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1.
FEMS Microbiol Ecol ; 96(1)2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31769782

RESUMO

Bacteria frequently cooperate by sharing secreted metabolites such as enzymes and siderophores. The expression of such 'public good' traits can be interdependent, and studies on laboratory systems have shown that trait linkage affects eco-evolutionary dynamics within bacterial communities. Here, we examine whether linkage among social traits occurs in natural habitats by examining investment levels and correlations between five public goods (biosurfactants, biofilm components, proteases, pyoverdines and toxic compounds) in 315 Pseudomonas isolates from soil and freshwater communities. Our phenotypic assays revealed that (i) social trait expression profiles varied dramatically; (ii) correlations between traits were frequent, exclusively positive and sometimes habitat-specific; and (iii) heterogeneous (specialised) trait repertoires were rarer than homogeneous (unspecialised) repertoires. Our results show that most isolates lie on a continuum between a 'social' type producing multiple public goods, and an 'asocial' type showing low investment into social traits. This segregation could reflect local adaptation to different microhabitats, or emerge from interactions between different social strategies. In the latter case, our findings suggest that the scope for competition among unspecialised isolates exceeds the scope for mutualistic exchange of different public goods between specialised isolates. Overall, our results indicate that complex interdependencies among social traits shape microbial lifestyles in nature.

2.
Biomolecules ; 9(12)2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31801266

RESUMO

Segmentation is one of the most important stages in the 3D reconstruction of macromolecule structures in cryo-electron microscopy. Due to the variability of macromolecules and the low signal-to-noise ratio of the structures present, there is no generally satisfactory solution to this process. This work proposes a new unsupervised particle picking and segmentation algorithm based on the composition of two well-known image filters: Anisotropic (Perona-Malik) diffusion and non-negative matrix factorization. This study focused on keyhole limpet hemocyanin (KLH) macromolecules which offer both a top view and a side view. Our proposal was able to detect both types of views and separate them automatically. In our experiments, we used 30 images from the KLH dataset of 680 positive classified regions. The true positive rate was 95.1% for top views and 77.8% for side views. The false negative rate was 14.3%. Although the false positive rate was high at 21.8%, it can be lowered with a supervised classification technique.

3.
Sensors (Basel) ; 20(1)2019 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-31877787

RESUMO

Horizontal displacements of a multiple-anchor pile wall in a 28.5 m deep excavation using the top-down construction method have been monitored using optical fiber (Brillouin optical time-domain reflectometry (BOTDR)), strain gauges, inclinometers, and a topographic survey. This work presents a comparison between these different techniques to measure horizontal displacements in the pile at several stages of the soil excavation process. It was observed that displacements can be separated into two components: Rigid body motion and pile flexural deformation. Measurements using optical fiber and inclinometers are considered the most adequate and easy to install. A numerical model allows us to evaluate the influence of earth pressure on the estimated horizontal displacements. It is shown that using soil pressure on the wall given by p = 0.65Kaγh, on a simplified modeled wall, provides a close deduction of horizontal displacements compared to observed values on the field.

4.
Span J Psychol ; 22: E59, 2019 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-31868159

RESUMO

The main aims of this study were, first to analyze the partial effects of specific mechanisms of moral disengagement (MMD) on different manifestations of general aggression and dating violence (DV) in adolescents and youths; second, to explore the moderating effects of gender and age on these relations. Moral disengagement, and different forms of aggression and DV, were evaluated in a sample of 424 participants (61.1% women) aged 15 to 25 years (M = 18.80; SD = 2.69). Pearson correlations and different hierarchical multiple linear regression analyses were conducted. All regressions were controlled by social desirability. MMD contributed to physical aggression with a medium effect size (R2 = .22) and verbal aggression with a small effect size (R2 = .10). Conversely, MMD did not appear to clearly contribute to DV. In conclusion, depersonalization and rationalization were the most important MMD for physical aggression, rationalization for verbal aggression, and irresponsibility for verbal-emotional violence in dating relationships. Moreover, the relationships between physical aggression and rationalization mechanism were significantly moderated by gender: there appears to be a stronger relationship between rationalization and physical aggression in boys than in girls. Moderating effects of age on these relations were not found.

