Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 15 de 15
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Vet Microbiol ; 203: 136-142, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28619135

RESUMO

Gallibacterium anatis (G. anatis) has been suggested to have a causal role in salpingitis and peritonitis in egg-laying chickens, leading to decreased egg production and increased mortality worldwide. Adherence and invasion of epithelial cells are thought to play a role in the pathogenesis of G. anatis infection. The purpose of this article was to study adherence and invasion of G. anatis using two G. anatis strains of different virulence (Yu-PDS-RZ-1-SLG strain, highly virulent and F149T strain, non-virulent) via infection of the primary chicken oviduct epithelial cells (PCOECs).The results showed that Yu-PDS-RZ-1 -SLG strain was able to attach to PCOECs at higher levels than that of F149T strain, but no invasion was observed with either strain. However, cell debris and cell apoptosis were observed after being exposed to G. anatis Yu-PDS-RZ-1-SLG for 90min, whereas G. anatis F149T did not cause cell damage, and adherence was prevented by trypsin treatment of bacterial cells. Cytokines were detected by ELISA after infection, and the results showed that the expression of IL-6, TNF-α, and IFN-γ levels was higher in virulent strain infection than that of the avirulent group. Results also indicated that the highly virulent strain G. anatis displayed an increased level of adherence. Changes in cytokine profiles in this study suggested that the production of cytokines might influence the microenvironment of oviduct and promote adherence, serving as a possible mechanism inducing cell damage.


Assuntos
Galinhas/microbiologia , Infecções por Pasteurellaceae/veterinária , Pasteurellaceae/patogenicidade , Doenças das Aves Domésticas/microbiologia , Animais , Citocinas/metabolismo , Células Epiteliais/microbiologia , Feminino , Interações Hospedeiro-Patógeno , Oviductos/microbiologia , Óvulo/microbiologia , Infecções por Pasteurellaceae/microbiologia , Virulência
2.
Res Vet Sci ; 104: 83-5, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26850542

RESUMO

In recent years, there has been considerable interest in using adenoviruses as live vectors to develop recombinant vaccines. Previous studies have demonstrated the safety and effectiveness of HIV/SIV and influenza vaccine candidates based on human adenovirus type 4 (Ad4) replication-competent vectors in rhesus macaque and human model. To explore the possibility of human Ad4 vaccine strain used as a vector in developing porcine vaccines, the growth properties of replication-competent human Ad4 vaccine strain recombinant encoding EGFP in different porcine cell lines were investigated. All tested cell lines are permissive for Ad4 vaccine strain vector with varied replication efficiency. Thus, human Ad4 based vectors would be promising supplement to adenovirus vectors as a delivery vehicle for recombinant vaccines in swine industry.


Assuntos
Infecções por Adenoviridae/veterinária , Vacinas contra Adenovirus/imunologia , Adenovírus Humanos/crescimento & desenvolvimento , Adenovírus Humanos/imunologia , Doenças dos Suínos/imunologia , Infecções por Adenoviridae/imunologia , Infecções por Adenoviridae/virologia , Animais , Linhagem Celular , Suínos , Doenças dos Suínos/virologia , Vacinas Sintéticas/imunologia
3.
Protein Expr Purif ; 119: 51-6, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26616099

RESUMO

Previous research showed that a lectin from the mushroom Laetiporus sulphureus, designed LSL, bound to Sepharose and could be eluted by lactose. In this study, by taking advantage of the strong affinity of LSL-tag for Sepharose, we developed a single-step purification method for LSL-tagged fusion proteins. We utilized unmodified Sepharose-4B as a specific adsorbent and 0.2 M lactose solution as an elution buffer. Fusion proteins of LSL-tag and porcine circovirus capsid protein, designated LSL-Cap was recovered with purity of 90 ± 4%, and yield of 87 ± 3% from crude extract of recombinant Escherichia coli. To enable the remove of LSL-tag, tobacco etch virus (TEV) protease recognition sequence was placed downstream of LSL-tag in the expression vector, and LSL-tagged TEV protease, designated LSL-TEV, was also expressed in E. coli., and was recovered with purity of 82 ± 5%, and yield of 85 ± 2% from crude extract of recombinant E. coli. After digestion of LSL-tagged recombinant proteins with LSL-TEV, the LSL tag and LSL-TEV can be easily removed by passing the digested products through the Sepharose column. It is of worthy noting that the Sepharose can be reused after washing with PBS. The LSL affinity purification method enables rapid and inexpensive purification of LSL-tagged fusion proteins and scale-up production of native proteins.


