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1.
Photodiagnosis Photodyn Ther ; 34: 102255, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33727132

RESUMO

INTRODUCTION: Oral lichen planus (OLP) is a common oral inflammatory condition. Against symptomatic atrophic-erosive OLP, topical steroids, or photobiomodulation (PBM) are deployed. Optical coherence tomography (OCT) provides a real-time, non-invasive, tissue investigation. Aim of this study was to evaluate modifications of OCT pattern in patients with painful atrophic-erosive OLP, before and after treatment with PBM, comparing those results with patients treated with topical steroid. METHODS: Two groups of 20 OLP patients were evaluated. Group A underwent two daily application of 0.05 % clobetasol propionate for 8 weeks; group B was treated with eight weekly PBM sessions using a 980/645 nm diode laser. OCT scans were performed before and after treatment, and six months after end of the proposed protocol. Changes of width of stratified epithelium (EP) and lamina propria (LP) were quantified. RESULTS: After 8-weeks, both groups experienced a significant increase of EP width (p < 0.05), and a significant decrease of LP width (p < 0.05), with Δ-EP in Group A significantly higher than Group B (p = 0.0015); conversely, Δ-LP was not significantly different (p > 0.05). After six months, significant increase of EP width remained only in group B (p = 0.01), with no significant decrease of LP mean width in both groups (p > 0.05). CONCLUSIONS: Increase of EP and decrease of LP might be explained as consequence of clobetasol and PBM ability to promote epithelial healing, and to reduce interface inflammation. When investigated with OCT, clobetasol appears to provide more significant short-term structural changes, whereas PBM might guarantee long-term alterations.


Assuntos
Líquen Plano Bucal , Fotoquimioterapia , Administração Tópica , Anti-Inflamatórios/uso terapêutico , Glucocorticoides/uso terapêutico , Humanos , Líquen Plano Bucal/tratamento farmacológico , Líquen Plano Bucal/terapia , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/uso terapêutico , Tomografia de Coerência Óptica
2.
J Oral Maxillofac Surg ; 79(2): 305-312, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33091403

RESUMO

PURPOSE: Infections foster morbidity and mortality after liver transplantation (LT). Pre-LT eradication of oral infectious foci is not always possible for patients needing an urgent LT because postextraction sockets must be healed before the patient is operated, and this requires at least 3 weeks. To accelerate healing, we tested the effect of plasma-rich growth factor (PRGF), a highly concentrated form of autogenous platelets on healing. MATERIALS AND METHODS: Prospective case-control split-mouth study for more than 100 candidates for LT needing routine extractions of 2 homologous teeth: a socket was to be treated with PRGF, whereas its match (control [CTRL]) was to undergo natural healing. The outcome of interest was the socket size derived from the measurements on the transversal diameters and deepest level of penetration on the PRGF and CTRL sides after extraction and on day 7, 14, and 21 postextraction. The primary predictor was treatment status (PRGF vs CTRL); secondary predictors, the tooth extracted and patient's features. The statistical analysis used nonparametric tests and best subset regression. RESULTS: All measurements evidenced a significantly (P < .0001) more advanced closure on the PRGF side than the CTRL side. One week after extraction, PRGF sockets were reduced to 12% (molars) and 6% (nonmolars) of the original wound versus 32 and 20% for CTRL, respectively. The percentage of PRGF sockets with size less than or equal to 5% was 7% for molars and 44% for nonmolars versus 0 and 12% for CTRL (P < .0001), respectively. The percentages with size less than or equal to 10% were 37% for molars and 81% for nonmolars on the PRGF side versus 2 and 26% on the CTRL side, respectively. These percentages showed a significant decrease for smoking patients. CONCLUSIONS: The outcome of our trial showed that PRGF significantly accelerates closure of postextraction sockets. Its use, at least in patients who occupy top positions in the LT waiting list, is recommended.


