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1.
Life Sci ; 142: 49-59, 2015 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-26475965

RESUMO

AIMS: Propofol (PPF), an intravenous anesthetic agent, is previously reported to attenuate oxidative stress- and inflammation-induced endothelial cell dysfunction. This study investigated its effect on endothelial cell biology. MAIN METHODS: Cultured human umbilical vein endothelial cells (HUVECs) were treated with PPF and subject to measurements for nitric oxide (NO) production, autophagy flux, signal transduction, migration, and in vitro angiogenesis. KEY FINDINGS: Non-cytotoxic PPF treatment was found to significantly upregulate inducible nitric oxide synthase (NOS2) but downregulate constitutive NOS3 expression. It also potentiated LPS-induced ICAM-1 overexpression and NO overproduction. Mechanistically, the PPF-activated signal transduction in PI3K/Akt, ERK1/2, p38 MAPK, and JNK pathways were involved in the PPF-driven NO overproduction. PPF exhibited a stimulatory effect on autophagy flux by increasing expression of autophagy markers including mTOR, Beclin-1, Atg5, and LC3I/II, as well as a late endosomal indicator, Rab7. However, PPF appeared to antagonize the Rab7 upregulation by LPS. Functionally, PPF enhanced in vitro migratory and angiogenic capacities of HUVECs, but this enhancement was drastically abrogated by the presence of autophagy inhibitors, indicating a pro-angiogenic contribution of PPF-enhanced autophagy in cultured HUVECs. SIGNIFICANCE: Our findings support the notion that PPF enhances motility and angiogenic capacity of cultured HUVECs through an autophagy-involved regulatory mechanism.


Assuntos
Autofagia/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/metabolismo , Neovascularização Fisiológica/efeitos dos fármacos , Propofol/farmacologia , Transdução de Sinais/efeitos dos fármacos , Células Cultivadas , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/citologia , Humanos , Óxido Nítrico/biossíntese , Óxido Nítrico Sintase Tipo III/biossíntese
2.
Cancer Lett ; 368(1): 144-152, 2015 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-26276725

RESUMO

Toll-like receptor-4 (TLR4) is known to influence growth and migration of hepatocellular tumors; however, its role in hepatoblastoma remains poorly understood. This study investigated the regulatory role of TLR4 in proliferation and chemoresistance of HepG2 hepatoblastoma cells. Treatment with lipopolysaccharide (LPS), a TLR4 agonist, was found to significantly upregulate TLR4 expression in HepG2 cells, but not in malignant Huh-7 and Sk-Hep1 hepatocellular carcinoma cells. Additionally, IL-6 enhanced LPS-induced TLR4 upregulation. LPS-stimulated TLR4 activation increased proliferation, nitric oxide synthase (NOS) expression, and NO production in HepG2 cells. Chemotherapeutic agents, cisplatin and doxorubicin, effectively inhibited TLR4 expression in HepG2 cells. Characterization of LPS-induced signaling activation and blockade with kinase inhibitors revealed the involvement of Akt and MAPK pathways in LPS-enhanced NO release from, and proliferation of HepG2 cells. Mechanistically, gene modifications as a result of TLR4 transfection and siRNA-mediated knockdown further demonstrated a crucial role for TLR4 in the regulation of NOS expression, cell proliferation, and chemoresistance in HepG2 cells. These findings suggest that targeting TLR4 expression and its cognate signaling may modulate proliferation and chemosensitivity in hepatoblastoma cells and serve as a potential therapeutic target.