Assuntos
Proteínas Recombinantes de Fusão/isolamento & purificação , Agaricales/química , Sequência de Aminoácidos , Sequência de Bases , Cromatografia de Afinidade/economia , Endopeptidases/química , Escherichia coli , Lectinas/química , Dados de Sequência Molecular , Proteólise , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/química , Sefarose/química
4.
Genome Announc ; 3(4)2015 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-26159524

RESUMO

A novel porcine reproductive and respiratory syndrome virus (PRRSV) strain with 393 nucleotide deletions in the nonstructural protein 2 (Nsp2) region was examined in this study. Results will help improve our understanding of the epidemiology and genetic diversity of the North American-type PRRSV in China.

5.
Bing Du Xue Bao ; 30(4): 353-8, 2014 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-25272586

RESUMO

This study aimed to understand the dynamic distribution of infectious bronchitis virus (IBV) Jin-13 strain in SPF chickens. Ninety-day-old SPF chickens were inoculated with Jin-13, a virulent strain, and dissected at day 1, 4, 7, 10, 14, 21, 28 or 35 post-inoculation (dpi). Samples of heart, liver, spleen, lung, trachea, kidney and duodenum were collected and the N gene was detected by Sybr Green I real-time quantitative RT-PCR assays. The established method had a good linear correlation from 7.77 x 10(8) to 10(0) copies/microL. SPF chickens developed typical clinical signs of IBV at the 4th dpi, and the IBV viral concentration of tissues and organs gradually increased with a peak of up to 7.13 x 10(4) copies/microL. The viral concentration of most organs decreased by the 10th dpi, but those of the kidney, trachea and lung remained positive for IBV at 28 dpi and the heart was still positive for IBV at > 35 dpi. The results of this study, showed that the Jin-13 strain can cause prolonged virus excertion in chickens with severe renal damage.


Assuntos
Infecções por Coronavirus/veterinária , Vírus da Bronquite Infecciosa/patogenicidade , Doenças das Aves Domésticas/virologia , Animais , Galinhas , Infecções por Coronavirus/virologia , Vírus da Bronquite Infecciosa/isolamento & purificação , Vírus da Bronquite Infecciosa/fisiologia , Pulmão/virologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Organismos Livres de Patógenos Específicos , Traqueia/virologia , Virulência
6.
Bing Du Xue Bao ; 30(4): 375-81, 2014 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-25272589

RESUMO

Encephalomyocarditis virus (EMCV) is a natural epidemic zoonotic pathogen. However, no reports have been published regarding the isolation, identification and full-length genome of EMCV from a local aardvark population. In present study, an EMCV isolate HNXX13 was isolated from aardvarks named Huainan-pig in Henan Province. The systematic identification, full-length genome sequencing and molecular characteristic analysis of the isolate HNXX13 were conducted. The result showed that the isolate was spherical with a diameter of 24-30 nm, neither heat- nor acid-resistant, sensitive to trypsin, insensitive to chloroform, not protected by bivalent cationic, and the specific fluorescence was observed in the cytoplasm of BHK-21 cells infected with the isolate by using indirect fluorescence assay. The full-length genome of EMCV HNXX13 generated a 7 725bp sequence (GenBank: F771002), with 81.0%-99.9% nucleotide identity to reference strains from different animals, and 99.5% with a Chinese reference strain isolated earlier from a commercial pig herd. The phylogenetic tree based on the full-length genome and ORF sequences identified that all EMCV strains were divided into three groups G1, G2 and G3, and strain HNXX13 belonging to the G1 group with other Chinese reference strains. The result also identified that this EMCV infection could cause severe clinical signs in a local aardvark population, and enriches the molecular epidemiological data of EMCV in China. Regional differences exist in EMCV genome and transmission is limited within a certain area. However, the cross-infection and transmission of EMCV between aardvark and mice appears most likely. Mutations have occurred in some amino acids of EMCV strain HNXX13 during the transmission in local aardvark herd and these mutations might make the virus easier to infect the aardvark.


