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1.
Aust Endod J ; 2021 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-33682987

RESUMO

The aim of the study was to evaluate the cytotoxicity of different glycolic acid concentrations (GA) and its effects on dentinal microhardness. Cytotoxicity was evaluated after inoculation of test irrigants in the lymphocyte primary culture for 3 min. The tested substances were distilled water(DW); 17% EDTA; QMix; 10% GA; 17% GA; and 25% GA. Counting of total, live and dead cells was performed, obtaining the average percentage of dead cells of each group. For microhardness evaluation, 60 root dentin samples were divided into the same tested groups (n = 10) and immersed in test irrigants for 3 min. Dentin microhardness was evaluated by Vicker test. Specific statistical analysis was made in both tests. Results showed significant lower cytotoxicity for QMix and 10% GA (P < 0.05). Moreover, all test irrigants presented similar values of microhardness than the control group (P > 0.05). In conclusion, lower GA concentration can be an alternative for final irrigation on endodontics.

2.
Photodiagnosis Photodyn Ther ; 33: 102151, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33346059

RESUMO

INTRODUCTION: This study aimed to evaluate the influence of glycolic acid-based final irrigant for photosensitizer removal of photodynamic therapy on the microhardness and colour change of the dentin structure. METHODS: Eighty extracted single-rooted human incisors were used. Sample preparation and root split resulted in 160 samples, 80 samples being used for microhardness and 80 samples for colour change evaluation. In the first, PDT protocol was performed and 80 samples were randomly divided into 4 groups (n = 20), according to the final irrigation protocol: distilled water (DW); 17 % ethylenediaminetetraacetic acid (EDTA); QMix; 17 % glycolic acid (GA). Microhardness was evaluated using the Vicker tester, before and after, PDT and final irrigation protocols, calculating the percentage of microhardness reduction. In the second evaluation, PDT and final irrigation protocols were performed in the same way. Colour change was evaluated using digital spectrophotometer before and after these protocols, calculating the ΔE colour change using the CIELAB system (L*a*b* values). Specific statistical analysis was performed for both evaluations (α = 5%). RESULTS: The highest percentage of microhardness reduction was observed in 17 % EDTA, QMix and 17 % GA groups, with no significant difference among them (p > 0.05). Furthermore, none of these protocols was effective in photosensitizer removal, and all final irrigation protocols were statically similar to control group (p > 0.05). CONCLUSIONS: GA promotes microhardness reduction and also contributes to the colourization of dentin structure during the photosensitizer removal process, followingPDT .

3.
Biosci. j. (Online) ; 36(6): 2287-2296, 01-11-2020. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1148387

RESUMO

The aim of this study was to evaluate the effectiveness of grape seed extract (GSE)-based intracanal dressings against Enterococcus faecalis (E. faecalis) and its influence on dentin microhardness and bond strength of the filling material. The root canals of 126 human teeth were distributed into three test groups: antimicrobial activity (60 teeth), dentin microhardness (30 teeth) and bond strength (36 teeth). In all three groups, specimens were subdivided into six groups, according to intracanal dressing protocols: G1 ­ distilled water (DW); G2 ­ 2% chlorhexidine gel (CHX); G3 ­ calcium hydroxide (Ca[OH]2)+DW; G4 ­ GSE+DW; G5 ­ Ca(OH)2+CHX; G6 ­ GSE+CHX. The counting of colony-forming units (CFUs), the Vickers microhardness tester and the push-out test were performed to evaluate the antimicrobial activity, dentin microhardness and bond strength, respectively. Specific statistical analysis was performed for each evaluation (α=5%). The greatest bacterial reduction was observed in G5 (Ca[OH]2+CHX) and G6 (GSE+CHX) (p<0.05). There was no statistically significant difference among groups in the dentin microhardness evaluation (p<0.05). The highest bond strength in the immediate evaluation was observed in G4 (GSE+DW) and G6 (GSE+CHX), whereas the highest bond strength after 12 months of storage was observed in G2 (CHX), G3 (Ca[OH]2+DW), G4 (GSE+DW), and G6 (GSE+CHX) (p<0.05). After the storage period, bond strength was increased in G2 (CHX) and G3 (Ca[OH]2+DW), and remained unchanged in G4 (GSE+DW) and G6 (GSE+CHX) (p<0.05). GSE-based intracanal dressings have antimicrobial potential against E. faecalis, have no influence in dentin microhardness and preserve the high bond strength of filling materials for root dentin over time.


