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1.
Neurosurg Rev ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32040777

RESUMO

This study aimed to establish an effective prognostic nomogram for microvascular decompression (MVD)-treated trigeminal neuralgia (TN). The nomogram was based on a retrospective cohort study of 1054 patients with TN. During the period 2005-2014, 845 patients at our department treated TN with MVD and served as a development cohort. The predictive accuracy and discriminative ability of the nomogram were determined by concordance index (C-index) and calibration curve. The model was externally validated by 209 TN patients during 2014-2016. Multivariate cox analysis suggested that the patient's age, atypical pain, vascular type, number of offending vessels, and second MVD were significant factors influencing the prognosis of MVD-treated TN. The C index of nomogram in the development cohort was 0.767 (95% CI, 0.739-0.794), and 0.749 (95% CI, 0.688-0.810) in the validation cohort. We developed and validated a nomogram to predict 3-year overall remission rate after MVD treatment of TN. The nomogram can be used in clinical trials to determine the likelihood of pain recurrence in TN patients treated with MVD for 3 years to aid in the comprehensive treatment of TN.

2.
Bioorg Med Chem Lett ; 30(1): 126781, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31706669

RESUMO

In this article, a new compound H2[{Cu(HL)(H2O)}2(P2Mo5O23)]·5H2O (1) (HL = 2-acetylpyrazine thiosemicarbazone) has been synthesized and structurally characterized by single-crystal X-ray diffraction of and other detection techniques. Interestingly, the structure of 1 is different from many reported copper-based complexes, in which the [P2Mo5O23]6-, two Cu2+ ions and two HL were directly connected by covalent bands. Biological studies demonstrated that 1 indicated moderate antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), and a better cytotoxicity against human hepatic cancer line (SMMC-7721) than Mitoxantrone (Mito), the current clinical anticancer drug. Besides, the antibacterial mechanisms of 1 have been studied by the membrane integrity disruption, the destructive reactive oxygen species generation (ROS), the glutathione (GSH) depletion and the depressed enzymatic activity of respiratory chain dehydrogenases (RCD). These results revealed that the combination of HL, Cu2+, [P2Mo5O23]6- shows a higher antibacterial and cytotoxic activity.

3.
Int J Mol Sci ; 20(20)2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31623239

RESUMO

Light is one of the most important abiotic factors for most plants, which affects almost all growth and development stages. In this study, physiological indicators suggest that the application of exogenous Ca2+ improves photosynthesis and changes phytohormone levels. Under weak light, photosynthetic parameters of the net photosynthetic rate (PN), stomatal conductance (Gs), and transpiration rate (Tr) decreased; the antioxidation systems peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) reduced; the degrees of malondialdehyde (MDA), H2O2, and superoxide anion (O2-) free radical damage increased; while exogenous Ca2+ treatment was significantly improved. RNA-seq analysis indicated that a total of 13,640 differently expressed genes (DEGs) were identified and 97 key DEGs related to hormone, photosynthesis, and calcium regulation were differently transcribed. Gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses, plant hormone signal transduction, photosynthesis, carbon metabolism, and phenylpropanoid biosynthesis were significantly enriched. Additionally, quantitative real-time PCR (qRT-PCR) analysis confirmed some of the key gene functions in response to Ca2+. Overall, these results provide novel insights into the complexity of Ca2+ to relieve injuries under weak light, and they are helpful for potato cultivation under weak light stress.

