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1.
J Contemp Dent Pract ; 22(3): 268-272, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34210927

RESUMO

AIM: To evaluate the active tactile sensitivity in individuals with complete natural dentition, determining the smallest thickness detected by the participants, and clarifying if there is a difference between the thicknesses analyzed. MATERIALS AND METHODS: Active tactile sensitivity was evaluated in 40 research participants. Inclusion criteria included participants with complete natural dentition, without active or history of periodontal disease, absence of temporomandibular disorders, bruxism, and restorations in the evaluated area. Exclusion criteria included age below 18 years. The active tactile perception threshold was evaluated by using carbon sheets of different thicknesses (0, 12, 24, 40, 80, 100, and 200 µm), which were inserted in the participants' premolars, bilaterally. The carbon sheet was inserted so as not to come into contact with the oral soft tissues. Subsequently, the participant occluded and was asked about the perception of the intraocclusal object 20 times in each occlusal contact. The collected data were tabulated considering the amount of positive and negative responses for each carbon thickness. Values of p < 0.05 were considered significant. RESULTS: The results showed that there was linearity in perception, on both sides, besides, the natural dentition was able to perceive difference in thickness from 12 µm. CONCLUSION: We conclude that the 12 µm thickness is noticeable in occlusion and can be differentiated from other thicknesses in natural dentition and that there is no difference between the tactile sensitivity of the right and left sides. CLINICAL SIGNIFICANCE: A better understanding of active oral tactile sensitivity will contribute to numerous clinical applications in dentistry, including occlusal adjustment in dental rehabilitation, dental implants prosthesis design, and survival of prosthetic rehabilitation.


Assuntos
Bruxismo , Boca Edêntula , Adolescente , Oclusão Dentária , Dentição , Humanos , Tato
2.
Mol Genet Genomic Med ; 6(5): 689-701, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30078197

RESUMO

Dentistry constitutes the basic nucleus of professionals of higher level of health in Brazil with one of the largest concentrations of dentists per capita in the world. However, the genetic in dentistry in Brazil is explored, basically, in research field. Future actions need to be performed in order to deep the whole knowledge about diagnosis and treatment of diseases with genetic basis in dentistry, in Brazil.


Assuntos
Genética Médica , Doenças Dentárias/genética , Brasil , Humanos
3.
PLoS One ; 10(12): e0143068, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26630491

RESUMO

Aquaporins (AQP) are water channel proteins and the genes coding for AQP2, AQP5, and AQP6 are clustered in 12q13. Since AQP5 is expressed in serous acinar cells of salivary glands, we investigated its involvement in caries. DNA samples from 1,383 individuals from six groups were studied. Genotypes of eight single nucleotide polymorphisms covering the aquaporin locus were tested for association with caries experience. Interaction with genes involved in enamel formation was tested. The association between enamel microhardness at baseline, after creation of artificial caries lesion, and after exposure to fluoride and the genetic markers in AQP5 was tested. Finally, AQP5 expression in human whole saliva, after exposure to fluoride in a mammary gland cell line, which is known to express AQP5, and in Wistar rats was also verified. Nominal associations were found between caries experience and markers in the AQP5 locus. Since these associations suggested that AQP5 may be inhibited by levels of fluoride in the drinking water that cause fluorosis, we showed that fluoride levels above optimal levels change AQP5 expression in humans, cell lines, and rats. We have shown that AQP5 is involved in the pathogenesis of caries and likely interacts with fluoride.