O objetivo deste estudo foi avaliar a eficácia de medicamentos intracanal à base de extrato de semente de uva (GSE) contra Enterococcus faecalis (E. faecalis) e sua influência na microdureza da dentina e na resistência de união do material de obturação. Os canais radiculares de 126 dentes humanos foram distribuídos em três grupos de teste: atividade antimicrobiana (60 dentes), microdureza da dentina (30 dentes) e resistência adesiva (36 dentes). Nos três grupos, as amostras foram subdivididas em seis grupos, de acordo com os protocolos de curativos intracanal: G1 ­ água destilada (DW); G2 ­ gel de clorexidina a 2% (CHX); G3 ­ hidróxido de cálcio (Ca[OH]2) +DW; G4 ­ GSE+DW; G5 ­ Ca(OH)2+CHX; G6 ­ GSE+CHX. A contagem de unidades formadoras de colônias (UFCs), o testador de microdureza Vickers e o teste push-out foram realizados para avaliar a atividade antimicrobiana, a microdureza da dentina e a resistência adesiva, respectivamente. Análise estatística específica foi realizada para cada avaliação (α=5%). A maior redução bacteriana foi observada no G5 (Ca[OH]2+CHX) e G6 (GSE+CHX) (p<0,05). Não houve diferença estatisticamente significativa entre os grupos na avaliação da microdureza da dentina (p<0,05). A maior resistência adesiva na avaliação imediata foi observada no G4 (GSE+DW) e G6 (GSE+CHX), enquanto a maior resistência adesiva após 12 meses de armazenamento foi observada no G2 (CHX), G3 (Ca[OH]2+DW), G4 (GSE+DW) e G6 (GSE+CHX) (p<0,05). Após o período de armazenamento, a resistência de união aumentou no G2 (CHX) e G3 (Ca[OH]2+DW), permanecendo inalterada no G4 (GSE+DW) e G6 (GSE+CHX) (p<0,05). Os medicamentos intracanal à base de GSE têm potencial antimicrobiano contra E. faecalis, não influenciam na microdureza da dentina e preservam a alta resistência adesiva dos materiais de obturação da dentina radicular ao longo do tempo.


Assuntos
Dentina , Extrato de Sementes de Uva , Anti-Infecciosos
4.
Biosci. j. (Online) ; 36(5): 1785-1793, 01-09-2020.
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1147935

RESUMO

This study objectives to evaluate the fracture strength of upper central incisors (UCI) restored with composite resin (CR) in Class III cavities and endodontically treated teeth with or without glass fiber post (GFP), analyzing their failure mode. Sixty human UCI were randomly divided into four experimental groups: endodontically treated teeth without GFP (G1), endodontically treated teeth with GFP (G2), teeth with mesial/distal Class III cavities restored with CR without GFP (G3), and teeth with mesial/distal Class III cavities restored with CR with GFP (G4). The samples were submitted to the fracture strength test in a universal testing machine with a compression shear load applied at speed of 1.0 mm/min until fracture occurred. The data were submitted to one-way ANOVA (α=0.05) and the samples were analyzed for failure mode. The analysis did not show a significant statistical difference in fracture strength between the groups (p>0.05). The results showed that only endodontically treated teeth (G1) (753.4N) presented behavior similar to teeth with GFP (G2) (702.1N). The same occurred when comparing teeth with Class III cavities without GFP (G3) (670.2 N) and with GFP (G4) (746.1N). It can be concluded that glass fiber posts do not change the fracture strength of incisors with endodontic treatment and Class III cavities. KEYWORDS: Cavities. Composite resins. Endodontically treated teeth. Fracture. INTRODUCTION Endodontically treated teeth with little remaining crown structure are considered more fragile than healthy teeth due to the loss of healthy dental structure (KARZOUN et al., 2015). In such cases, the use of an intraradicular post is indicated to promote retention to the future restoration. For many years, the cast metal cores (CMC) and the intraradicular metal posts were the only form of tooth retention (SMITH et al., 1998). However, research indicates that such posts may cause irreparable fractures (BARCELLOS et al., 2013; GUO et al., 2016), for requiring the wear of the dentin structure (CHUANG et al., 2010) and for presenting both a high modulus of elasticity (SANTOS-FILHO et al., 2008; FARINA et al., 2015) and the possibility of corrosion (LASSILA et al., 2004)thus limiting their use. Glass fiber posts (GFP) have advantages over metal posts because they provide better aestheticsand dentin-like biomechanical properties (GORACCI et al., 2007; MARTELLI et al., 2008; SANTOS-FILHO et al., 2008; MOSHARRAF et al., 2012; AMARNATH et al., 2015). Their retention is related to specific characteristics such as post length, type of cement used, and amount of remaining tooth structure (ALOMARI et al., 2011; MAKADE et al., 2011; INAGAKI et al., 2014; KHOROUSHI et al., 2016). It is also known that the clinical applicability of GFP is directly related to the reconstruction of the dental element lost (MAKADE et al., 2011; AMBICA et al., 2013; WANDSCHER et al., 2014; REZAEI et al., 2015; RAHMAN et al., 2016). Thus, an intraradicular post should be selected with caution, verifying the amount of remaining tooth structure, root canal anatomy, post length and diameter, as well as the physical properties and modulus of elasticity (ALOMARI et al., 2011; HATTA et al., 2011; LE BELL-RÖNNLÖF et al., 2011). The higher the amount of remaining crown, the higher the resistance of the dental element (ZOGHEIB et al., 2008; Received: 02/06/19 Accepted: 30/12/19