Assuntos
Infecções por Cardiovirus/veterinária , Vírus da Encefalomiocardite/isolamento & purificação , Genoma Viral , Animais , Animais Selvagens/virologia , Infecções por Cardiovirus/virologia , China , Vírus da Encefalomiocardite/classificação , Vírus da Encefalomiocardite/genética , Camundongos , Dados de Sequência Molecular , Filogenia
7.
Bing Du Xue Bao ; 30(4): 441-9, 2014 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-25272601

RESUMO

In early 2011, the serious outbreak of porcine pseudorabies virus (PRV) infection suddenly recurred in Henan and neighboring Provinces. To investigate the etiology of massive infection with PRV, 16 800 serum samples, 905 porcine epidemic diarrhea virus (PEDV) back-feeding tissues, and 56 PR gene deleted live vaccines were colleted from January 2011 to May 2013 to detect PRV field infection using a PRV gE antibody test kit. The gE and TK genes of 11 new epidemic PRV strains were sequenced by PCR, and their molecular characteristics were analyzed. Moreover, virus titer determination, protective test against PRV, and vaccine potency testing were performed. The results showed that the detection rate of PRV field infection-positive pig farms was 68.06%, and the overall positive rate of PRV field infection in serum was 38.47%; the positive rates in breeding sows, breeding boars, reserve pigs, and commercial pigs were 40.12%, 30.88%, 54.67%, and 26.52%, respectively. The new epidemic strains were in the same evolutionary branch and belonged to the virulent strain group. Compared with the classical PRV strain, the virulence of new epidemic strains changed a little. The length of gE gene was 1 787 bp, and the length of TK gene was 963 bp. The nucleotide homologies of gE and TK genes to Chinese reference strains were 98.2%-99.8% and 98.90%-99.6%, respectively, and the amino acid homologies were 97.1%-99.8% and 97.5%-99.4%, respectively. Commercial vaccine had a 100% protective effect against the new epidemic strains. The positive rate of PRV field infection was 0% in vaccine and 40.44% in back-feeding tissues. The results confirmed that PRV field infection rates were rising sharply among pigs in Henan and neighboring Provinces after 2011. The main virulence genes of new epidemic PRV strains did not change significantly over the years. PR gene deleted live vaccines had no PRV field infection and could completely resist the attack of new strains. The virus carriage of breeding boars and reserve pigs and the serious PRV field infection in PEDV back-feeding tissues were the main causative factors for massive infection with PRV and epidemic outbreak in Henan and neighboring Provinces from 2011 to 2013.


Assuntos
Herpesvirus Suídeo 1/isolamento & purificação , Pseudorraiva/epidemiologia , Pseudorraiva/virologia , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/virologia , Sequência de Aminoácidos , Ração Animal/análise , Ração Animal/virologia , Animais , China/epidemiologia , Epidemias , Feminino , Herpesvirus Suídeo 1/química , Herpesvirus Suídeo 1/classificação , Herpesvirus Suídeo 1/genética , Masculino , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Sus scrofa , Suínos , Proteínas Virais/química , Proteínas Virais/genética
8.
PLoS One ; 9(6): e100264, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24949637