Assuntos
Proliferação de Células/efeitos dos fármacos , Cisplatino/farmacologia , Doxorrubicina/farmacologia , Resistencia a Medicamentos Antineoplásicos , Hepatoblastoma/metabolismo , Lipopolissacarídeos/farmacologia , Neoplasias Hepáticas/metabolismo , Receptor 4 Toll-Like/efeitos dos fármacos , Relação Dose-Resposta a Droga , Técnicas de Silenciamento de Genes , Células Hep G2 , Hepatoblastoma/genética , Hepatoblastoma/patologia , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/patologia , Proteínas Quinases Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores , Proteínas Proto-Oncogênicas c-akt/metabolismo , Interferência de RNA , Transdução de Sinais/efeitos dos fármacos , Fatores de Tempo , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/metabolismo , Transfecção
3.
Biochim Biophys Acta ; 1842(9): 1720-32, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24970745

RESUMO

This study investigated the pro-fibrogenic role of high mobility group box 1 (HMGB1) peptides in liver fibrogenesis. An animal model of carbon tetrachloride (CCl4)-induced liver fibrosis was used to examine the serum HMGB1 levels and its intrahepatic distribution. The increased serum HMGB1 levels were positively correlated with elevation of transforming growth factor-ß1 (TGF-ß1) and collagen deposition during fibrogenesis. The cytoplasmic distribution of HMGB1 was noted in the parenchymal hepatocytes of fibrotic livers. In vitro studies confirmed that exposure to hydrogen peroxide and CCl4 induced an intracellular mobilization and extracellular release of nuclear HMGB1 peptides in clone-9 and primary hepatocytes, respectively. An uptake of exogenous HMGB1 by hepatic stellate cells (HSCs) T6 cells indicated a possible paracrine action of hepatocytes on HSCs. Moreover, HMGB1 dose-dependently stimulated HSC proliferation, up-regulated de novo synthesis of collagen type I and α-smooth muscle actin (α-SMA), and triggered Smad2 phosphorylation and its nuclear translocation through a TGF-ß1-independent mechanism. Blockade with neutralizing antibodies and gene silencing demonstrated the involvement of the receptor for advanced glycation end-products (RAGE), but not toll-like receptor 4, in cellular uptake of HMGB1 and the HMGB1-mediated Smad2 and ERK1/2 phosphorylation as well as α-SMA up-regulation in HSC-T6 cells. Furthermore, anti-RAGE treatment significantly ameliorated CCl4-induced liver fibrosis. In conclusion, the nuclear HMGB1 peptides released from parenchymal hepatocytes during liver injuries may directly activate HSCs through stimulating HSC proliferation and transformation, eventually leading to the fibrotic changes of livers. Blockade of HMGB1/RAGE signaling cascade may constitute a therapeutic strategy for treatment of liver fibrosis.


Assuntos
Núcleo Celular/metabolismo , Proteína HMGB1/metabolismo , Células Estreladas do Fígado/patologia , Hepatócitos/patologia , Cirrose Hepática/patologia , Fragmentos de Peptídeos/farmacologia , Animais , Western Blotting , Tetracloreto de Carbono/toxicidade , Proliferação de Células , Células Cultivadas , Citoplasma/metabolismo , Proteína HMGB1/genética , Células Estreladas do Fígado/efeitos dos fármacos , Células Estreladas do Fígado/metabolismo , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Peróxido de Hidrogênio/farmacologia , Técnicas Imunoenzimáticas , Imunoprecipitação , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos ICR , Oxidantes/farmacologia , RNA Mensageiro/genética , RNA Interferente Pequeno/genética , Reação em Cadeia da Polimerase em Tempo Real , Receptor para Produtos Finais de Glicação Avançada , Receptores Imunológicos/antagonistas & inibidores , Receptores Imunológicos/genética , Receptores Imunológicos/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Receptor 4 Toll-Like/antagonistas & inibidores , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/metabolismo
7.
Biomed Microdevices ; 13(1): 89-95, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20830527

RESUMO

The failure of tumor stents used for palliative therapy is due in part to the adhesion of tumor cells to the stent surface. It is therefore desirable to develop approaches to weaken the adhesion of malignant tumor cells to surfaces. We have previously developed SiO2 coated nanorods that resist the adhesion of normal endothelial cells and fibroblasts. The adhesion mechanisms in malignant tumor cells are significantly altered from normal cells; therefore, it is unclear if nanorods can similarly resist tumor cell adhesion. In this study, we show that the morphology of tumor epithelial cells cultured on nanorods is rounded compared to flat surfaces and associated with decreased cellular stiffness and non-muscle myosin II phosphorylation. Tumor cell viability and proliferation was unchanged on nanorods. Adherent cell numbers were significantly decreased while single tumor cell motility was increased on nanorods compared to flat surfaces. Together, these results suggest that nanorods can be used to weaken malignant tumor cell adhesion, and therefore potentially improve tumor stent performance.


