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1.
Ann Surg Oncol ; 2020 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-32661848

RESUMO

INTRODUCTION: Most of the literature has only reported outcomes on robotic minor non-anatomical hepatectomy. This study was undertaken to analyze and examine the safety, feasibly, and perioperative outcomes of robotic major hepatectomy at our institution. METHODS: All patients undergoing robotic major hepatectomy were prospectively followed. Major hepatectomy was defined as a resection of 3 or more segments. Data are expressed as median (mean ± SD). RESULTS: A total of 170 consecutive patients underwent robotic hepatectomies, of which 100 were major resections involving at least 3 segments. The 100 patients were of median 62 (61 ± 13.0) years, and 46% were women. Median BMI was 29 (29 ± 5.9) kg/m2 and median ASA class was 3 (3 ± 0.5). Thirty percent of robotic major hepatectomies were for hepatocellular carcinoma, 28% were for metastatic adenocarcinoma, 9% were for cholangiocarcinoma, and 5% were for metastatic neuroendocrine tumor. Prep time (in the room until incision) was a median 58 min (62 ± 18.4), extraction time (incision until specimen extraction) was 124 min (146 ± 99.5), console time was 198 min (210 ± 123.9), closure time (extraction until dressing placement) was 109 min (131 ± 93.8), operative duration was 246 min (269 ± 123.2), and time under anesthesia was 330 min (353 ± 109.6). Estimated blood loss was 175 ml (249 ± 275.9) and length of stay was 4 days (5 ± 4.3). Seven patients experienced postoperative complications. Thirteen patients were readmitted within 30 days, and one patient died within 30 days. CONCLUSION: Application of the robotic platform to major hepatectomy is safe and feasible. Our early experience shows that this minimally invasive approach results in excellent short-term outcomes.

4.
Eur Radiol ; 2020 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-32405754

RESUMO

OBJECTIVES: Imaging assessment for the clinical management of femoroacetabular impingement (FAI) syndrome remains controversial because of a paucity of evidence-based guidance and notable variability in clinical practice, ultimately requiring expert consensus. The purpose of this agreement is to establish expert-based statements on FAI imaging, using formal techniques of consensus building. METHODS: A validated Delphi method and peer-reviewed literature were used to formally derive consensus among 30 panel members (21 musculoskeletal radiologists and 9 orthopaedic surgeons) from 13 countries. Forty-four questions were agreed on, and recent relevant seminal literature was circulated and classified in five major topics ('General issues', 'Parameters and reporting', 'Radiographic assessment', 'MRI' and 'Ultrasound') in order to produce answering statements. The level of evidence was noted for all statements, and panel members were asked to score their level of agreement with each statement (0 to 10) during iterative rounds. Either 'consensus', 'agreement' or 'no agreement' was achieved. RESULTS: Forty-seven statements were generated, and group consensus was reached for 45 (95.7%). Seventeen of these statements were selected as most important for dissemination in advance. There was no agreement for the two statements pertaining to 'Ultrasound'. CONCLUSION: Radiographic evaluation is the cornerstone of hip evaluation. An anteroposterior pelvis radiograph and a Dunn 45° view are recommended for the initial assessment of FAI although MRI with a dedicated protocol is the gold standard imaging technique in this setting. The resulting consensus can serve as a tool to reduce variability in clinical practices and guide further research for the clinical management of FAI. KEY POINTS: • FAI imaging literature is extensive although often of low level of evidence. • Radiographic evaluation with a reproducible technique is the cornerstone of hip imaging assessment. • MRI with a dedicated protocol is the gold standard imaging technique for FAI assessment.

