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1.
PLoS One ; 15(1): e0227500, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31923228

RESUMO

A new species of the Campylobacter genus is described, isolated from the preputial mucosa of bulls (Bos taurus). The five isolates obtained exhibit characteristics of Campylobacter, being Gram-negative non-motile straight rods, oxidase positive, catalase negative and microaerophilic. Phenotypic characteristics and nucleotide sequence analysis of 16S rRNA and hsp60 genes demonstrated that these isolates belong to a novel species within the genus Campylobacter. Based on hsp60 gene phylogenetic analysis, the most related species are C. ureolyticus, C. blaseri and C. corcagiensis. The whole genome sequence analysis of isolate FMV-PI01 revealed that the average nucleotide identity with other Campylobacter species was less than 75%, which is far below the cut-off for isolates of the same species. However, whole genome sequence analysis identified coding sequences highly homologous with other Campylobacter spp. These included several virulence factor coding genes related with host cell adhesion and invasion, transporters involved in resistance to antimicrobials, and a type IV secretion system (T4SS), containing virB2-virB11/virD4 genes, highly homologous to the C. fetus subsp. venerealis. The genomic G+C content of isolate FMV-PI01 was 28.3%, which is one of the lowest values reported for species of the genus Campylobacter. For this species the name Campylobacter portucalensis sp. nov. is proposed, with FMV-PI01 (= LMG 31504, = CCUG 73856) as the type strain.

3.
Front Microbiol ; 10: 1657, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31379799

RESUMO

Multidrug-resistant (MDR) Klebsiella pneumoniae is considered a major global concern by the World Health Organization. Evidence is growing on the importance of circulation of MDR bacterial populations between animals and humans. Horses have been shown to carry commensal isolates of this bacterial species and can act as human MDR bacteria reservoirs. In this study, we characterized an extended-spectrum ß-lactamase (ESBL)-producing K. pneumoniae sequence type (ST) 348 isolate from a horse, an ST reported for the first time in an animal, using next-generation sequencing. We compared it with six other MDR K. pneumoniae ST348 human isolates previously identified in health-care facilities in Portugal using a core genome multi-locus sequence typing approach to evaluate a possible genetic link. The horse isolate was resistant to most of the antimicrobials tested, including 3rd generation cephalosporins, fluoroquinolones, and aminoglycosides, and presented several antimicrobial resistance genes, including bla ESBL. Twenty-one allele differences were found between the horse isolate and the most similar human isolate, suggesting a recent common ancestor. Other similarities were observed regarding the content on antimicrobial resistance genes, plasmid incompatibility groups, and capsular and somatic antigens. This study illustrates the relevance of the dissemination of MDR strains, and enhances that identification of these types of bacterial strains in both human and veterinary settings is of significant relevance in order to understand and implement combined control strategies for MDR bacteria in animals and humans.

4.
Rev. bras. farmacogn ; 29(3): 389-399, May-June 2019. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1020573

RESUMO

ABSTRACT Stingless bee products such as honey, pollen, propolis, and geopropolis have been used for centuries in traditional medicine for the treatment of several illnesses. Investigation of the biological activity of stingless bee products, especially propolis and geopropolis, has revealed promising therapeutic properties. About 20% of total Neotropical stingless bees can be found in Brazil. Despite the species diversity, studies on their biological activity are scarce. The present review focuses on the antioxidant and antimicrobial activities of propolis and geopropolis from Brazilian stingless bees. In addition, the toxicity of these natural products was addressed. In order to provide new evidences for the toxic potential of propolis and geopropolis components, an in silico analysis was performed using the ADMET PredictorTM software. We observed that most of studies evaluated only crude ethanol extracts of a limited number of stingless bees species. Propolis and geopropolis displayed antioxidant capacity and antimicrobial activity. Concerning the toxic potential, the extracts of stingless bees propolis and geopropolis were considered safe. Nonetheless, in vitro and in vivo toxicological studies are still necessary.