RESUMO

Shigellosis in chickens was first reported in 2004. This study aimed to determine the pathogenicity of Shigella in chickens and the possibility of cross-infection between humans and chickens. The pathogenicity of Shigella in chickens was examined via infection of three-day-old SPF chickens with Shigella strain ZD02 isolated from a human patient. The virulence and invasiveness were examined by infection of the chicken intestines and primary chicken intestinal epithelial cells. The results showed Shigella can cause death via intraperitoneal injection in SPF chickens, but only induce depression via crop injection. Immunohistochemistry and transmission electron microscopy revealed the Shigella can invade the intestinal epithelia. Immunohistochemistry of the primary chicken intestinal epithelial cells infected with Shigella showed the bacteria were internalized into the epithelial cells. Electron microscopy also confirmed that Shigella invaded primary chicken intestinal epithelia and was encapsulated by phagosome-like membranes. Our data demonstrate that Shigella can invade primary chicken intestinal epithelial cells in vitro and chicken intestinal mucosa in vivo, resulting in pathogenicity and even death. The findings suggest Shigella isolated from human or chicken share similar pathogenicity as well as the possibility of human-poultry cross-infection, which is of public health significance.


Assuntos
Galinhas/microbiologia , Shigella/patogenicidade , Animais , Células HeLa , Humanos , Mucosa Intestinal/microbiologia , Shigella/isolamento & purificação , Organismos Livres de Patógenos Específicos , Virulência
9.
J Vet Sci ; 15(3): 399-407, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24675838

RESUMO

A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG ODN than animals immunized with rAd/Cap/518 alone. The frequencies of IL-2-secreting CD4⁺ T cells and IFN-γ-producing CD8⁺ T cells were significantly higher in the combined immunization group than mice immunized with rAd/Cap/518 alone. The frequencies of CD3⁺, CD3⁺CD4⁺CD8⁻, and CD3⁺CD4⁻CD8⁺ T cells in the combined immunization group were similar to that treated with CpG ODN alone, but significantly higher than mice that did not receive CpG ODN. PCV-2 load after challenge in the combined immunization group was significantly lower than that in the phosphate-buffered saline placebo group and approximately 7-fold lower in the group treated with CpG ODN alone. These results indicate that rAd/Cap/518 combined with CpG ODN can enhance systemic and local mucosal immunity in mice, and represent a promising synergetic mucosal vaccine against PCV-2.


Assuntos
Proteínas do Capsídeo/genética , Infecções por Circoviridae/imunologia , Circovirus/genética , Vacinas Virais/genética , Adenoviridae/genética , Adenoviridae/imunologia , Administração Intranasal , Animais , Proteínas do Capsídeo/imunologia , Circovirus/imunologia , Epitopos/genética , Epitopos/imunologia , Feminino , Imunidade nas Mucosas/imunologia , Imunoglobulina A/sangue , Imunoglobulina A/imunologia , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Oligodesoxirribonucleotídeos/genética , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologia , Vacinas Virais/administração & dosagem , Vacinas Virais/imunologia
10.
Arch Virol ; 158(12): 2487-94, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23797760

RESUMO

Acute diarrhea outbreaks caused by porcine epidemic diarrhea virus (PEDV) have been observed in various pig-breeding provinces of China since December 2010. Endemic strains of PEDV were isolated from different areas, and the complete genome sequences of 10 isolates were determined. Our objective in this study was to genetically characterize current Chinese field isolates of PEDV to better understand their epidemiology and genetic diversity. Sequence analysis showed that 10 post-2010 isolates shared high homology with each other and were always clustered together with the virulent DR13 strains (South Korea) and/or one earlier Chinese strain, CH-S, in phylogenetic analysis. All post-2010 isolates possessed common sequence changes in each gene. Our results suggest that current Chinese PEDV isolates originated from either South Korean and/or Chinese ancestors that underwent some genetic variation, thereby forming a new PEDV genotype in China.