5.
J Sports Sci ; 37(23): 2653-2659, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31419921

RESUMO

This study assessed the intra-individual reliability of oxygen saturation in intercostal muscles (SmO2-m.intercostales) during an incremental maximal treadmill exercise by using portable NIRS devices in a test-retest study. Fifteen marathon runners (age, 24.9 ± 2.0 years; body mass index, 21.6 ± 2.3 kg·m-2; V̇O2-peak, 63.7 ± 5.9 mL·kg-1·min-1) were tested on two separate days, with a 7-day interval between the two measurements. Oxygen consumption (V̇O2) was assessed using the breath-by-breath method during the V̇O2-test, while SmO2 was determined using a portable commercial device, based in the near-infrared spectroscopy (NIRS) principle. The minute ventilation (VE), respiratory rate (RR), and tidal volume (Vt) were also monitored during the cardiopulmonary exercise test. For the SmO2-m.intercostales, the intraclass correlation coefficient (ICC) at rest, first (VT1) and second ventilatory (VT2) thresholds, and maximal stages were 0.90, 0.84, 0.92, and 0.93, respectively; the confidence intervals ranged from -10.8% - +9.5% to -15.3% - +12.5%. The reliability was good at low intensity (rest and VT1) and excellent at high intensity (VT2 and max). The Spearman correlation test revealed (p ≤ 0.001) an inverse association of SmO2-m.intercostales with V̇O2 (ρ = -0.64), VE (ρ = -0.73), RR (ρ = -0.70), and Vt (ρ = -0.63). The relationship with the ventilatory variables showed that increased breathing effort during exercise could be registered adequately using a NIRS portable device.


Assuntos
Exercício Físico/fisiologia , Músculos Intercostais/fisiologia , Consumo de Oxigênio/fisiologia , Espectroscopia de Luz Próxima ao Infravermelho/instrumentação , Trabalho Respiratório/fisiologia , Adulto , Desempenho Atlético/fisiologia , Teste de Esforço/métodos , Humanos , Masculino , Reprodutibilidade dos Testes , Taxa Respiratória/fisiologia , Corrida/fisiologia , Volume de Ventilação Pulmonar/fisiologia , Adulto Jovem
6.
J Exp Clin Cancer Res ; 38(1): 234, 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31159852

RESUMO

BACKGROUND: Ovarian cancer is the leading cause of gynecologic cancer-related death, due in part to a late diagnosis and a high rate of recurrence. Primary and acquired platinum resistance is related to a low response probability to subsequent lines of treatment and to a poor survival. Therefore, a comprehensive understanding of the mechanisms that drive platinum resistance is urgently needed. METHODS: We used bioinformatics analysis of public databases and RT-qPCR to quantitate the relative gene expression profiles of ovarian tumors. Many of the dysregulated genes were cancer stem cell (CSC) factors, and we analyzed its relation to therapeutic resistance in human primary tumors. We also performed clustering and in vitro analyses of therapy cytotoxicity in tumorspheres. RESULTS: Using bioinformatics analysis, we identified transcriptional targets that are common endpoints of genetic alterations linked to platinum resistance in ovarian tumors. Most of these genes are grouped into 4 main clusters related to the CSC phenotype, including the DNA damage, Notch and C-KIT/MAPK/MEK pathways. The relative expression of these genes, either alone or in combination, is related to prognosis and provide a connection between platinum resistance and the CSC phenotype. However, the expression of the CSC-related markers was heterogeneous in the resistant tumors, most likely because there were different CSC pools. Furthermore, our in vitro results showed that the inhibition of the CSC-related targets lying at the intersection of the DNA damage, Notch and C-KIT/MAPK/MEK pathways sensitize CSC-enriched tumorspheres to platinum therapies, suggesting a new option for the treatment of patients with platinum-resistant ovarian cancer. CONCLUSIONS: The current study presents a new approach to target the physiology of resistant ovarian tumor cells through the identification of core biomarkers. We hypothesize that the identified mutations confer platinum resistance by converging to activate a few pathways and to induce the expression of a few common, measurable and targetable essential genes. These pathways include the DNA damage, Notch and C-KIT/MAPK/MEK pathways. Finally, the combined inhibition of one of these pathways with platinum treatment increases the sensitivity of CSC-enriched tumorspheres to low doses of platinum, suggesting a new treatment for ovarian cancer.