5.
Clin J Gastroenterol ; 12(6): 583-587, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31111432

RESUMO

We present the case of a woman infected with the HIV type 1, controlled with highly active antiretroviral therapy. In the meantime, she developed a severe perianal disease, with complex fistulae and chronic anal fissures. After developing a severe chronic diarrhea, a total ileocolonoscopy with biopsies was performed, showing multiple ileal and segmental colonic erosions. Histology favoured a Crohn's disease diagnosis. Despite the limited experience of anti-tumour necrosis factor agents in the HIV-infected population, infliximab was started in this patient, due to her severe and symptomatic Crohn's disease, with a controlled HIV infection. No side effects were reported and her bowel movements and perianal disease improved right after induction regimen with infliximab. 1 year after starting this therapy she is in clinical and endoscopic remission. The CD4+ T-cell count remained stable, the HIV-RNA undetectable and no opportunistic infections were reported during follow-up period. Data concerning the use of anti-tumour necrosis factor drugs is limited in patients with both inflammatory bowel disease and HIV infection. Only three cases of Crohn's disease and concomitant HIV infection treated with infliximab were reported in the literature. This case report might help future decisions in patients with a similar clinical situation.

6.
Rev. bras. med. fam. comunidade ; 14(41): 1816, fev. 2019.
Artigo em Português | LILACS-Express | ID: biblio-1024248

RESUMO

Introdução: O cuidador informal (CI) presta cuidados a pessoas dependentes, não sendo recompensado economicamente. São inúmeros os fatores de estresse inerentes à tarefa de cuidar, com consequente desgaste físico e emocional. Estudos indicam que as abordagens que englobam a realização de atividade física apresentam um impacto positivo na diminuição da depressão, estresse, raiva e sobrecarga apresentados pelo CI. Objetivo: Reduzir a sobrecarga do CI, com vista à capacitação da tarefa de cuidar, pela aquisição de ferramentas na gestão da sobrecarga. Métodos: De uma listagem de 90 utentes com a codificação "demência" e 50 com "atraso mental" realizou-se o diagnóstico de situação através da aplicação da escala de Zarit que avalia a sobrecarga dos CI. Destes, 13 mostraram interesse em participar na intervenção, que consistiu em sessões de educação para a saúde, sessões de yoga e dinamização de um grupo de apoio. Foi avaliado o grau de sobrecarga dos participantes e a sua satisfação. Resultados: Os CI eram maioritariamente mulheres (92%), com idade média de 62 anos. Foram realizadas 100% das sessões previstas, com 23% de desistência dos participantes. A sobrecarga moderada a severa ou severa diminuiu de 92.3% para 60% no final da intervenção. Todos os CI classificaram o projeto como "bom" ou "muito bom". Discussão: O projeto cumpriu a planificação inicial, tendo-se verificado uma diminuição do grau de sobrecarga do CI como evidenciado em outros estudos. Destaca-se como limitação a dificuldade do CI na realização de outras atividades além de cuidar do seu dependente. Conclusão: A intervenção teve um impacto positivo nos CI, tendo cumprido os objetivos inicialmente propostos. A equipa de saúde deu continuidade a este projeto através de uma parceria com uma cooperativa de solidariedade social local.


Introduction: The informal caregiver (IC) provides assistance to disabled people without any financial gratification. Many stress factors are associated with the task of taking care of others, leading to physical and emotional deterioration. Studies indicate that approaches involving physical activity have a positive impact on the reduction of depression, stress, anger and overload presented by the IC. Objective: To reduce IC overload with a view to enabling the task of caring by acquiring tools for overload management. Methods: The data collection of ICs overload was made using Zarit scale from a previous database of 90 patients classified as "dementia" and 50 as "mental retardation". 13 IC's express their interest to be part of the project. The intervention consisted in health education sessions; yoga classes; establishment of a support group. The participant's degree of overload and their satisfaction were assessed. Results: IC were mainly women (92%), with average age of 62 years. All scheduled sessions took place, with 23% participants withdrawing. Moderate to severe or severe overload level reduced from 92.3% to 60% at the end of the intervention and all participants classified the project as "good" or "very good". Discussion: The project fulfilled the initial planning. Decrease of the IC overload was noted as evidenced in other studies. The limitation is the difficulty of IC in performing other activities besides then taking care of its dependent. Conclusions: The intervention had a positive impact in the ICs and the main goals were achieved. The health team continued this project through a partnership with a local social solidarity cooperative.


