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1.
Int. j. morphol ; 38(3): 523-529, June 2020. graf
Artigo em Inglês | LILACS | ID: biblio-1098282

RESUMO

This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the ischemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothermia and ketoprofen is involved.


Este estudio tuvo como objetivo investigar la morfometría y el patrón de expresión de proteínas y genes relacionados con la vía apoptótica extrínseca en la isquemia cerebral focal experimental y el agujero de neuroprotección con hipotermia y ketoprofeno. Se dividieron aleatoriamente 120 ratas en 3 grupos (20 animales cada uno): control - sin cirugía (20 animales); simulación - simulación de cirugía (20 animales); isquemia isquemia focal durante 1 hora, sin reperfusión (80 animales) y dividida en cuatro subgrupos con 20 animales cada uno: isquemia + hipotermia intraisquémica; isquemia + ketoprofeno intravenoso previo, e isquemia + hipotermia y ketoprofeno. El volumen del infarto se midió utilizando un análisis morfométrico de áreas de infarto definidas por cloruro de trifenil tetrazolio y los patrones de expresión de los genes de apoptosis (Fas, c-Flip, caspase-8 y caspase-3) y la proteína de apoptosis caspase-3 fueron evaluados por PCR cuantitativa en tiempo real e inmunohistoquímica, respectivamente. Se observó hipoexpresión de genes de la vía extrínseca de la apoptosis: receptor Fas, c-Flip y caspasa-8 en las áreas isquémicas. También se observaron aumentos en el gen y la proteína caspasa-3 en las áreas isquémicas y estos aumentos se redujeron por hipotermia y ketoprofeno, también observado por estudio morfométrico. El aumento de caspasas-3 sugiere que este gen tiene un papel importante en la apoptosis, y probable causa de muerte celular, involucrando el efecto neuroprotector de la hipotermia y el ketoprofeno.


Assuntos
Animais , Ratos , Isquemia Encefálica/genética , Isquemia Encefálica/metabolismo , Imuno-Histoquímica , Isquemia Encefálica/patologia , Isquemia Encefálica/terapia , Cetoprofeno/farmacologia , Apoptose/genética , Fármacos Neuroprotetores/farmacologia , Modelos Animais de Doenças , Caspase 3/genética , Caspase 8/genética , Reação em Cadeia da Polimerase em Tempo Real , Hipotermia Induzida
2.
J Sci Food Agric ; 99(2): 666-674, 2019 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-29962086

RESUMO

BACKGROUND: Zinc (Zn) deficiency is a major human health concern worldwide, and biofortification (genetic and agronomic) is a complementary solution for increasing micronutrient contents, including Zn. Cassava (Manihot esculenta Crantz) has been used for Zn biofortification because it is an important staple crop in most countries affected by malnutrition and Zn deficiency. Thus studies on biofortification of this crop can improve its nutritional quality. Zn content in cassava clones enriched with ß-carotene or lycopene and cultivated under different areas and soil managements was investigated to evaluate the influence of genotypic variation and agronomic management on Zn status in the plant. RESULTS: A clone-specific response to total Zn content in the soil was found, with clones 26, 215, and 240 (ß-carotene enriched) and clones 341 and 395 (lycopene enriched) being the most responsive. For both experiments, there was a positive interaction between total soil Zn and Zn content in the roots. CONCLUSIONS: Our results suggest that, by combining plant breeding and agronomic strategies, it is possible to enrich cassava roots with both zinc and ß-carotene or lycopene. © 2018 Society of Chemical Industry.


Assuntos
Biofortificação/métodos , Licopeno/análise , Manihot/química , Zinco/análise , beta Caroteno/análise , Alimentos Fortificados/análise , Licopeno/metabolismo , Manihot/metabolismo , Raízes de Plantas/química , Raízes de Plantas/metabolismo , Solo/química , Zinco/metabolismo , beta Caroteno/metabolismo
3.
Free Radic Biol Med ; 115: 421-435, 2018 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-29248721