Assuntos
Antineoplásicos/farmacologia , Biomarcadores Tumorais , Resistencia a Medicamentos Antineoplásicos/genética , Células-Tronco Neoplásicas/efeitos dos fármacos , Células-Tronco Neoplásicas/metabolismo , Neoplasias Ovarianas/genética , Platina/farmacologia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Tomada de Decisão Clínica , Gerenciamento Clínico , Feminino , Humanos , Estimativa de Kaplan-Meier , Técnicas de Diagnóstico Molecular , Neoplasias Ovarianas/diagnóstico , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Ovarianas/mortalidade , Prognóstico , Modelos de Riscos Proporcionais , Resultado do Tratamento
7.
PLoS One ; 14(4): e0215325, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30973954

RESUMO

The differential contribution of maternal and paternal acceptance-rejection to children's psychological adjustment has been explained by differences in interpersonal power and prestige within families; however, there is not yet enough empirical support for this explanation. This study examines the moderating effects of interpersonal power and prestige on the relationship between perceived parental acceptance-rejection and psychological adjustment across children's sex and age. The sample was composed of 913 children ranging in age from 9 to 16 years. Multiple hierarchical regression analyses in the total sample showed a significant and independent contribution of parental acceptance-rejection and parental power and prestige. No moderating effects of interpersonal power and prestige were found for the total sample. However, when the regression analyses were conducted across different age groups, maternal acceptance had a higher contribution to psychological adjustment in children from nine to ten years old. Interestingly, the moderating effects of interpersonal prestige (not interpersonal power) were also significant in younger participants. Furthermore, the moderating effects of prestige on maternal acceptance-rejection were different in late childhood than in early adolescence. These results suggest how parental prestige may explain the higher contribution of maternal acceptance to younger children's psychological adjustment.


Assuntos
Ajustamento Emocional , Relações Pais-Filho , Adaptação Psicológica , Adolescente , Fatores Etários , Criança , Pai/psicologia , Feminino , Humanos , Masculino , Modelos Psicológicos , Mães/psicologia , Espanha , Inquéritos e Questionários
8.
Span. j. psychol ; 22: e59.1-e59.10, 2019. tab
Artigo em Inglês | IBECS-Express | ID: ibc-ET5-776

RESUMO

The main aims of this study were, first to analyze the partial effects of specific mechanisms of moral disengagement (MMD) on different manifestations of general aggression and dating violence (DV) in adolescents and youths; second, to explore the moderating effects of gender and age on these relations. Moral disengagement, and different forms of aggression and DV, were evaluated in a sample of 424 participants (61.1% women) aged 15 to 25 years (M = 18.80; SD = 2.69). Pearson correlations and different hierarchical multiple linear regression analyses were conducted. All regressions were controlled by social desirability. MMD contributed to physical aggression with a medium effect size (R2 = .22) and verbal aggression with a small effect size (R2 = .10). Conversely, MMD did not appear to clearly contribute to DV. In conclusion, depersonalization and rationalization were the most important MMD for physical aggression, rationalization for verbal aggression, and irresponsibility for verbal-emotional violence in dating relationships. Moreover, the relationships between physical aggression and rationalization mechanism were significantly moderated by gender: there appears to be a stronger relationship between rationalization and physical aggression in boys than in girls. Moderating effects of age on these relations were not found