Assuntos
Carcinoma/patologia , Movimento Celular , Fenômenos Mecânicos , Nanotubos , Fenômenos Biomecânicos , Carcinoma/metabolismo , Carcinoma/terapia , Adesão Celular , Contagem de Células , Linhagem Celular Tumoral , Humanos , Miosina Tipo II/metabolismo , Stents , Propriedades de Superfície
8.
Sensors (Basel) ; 9(6): 4669-94, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-22408548

RESUMO

In this paper, we review our recent results in developing gas sensors for hydrogen using various device structures, including ZnO nanowires and GaN High Electron Mobility Transistors (HEMTs). ZnO nanowires are particularly interesting because they have a large surface area to volume ratio, which will improve sensitivity, and because they operate at low current levels, will have low power requirements in a sensor module. GaN-based devices offer the advantage of the HEMT structure, high temperature operation, and simple integration with existing fabrication technology and sensing systems. Improvements in sensitivity, recoverability, and reliability are presented. Also reported are demonstrations of detection of other gases, including CO(2) and C(2)H(4) using functionalized GaN HEMTs. This is critical for the development of lab-on-a-chip type systems and can provide a significant advance towards a market-ready sensor application.

9.
J Nanosci Nanotechnol ; 8(1): 99-110, 2008 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18468056

RESUMO

A brief review is given of recent developments in wide bandgap semiconductor nanowire synthesis and devices fabricated on these nanostructures. There is strong interest in these devices for applications in UV detection, gas sensors and transparent electronics.

10.
Proteomics ; 7(17): 3038-50, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17676666

RESUMO

Development of a rapid, effective, and highly specific platform for target identification in complex biofluids is one of the most important tasks in proteomic research. Taking advantage of the natural hydrophobic interaction of PVDF with probe protein, a simple and effective method was developed for protein quantitation and profiling. Using antibody-antigen interactions as a proof-of-concept system, the targeted plasma proteins, serum amyloid P (SAP), serum amyloid A (SAA), and C-reactive protein (CRP), could be selectively isolated and enriched from human plasma by antibody-immobilized PVDF membrane and directly identified by MALDI-TOF MS without additional elution step. The approach was successfully applied to human plasma for rapid quantitation and variant screening of SAP, SAA, and CRP in healthy individuals and patients with gastric cancer. The triplexed on-probe quantitative analysis revealed significant overexpression of CRP and SAA in gastric cancer group, consistent with parallel ELISA measurements and pathological progression and prognostic significance reported in previous literatures. Furthermore, the variant mass profiling of the post-translationally modified forms revealed a high occurrence of de-sialic acid SAP in patients with gastric cancer. Due to the versatile assay design, ease of probe preparation without chemical synthesis, and compatibility with MALDI-TOF MS analysis, the methodology may be useful for target protein characterization, functional proteomics, and screening in clinical proteomics.


Assuntos
Proteína C-Reativa/análise , Polivinil/química , Proteína Amiloide A Sérica/análise , Componente Amiloide P Sérico/análise , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Proteína C-Reativa/isolamento & purificação , Proteína C-Reativa/metabolismo , Estudos de Casos e Controles , Ensaio de Imunoadsorção Enzimática , Humanos , Membranas Artificiais , Modelos Biológicos , Prognóstico , Processamento de Proteína Pós-Traducional , Proteômica/métodos , Proteína Amiloide A Sérica/isolamento & purificação , Proteína Amiloide A Sérica/metabolismo , Componente Amiloide P Sérico/isolamento & purificação , Componente Amiloide P Sérico/metabolismo , Neoplasias Gástricas/sangue , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologia
11.
Fertil Steril ; 85 Suppl 1: 1195-203, 2006 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-16616092

