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1.
Int J Biol Sci ; 16(6): 970-980, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32140066

RESUMO

Osteoarthritis (OA) is a whole-joint disease characterized by cartilage destruction, subchondral bone sclerosis, osteophyte formation, and synovitis. However, it remains unclear which part of the joint undergoes initial pathological changes that drives OA onset and progression. In the present study, we investigated the longitudinal alterations of the entire knee joint using a surgically-induced OA mouse model. Histology analysis showed that synovitis occurred as early as 1 week after destabilization of the medial meniscus (DMM), which preceded the events of cartilage degradation, subchondral sclerosis and osteophyte formation. Importantly, key pro-inflammatory cytokines such as IL-1ß, IL-6, TNFα, and Ccl2, major matrix degrading enzymes including Adamts4, Mmp3 and Mmp13, as well as nerve growth factor (NGF), all increased significantly in both synovium and articular cartilage. It is notable that the inductions of these factors in synovium are far more extensive than those in articular cartilage. Results from behavioral tests demonstrated that sensitization of knee joint pain developed after 8 weeks, later than histological and molecular changes. In addition, the nanoindentation modulus of the medial tibiae decreased 4 weeks after DMM surgery, simultaneous with histological OA signs, which is also later than appearance of synovitis. Collectively, our data suggested that synovitis precedes and is associated with OA, and thus synovium may be an important target to intervene in OA treatment.

2.
J Pediatr Surg ; 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31575410

RESUMO

BACKGROUND: To investigate the safety, feasibility, and complications of pancreatectomies for pediatric pancreatic tumors. METHODS: The medical records of pancreatectomy patients from January 2007 to January 2018 were retrospectively analyzed for perioperative factors and complications. Patients were divided into pancreatic head (n = 43), body (n = 18) and tail (n = 43) groups. RESULTS: Seventy-two girls and 32 boys (median age 10 years at diagnosis, range: 0-15 years) were enrolled and had solid pseudopapillary tumors (n = 73), pancreatoblastoma (n = 19), neuroendocrine tumors (n = 9), and others. Primary surgical procedures included pylorus-preserving pancreaticoduodenectomy (n = 10) and distal pancreatectomy with splenectomy (n = 4), and organ-sparing resection procedures included duodenum-preserving pancreas head resection (n = 25), middle segmental pancreatic resection (n = 15), spleen-preserving distal pancreatectomy (n = 37) and local enucleation (n = 13), with a median blood loss of 20 cm3 (range: 10-300 cm3). Short-term complications included pancreatic fistula (35.6%), bile leakage (2.9%), intraabdominal infection (21.2%), delayed gastric emptying (23.1%), and postpancreatectomy hemorrhage (5.8%). After a median follow-up of 38 months (range: 3-143 months), 94 patients (90.4%) were alive without tumor recurrence, 2 were alive after tumor recurrence, 1 pancreatoblastoma patient died from tumor recurrence, and 7 were lost to follow-up. Only 14 patients (14/96, 14.6%) had long-term complications at the outpatient follow-up. CONCLUSIONS: Surgical resection was the main treatment for pancreatic tumors. Organ-sparing resection procedures led to good long-term results for pediatric pancreatic tumors, even if these procedures could cause a relatively high incidence of short-term complications (especially pancreatic fistula and postpancreatectomy hemorrhage). LEVEL OF EVIDENCE: Level IV.