No disponible

9.
Psicol. conduct ; 26(3): 495-512, sept.-dic. 2018. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-176048

RESUMO

El presente estudio explora la dimensionalidad de la "Escala de depresión del Centro de Estudios Epidemiológicos para niños y adolescentes" (CES-DC; Radloff, 1977, 1991) en una muestra de 986 niños y adolescentes españoles (55% chicas) con edades entre los 9 y 18 anos. Se realizo un análisis factorial confirmatorio para un modelo de cuatro factores de primer orden basado en la estructura original (Afecto depresivo, Somatización, Afecto positivo y Problemas interpersonales), así como para otro modelo alternativo de segundo orden que agrega los factores previos. Se examinaron las propiedades psicométricas por items, por dimensiones y para la escala total. También se valoro la validez de criterio con diferentes variables que teóricamente se relacionan con la sintomatología depresiva. Los resultados muestran que los modelos explorados son adecuados para la población infantojuvenil española con pruebas de validez de criterio y propiedades psicométricas satisfactorias. Se discute el potencial discriminativo y las cualidades de la escala para la evaluación de la sintomatología depresiva en niños y adolescentes


The present study explores the dimensionality of the Center for Epidemiological Studies Depression Scale for Children and Adolescents (CES-DC; Radloff, 1977, 1991) in a sample of 986 Spanish children and adolescents (55% girls) between 9 and 18 years old. Confirmatory factor analysis was performed for a first order 4-factor model based on the original structure (Depressed affect, Somatic, Positive affect, and Interpersonal difficulties) and for another alternative model of second order which includes the previous factors. We examined the psychometric properties by items, by dimensions and for the total scale. The criterion validity was also evaluated with different variables which are theoretically related to the depressive symptomatology. The results show that the tested models are adequate for Spanish children and adolescents. Furthermore, evidence of criterion validity and psychometric properties were satisfactory. The potential discriminant validity and quality of the scale to assess the children's depressive symptoms are discussed


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Comportamento do Adolescente/psicologia , Estresse Psicológico/psicologia , Depressão/epidemiologia , Psicometria/métodos , Comportamento Infantil/psicologia , Psicologia do Adolescente/estatística & dados numéricos , Análise Fatorial
10.
Heliyon ; 4(3): e00574, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29862340

RESUMO

Advances in eye-tracking technology have led to better human-computer interaction, and involve controlling a computer without any kind of physical contact. This research describes the transformation of a commercial eye-tracker for use as an alternative peripheral device in human-computer interactions, implementing a pointer that only needs the eye movements of a user facing a computer screen, thus replacing the need to control the software by hand movements. The experiment was performed with 30 test individuals who used the prototype with a set of educational videogames. The results show that, although most of the test subjects would prefer a mouse to control the pointer, the prototype tested has an empirical precision similar to that of the mouse, either when trying to control its movements or when attempting to click on a point of the screen.

11.
Span J Psychol ; 21: E20, 2018 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-29880070

RESUMO

The aim of this study is to provide an overview of the development of premature children, including attachment, child psychological adjustment and parental variables. 130 children < 1,500 g or < 32 weeks at birth from two public hospitals, assessed at two years corrected age, together with their parents. Parental socio-demographic data was collected. Infant development, attachment and child psychological adjustment were evaluated, as was parental stress. The percentage of preterm children with developmental delays ranged from 5% to 21%. Girls tend to show higher levels of development than boys with effect sizes ranging from small, η2p = .02, to medium, η2p = .07. Secure attachment was the most frequent pattern in the sample. No significant differences, p < .05, between preterm children and the normative population were found on children´s behavioral problems and maternal stress levels. Despite the fact prematurity is considered to be a risk factor for a child´s development, a significant proportion of these children do not show problems in terms of developmental levels, attachment pattern and maternal stress. However, socio-emotional and affective domains, as well as psychological support programs for parenthood, should be followed up from a multidisciplinary perspective.


