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1.
Acta Cir Bras ; 33(9): 853-861, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30328918

RESUMO

PURPOSE: To evaluate a novel and adapted low-cost set model for laparoscopic surgery in rats. METHODS: Nine Wistar rats underwent two different laparoscopic procedures, splenectomy (n=3) and distal pancreatectomy with splenectomy (n = 6), after assembling a low-cost set replacing the conventional one (monitor, micro camera, image processor, light source, laparoscope and insufflator). The new set included an Android Tablet 10.5 ", a 5mm USB Endoscope and semiautomatic sphygmomanometer monitor. RESULTS: The same surgeon performed the laparoscopic procedures. Total surgical time ranged from 36 to 60 minutes with a mean of 45.8 minutes. Three rats died during the distal pancreatic and splenectomy procedure (33.3%), due to respiratory failure (n = 1), uncontrolled abdominal hemorrhage (n=1) and iatrogenic gastric perforation (n = 1). We followed the other six rats (66.6%) for seven days with no further evidence of complications. CONCLUSIONS: The laparoscopic partial pancreatectomy and splenectomy can be performed with the novel low-cost set assembled in the present experimental study. Both specific training and skills development are required to validate more advanced laparoscopic procedures and achieve a desirable outcome.


Assuntos
Laparoscopia/educação , Pancreatectomia/educação , Esplenectomia/educação , Animais , Custos e Análise de Custo , Laparoscopia/economia , Laparoscopia/métodos , Modelos Animais , Pancreatectomia/economia , Pancreatectomia/métodos , Ratos , Ratos Wistar , Esplenectomia/economia , Esplenectomia/métodos
2.
Acta cir. bras ; 33(9): 853-861, Sept. 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-973494

RESUMO

Abstract Purpose: To evaluate a novel and adapted low-cost set model for laparoscopic surgery in rats. Methods: Nine Wistar rats underwent two different laparoscopic procedures, splenectomy (n=3) and distal pancreatectomy with splenectomy (n = 6), after assembling a low-cost set replacing the conventional one (monitor, micro camera, image processor, light source, laparoscope and insufflator). The new set included an Android Tablet 10.5 ", a 5mm USB Endoscope and semiautomatic sphygmomanometer monitor. Results: The same surgeon performed the laparoscopic procedures. Total surgical time ranged from 36 to 60 minutes with a mean of 45.8 minutes. Three rats died during the distal pancreatic and splenectomy procedure (33.3%), due to respiratory failure (n = 1), uncontrolled abdominal hemorrhage (n=1) and iatrogenic gastric perforation (n = 1). We followed the other six rats (66.6%) for seven days with no further evidence of complications. Conclusions: The laparoscopic partial pancreatectomy and splenectomy can be performed with the novel low-cost set assembled in the present experimental study. Both specific training and skills development are required to validate more advanced laparoscopic procedures and achieve a desirable outcome.

3.
Sci Rep ; 7(1): 16273, 2017 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-29176736

RESUMO

Ribosomal proteins (RPs) play a fundamental role within all type of cells, as they are major components of ribosomes, which are essential for translation of mRNAs. Furthermore, these proteins are involved in various physiological and pathological processes. The intrinsic biological relevance of RPs motivated advanced studies for the identification of unrevealed RPs. In this work, we propose a new computational method, termed Rama, for the prediction of RPs, based on machine learning techniques, with a particular interest in plants. To perform an effective classification, Rama uses a set of fundamental attributes of the amino acid side chains and applies a two-step procedure to classify proteins with unknown function as RPs. The evaluation of the resultant predictive models showed that Rama could achieve mean sensitivity, precision, and specificity of 0.91, 0.91, and 0.82, respectively. Furthermore, a list of proteins that have no annotation in Phytozome v.10, and are annotated as RPs in Phytozome v.12, were correctly classified by our models. Additional computational experiments have also shown that Rama presents high accuracy to differentiate ribosomal proteins from RNA-binding proteins. Finally, two novel proteins of Arabidopsis thaliana were validated in biological experiments. Rama is freely available at http://inctipp.bioagro.ufv.br:8080/Rama .