RESUMO

OBJECTIVE: To investigate the effects of androgen and antiandrogen and the expression of androgen receptor on mouse embryonic stem cells (ESCs) and the inner cell mass. DESIGN: Controlled laboratory study. SETTING: Academic university hospital. ANIMAL(S): Blastocysts from mice developed at the Institute for Cancer Research and 129/Sv mice embryonic stem cell line. INTERVENTION(S): Cultured mouse ESCs were exposed to testosterone (T), dihydrotestosterone (DHT), or the antiandrogen nilutamide. MAIN OUTCOME MEASURE(S): Immunohistochemistry for androgen receptor (AR), quantitative real-time polymerase chain reaction analysis, cell colorimetric assays, and Western blot analysis. RESULT(S): Androgen receptor messenger RNA (mRNA) was first detected both in the inner cell mass from blastocysts and in undifferentiated ESCs. It increased stage-dependently during ESC differentiation. Although both T and DHT had marginal effects on AR mRNA expression level and cell growth in vitro, the nonsteroidal antiandrogen nilutamide significantly stimulated ESC growth and induced Akt expression. The enhancing effects of nilutamide on mouse ESCs indicated that the Akt pathway may be involved in nilutamide-promoted ESC growth. CONCLUSION(S): These findings provide the first evidence of the existence of AR in ESCs. During differentiation, the expression level of AR was increased in a stage-dependent but not a ligand-dependent manner. Nilutamide promoted cell growth and increased Akt expression in ESCs.


Assuntos
Antagonistas de Androgênios/farmacologia , Androgênios/administração & dosagem , Imidazolidinas/farmacologia , Receptores Adrenérgicos/metabolismo , Células-Tronco/citologia , Células-Tronco/metabolismo , Animais , Blastocisto/citologia , Blastocisto/efeitos dos fármacos , Blastocisto/metabolismo , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Di-Hidrotestosterona/farmacologia , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Camundongos , Camundongos Endogâmicos ICR , Receptores Adrenérgicos/efeitos dos fármacos , Células-Tronco/efeitos dos fármacos , Testosterona/farmacologia
12.
Anal Chim Acta ; 556(1): 237-46, 2006 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-17723354

RESUMO

Characterization of membrane proteins remains an analytical challenge because of difficulties associated with tedious isolation and purification. This study presents the utility of the polyvinylidene difluoride (PVDF) membrane for direct sub-proteome profiling and membrane protein characterization by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The hydrophobic adsorption of protein, particularly membrane proteins, on the PVDF surface enables efficient on-PVDF washing to remove high concentrations of detergents and salts, such as up to 5% sodium dodecyl sulfate (SDS). The enhanced spectrum quality for MALDI detection is particularly notable for high molecular weight proteins. By using on-PVDF washing prior to MALDI detection, we obtained protein profiles of the detergent-containing and detergent-insoluble membrane fractions from Methylococcus capsulatus (Bath). Similar improvements of signal-to-noise ratios were shown on the MALDI spectra for proteins electroblotted from SDS-polyacrylamide gel electrophoresis (SDS-PAGE) onto the PVDF membrane. We have applied this strategy to obtain intact molecular weights of the particulate methane monooxygenase (pMMO) composed of three intrinsic membrane-bound proteins, PmoA, PmoB, and PmoC. Together with peptide sequencing by tandem mass spectrometry, post-translational modifications including N-terminal acetylation of PmoA and PmoC and alternative C-terminal truncation of PmoB were identified. The above results show that PVDF-aided MALDI-MS can be an effective approach for profiling and characterization of membrane proteins.