Este estudo objetiva avaliar a resistência à fratura de incisivos centrais superiores (ICS) restaurados com resina composta (RC) em cavidades Classe III e dentes tratados endodonticamente com ou sem pino de fibra de vidro (PFV), analisando seu padrão de fratura. Sessenta ICS humanos foram divididos aleatoriamente em quatro grupos experimentais: dentes tratados endodonticamente sem PFV (G1), dentes tratados endodonticamente com PFV (G2), dentes com cavidades mesiais/distais Classe III restauradas com RC sem PFV (G3), e dentes com cavidades mesiais/distais Classe III restauradas com RC com PFV (G4). As amostras foram submetidas ao teste de resistência à fratura em uma máquina universal de ensaios com uma carga de cisalhamento de compressão aplicada na velocidade de 1,0 mm / min até a ocorrência da fratura. Os dados foram submetidos à ANOVA unidirecional (α=0,05) e as amostras foram analisadas quanto ao modo de falha. A análise não mostrou diferença estatisticamente significativa na resistência à fratura entre os grupos (p>0,05). Os resultados mostraram que os dentes apenas tratados endodonticamente (G1) (753,4N) apresentaram comportamento semelhante aos dentes com PFV (G2) (702,1N). O mesmo ocorreu ao comparar dentes com cavidades Classe III sem PFV (G3) (670.2 N) e com PFV (G4) (746.1N). Pode-se concluir que pinos de fibra de vidro não alteram a resistência à fratura de incisivos com tratamento endodôntico e cavidades Classe III.

5.
Dent Mater ; 36(10): e302-e308, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32811665

RESUMO

OBJECTIVES: To investigate the role of proteoglycans (PGs) on the physical properties of the dentin matrix and the bond strength of methacrylate resins with varying hydrophilicities. METHODS: Dentin were obtained from crowns of human molars. Enzymatic removal of PGs followed a standard protocol using 1 mg/mL trypsin (Try) for 24 h. Controls were incubated in ammonium bicarbonate buffer. Removal of PGs was assessed by visualization of glycosaminoglycan chains (GAGs) in dentin under transmission electron microscopy (TEM). The dentin matrix swelling ratio was estimated using fully demineralized dentin. Dentin wettability was assessed on wet, dry and re-wetted dentin surfaces through water contact angle measurements. Microtensile bond strength test (TBS) was performed with experimental adhesives containing 6% HEMA (H6) and 18% HEMA (H18) and a commercial dental adhesive. Data were statistically analyzed using ANOVA and post-hoc tests (α = 0.05). RESULTS: The enzymatic removal of PGs was confirmed by the absence and fragmentation of GAGs. There was statistically significant difference between the swelling ratio of Try-treated and control dentin (p < 0.001). Significantly lower contact angle was found for Try-treated on wet and dry dentin (p < 0.002). The contact angle on re-wet dentin was not recovered in Try-treated group (p = 0.9). Removal of PGs significantly improved the TBS of H6 (109% higher, p < 0.001) and H18 (29% higher, p = 0.002) when compared to control. The TBS of commercial adhesive was not affected by trypsin treatment (p = 0.9). SIGNIFICANCE: Changing the surface energy of dentin by PGs removal improved resin adhesion, likely due to more efficient water displacement, aiding to improved resin infiltration and polymerization.

6.
Sci Rep ; 10(1): 7313, 2020 04 30.
Artigo em Inglês | MEDLINE | ID: mdl-32355304

RESUMO

The objective of this study was to evaluate the effects of glycolic acid (GA) (with pH 1.2 and 5) and ethylenediaminetetraacetic acid (EDTA) on the chemical and mechanical properties of dentin to investigate the potential use of GA as final irrigant in the root canal therapy. Specifically, changes in microhardness, smear layer removal, erosion, mineral content distribution, apatite/collagen ratio and flexural strength of mineralized dentin treated with GA were assessed. Saline solution was used as a negative control. Knoop microhardness (KHN) was measured on the root canal lumen of root segments. Dentin beams were used for 3-point flexural strength (σ) test. Scanning electron microscopy (SEM) images of root sections were obtained for evaluation of smear layer removal and dentin erosion on root segments and energy dispersive X-ray spectroscopy (EDS) was used for mineral content distribution. The apatite/collagen ratio (A/C) in dentin powder were examined by Fourier transform infrared (FTIR) spectroscopy. KHN, σ and A/C results were statistically analyzed with ANOVA and Tukey tests (α = 0.05). Smear layer and dentin erosion scores were analyzed with Kruskal-Wallis and Dunn tests (α = 0.05). Root dentin treated with EDTA and GA presented similar KHN regardless of the pH (p > 0.05). However, KHN was significantly reduced in EDTA and GA groups when compared to control group (p<0.001). GA showed the same ability to remove the smear layer and to cause dentin erosion as EDTA. EDS results showed that the GA and EDTA solutions did not alter the dentin mineral content distribution. The apatite/collagen ratio reduced with all irrigant solution and was the lowest with GA pH 5 (p<0.001), while σ was not significantly affected by the experimental solutions (p = 0.559). It can be concluded that GA has similar ability to remove the smear layer than EDTA. GA does not affect negatively the chemical/mechanical properties and it does not increase dentin erosion. The use of GA with low pH seems to promote less change in collagen/apatite ratio, but further studies are needed to establish an ideal clinical protocol. Therefore, this study supports the potential use of GA as an alternative final irrigation solution for root canal preparation.