4.
J Craniofac Surg ; 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31609955

RESUMO

OBJECTIVE: Trigeminal neuralgia (TN) is a common cranial nerve disease. Inflammation is suggested in many recent studies to be involved in neuropathic pain, but its role in TN remains unclear so far. Therefore, the current study aimed to explore the relationship of inflammation with TN. METHODS: The levels of inflammatory markers, such as white blood cell (WBC), neutrophil (NE), lymphocyte (LY), monocyte (MO), platelet (PLT), and albumin (ALB), as well as the neutrophil/lymphocyte ratio (NLR), derived NLR (dNLR), platelet/lymphocyte ratio (PLR), monocyte/lymphocyte ratio (MLR), and prognostic nutritional index (PNI) had been compared between TN patients and healthy controls using nonparametric tests. Moreover, multiple logistic regression models had been employed to assess the associations of inflammatory markers with TN. Besides, the receiver operating characteristic (ROC) curve was plotted to analyze the values of these inflammatory makers, as well as their matched combinations in diagnosing TN. RESULTS: The levels of WBC, NE, MO, NLR, dNLR, and MLR in TN patients were evidently increased combined with those in normal subjects. In addition, multivariate logistic regression models illustrated that inflammation had close correlation with TN. Meanwhile, the area under the curve (AUC) values for NE, NLR, and dNLR, as well as those for the matched combinations of NLR+PLR, NLR+PNI, dNLR+NLR, and dNLR+PLR in TN were >0.7, which might have predictive value for TN compared with those for normal subjects. CONCLUSIONS: Findings of this study reveal that inflammation could have played a close and important role in the progression and etiology of TN.

5.
Molecules ; 24(17)2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31466314

RESUMO

The magnetic adsorbent, Fe3O4@[Ni(HL)2]2H2[P2Mo5O23]·2H2O (Fe3O4@1), is synthesized by employing the nanoparticles Fe3O4 and polyoxometalate hybrid 1. Zero-field-cooled (ZFC) and field-cooled (FC) curves show that the blocking temperature of Fe3O4@1 was at 120 K. Studies of Fe3O4@1 removing cationic and anionic dyes from water have been explored. The characterization of Fe3O4@1, effects of critical factors such as dosage, the concentration of methylene blue (MB), pH, adsorption kinetics, isotherm, the removal selectivity of substrate and the reusability of Fe3O4@1 were assessed. The magnetic adsorbent displayed an outstanding removal activity for the cationic dye at a broad range of pH. The adsorption kinetics and isotherm models revealed that the adsorption process of Fe3O4@1 was mainly governed via chemisorption. The maximum capacity of Fe3O4@1 adsorbing substance was 41.91 mg g-1. Furthermore, Fe3O4@1 showed its high stability by remaining for seven runs of the adsorption-desorption process with an effective MB removal rate, and could also be developed as a valuable adsorbent for dyes elimination from aqueous system.


Assuntos
Corantes/análise , Compostos Ferrosos/química , Compostos de Tungstênio/química , Adsorção , Concentração de Íons de Hidrogênio , Estrutura Molecular , Nanopartículas , Purificação da Água
6.
Childs Nerv Syst ; 35(12): 2385-2389, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31289856

RESUMO

OBJECTIVES: Cluster headache is a primary headache disorder, which has affected up to 0.1% population. Superficial temporal artery ligation combined with auriculotemporal nerve transection (SLAT) is one of the surgical alternatives to treat the drug-resistant temporal cluster headache (TCH). The current work aimed to assess the effect of SLAT on TCH patients based on the very long-term clinical follow-up. METHODS: The current retrospective study had enrolled 20 adolescent TCH patients undergoing SLAT between December 2016 and January 2018. The headache diaries as well as the pain severity questionnaire of the visual analog scale (VAS) had been collected to measure the pain severity before and after surgery. RESULTS: The pain-free rates 3 days, as well as 1, 6, and 12 months, after SLAT surgery were 2.00%, 10.00%, 25.00%, and 70.00%, respectively. The frequency of TCH attack daily was found to be markedly reduced on the whole; besides, the pain degree was also remarkably decreased. CONCLUSIONS: Results in this study indicate that the sustained headache can be relieved after SLAT in adolescent patients with intractable TCH.