Introducción: El cuidador informal (CI) presta cuidados a las personas dependientes, no siendo recompensado económicamente. Son innumerables los factores de estrés inherentes a la tarea de cuidar, con consiguiente desgaste físico y emocional. Los estudios indican que los enfoques que incluyen la actividad física tienen un impacto positivo en la reducción de la depresión, el estrés, la ira y la sobrecarga que presenta el CI. Objetivo: Reducir la sobrecarga del CI, con miras a la capacitación de la tarea de cuidar y la adquisición de herramientas en la gestión de la sobrecarga. Métodos: De una lista de 90 pacientes con la clasificación "demencia" y 50 con "retraso mental" se realizó el diagnóstico de sobrecarga del CI, aplicándose la escala de Zarit. De ellos, 13 mostraron interés en participar en la intervención, que consistió en sesiones de educación para la salud, sesiones de yoga y dinamización de un Grupo de Apoyo. Se evaluó el grado de sobrecarga de los participantes y su satisfacción. Resultados: Los CI fueron en su mayoría mujeres (92%), con una edad promedio de 62 años. El 100% de las sesiones se llevaron a cabo, y el 23% de los participantes se rindieron. La sobrecarga moderada a severa o severa disminuyó del 92,3% al 60% al final de la intervención. Todos los CIs calificaron el proyecto como "bueno" o "muy bueno". Discusión: El proyecto cumplió la planificación inicial, habiéndose comprobado una disminución del grado de sobrecarga del CI como lo demuestran otros estudios. Se destaca como limitación la dificultad del CI en la realización de otras actividades además de cuidar de su dependiente. Conclusión: La intervención tuvo un impacto positivo en los CI, habiendo cumplido los objetivos inicialmente propuestos. El equipo de salud continuó este proyecto a través de una asociación con una cooperativa de solidaridad social local.

7.
J Biomed Mater Res B Appl Biomater ; 107(5): 1393-1399, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30267641

RESUMO

This study aims to understand microorganisms' effect and relevance of aseptic conditions on wear performance of dental materials. Tribocouple zirconia/titanium was submitted to tribological tests in the presence of Streptococcus salivarius biofilm, the most abundant bacteria in the oral cavity. Reciprocating ball-on-plate tests were carried out at 37°C, pH 7, 2 N, and 1 Hz, during 2 h, using Ti6Al4V balls as pins and Y-TZP plates. Simultaneous OCP readings assessed corrosion tendency. Tested lubricants were artificial saliva, artificial saliva plus glucose, and artificial saliva plus glucose and S. salivarius. Wear volume and worn surfaces were analyzed after test. S. salivarius proliferation was enhanced in artificial saliva plus glucose. When a highly populated biofilm was present at the zirconia/titanium interface, titanium wear decreased by ~60% compared to artificial saliva and artificial saliva plus with glucose, respectively, with 0.0060 and 0.0053 mm3 loss. Wear was not observed in zirconia under any tested condition. Presence of S. salivarius also decreased corrosion activity during wear. Lowest free OCP value reached during sliding was -260 mV in artificial saliva, -246 mV in artificial saliva plus glucose, and -196 mV in artificial saliva plus glucose and bacteria. This decreased to -147 mV when a highly-populated S. salivarius biofilm was present at the interface. Overall, obtained results show that the presence of S. salivarius clearly changes the corrosion-wear performance of the tested dental materials, confirming that it must be recognized, and that asepsis level and conditions during wear test of dental materials must be clearly established and controlled to ensure reproducible results and supported conclusions. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1393-1399, 2019.