3.
HPB (Oxford) ; 2019 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-31409540

RESUMO

BACKGROUND: To investigate the safety, feasibility, and complications of using duodenum-preserving pancreas head resection (DPPHR) to treat pediatric benign and low-grade malignant pancreatic head tumors. METHODS: Patients with pancreatic head tumors that underwent resection were retrospectively analyzed for perioperative factors and postoperative complications. RESULTS: Thirty-five patients with a median age of 10 years at diagnosis were identified. Patients were divided by procedures into the DPPHR (n = 22), local enucleation (n = 7) and pylorus-preserving pancreatoduodenectomy (PPPD, n = 6) groups. No significant difference was found in operation time between the DPPHR and PPPD groups (P > 0.05). Significantly, longer drainage time, duration of somatostatin use and hospital stay were observed in the DPPHR group than in the PPPD group (P < 0.05). The incidences of short-term complications were not significantly different among the three groups (P > 0.05). The incidence of long-term complications was markedly lower in both the DPPHR and local enucleation groups than in the PPPD group (P < 0.05). CONCLUSION: DPPHR might be a safe treatment option for pediatric patients with benign and low-grade malignant pancreatic head tumors. The incidence of long-term complications was significantly lower with DPPHR. However, perioperative management might be challenging for surgeons.

4.
Cells ; 8(6)2019 06 04.
Artigo em Inglês | MEDLINE | ID: mdl-31167434

RESUMO

Periodontitis is a prevalent infectious disease worldwide, causing the damage of periodontal support tissues, which can eventually lead to tooth loss. The goal of periodontal treatment is to control the infections and reconstruct the structure and function of periodontal tissues including cementum, periodontal ligament (PDL) fibers, and bone. The regeneration of these three types of tissues, including the re-formation of the oriented PDL fibers to be attached firmly to the new cementum and alveolar bone, remains a major challenge. This article represents the first systematic review on the cutting-edge researches on the regeneration of all three types of periodontal tissues and the simultaneous regeneration of the entire bone-PDL-cementum complex, via stem cells, bio-printing, gene therapy, and layered bio-mimetic technologies. This article primarily includes bone regeneration; PDL regeneration; cementum regeneration; endogenous cell-homing and host-mobilized stem cells; 3D bio-printing and generation of the oriented PDL fibers; gene therapy-based approaches for periodontal regeneration; regenerating the bone-PDL-cementum complex via layered materials and cells. These novel developments in stem cell technology and bioactive and bio-mimetic scaffolds are highly promising to substantially enhance the periodontal regeneration including both hard and soft tissues, with applicability to other therapies in the oral and maxillofacial region.


Assuntos
Cemento Dentário/fisiologia , Ligamento Periodontal/fisiologia , Regeneração/fisiologia , Células-Tronco/metabolismo , Terapia Genética , Humanos , Periodontite/patologia , Periodontite/terapia , Transplante de Células-Tronco , Células-Tronco/citologia , Engenharia Tecidual , Tecidos Suporte/química
5.
Dent Mater ; 35(8): 1104-1116, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31104922

RESUMO

OBJECTIVE: Secondary caries and micro-cracks are the main limiting factors for dentin bond durability. The objectives of this study were to develop a self-healing adhesive containing dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate the effects of water-aging for 12 months on self-healing, dentin bonding, and antibacterial properties for the first time. METHODS: Microcapsules were synthesized with poly (urea-formaldehyde) (PUF) shells containing triethylene glycol dimethacrylate (TEGDMA) and N,N-dihydroxyethyl-p-toluidine (DHEPT). The adhesive contained 7.5% microcapsules, 10% DMAHDM, and 20% NACP (all mass). Specimens were water-aged at 37 °C for 1 day to 12 months. Dentin bond strength was measured using extracted human teeth. A single-edge-V-notched-beam (SEVNB) method was used to measure fracture toughness KIC and self-healing efficiency. A dental plaque microcosm biofilm model was used with human saliva as inoculum. RESULTS: The microcapsules + DMAHDM + NACP group showed no decline in dentin bond strength after water-aging for 12 months, which was significantly higher than that of other groups without DMAHDM (p < 0.05). A self-healing efficiency of 67% recovery in KIC was obtained even after 12 months of water immersion, indicating that the self-healing ability was not lost in water-aging (p > 0.1). The bacteria-killing ability of this adhesive did not decline from 1 day to 12 months (p > 0.1), with biofilm CFU reduction by 3-4 orders of magnitude after the resin was water-aged for 12 months, compared to control resin. SIGNIFICANCE: This novel adhesive with triple merits of self-healing, antibacterial and remineralization functions showed an excellent long-term durability in water-aging for 12 months. This multifunctional adhesive has the potential for dental applications to heal cracks, inhibit bacteria, provide ions for remineralization, and increase the restoration longevity.