5.
Biomolecules ; 10(3)2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32204429

RESUMO

Transmissible spongiform encephalopathies or prion diseases are rapidly progressive neurodegenerative diseases, the clinical manifestation of which can resemble other promptly evolving neurological maladies. Therefore, the unequivocal ante-mortem diagnosis is highly challenging and was only possible by histopathological and immunohistochemical analysis of the brain at necropsy. Although surrogate biomarkers of neurological damage have become invaluable to complement clinical data and provide more accurate diagnostics at early stages, other neurodegenerative diseases show similar alterations hindering the differential diagnosis. To solve that, the detection of the pathognomonic biomarker of disease, PrPSc, the aberrantly folded isoform of the prion protein, could be used. However, the amounts in easily accessible tissues or body fluids at pre-clinical or early clinical stages are extremely low for the standard detection methods. The solution comes from the recent development of in vitro prion propagation techniques, such as Protein Misfolding Cyclic Amplification (PMCA) and Real Time-Quaking Induced Conversion (RT-QuIC), which have been already applied to detect minute amounts of PrPSc in different matrixes and make early diagnosis of prion diseases feasible in a near future. Herein, the most relevant tissues and body fluids in which PrPSc has been detected in animals and humans are being reviewed, especially those in which cell-free prion propagation systems have been used with diagnostic purposes.

6.
Rev. esp. cardiol. (Ed. impr.) ; 73(2): 131-138, feb. 2020. tab, graf
Artigo em Espanhol | IBECS-Express | ID: ibc-ET5-820

RESUMO

INTRODUCCIÓN Y OBJETIVOS: Aunque la estenosis de la válvula pulmonar (EVP) se considera una cardiopatía congénita de bajo riesgo, se ha descrito la aparición de complicaciones y necesidad de reintervenir durante el seguimiento. Los objetivos del estudio fueron evaluar los resultados a largo plazo de la EVP reparada e identificar predictores de complicaciones cardiovasculares y reintervención. MÉTODOS: Se estudió a 158 pacientes adultos con EVP reparada (reparaciones practicadas de 1957 a 2010) con seguimiento activo en un centro terciario de referencia. RESULTADOS: Se sometió a cirugía a 95 pacientes (60%) y a valvuloplastia percutánea con balón a 63 (40%). Tras una mediana de seguimiento de 27 [20-33] años, la mayoría de los pacientes (n=134 [84,8%]) estaban en clase funcional I de la New York Heart Association, pero 61 (38.6%) requirieron reintervención, principalmente reemplazo de la valvular pulmonar (n=28 [17,7%]), y 19 (12%) presentaron al menos una complicación cardiovascular: 13 (8,2%), arritmias supraventriculares; 6 (3,8%), insuficiencia cardiaca; 5 (3,2%), accidente cerebrovascular; 1 (0,6%), muerte; 1 (0,6%) tromboembolia, y 1 (0,6%), arritmia ventricular. El análisis multivariante mostró que la edad en el momento de la reparación de la EVP (HR=1,08; IC95%, 1,04-1,12; p <0,001) y la presencia de cianosis antes de la reparación (HR=5,23; IC95%, 1,99-13,78; p = 0,001) fueron predictores independientes de complicaciones cardiovasculares. CONCLUSIONES: Se puede esperar un buen resultado a largo plazo tras de la reparación de la EVP, pero pueden aparecer complicaciones y necesidad de reintervenir. Una edad más avanzada y la presencia de cianosis en el momento de la reparación de la EVP son predictores de complicaciones cardiovasculares e identifican a una población que requiere un control más estricto