7.
Eur Endod J ; 5(1): 35-39, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32342036

RESUMO

Objective: The purpose of this research was to compare relative effectiveness of sodium hypochlorite 5.25% (NaOCl), 2% chlorhexidine gel (CHX) and 6.5 % grape seed extract (GSE) against Enterococcus faecalis using instrument Reciproc R25 in root canal preparation. Methods: Forty-five mesiobuccal root canals from extracted human maxillary molars were collected and infected with Enterococcus faecalis. The samples were divided into five groups according to the different types of irrigants: saline (positive control) (n=5); in the other groups were used 10 root canals for each group: NaOCl+EDTA; CHX gel+EDTA; GSE solution+EDTA; GSE gel+EDTA. All the groups were prepared with reciprocating instruments Reciproc R25. Bacterial reduction was measured by two-way ANOVA (P<0.001) followed by Tukey HSD post-hoc tests, from the counting of colony forming units (CFUs) from samples collected before instrumentation and after. The significance level established at 5% (P<0.05). Results: The group prepared with the NaOCl resulted in highest antimicrobial capacity among of all (P>0.05), followed by CHX and GSE gel (P<0.05). Control and GSE solution showed similar results (P<0.05) and resulted in the lowest percentage of the reduction of the microorganism into the root canals. Conclusion: NaOCl had the higher elimination capacity of Enterococcus faecalis than GSE and CHX.

8.
Aust Endod J ; 46(2): 191-196, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31814249

RESUMO

This study evaluated the antimicrobial effectiveness of 6.5% Vitis vinifera grape seed extract (GSE) against Enterococcus faecalis biofilm using confocal laser scanning microscopy (CLSM). Saline solution (SS), 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) were used for comparison. Dentin discs were inoculated with E. faecalis strain establishing a 3-week-old biofilm. Discs (n = 10) were exposed to 5.25% NaOCl, 2% CHX, 6.5% GSE and SS (negative control) for 10 min. Discs were stained with the fluorescent LIVE/DEAD-BacLight™ dye and analysed using CLSM. The proportion of dead cells in biofilm was analysed using one-way anova and Tukey tests (P < 0.05). A higher proportion of dead cells was found in GSE group compared with CHX and SS (P < 0.05). NaOCl group was associated with the highest proportion of dead cells (P < 0.05). GSE presented antimicrobial activity against E. faecalis; however, NaOCl was the most effective irrigant solution. GSE was more effective than CHX and SS.


Assuntos
Enterococcus faecalis , Extrato de Sementes de Uva , Biofilmes , Clorexidina , Dentina , Microscopia Confocal , Irrigantes do Canal Radicular , Hipoclorito de Sódio
9.
Gen Dent ; 68(1): 41-44, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31859661

RESUMO

The objective of this study was to evaluate the antimicrobial capacity of glycolic acid (GA) at different concentrations as a final irrigant during the preparation of root canals. The sample consisted of 77 extracted single-rooted human teeth with complete root formation, no previous endodontic treatment, and a root length of at least 14 mm. The root canals were prepared in a standardized manner with a rotary file system. During this process, irrigation was performed with 2.5% sodium hypochlorite (NaOCl), and the final irrigant was 17% ethylenediaminetetraacetic acid (EDTA). After the root canal sterilization procedure, Enterococcus faecalis was cultured in a Petri dish, and 70 sterilized root canals were inoculated with a suspension containing 3.0 × 108 colony-forming units (CFUs) per milliliter. The roots were divided into 7 groups (n = 10) according to the following solutions: 0.9% sodium chloride (NaCl); 6% NaOCl; 17% EDTA; 10%, 17%, or 25% GA; or 17% citric acid (CA). The capacity of the different substances to reduce E faecalis was evaluated by counting the CFUs before and after treatment with the final irrigant solutions. Data were subjected to an analysis of variance and the Tukey test at a 5% significance level. The greatest bacterial reduction was observed in the group irrigated with NaOCl (P < 0.05). There were no statistically significant differences among the groups irrigated with GA in different concentrations (P > 0.05), but they all demonstrated greater disinfection capacity than CA and EDTA (P < 0.05). CA showed significantly greater antimicrobial capacity than EDTA (P < 0.05). EDTA showed significantly greater antimicrobial capacity only in relation to NaCl (P < 0.05). At different concentrations, GA demonstrated greater capacity to eliminate E faecalis from root canals than did EDTA.