Assuntos
Transplante de Fígado , Alvéolo Dental , Humanos , Peptídeos e Proteínas de Sinalização Intercelular , Plasma , Estudos Prospectivos , Extração Dentária , Alvéolo Dental/cirurgia
3.
BMC Oral Health ; 20(1): 271, 2020 10 07.
Artigo em Inglês | MEDLINE | ID: mdl-33028288

RESUMO

BACKGROUND: Fully adjustable articulators and pantographs record and reproduce individual mandibular movements. Although these instruments are accurate, they are operator-dependant and time-consuming. Pantographic recording is affected by inter and intra operator variability in the individuation of clinical reference points and afterwards in reading pantographic recording themselves. Finally only border movements can be reproduced. METHODS: Bionic Jaw Motion system is based on two components: a jaw movement analyzer and a robotic device that accurately reproduces recorded movements. The jaw movement analyzer uses an optoelectronic motion system technology made of a high frequency filming camera that acquires 140frames per second and a custom designed software that recognizes and determines the relative distance at each point in time of markers with known geometries connected to each jaw. Circumferential modified retainers connect markers and do not cover any occlusal surfaces neither obstruct occlusion. The recording process takes 5 to 10 s. Mandibular movement performance requires six degrees of freedom of movement, 3 rotations and 3 translations. Other robots are based on the so-called delta mechanics that use several parallel effectors to perform desired movements in order to decompose a complex trajectory into multiple more simple linear movements. However, each parallel effector introduces mechanical inter-component tolerances and mathematical transformations that are required to transform a recorded movement into the combination of movements to be performed by each effector. Bionic Jaw Motion Robot works differently, owing to three motors that perform translational movements and three other motors that perform rotations as a gyroscope. This configuration requires less mechanical components thus reducing mechanical tolerances and production costs. Both the jaw movement analyzer and the robot quantify the movement of the mandible as a rigid body with six degrees of freedom. This represents an additional advantage as no mathematical transformation is needed for the robot to reproduce recorded movements. RESULTS: Based on the described procedure, Bionic Jaw Motion provide accurate recording and reproduction of maxillomandibular relation in static and dynamic conditions. CONCLUSION: This robotic system represents an important advancement compared to available analogical and digital alternatives both in clinical and research contexts for cost reduction, precision and time saving opportunities.


Assuntos
Robótica , Articuladores Dentários , Registro da Relação Maxilomandibular , Mandíbula , Movimento , Reprodução
4.
J Photochem Photobiol B ; 211: 112009, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32862089

RESUMO

Oral lichen planus (OLP) is a common premalignant chronic inflammatory disorder. Optical Coherence Tomography (OCT) provides a real-time, non-invasive, and in-situ optical signature using light of varying wavelengths to examine tissue. Aim of the present study was to assess the possible role of OCT as diagnostic tool for atrophic-erosive OLP by examining OCT scans of healthy buccal mucosa, and comparing their ultrastructural features with those of a buccal mucosa affected by atrophic-erosive OLP, using their histopathological counterparts as the gold standard. Through grayscale (enface scan) and an application in which the vascularization of the tissue is visible (dynamic scan), it was possible to distinguish the healthy from the lichenoid pattern from 20 controls (12 M; 8 F; mean age: 41.32 years) and 20 patients with histologically confirmed atrophic-erosive OLP (7 M; 13 F; mean age: 64.27 years). In detail, mean width of stratified squamous epithelium (EP) and lamina propria (LP) were evaluated. Among controls, EP and LP showed a mean width of 300 (±50) and of 600 (±50) µm respectively; among cases, disruption of membrane basement prevented from any measurement. Furthermore, a differential pattern of EP and LP emerged between the two groups: a light-grayish, hypo-reflective, homogeneous area of EP recurring in controls turned into a hyper-reflective, non-homogeneous area among cases. Dynamic scan showed a differential profile of LP vascularization, varying from a hypo-reflective red area with small blood vessels in the control group, to a hypo/hyper-reflective area, completely overrun by a denser, wider blood flow amid OLP cases. Although histopathological examination remains the gold standard for OLP diagnosis, OCT could be a potentially helpful tool for the clinician and the pathologist, since it allows analysis of the vascularization of the sample without adversely affecting histological processing.


Assuntos
Líquen Plano Bucal/tratamento farmacológico , Mucosa Bucal/diagnóstico por imagem , Tomografia de Coerência Óptica/métodos , Adulto , Biópsia , Feminino , Humanos , Cinética , Líquen Plano Bucal/patologia , Luz , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/fisiologia , Mucosa Bucal/ultraestrutura , Lesões Pré-Cancerosas/metabolismo
6.
Int J Mol Sci ; 21(6)2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32168919