INTRODUCTION AND OBJECTIVES: Although pulmonary valve stenosis (PVS) is considered a low risk congenital heart disease, there have been reports of complications and the need for reintervention throughout follow-up. The aims of this study were to evaluate the long-term outcome of repaired PVS and to identify predictors of cardiovascular complications and reintervention. METHODS: We studied 158 adult patients with repaired PVS (repair procedures performed from 1957 to 2010) receiving active follow-up in a tertiary referral center. RESULTS: A total of 95 patients (60%) received surgical treatment, and 63 patients (40%) received percutaneous pulmonary balloon valvuloplasty. At the end of follow-up (27 years, IQR, 20-33 years), most patients (n=134, 84.8%) were in New York Heart Association functional class I, but 61 patients (38.6%) required a reintervention, mainly pulmonary valve replacement (17.7%, n=28), and 19 patients (12%) had at least one cardiovascular complication: 13 (8.2%) supraventricular arrhythmias, 6 (3.8%) heart failure, 5 (3.2%) stroke, 1 (0.6%) death, 1 (0.6%) thromboembolism, and 1 (0.6%) ventricular arrhythmia. Multivariate analysis showed that age at PVS repair (HR, 1.08; 95%CI, 1.04-1.12; P <.001) and the presence of cyanosis before PVS repair (HR, 5.23; 95%CI, 1.99-13.78; P=.001) were independent predictors for cardiovascular complications. CONCLUSIONS: Good long-term outcome can be expected after PVS repair, but complications and the need for reintervention may appear. Older age and the presence of cyanosis at PVS repair emerged as predictors of cardiovascular complications and identified a population that may merit stricter control

7.
FASEB J ; 34(3): 3969-3982, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31944411

RESUMO

Unlike other species, prion disease has never been described in dogs even though they were similarly exposed to the bovine spongiform encephalopathy (BSE) agent. This resistance prompted a thorough analysis of the canine PRNP gene and the presence of a negatively charged amino acid residue in position 163 was readily identified as potentially fundamental as it differed from all known susceptible species. In the present study, the first transgenic mouse model expressing dog prion protein (PrP) was generated and challenged intracerebrally with a panel of prion isolates, none of which could infect them. The brains of these mice were subjected to in vitro prion amplification and failed to find even minimal amounts of misfolded prions providing definitive experimental evidence that dogs are resistant to prion disease. Subsequently, a second transgenic model was generated in which aspartic acid in position 163 was substituted for asparagine (the most common in prion susceptible species) resulting in susceptibility to BSE-derived isolates. These findings strongly support the hypothesis that the amino acid residue at position 163 of canine cellular prion protein (PrPC ) is a major determinant of the exceptional resistance of the canidae family to prion infection and establish this as a promising therapeutic target for prion diseases.

8.
Rev Esp Cardiol (Engl Ed) ; 73(2): 131-138, 2020 Feb.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31130344

RESUMO

INTRODUCTION AND OBJECTIVES: Although pulmonary valve stenosis (PVS) is considered a low risk congenital heart disease, there have been reports of complications and the need for reintervention throughout follow-up. The aims of this study were to evaluate the long-term outcome of repaired PVS and to identify predictors of cardiovascular complications and reintervention. METHODS: We studied 158 adult patients with repaired PVS (repair procedures performed from 1957 to 2010) receiving active follow-up in a tertiary referral center. RESULTS: A total of 95 patients (60%) received surgical treatment, and 63 patients (40%) received percutaneous pulmonary balloon valvuloplasty. At the end of follow-up (27 years, IQR, 20-33 years), most patients (n=134, 84.8%) were in New York Heart Association functional class I, but 61 patients (38.6%) required a reintervention, mainly pulmonary valve replacement (17.7%, n=28), and 19 patients (12%) had at least one cardiovascular complication: 13 (8.2%) supraventricular arrhythmias, 6 (3.8%) heart failure, 5 (3.2%) stroke, 1 (0.6%) death, 1 (0.6%) thromboembolism, and 1 (0.6%) ventricular arrhythmia. Multivariate analysis showed that age at PVS repair (HR, 1.08; 95%CI, 1.04-1.12; P <.001) and the presence of cyanosis before PVS repair (HR, 5.23; 95%CI, 1.99-13.78; P=.001) were independent predictors for cardiovascular complications. CONCLUSIONS: Good long-term outcome can be expected after PVS repair, but complications and the need for reintervention may appear. Older age and the presence of cyanosis at PVS repair emerged as predictors of cardiovascular complications and identified a population that may merit stricter control.

