

6.
J Hazard Mater ; 384: 121365, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31593863

RESUMO

Selenium (Se) is an essential nutrient for human beings at trace concentrations, but also a hazardous contaminant at high concentrations. As an important geological adsorbent, the transformation of 2-line ferrihydrite (Fh) strongly influences the geochemical behavior of selenium. However, little is known about the effect of the recrystallization of Fh on the fate of adsorbed Se(IV) in the reducing environments. We investigated the redistribution and transformation of Se(IV) during the recrystallization of Se(IV)-adsorbed Fh accelerated by Fe(II) under anaerobic conditions. Synchrotron based X-ray absorption near edge structure (XANES) spectroscopy was utilized to characterize oxidation state of Se. Results revealed that the adsorbed Se(IV) inhibited the Fe(II)-catalyzed recrystallization of ferrihydrite to goethite. Transmission electron microscopy (TEM) images showed that pH and the presence of Se(IV) had significant impacts on the morphology of the produced goethite. Approximately 30-75% adsorbed Se(IV) transformed to phosphate-unextractable form, indicating that the adsorbed Se transformed to more stable phase during the recrystallization of Fh. The XANES results indicated that a small fraction of Se(IV) was reduced to elemental Se. Our study demonstrated that the stability of adsorbed Se(IV) on ferrihydrite could be enhanced during Fe(II)-catalytic transformation of Fh under anoxic environments.

7.
J Biomed Mater Res B Appl Biomater ; 108(2): 343-352, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31013399

RESUMO

Optimal function of immunoisolated islets requires adequate supply of oxygen to metabolically active insulin producing beta-cells. Using mathematical modeling, we investigated the influence of the pO2 on islet insulin secretory capacity and evaluated conditions that could lead to the development of tissue anoxia, modeled for a 300 µm islet in a 500 µm microcapsule or a 500 µm planar, slab-shaped macrocapsule. The pO2 was used to assess the part of islets that contributed to insulin secretion. Assuming a 500 µm macrocapsule with a 300 µm islet, with oxygen consumption rate (OCR) of 100-300 nmol min-1 mg-1 DNA, islets did not develop any necrotic core. The nonfunctional zone (with no insulin secretion if pO2 < 0.1 mmHg) was 0.3% for human islets (OCR ~100 nmol/min/mg DNA) and 35% for porcine islets (OCR ~300 nmol/min/mg DNA). The OCR of the islet preparation is profoundly affected by islet size, with optimal size of <250 µm in diameter (human) or <150 µm (porcine). Our data suggest that microcapsules afford superior oxygen delivery to encapsulated islets than macrocapsules, and optimal islet function can be achieved by encapsulating multiple, small (<150 µm) islets with OCR of ~100 nmol min-1 mg-1 DNA (human islets) or ~200 nmol min-1 mg-1 DNA (porcine islets).

8.
Shanghai Kou Qiang Yi Xue ; 28(4): 402-407, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31792482

RESUMO

PURPOSE: To investigate the clinical outcomes and prognosis of harmonic scalpel assisted extracapsular dissection for benign parotid tumors. METHODS: A total of 132 patients with full medical archives who were diagnosed as benign parotid tumors from 3 medical centers were retrospectively analyzed. The included patients were divided into 3 groups according to tumor positions, namely, superficial pre-auricular, superficial tail, and deep lobe. The intra- and peri-operative variables, complications, functional outcomes and recurrence rates were analyzed with SPSS 16.0 software package. RESULTS: The surgical and drainage time were longer and the blood loss and drainage amount were significantly greater in deep lobe group (P<0.01). Facial nerve damage occurred more frequently in deep lobe group (P=0.022), while damages of great auricular nerve and parotid fascia, as well as capsule rupture were similar among 3 groups (P>0.05). More patients with decreased sensation or sialocele were observed in deep lobe group (P=0.004, 0.043, respectively), and no significant differences were found for wound infection and Frey's syndrome among 3 groups (P>0.05).Transient facial nerve paralysis occurred more frequently in deep lobe group (P<0.01), while permanent facial nerve paralysis was similar (P=0.052). No significant difference was observed for tumor recurrence (P=1.000). CONCLUSIONS: HS-ECD displays good outcomes and prognosis for benign tumor of parotid gland, with improved esthetic and functional results as well as fewer complications and recurrence rate postoperatively.