Assuntos
Adaptação Psicológica/fisiologia , Desenvolvimento Infantil/fisiologia , Deficiências do Desenvolvimento/fisiopatologia , Recém-Nascido Prematuro/psicologia , Mães/psicologia , Apego ao Objeto , Poder Familiar/psicologia , Estresse Psicológico/psicologia , Pré-Escolar , Feminino , Humanos , Masculino , Espanha
12.
Span. j. psychol ; 21: e20.1-e20.12, 2018. tab
Artigo em Inglês | IBECS-Express | ID: ibc-ET1-5405

RESUMO

The aim of this study is to provide an overview of the development of premature children, including attachment, child psychological adjustment and parental variables. 130 children < 1,500 g or < 32 weeks at birth from two public hospitals, assessed at two years corrected age, together with their parents. Parental socio-demographic data was collected. Infant development, attachment and child psychological adjustment were evaluated, as was parental stress. The percentage of preterm children with developmental delays ranged from 5% to 21%. Girls tend to show higher levels of development than boys with effect sizes ranging from small, η2p = .02, to medium, η2p = .07. Secure attachment was the most frequent pattern in the sample. No significant differences, p < .05, between preterm children and the normative population were found on children's behavioral problems and maternal stress levels. Despite the fact prematurity is considered to be a risk factor for a child's development, a significant proportion of these children do not show problems in terms of developmental levels, attachment pattern and maternal stress. However, socio-emotional and affective domains, as well as psychological support programs for parenthood, should be followed up from a multidisciplinary perspective


No disponible

14.
Indian J Orthop ; 51(5): 487-492, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28966371

RESUMO

BACKGROUND: Tear of the anterior cruciate ligament (ACL) is a common ligamentous injury of the knee. Reconstruction of this ligament is often required to restore functional stability of the knee. Outcome of ACL reconstruction is significantly affected by how the graft is fixed to the bone. This study is to determine if there is a different clinical outcome after cortical versus cortical-cancellous suspension femoral fixation in hamstring based anterior cruciate ligament (ACL) reconstruction. MATERIALS AND METHODS: This is a retrospective comparative study conducted between 2006 and 2010. We enrolled patients who underwent arthroscopic ACL reconstruction. Sixty two patients met inclusion criteria and 41 agreed to come for followup assessment. Median age was of 28 years (range 18-39 years). Demographic baseline profile of both groups was similar. The femoral fixation devices were cortical (n = 16) and cortical-cancellous suspension techniques (n = 25). The average period of evolution at the time of assessment was 40 months (range 12-72 months). The patients were examined according to Lachman test (using Rolimeter knee tester), anterior drawer test, pivot shift test, International Knee Documentation Committee questionnaire, and Tegner-Lysholm knee scoring scale. RESULTS: The objective evaluation of the patients (Lachman test) showed better results in terms of stability in the group of patients who underwent the cortical-cancellous suspension method. These differences were not reflected in the assessment of activity level (Tegner-Lysholm), where both groups showed the same results. CONCLUSIONS: ACL reconstruction with both cortical and cortical-cancellous suspension femoral fixation techniques show the same clinical results at medium long followup. However, cortical-cancellous fixations seem to provide greater stability to the reconstruction.

15.
An. psicol ; 33(3): 605-611, oct. 2017. graf, tab
Artigo em Inglês | IBECS | ID: ibc-165635

RESUMO

The two fold aim of this study was first, to analyze the effects of anticipatory fatigue, emotional symptomatology and belonging to a clinical group on the physical and cognitive perception of fatigue, and second, to explore the potential moderating effect of anticipatory fatigue on the relationship between symptomatology or clinical condition and perceived fatigue. The conditional and partial effects of independent variables were analyzed by hierarchical regression in an ex-post-facto correlational design. The sample was composed of 317 participants (29% from a clinical population). Anticipatory fatigue (by an ad hoc scale), and perception of fatigue (by the Chalder Fatigue Scale) were measured. Emotional symptoms were assessed by Goldberg’s GHQ-28 questionnaire. Anticipatory fatigue and emotional symptoms (mainly depressive) had significant effects on cognitive and physical fatigue. Belonging to the clinical group significantly and exclusively predicted cognitive fatigue. Furthermore, anticipatory fatigue moderated between-group effects (clinical versus general) and cognitive fatigue. In brief, emotional symptoms (mainly depressive) and anticipatory fatigue significantly predicted perceived cognitive and physical fatigue. Anticipation of fatigue moderated the effect of clinical group on cognitive fatigue after controlling for depressive symptomatology (AU)