9.
Med Hypotheses ; 136: 109475, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31812012

RESUMO

BACKGROUND: Minimally invasive mitral valve repair or replacement (MIMVR) approaches have been increasingly adopted for the treatment of mitral regurgitation, allowing a shorter recovery time and improving postoperative quality of life. However, inadequate positioning of the right mini thoracotomy access (working port) translates into suboptimal exposure, prolonged operative times and, potentially, reduction in the quality of mitral repair. At present, we are missing tools to further improve the positioning of the working port in order to ameliorate surgical exposure in a patient- specific fashion. METHODS AND EVALUATION OF THE HYPOTHESIS: We hypothesized that computation of relevant anatomical measurements from preoperative CT scans in patients undergoing MIMVR may provide patient-specific information in order to propose the surgical access that best fits to the patient's morphology. We hypothesized that this may systematize optimal mitral valve exposure, facilitating the procedure and potentially ameliorating the outcomes. We also hypothesized that preoperative simulation of the working port site and surgical instruments' insertion using a three-dimensional virtual model of the patient is feasible and may help in the customization of ports positioning. The hypothesis was evaluated by a multidisciplinary team including cardiac surgeons, experts in medical image processing and biomedical engineers. CT scans of 14 patients undergoing MIMVR were segmented to visualize 3D chest bones and heart structures meshes. The mitral valve annulus is pointed manually by the expert or extracted automatically when contrast-enhanced CT scan was available. The valve plane was then calculated and the optimal incision location analyzed according to a) the perpendicularity and b) the distance between the intercostal spaces and the valve plane. An angle-chart representation for the 4th, 5th and 6th intercostal spaces and a color map illustrating the distance between the skin and the mitral valve were created. We started the development of a simulation tool for preoperative planning using 3D Slicer software. CONCLUSIONS: Several patient-specific factors (including the orientation of the mitral valve plane and the morphology of the chest cage) may influence the performance of a MIMVR procedure, but they are not quantitatively considered in the current planning strategy. We suggest that the clinical results of MIMVR can be improved through preoperative virtual simulation and computer-assisted surgery (through determination of working port and surgical instruments insertion positioning). Further research is justified and the development of a software tool for clinical evaluation is warranted to verify the current hypothesis.

10.
Radiol Case Rep ; 14(12): 1473-1477, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31641395

RESUMO

Giant multilocular prostatic cystadenomas are rare benign prostatic tumor, usually presenting as a large pelvic mass, compressing the adjacent organs but with no clear aggressive features, frequently causing obstructive voiding symptoms. Nowadays, imaging plays an important role on the adequate characterization of these lesions, not only on depicting their different internal components, but also the relationship with the adjacent structures, therefore providing the best preoperative surgical planning. Here we present a case of a 62-year-old patient with recurrent obstructive voiding symptoms due to a giant multilocular prostatic cystadenomas, with histologically correlation, posteriorly excised.

11.
Am Surg ; 85(9): 978-984, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31638510

RESUMO

Only a small percentage of patients fail laparoscopic fundoplications undertaken for gastroesophageal reflux disease. But because many laparoscopic fundoplications have been undertaken, surgeons frequently encounter patients in need of "redo" operations. This study was undertaken to evaluate the robotic approach versus laparoendoscopic single-site (LESS) approach for redo fundoplications. With an Institutional Review Board approval, 64 patients undergoing LESS (n = 32) or robotic (n = 32) redo antireflux operations were prospectively followed up. Data are presented as median (mean + SD). For LESS versus robotic redo operations, the operative duration was 145 (143 ± 33.5) versus 196 (208 ± 76.7) minutes (P < 0.01), estimated blood loss was 50 (80 ± 92.1) versus 20 (43 ± 57.1) mL (P = 0.07), and length of stay was 1 (3 ± 5.4) versus 1 (2 ± 1.9) day (P = 0.57); 1 LESS operation was converted to "open." Operative duration was longer for men (P = 0.01). Postoperative complications were not more frequent after Nissen (n = 36) or Toupet (n = 28) fundoplication, regardless of the approach. When matched by BMI, operative duration was prolonged by a large Type I to IV hiatal hernia (P = 0.01). Symptoms improved dramatically and were similar with both approaches, and patient satisfaction was high. Robotic redo antireflux operations take longer than LESS operations. LESS and robotic redo antireflux operations are both safe and offer significant and similar amelioration of symptoms after failed fundoplications.