RESUMO

BACKGROUND: Attaining an effective mucosal attachment to the transmucosal part of the implant could protect the peri-implant bone. AIM: To evaluate if chair side surface treatments (plasma of Argon and ultraviolet light) may affect fibroblast adhesion on different titanium surfaces designed for soft tissue healing. METHODS: Grade 5 titanium discs with four different surface topographies were subdivided into 3 groups: argon-plasma; ultraviolet light, and no treatment. Cell morphology and adhesion tests were performed at 20 min, 24 h, and 72 h. RESULTS: Qualitative observation of the surfaces performed at the SEM was in accordance with the anticipated features. Roughness values ranged from smooth (MAC Sa = 0.2) to very rough (XA Sa = 21). At 20 min, all the untreated surfaces presented hemispherical cells with reduced filopodia, while the cells on treated samples were more spread with broad lamellipodia. However, these differences in spreading behavior disappeared at 24 h and 72 h. Argon-plasma, but not UV, significantly increased the number of fibroblasts independently of the surface type but only at 20 min. Statistically, there was no surface in combination with a treatment that favored a greater cellular adhesion. CONCLUSIONS: Data showed potential biological benefits of treating implant abutment surfaces with the plasma of argon in relation to early-stage cell adhesion.


Assuntos
Argônio/farmacologia , Fibroblastos/citologia , Titânio/química , Adesão Celular , Proliferação de Células/fisiologia , Células Cultivadas , Fibroblastos/efeitos dos fármacos , Humanos , Técnicas In Vitro , Propriedades de Superfície
7.
Clin Oral Investig ; 24(8): 2611-2623, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31748982

RESUMO

BACKGROUND: The osteoconductive properties of bone grafting materials represent one area of research for the management of bony defects found in the fields of periodontology and oral surgery. From a physico-chemical aspect, the wettability of the graft has been demonstrated to be one of the most important factors for new bone formation. It is also well-known that argon plasma treatment (PAT) and ultraviolet irradiation (UV) may increase the surface wettability and, consequently, improve the regenerative potential of the bone grafts. Therefore, the aim of the present in vitro study was to evaluate the effect of PAT and UV treatment on the osteoconductive potential of various bone grafts. MATERIALS AND METHODS: The following four frequently used bone grafts were selected for this study: synthetic hydroxyapatite (Mg-HA), biphasic calcium phosphate (BCP), cancellous and cortical xenogenic bone matrices (CaBM, CoBM). Sixty-six serially numbered disks 10 mm in diameter were used for each graft material and randomly assigned to the following three groups: test 1 (PAT), test 2 (UV), and control (no treatment). Six samples underwent topographic analysis using SEM pre- and post-treatments to evaluate changes in surface topography/characteristics. Additionally, cell adhesion and cell proliferation were evaluated at 2 and 72 h respectively following incubation in a three-dimensional culture system utilizing a bioreactor. Furthermore, the effects of PAT and UV on immune cells were assessed by measuring the viability of human macrophages at 24 h. RESULTS: The topographic analysis showed different initial morphologies of the commercial biomaterials (e.g., Mg-HA and BCP showed flat morphology; BM samples were extremely porous with high roughness). The surface analysis following experimental treatments did not demonstrate topographical difference when compared with controls. Investigation of cells demonstrated that PAT treatment significantly increased cell adhesion of all 4 evaluated bone substitutes, whereas UV failed to show any statistically significant differences. The viability test revealed no differences in terms of macrophage adhesion on any of the tested surfaces. CONCLUSION: Within their limitations, the present results suggest that treatment of various bone grafting materials with PAT appears to enhance the osteoconductivity of bone substitutes in the early stage by improving osteoblast adhesion without concomitantly affecting macrophage viability. CLINICAL RELEVANCE: Treatment of bone grafts with PAT appears to result in faster osseointegration of the bone grafting materials and may thus favorably influence bone regeneration.


Assuntos
Regeneração Óssea , Substitutos Ósseos , Argônio , Materiais Biocompatíveis , Transplante Ósseo , Durapatita , Humanos , Gases em Plasma
8.
Minerva Stomatol ; 68(5): 217-225, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31822045

RESUMO

BACKGROUND: Replacement of missing teeth in the anterior aesthetic zone has challenged clinicians with fixed, removable or implant prosthodontics. Problems have been encountered could void aesthetic and functional results. The aim of this study was to present a clinical case report in which clinician challenged a very strong aesthetic case with an innovative surgical and prosthetic approach. METHODS: A patient who presented to the authors with a maxillary central incisor that had a hopeless prognosis secondary to acute localized severe periodontitis. The patient was treated by extracting the tooth and replacing it immediately with a platform switched implant using piezoelectric surgery. The implant was then restored with an immediate, non-occlusal loading provisional restoration. RESULTS: The implant became osseointegrated and was ultimately restored with a definitive abutment and crown restoration. CONCLUSIONS: Innovative approaches could represent a valid alternative to restore patients functionally and aesthetically.