RESUMO

In this study, we report the ability of a set of eight 3-phenylcoumarin derivatives bearing 6,7- or 5,7-dihydroxyl groups, free or acetylated, bound to the benzopyrone moiety, to modulate the effector functions of human neutrophils. In general, (i) 6,7-disubstituted compounds (5, 6, 19, 20) downmodulated the Fcγ receptor-mediated neutrophil oxidative metabolism more strongly than 5,7-disubstituted compounds (21, 22, 23, 24), and (ii) hydroxylated compounds (5, 19, 21, 23) downmodulated this neutrophil function more effectively than their acetylated counterparts (6, 20, 22, 24, respectively). Compounds 5 (6,7-dihydroxy-3-[3',4'-methylenedioxyphenyl]-coumarin) and 19 (6,7-dihydroxy-3-[3',4'-dihydroxyphenyl]-coumarin) effectively downmodulated the neutrophil oxidative metabolism elicited via Fcγ and/or complement receptors. Compound 5 also downmodulated the immune complex-stimulated phagocytosis, degranulation of elastase, and production and release of neutrophil extracellular traps, as well as the human neutrophil chemotaxis towards n-formyl-methionyl-leucyl-phenylalanine, without altering the expression level of formyl peptide receptor type 1. Both compounds 5 and 19 did not impair the neutrophil capacity to recognize and kill Candida albicans. Docking calculations revealed that compounds 5 and 19 directly interacted with three catalytic residues - Gln-91, His-95, and Arg-239 - inside the myeloperoxidase active site. Together, these findings indicate that (i) inhibition of reactive oxygen species generation and degranulation of elastase are closely associated with downmodulation of release of neutrophil extracellular traps; and (ii) compound 5 can be a prototype for the development of novel immunomodulating drugs to treat immune complex-mediated inflammatory diseases.


Assuntos
Anti-Inflamatórios/farmacologia , Cumarínicos/farmacologia , Armadilhas Extracelulares/metabolismo , Neutrófilos/fisiologia , Elastase Pancreática/metabolismo , Receptores de Complemento/metabolismo , Receptores de IgG/metabolismo , Anti-Inflamatórios/química , Células Cultivadas , Cumarínicos/química , Humanos , Imunomodulação , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Peroxidase/metabolismo , Fagocitose , Espécies Reativas de Oxigênio/metabolismo
4.
Arq Neuropsiquiatr ; 75(1): 30-35, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28099560

RESUMO

Objective:: To evaluate the microRNA-219 and NMDA expression in brain tissue and blood of animals subjected to cerebral ischemia associated with alcoholism. Methods:: Fifty Wistar rats were divided into groups: control, sham, ischemic, alcoholic, and ischemic plus alcoholic. The expression of microRNA-219 and NMDA were analyzed by real-time PCR. Results:: When compared to the control group, the microRNA-219 in brain tissue was less expressed in the ischemic, alcoholic, and ischemic plus alcoholic groups. In the blood, this microRNA had lower expression in alcoholic and ischemic plus alcoholic groups. In the brain tissue the NMDA gene expression was greater in the ischemic, alcoholic, and ischemic plus alcoholic groups. Conclusion:: A possible modulation of NMDA by microRNA-219 was observed with an inverse correlation between them.


Assuntos
Alcoolismo/complicações , Isquemia Encefálica/metabolismo , MicroRNAs/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Animais , Isquemia Encefálica/etiologia , Modelos Animais de Doenças , Imuno-Histoquímica , Masculino , Ratos , Ratos Wistar
5.
Arq. neuropsiquiatr ; 75(1): 30-35, Jan. 2017. graf
Artigo em Inglês | LILACS | ID: biblio-838854

RESUMO

ABSTRACT Alcohol consumption aggravates injuries caused by ischemia. Many molecular mechanisms are involved in the pathophysiology of cerebral ischemia, including neurotransmitter expression, which is regulated by microRNAs. Objective: To evaluate the microRNA-219 and NMDA expression in brain tissue and blood of animals subjected to cerebral ischemia associated with alcoholism. Methods: Fifty Wistar rats were divided into groups: control, sham, ischemic, alcoholic, and ischemic plus alcoholic. The expression of microRNA-219 and NMDA were analyzed by real-time PCR. Results: When compared to the control group, the microRNA-219 in brain tissue was less expressed in the ischemic, alcoholic, and ischemic plus alcoholic groups. In the blood, this microRNA had lower expression in alcoholic and ischemic plus alcoholic groups. In the brain tissue the NMDA gene expression was greater in the ischemic, alcoholic, and ischemic plus alcoholic groups. Conclusion: A possible modulation of NMDA by microRNA-219 was observed with an inverse correlation between them.