7.
World Neurosurg ; 128: e556-e561, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31071444

RESUMO

OBJECTIVE: Trigeminal neuralgia (TN) is a common cranial nerve disease. Meanwhile, it is suggested in some studies that orofacial pain can also lead to some psychological diseases. Therefore, the current study was carried out aiming to explore the relationship between depression as well as anxiety and TN; at the same time, the effect on the postoperative outcome of microvascular decompression (MVD) would also be explored. METHODS: The Hamilton Depression Rating Scale (HDRS) scores, as well as the Hamilton Anxiety Rating Scale (HARS) scores in TN cases were compared with those among patients without TN. Multiple logistic regression models were also used to assess the associations of HDRS and HARS scores with TN. Patients were divided into 2 groups according to the MVD outcome in TN patients, and the HDRS and HARS scores were between pain-free patients and those who still suffered from pain. RESULTS: The HDRS and HARS scores in TN patients were evidently increased relative to those observed in normal individuals. HDRS and HARS scores were found to be positively associated with the Visual Analog Scale pain score and onset duration in TN patients. Additionally, remarkably higher HDRS and HARS scores were observed in the persistent pain group than in pain-free group. CONCLUSIONS: The findings of this study reveal that depression and anxiety are closely associated with the incidence of TN, which may also affect the outcome of patients undergoing MVD.


Assuntos
Ansiedade/psicologia , Depressão/psicologia , Cirurgia de Descompressão Microvascular , Neuralgia do Trigêmeo/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Ansiedade/epidemiologia , Comorbidade , Depressão/epidemiologia , Dor Facial/epidemiologia , Dor Facial/psicologia , Dor Facial/cirurgia , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Medição da Dor , Resultado do Tratamento , Neuralgia do Trigêmeo/epidemiologia , Neuralgia do Trigêmeo/psicologia
8.
J Craniofac Surg ; 30(6): e556-e558, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30939556

RESUMO

BACKGROUND AND OBJECTIVE: Trigeminal neuralgia (TN) is a common cranial nerve disease. Uric acid (URIC), a water-soluble antioxidant discovered in human body, has been recognized in numerous recent studies to exert a crucial part in neuroprotection; however, the influence of URIC on TN remains unclear so far. This study aimed to examine the association of URIC with TN. METHODS: From January 2017 to September 2018, medical records from the newly diagnosed patients with TN at the Xinhua Hospital were retrospectively recruited and analyzed. The serum URIC, creatinine, blood urea nitrogen, and albumin levels between TN patients and normal subjects were compared through the nonparametric tests. Moreover, the relationship of URIC levels with TN was assessed using the multiple linear regression models. RESULTS: Compared with normal subjects (325.7 ±â€Š74.3 µmol/L), URIC contents were remarkably decreased in TN patients (270.2 ±â€Š75.9 µmol/L) (P < 0.05). Besides, URIC was regarded as a protective factor of TN, as verified by multivariate logistic regression models (odds ratio = 0.2, 95% confidence interval = 0.0-0.6; P < 0.05). CONCLUSION: Low URIC content is associated with the risk of incidence of TN, and appropriately increasing the URIC level may prevent TN.

9.
Childs Nerv Syst ; 34(2): 321-327, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-28921161

RESUMO

OBJECTIVE: Conflicting conclusions have been reported regarding predictors of deep brain stimulation (DBS) outcome in patients with refractory temporal lobe epilepsy (TLE). The main goal of this meta-analysis study was to identify possible predictors of remarkable seizure reduction (RSR). METHODS: We conducted a comprehensive search of English-language literature published since 1990 and indexed in PubMed, Embase, and the Cochrane Library that addressed seizure outcomes in patients who underwent DBS for refractory TLE. A pooled RSR rate was determined for eight included studies. RSR rates were analyzed relative to potential prognostic variables. Random- or fixed-effects models were used depending on the presence or absence of heterogeneity. RESULTS: The pooled RSR rate among 61 DBS-treated patients with TLE from 8 studies was 59%. Higher likelihood of RSR was found to be associated with lateralization of stimulation, lateralized ictal EEG findings, and a longer follow-up period. Seizure semiology, MRI abnormalities, and patient sex were not predictive of RSR rate. The best electrode type for RSR was the Medtronic 3389. Hippocampal and anterior thalamic nuclei (ATN) sites of stimulation had similar odds of producing RSR. CONCLUSIONS: DBS is an effective therapeutic modality for intractable TLE, particularly in patients with lateralized EEG abnormalities and in patients treated on the ictal side. This meta-analysis provides evidence-based information for determining DBS suitability in presurgical counseling and for explaining seizure outcomes.