Assuntos
Aquaporina 5/metabolismo , Cárie Dentária/metabolismo , Fluoretos/metabolismo , Adolescente , Adulto , Animais , Aquaporina 5/genética , Linhagem Celular Tumoral , Criança , Pré-Escolar , Cárie Dentária/genética , Feminino , Marcadores Genéticos/genética , Genótipo , Humanos , Masculino , Glândulas Mamárias Humanas/metabolismo , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único/genética , Ratos , Ratos Wistar , Saliva/metabolismo , Adulto Jovem
4.
BMC Oral Health ; 15: 33, 2015 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-25887438

RESUMO

BACKGROUND: Peri-implantitis is a chronic inflammation, resulting in loss of supporting bone around implants. Chronic periodontitis is a risk indicator for implant failure. Both diseases have a common etiology regarding inflammatory destructive response. BRINP3 gene is associated with aggressive periodontitis. However, is still unclear if chronic periodontitis and peri-implantitis have the same genetic background. The aim of this work was to investigate the association between BRINP3 genetic variation (rs1342913 and rs1935881) and expression and susceptibility to both diseases. METHODS: Periodontal and peri-implant examinations were performed in 215 subjects, divided into: healthy (without chronic periodontitis and peri-implantitis, n = 93); diseased (with chronic periodontitis and peri-implantitis, n = 52); chronic periodontitis only (n = 36), and peri-implantitis only (n = 34). A replication sample of 92 subjects who lost implants and 185 subjects successfully treated with implants were tested. DNA was extracted from buccal cells. Two genetic markers of BRINP3 (rs1342913 and rs1935881) were genotyped using TaqMan chemistry. Chi-square (p < 0.05) compared genotype and allele frequency between groups. A subset of subjects (n = 31) had gingival biopsies harvested. The BRINP3 mRNA levels were studied by CT method (2(ΔΔCT)). Mann-Whitney test correlated the levels of BRINP3 in each group (p < 0.05). RESULTS: Statistically significant association between BRINP3 rs1342913 and peri-implantitis was found in both studied groups (p = 0.04). The levels of BRINP3 mRNA were significantly higher in diseased subjects compared to healthy individuals (p = 0.01). CONCLUSION: This study provides evidence that the BRINP3 polymorphic variant rs1342913 and low level of BRINP3 expression are associated with peri-implantitis, independently from the presence of chronic periodontitis.


Assuntos
Periodontite Crônica/genética , Proteínas de Ligação a DNA/genética , Peri-Implantite/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Estudos Transversais , Implantes Dentários , Índice de Placa Dentária , Planejamento de Prótese Dentária , Feminino , Regulação da Expressão Gênica/genética , Frequência do Gene/genética , Marcadores Genéticos/genética , Predisposição Genética para Doença/genética , Variação Genética/genética , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Osseointegração/fisiologia , Índice Periodontal , Polimorfismo de Nucleotídeo Único/genética
5.
Injury ; 45 Suppl 5: S7-S13, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25528626

RESUMO

The aim of this study was to assess the union rates in a series of patients with failed femoral shaft aseptic non-union who were treated with percutaneous concentrated autologous bone marrow grafting. Bone marrow harvesting and cell injection were performed under general anaesthesia in a single surgical procedure. Radiographic union was diagnosed in fractures with a score ≥ 10 according to the radiographic union scale in tibial fractures (RUST) and confirmed by clinical examination. Eight out of 16 patients progressed to consolidation (RUST score ≥ 10). Radiographic evidence of fracture union was observed at an average of 4.75 ± 1.75 months (range 3 to 8 months). All eight patients who did not progress to union within 12 months following the cell grafting procedure had a RUST score ≤ 10 (range 4 to 9). There were no differences in age, number of previous surgeries, duration of nonunion and preoperative RUST score between the patients that developed solid union and those with failed consolidation. However, a relationship between the number of osteoprogenitors injected and the rate of union was noted, 20.2 ± 8.6 × 10(8) versus 9.8 ± 4.3 × 10(8), p<0.005, between the patients with and without union, respectively. The efficacy of percutaneous autologous concentrated bone marrow grafting seems to be related to the number of osteoprogenitors available in the aspirates. Optimisation of the aspiration technique and concentration process is of paramount importance to increase the incidence of a successful outcome.