El objetivo de este estudio fue doble, primero, analizar los efectos de la fatiga anticipatoria, la sintomatología emocional y la pertenencia a un grupo clínico sobre la percepción de fatiga física y mental; segundo, explorar el potencial efecto moderador de la anticipación de la fatiga en las relaciones entre la sintomatología o la condición clínica y la sensación percibida de fatiga. Se analiza mediante un diseño ex post facto y correlacional los efectos parciales y condicionados de las variables predictivas mediante diferentes análisis de regresión jerárquica. Participaron 317 sujetos (29% procedentes de población clínica). Se evaluó la fatiga anticipatoria (Escala elaborada ad hoc), la experiencia percibida de fatiga (Escala de Fatiga de Chalder et al., 1993), y la sintomatología emocional (GHQ-28 de Goldberg, 1996). Los resultados mostraron efectos significativos de la fatiga anticipatoria y la sintomatología emocional, predominantemente de la sintomatología depresiva sobre la percepción de fatiga física y mental. La pertenencia a un grupo clínico predecía de forma significativa y exclusiva la fatiga cognitiva. Además, la fatiga anticipatoria moderaba el efecto del grupo (clínico versus general) sobre la fatiga cognitiva. En conclusión, la sintomatología, principalmente la depresiva, y la fatiga anticipatoria, tienen un valor predictivo significativo en la experiencia percibida de la fatiga física y mental. La anticipación de fatiga moderaba el efecto del grupo clínico sobre la experiencia de fatiga cognitiva una vez controlada la sintomatología depresiva (AU)


Assuntos
Humanos , Antecipação Psicológica , Fadiga/psicologia , Sintomas Afetivos/psicologia , Ansiedade/psicologia , Depressão/psicologia , Transtornos Somatoformes/psicologia
16.
Pap. psicol ; 38(2): 135-147, mayo-ago. 2017. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-164407

RESUMO

Se presenta una revisión sistemática de estudios sobre prevalencia de la violencia en el noviazgo (física, psicológica y sexual) cometida y sufrida en adolescentes y jóvenes. De 1221 referencias iniciales, 113 cumplían con los criterios de calidad preestablecidos (evaluación de la violencia con instrumentos fiables y válidos, muestras superiores a 500 participantes u obtenidas mediante muestreo probabilístico). Se encontró una gran variabilidad en los datos de prevalencia, con rangos de variación entre el 3.8% y el 41.9% en violencia física cometida; entre el 0.4% y el 57.3% en violencia física sufrida; entre el 4.2% y el 97% en violencia psicológica cometida; entre el 8.5% y el 95.5% en violencia psicológica sufrida; entre el 1.2% y el 58.8% en violencia sexual cometida; y entre el 0.1% y el 64.6% en violencia sexual sufrida. Los resultados sugieren una mayor prevalencia en la perpetración de agresiones psicológicas por mujeres y sexuales por los hombres; una mayor victimización psicológica y sexual en mujeres; así como tasas ligeramente superiores de agresiones en los adolescentes que en los adultos jóvenes. Asimismo, en muchos de los trabajos revisados las agresiones tenían una naturaleza bidireccional (AU)


This article conducts a systematic review on the prevalence of dating violence in adolescents and young people. Primary studies about physical, psychological and sexual dating violence (perpetration and victimization) were analyzed. A total of 1,221 references were found and, out of those, 113 met the pre-established quality criteria (studies had to have used assessment tools with evidence of reliability and validity in samples of over 500 participants or obtained through probabilistic sampling). The results showed great variability in the figures on the prevalence of dating violence. Specifically, the percentages range from 3.8% to 41.9% for perpetrated physical violence; from 0.4% to 57.3% for victimized physical violence; from 4.2% to 97% for perpetrated psychological violence; from 8.5% to 95.5% for victimized psychological violence; from 1.2% to 58.8% for perpetrated sexual violence; and finally, from 0.1% to 64.6% for victimized sexual violence. The results suggest a higher prevalence in the perpetration of psychological aggression by women and sexual violence by men; greater psychological and sexual victimization in women; and slightly higher rates of aggression in adolescents than in young adults. Also, in many of the papers the aggression had a bidirectional nature (AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Violência contra a Mulher , Violência por Parceiro Íntimo/psicologia , Comportamento do Adolescente/psicologia , Delitos Sexuais , Parceiros Sexuais/psicologia , Vítimas de Crime/psicologia , Prevalência , Fatores de Risco
17.
Psychiatry Res ; 256: 88-95, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28624678