Assuntos
Estimulação Encefálica Profunda/métodos , Epilepsia Resistente a Medicamentos/cirurgia , Epilepsia do Lobo Temporal/cirurgia , Convulsões/prevenção & controle , Estimulação Encefálica Profunda/tendências , Epilepsia Resistente a Medicamentos/diagnóstico , Epilepsia Resistente a Medicamentos/epidemiologia , Epilepsia do Lobo Temporal/diagnóstico , Epilepsia do Lobo Temporal/epidemiologia , Humanos , Convulsões/diagnóstico , Convulsões/epidemiologia , Resultado do Tratamento
10.
Oncotarget ; 8(61): 104216-104226, 2017 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-29262634

RESUMO

Advanced glycation end products (AGEs) have been reported to take part in many cancer processes. Whether AGEs contribute to gastric cancer (GC) course and the underlying mechanism are still unclear. Here, glucose-derived AGEs are detected to be accumulated in tumor tissues and blood of patients with GC. As the receptor for AGEs, RAGE is highly expressed in cancer tissues, and closely associated with the depth of cancer invasion, lymph node metastasis and TNM stage. Both in vivo and in vitro treatment of AGEs accelerate the tumor invasion and metastasis, with upregualtion of RAGE, Specificity Protein 1 (Sp1), and MMP2 protein expression, as well as enhancement of MMP2 activity. Either RAGE-blocking antibody or Sp1-knockdown can partially block the AGEs-induced effects. Moreover, AGEs increased the phosphorylation of ERK, and reducing the phosphorylation level of ERK by MEK1/2 inhibitor decreased the expression of Sp1. These results indicate that accumulation of glucose-derived AGEs may act as one of potential risk factors for GC progression and promote the invasion and metastasis of gastric cancer partially through the activation of RAGE/ERK/Sp1/MMP2 pathway.

11.
Biomed Res Int ; 2016: 3708187, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27314017

RESUMO

We selected different concentrations of ethephon, to stress C. roseus. We used qRT-PCR and HPLC followed by PCA to obtain comprehensive profiling of the vinblastine biosynthesis in response to ethephon. Based on our findings, the results showed that the high concentration of ethephon had a positive effect at both transcriptional and metabolite level. Meanwhile, there was a remarkable decrease of hydrogen peroxide content and a promoted peroxidase activity in leaves. The loading plot combination with correlation analysis suggested that CrPrx1 could be regarded as a positive regulator and interacts with ethylene response factor (ERF) to play a key role in vinblastine content and peroxidase (POD) activity. This study provides the foundation for a better understanding of the regulation and accumulation of vinblastine in response to ethephon.


Assuntos
Catharanthus/genética , Etilenos/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Genes de Plantas/genética , Vimblastina/metabolismo , Catharanthus/metabolismo , Etilenos/análise , Compostos Organofosforados/farmacologia , Oxirredução , Vimblastina/análise
12.
Front Plant Sci ; 6: 1203, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26779234

RESUMO

The gas molecule nitric oxide (NO) can cooperate with ethylene to tightly modulate plant growth and stress responses. One of the mechanism of their crosstalk is that NO is able to activate ethylene biosynthesis, possibly through post-translational modification of key enzymes such as ACC synthase and oxidase by S-nitrosylation. In this paper, we focus on the crosstalk of NO with ethylene signaling transduction transcription factor EIN3 (Ethylene Insensitive 3) and downstream gene expression in alleviating germination inhibition and growth damage induced by high salt. The Arabidopsis lines affected in ethylene signaling (ein3eil1) and NO biosynthesis (nia1nia2) were employed to compare with the wild-type Col-0 and overexpressing line EIN3ox. Firstly, the obviously inhibited germination, greater ratio of bleached leaves and enhanced electrolyte leakage were found in ein3eil1 and nia1nia2 lines than in Col-0 plants upon high salinity. However, the line EIN3ox obtained a notably elevated ability to germinate and improved seedling resistance. The experiment with SNP alone or plus high salt mostly enhanced the expression of EIN3 transcripts, compared with ACO4 and ACS2. The western blot and transcript analysis found that high-salt-induced EIN3 stabilization and EIN3 transcripts were largely attenuated in the NO biogenesis mutant nia1nia2 plants than in Col-0 ones. This observation was confirmed by simulation experiments with NO scavenger cPTIO to block NO emission. Taken together, our study provides insights that NO promotes seed germination and seedlings growth under salinity may depend on EIN3 protein.