13.
J Assist Reprod Genet ; 22(5): 191-8, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-16047580

RESUMO

PURPOSE: The purpose of this study was to evaluate the influences of tropical weather on patients with different ovarian responses in the treatment of assisted reproductive technology. METHODS: Six-hundred fourty-seven women underwent their first treatment cycles were retrospectively analyzed. Patients received embryo transfer either 3 days or 5 days after oocyte retrieval, depending on the number and quality of embryos on day-2. RESULTS: Significant correlations were demonstrated in the top quality embryo rates of day-3 and day-5 embryo transfers with temperature, humidity, and atmosphere pressure. The cumulative light hours negatively correlated with the implantation and pregnancy rates of day-3 embryo transfer (-.282 and -.282, respectively), while they positively correlated with those of day-5 embryo transfer (.225 and .224, respectively). CONCLUSIONS: These results clearly suggest that weather may exert influences on the outcome of assisted reproductive technology. Patients with different ovarian responses or blastocyst culture and transfer may modify weather influences.


Assuntos
Implantação do Embrião/fisiologia , Transferência Embrionária , Indução da Ovulação/métodos , Injeções de Esperma Intracitoplásmicas , Tempo (Meteorologia) , Adulto , Feminino , Humanos , Masculino , Gravidez , Estudos Retrospectivos , Estações do Ano , Taiwan , Fatores de Tempo , Clima Tropical
15.
Fertil Steril ; 83 Suppl 1: 1241-7, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15831298

RESUMO

OBJECTIVE: To examine expression of androgen receptor (AR), AR cofactors, estrogen (E) receptor alpha, E receptor beta, progesterone receptor, steroid receptor coactivator-1, and aromatase in human luteinized granulosa cells collected during oocyte retrieval. DESIGN: Prospective real-time reverse transcriptase-polymerase chain reaction study. SETTING: Academic medical center. PATIENT(S): A total of 198 samples were brought into the study. INTERVENTION(S): Patients underwent the long protocol for assisted reproductive technology. Luteinized granulosa cells were collected transvaginally with ultrasound guidance. Quantitative reverse transcriptase-polymerase chain reaction was performed to quantify the mRNA expression of the investigated genes. MAIN OUTCOME MEASURE(S): The expression levels were determined as ratios between the studied genes and the reference gene beta-actin. RESULT(S): There is little AR expression in human luteinized granulosa cells immediately preceding ovulation under controlled ovarian hyperstimulation. All aspirated follicles, despite their antral size, displayed a similar mRNA expression of the investigated genes in the luteinized granulosa cells. CONCLUSION(S): This study supports the possibility of a transition of androgen action from being an enhancer of follicular differentiation (through the AR) to being a substrate of E synthesis (through aromatase) at the time of oocyte retrieval. The present study also demonstrates no effect of follicular size upon the status of steroid receptor mRNA expression in the luteinized granulosa cells when follicles were at least >1.5 mL.


Assuntos
Aromatase/genética , Células da Granulosa/fisiologia , Peptídeos e Proteínas de Sinalização Intracelular/genética , Indução da Ovulação , Receptores de Esteroides/genética , Proteínas do Citoesqueleto/genética , Proteínas de Ligação a DNA/genética , Feminino , Fertilização In Vitro , Humanos , Proteínas com Domínio LIM , Modelos Lineares , Coativadores de Receptor Nuclear , Proteínas Oncogênicas/genética , Receptores Androgênicos/genética , Receptores Estrogênicos/genética , Receptores de Progesterona/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Transcrição/genética
16.
Fertil Steril ; 81(6): 1642-9, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15193489

RESUMO

OBJECTIVE: To determine the accuracy of differential diagnosis by team consultation of abortion in progression, low-lying implantation/cervicoisthmic pregnancy, and cervical pregnancy (CP) in patients referred for suspicion of abnormal implantation on the lower segment and cervix of the uterus and to determine the efficacy of endoscopic surgery with uterine artery blockade followed by cervical evacuation in the treatment of confirmed CP. DESIGN: Prospective observational study under multiple-clinic and multiple-hospital cooperation. SETTING: Tertiary clinical and academic medical center. PATIENT(S): Twenty-seven women with a tentative diagnosis of CP made at their primary gynecologists' offices from July 1999 to June 2003. INTERVENTION(S): Second-opinion ultrasound scanning with transabdominal and transvaginal approach and optional color Doppler use. For patients with confirmed CP, a new treatment modality with laparoscopy-assisted uterine artery ligation followed by hysteroscopic local endocervical resection to remove the ectopic pregnancy was employed. For patients with abortion in progression or low-lying implantation/cervicoisthmic pregnancy (non-CP) requiring termination, dilatation and curettage (D&C) was performed under transabdominal ultrasound guidance. MAIN OUTCOME MEASURE(S): Fulfillment of ultrasound-based diagnostic criteria and operative course, convalescence, and commencement of menstruation in those patients with confirmed CP. RESULT(S): Cervical pregnancy was diagnosed in six (22.2%) patients at