RESUMO Algumas condições podem agravar os danos causados pelo processo isquêmico, tais como o consumo de álcool, e diversos mecanismos moleculares que estão envolvidos na fisiopatologia da isquemia cerebral, incluindo a expressão de neurotransmissores, e estes podem estar regulados por microRNAs. Objetivo: Avaliar a expressão de NMDA e do microRNA-219 no tecido cerebral e no sangue de animais submetidos à isquemia cerebral associada ao alcoolismo. Métodos: 50 ratos Wistar foram divididos em: controle, sham, isquêmico, alcoólico e isquêmico mais alcoólico. A expressão de microRNA-219 e de NMDA foram analisadas por PCR em tempo real. Resultados: Quando comparado com o grupo controle, o microRNA-219 no tecido cerebral foi menos expresso nos grupos isquêmico, alcoólico e associado. No sangue, este microRNA teve menor expressão no grupo alcoólico e no associado. Em relação à expressão do gene do NMDA, em tecido cerebral foi maior nos grupos isquêmico, alcoólico e no associado. Conclusão: Uma possível modulação de NMDA pelo microRNA-219 foi observada, com uma correlação inversa entre eles.


Assuntos
Animais , Masculino , Ratos , Isquemia Encefálica/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , MicroRNAs/metabolismo , Alcoolismo/complicações , Imuno-Histoquímica , Isquemia Encefálica/etiologia , Ratos Wistar , Modelos Animais de Doenças
7.
Acta Odontol Latinoam ; 26(3): 161-6, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-25335369

RESUMO

The masticatory process identifies the level of the individual's masticatory ability and provides important information for an adequate diagnosis of the masticatory function. The purpose of this cross-sectional study was to evaluate the influence of age on habitual and non-habitual mastication by means of the values of the ensemble average of masticatory cycles. All volunteers were Brazilian, Caucasian, fully dentate (except for Group I--mixed dentition), aged 7-80 years and divided into five groups: I (7-12 years), II (13-20 years), III (21- 40 years), IV (41-60 years) and V (61-80 years). Except for Group V, which comprised nine women and eight men, all groups were equally divided with respect to gender (20 M/20 F). All subjects were nasal breathers, had normal occlusion and no parafunctional habits or symptoms of temporomandibular dysfunction (RDC/TMD). The masticatory process was analyzed during habitual mastication of peanuts and raisins, and non-habitual mastication of ParafilmM, for 10 seconds each. The resulting electromyographic data were evaluated using SPSS 19.0 software for Windows. ANOVA (analysis of variance) followed by the Duncan test were used to compare the efficiency of the masticatory cycle between age groups. Multivariate analysis (General Linear Models) was used to analyze the effect of age groups and gender on the efficiency of themasticatory cycle, to compare groups (p < 0.05). The results showed that age is directly associated with the changes in masticatory process in healthy subjects.


Assuntos
Envelhecimento/fisiologia , Mastigação/fisiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Estudos Transversais , Eletromiografia/métodos , Feminino , Humanos , Masculino , Músculo Masseter/fisiologia , Pessoa de Meia-Idade , Contração Muscular/fisiologia , Fatores Sexuais , Músculo Temporal/fisiologia , Adulto Jovem
8.
Acta Odontol Latinoam ; 26(3): 161-6, 2013.
Artigo em Espanhol | BINACIS, BINACIS | ID: bin-132705

RESUMO

The masticatory process identifies the level of the individuals masticatory ability and provides important information for an adequate diagnosis of the masticatory function. The purpose of this cross-sectional study was to evaluate the influence of age on habitual and non-habitual mastication by means of the values of the ensemble average of masticatory cycles. All volunteers were Brazilian, Caucasian, fully dentate (except for Group I--mixed dentition), aged 7-80 years and divided into five groups: I (7-12 years), II (13-20 years), III (21- 40 years), IV (41-60 years) and V (61-80 years). Except for Group V, which comprised nine women and eight men, all groups were equally divided with respect to gender (20 M/20 F). All subjects were nasal breathers, had normal occlusion and no parafunctional habits or symptoms of temporomandibular dysfunction (RDC/TMD). The masticatory process was analyzed during habitual mastication of peanuts and raisins, and non-habitual mastication of ParafilmM, for 10 seconds each. The resulting electromyographic data were evaluated using SPSS 19.0 software for Windows. ANOVA (analysis of variance) followed by the Duncan test were used to compare the efficiency of the masticatory cycle between age groups. Multivariate analysis (General Linear Models) was used to analyze the effect of age groups and gender on the efficiency of themasticatory cycle, to compare groups (p < 0.05). The results showed that age is directly associated with the changes in masticatory process in healthy subjects.


Assuntos
Envelhecimento/fisiologia , Mastigação/fisiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Estudos Transversais , Eletromiografia/métodos , Feminino , Humanos , Masculino , Músculo Masseter/fisiologia , Pessoa de Meia-Idade , Contração Muscular/fisiologia , Fatores Sexuais , Músculo Temporal/fisiologia , Adulto Jovem
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