13.
Plant Physiol Biochem ; 77: 140-8, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24589477

RESUMO

The effects of exogenous trehalose (Tre) on salt tolerance of pharmaceutical plant Catharanthus roseus and the physiological mechanisms were both investigated in this study. The results showed that the supplement of Tre in saline condition (250 mM NaCl) largely alleviated the inhibitory effects of salinity on plant growth, namely biomass accumulation and total leaf area per plant. In this saline condition, the decreased level of relative water content (RWC) and photosynthetic rate were also greatly rescued by exogenous Tre. This improved performance of plants under high salinity induced by Tre could be partly ascribed to its ability to decrease accumulation of sodium, and increase potassium in leaves. The exogenous Tre led to high levels of fructose, glucose, sucrose and Tre inside the salt-stressed plants during whole the three-week treatment. The major free amino acids such as proline, arginine, threonine and glutamate were also largely elevated in the first two-week course of treatment with Tre in saline solution. It was proposed here that Tre might act as signal to make the salt-stressed plants actively increase internal compatible solutes, including soluble sugars and free amino acids, to control water loss, leaf gas exchange and ionic flow at the onset of salt stress. The application of Tre in saline condition also promoted the accumulation of alkaloids. The regulatory role of Tre in improving salt tolerance was optimal with an exogenous concentration of 10 mM Tre. Larger concentrations of Tre were supra-optimum and adversely affected plant growth.


Assuntos
Catharanthus/efeitos dos fármacos , Íons/metabolismo , Osmorregulação/efeitos dos fármacos , Salinidade , Tolerância ao Sal/efeitos dos fármacos , Cloreto de Sódio/efeitos adversos , Trealose/farmacologia , Alcaloides/metabolismo , Aminoácidos/metabolismo , Biomassa , Metabolismo dos Carboidratos/efeitos dos fármacos , Catharanthus/metabolismo , Osmose , Pressão Osmótica , Fotossíntese , Folhas de Planta/metabolismo , Potássio/metabolismo , Sódio/metabolismo , Cloreto de Sódio/metabolismo , Estresse Fisiológico/efeitos dos fármacos , Trealose/metabolismo , Água/metabolismo
14.
Med Oncol ; 30(2): 595, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23645556

RESUMO

Notch3 receptor is one of the mammalian Notch family receptors (Notch1-4) which plays an important role in the regulation of cellular proliferation, differentiation, and apoptosis. Overexpression of Notch3 is associated with tumorigenesis. In order to assess the expression of Notch3 in Chinese non-small-cell lung cancer (NSCLC) patients and determine its association with prognosis, we designed a prospective study with five years of follow-up to evaluate Notch3 expression in NSCLC tissues and adjacent non-cancerous normal lung tissues from 131 patients undergoing surgical treatment by immunohistochemistry and western blot analysis. Notch3 had high expression in 67 of 131 cases of NSCLC (51.1 %), which was significantly higher than in adjacent noncancerous lung tissues. Moreover, Notch3 overexpression was significantly correlated with TNM stage (P = 5.41e-07 in squamous cell carcinoma, P = 5.338e-07 in adenocarcinoma) and lymph node metastasis (P = 0.00764 in squamous cell carcinoma, P = 0.01491 in adenocarcinoma). Kaplan-Meier survival analysis showed that the overall survival times in patients expressing Notch3 in NSCLC were shorter. Multivariate analysis further demonstrated that Notch3 was an independent prognostic factor for patients with NSCLC. Therefore, Notch3 might be a useful biomarker to predict the prognosis of patients with NSCLC.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Neoplasias Pulmonares/metabolismo , Receptores Notch/biossíntese , Western Blotting , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Carcinoma Pulmonar de Células não Pequenas/genética , Feminino , Humanos , Imuno-Histoquímica , Estimativa de Kaplan-Meier , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/genética , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Prognóstico , Estudos Prospectivos , Receptor Notch3 , Receptores Notch/genética
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