4.
BMC Bioinformatics ; 18(1): 431, 2017 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-28964254

RESUMO

BACKGROUND: Geminiviruses infect a broad range of cultivated and non-cultivated plants, causing significant economic losses worldwide. The studies of the diversity of species, taxonomy, mechanisms of evolution, geographic distribution, and mechanisms of interaction of these pathogens with the host have greatly increased in recent years. Furthermore, the use of rolling circle amplification (RCA) and advanced metagenomics approaches have enabled the elucidation of viromes and the identification of many viral agents in a large number of plant species. As a result, determining the nomenclature and taxonomically classifying geminiviruses turned into complex tasks. In addition, the gene responsible for viral replication (particularly, the viruses belonging to the genus Mastrevirus) may be spliced due to the use of the transcriptional/splicing machinery in the host cells. However, the current tools have limitations concerning the identification of introns. RESULTS: This study proposes a new method, designated Fangorn Forest (F2), based on machine learning approaches to classify genera using an ab initio approach, i.e., using only the genomic sequence, as well as to predict and classify genes in the family Geminiviridae. In this investigation, nine genera of the family Geminiviridae and their related satellite DNAs were selected. We obtained two training sets, one for genus classification, containing attributes extracted from the complete genome of geminiviruses, while the other was made up to classify geminivirus genes, containing attributes extracted from ORFs taken from the complete genomes cited above. Three ML algorithms were applied on those datasets to build the predictive models: support vector machines, using the sequential minimal optimization training approach, random forest (RF), and multilayer perceptron. RF demonstrated a very high predictive power, achieving 0.966, 0.964, and 0.995 of precision, recall, and area under the curve (AUC), respectively, for genus classification. For gene classification, RF could reach 0.983, 0.983, and 0.998 of precision, recall, and AUC, respectively. CONCLUSIONS: Therefore, Fangorn Forest is proven to be an efficient method for classifying genera of the family Geminiviridae with high precision and effective gene prediction and classification. The method is freely accessible at www.geminivirus.org:8080/geminivirusdw/discoveryGeminivirus.jsp .


Assuntos
Geminiviridae/genética , Aprendizado de Máquina , Área Sob a Curva , DNA Satélite/classificação , DNA Satélite/genética , Geminiviridae/classificação , Internet , Fases de Leitura Aberta/genética , Plantas/virologia , Curva ROC , Interface Usuário-Computador
5.
BMC Bioinformatics ; 18(1): 240, 2017 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-28476106

RESUMO

BACKGROUND: The Geminiviridae family encompasses a group of single-stranded DNA viruses with twinned and quasi-isometric virions, which infect a wide range of dicotyledonous and monocotyledonous plants and are responsible for significant economic losses worldwide. Geminiviruses are divided into nine genera, according to their insect vector, host range, genome organization, and phylogeny reconstruction. Using rolling-circle amplification approaches along with high-throughput sequencing technologies, thousands of full-length geminivirus and satellite genome sequences were amplified and have become available in public databases. As a consequence, many important challenges have emerged, namely, how to classify, store, and analyze massive datasets as well as how to extract information or new knowledge. Data mining approaches, mainly supported by machine learning (ML) techniques, are a natural means for high-throughput data analysis in the context of genomics, transcriptomics, proteomics, and metabolomics. RESULTS: Here, we describe the development of a data warehouse enriched with ML approaches, designated geminivirus.org. We implemented search modules, bioinformatics tools, and ML methods to retrieve high precision information, demarcate species, and create classifiers for genera and open reading frames (ORFs) of geminivirus genomes. CONCLUSIONS: The use of data mining techniques such as ETL (Extract, Transform, Load) to feed our database, as well as algorithms based on machine learning for knowledge extraction, allowed us to obtain a database with quality data and suitable tools for bioinformatics analysis. The Geminivirus Data Warehouse (geminivirus.org) offers a simple and user-friendly environment for information retrieval and knowledge discovery related to geminiviruses.


Assuntos
Biologia Computacional/métodos , Bases de Dados Genéticas , Geminiviridae/genética , Aprendizado de Máquina , Algoritmos , DNA de Cadeia Simples/genética , DNA Viral/genética , Fases de Leitura Aberta/genética , Filogenia , Plantas/virologia
6.
Clin Exp Med ; 15(3): 311-20, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24934325

RESUMO

Acute pancreatitis (AP) is an inflammatory disorder that can affect adjacent and/or remote organs. Some evidence indicates that the production of reactive oxygen species is able to induce AP. Protein carbonyl (PC) derivatives, which can also be generated through oxidative cleavage mechanisms, have been implicated in several diseases, but there is little or no information on this biomarker in AP. We investigated the association between some inflammatory mediators and PC, with the severity of ischemia-reperfusion AP. Wistar rats (n = 56) were randomly assigned in the following groups : control; sham, 15- or 180-min clamping of splenic artery, with 24 or 72 h of follow-up. The relationships between serum level of PC and thiobarbituric acid reactive species (TBARS) to myeloperoxidase (MPO) activity in tissue homogenates and to cytokines in culture supernatants of pancreatic samples were analyzed. MPO activity was related to the histology scores and increased in all clamping groups. Tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1ß), and interleukin-6 were higher in the 180-min groups. Significant correlations were found between MPO activity and the concentrations of TNF-α and IL-1ß. PC levels increased in the 15-min to 24-h group. TBARS levels were not altered substantially. MPO activity and TNF-α and IL-1ß concentrations in pancreatic tissue are correlated with AP severity. Serum levels of PC appear to begin to rise early in the course of the ischemia-reperfusion AP and are no longer detected at later stages in the absence of severe pancreatitis. These data suggest that PC can be an efficient tool for the diagnosis of early stages of AP.