Assuntos
Fundoplicatura/métodos , Hérnia Hiatal/cirurgia , Laparoscopia/métodos , Reoperação , Procedimentos Cirúrgicos Robóticos/métodos , Idoso , Perda Sanguínea Cirúrgica , Conversão para Cirurgia Aberta , Feminino , Seguimentos , Fundoplicatura/efeitos adversos , Humanos , Laparoscopia/efeitos adversos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Satisfação do Paciente , Complicações Pós-Operatórias , Procedimentos Cirúrgicos Robóticos/efeitos adversos , Resultado do Tratamento
12.
PLoS Pathog ; 15(10): e1008117, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31644574

RESUMO

The resolution of the three-dimensional structure of infectious prions at the atomic level is pivotal to understand the pathobiology of Transmissible Spongiform Encephalopathies (TSE), but has been long hindered due to certain particularities of these proteinaceous pathogens. Difficulties related to their purification from brain homogenates of disease-affected animals were resolved almost a decade ago by the development of in vitro recombinant prion propagation systems giving rise to highly infectious recombinant prions. However, lack of knowledge about the molecular mechanisms of the misfolding event and the complexity of systems such as the Protein Misfolding Cyclic Amplification (PMCA), have limited generating the large amounts of homogeneous recombinant prion preparations required for high-resolution techniques such as solid state Nuclear Magnetic Resonance (ssNMR) imaging. Herein, we present a novel recombinant prion propagation system based on PMCA that substitutes sonication with shaking thereby allowing the production of unprecedented amounts of multi-labeled, infectious recombinant prions. The use of specific cofactors, such as dextran sulfate, limit the structural heterogeneity of the in vitro propagated prions and makes possible, for the first time, the generation of infectious and likely homogeneous samples in sufficient quantities for studies with high-resolution structural techniques as demonstrated by the preliminary ssNMR spectrum presented here. Overall, we consider that this new method named Protein Misfolding Shaking Amplification (PMSA), opens new avenues to finally elucidate the three-dimensional structure of infectious prions.


Assuntos
Ressonância Magnética Nuclear Biomolecular/métodos , Proteínas Priônicas/metabolismo , Príons/metabolismo , Animais , Arvicolinae , Sistema Nervoso Central/patologia , Sulfato de Dextrana/farmacologia , Modelos Animais de Doenças , Camundongos Transgênicos , Doenças Priônicas/patologia , Estrutura Terciária de Proteína , Deficiências na Proteostase/patologia
13.
IEEE Int Conf Rehabil Robot ; 2019: 494-498, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31374678

RESUMO

INTRODUCTION: People with arthrogryposis multiplex congenita (AMC) often have muscle weakness in the biceps that makes elbow flexion difficult. An elbow-flexion assist orthosis was designed using the force of springs, combined with a sliding joint, to apply appropriate elbow torque to aid a user in lifting her hand to her mouth. The sliding joint allows an increasing elbow torque despite a decreasing spring force. METHODS: The device was prototyped for a user with AMC. An occupational therapist measured the user's flexion with and without the device. Benchtop torque measurements were also determined and compared with user trials. RESULTS: The assist orthosis applied an increasing torque as the elbow flexed, thereby allowing the subject to reach her mouth for feeding and then extend her elbow to a position of no applied torque. Without the device, the subject had active elbow flexion of 87 degrees. With the device, this flexion increased to 120 degrees. CONCLUSION: The novel prototype is a lightweight, spring-powered flexion orthosis which can be made relatively easily and is potentially concealed under clothing. It provides the appropriate torque to move the hand against gravity and increases elbow-flexion of the user.