Assuntos
Neoplasias Parotídeas , Estética Dentária , Humanos , Recidiva Local de Neoplasia , Glândula Parótida , Complicações Pós-Operatórias , Estudos Retrospectivos
9.
Onco Targets Ther ; 12: 9435-9447, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31807022

RESUMO

Purpose: The aim of this study was to investigate the effects of gain-of-function (GOF) E76K-mutant Src homology-2 domain containing protein tyrosine phosphatase-2 (SHP2) on the biological behaviors of glioblastoma (GBM) cells, and explore the molecular mechanisms of GBM progression. Methods: Firstly, a negative control vector and vectors overexpressing SHP2 and E76K-mutant SHP2 were transduced into GBM cells (U87 and A172) using a lentivirus. The effect of GOF-mutant SHP2 on proliferation was measured using the MTT assay, flow cytometry, colony formation assay, and soft agar assay. Moreover, the migration and invasion of GBM cells were determined through the transwell assay. Related proteins of the extracellular signal-regulated kinase/cAMP response element binding protein (ERK/CREB) pathway were detected by Western blotting analysis. A xenograft model was established to confirm the tumor-promoting effect of GOF-mutant SHP2 in vivo. Finally, ERK was inhibited using a mitogen-activated protein kinase/ERK kinase inhibitor (U0126) to further explore the molecular mechanism of GOF-mutant SHP2 affecting GBM cells. Results: After transduction, the expression of SHP2 in the SHP2-mutant and SHP2-overexpression groups was higher than that observed in the control and normal groups. Our data indicated that GOF-mutant SHP2 enhanced the abilities of GBM cells for proliferation, migration, and invasion in vitro, and promoted tumor growth in vivo. Mechanistically, the ERK/CREB pathway was activated, and the levels of relevant proteins were increased in the SHP2-mutant group. Furthermore, following inhibition of ERK in the GOF-SHP2 mutant group, the activation of CREB was also depressed, and the malignant biological behaviors were weakened accordingly. Conclusion: The GOF-mutant SHP2 promoted GBM cell proliferation, metastasis, and tumor growth through the ERK/CREB pathway, providing a promising target for the treatment of GBM.

10.
Onco Targets Ther ; 12: 10343-10360, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819518

RESUMO

Non-small cell lung cancer (NSCLC) is frequently associated with oncogenic driver mutations, which play an important role in carcinogenesis and cancer progression. Targeting epidermal growth factor receptor (EGFR) mutations and anaplastic lymphoma kinase rearrangements has become standard therapy for patients with these aberrations because of the greater improvement of survival, tolerance, and quality-of-life compared to chemotherapy. Clinical trials for emerging therapies that target other less common driver genes are generating mixed results. Here, we review the literature on rare drivers in NSCLC with frequencies lower than 5% (e.g., ROS1, RET, MET, BRAF, NTRK, HER2, NRG1, FGFR1, PIK3CA, DDR2, and EGFR exon 20 insertions). In summary, targeting rare oncogenic drivers in NSCLC has achieved some success. With the development of new inhibitors that target these rare drivers, the spectrum of targeted therapy has been expanded, although acquired resistance is still an unavoidable problem.

11.
Mar Drugs ; 17(12)2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31847202

RESUMO

In recent years, the wide application of exopolysaccharides (EPSs) in food, cosmetics, medicine, and other fields has drawn tremendous attention. In this study, an EPS produced by Pseudoalteromonas agarivorans Hao 2018 was isolated and purified, and its fermentation conditions were optimized. Its structure and biological functions were also studied. The purity and molecular weight of EPS were determined by high performance liquid chromatography (HPLC), and the EPS exhibited a number average of 2.26 × 105 and a weight average of 2.84 × 105. EPS has good adsorption for Cu2+ and Pb2+. The adsorption rates can reach up to 69.79% and 82.46%, respectively. The hygroscopic property of EPS was higher than that of chitosan, but slightly lower than that of sodium hyaluronate. However, the water-retaining activity of EPS was similar to that of chitosan and sodium hyaluronate. EPS has strong ability to scavenge free radicals, including OH radical and O2- radical. Further, its activity on O2- radicals has similarities with that of vitamin C. EPS has broad application prospects in many fields, such as cosmetics, environmental protection.