Assuntos
Biomarcadores/análise , Pancreatite Necrosante Aguda/diagnóstico , Pancreatite Necrosante Aguda/patologia , Carbonilação Proteica , Traumatismo por Reperfusão/patologia , Animais , Citocinas/análise , Modelos Animais de Doenças , Feminino , Peroxidase/análise , Ratos Wistar
7.
aSEPHallus ; 7(14): 27-42, mai.-out. 2012.
Artigo em Português | Index Psicologia - Periódicos técnico-científicos | ID: psi-60266

RESUMO

No trabalho clínico com pacientes toxicômanos, somos colocados constantemente diante de impasses que comprometem o progresso do tratamento, como, por exemplo, a indiferença frente aos riscos de morte, pouquíssima aderência ao tratamento e a prevalência do ato em detrimento às palavras. Para além de tais manifestações, escolhemos, como objeto deste artigo, a relação autoerótica do sujeito toxicômano com o corpo, tomando-a como paradigma da estrutura viciante própria da pulsão. Assim, o crescente número de casos de adições compulsivas, especificamente as toxicomanias, apresenta-se em nossa contemporaneidade marcado pela permissividade ao gozo desmedido como um paradigma do caráter entorpecente e extasiante próprio da pulsão(AU)


In clinical work with addicted patients, many impasses arise that might compromise the progress of treatment, for example, the indifference to the risk of death, very little treatment compliance and preference for act instead of the words. Beyond these manifestations, we chose as the subject of this article, the autoerotic relation of the junkie subject with its body, taking it as a paradigm of the addictive structure which is characteristic of drive itself. Thus, the growing number of cases of compulsive addictions, specifically to drugs presents itself in our contemporary world marked by excessive permissiveness to enjoyment as a paradigm of the numbing and exhilarating character of drive(AU)


Dans le travail clinique avec les patients toxicomanes, nous sommes placés en permanence devant les impasses qui compromettent les progrès du traitement, par exemple, l'indifférence au risque de la mort, très peu d’observance du traitement et la prévalence de l’acte en detriment des mots. Au delà de ces manifestations, nous avons choisi comme sujet de cet article, la relation de l'auto-érotique du junkie sujet avec le corps, en le prenant comme un paradigme de la structure addictive de la pulsion elle même. Ainsi, le nombre croissant de cas d'additions compulsives, toxicomanies spécifiquement, présent dans notre monde contemporain marqué par la permissivité excessive à la jouissance démesurée comme un paradigme Du charactère engourdissant et extasiant propre de la pulsion(AU)


Assuntos
Psicanálise , Transtornos Relacionados ao Uso de Substâncias , Narcisismo
8.
aSEPHallus ; 7(14): 27-42, maio-out. 2012.
Artigo em Português | LILACS | ID: lil-723497

RESUMO

No trabalho clínico com pacientes toxicômanos, somos colocados constantemente diante de impasses que comprometem o progresso do tratamento, como, por exemplo, a indiferença frente aos riscos de morte, pouquíssima aderência ao tratamento e a prevalência do ato em detrimento às palavras. Para além de tais manifestações, escolhemos, como objeto deste artigo, a relação autoerótica do sujeito toxicômano com o corpo, tomando-a como paradigma da estrutura viciante própria da pulsão. Assim, o crescente número de casos de adições compulsivas, especificamente as toxicomanias, apresenta-se em nossa contemporaneidade marcado pela permissividade ao gozo desmedido como um paradigma do caráter entorpecente e extasiante próprio da pulsão.


In clinical work with addicted patients, many impasses arise that might compromise the progress of treatment, for example, the indifference to the risk of death, very little treatment compliance and preference for act instead of the words. Beyond these manifestations, we chose as the subject of this article, the autoerotic relation of the junkie subject with its body, taking it as a paradigm of the addictive structure which is characteristic of drive itself. Thus, the growing number of cases of compulsive addictions, specifically to drugs presents itself in our contemporary world marked by excessive permissiveness to enjoyment as a paradigm of the numbing and exhilarating character of drive.


Dans le travail clinique avec les patients toxicomanes, nous sommes placés en permanence devant les impasses qui compromettent les progrès du traitement, par exemple, l'indifférence au risque de la mort, très peu d’observance du traitement et la prévalence de l’acte en detriment des mots. Au delà de ces manifestations, nous avons choisi comme sujet de cet article, la relation de l'auto-érotique du junkie sujet avec le corps, en le prenant comme un paradigme de la structure addictive de la pulsion elle même. Ainsi, le nombre croissant de cas d'additions compulsives, toxicomanies spécifiquement, présent dans notre monde contemporain marqué par la permissivité excessive à la jouissance démesurée comme un paradigme Du charactère engourdissant et extasiant propre de la pulsion.


Assuntos
Narcisismo , Psicanálise , Transtornos Relacionados ao Uso de Substâncias
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