Assuntos
Artrogripose/fisiopatologia , Braquetes , Articulação do Cotovelo/fisiopatologia , Cotovelo/fisiopatologia , Músculo Esquelético/fisiopatologia , Feminino , Humanos
14.
Semin Musculoskelet Radiol ; 23(3): 197-226, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31163499

RESUMO

The concept of hip impingement and hip-preserving surgery has been appreciated in more detail since 2001 when a new surgical approach was reported and a hypothesis linking femoroacetabular impingement (FAI) with osteoarthritis was presented. Paralleling the introduction of hip arthroscopy, these events led to an increasing interest in the hip, and the number of publications has risen rapidly over the past 15 years, despite limited evidence levels and inconsistent methodology. Accordingly, etiology, diagnosis, prognosis, and the effects of treatment for FAI are still elusive due to a number of uncertainties and a lack of clear diagnostic criteria.Future research must focus on developing high-quality scientific studies, so thorough and reproducible methodology is needed. This review provides researchers, radiologists, and clinicians with a comprehensive approach to hip imaging with a focus on strategies to help guide the clinical diagnosis. Using evidence from current literature and knowledge from experienced clinicians, some of the imaging methodology challenges are deciphered.


Assuntos
Diagnóstico por Imagem/métodos , Impacto Femoroacetabular/diagnóstico por imagem , Articulação do Quadril/diagnóstico por imagem , Osteoartrite do Quadril/diagnóstico por imagem , Artroscopia , Impacto Femoroacetabular/cirurgia , Articulação do Quadril/cirurgia , Humanos , Osteoartrite do Quadril/cirurgia
15.
J Neurosci Methods ; 324: 108310, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31175892

RESUMO

BACKGROUND: Developing methods to accelerate improvements in motor function are welcomed in clinical practice. Therefore, the aim of this study is to describe changes in brain activity related to the execution of motor tasks implemented on a software - the NeuroMaze - developed specifically to stimulate speed-accuracy tradeoff. NEW METHOD: The NeuroMaze was tested in eleven young and healthy individuals in a single experimental session. The tasks consisted in moving a square appearing on the monitor by holding and dragging it with a mouse across paths of different widths (wide [2 cm] vs intermediate [1.5 cm] vs narrow [1 cm] widths). The mouse cursor speed and scalp electroencephalography (EEG) from the frontal, somatosensory and motor areas were recorded. RESULTS: The mouse speed is reduced by 15 ±â€¯6% and 48 ±â€¯7% from the wide to the intermediate and narrow paths respectively (p < 0.005). Moreover, there was a greater beta EEG relative power in the narrow path in the frontal area of the brain when compared to the wide path (p < 0.05). Similarly, the narrow path reduced the gamma EEG relative power in motor/sensorimotor areas when compared to the wide path (p < 0.05). COMPARISON WITH EXISTING METHODS: The NeuroMaze is introduced as a method to elicit speed-accuracy tradeoff, and the authors are not aware of specific methods to establish fair comparisons. CONCLUSION: The NeuroMaze creates conditions to stimulate brain areas related to motor planning, sensory feedback and motor execution using speed-accuracy tradeoff contexts. Therefore, the NeuroMaze may induce adaptations in patients undergoing upper limb rehabilitation.