RESUMO

This study analyzes how a child's clinical condition modifies the relationship of father involvement, parental (fathers and mothers) acceptance and the child´s psychological adjustment, including the internalizing and externalizing problems. The cohort studied was composed of 226 subjects (61.94% males) with a mean age of 14.08 years, of which 113 children were from an incidental clinical sample and 113 from the general population. Both groups were matched by sex, age and family status, and the data show that the same structure of parent-child relationships that predict the child´s psychological adjustment can be accepted for both the clinical and non-clinical groups of children. However, the intensity of the relationships between the variables father involvement, parental acceptance and the child´s outcomes differed in function of the child's clinical status. Specifically, in the clinically-referred sample compared to non-clinical sample father involvement had a stronger effect on the children´s internalizing (but not the externalizing) problems and on their global psychological adjustment via their perceived maternal acceptance. The role of father involvement in the family relationships of clinical children is discussed. This study provides important evidence to support the need for enhanced paternal participation in the intervention programs for families.


Assuntos
Adaptação Psicológica , Comportamento do Adolescente/psicologia , Comportamento Infantil/psicologia , Ajustamento Emocional , Relações Pai-Filho , Pai/psicologia , Adaptação Psicológica/fisiologia , Adolescente , Criança , Estudos de Coortes , Ajustamento Emocional/fisiologia , Feminino , Humanos , Masculino , Mães/psicologia , Pais/psicologia , Autorrelato , Adulto Jovem
18.
BMC Cell Biol ; 18(1): 21, 2017 04 21.
Artigo em Inglês | MEDLINE | ID: mdl-28427322

RESUMO

BACKGROUND: Biomaterials are widely used to regenerate or substitute bone tissue. In order to evaluate their potential use for clinical applications, these need to be tested and evaluated in vitro with cell culture models. Frequently, immortalized osteoblastic cell lines are used in these studies. However, their uncontrolled proliferation rate, phenotypic changes or aberrations in mitotic processes limits their use in long-term investigations. Recently, we described a new pluripotent-like subpopulation of dental pulp stem cells derived from the third molars (DPPSC) that shows genetic stability and shares some pluripotent characteristics with embryonic stem cells. In this study we aim to describe the use of DPPSC to test biomaterials, since we believe that the biomaterial cues will be more critical in order to enhance the differentiation of pluripotent stem cells. METHODS: The capacity of DPPSC to differentiate into osteogenic lineage was compared with human sarcoma osteogenic cell line (SAOS-2). Collagen and titanium were used to assess the cell behavior in commonly used biomaterials. The analyses were performed by flow cytometry, alkaline phosphatase and mineralization stains, RT-PCR, immunohistochemistry, scanning electron microscopy, Western blot and enzymatic activity. Moreover, the genetic stability was evaluated and compared before and after differentiation by short-comparative genomic hybridization (sCGH). RESULTS: DPPSC showed excellent differentiation into osteogenic lineages expressing bone-related markers similar to SAOS-2. When cells were cultured on biomaterials, DPPSC showed higher initial adhesion levels. Nevertheless, their osteogenic differentiation showed similar trend among both cell types. Interestingly, only DPPSC maintained a normal chromosomal dosage before and after differentiation on 2D monolayer and on biomaterials. CONCLUSIONS: Taken together, these results promote the use of DPPSC as a new pluripotent-like cell model to evaluate the biocompatibility and the differentiation capacity of biomaterials used in bone regeneration.