Assuntos
Colo do Útero/cirurgia , Histeroscopia , Laparoscopia , Gravidez Ectópica/diagnóstico por imagem , Gravidez Ectópica/cirurgia , Útero/irrigação sanguínea , Aborto Espontâneo/diagnóstico por imagem , Adulto , Artérias , Perda Sanguínea Cirúrgica , Diagnóstico Diferencial , Feminino , Humanos , Ligadura , Menstruação , Período Pós-Operatório , Gravidez , Estudos Prospectivos , Fatores de Tempo , Ultrassonografia Doppler em Cores
17.
J Reprod Med ; 49(5): 345-52, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15214706

RESUMO

OBJECTIVE: To test the safety and efficacy of a modified laser-assisted zonal opening method for human embryo biopsy. STUDY DESIGN: The embryo was treated with a modified method to create an ample perivitelline space between the zona pellucida and underlying blastomeres. This was done to protect the blastomeres from damage by laser treatment of the zona pellucida. Subsequently, the zona pellucida was completely perforated using a diode laser, and the targeted blastomere was aspirated. In vitro embryo development of the 40 biopsied embryos was compared with that of 322 corresponding control embryos without embryo biopsy. RESULTS: Forty targeted blastomeres were successfully extracted from 40 human embryos. The incidence of embryo development to the blastocyst stage was not different between the biopsied and nonbiopsied groups (55% vs. 54.3%). Furthermore, the percentage of top-scoring blastocysts from biopsied embryos was also similar to that of control embryos (77.3% vs. 78.9%). CONCLUSION: These preliminary results demonstrate that the modified technique for human embryo biopsy in preimplantation genetic diagnosis is simple and safe.


Assuntos
Biópsia/métodos , Blastocisto , Terapia a Laser , Diagnóstico Pré-Implantação/métodos , Adulto , Biópsia/efeitos adversos , Blastômeros , Estudos de Coortes , Feminino , Fertilização In Vitro , Humanos , Gravidez , Zona Pelúcida
18.
Hum Reprod ; 19(2): 306-15, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-14747172

RESUMO

BACKGROUND: This study compares the influence of recombinant (r)FSH and urinary (u)FSH stimulation on oocyte and embryo quality in patients undergoing ICSI. METHODS AND RESULTS: Denuded oocyte maturity in ICSI patients was graded on a scale from metaphase II (MII) to prophase for nuclear maturation of oocytes. The relationships of cumulus-free oocyte maturational profiles with in vitro outcome parameters were evaluated. In the study population with an unknown distribution of FSH receptor polymorphisms, the ovarian response to rFSH stimulation was significantly different from that of uFSH stimulation, including lower number of oocytes retrieved/oocytes in MII, higher fertilization rates and higher good quality embryo rates. In the study population with a similar distribution of FSH receptor polymorphisms, the ovarian responses to rFSH were lower numbers of oocytes in MII, higher fertilization rates and higher good quality embryo rates, but the total number of oocytes retrieved was not influenced, in comparison with ovarian stimulation with uFSH. CONCLUSIONS: rFSH stimulation appears to influence oocyte quality and subsequent embryo quality in comparison with uFSH stimulation. FSH receptor polymorphisms seem to be an intrinsic factor influencing the ovarian response to FSH stimulation.