Assuntos
Anti-Infecciosos , Glicolatos , Irrigantes do Canal Radicular , Preparo de Canal Radicular , Anti-Infecciosos/farmacologia , Cavidade Pulpar , Ácido Edético , Enterococcus faecalis , Glicolatos/farmacologia , Humanos , Preparo de Canal Radicular/métodos , Hipoclorito de Sódio
10.
Photodiagnosis Photodyn Ther ; 29: 101625, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31866534

RESUMO

INTRODUCTION: This study aimed to evaluate the antimicrobial action of calcium hypochlorite(Ca[OCl]2) and sodium hypochlorite (NaOCl) with reciprocating instrumentation and photodynamic therapy(PDT), and its influence on root dentin structure. METHODS: One hundred and ten human teeth were used to antimicrobial evaluation, inoculated with Enterococcus faecalis and divided into 11 groups (n = 10): G1 - distilled water(control); G2-1 % NaOCl; G3-5.25 % NaOCl; G4-1 % Ca(OCl)2; G5-5.25 % Ca(OCl)2; G6-PDT; G7-distilled water + PDT; G8-1 % NaOCl + PDT; G9-5.25 % NaOCl + PDT; G10-1 % Ca(OCl)2+PDT; G11-5.25 % Ca(OCl)2+PDT. In all groups, except G6, the root canals were instrumented with #R50 Reciproc file in the working length in association with tested chemical auxiliary substance. The counting of colony forming units (CFUs) was performed to calculate the bacterial percentage reduction of each group. Following, 55 bovine teeth were used to microhardness evaluation and divided into the same 11 groups (n = 5), with no instrumentation and immersion in the tested protocols. The modification of root dentin microhardness was evaluated by using the Vicker tester. Finally, 33 bovine teeth were used to organic components evaluation and divided into the same 11 groups (n = 3), with no instrumentation and immersion in the tested protocols. The modification of organic components of root dentin was evaluated by light microscopy. Specific statistical analysis was performed for each evaluation. RESULTS: The higher bacterial reduction was observed in groups 3,4,5,8,9,10 and 11, with no statistical difference between them (p > 0.05). The higher microhardness reduction was observed in groups 8,9,10 e 11, with no statistical difference between them (p > 0.05). The highest modification on organic components was observed in groups 3,5,9 and 11, with no statistical difference between them (p > 0.05). CONCLUSIONS: The association of Ca(OCl)2, reciprocating instrumentation and PDT promotes effective antimicrobial action. Moreover, lower modification was induced in microhardness and organic components of root dentin, by using Ca(OCl)2 in low concentration associated to PDT.

11.
Mater Sci Eng C Mater Biol Appl ; 106: 110283, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31753393

RESUMO

The aim of this study was to characterize glycolic acid (GA) and examine its effects on powder and flexural strength of dentin. Particle size and energy-dispersive EDS in GA powder was performed for chemical analysis. Surface tension and pH levels of ethylenediaminetetraacetic acid (EDTA), citric acid (CA), and GA solutions were evaluated at different times and temperatures. Dentin powder and mineralized dentin beams were immersed for 1 min in EDTA, CA, or GA solutions and subjected to Fourier transform infrared spectroscopy for apatite/collagen ratio analysis and 3-point flexure test, respectively. GA showed the largest particle size (µm), and its surface tension was similar to that of EDTA and CA. Surface tension decreased in solutions of higher concentrations. GA showed pH stability at all times and temperatures evaluated. The apatite/collagen ratio reduced with increased GA concentrations, while flexural strength was not significantly affected by GA concentration. GA seems a good choice as a final irrigation solution after root canal preparation.


Assuntos
Dentina/química , Glicolatos/química , Ácido Cítrico/química , Colágeno/química , Colágeno/metabolismo , Cavidade Pulpar/efeitos dos fármacos , Dentina/efeitos dos fármacos , Ácido Edético/química , Resistência à Flexão , Glicolatos/farmacologia , Humanos , Concentração de Íons de Hidrogênio , Tamanho da Partícula , Desnaturação Proteica , Irrigantes do Canal Radicular/química , Irrigantes do Canal Radicular/farmacologia , Tensão Superficial , Temperatura
12.
J Contemp Dent Pract ; 20(6): 657-663, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31358705