Assuntos
Transplante de Medula Óssea , Fraturas do Fêmur/cirurgia , Fixação Intramedular de Fraturas/estatística & dados numéricos , Consolidação da Fratura , Fraturas não Consolidadas/cirurgia , Adulto , Feminino , Fraturas do Fêmur/diagnóstico por imagem , Fraturas do Fêmur/fisiopatologia , Seguimentos , Fraturas não Consolidadas/diagnóstico por imagem , Fraturas não Consolidadas/fisiopatologia , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Osteogênese , Reoperação/estatística & dados numéricos , Tomografia Computadorizada por Raios X , Transplante Autólogo , Resultado do Tratamento
6.
BMC Oral Health ; 14: 84, 2014 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-25008200

RESUMO

BACKGROUND: There is evidence for a genetic contribution to chronic periodontitis. In this study, we conducted a genome wide association study among 866 participants of the University of Pittsburgh Dental Registry and DNA Repository, whose periodontal diagnosis ranged from healthy (N = 767) to severe chronic periodontitis (N = 99). METHODS: Genotypingi of over half-million single nucleotide polymorphisms was determined. Analyses were done twice, first in the complete dataset of all ethnicities, and second including only samples defined as self-reported Whites. From the top 100 results, twenty single nucleotide polymorphisms had consistent results in both analyses (borderline p-values ranging from 1E-05 to 1E-6) and were selected to be tested in two independent datasets derived from 1,460 individuals from Porto Alegre, and 359 from Rio de Janeiro, Brazil. Meta-analyses of the Single nucleotide polymorphisms showing a trend for association in the independent dataset were performed. RESULTS: The rs1477403 marker located on 16q22.3 showed suggestive association in the discovery phase and in the Porto Alegre dataset (p = 0.05). The meta-analysis suggested the less common allele decreases the risk of chronic periodontitis. CONCLUSIONS: Our data offer a clear hypothesis to be independently tested regarding the contribution of the 16q22.3 locus to chronic periodontitis.


Assuntos
Mapeamento Cromossômico , Cromossomos Humanos Par 16/genética , Periodontite Crônica/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Cromossomos Humanos Par 21/genética , Periodontite Crônica/etnologia , Complicações do Diabetes , Grupos Étnicos/genética , Grupo com Ancestrais do Continente Europeu/genética , Feminino , Frequência do Gene/genética , Marcadores Genéticos/genética , Predisposição Genética para Doença/genética , Variação Genética/genética , Estudo de Associação Genômica Ampla , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único/genética , Fatores de Risco , Fumar
7.
Implant Dent ; 17(2): 169-75, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18545048

RESUMO

This case report presents an analysis of the clinical, radiographic, and histological features of a peri-implant lesion around an implant placed immediately after extraction of a tooth with a periapical lesion. A 52-year-old man received an immediate implant (3.75 x 11.5 mm2) placed in the anterior region of the maxilla. Three years after implant placement, the patient presented with swelling in the anterior portion of the maxilla. Radiographic examination showed a well-circumscribed radiolucency around the implant. The implant and the lesion were removed and fixed in 10% buffered formalin and processed. Histological analysis showed 3 types of epithelium: respiratory, cuboidal, and non-keratinized stratified squamous. In the cyst wall peripheral nerves, arteries, veins, and chronic inflammation were present. The diagnosis was nasopalatine duct cyst. We concluded that the nasopalatine duct cyst can develop in association with dental implants. Clinically, the lesion is similar to the classical nasopalatine duct cyst. Histological analysis should be mandatory in all cases of peri-implant lesions and in all dental periapical lesions before immediate implant placement.


Assuntos
Implantação Dentária Endo-Óssea/métodos , Implantes Dentários para Um Único Dente/efeitos adversos , Falha de Restauração Dentária , Cistos Maxilomandibulares/etiologia , Doenças Maxilares/etiologia , Doenças Nasais/etiologia , Implantação Dentária Endo-Óssea/efeitos adversos , Humanos , Cistos Maxilomandibulares/cirurgia , Masculino , Doenças Maxilares/cirurgia , Pessoa de Meia-Idade , Palato Duro , Periodontite Periapical/complicações , Extração Dentária , Alvéolo Dental/cirurgia
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