Assuntos
Implantes Dentários para Um Único Dente , Carga Imediata em Implante Dentário , Coroas , Implantação Dentária Endo-Óssea , Prótese Dentária Fixada por Implante , Estética Dentária , Humanos , Incisivo , Maxila , Osseointegração , Resultado do Tratamento
9.
Front Physiol ; 10: 1291, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31681005

RESUMO

The synergistic crosstalk between osteodifferentiating stem cells and endothelial cells (ECs) gained the deserved consideration, shedding light on the role of angiogenesis for bone formation and healing. A deep understanding of the molecular basis underlying the mutual influence of mesenchymal stem cells (MSCs) and ECs in the osteogenic process may help improve greatly bone regeneration. Here, the authors demonstrated that osteodifferentiating MSCs co-cultured with ECs promote angiogenesis and ECs recruitment. Moreover, through the use of 3D co-culture systems, we showed that ECs are in turn able to further stimulate the osteodifferentiation of MSCs, thus enhancing bone production. These findings highlighted the existence of a virtuous loop between MSCs and ECs that is central to the osteogenic process. Unraveling the molecular mechanisms governing the functional interaction MSCs and ECs holds great potential in the field of regenerative medicine.

10.
Minerva Stomatol ; 68(4): 177-182, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31357851

RESUMO

BACKGROUND: Peri-implantitis or implant infection is a biologic complication involving soft and hard tissues around implants. The prevalence of the disease was recently estimated between 12% and 14% according to studies dealing with the private practice, which is also consistent with university statistics. Different methods were presented to minimize or even to remove biofilm from contaminated surfaces completely. Chemical and air-abrasive treatments have been shown to be able to disrupt biofilm. Chemical cleaning solution in combination with mechanical debridement is ineffective to eliminate bacterial biofilm. Lasers and photodynamic therapy presented inconsistent results. Interestingly, implantoplasty remains a preferred way to remove infected contaminants. When re-osseointegration of these treated contaminated implant surfaces was assessed, the quality of the implant surface after decontamination dictates the outcome. No matter the type of intervention implemented to counteract peri-implantitis, implant failure sometimes remains an unavoidable outcome. In this in-vitro report, the authors propose an automated EDS analysis of the whole dental implant surface to determine the percentage directly involved by the bacterial biofilm on failed fixtures. METHODS: Samples morphology was studied using a Scanning Electron Microscope (SEM, Zeiss Evo 50 XVP with LaB6 source). The instrument is endowed with detectors for secondary and backscattered electrons collection, as well as energy dispersion spectroscopy (EDS) analyzer for elemental analysis. All the materials were observed using 10kV of voltage. Samples, soon after being collected and fixed in 4% paraformaldehyde, were covered with a golden layer of about 10 nm in order to avoid charge accumulation during SEM-EDS analysis. Automated EDS mapping was obtained on the entire surface. RESULTS: On the samples analyzed (N.=10), the mean surface covered by bacterial biofilm was 79.3±7.6% (Mean±95% CI) based on the percentage of titanium, oxygen, and phosphorous. As a control, direct observation of the samples was also performed owing to SEM images finding an optimal correlation between the automatic EDS mapping and human-driven quantification of the bacterial biofilm. CONCLUSIONS: Based on these preliminary data, EDS automatic mapping may be considered an exciting method to analyze failed implants. Furthermore, the possible future applications in this field, once the bacteria have been identified, could involve a more specific treatment with the aim of remove infected contaminants on the implant surfaces.