16.
J Biomech ; 90: 92-102, 2019 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-31113575

RESUMO

A novel metric for the validation of musculoskeletal models is proposed, the reachable 3-D workspace (RWS). This new metric was used to compare a generic model scaled in a standard manner to a more subject-specific model. An experimental protocol for assessing the RWS was performed by ten participants for four distinct hand-payload cases. In addition, isometric individual strength measurements were collected for 12 different directions. The strength of subject-specific musculoskeletal models was then computed using the following assumptions: (1) standard routines including the length-mass-fat (LMF) scaling law; (2) the isometric strengths of the muscle elements were optimized to the individual strength measurements using joint strength factors (JSF). The RWS of each participant was subsequently estimated from each of the scaling approaches, LMF and JSF, for the four load cases. The experimental RWS showed that the volume and shape decreased with increasing hand-payload for every participant. The lateral and frontal far-from-torso aspects of the RWS were reduced the most. These trends were reproduced by both strength scaling approaches, but the LMF-scaled models were not able to track the overall RWS volume decrease with increasing payload, since they proved to be weaker than the participants. On the other hand, the optimised JSF subject-specific models performed better on the prediction of the RWS for all payload cases across participants. The RWS can potentially be further used as a subject-specific musculoskeletal model validation, enabling quantification of the volume and shape differences between experimentally and model-predicted RWSs.

17.
Med Hypotheses ; 127: 49-56, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31088647

RESUMO

BACKGROUND: Valve-in-valve is established as a safe and efficient alternative to redo surgery in the treatment of structural valve deterioration (SVD). In vitro models rely on the radiopaque landmarks of undeteriorated tissue valves to establish the optimal implantation level of the transcatheter heart valves inside the deteriorated valves. In computed assisted procedures, the radiopaque landmarks of the deteriorated valves may be used to guide valve implantation through image fusion. The purpose of this study is to determine whether SVD alters the radiopaque landmarks of stented tissue valves. METHODS: Our approach was based on the computation of relevant anatomical measurements from CT images. Radiopaque landmarks of degenerated bioprostheses and the corresponding undeteriorated valves were extracted to create surface meshes and cloud points using grey-level thresholding. 3D registration using an iterative closest point algorithm was used to align the corresponding cloud points, while the modified Hausdorff Distance was applied to determine the differences between them. RESULTS: The proposed evaluation was performed on 19 degenerated tissue valves. 15 valves were scanned from patients evaluated for valve-in-valve procedures, and 4 bioprostheses were scanned after surgical extraction during redo aortic valve replacement. All the degenerated valves were compared to the corresponding undeteriorated models. Overall, the mean difference between degenerated and undeteriorated valves was 0.33 ±â€¯0.12 mm. The maximum observed registration error was 0.66 mm. CONCLUSIONS: Our study demonstrates no significant difference between the radiopaque landmarks of deteriorated and undeteriorated bioprostheses after the occurrence of SVD. Our findings suggest therefore that SVD does not alter radiopaque landmarks of stented tissue valves. These results validate in-vitro studies of optimal transcatheter heart valves implantation inside deteriorated tissue valves based on their radiopaque landmarks, and allow the use of non-deteriorated valves' imaging features in computer assisted valve-in-valve procedures.


Assuntos
Valva Aórtica/cirurgia , Bioprótese , Doenças das Valvas Cardíacas/cirurgia , Implante de Prótese de Valva Cardíaca , Próteses Valvulares Cardíacas , Algoritmos , Valva Aórtica/diagnóstico por imagem , Fluoroscopia , Humanos , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Desenho de Prótese , Reprodutibilidade dos Testes , Estudos Retrospectivos , Stents , Telas Cirúrgicas
18.
Arch Virol ; 164(5): 1479-1483, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30848387