12.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4111-4115, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872685

RESUMO

The planting area of Chinese medicinal materials is an important basis for formulating policies such as production and poverty alleviation of Chinese medicinal materials and is determining the quantity of medicinal materials trade. Accurately mastering the information of the distribution,area and yield of Chinese medicinal materials cultivation is the basis of the adjustment of the planting structure of traditional Chinese medicine. It is now the largest planting place of Mongolian traditional Chinese medicinal materials in Naiman banner that is belonging to Tongliao city,Inner Mongolia. It is of great significance to obtain the planting area of Mongolian Chinese medicinal materials in Naiman banner in time and effectively for the development of subsequent industries. In this study,Saposhnikovia divaricata,a medicinal plant planted in Naiman banner,was selected as an example,and the fusion 2 m resolution ZY-3 remote sensing image was used as the data source. Based on the ground survey data,the sample data of each typical ground object were selected,and the spectral characteristic curves of different ground objects were obtained,and the S. divaricata spectral information was obtained. Using the filtering texture analysis method based on probability statistics,five kinds of texture image display results under different texture filtering were compared and analyzed,and finally the S. divaricata texture features based on information entropy are determined. The distribution range and planting area of S. divaricata in Naiman banner were extracted and interpreted by using the texture and spectral information of remote sensing images. The results showed that: S. divaricata was mainly distributed in the northeast and central south of Naiman banner,and the planting area was 5 336 mu( 1 mu≈667 m2). The field verification data were in good agreement with the remote sensing interpretation results,and the difference was small. It shows that the combination of spectral information and texture information can realize the discrimination of S. divaricata,and the interpretation results can provide a reference for the county to formulate the poverty alleviation action of Chinese medicinal material industry and the economic development plan of agricultural producing areas.


Assuntos
Apiaceae , Medicina Tradicional Chinesa , Plantas Medicinais , Agricultura , China
13.
Huan Jing Ke Xue ; 40(12): 5330-5339, 2019 Dec 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854604

RESUMO

To understand the runoff pollution characteristics of the typical purple soil small watershed in the Three Gorges Reservoir (TGR), the rainfall-runoff duration under typical land use types of the Xinzheng watershed and the nitrogen (N) and phosphorus (P) concentrations in the outlet runoff of each catchment were dynamically monitored, and the loss rule of N and P in the rainfall runoff in the small watershed in the TGR was investigated and analyzed. The results showed that the losses of runoff N and P were 13.69 kg·(hm2·a)-1 and 1.50 kg·(hm2·a)-1, respectively. N and P in agricultural fertilizers and rainfall scouring were the main causes of runoff pollution in the Xinzheng watershed. The average concentration of total nitrogen (TN) and total phosphorus (TP) reached 10.05 mg·L-1 and 1.10 mg·L-1, far exceeding the occurrence standard of eutrophication, which should be cause for concern. The nitrate nitrogen (NN) and particulate phosphorus (PP) in the rainfall were 69.47 kg and 6.83 kg on August 15, 2010, accounting for 53.91% and 53.78% of TN and TP, respectively. The NN and AN were 6.68 kg and 5.61 kg, respectively, in the rainfall on August 26, 2010, accounting for 37.74% and 31.69% of TN and PP was 1.36 kg, accounting for 57.63% of TP, indicating that nitrogen loss was mainly through the soluble state, while phosphorus migration was dominated by particulate matter. Heavy rainfall in the Xinzheng watershed had a significant impact on the loss of nitrogen and phosphorus. This was related to the properties of purple soil, such as thin soil layer, frequent cultivation and relatively loose soil.

14.
Chem Commun (Camb) ; 55(98): 14805-14808, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31763647

RESUMO

We report a multi-shelled two-dimensional metal-organic framework (MOF), which is transferred to a Co/Ni-embedded bimetallic N-doped porous carbon. The bimetallic N-doped porous carbon exhibits high electrocatalytic activity and long-term stability toward the oxygen-reduction reaction (ORR) and Zn-air batteries.