Assuntos
Implantes Dentários , Peri-Implantite , Biofilmes , Humanos , Análise Espectral , Propriedades de Superfície , Inquéritos e Questionários , Titânio
11.
J Oral Maxillofac Surg ; 77(8): 1557-1565, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31026420

RESUMO

PURPOSE: Eradication of oral infectious foci is essential for liver transplant candidates. The main issue is whether antibiotic prophylaxis is necessary for all dental extractions despite the possible fostering of resistant bacteria. To overcome the scarcity of evidence-based data, our study analyzed the background and outcomes of a large number of routine extractions performed in our institution without antibiotic prophylaxis in patients with different liver pathologies. MATERIALS AND METHODS: In this retrospective cohort study, the outcome of interest was the occurrence of local infections and minor complications during the 7-day follow-up period after extraction; the predictors were the demographic and clinical variables of the patients (age, gender, liver pathology, Model for End-Stage Liver Disease score, international normalized ratio) and the extraction variables (single vs multiple extractions, isolated vs contiguous teeth, single-rooted vs multirooted teeth). The statistical analysis used univariate nonparametric tests and binary multivariate logistic regressions. RESULTS: The 346 liver transplant candidates (mean age, 53 ± 8 years; 24% women) underwent 662 routine extraction sessions involving 1,329 teeth. The 7-day dental follow-up detected no signs of postoperative wound infection (rate = 0% [95% confidence interval (CI), 0 to 0.9%]). Accordingly, the 2-week post-extraction clinical monitoring excluded symptoms of systemic infection attributable to the dental procedure. Minor complications (mild bleeding, slow healing, inflamed socket) occurred in 50 patients (rate = 14% [95% CI, 11 to 18%]) in the 3 days after extraction. Significant risk factors for minor complications were refractory ascites (P < .0001; OR = 8 [95% CI, 3 to 20]), extraction of multirooted contiguous teeth (P < 0.0001; OR = 5 [95% CI, 2.5 to 9]), and a Model for End-Stage Liver Disease score greater than 18 (P = 0.01; OR = 2.4 [95% CI, 1.2 to 5]). CONCLUSIONS: Our study showed that routine extractions without antibiotic prophylaxis can be performed safely in liver transplant candidates, even in the presence of 1 or more non-controllable risk factors. Using atraumatic techniques, we achieved satisfactory healing of the gingiva and socket in all patients in a week, without any signs of local infection. The few minor complications were readily managed and resolved within 3 days after extraction.


Assuntos
Antibacterianos , Transplante de Fígado , Extração Dentária , Antibioticoprofilaxia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Extração Dentária/efeitos adversos
12.
J Oral Maxillofac Surg ; 77(3): 463-470, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30347201

RESUMO

PURPOSE: The purpose of this study was to measure the frequency of bleeding during and after tooth extraction in patients exposed to direct oral anticoagulants (DOACs) and identify risk factors for prolonged or excessive bleeding. MATERIALS AND METHODS: This retrospective cohort study involved 100 patients who underwent tooth extractions according to the European Heart Rhythm Association protocol: continuation of DOAC therapy for extractions of up to 3 teeth in the same session performed at the (presumed) time of DOAC trough concentration. We respected an interval of at least 4 hours between extraction and last DOAC intake. The outcome of interest was incidence of mild, moderate, and severe bleeding during the intervention and in the 7-day follow-up period. Data analysis considered the presence of comorbidities as the primary predictor for bleeding; additional predictors were age, gender, type of comorbidity, indication for DOAC therapy, DOAC agent, and extraction of contiguous teeth. RESULTS: Of the patients, 64 had comorbidities (diabetes in 50%). The distributions of demographic, clinical, and dental variables were similar for patients with and without comorbidities. We observed 4 bleeding episodes (1 moderate episode 1 hour after the extraction and 3 mild episodes the day after the extraction) in the comorbidity group and none in the non-comorbidity group (4 of 64 vs 0 of 36, P = .29; overall bleeding rate, 4 of 100). The factor significantly triggering bleeding in patients with comorbidity was extractions of couples and triplets of multirooted teeth (P = .004). CONCLUSIONS: Tooth extractions in patients with comorbidities taking DOACs may be safely managed as long as they are performed at least 4 hours after the last DOAC intake and do not involve 2 or 3 contiguous premolars and molars.


Assuntos
Anticoagulantes/uso terapêutico , Hemorragia/tratamento farmacológico , Extração Dentária , Administração Oral , Comorbidade , Humanos , Estudos Retrospectivos
13.
Int J Periodontics Restorative Dent ; 39(1): 115­121, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-29677223

RESUMO

This retrospective study evaluated implant survival rate (SR) and marginal bone loss around dental implants placed with ultrasonic implant site preparation. A total of 156 implants were placed in 28 totally and partially edentulous patients. Bone loss was measured on intraoral paralleling digital radiographs taken at the impression phase and after 4 years of loading. As 3 implants (1.92%) failed at the second surgery stage, the SR was 98.08% after 4 years. The mean marginal bone loss was 0.52 ± 0.33 mm (0.51 ± 0.35 mm mesially and 0.53 ± 0.35 mm distally), with comparable values for implants inserted into the maxilla (0.52 ± 0.32 mm) and the mandible (0.52 ± 0.35 mm).


Assuntos
Perda do Osso Alveolar/diagnóstico por imagem , Perda do Osso Alveolar/patologia , Implantação Dentária Endo-Óssea , Implantes Dentários , Terapia por Ultrassom , Idoso , Perda do Osso Alveolar/cirurgia , Dente Suporte , Implantes Dentários para Um Único Dente , Planejamento de Prótese Dentária , Falha de Restauração Dentária , Prótese Parcial , Feminino , Seguimentos , Humanos , Carga Imediata em Implante Dentário , Arcada Edêntula/cirurgia , Arcada Parcialmente Edêntula , Masculino , Mandíbula/cirurgia , Maxila/cirurgia , Pessoa de Meia-Idade , Radiografia , Estudos Retrospectivos , Taxa de Sobrevida , Titânio , Resultado do Tratamento , Ultrassom
14.
Biomed Res Int ; 2018: 6817154, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30186865

RESUMO

Aim: To compare implant survival rate and marginal bone loss (MBL) of immediately loaded single implants inserted by using ultrasonic implant site preparation (UISP) (test) and conventional rotary instrumentation (control). Methods: Two single implants were inserted for each patient: after randomization, test site was prepared by using an ultrasonic device (Piezosurgery Touch, Mectron, Italy) and control site was prepared by using the drills of the selected implant system (Premium AZT, Sweden & Martina, Italy), until reaching a final diameter of 3 mm in both groups. Identical implants (3.8x11.5 mm) were inserted in all sites at crestal level. Impressions were taken and screwed resin single crowns with platform-switched provisional abutments were delivered with 48 hours. Periapical radiographs were taken at provisional crown insertion (T0), 6 months (T1) and one year (T2) after prosthetic loading to measure MBL. All data were tested for normality and subsequently analyzed by paired samples t-test and forward multiple linear regression. Results: Forty-eight patients were treated in six centers with the insertion of ninety-six implants (48 test; 48 control). Four implants in four patients failed within the first six months of healing (two in test group; two in control group; no difference between groups). Forty patients (age 60.1±10.7 years; 22 female, 18 male) were included in the final analysis. Mean MBL after six months of loading was 1.39±1.03 mm in the test group and 1.42±1.16 mm in the control group (p>0.05) and after one year was 1.92±1.14 mm and 2.14±1.55 mm in test and control, respectively (p>0.05). Conclusions: No differences in survival rate and MBL were demonstrated between UISP and conventional site preparation with rotary instruments in immediately loaded dental implants: UISP, with its characteristics of enhanced surgical control and safety in proximity of delicate structures, may be used as a reliable alternative to the traditional drilling systems.


Assuntos
Implantação Dentária Endo-Óssea , Carga Imediata em Implante Dentário , Ultrassom , Adulto , Perda do Osso Alveolar , Coroas , Implantes Dentários para Um Único Dente , Falha de Restauração Dentária , Feminino , Seguimentos , Humanos , Itália , Masculino , Suécia , Resultado do Tratamento
15.
Int J Oral Maxillofac Implants ; 33(3): 571-579, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29763495

RESUMO

PURPOSE: This research aimed to assess whether pink-shaded anodized surfaces could enhance the adhesion of soft tissue cells compared with untreated machined titanium surfaces. MATERIALS AND METHODS: Two types of Ti-Al-V titanium samples were prepared: machined titanium (Ti) and anodized titanium (AnoTi). The microstructure was studied by means of a scanning electron microscope. X-ray photoelectron spectroscopy (XPS) was carried out as well. The wetting properties were investigated by the sessile drop technique with water and diiodomethane. To investigate the biologic response in vitro, the epithelial cell line HaCaT and the fibroblastic cell line NHDF were used. Cell adhesion, morphology, and proliferation were evaluated. RESULTS: The microstructure of the tested surfaces was irregularly smooth for both types of samples with no relevant morphologic differences. The XPS and HR-XPS performed on the AnoTi samples confirmed the presence of Ti, O, and C, along with Ti oxides. Following the optical contact angle measurements, the anodization process induced a slight transition toward the hydrophobic regime. Consequently, the surface free energy values differed significantly between the anodized and the machined samples. Anodized Ti significantly increased the adhesion and proliferation of both epithelial cells and fibroblasts when compared with the pristine Ti controls. CONCLUSION: Compared with the clinical standard, anodized surfaces could enhance the adhesion of the two major cell types within the peri-implant soft tissues, which makes pink anodization a promising option for implant dentistry.


Assuntos
Adesão Celular/fisiologia , Implantes Dentários , Células Epiteliais/fisiologia , Fibroblastos/fisiologia , Titânio , Linhagem Celular , Dente Suporte , Prótese Dentária Fixada por Implante , Células Epiteliais/ultraestrutura , Fibroblastos/ultraestrutura , Humanos , Microscopia Eletrônica de Varredura , Propriedades de Superfície , Titânio/química
16.
BMC Cancer ; 18(1): 439, 2018 04 18.
Artigo em Inglês | MEDLINE | ID: mdl-29669525

RESUMO

BACKGROUND: Several studies in the past have investigated the expression of micro RNAs (miRNAs) in saliva as potential biomarkers. Since miRNAs associated with extracellular vesicles (EVs) are known to be protected from enzymatic degradation, we evaluated whether salivary EVs from patients with oral squamous cell carcinoma (OSCC) were enriched with specific subsets of miRNAs. METHODS: OSCC patients and controls were matched with regards to age, gender and risk factors. Total RNA was extracted from salivary EVs and the differential expression of miRNAs was evaluated by qRT-PCR array and qRT-PCR. The discrimination power of up-regulated miRNAs as biomarkers in OSCC patients versus controls was evaluated by the Receiver Operating Characteristic (ROC) curves. RESULTS: A preliminary qRT-PCR array was performed on samples from 5 OSCC patients and 5 healthy controls whereby a subset of miRNAs were identified that were differentially expressed. On the basis of these results, a cohort of additional 16 patients and 6 controls were analyzed to further confirm the miRNAs that were up-regulated or selectively expressed in the previous pilot study. The following miRNAs: miR-302b-3p and miR-517b-3p were expressed only in EVs from OSCC patients and miR-512-3p and miR-412-3p were up-regulated in salivary EVs from OSCC patients compared to controls with the ROC curve showing a good discrimination power for OSCC diagnosis. The Kyoto Encyclopedia of Gene and Genomes (KEGG) pathway analysis suggested the possible involvement of the miRNAs identified in pathways activated in OSCC. CONCLUSIONS: In this work, we suggest that salivary EVs isolated by a simple charge-based precipitation technique can be exploited as a non-invasive source of miRNAs for OSCC diagnosis. Moreover, we have identified a subset of miRNAs selectively enriched in EVs of OSCC patients that could be potential biomarkers.


Assuntos
Carcinoma de Células Escamosas/genética , Vesículas Extracelulares/metabolismo , MicroRNAs/genética , Neoplasias Bucais/genética , Saliva/metabolismo , Adulto , Idoso , Biomarcadores , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/virologia , Feminino , Papillomavirus Humano 16 , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/metabolismo , Neoplasias Bucais/patologia , Neoplasias Bucais/virologia , Estadiamento de Neoplasias , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/virologia
17.
BMC Oral Health ; 18(1): 55, 2018 04 02.
Artigo em Inglês | MEDLINE | ID: mdl-29609564

RESUMO

BACKGROUND: Apical periodontitis includes periapical granulomas and radicular cysts, which are histologically distinguished by the absence and the presence of an epithelial lining, respectively. The main cause of apical periodontitis is the bacterial colonization of the root canal space. This research aimed at assessing whether and how periapical granulomas and radicular cysts differ in terms of microbiota using high throughput amplicon target sequencing (HTS) techniques. METHODS: This study included 5 cases of Periapical Granulomas (PGs) and 5 cases of Radicular Cysts (RCs) selected on the base of histology out of 37 patients from January 2015 to February 2016. Complete medical history, panoramic radiograms (OPTs) and histologic records of each patient were assessed. Only lesions greater than 1 cm in diameter and developed in proximity to teeth with bad prognosis were included. The microbiota present in periapical granulomas and radicular cysts thus retrieved was finely characterized by pyrosequencing of the 16S rRNA genes. RESULTS: The core of OTUs shared between periapical granulomas and radicular cysts was dominated by the presence of facultative anaerobes taxa such as: Lactococcus lactis, Propionibacterium acnes, Staphylococcus warneri, Acinetobacter johnsonii and Gemellales. L. lactis, the main OTUs of the entire datasets, was associated with periapical granuloma samples. Consistently with literature, the anaerobic taxa detected were most abundant in radicular cyst samples. Indeed, a higher abundance of presumptive predicted metabolic pathways related to Lipopolysaccharide biosynthesis was found in radicular cyst samples. CONCLUSIONS: The present pilot study confirmed the different microbial characterization of the two main apical periodontitis types and shade light on the possible role of L. lactis in periapical granulomas.