RESUMO

Eight different double-stranded RNA (dsRNA) molecules were found in the wild-type fungal strain Botrytis cinerea CCg427. The electrophoretic profile displayed molecules with approximate sizes of 1, 1.3, 1.6, 1.8, 3.3, 4.1, 6.5, and 12 kbp. Sequences analysis of the molecules in the 6.5-kbp band revealed the presence of two different dsRNA molecules (dsRNA-1 and dsRNA-2) of 6192 and 5567 bp. Each molecule contained a unique ORF (5487 and 4836 nucleotides in dsRNA-1 and dsRNA-2, respectively). The ORF of dsRNA-1 encodes a 205-kDa polypeptide that shares 58% amino acid sequence identity with the RNA-dependent RNA polymerase (RdRp) encoded by dsRNA-1 of Alternaria sp. SCFS-3 botybirnavirus (ABRV1), whereas the ORF of dsRNA-2 encodes a 180-kDa polypeptide that shares 52% amino acid sequence identity with an unclassified protein encoded by dsRNA-2 of ABRV1. Genome organization and phylogenetic analysis based on the amino acid sequences of RdRps in members of different dsRNA virus families showed that the dsRNAs in the 6.5-kbp band correspond to the genome of a new botybirnavirus that we have named "Botrytis cinerea botybirnavirus 1".


Assuntos
Botrytis/virologia , Micovírus/genética , Genoma Viral/genética , Vírus de RNA/genética , RNA Viral/genética , Sequência de Aminoácidos , Micovírus/classificação , Micovírus/isolamento & purificação , Filogenia , RNA Replicase/genética , Vírus de RNA/classificação , Vírus de RNA/isolamento & purificação , Proteínas Virais/genética
19.
Hortic Res ; 6: 6, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30603092

RESUMO

The timing of fruit maturity is an important trait in sweet cherry production and breeding. Phenotypic variation for phenology of fruit maturity in sweet cherry appears to be under strong genetic control, but that control might be complicated by phenotypic instability across environments. Although such genotype-by-environment interaction (G × E) is a common phenomenon in crop plants, knowledge about it is lacking for fruit maturity timing and other sweet cherry traits. In this study, 1673 genome-wide SNP markers were used to estimate genomic relationships among 597 weakly pedigree-connected individuals evaluated over two seasons at three locations in Europe and one location in the USA, thus sampling eight 'environments'. The combined dataset enabled a single meta-analysis to investigate the environmental stability of genomic predictions. Linkage disequilibrium among marker loci declined rapidly with physical distance, and ordination of the relationship matrix suggested no strong structure among germplasm. The most parsimonious G × E model allowed heterogeneous genetic variance and pairwise covariances among environments. Narrow-sense genomic heritability was very high (0.60-0.83), as was accuracy of predicted breeding values (>0.62). Average correlation of additive effects among environments was high (0.96) and breeding values were highly correlated across locations. Results indicated that genomic models can be used in cherry to accurately predict date of fruit maturity for untested individuals in new environments. Limited G × E for this trait indicated that phenotypes of individuals will be stable across similar environments. Equivalent analyses for other sweet cherry traits, for which multiple years of data are commonly available among breeders and cultivar testers, would be informative for predicting performance of elite selections and cultivars in new environments.

20.
Environ Sci Pollut Res Int ; 26(27): 27579-27589, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29594880

RESUMO

Hydrothermal carbonization (HTC) is a thermochemical process carried out in an aqueous medium. It is capable of converting biomass into a solid, carbon-rich material (hydrochar), and producing a liquid phase (process water) which contains the unreactive feedstock and/or chemical intermediates from the carbonization reaction. The aim of this study was to evaluate the characteristics of process water generated by HTC from vinasse and sugarcane bagasse produced by sugarcane industry and to evaluate its toxicity to both marine (using Artemia salina as a model organism) and the terrestrial environment (through seed germination studies of maize, lettuce, and tomato). The experiments showed that concentrated process water completely inhibited germination of maize, lettuce, and tomato seeds. On the other hand, diluted process water was able to stimulate seedlings of maize and tomato and enhance root and shoot growth. For Artemia, the LC50 indicated that the process water is practically non-toxic; however, morphological changes, especially damages to the digestive tube and antennas of Artemia, were observed for the concentration of 1000 mg C L-1.


Assuntos
Carbono/química , Saccharum/química , Biomassa , Germinação , Indústrias , Alface/química , Lycopersicon esculentum/química , Sementes/química , Água , Zea mays/química
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