15.
Biosci Rep ; 39(12)2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31763668

RESUMO

Spinal cord injury (SCI) often occurs in young and middle-aged population. The present study aimed to clarify the function of Galectin-3 (Gal-3) in neuroinflammation of SCI. Sprague-Dawley (SD) rat models with SCI were established in vivo. PC12 cell model in vitro was induced by lipopolysaccharide (LPS). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Gene chip were used to analyze the expression levels of genes in the signaling pathway. Histological assessment, ELISA and Western blotting were conducted to evaluate the effects of Gal-3 upon the SCI model. In the in vivo SD rat model, Gal-3 expression level was up-regulated. The inhibition of Gal-3 attenuated the neuroinflammation in SCI model. The inhibition of Gal-3 could also mitigate the neuroinflammation and reactive oxygen species (ROS) in in vitro model. ROS reduced the effect of Gal-3 on oxidative stress in in vitro model. Down-regulating the content of TXNIP decreased the effect of Gal-3 on neuroinflammation in in vitro model. Suppressing the level of NLRP3 could weaken the effect of Gal-3 on neuroinflammation in in vitro model. Our data highlight that the Gal-3 plays a vital role in regulating the severity of neuroinflammation of SCI by enhancing the activation of ROS/TXNIP/NLRP3 signaling pathway. In addition, inflammasome/IL-1ß production probably acts as the therapeutic target in SCI.

16.
J Ultrasound Med ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31721248

RESUMO

OBJECTIVES: The purpose of this study was to develop a patient-specific 3-dimensional model for high-intensity focused ultrasound (HIFU) treatment through the rib cage using patient data. METHODS: Experimental testing to derive parameters used in defining the amount of energy and alteration needed in treatment protocols for upper abdominal disorders under the rib cage was performed. Reconstructed rib cage models based on patient data, tissue-mimicking material phantoms, and magnetic resonance imaging-guided HIFU using a multielement phased array transducer were used in the experiments. Changes in the focal temperature, acoustic power, and acoustic pressure distribution were investigated with and without the presence of the rib cage model. An ExAblate system (InSightec Ltd, Tirat Carmel, Israel) was used to sonicate phantoms by varying the target phantom or rib cage model location. RESULTS: The effect of the rib cage on the acoustic pressure distribution and acoustic power was closely related to the anatomic structures of the ribs. Thermometry revealed that heating at the focus could be controlled by changing either the power or duration of HIFU application to improve the focal temperature change. The focal temperature change was found to be related to the distance between the rib cage model and focus and the shadow area on the transducer elements covered by the rib cage model in the beam path. CONCLUSIONS: Experimental results suggest that the rib cage model is a valuable and useful tool that can provide realistic human anatomic structures and properties for evaluating the effects of the rib cage on ultrasound propagation.

17.
Onco Targets Ther ; 12: 6515-6523, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31616161

RESUMO

Purpose: The efficacy of traditional therapies for oral carcinoma (OC) is limited. Oncolytic adenovirus, a novel strategy of cancer therapy, shows potential use in OC treatment. However, its clinical application is limited by pre-existing neutralizing antibodies. Thus, this study aimed to examine the efficacy of a new modified adenovirus against OC in vitro and in vivo. Materials and methods: A multiple modified adenovirus (MMAD) armed with IL-13 (MMAD-IL-13) was constructed, and its effect on Cal-27 cells was examined. The potency of MMAD-IL-13 was examined in vitro and in vivo. For in vitro experiment, CCK-8 kit was used to determine the IC50 of MMAD-IL-3 in OC cell lines. For in vivo experiment, Cal-27 xenograft models were used to determine the antitumor effect of MMAD-IL-13. Apoptosis was measured in Cal-27 cells by Western blotting assay. Immunity response was detected in Cal-27 xenograft models 7 days after intratumoral injection with MMAD-IL-13. The potency of MMAD and MMAD-IL-13 was compared in Cal-27 peripheral blood mononuclear cells (PBMCs) models. Results: MMAD-IL-13 was successfully constructed; the harvested virus could be replicated and they overexpressed human IL-13 in Cal-27 cells. Compared with MMAD, MMAD-IL-13 showed enhanced antitumor effect in vitro by inducing apoptosis and reducing percentage of M2 macrophages in tumor environment in vivo. MMAD-IL-13 also showed potent antitumor effect in Cal-27, SCC-4, and Tca8113 cells in vitro and in Cal-27 xenograft models in vivo. However, MMAD-IL13 did not harm normal human oral epithelial cells in vitro and exhibited no effect on body weight in Cal-27 xenograft models. In Cal-27 PBMC models, MMAD-IL-13 showed stronger antitumor effect than MMAD. Conclusion: A new oncolytic adenovirus carrying the human IL-13 gene was constructed. This virus effectively led to remission of tumor development and death of OC cells in vivo and in vitro, showing its potential as a clinical cancer therapy.