RESUMO

AIM: The aim of this study is to assess three protocols for removing a eugenol-based filling endodontic sealer from root dentin and the bond strength of fiberglass posts luted with resin cement. MATERIALS AND METHODS: Sixty single-rooted bovine teeth were prepared and filled with the Endofill endodontic sealer and gutta-percha cones. After 7 days, 9 mm of the filling was removed and divided into five groups (n = 12) according to the cleaning protocol: G1 (negative control), unfilled; G2 (positive control), saline solution; G3, 95% alcohol; G4, amyl acetate; and G5, Largo bur. The canals were hybridized and fiberglass posts were luted with resin cement. They were cross-sectioned in slices of 1 mm and subjected to the push-out test. The other samples were sectioned longitudinally and visualized in a scanning electron microscope (SEM), and the existing chemical elements were quantified by energy dispersive spectroscopy (EDS). The statistical analysis used variance analysis (ANOVA)-one way and Fisher's test, at a 5% significance level. RESULTS: The 95% alcohol was more effective for cleaning the canal, resulting in a clean dentinal surface and bond strength statistically similar to the negative control. The Largo bur was also statistically similar to the negative control, with only a few debris impregnated on the wall. Amyl acetate showed more sealer residues on the canal walls, with a consequent lower bond strength value than the other groups, only higher than the positive control. CONCLUSION: The 95% alcohol and Largo burs may be used after removing the canal filling, so that the bond strength is improved when using the eugenol-based sealer.


Assuntos
Colagem Dentária , Técnica para Retentor Intrarradicular , Materiais Restauradores do Canal Radicular , Animais , Bovinos , Dentina , Resinas Epóxi , Eugenol , Guta-Percha
13.
Photodiagnosis Photodyn Ther ; 26: 224-228, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30965149

RESUMO

INTRODUCTION: This study aimed to evaluate the influence of final irrigation protocols and type of resin cement on the bond strength of glass fiber posts (GFPs) in root dentin previously treated with photodynamic therapy (PDT). METHODS: One hundred root canals were prepared up to #4 Gates Glidden drill to receive the GFPs. All samples were subjected to PDT, and randomly divided into five groups (n = 20) according to final irrigation protocol: distilled water + ultrasonic activation [US]; 17% EDTA; QMix; 17% EDTA + US; and QMix + US. Each group was randomly divided into 2 subgroups (n = 10) according to the type of resin cement used for cementation of GFPs: Rely-X ARC dual-resin; or Rely-X U200 (#M, St Paul, MN, USA) self-adhesive resin cement. Bond strength was evaluated using the push-out test. Failure patterns were observed under an optical microscope. Bond strengths were compared using two-way ANOVA, followed by the Tukey post-hoc test; failure modes were compared using the chi-squared test (α = 5%). RESULTS: The use of US over final irrigants improved the bond strength of GFPs, regardless of resin cement used (p < 0.05). The type of resin cement did not influence the bond strength of GFPs (p < 0.05). There was a higher predominance of cohesive failure in all groups, regardless of resin cement tested (p < 0.05). CONCLUSIONS: The use of US on final irrigation improved the bond strength of GFPs, while the type of resin cement (dual or self-adhesive) did not influence the bond strength of GFPs in root dentin previously treated with PDT.


Assuntos
Dentina/efeitos dos fármacos , Fotoquimioterapia/métodos , Técnica para Retentor Intrarradicular , Cimentos de Resina/química , Irrigantes do Canal Radicular/farmacologia , Biguanidas/farmacologia , Ácido Edético/farmacologia , Humanos , Técnicas In Vitro , Teste de Materiais , Polímeros/farmacologia , Distribuição Aleatória , Ultrassom
14.
Mater Sci Eng C Mater Biol Appl ; 100: 323-329, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30948068

RESUMO

The aim of this study was to investigate the effects of glycolic acid (GA) on the microhardness, roughness, dentin mineral content distribution; smear layer removal and cytotoxicity. One hundred human teeth were randomly divided into six groups: distilled water (control group), 17% EDTA, 10% citric acid (CA), 5% GA, 10% GA, and, 17% GA. Microhardness and roughness were measured in the canal lumen. Scanning electron microscopy (SEM) images (2000×) for smear layer removal evaluation; energy dispersive X-ray spectroscopy (EDS) for chemical analysis. Cell viability assay was made on fibroblast cells. The lowest microhardness and higher roughness were observed for 17% GA. GA showed the ability to remove the smear layer to a similar level as EDTA and CA, with no statistical difference between the concentrations used. GA and CA were cytotoxic in a dose-dependent manner. GA showed potential as an endodontic agent for final irrigation in root canal terapies.


Assuntos
Endodontia , Glicolatos/farmacologia , Fenômenos Mecânicos , Irrigantes do Canal Radicular/farmacologia , Células 3T3 , Animais , Morte Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Dureza , Humanos , Camundongos , Camada de Esfregaço/patologia
15.
Odontology ; 107(3): 316-323, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30710179

RESUMO

Proteoglycans are biomacromolecules with significant biomineralization and structural roles in the dentin extracellular matrix. This study comprehensively assessed the mechanical properties and morphology of the dentin extracellular matrix following chemical removal of proteoglycans to elucidate the structural roles of proteoglycans in dentin. Dentin extracellular matrix was prepared from extracted teeth after complete tissue demineralization. Chemical removal of proteoglycans was carried-out using guanidine hydrochloride for up to 10 days. The removal of proteoglycans was determined by dimethylmethylene blue colorimetric assay and histological staining analyses using transmission electron microscopy and optical microscopy. The modulus of elasticity of dentin matrix was determined by a 3-point bending test method. Partial removal of proteoglycans induced significant modifications to the dentin matrix, particularly to type I collagen. Removal of proteoglycans significantly decreased the modulus of elasticity of dentin extracellular matrix (p < 0.0001). In conclusion, the subtle disruption of proteoglycans induces pronounced changes to the collagen network packing and the bulk modulus of elasticity of dentin matrix.