Assuntos
Microbiota/genética , Periodontite Periapical/microbiologia , RNA Ribossômico 16S/genética , Adulto , Feminino , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Humanos , Masculino , Granuloma Periapical/microbiologia , Projetos Piloto , Cisto Radicular/microbiologia , Adulto Jovem
18.
Int J Mol Sci ; 19(2)2018 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-29425177

RESUMO

The rapid development and application of nanotechnology to biological interfaces has impacted the bone implant field, allowing researchers to finely modulate the interface between biomaterials and recipient tissues. In the present study, oxidative anodization was exploited to generate two alumina surfaces with different pore diameters. The former displayed surface pores in the mean range of 16-30 nm, while in the latter pores varied from to 65 to 89 nm. The samples were characterized by Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray spectroscopy (EDX) analysis prior to being tested with pre-osteoblastic MC3T3-E1 cells. In vitro cell response was studied in terms of early cell adhesion, viability, and morphology, including focal adhesion quantification. Both the alumina samples promoted higher cell adhesion and viability than the control condition represented by the standard culture dish plastic. Osteogenic differentiation was assessed through alkaline phosphatase activity and extracellular calcium deposition, and it was found that of the two nano-surfaces, one was more efficient than the other. By comparing for the first time two nano-porous alumina surfaces with different pore diameters, our data supported the role of nano-topography in inducing cell response. Modulating a simple aspect of surface texture may become an attractive route for guiding bone healing and regeneration around implantable metals.


Assuntos
Óxido de Alumínio/química , Nanoporos , Osteoblastos/efeitos dos fármacos , Tecidos Suporte/química , Animais , Adesão Celular , Técnicas de Cultura de Células/métodos , Diferenciação Celular , Linhagem Celular , Camundongos , Osteoblastos/citologia , Osteoblastos/fisiologia , Tecidos Suporte/efeitos adversos
20.
Cell Death Differ ; 25(3): 573-588, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29229999

RESUMO

Heme is required for cell respiration and survival. Nevertheless, its intracellular levels need to be finely regulated to avoid heme excess, which may catalyze the production of reactive oxygen species (ROS) and promote cell death. Here, we show that alteration of heme homeostasis in endothelial cells due to the loss of the heme exporter FLVCR1a, results in impaired angiogenesis. In vitro, FLVCR1a silencing in endothelial cells causes defective tubulogenesis and poor viability due to intracellular heme accumulation. Consistently, endothelial-specific Flvcr1a knockout mice show aberrant angiogenesis responsible for hemorrhages and embryonic lethality. Importantly, we demonstrate that impaired heme export leads to endothelial cell death by paraptosis and provide evidence that endoplasmic reticulum (ER) stress precedes heme-induced paraptosis. These findings highlight a crucial role for the cytosolic heme pool in the control of endothelial cell survival and in the regulation of the angiogenic process. Interfering with endothelial heme export represents a valuable model for a deeper understanding of the molecular mechanisms underlying heme-triggered paraptosis and, in the future, might provide a novel tool for the modulation of angiogenesis in pathophysiologic conditions.


Assuntos
Apoptose , Células Endoteliais/metabolismo , Heme/metabolismo , Proteínas de Membrana Transportadoras/metabolismo , Neovascularização Patológica/metabolismo , Receptores Virais/metabolismo , Animais , Apoptose/genética , Células Cultivadas , Estresse do Retículo Endoplasmático/genética , Feminino , Heme/genética , Humanos , Masculino , Proteínas de Membrana Transportadoras/deficiência , Proteínas de Membrana Transportadoras/genética , Camundongos , Camundongos Knockout , Neovascularização Patológica/genética , Neovascularização Patológica/patologia , Receptores Virais/deficiência , Receptores Virais/genética
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