8.
BMC Vet Res ; 14(1): 95, 2018 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-29540169

RESUMO

BACKGROUND: Most of surgical site infections (SSI) are caused by commensal and pathogenic agents from the patient's microbiota, which may include antibiotic resistant strains. Pre-surgical asepsis of the skin is one of the preventive measures performed to reduce SSI incidence and also antibiotic resistance dissemination. However, in veterinary medicine there is no agreement on which biocide is the most effective. The aim of this study was to evaluate the effectiveness of two pre-surgical skin asepsis protocols in dogs. A total of 46 animals were randomly assigned for an asepsis protocol with an aqueous solution of 7.5% povidone-iodine or with an alcoholic solution of 2% chlorhexidine. For each dog, two skin swab samples were collected at pre-asepsis and post-asepsis, for bacterial quantification by conventional techniques and isolation of methicillin-resistant species. RESULTS: Most samples collected at the post-asepsis did not present bacterial growth, both for the animals subjected to the povidone-iodine (74%) or to the chlorhexidine (70%) protocols. In only 9% of the cases a significant bacterial logarithmic reduction was not observed, indicating possible resistance to these agents. Also, the logarithmic reduction of the bacterial quantification from pre- and post-asepsis time, was not statistically different for povidone-iodine (6.51 ± 1.94 log10) and chlorhexidine (6.46 ± 2.62 log10) protocol. From the 39% pre-asepsis swabs which showed bacterial growth in MRSA modified chromogenic agar medium, only one isolate was identified as Staphylococcus aureus and one as S. epidermidis. False positives were mainly other staphylococci species, as well as Enterobacteriaceae. CONCLUSIONS: Pre-surgical skin asepsis protocols with povidone-iodine or chlorhexidine showed similar efficacy in the elimination of methicillin resistant bacteria and preventing surgical site infections in dogs undergoing surgery.


Assuntos
Anti-Infecciosos Locais/uso terapêutico , Clorexidina/uso terapêutico , Doenças do Cão/prevenção & controle , Etanol/uso terapêutico , Povidona-Iodo/uso terapêutico , Infecção da Ferida Cirúrgica/veterinária , Administração Cutânea , Animais , Anti-Infecciosos Locais/administração & dosagem , Clorexidina/administração & dosagem , Protocolos Clínicos , Cães/cirurgia , Etanol/administração & dosagem , Feminino , Masculino , Povidona-Iodo/administração & dosagem , Infecção da Ferida Cirúrgica/prevenção & controle
9.
J Sci Food Agric ; 98(10): 3943-3952, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29385238

RESUMO

BACKGROUND: Borojoa sorbilis (Ducke) is an Amazonian species with edible fruits that are widely consumed by the local population, but little studied and not yet economically explored. Thus the aim of this study was to describe the chemical composition, volatile compounds, nutritional aspects and antioxidant activity of the fruit pulp and peel of B. sorbilis. RESULTS: Headspace solid-phase microextraction, using polydimethylsiloxane-divinylbenzene (PDMS-DVB) fiber, resulted in the identification of 59 substances in the pulp and peel of B. sorbilis fruits after analysis of the chromatograms obtained by gas chromatography-mass spectrometry using polar and nonpolar columns. Esters were the most abundant. Moisture, lipids, protein, dietary fiber, ash, carbohydrate, total energy value, titratable acid, soluble solids and pH were measured. Protein amount, fat content and antioxidant activity were low in both pulp and peel. Carbohydrate content was 179.2 and 134.9 g kg-1 in pulp and peel, respectively. CONCLUSION: This is the first report on the chemical characteristics, flavor and nutritional aspects of B. sorbilis fruit, which is essential to its economic exploitation. The high energy value associated with the carbohydrate content, plus the low fat content, contribute to a possible use of B. sorbilis fruits as a food supplement. © 2018 Society of Chemical Industry.


Assuntos
Frutas/química , Extratos Vegetais/química , Rubiaceae/química , Compostos Orgânicos Voláteis/química , Brasil , Fibras na Dieta/análise , Cromatografia Gasosa-Espectrometria de Massas , Valor Nutritivo
10.
ACG Case Rep J ; 4: e82, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28670596

RESUMO

Actinomycosis is a rare but easily curable infection that should be considered in the differential diagnosis of perianal fistulizing disease. We present the case of a 26-year-old woman with complex perianal fistulae, including trans-sphincteric and suprasphincteric fistulous tracts and a rectovaginal fistula, requiring multiple abscess drainages, seton placement, and antibiotic courses, with little improvement. After extensive investigation, Actinomyces spp. was identified in anal cytology. The patient underwent a 6-week course of intravenous penicillin followed by oral amoxicillin, with remarkable improvement. This case illustrates the importance of pursuing less common diagnoses in refractory complex perianal disease, such as actinomycosis.