Assuntos
Técnicas de Cultura de Células/métodos , Instabilidade Cromossômica/fisiologia , Polpa Dentária/citologia , Teste de Materiais/métodos , Dente Serotino/citologia , Osteogênese/fisiologia , Células-Tronco Pluripotentes/citologia , Adolescente , Materiais Biocompatíveis , Diferenciação Celular , Linhagem Celular Tumoral , Células Cultivadas , Hibridização Genômica Comparativa , Feminino , Humanos , Masculino , Dente Serotino/metabolismo , Células-Tronco Pluripotentes/metabolismo , Células-Tronco Pluripotentes/fisiologia , Engenharia Tecidual , Adulto Jovem
19.
Clin Oral Implants Res ; 28(7): 840-848, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27335267

RESUMO

OBJECTIVES: The aims of this randomized clinical trial were to compare the dimensional changes and the histological composition after using deproteinized bovine bone mineral (DBBM) or deproteinized bovine bone mineral with 10% collagen (DBBM-C) and a collagen membrane in ridge preservation procedures. MATERIAL AND METHODS: Patients who required an extraction and a subsequent implant-supported rehabilitation at a non-molar site were recruited. After extraction, a cone beam computed tomography (CBCT) was performed and sites were randomly treated either with DBBM or DBBM-C plus a collagen membrane. At 5 months, before implant placement, a second CBCT was performed and a biopsy of the area was obtained. A blinded investigator superimposed the CBCTs and performed measurements to determine bone volume changes between the two time points. Additionally, a histomorphometric analysis of the biopsies was performed in a blinded manner. RESULTS: Eleven sites belonged to the DBBM group and eleven to the DBBM-C group. All together, a significant reduction in height and width was observed at 5 months of healing, but no statistically significant differences were observed between the DBBM and the DBBM-C group. The histomorphometric analysis revealed a similar composition in terms of newly formed bone, connective tissue and residual graft particles in both groups. CONCLUSIONS: Deproteinized bovine bone mineral with 10% collagen showed a similar behaviour as DBBM not only in its capacity to minimize ridge contraction but also from a histological point of view. Thus, both graft materials seem to be suitable for ridge preservation procedures.


Assuntos
Perda do Osso Alveolar/diagnóstico por imagem , Perda do Osso Alveolar/prevenção & controle , Aumento do Rebordo Alveolar/métodos , Colágeno/uso terapêutico , Tomografia Computadorizada de Feixe Cônico , Minerais/uso terapêutico , Animais , Biópsia , Bovinos , Implantação Dentária Endo-Óssea , Implantes Dentários , Prótese Dentária Fixada por Implante , Método Duplo-Cego , Feminino , Humanos , Masculino , Mandíbula/cirurgia , Pessoa de Meia-Idade , Espanha , Retalhos Cirúrgicos , Suínos , Extração Dentária , Alvéolo Dental/cirurgia
20.
HPB Surg ; 2016: 4614096, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27803512

RESUMO

Objective. To evaluate if early cholecystectomy (EC) is the most appropriate treatment for acute cholecystitis compared to delayed cholecystectomy (DC). Patients and Methods. A retrospective cohort study of 1043 patients was carried out, with a group of 531 EC cases and a group of 512 DC patients. The following parameters were recorded: (1) postoperative hospital morbidity, (2) hospital mortality, (3) days of hospital stay, (4) readmissions, (5) admission to the Intensive Care Unit (ICU), (6) type of surgery, (7) operating time, and (8) reoperations. In addition, we estimated the direct cost savings of implementing an EC program. Results. The overall morbidity of the EC group (29.9%) was significantly lower than the DC group (38.7%). EC demonstrated significantly better results than DC in days of hospital stay (8.9 versus 15.8 days), readmission percentage (6.8% versus 21.9%), and percentage of ICU admission (2.3% versus 7.8%), which can result in reducing the direct costs. The patients who benefited most from an EC were those with a Charlson index > 3. Conclusions. EC is safe in patients with acute cholecystitis and could lead to a reduction in the direct costs of treatment.

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