Assuntos
Infecções por Coronavirus/veterinária , Diarreia/veterinária , Doenças Endêmicas , Vírus da Diarreia Epidêmica Suína/classificação , Vírus da Diarreia Epidêmica Suína/genética , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/virologia , Animais , China/epidemiologia , Análise por Conglomerados , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Diarreia/epidemiologia , Diarreia/virologia , Variação Genética , Genoma Viral , Epidemiologia Molecular , Filogenia , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , RNA Viral/genética , Análise de Sequência de DNA , Homologia de Sequência , Suínos
11.
Bing Du Xue Bao ; 29(2): 197-205, 2013 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-23757853

RESUMO

Since late 2010, porcine epidemic diarrhea virus (PEDV) has been re-emerging in central China. To explore the possible reason of the PEDV outbreaks, twelve PEDV field strains were isolated from different swine breeding farms in central China during 2010-2012, and molecular diversity, phylogenetic relationships of these strains with other PEDV reference strains were investigated. Sequence analysis of S, M and ORE3 genes revealed that the central China PEDV isolates had several specific nucleotides and amino acids which were different from PEDV reference strains. In addition, the entire S genes of eleven central China PEDV isolates were found to be nine nucleotides longer in length than CV777 and large number of amino acid variations was accumulated in the N-terminal region of S gene. Phylogenetic analysis showed that the central China PEDV isolates had close relationship with Korea strains (2007-2009), Thailand strains (2007-2008), Vietnam strains (2009-2010), Japan strains (2010), and other prevailing strains from other parts of China (2010-2012). However, they differed genetically from European strains (CV777, Brl/87), China strains (2003-2007) and the vaccine strains (CV777) used in China. These results imply that a rapid variation and evolution of central China PEDV strains has occurred in recent years, and a more efficient vaccine strain should be selected to prevent and control outbreaks of PEDV in China.


Assuntos
Filogenia , Vírus da Diarreia Epidêmica Suína/classificação , Vírus da Diarreia Epidêmica Suína/genética , Doenças dos Suínos/virologia , Animais , China/epidemiologia , Surtos de Doenças , Fezes/virologia , Dados de Sequência Molecular , Fases de Leitura Aberta , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Suínos , Doenças dos Suínos/epidemiologia , Proteínas Virais/genética
12.
Vet Immunol Immunopathol ; 154(1-2): 48-53, 2013 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-23618367

RESUMO

Porcine circovirus-2 (PCV-2) is primarily transmitted through mucosa, thus the mucosal immunity may constitute an essential feature of vaccination strategies against PCV-2 infection. Mucosal immunity elicited by recombinant replication-deficient adenovirus expressing the major epitopes of PCV-2 capsid protein (rAd/Cap/518) via intranasal (i.n.), intramuscular (i.m.) or oral routes in mice were evaluated. Immunization with rAd/Cap/518 via i.n. route induced higher titers of IgA in saliva, bronchoalveolar and intestinal lavage fluid compared with those immunized via i.m. route. The proportions of CD3+, CD3+CD4+ and CD3+CD8+ T cells were significantly increased in mice immunized with rAd/Cap/518 via i.n. route compared with the control group. Higher levels of IFN-γ were detected in the spleen and mesenteric lymph nodes of mice immunized with rAd/Cap/518 via i.n. route compared with other groups, yet IL-4 was not detected in any group. Real-time PCR analysis confirmed viral DNA loads in the i.m. or i.n. immunization group was lower than that seen in the rAd immunization. These results indicate that i.n. administration of rAd/Cap/518 can elicit humoral and Th1-type cellular protective immunity in both systemic and mucosal immune compartments in mice, representing a promising mucosal vaccine candidate against PCV-2.


Assuntos
Proteínas do Capsídeo/imunologia , Infecções por Circoviridae/veterinária , Circovirus/imunologia , Epitopos/metabolismo , Imunidade nas Mucosas/imunologia , Vacinas Virais/imunologia , Adenoviridae , Animais , Anticorpos Antivirais/sangue , Infecções por Circoviridae/prevenção & controle , DNA Viral , Imunoglobulina G/sangue , Camundongos , Camundongos Endogâmicos BALB C , Distribuição Aleatória , Carga Viral
13.
Genome Announc ; 1(1)2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23469356

RESUMO

We report here the complete genome sequence of the porcine epidemic diarrhea virus strain CH/ZMDZY/11 isolated from central China. Our data, together with sequence data of porcine epidemic diarrhea virus (PEDV) isolates from other parts in China, will help to understand better the epidemiology and genetic diversity of PEDV field isolates in China.