11.
Mediators Inflamm ; 2017: 6573802, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28638180

RESUMO

Keloids are characterized by excessive collagen deposition and growth beyond the edges of the initial injury, and cytokines may be related to their formation. The objective of this study was to evaluate the collagen fibers, analyze in situ expression of cytokines in keloid lesions, and compare to the control group. Results showed that there was a predominance of women and nonwhite and direct black ancestry. Keloid showed a significant increase in total and type III collagen. Significantly, the expression of mRNA for TGF-ß in keloid was increased, the expressions of IFN-γ, IFN-γR1, and IL-10 were lower, and IFN-γR1 and TNF-α had no statistical difference. Correlations between collagen type III and TGF-ß mRNA expression were positive and significant, IFN-γ, IFN-γR1, and IL-10 were negative and significant, and TNF-α showed no statistical difference. We conclude that there was a significant increase of total collagen in keloid and predominance of collagen type III compared to the controls, showing keloid as an immature lesion. There is a significant increase in TGF-ß mRNA in keloid lesions, and a significant decrease in IFN-γ and IL-10, suggesting that these cytokines are related to keloid lesions.


Assuntos
Colágeno Tipo III/metabolismo , Colágeno Tipo I/metabolismo , Colágeno/metabolismo , Citocinas/metabolismo , Queloide/metabolismo , Adolescente , Adulto , Feminino , Humanos , Interferon gama/metabolismo , Interleucina-10/metabolismo , Masculino , Pessoa de Meia-Idade , RNA Mensageiro/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Adulto Jovem
12.
J Dairy Res ; 84(1): 80-88, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28007038

RESUMO

Bovine mastitis is the most costly disease for dairy farmers, hence, control measures to prevent it are crucial for dairy farm sustainability. Staphylococcus aureus is considered a major mastitis pathogen because of its impact on milk quality and low cure rates. Prevention of S. aureus mastitis includes segregation of infected animals, whilst treatment of such animals should be performed for a longer time to improve cure rates. This makes identification of S. aureus infected quarters and animals of significant importance. The experiments reported in this research paper aimed to develop and validate a sensitive method for magnetic detection of S. aureus and of the Staphylococcus genus in raw milk samples. Mastitic milk samples were collected aseptically from 47 cows with subclinical mastitis, from 12 Portuguese dairy farms. Forty nine quarter milk samples were selected based on bacteriological results. All samples were submitted to PCR analysis. In parallel, these milk samples were mixed with a solution combining specific antibodies and magnetic nanoparticles, to be analysed using a lab-on-a-chip magnetoresistive cytometer, with microfluidic sample handling. The antibodies used in this work were a rabbit polyclonal IgG anti-S. aureus ScpA protein and a mouse monoclonal IgM anti-S. aureus ATCC 29740. This paper describes the methodology used for magnetic detection of bacteria, including analysis of false positive/negative results. This immunological recognition was able to detect bacterial presence above 100 cfu/ml, independently of antibody and targeted bacteria used in this work. Comparison with PCR results showed sensitivities of 57·1 and 79·3%, specificity values of 75 and 50%, and PPV values of 40 and 95·8% for magnetic identification of Staphylococci species with an anti-S. aureus antibody and an anti-Staphylococcus spp. antibody, respectively. Some constraints are described as well as the method's limitations in bacterial quantification. Sensitivities and specificities require to be improved, nevertheless, the methodology described may form the basis for a means of identifying S. aureus infected cows at the point of care.