Assuntos
Dentina , Proteoglicanas , Colágeno Tipo I , Matriz Extracelular , Microscopia Eletrônica de Transmissão
16.
Biomed Res Int ; 2019: 2196519, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30719440

RESUMO

Objectives: The aim of this study was to evaluate the influence of ferule and the post type on the fracture strength and stress distribution in premolars. Materials and Methods: Forty human mandibular premolars were decoronated, allocated in four groups, and restored as follows: CPC-NF: cast post-and-core and absence of ferule; CPC-F: cast post-and-core and presence of ferule; FPC-NF: glass-fiber posts and absence of ferule; FPC-F: glass-fiber posts and presence of ferule. The fracture strength (FS) and failure patterns were evaluated. Finite element analysis (FEA) evaluated the stress distribution. Results: FS did not differ between CPCs and FPC either in presence or in absence of ferule. The presence of ferule increased FS with both post types. Mean values of FS for ferule groups were higher than functional or parafunctional loads reported in literature, which was not the case for FPC-NF when compared to parafunctional loads. FEA with a functional load showed slightly higher compressive stresses in dentin in the group CPC-NF, which was much lower than the compressive strength of dentin. Lower percentage of catastrophic failures was observed in nonferule groups irrespective of post type, which was explained by the stress concentration in the cervical root region when FEA with the FS load was simulated. Conclusion: Ferule effect was shown to be more important than post type in the analysis. Both posts showed potential to withstand functional loads irrespective of presence of ferule. However, the mean FS was lower than parafunctional loads for FPC in the absence of ferule.


Assuntos
Força Compressiva/fisiologia , Resistência à Flexão/fisiologia , Vidro/química , Fraturas dos Dentes/fisiopatologia , Dente Pré-Molar/fisiopatologia , Resinas Compostas/química , Materiais Dentários/química , Falha de Restauração Dentária , Análise do Estresse Dentário/métodos , Análise de Elementos Finitos , Humanos , Teste de Materiais/métodos , Cimentos de Resina/química , Estresse Mecânico
17.
Aust Endod J ; 45(1): 92-97, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30113742

RESUMO

The aim of this study was to evaluate the antimicrobial activity of sodium and calcium hypochlorite utilising reciprocating instrumentation. Sixty root canals were inoculated with E. faecalis for 14 days. Samples were divided into six groups according to decontamination protocol: G1: no treatment, G2: distilled water, G3: 2.5% sodium hypochlorite, G4: 2.5% calcium hypochlorite, G5: 5.25% sodium hypochlorite and G6: 5.25% calcium hypochlorite. Instrumentation was performed with Wave One reciprocating system (Dentsply Sirona Endodontics, York, PA, USA) in groups G2 to G6. Colony-forming units (CFUs) counting was performed and the data were subjected to Anova and Tukey (α  =  0.05). Group 1 and 2 showed the highest mean contamination, with a significant difference between them (P < 0.05). Groups 3, 4, 5 and 6 showed the lowest contamination means with no significant difference between them (P < 0.05). Sodium and calcium hypochlorite, in association with reciprocating instrumentation, can be an effective decontamination protocol in root canals infected with E. faecalis.


Assuntos
Enterococcus faecalis , Hipoclorito de Sódio , Cálcio , Descontaminação , Cavidade Pulpar , Irrigantes do Canal Radicular
18.
Aust Endod J ; 45(2): 177-183, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30105768

RESUMO

The aim of this study was to evaluate the bond strength between the fibre post and root dentin after different cleaning protocols to remove eugenol from dentin. The residues and elemental composition of dentin were analysed using SEM and EDS. Bovine incisor roots were obturated with eugenol-based sealer. The post space was prepared, and the roots were divided into the following groups according to the cleaning protocol: unfilled (negative control), saline solution (positive control), 70% ethanol, acetone and 70% isopropyl alcohol. The fibre posts were cemented and push-out test realised. Chemical analysis of the dentin was performed using SEM and EDS. The bond strength values were analysed by anova/Fisher's and were as follows: negative control (7.30 ± 2.77) = 70% ethanol (6.64 ± 2.91) = 70% isopropyl alcohol (5.01 ± 3.15) = acetone (4.42 ± 1.53) >positive control (1.29 ± 0.56). It was concluded that 70% ethanol may be a good cleaning protocol to remove eugenol from dentin.