18.
J Atten Disord ; : 1087054719883031, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31640493

RESUMO

Objective: Whether the abnormal connectome of brain's rich-club structure in adults with attention-deficit hyperactivity disorder (ADHD) remains unclear. Method: The current study used diffusion tensor imaging (DTI) and resting-state functional magnetic resonance imaging (fMRI) to compare the performance of 42 adults with ADHD and 59 typical development (TD) adults. Results: A reduced density of rich-clubs among structural hub nodes, including the bilateral precuneus, the insula, the caudate nucleus, the left putamen, and the right calcarine, was found in adults with ADHD. Moreover, lower global efficiency was found in adults with ADHD than in TD, which might be caused by a reduced density of rich-club connections in ADHD patients. Conclusion: Given that adults with ADHD have greater coupling strength between structural and functional connectivity than TD adults, connectome abnormalities with a reduced rich-club connectivity density might be accompanied by altered functional brain dynamics in ADHD patients.

19.
FEBS Open Bio ; 9(12): 2080-2092, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31642613

RESUMO

Cervical squamous cell carcinoma (CSCC) accounts for a significant proportion of cervical cancer; thus, there is a need for novel and noninvasive diagnostic biomarkers for this malignancy. In this study, we performed integrated analysis of a dataset from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) and differentially expressed miRNAs (DEmiRNAs) between CSCC, cervical intraepithelial neoplasia (CIN) and healthy control subjects. We further established protein-protein interaction and DEmiRNA-target gene interaction networks, and performed functional annotation of the target genes of DEmiRNAs. In total, we identified 1375 DEGs and 19 DEmiRNAs in CIN versus normal control, and 2235 DEGs and 33 DEmiRNAs in CSCC versus CIN by integrated analysis. Our protein-protein interaction network indicates that the common DEGs, Cyclin B/cyclin-dependent kinase 1 (CDK1), CCND1, ESR1 and Aurora kinase A (AURKA), are the top four hub genes. P53 and prostate cancer were identified as significantly enriched signaling pathways of common DEGs and DEmiRNA targets, respectively. We validated that expression levels of three DEGs (TYMS, SASH1 and CDK1) and one DEmiRNA of hsa-miR-99a were altered in blood samples of patients with CSCC. In conclusion, a total of four DEGs (TYMS, SASH1, CDK1 and AURKA) and two DEmiRNAs (hsa-miR-21 and hsa-miR-99a) may be involved in the pathogenesis of CIN and the progression of CIN into CSCC. Of these, TYMS is predicted to be regulated by hsa-miR-99a and SASH1 to be regulated by hsa-miR-21.

20.
Microcirculation ; : e12598, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31660674

RESUMO

OBJECTIVE: Arteriogenesis is an important mechanism that contributes to restoration of oxygen supply in chronically ischemic tissues, but remains incompletely understood due to technical limitations. This study presents a novel approach for comprehensive assessment of the remodeling pattern in a complex microvascular network containing multiple collateral microvessels. METHODS: We have developed a hardware-software integrated platform for quantitative, longitudinal, and label-free imaging of network-wide hemodynamic changes and arteriogenesis at the single-vessel level. By ligating feeding arteries in the mouse ear, we induced network-wide hemodynamic redistribution and localized arteriogenesis. The utility of this technology was demonstrated by studying the influence of obesity on microvascular arteriogenesis. RESULTS: Simultaneously monitoring the remodeling of competing collateral arterioles revealed a new, inverse relationship between initial vascular resistance and extent of arteriogenesis. Obese mice exhibited similar remodeling responses to lean mice through the first week, including diameter increase and flow upregulation in collateral arterioles. However, these gains were subsequently lost in obese mice. CONCLUSIONS: Capable of label-free, comprehensive, and dynamic quantification of structural and functional changes in the microvascular network in vivo, this platform opens up new opportunities to study the mechanisms of microvascular arteriogenesis, its implications in diseases, and approaches to pharmacologically rectify microvascular dysfunction.

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