Assuntos
Fenômenos Magnéticos , Leite/microbiologia , Staphylococcus aureus/isolamento & purificação , Staphylococcus/isolamento & purificação , Animais , Anticorpos Monoclonais , Bovinos , Indústria de Laticínios , Feminino , Imunoensaio/métodos , Imunoglobulina G , Imunoglobulina M , Mastite Bovina/microbiologia , Camundongos , Nanopartículas , Reação em Cadeia da Polimerase/veterinária , Coelhos , Sensibilidade e Especificidade , Infecções Estafilocócicas/veterinária , Staphylococcus/classificação , Staphylococcus aureus/imunologia
13.
Springerplus ; 5(1): 1959, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27933239

RESUMO

PURPOSE: Surgical intestinal resection margins in colon cancer are a longstanding debate in terms the optimal distance between the tumor and the colonic section line. The aim of this study is to define the oncological outcomes in relation to surgical margins, measured in terms or recurrence rate, time-to-recurrence, disease-free survival and overall survival in a population of node negative colon cancer patients. METHODS: We conducted a retrospective observational longitudinal single institution study. All patients submitted to colon cancer surgery between January 2006 and December 2010 were analyzed. Only node negative patients were included in the study, with analysis of 215 patient charts, divided in two groups (Intestinal margin lower than 5 cm-group 1; and 5 cm or higher-group 2). RESULTS: Mean age of patients was 70.4 years (±11.7), with a male predominance (57.7%). Group 2 more frequently corresponded to Stage II (83 vs 71%; p = 0.05). Global mean total lymph nodes harvested were 12, and were higher in group II than in group I (13.8 ± 8.2 vs 10.4 ± 5.7; p = 0.001). In terms of time-to-recurrence patients of group 2 had longer time than patients of group 1 (32.3 ± 12.1 vs 21.8 ± 13.8 months; p = 0.03), as well as a lower recurrence rate in group I (13.7 vs 17.2%), despite not statistically significant. CONCLUSIONS: This study has showed that patients with 5 cm or higher bowel resection margins had longer time-to-recurrence that was statistically significant. Recurrence rates were lower in the group of patients with longer surgical margins, however not statistically significant.

14.
Biosensors (Basel) ; 6(2): 19, 2016 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-27128950

RESUMO

Bovine mastitis is the most costly disease for dairy farmers and the most frequent reason for the use of antibiotics in dairy cattle; thus, control measures to detect and prevent mastitis are crucial for dairy farm sustainability. The aim of this study was to develop and validate a sensitive method to magnetically detect Streptococcus agalactiae (a Group B streptococci) and Streptococcus uberis in raw milk samples. Mastitic milk samples were collected aseptically from 44 cows with subclinical mastitis, from 11 Portuguese dairy farms. Forty-six quarter milk samples were selected based on bacterial identification by conventional microbiology. All samples were submitted to PCR analysis. In parallel, these milk samples were mixed with a solution combining specific antibodies and magnetic nanoparticles, to be analyzed using a lab-on-a-chip magnetoresistive cytometer, with microfluidic sample handling. This paper describes a point of care methodology used for detection of bacteria, including analysis of false positive/negative results. This immunological recognition was able to detect bacterial presence in samples spiked above 100 cfu/mL, independently of antibody and targeted bacteria used in this work. Using PCR as a reference, this method correctly identified 73% of positive samples for streptococci species with an anti-S. agalactiae antibody, and 41% of positive samples for an anti-GB streptococci antibody.


Assuntos
Técnicas Biossensoriais , Microbiologia de Alimentos/métodos , Nanopartículas de Magnetita , Leite/microbiologia , Streptococcus , Animais , Bovinos , Inocuidade dos Alimentos , Humanos , Microfluídica , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Streptococcus/genética , Streptococcus/isolamento & purificação , Streptococcus agalactiae/genética , Streptococcus agalactiae/isolamento & purificação
15.
Case Rep Surg ; 2016: 7056567, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27088029

RESUMO

Introduction. Pancreatic pseudocysts are a common complication of acute pancreatitis. Pancreatic pseudocyst's natural history ranges between its spontaneous regression and the settlement of serious complications if untreated, such as splenic complications, hemorrhage, infection, biliary complications, portal hypertension, and rupture. The rupture of a pancreatic pseudocyst to the peritoneal cavity is a dangerous complication leading to severe peritonitis and septic conditions. It requires emergent surgical exploration that is often of great technical difficulty and with important morbidity and mortality. Case Study. We present two cases of spontaneous rupture of pancreatic pseudocysts, managed differently according to the local and systemic conditions. Conclusion. The best surgical choice is the internal drainage of the cyst to the GI tract; however, in some conditions, the external drainage is the only choice available.

16.
Trab. educ. saúde ; 13(1): 45-66, Jan-Apr/2015. tab
Artigo em Português | LILACS | ID: lil-733099

RESUMO

Este artigo discute a parceria do Estado com organizações sociais para a gestão do trabalho em saúde no setor público, com ênfase no trabalho do agente comunitário de saúde. Trata-se de um estudo de caso realizado no município do Rio de Janeiro numa conjuntura de expansão da cobertura da Estratégia Saúde da Família. O tema é analisado num contexto mais amplo de reforma do Estado brasileiro, partindo-se do entendimento de que o movimento reformista da administração pública, conhecido como gerencialismo, guarda estreita relação nas suas ações e valores com a reestruturação produtiva iniciada nos anos 1970. Os resultados mostram que o modelo de gestão do trabalho implantado opera sob a lógica da produtividade, influenciando o modelo de atenção à saúde prestado à população e a organização do trabalho do agente comunitário de saúde. A análise também destaca a temporalidade e a externalidade dos vínculos de trabalho como fatores que podem comprometer os avanços da democratização, equidade e cidadania no campo da saúde e do trabalho conquistados na Constituição brasileira de 1988.


This article discusses the partnership between the State and social organizations for the management of health work in the public sector, with emphasis on the work of the community health agent. This is a case study carried out in the municipality of Rio de Janeiro, Brazil, in an environment of expansions in the coverage of the Family Health Strategy. The topic is analyzed in a context of a broader reform of the Brazilian State, starting from the understanding that the public administration reform movement, known as managerialism, is closely related in their actions and values with the productive restructuring that got underway in the 1970s. The results show that the work management model that was deployed operates under the logic of productivity, influencing the model of the health care provided to the population and the organization of the work done by the community health agent. The analysis also highlights the temporality and externality of the work ties as factors that can compromise the progress of democratization, equity, and citizenship in the field of health and work ensured by the Brazilian Constitution of 1988.


Este artículo discute la asociación del Estado con organizaciones sociales para la gestión del trabajo en salud en el sector público, con énfasis en el trabajo del agente comunitario de salud. Se trata de un estudio de caso realizado en el municipio de Río de Janeiro, Brasil, en una coyuntura de expansión de la cobertura de la Estrategia Salud Familiar. El tema se analiza en un contexto más amplio de reforma del Estado brasileño, partiendo de la base de que el movimiento reformista de la administración pública, conocido como gerencialismo, guarda estrecha relación en sus acciones y valores con la restructuración productiva iniciada en los años 70. Los resultados muestran que el modelo de gestión del trabajo implantado opera bajo la lógica de la productividad, influyendo sobre el modelo de atención de la salud prestado a la población y la organización del trabajo del agente comunitario de salud. El análisis también destaca la temporalidad y la externalidad de los vínculos de trabajo como factores que pueden comprometer los avances de la democratización, equidad y ciudadanía en el campo de la salud y del trabajo conquistados en la Constitución brasileña de 1988.


Assuntos
Humanos , Organização e Administração , Trabalho , Saúde , Agentes Comunitários de Saúde , Modernização do Setor Público
17.
Environ Monit Assess ; 187(3): 96, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25663399

RESUMO

Honey is used as an alternative medicine and is a constituent of a healthy diet worldwide. Its composition is associated with botanical origin and, to some extent, geographical origin because soil and climate characteristics determine the melliferous flora. Also, the elements content in honey samples could give an indication of environmental pollution or geographical origin. The main purpose of this study was to evaluate seasonal patterns of essential elements of Brazilian honey. Honey was collected during spring, summer, autumn, and winter for 2 years to quantify K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Se, Br, and Sr using total reflection X-ray fluorescence spectroscopy (TXRF). Our results indicate no seasonal differences in concentration of Cr, Ni, Se, and Ti, although there were significant seasonal patterns in the composition of essential elements in honey, with higher concentrations of minor and trace elements, especially K and Ca of samples collected in spring and summer.


Assuntos
Mel/análise , Oligoelementos/análise , Brasil , Monitoramento Ambiental , Fluorescência , Geografia , Estações do Ano , Espectrometria por Raios X , Raios X
18.
Int J Colorectal Dis ; 30(2): 173-9, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25430595

RESUMO

PURPOSE: This study aims to compare the predictive value of POSSUM, P-POSSUM, CR-POSSUM and CR-BHOM in colorectal surgical mortality and morbidity in patients over 80 years old. METHODS: This is a retrospective observational longitudinal study. A total of 991 patients who underwent major colorectal surgery between 2008 and 2012 in a secondary hospital in Portugal were screened, and 204 who were over 80 years old were included. Subgroup analysis was performed for malignant/benign disease and emergent/elective surgery. The main outcome measure was 30-day postoperative mortality and morbidity with Clavien-Dindo classification ≥ 2. RESULTS: Of the 204 patients included in this study, 155 had malignant disease, and 65 underwent emergent procedures. Overall average age was 84.3 ± 3.9 years (range 80-100). Overall surgical mortality and morbidity were 18.6% (n = 38) and 52.4% (n = 87), respectively. Expected mortality followed the order P-POSSUM

Assuntos
Cirurgia Colorretal/mortalidade , Morbidade , Idoso de 80 Anos ou mais , Procedimentos Cirúrgicos Eletivos , Tratamento de Emergência , Feminino , Humanos , Modelos Logísticos , Estudos Longitudinais , Masculino , Estudos Retrospectivos , Sensibilidade e Especificidade
19.
J Food Sci ; 79(4): T738-42, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24712495

RESUMO

Trace and minor elements in Brazilian honey were analyzed by total reflection X-ray fluorescence spectroscopy. Up to 12 elements (K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Se, Br, and Sr) were detected in 160 samples of honey from 4 regions of Rio de Janeiro State (Barra Mansa, Teresópolis, northern and southern Nova Friburgo). The results showed the samples from Teresópolis had higher rates of essential and nonessential elements than samples from the other regions, except for Ni. K and Ca were the most abundant elements in all samples, in the range of 116.5 to 987.0 µg g(-1) . Ni, Cu, Zn, Se, and Sr were identified in small concentrations (0.01 to 12.08 µg g(-1) ) in all samples, indicating a low level of contamination in all the regions.


Assuntos
Dieta , Exposição Ambiental/análise , Contaminação de Alimentos/análise , Mel/análise , Metais Pesados/análise , Oligoelementos/análise , Brasil , Fluorescência , Humanos , Espectrometria por Raios X , Raios X
20.
J Dairy Res ; 81(2): 208-14, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24594229

RESUMO

The objectives of this study were to compare the impact of different coagulase-negative species (CNS) on udder health measured in terms of individual quarter milk somatic cell count (SCC) and duration of intramammary infection, and to get some insight into most likely routes of infection for different CNS species. This longitudinal observational study was performed on four farms that were sampled at 4-week intervals for a total of 12 visits each. Quarters infected with CNS were followed through time with milk samples being submitted for bacteriological culture and SCC determination. PCR amplification of the internal transcribed spacer region and sequencing of the sodA and rpoB genes were used for species allocation. Pulsed-field gel electrophoresis (PFGE) was performed to assess strain identity. The percentage of quarters affected per farm varied between 6 and 35%, with the most frequently isolated CNS species being Staphylococcus epidermidis, followed by Staph. simulans, Staph. chromogenes and Staph. haemolyticus. It was possible to follow 111 intramammary infections due to CNS through time. Duration of infection had a mean of 188 d and was not significantly different between CNS species. Geometric mean quarter SCC overall was 132 000 cells/ml and was also not significantly different between CNS species. Despite the possibility of a different epidemiology of infection, the impact in terms of udder health seems to be similar for different CNS species.


Assuntos
Coagulase/análise , Indústria de Laticínios , Mastite Bovina/microbiologia , Infecções Estafilocócicas/veterinária , Staphylococcus/classificação , Animais , Bovinos , Contagem de Células , DNA Bacteriano/análise , Feminino , Genótipo , Humanos , Estudos Longitudinais , Glândulas Mamárias Animais/microbiologia , Leite/citologia , Reação em Cadeia da Polimerase , Infecções Estafilocócicas/microbiologia , Staphylococcus/enzimologia , Staphylococcus/genética , Fatores de Tempo
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