Assuntos
Colagem Dentária , Técnica para Retentor Intrarradicular , Animais , Bovinos , Dentina , Adesivos Dentinários , Eugenol , Cimentos de Resina
19.
Periodontia ; 29(1): 30-36, 2019.
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-994608

RESUMO

O objetivo dessa revisão de literatura é verificar as estratégias de tratamento das lesões endo-periodontais. Foi realizada uma busca na literatura, nas seguintes bases de dados: Medline, Crochane, PubMed e Scielo, contendo artigos científicos dos últimos dez anos nos idiomas inglês e português usando os termos de pesquisa: "Tratamento" E/OU "Lesões Endo-periodontal" E/OU "doença periodontal" E/OU "Endodontia". As lesões endo-peridontais são caracterizadas pela interação doença periodontal e endodôntica e são patologias de difícil diagnóstico clínico. Seu tratamento consiste na terapia endodôntica, terapia periodontal ou ambas. Os tratamentos empregados para as lesões são: Lesões endodônticas primárias: apenas tratamento endodôntico. Lesão endodôntica primária com envolvimento periodontal secundário: primeiramente tratamento endodôntico e após periodontal. Lesão periodontal primária: apenas tratamento periodontal. Lesão periodontal primária com envolvimento endodôntico secundário: primeiramente endodôntico, na sequência periodontal. Lesões verdadeiras combinadas: necessidade de tratamento endodôntico inicial e em seguida periodontal. Uma vez que o tratamento endodôntico tenha sido eficiente, o prognóstico do tratamento dessas lesões depende da severidade da doença periodontal e da resposta dos tecidos periodontais ao tratamento (AU)


The objective of this literature review is to verify the strategies of treatment of the endo-periodontal lesions. A literature search was conducted in the following databases: Medline, Crochane, PubMed and Scielo, containing scientific articles of the last ten years in the English and Portuguese languages using the search terms: "Treatment" AND/OR "Periodontal-endodontic lesions" AND/OR "Periodontal diseases" AND/ OR "endodontic". Endo-periodontal lesions are characterized by the interaction of periodontal and/ or endodontic disease. These are pathologies difficult to diagnose clinically. Their treatment consists of endodontic therapy, periodontal therapy or both. The treatments used for the lesions are: Primary endodontic lesions: only endodontic treatment. Primary endodontic lesion with secondary periodontal involvement: Firstly endodontic treatment and after periodontal treatment. Periodontal lesion primary: only periodontal treatment. Primary periodontal lesion with secondary endodontic involvement: Firstly endodontic, in the periodontal sequence. True true lesions combined: Need for initial and then periodontal endodontic treatment. Since endodontic treatment has been effective, the prognosis of treatment of these complex lesions depends on the severity of periodontal disease and the response of periodontal tissues to treatment (AU)


Assuntos
Doenças Periodontais , Regeneração Óssea , Transplante Ósseo , Endodontia
20.
Braz Dent J ; 29(5): 459-464, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30517444

RESUMO

The aim of this study was to compare the efficacy of grape seed extract (GSE), calcium hypochlorite [Ca(ClO)2], and sodium hypochlorite (NaOCl) irrigant solutions with rotary or reciprocating instrumentation for disinfection of root canals inoculated with Enterococcus faecalis. The mesiobuccal root canals of mandibular molars were prepared and inoculated with Enterococcus faecalis for 21 days. The roots were then randomly divided into the following eight experimental groups (n=11) according to the instrumentation technique and disinfection protocol: ProTaper Next or Reciproc R25 with sodium chloride (control group), 6% NaOCl, 6% Ca(ClO)2, or 50% GSE used for irrigation during instrumentation. The antimicrobial activity was determined on the basis of a reduction in colony-forming units (CFUs) counted on bacterial samples collected before and after root canal instrumentation and expressed as a percentage of reduction. Data were evaluated by two-way ANOVA followed by Tukey HSD post-hoc tests (p<0.05). No significant differences were observed in bacterial reduction between the ProTaper Next and Reciproc R25 systems (p>0.05), regardless of the irrigant solution used. Furthermore, all active solutions (6% NaOCl, 50% GSE, and 6% Ca(ClO)2) showed similar potential to reduce bacterial counts (p>0.05) and were significantly more effective than sodium chloride (control) (p<0.05). The results suggest that the GSE and Ca(ClO)2 have potential clinical application as irrigant solutions in endodontic therapy since they present bactericidal efficacy against Enterococcus faecalis.


Assuntos
Antibacterianos/farmacologia , Desinfecção/métodos , Enterococcus faecalis/efeitos dos fármacos , Irrigantes do Canal Radicular/farmacologia , Compostos de Cálcio/farmacologia , Resinas Compostas/química , Instrumentos Odontológicos , Cavidade Pulpar/microbiologia , Extrato de Sementes de Uva/farmacologia , Humanos , Técnicas In Vitro , Dente Molar , Hipoclorito de Sódio/farmacologia , Células-Tronco
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