14.
Virus Genes ; 46(2): 337-44, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23269482

RESUMO

Porcine epidemic diarrhea has re-emerged with devastating impact in central China since October 2010. To investigate and analyze the reason of this outbreak, the M and ORF3 genes of 15 porcine epidemic diarrhea viruses (PEDV), which were collected from different areas of central China during October 2010 and December 2011, were amplified by reverse transcriptase polymerase chain reaction, cloned, sequenced, and analyzed. Sequence analyses showed that the nucleotides and amino acids were changed at some sites in the M and ORF3 genes of the 15 PEDV strains compared with those genes of CV777 reference strain. Based on the phylogenetic analyses, PEDVs in central China and reference strains could be separated into three groups: G1, G2, and G3. The 15 PEDV strains belonged to G3 group and showed a close relationship with Korean strains (2007), Thai strains (2007-2008), and partial other Chinese strains (2010-2011), but differed genetically from European strains (Br1/87) and the vaccine strain (CV777 vs) being used in China. Furthermore, all 15 PEDV strains from central China and some other isolates in China from 2003 to 2007 (LJB-03, QH, and LZC) belonged to different group. Therefore, PEDV exhibits rapid variation and genetic evolution, and the currently prevailing PEDV strains in central China are a new genotype.


Assuntos
Diarreia/veterinária , Variação Genética , Vírus da Diarreia Epidêmica Suína/genética , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Doenças dos Suínos/virologia , Sequência de Aminoácidos , Animais , Sequência de Bases , China/epidemiologia , Diarreia/epidemiologia , Diarreia/virologia , Fezes/virologia , Dados de Sequência Molecular , Proteínas Associadas à Matriz Nuclear/genética , Fases de Leitura Aberta , Filogenia , Vírus da Diarreia Epidêmica Suína/classificação , Suínos , Doenças dos Suínos/epidemiologia
15.
Bing Du Xue Bao ; 28(4): 424-30, 2012 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-22978169

RESUMO

To meet the needs of detection of infectious bursal disease virus (IBDV) under high efficient culture, a SYBR Green I real-time RT-PCR (qRT-PCR) was developed using a pair of primers specific to the conserved region of VP4 gene of IBDV and compared with TCID50 method by monitoring the proliferation dynamics of IBDV in DF-1 cell line adherent to micro carrier in tubular reactor. The results showed that the RT-PCRassay was linear in the range of 4. 03 X 10(1)-10(9) copies/microL. The IBDV RNA detection limit was 40 copies/microL, which was 1 000 times more sensitive than conventional PCR. No cross-reactions with other viruses was observed. The intra-assay coefficient of variation was less than 0.05%. There was a parallel correlation of IBDV proliferation dynamics in DF-1 cell under Micro carrier suspension and static adherent culture by the qRT-PCR assay and TCID50 method. The detection results of the IBDV samples from tubular and flask culture showed the differences of the micro carrier and adherent culture by both methods. In conclusion, the qRT-PCR assay is more rapid and sensitive than the TCID50 method, which is more appropriate for the real time detection of IBDV.


Assuntos
Vírus da Doença Infecciosa da Bursa/genética , Vírus da Doença Infecciosa da Bursa/isolamento & purificação , Compostos Orgânicos/metabolismo , Reação em Cadeia da Polimerase em Tempo Real/métodos , Animais , Calibragem , Linhagem Celular , Sequência Conservada , Primers do DNA/genética , Compostos Orgânicos/química , Reprodutibilidade dos Testes , Espectrometria de Fluorescência , Proteínas Virais/genética , Replicação Viral
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA