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Int J Mol Sci ; 21(21)2020 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-33171761


The morphological clues of scaffolds can determine cell behavior and, therefore, the patterning of electroactive polymers can be a suitable strategy for bone tissue engineering. In this way, this work reports on the influence of poly(vinylidene fluoride-co-trifluoroethylene) (P(VDF-TrFE)) electroactive micropatterned scaffolds on the proliferation and differentiation of bone cells. For that, micropatterned P(VDF-TrFE) scaffolds were produced by lithography in the form of arrays of lines and hexagons and then tested for cell proliferation and differentiation of pre-osteoblast cell line. Results show that more anisotropic surface microstructures promote bone differentiation without the need of further biochemical stimulation. Thus, the combination of specific patterns with the inherent electroactivity of materials provides a promising platform for bone regeneration.

Braz J Phys Ther ; 2020 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-32561136


BACKGROUND: Adherence to the use of recommended measures/criteria for return to sport clearance after anterior cruciate ligament reconstruction is crucial for successful rehabilitation. OBJECTIVES: The purpose of this study was to describe the current clinical practice of Brazilian physical therapists that treat patients after anterior cruciate ligament reconstruction , including the measures/criteria used to support the decision-making process regarding return to sport. The secondary aim was to investigate factors associated with the use of the most recommended measures/criteria for RTS. METHODS: An electronic survey questionnaire was sent to Brazilian physical therapists. The survey consisted of questions about demographics and professional and clinical practice data related to anteior cruciate ligament reconstruction postoperative rehabilitation and return to sport criteria. Descriptive statistics and chi-square tests were used for analyses. RESULTS: A sample of 439 professionals participated in the survey. Only 6.4% of the physical therapists use the most recommended measures/criteria for return to sport after anterior cruciate ligament reconstructionACLR. Professional certification in Sports Physical Therapy was the only factor associated with the use of these recommended measures/criteria (p=0.02). The measures most used for return to sport clearance were related to physical factors (65.3% to 75.1%), such as range of motion and muscle strength. A small number of professionals use questionnaires to assess functional (16.6%) and psychological (19.1%) aspects of their patients to support the decision-making process. CONCLUSION: In their clinical practice, most Brazilian physical therapists do not use the recommended measures/criteria for return to sport after anterior cruciate ligament reconstruction.

ACS Appl Mater Interfaces ; 12(1): 60-69, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31808332


Novel microfluidic substrates based on electrospun poly(l-lactic acid) (PLLA) membranes were developed to increase the limited range of commercially available paper substrates, commonly used for the fabrication of microfluidic paper-based analytical devices. PLLA's advantageous properties include biodegradability, biocompatibility, ease of being processed in various tailored morphologies, and cost effectiveness, among others. Oriented and nonoriented electrospun PLLA membranes were fabricated using electrospinning and the influence of fiber orientation, addition of hydrophilic additives, and plasma treatments on the morphology, physicochemical properties, and capillary flow rates were evaluated and compared with the commercial Whatman paper. In addition, a proof-of-concept application based on the colorimetric detection of glucose in printed PLLA and paper-based microfluidic systems was also performed. The results show the potential of PLLA substrates for the fabrication of portable, disposable, eco-friendly, and cost-effective microfluidic systems with controllable properties that can be tailored according to specific biotechnological application requirements, being a suitable alternative to conventional paper-based substrates.

Nursing (Säo Paulo) ; 22(258): 3356-3361, nov.2019.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1053416


Objetivo: Analisar a percepção e os dilemas éticos da equipe de enfermagem frente à decisão de não ressuscitação cardiopulmonar. Método: Estudo descritivo-exploratório, com abordagem qualitativa. Amostra envolve critério por conveniência. Foram entrevistados 18 profissionais de uma unidade de terapia intensiva adulto. Os relatos foram analisados pelo método do Discurso do Sujeito Coletivo. Resultados: A equipe se vê preparada para a decisão de não reanimar, mas desconhecem a existência de protocolos institucionais. A decisão de não reanimação é realizada de forma verbal, o que gera ansiedade e frustração nos profissionais. A família não é informada sobre a decisão da não reanimação. Considerações finais: Tornou-se evidente que os dilemas éticos estão presentes na assistência a esse tipo de situação limítrofe, e que faltam protocolos, normas e envolvimento multiprofissional, para que ocorra uma assistência segura e efetiva tanto para o profissional de saúde quanto para o paciente e sua família.(AU)

Objective: Analyze the perception and ethical dilemmas of the nursing team regarding the decision not to use cardiopulmonary resuscitation. Method: A descriptive-exploratory study with a qualitative approach. Sample involves criterion for convenience. We interviewed 18 professionals from an adult intensive care unit. The reports were analyzed using the Collective Subject Discourse method. Results: The team is prepared for the decision not to reanimate, but they are unaware of the existence of institutional protocols. The decision not to resuscitate is performed verbally, which creates anxiety and frustration in professionals. The family is not informed of the decision not to reanimate. Final considerations: It has become clear that ethical dilemmas are present in the assistance to this type of borderline situation, and that protocols, norms and multiprofessional involvement are lacking, so that a safe and effective assistance can occur both for the health professional and for the patient and your family.(AU)

Objetivo: Analizar la percepción y los dilemas éticos del equipo de enfermería con respecto a la decisión de no utilizar la reanimación cardiopulmonar. Método: Un estudio descriptivo-exploratorio con un enfoque cualitativo. Muestra implica criterio de conveniencia. Entrevistamos a 18 profesionales de una unidad de cuidados intensivos para adultos. Los informes fueron analizados utilizando el método de Discurso de sujeto colectivo. Resultados: el equipo está preparado para la decisión de no reanimar, pero desconocen la existencia de protocolos institucionales. La decisión de no reanimar se realiza verbalmente, lo que genera ansiedad y frustración en los profesionales. La familia no está informada de la decisión de no reanimar. Consideraciones finales: Ha quedado claro que existen dilemas éticos en la asistencia a este tipo de situación límite, y que faltan protocolos, normas y participación multiprofesional, de modo que pueda darse una asistencia segura y efectiva tanto para el profesional de la salud como para el paciente. Y su familia.(AU)

Humanos , Bioética , Reanimação Cardiopulmonar , Ética em Enfermagem , Unidades de Terapia Intensiva , Equipe de Enfermagem
BMC Public Health ; 19(1): 581, 2019 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-31096940


BACKGROUND: The inadequate management of solid waste impacts populations' health and quality of life, and disproportionately affects developing countries. This study aims to describe a protocol for epidemiological diagnosis, the purpose being to estimate the prevalence of chronic and communicable and non communicable diseases in waste pickers, and the occupational and environmental risk factors to which these are exposed. METHODS: This is a cross-sectional study, based on survey design in an area of extreme social vulnerability - the largest garbage dump in Latin America. Using a multidimensional research protocol, divided in three stages: 1- The identification of the subjects, and the scheduling of tests; 2- Situational diagnosis through interviews, anthropometric evaluation, measuring blood pressure, collecting hair and nail samples to detect exposure to heavy metals and undertaking laboratory tests; 3- The return of the waste pickers to receive the test results, followed by referral to the health team and to report occupational accidents. RESULTS: One thousand twenty-five waste pickers undertook tests and interviews. The majority were women (67.0%), with 36-45 years old (45.7%), and 96.0% had children. In total, 27.3% of the participants did not attend to any school and 47.7% were educated only up to primary level. The majority of waste pickers (68.70%) reported accidents and most of them (89.69%) were related to sharp objects. The mean time working in this open dump was 15 years. According the anthropometric measure, 32.6% were overweight and 21.1% were obese. The most common reported diseases were: osteomuscular disorders (78.7%); arboviruses (28.6%); episodic diarrhea (24.9%); hypertension (24.2%); bronchitis (14.3%); intestinal worms (12.6%) and diabetes (10.1%). According to the blood tests, the values outside the reference limits were: Uric acid (23.89%); creatinine (54.06%); GGT range (16.04%); SGOT - Serum Glutamic Oxaloacetic Transaminase (5.29%); SGPT - serum Glutamic-Pyruvic Transaminase (35.52%). CONCLUSIONS: This study is the first to evaluate multiple risks and diseases in the majority of waste pickers working in the largest garbage dump of a continent. These findings highlight the importance to address urgently the environmental, social and health impacts related to the management of solid waste in developmental countries to protect these workers and their families.

Acidentes de Trabalho/estatística & dados numéricos , Resíduos de Alimentos , Doenças Profissionais/epidemiologia , Resíduos Sólidos , Adulto , Pré-Escolar , Estudos Transversais , Países em Desenvolvimento , Projetos de Pesquisa Epidemiológica , Feminino , Humanos , América Latina/epidemiologia , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/etiologia , Qualidade de Vida , Medição de Risco
Rev. CES psicol ; 12(1): 54-68, ene.-abr. 2019.
Artigo em Português | LILACS-Express | LILACS | ID: biblio-1057139


Resumo O presente artigo tem como objetivo relatar sobre a organização neurológica das crianças em relação ao comportamento motor, buscando evidenciar os processos anteriores ao uso preferencial de um determinado membro e a constituição da lateralidade, salientando as leis céfalo-caudal e próximo-distal. Também são apresentados conceitos referentes ao desenvolvimento motor em neonatos e de que forma irão influenciar a relação de lateralidade discutida. Objetiva-se apresentar a relação entre a lateralidade e a discriminação direita-esquerda e como esta última influencia na aprendizagem. O estudo se deu a partir de revisões teóricas, e os resultados obtidos mostram que o desenvolvimento motor, partindo de neonatos, se dá primeiro com uma constituição bimanual, havendo uma dominância homolateral do corpo, devido à hipotonicidade do eixo axial e à hipertonicidade das extremidades. Uma melhor estruturação do esquema corporal ocorre a partir do desaparecimento dos reflexos presentes e da constituição da bonicularidade e da binauricularidade (uso simultâneo dos olhos e dos ouvidos, respectivamente), os quais favorecem o engatinhar e, consequentemente, a criança passa a ter maiores possibilidades de explorar o mundo a sua volta, favorecendo a definição de preferência de um dos lados. Conforme apresentado, se a discriminação direita-esquerda não for bem definida, poderão ocorrer problemas cognitivos e distúrbios de aprendizagem.

Abstract This paper aims to discuss about the neurological organization of children in relation to motor behavior, seeking to highlight the prior processes to the preferential use of a member and laterality constitution, emphasizing the cephalocaudal and proximodistal trends. It also provides concepts regarding to motor development in newborns and how it will influence the relationship of laterality. It aims to show the relationship between handedness and right-left differentiation and how the latter influence on learning. This study was based on theoretical revisions, and the results obtained show that the motor development, starting from newborns, occurs first with a bimanual constitution, with a homolateral dominance of the body due to the hypotonicity of the axial axis and hypertonicity of the extremities A better structure of the body schema occurs from the disappearance of existing reflexes and the constitution of bonicularidade and binauricularidade (simultaneous use of eyes and ears, respectively), which favors crawling and, consequently, the child will have greater possibilities to explore the surrounding world, favoring the definition of preference on one side. As shown, if the right-left differentiation is not well defined, cognitive problems and learning disorders may occur.

Resumen El presente artículo tiene como objetivo discutir sobre la organización neurológica de los niños en relación al comportamiento motor, buscando evidenciar los procesos previos al uso preferencial de un determinado miembro y la constitución de la lateralidad, enfatizando las leyes céfalo-caudal y próximo-distal. También se presentan conceptos referentes al desarrollo motor en recién nacidos y el modo como influenciará la relación de lateralidad discutida; y la relación entre la lateralidad y la discriminación derecha-izquierda y cómo esta última influye en el aprendizaje. El estudio se basó en revisiones teóricas y los resultados obtenidos muestran que el desarrollo motor, partiendo de los recién nacidos, se produce primero con una constituición bimanual y una dominancia homolateral del cuerpo, debida a la hipotonicidad del eje axial y a la hipertonicidad de las extremidades. Una mejor estructuración del esquema corporal ocurre a partir de la desaparición de los reflejos presentes y de la constituición de la bonicularidad y de la binauricularidad (uso simultâneo de los ojos y oidos, respectivamente), los cuales favorecen el gatear y, en consecuencia, el niño tendrá mayores posibilidades de explorar el mundo a su alrededor, propiciando la definición de la preferencia de uno de los lados. Como se muestra, si la discriminación derecha-izquierda no está bien definida, pueden ocurrir problemas cognitivos y trastornos en el aprendizaje.

Texto & contexto enferm ; 28: e20170279, 2019. tab, graf
Artigo em Inglês | LILACS, BDENF - Enfermagem | ID: biblio-1004807


ABSTRACT Objective: to describe some methodological considerations on the Systematic Review of Mixed Methods developed by the Joanna Briggs Institute, seeking to disseminate the use of this methodology, exemplifying its use and demonstrating the benefits for nursing research. Method: it is an article of reflection. Result: it can be seen that the Systematic Review of Mixed Methods demonstrates that it is a research method that aligns with the characteristics of nursing care, making it possible to make a broader analysis of the different aspects of care such as: the viability of an activity or intervention, its adequacy to a specific context, the significance of the intervention for the patient and their effectiveness. Conclusion: the possibility of incorporating scientific evidence into the clinical practice by the decision making, increasing the focus of care and bringing benefits to the patients as well as the public policies is envisaged. This method values the context of care, cost-effectiveness and the preferences of patients, caregivers and health care providers.

RESUMEN Objetivo: describir algunas consideraciones metodológicas sobre la Revisión Sistemática de Métodos Mixtos desarrollada por el Instituto Joanna Briggs, buscando divulgar el uso de esta metodología, ejemplificando su utilización y demostrando los beneficios para la investigación en enfermería. Método: se trata de un artículo de reflexión. Resultados: se observa que la Revisión Sistemática de Métodos Mixtos demuestra ser un método de investigación que se alinea a las características del cuidado en enfermería, posibilitando hacer un análisis más amplio de los diferentes aspectos del cuidado como: la viabilidad de una evaluación actividad o intervención, su adecuación a un determinado contexto, la significancia de la intervención para el paciente y su eficacia. Conclusión: se vislumbra la posibilidad de incorporar evidencias científicas a la práctica clínica por la toma de decisión, ampliando el foco de cuidado y aportando beneficios a los pacientes y a las políticas públicas. Este método valora el contexto de la atención, la relación costo-eficacia, así como las preferencias de los pacientes, cuidadores y proveedores de atención de salud.

RESUMO Objetivo: descrever algumas considerações metodológicas sobre a Revisão Sistemática de Métodos Mistos desenvolvida pelo Instituto Joanna Briggs, buscando divulgar o uso desta metodologia, exemplificando sua utilização e demonstrando os benefícios para a pesquisa em enfermagem. Método: trata-se de um artigo de reflexão. Resultado: percebe-se que a Revisão Sistemática de Métodos Mistos demonstra ser um método de pesquisa que se alinha às características do cuidado em enfermagem, possibilitando fazer uma análise mais ampla dos diferentes aspectos do cuidado como: a viabilidade de uma atividade ou intervenção, sua adequação a um determinado contexto, a significância da intervenção para o paciente e a sua eficácia. Conclusão: vislumbra-se a possibilidade de incorporar evidências científicas à prática clínica pela tomada de decisão, ampliando o foco de cuidado e trazendo benefícios aos pacientes bem como às políticas públicas. Este método valoriza o contexto da prestação de cuidados, a relação custo-eficácia e as preferências dos pacientes, cuidadores e provedores de cuidados de saúde.

Humanos , Enfermagem , Técnicas , Metodologia , Prática Clínica Baseada em Evidências , Revisão Sistemática
Codas ; 30(5): e20170225, 2018 Oct 22.
Artigo em Português, Inglês | MEDLINE | ID: mdl-30365649


PURPOSE: Characterize voices of adult individuals without vocal complaints and verify the effect of gender and age with the use of acoustic measures. METHODS: One-hundred and seventy-six voice recordings belonging to adults between 19 and 59 years old, divided into four age groups, for decade, recorded in a database were analyzed. All voices analyzed were classified with no deviation in vocal quality. Acoustic analysis of the parameters was performed: Fundamental Frequency (sustained vowel and connected speech), Jitter, Shimmer and Noise-to-Harmonic Ratio through Multi Dimension Voice Program (KayPentax) software. The effect of gender, age and possible interactions were verified through the Factorial Anova test. When necessary, post hoc was performed with the Least Significant Difference test. RESULTS: There were changes in the voice as a function of age, with a decrease in the Fundamental Frequency in the vowel and connected speech modalities in women and in the Fundamental Frequency of the speech in men. In men, an increase in shimmer measure was observed with the advancing age. Differences between genders were found in the measures of Fundamental Frequency, Jitter and Noise to Harmonic Ratio. CONCLUSION: Vocal changes due to advancing age can be identified acoustically at the end of adulthood, and in women, these changes can be marked previously to the menopausal period.

Acústica da Fala , Qualidade da Voz/fisiologia , Adulto , Fatores Etários , Envelhecimento/fisiologia , Análise de Variância , Estudos Transversais , Feminino , Humanos , Masculino , Menopausa/fisiologia , Pessoa de Meia-Idade , Valores de Referência , Fatores Sexuais , Medida da Produção da Fala/métodos , Adulto Jovem
Folia Phoniatr Logop ; 70(2): 82-89, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30041244


OBJECTIVES: This study establishes normative nasalance values for middle-aged and elderly Brazilian Portuguese-speakers and investigates age and gender effects across the life span. METHODS: Nasalance scores were obtained from 62 middle-aged (45-59 years) and 60 elderly (60-79 years) participants with normal speech for 3 nonnasal, 1 phonetically balanced, and 2 nasal-loaded test sentences using the Nasometer II 6400. The data were combined with a published data set of 237 speakers in 4 groups: children (5-9 years), adolescents (10-19 years), young adults (20-24 years), and mature adults (25-35 years). A repeated-measures analysis of variance was used to investigate differences between the stimuli by gender and age groups. RESULTS: There were statistically significant effects of stimulus, gender, and age group, as well as a stimulus-age group interaction effect and a gender-age group interaction effect. The females' mean nasalance scores were higher than those of the males. The mean nasalance scores for the child, adolescent, and young and mature adult speakers were significantly lower than those for the elderly speakers, and the children's scores were significantly lower than those of the middle-aged speakers. CONCLUSION: Higher nasalance scores among middle-aged and elderly speakers may indicate physiological changes affecting oral-nasal balance in speech across the life span.

Qualidade da Voz/fisiologia , Idoso , Envelhecimento/fisiologia , Brasil , Conjuntos de Dados como Assunto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Boca , Cavidade Nasal , Caracteres Sexuais , Acústica da Fala , Medida da Produção da Fala
BMC Nephrol ; 19(1): 179, 2018 07 13.
Artigo em Inglês | MEDLINE | ID: mdl-30005635


BACKGROUND: Angiotensin II (Ang II) contributes to the progression of renal diseases associated with proteinuria and glomerulosclerosis mainly by inducing podocyte apoptosis. In the present study, we investigated whether the chronic effects of Ang II via AT1 receptor (AT1R) would result in endoplasmic reticulum (ER) stress/PKC-delta/p38 MAPK stimulation, and consequently podocyte apoptosis. METHODS: Wistar rats were treated with Ang II (200 ng·kg-1·min-1, 42 days) and or losartan (10 mg·kg-1·day-1, 14 days). Immortalized mouse podocyte were treated with 1 µM Ang II and/or losartan (1 µM) or SB203580 (0.1 µM) (AT1 receptor antagonist and p38 MAPK inhibitor) for 24 h. Kidney sections and cultured podocytes were used to evaluate protein expression by immunofluorescence and immunoblotting. Apoptosis was evaluated by flow cytometry and intracellular pH (pHi) was analyzed using microscopy combined with the fluorescent probe BCECF/AM. RESULTS: Compared with controls, Ang II via AT1R increased chaperone GRP 78/Bip protein expression in rat glomeruli (p < 0.001) as well as in podocyte culture (p < 0.01); increased phosphorylated eIf2-α (p < 0.05), PKC-delta (p < 0.01) and p38 MAPK (p < 0.001) protein expression. Furthermore, Ang II induced p38 MAPK-mediated late apoptosis and increased the Bax/Bcl-2 ratio (p < 0.001). Simultaneously, Ang II via AT1R induced p38 MAPK-NHE1-mediated increase of pHi recovery rate after acid loading. CONCLUSION: Together, our results indicate that Ang II-induced podocyte apoptosis is associated with AT1R/ER stress/PKC-delta/p38 MAPK axis and enhanced NHE1-mediated pHi recovery rate.

Angiotensina II/toxicidade , Estresse do Retículo Endoplasmático/fisiologia , Podócitos/metabolismo , Proteína Quinase C-delta/metabolismo , Trocador 1 de Sódio-Hidrogênio/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Animais , Apoptose/efeitos dos fármacos , Apoptose/fisiologia , Linhagem Celular Transformada , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Ativação Enzimática/efeitos dos fármacos , Ativação Enzimática/fisiologia , Camundongos , Podócitos/efeitos dos fármacos , Isoformas de Proteínas/metabolismo , Distribuição Aleatória , Ratos , Ratos Wistar
Rev. pesqui. cuid. fundam. (Online) ; 10(3): 856-862, jul.-set. 2018.
Artigo em Inglês, Português | LILACS, BDENF - Enfermagem | ID: biblio-906762


Objective: The study's goal has been to analyze how the pregnant woman perceives the partner's involvement in the prenatal routine. Methods: It is a descriptive-exploratory study with a qualitative approach. Data collection was performed from March to June 2016, through a semi-structured interview. The research participants were 11 pregnant women, starting at the 28th week of gestation, who were submitted to content analysis in the thematic modality. Results: Data analysis shows that only one participant had complete follow-up by the partner, another five reported the partner's presence only during the obstetrical ultrasonography procedure. Regarding the women that reported partner's absence, the work was highlighted as the main factor; it was also observed that gender issues influence this absence, since the pregnancy is seen as a woman exclusive moment. Conclusion: It is essential that health services can become sensitive to the insertion of the partner in this routine. Furthermore, an intersectoral partnership between the health, the legal and the educational sectors must become a reality in order to stimulate this change

Objetivo: Analisar como a gestante percebe a participação do parceiro na rotina pré-natal. Método: Estudo descritivo exploratório de abordagem qualitativa. Coleta de dados entre março e junho de 2016, por meio de entrevista semiestruturada, com 11 gestantes, a partir da 28ª semana de gestação, sendo estes submetidos à análise de conteúdo na modalidade temática. Resultados: Das participantes do estudo, apenas uma teve acompanhamento integral do parceiro, outras cinco relataram a presença apenas na realização da ultrassonografia obstétrica. Daquelas que relataram ausência, o trabalho foi apontado como principal fator, também se observou que questões de gênero influenciam nesta ausência, pois a gestação é vista como momento exclusivo da mulher. Conclusão: É imprescindível que os serviços de saúde estejam sensíveis à inserção do parceiro nesta rotina, e que haja parcerias intersetoriais entre a saúde, o setor jurídico e a educação que possam estimular esta mudança

Objetivo: Analizar cómo la madre percibe la participación del socio en la rutina prenatal. Método: Estudio exploratorio descriptivo de enfoque cualitativo. Recolección se llevó a cabo entre marzo y junio el año 2016, através de entrevistas semiestructuradas con 11 mujeres embarazadas desde la semana 28 de embarazo, que están sometidas a análisis de contenido en la modalidad temática. Resultados: De las participantes en el estudio, sólo uno tenía pareja de supervisión, otras cinco personas informaron de la presencia sólo en la realización de ultrasonido obstétrico. De los que reportaron ausencia, el trabajo fue considerado como el principal factor, también observaron que las cuestiones de género influyen en esta ausencia porque el embarazo es visto como un momento único de la mujer. Conclusión: Es esencial que los servicios de salud son sensibles a la inclusión del socio en esta rutina, y que tienen alianzas intersectoriales entre la salud, el sector jurídico y la educación que pueden estimular este cambio

Humanos , Masculino , Feminino , Gravidez , Adulto , Gestantes , Cuidado Pré-Natal , Cônjuges , Paternidade , Saúde da Mulher
Nat Protoc ; 13(4): 681-704, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29543796


Poly(vinylidene fluoride) (PVDF) and its copolymers are the polymers with the highest dielectric constants and electroactive responses, including piezoelectric, pyroelectric and ferroelectric effects. This semicrystalline polymer can crystallize in five different forms, each related to a different chain conformation. Of these different phases, the ß phase is the one with the highest dipolar moment and the highest piezoelectric response; therefore, it is the most interesting for a diverse range of applications. Thus, a variety of processing methods have been developed to induce the formation of the polymer ß phase. In addition, PVDF has the advantage of being easily processable, flexible and low-cost. In this protocol, we present a number of reproducible and effective methods to produce ß-PVDF-based morphologies/structures in the form of dense films, porous films, 3D scaffolds, patterned structures, fibers and spheres. These structures can be fabricated by different processing techniques, including doctor blade, spin coating, printing technologies, non-solvent-induced phase separation (NIPS), temperature-induced phase separation (TIPS), solvent-casting particulate leaching, solvent-casting using a 3D nylon template, freeze extraction with a 3D poly(vinyl alcohol) (PVA) template, replica molding, and electrospinning or electrospray, with the fabrication method depending on the desired characteristics of the structure. The developed electroactive structures have shown potential to be used in a wide range of applications, including the formation of sensors and actuators, in biomedicine, for energy generation and storage, and as filtration membranes.

Fenômenos Eletromagnéticos , Microtecnologia/métodos , Polivinil/química
Polymers (Basel) ; 10(2)2018 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-30966197


Fluorinated polymers constitute a unique class of materials that exhibit a combination of suitable properties for a wide range of applications, which mainly arise from their outstanding chemical resistance, thermal stability, low friction coefficients and electrical properties. Furthermore, those presenting stimuli-responsive properties have found widespread industrial and commercial applications, based on their ability to change in a controlled fashion one or more of their physicochemical properties, in response to single or multiple external stimuli such as light, temperature, electrical and magnetic fields, pH and/or biological signals. In particular, some fluorinated polymers have been intensively investigated and applied due to their piezoelectric, pyroelectric and ferroelectric properties in biomedical applications including controlled drug delivery systems, tissue engineering, microfluidic and artificial muscle actuators, among others. This review summarizes the main characteristics, microstructures and biomedical applications of electroactive fluorinated polymers.

Adv Healthc Mater ; 7(5)2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29280314


Magnetic nanoparticles (NPs) are emerging as an important class of biomedical functional nanomaterials in areas such as hyperthermia, drug release, tissue engineering, theranostic, and lab-on-a-chip, due to their exclusive chemical and physical properties. Although some works can be found reviewing the main application of magnetic NPs in the area of biomedical engineering, recent and intense progress on magnetic nanoparticle research, from synthesis to surface functionalization strategies, demands for a work that includes, summarizes, and debates current directions and ongoing advancements in this research field. Thus, the present work addresses the structure, synthesis, properties, and the incorporation of magnetic NPs in nanocomposites, highlighting the most relevant effects of the synthesis on the magnetic and structural properties of the magnetic NPs and how these effects limit their utilization in the biomedical area. Furthermore, this review next focuses on the application of magnetic NPs on the biomedical field. Finally, a discussion of the main challenges and an outlook of the future developments in the use of magnetic NPs for advanced biomedical applications are critically provided.

Dispositivos Lab-On-A-Chip , Nanopartículas de Magnetita , Nanocompostos , Nanomedicina Teranóstica/métodos , Humanos , Nanopartículas de Magnetita/química , Nanopartículas de Magnetita/uso terapêutico , Nanocompostos/química , Nanocompostos/uso terapêutico , Nanomedicina Teranóstica/instrumentação
CoDAS ; 30(5): e20170225, 2018. tab
Artigo em Português | LILACS-Express | LILACS | ID: biblio-984220


RESUMO Objetivo Caracterizar as vozes de indivíduos adultos sem queixas vocais e verificar o efeito de gênero e idade a partir de um conjunto de medidas acústicas. Método Foram analisadas 176 gravações de vozes pertencentes a adultos com idades entre 19 e 59 anos, divididas em quatro grupos etários, por década, armazenadas em uma base de dados. Todas as vozes analisadas foram classificadas com ausência de desvio na qualidade vocal. Realizou-se análise acústica dos parâmetros: Frequência Fundamental (vogal sustentada e fala encadeada), Jitter , Shimmer e Noise-to-Harmonic Ratio por meio software Multi Dimension Voice Program (KayPentax). O efeito de gênero, idade e possíveis interações foram verificados por meio do teste Anova Fatorial. Quando necessário, realizou-se post hoc com o teste Least Significant Difference. Resultados Houve mudanças na voz em função da idade, com diminuição da Frequência Fundamental nas modalidades vogal e fala encadeada em mulheres e na Frequência Fundamental da fala em homens. Em homens, foi observado aumento da medida de shimmer com o avanço da idade. Foram verificadas diferenças entre os gêneros nas medidas de Frequência Fundamental, Jitte r e Noise to Harmonic Ratio. Conclusão Mudanças vocais decorrentes do avanço da idade podem ser identificadas acusticamente, no final da fase adulta e, em mulheres, essas mudanças podem ser marcadas previamente ao período da menopausa.

ABSTRACT Purpose Characterize voices of adult individuals without vocal complaints and verify the effect of gender and age with the use of acoustic measures. Methods One-hundred and seventy-six voice recordings belonging to adults between 19 and 59 years old, divided into four age groups, for decade, recorded in a database were analyzed. All voices analyzed were classified with no deviation in vocal quality. Acoustic analysis of the parameters was performed: Fundamental Frequency (sustained vowel and connected speech), Jitter, Shimmer and Noise-to-Harmonic Ratio through Multi Dimension Voice Program (KayPentax) software. The effect of gender, age and possible interactions were verified through the Factorial Anova test. When necessary, post hoc was performed with the Least Significant Difference test. Results There were changes in the voice as a function of age, with a decrease in the Fundamental Frequency in the vowel and connected speech modalities in women and in the Fundamental Frequency of the speech in men. In men, an increase in shimmer measure was observed with the advancing age. Differences between genders were found in the measures of Fundamental Frequency, Jitter and Noise to Harmonic Ratio. Conclusion Vocal changes due to advancing age can be identified acoustically at the end of adulthood, and in women, these changes can be marked previously to the menopausal period.

Kidney Blood Press Res ; 42(6): 1277-1289, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29262407


BACKGROUND/AIMS: To assess the possible contribution of the ß-adrenergic overstimulation in early stages of renal injury, the present study evaluated, in rats, the effects of the ß-adrenoceptor agonist isoproterenol (ISO) on renal function and morphology, as well as the renal mRNA and protein expression of the NADPH oxidase isoform 4 (Nox 4) and subunit p22phox, endoplasmic reticulum (ER) stress, pro-inflammatory, pro-apoptotic and renin-angiotensin system (RAS) components. METHODS: Wistar rats received ISO (0.3 s.c.) or vehicle (control) for eight days. At the end of the treatment, food and water intake, urine output and body weight gain were evaluated and renal function studies were performed. Renal tissue was used for the morphological, quantitative PCR and immunohistochemical studies. RESULTS: ISO did not change metabolic parameters or urine output. However it induced a decrease in renal blood flow and an increase in the filtration fraction. These changes were accompanied by increased cortical mRNA and protein expression for the renal oxidative stress components including Nox 4 and p22phox; ER stress, pro-inflamatory, pro-apoptotic as well as RAS components. ISO also induced a significant increase in medullar renin protein expression. CONCLUSION: These findings support relevant information regarding the contribution of specific ß-adrenergic hyperactivity in early stage of renal injury, indicating the reactive oxygen species, ER stress and intrarenal RAS as important factors in this process.

Agonistas Adrenérgicos beta/farmacologia , Rim/lesões , Animais , Estresse do Retículo Endoplasmático , Isoproterenol/farmacologia , Testes de Função Renal , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio , Sistema Renina-Angiotensina
Codas ; 29(3): e20150311, 2017 Jun 08.
Artigo em Português, Inglês | MEDLINE | ID: mdl-28614457


Purpose: To investigate vocal quality variability after applying tongue trills associated with transcutaneous electrical nerve stimulation (TENS) on the larynx of women with normal laryngeal function. Additionally, to verify the effect of this technique over time on voice quality. Methods: Participants were 40 women (average 23.4 years) without vocal complaints. The procedure involved tongue trills with or without TENS for 3 minutes, rest and repeating the technique for another 2 minutes. The participants' voices were recorded before (Pre), after three minutes (Post 3min) and after two additional minutes (Post 5min) applying the technique. TENS with two electrodes was used on the thyroid cartilage. Self-assessment, acoustic and perceptual analysis were performed. Results: When comparing tongue trills in isolation and associated with TENS, a greater sense of stability in phonation (self-assessment) and improvement in voice quality (perceptual evaluation) was observed in the combination technique. There was no statistical difference in acoustics findings between tongue trills in isolation and associated with TENS. When comparing the time effect of tongue trills with TENS in self-assessment there was a perception of less muscle tension (3min) and greater comfort during phonation (5 min); in the acoustic analysis, there was an increase of F0 (3 and 5 min) and intensity (5 min) when compared to Pre-moment; in the perceptual evaluation, better voice quality (3min). Conclusion: Comparing tongue trills in isolation and associated with TENS, there were changes in the comfort and muscle tension perception, as well as in vocal quality. On the other hand, tongue trills associated with TENS performed in 3 or 5 minutes resulted in beneficial effects on the voice identified in the assessments.

Língua/fisiologia , Estimulação Elétrica Nervosa Transcutânea , Treinamento da Voz , Voz/fisiologia , Adolescente , Adulto , Feminino , Humanos , Fonação/fisiologia , Medida da Produção da Fala , Fatores de Tempo , Qualidade da Voz , Adulto Jovem
Colloids Surf B Biointerfaces ; 157: 48-55, 2017 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-28575740


Zinc-decorated magnetic silica spheres were developed, optimized and tested for the capture and separation of l-histidine. The magnetic silica spheres were prepared using a simple sol-gel method and show excellent magnetic characteristics, adsorption capacity toward metal ions, and stability in aqueous solution in a wide pH range. The binding capacity of zinc-decorated magnetic silica spheres to histidine proved to be strongly influenced by the morphology, composition and concentration of metal at the surface of the magnetic silica spheres and therefore these parameters should be carefully controlled in order to maximize the performance for protein purification purposes. Optimized zinc-decorated magnetic silica spheres demonstrate a binding capacity to l-histidine of approximately 44mgg-1 at the optimum binding pH buffer.

Magnetismo , Proteínas/química , Dióxido de Silício/química , Zinco/química , Adsorção , Biotecnologia , Histidina/química , Concentração de Íons de Hidrogênio
Rev. Pesqui. (Univ. Fed. Estado Rio J., Online) ; 9(2): 340-346, abr.-jun. 2017.
Artigo em Inglês, Português | LILACS, BDENF - Enfermagem | ID: biblio-836348


Objective: To analyze the perception of adolescents about the life activity “express sexuality”. Method: This is an action-research developed at a school in the periphery of Fortaleza, Ceará, Brazil. We selected 25 adolescents to participate in an educational workshop on sexuality. Results: We observed vulnerability of adolescents to early pregnancy and STDs. Despite having prior knowledge about safe sex practices, we verified they expose themselves to risky situations. Conclusion: We concluded that the inclusion of school activities that promote not only the acquisition of knowledge, but, above all, activities of reflection in search of awareness of body protection, prevention of unwanted pregnancy and STDs is needed.

Objetivo: Analisar a percepção de adolescentes acerca da atividade de vida “exprimir sexualidade”. Método: Trata-se de pesquisa-ação desenvolvida em uma escola localizada na periferia de Fortaleza, Ceará. Foram selecionados 25 adolescentes para participarem de uma oficina educativa sobre sexualidade. Resultados: Observou-se vulnerabilidade dos adolescentes para gravidez precoce e DSTs. Verificou-se que, apesar de possuírem conhecimento prévio sobre práticas sexuais seguras, expõem-se a situações de risco. Conclusões: Conclui-se que há necessidade de inserção de atividades na escola que promovam não apenas aquisição de conhecimento, mas, sobretudo atividades de reflexão em buscada conscientização sobre proteção do corpo, prevenção de gravidez indesejada e DSTs.

Objetivo: Analizar la percepción de adolescentes acerca de la actividad de vida “expresar la sexualidad”. Método: Investigación-acción desarrollada en escuela de Fortaleza, Ceará, Brasil. Fueron elegidos 25 adolescentes para participar de taller educativo sobre sexualidad. Resultados: Fue observada vulnerabilidad de adolescentes a embarazos precoces y enfermedades de transmisión sexual. A pesar de tener conocimiento previo acerca de las prácticas de sexo seguro, los adolescentes se exponen a situaciones de riesgo. Conclusiones: Hay necesidad de inclusión de actividades escolares que promueven no sólo la adquisición de conocimientos, pero actividades de reflexión en busca de la conciencia acerca de la protección del cuerpo y la prevención de embarazos no deseados y ETS.

Humanos , Adolescente , Desenvolvimento Psicossexual , Educação em Saúde , Promoção da Saúde , Sexualidade/psicologia , Brasil , Estilo de Vida Saudável