Assuntos
Hormônio Foliculoestimulante/farmacologia , Oócitos/crescimento & desenvolvimento , Injeções de Esperma Intracitoplásmicas , Técnicas de Cultura , Embrião de Mamíferos/fisiologia , Feminino , Hormônio Foliculoestimulante/urina , Humanos , Masculino , Folículo Ovariano/fisiologia , Polimorfismo Genético , Receptores do FSH/genética , Proteínas Recombinantes
19.
J Assist Reprod Genet ; 20(12): 506-12, 2003 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15035550

RESUMO

PURPOSE: To date, the impact of oocyte maturity at aspiration on the blastocyst formation in vitro has not been fully evaluated. This study was undertaken to assess the influence of oocyte maturity in patients undergoing in vitro fertilization and blastocyst transfer program. METHODS: A total of 1278 oocytes derived from 147-IVF cycles were retrospectivly analyzed. Oocyte maturity was graded on a scale from 1 to 5 based on the morphology of the ooplasm, cumulus mass, corona radiata, and membrana granulosa cells. RESULTS: Mature oocytes yielded the highest fertilization rates. Although the cleavage rates were similar in both groups, the percentage of poor morphology, day-3 embryos from the nonmature-oocyte group was significantly higher than from the mature-oocyte group (54.7% vs. 15.5%, P < 0.001). Although good morphology, day-3 embryos were collected from nonmature oocytes, the incidence of these embryos developing to the blastocyst stage was significantly less than from mature oocytes (33.3% vs. 71.2%, P < 0.001). Although blastocyst stage embryos were collected from nonmature oocytes, the incidence of these embryos developing to the top-scoring blastocysts was significantly less than from mature oocytes (58.3% vs. 89.5%, P < 0.001). CONCLUSIONS: These phenomena suggest that oocyte maturity produced in vivo determine the fertilization potential and subsequent blastocyst quality in vitro.


Assuntos
Blastocisto/fisiologia , Transferência Embrionária , Fertilização In Vitro , Oócitos/fisiologia , Feminino , Humanos , Técnicas In Vitro , Gravidez
20.
J Am Assoc Gynecol Laparosc ; 9(4): 468-73, 2002 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-12386358

RESUMO

STUDY OBJECTIVE: To compare intraoperative and postoperative complication rates for laparoscopic-assisted vaginal hysterectomy (LAVH) between women classified as obese, normal weight, or very thin based on body mass index (BMI). DESIGN: Retrospective cohort study (Canadian Task Force classification II-3). SETTING: University hospital. PATIENTS: Six hundred seventy women (162 with BMI >25, 34 with BMI <18.5, 474 with BMI 18.5-25 kg/m(2)). INTERVENTION: LAVH. MEASUREMENTS AND MAIN RESULTS: For women with high BMIs, 34 procedures (21.0%) were converted to laparotomy, compared with 48 (10.1%) for women of normal body weight and 3 (8.8%) for those with low BMI (p = 0.001). Average blood loss was 299.3 +/- 87.8, 219.1 +/- 57.5, and 231.8 +/- 65.9 ml, respectively (p <0.001). Very thin women had similar intraoperative and postoperative complication rates (8.8 %) as women of normal body weight (8.6%) and obese women (11.1%). CONCLUSION: Obese women had increased likelihood of conversion to laparotomy and greater blood loss after LAVH than nonobese women. Very thin women had similar intraoperative and postoperative complication rates as women of normal body weight and obese women.


Assuntos
Histerectomia Vaginal/métodos , Laparoscopia/métodos , Obesidade/complicações , Complicações Pós-Operatórias/etiologia , Doenças Uterinas/cirurgia , Adulto , Análise de Variância , Índice de Massa Corporal , Peso Corporal , Estudos de Coortes , Feminino , Seguimentos , Humanos , Histerectomia Vaginal/efeitos adversos , Histeroscopia/efeitos adversos , Histeroscopia/métodos , Incidência , Laparoscopia/efeitos adversos , Pessoa de Meia-Idade , Obesidade/diagnóstico , Complicações Pós-Operatórias/epidemiologia , Probabilidade , Valores de Referência , Estudos Retrospectivos , Medição de Risco , Resultado do Tratamento , Doenças Uterinas/patologia
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