Assuntos
Cimentos Dentários , Água , Antibacterianos , Biofilmes , Pré-Escolar , Humanos , Lactente , Metacrilatos
6.
Dent Mater ; 35(7): 1031-1041, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31076156

RESUMO

OBJECTIVE: Calcium phosphate cements (CPCs) mimic nanostructured bone minerals and are promising for dental, craniofacial and orthopedic applications. Vascularization plays a critical role in bone regeneration. This article represents the first review on cutting-edge research on prevascularization of CPC scaffolds to enhance bone regeneration. METHODS: This article first presented the prevascularization of CPC scaffolds. Then the co-culture of two cell types in CPC scaffolds was discussed. Subsequently, to further enhance the prevascularization efficacy, tri-culture of three different cell types in CPC scaffolds was presented. RESULTS: (1) Arg-Gly-Asp (RGD) incorporation in CPC bone cement scaffold greatly enhanced cell affinity and bone prevascularization; (2) By introducing endothelial cells into the culture of osteogenic cells (co-culture of two different cell types, or bi-culture) in CPC scaffold, the bone defect area underwent much better angiogenic and osteogenic processes when compared to mono-culture; (3) Tri-culture with an additional cell type of perivascular cells (such as pericytes) resulted in a substantially enhanced prevascularization of CPC scaffolds in vitro and more new bone and blood vessels in vivo, compared to bi-culture. Furthermore, biological cell crosstalk and capillary-like structure formation made critical contributions to the bi-culture system. In addition, the pericytes in the tri-culture system substantially promoted stability and maturation of the primary vascular network. SIGNIFICANCE: The novel approach of CPC scaffolds with stem cell bi-culture and tri-culture is of great significance in the regeneration of dental, craniofacial and orthopedic defects in clinical practice.


Assuntos
Cimentos para Ossos , Engenharia Tecidual , Fosfatos de Cálcio , Diferenciação Celular , Técnicas de Cocultura , Osteogênese , Células-Tronco , Tecidos Suporte
7.
Cancer Treat Rev ; 74: 49-60, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30831375

RESUMO

Immune checkpoint inhibitors-based immunotherapy offers a new effective modality in the treatment of advanced malignancies. Considering the remarkable efficacy of immune checkpoint inhibitors in clinical trials, the FDA has approved a variety of immune checkpoint inhibitors for the treatment of advanced tumors. However, only limited patients with certain cancers can benefit from monotherapy of immune checkpoint inhibitors. Interventional therapy for cancer can not only destroy the primary tumors, but also regulate the immune system through different mechanisms, which provides a potential possibility for the combination of immune checkpoint inhibitors and interventional modalities in cancer treatment. This article reviews the possible synergistic mechanisms of interventional therapy combined with immune checkpoint inhibitors and summarizes the research progress of the combined therapy in cancer treatment.


Assuntos
Antineoplásicos Imunológicos/uso terapêutico , Antígeno B7-H1/antagonistas & inibidores , Antígeno CTLA-4/antagonistas & inibidores , Neoplasias/terapia , Proteína 2 Ligante de Morte Celular Programada 1/antagonistas & inibidores , Receptor de Morte Celular Programada 1/antagonistas & inibidores , Técnicas de Ablação , Animais , Antígeno B7-H1/imunologia , Braquiterapia , Antígeno CTLA-4/imunologia , Quimioembolização Terapêutica , Humanos , Imunoterapia/métodos , Neoplasias/tratamento farmacológico , Neoplasias/imunologia , Proteína 2 Ligante de Morte Celular Programada 1/imunologia , Receptor de Morte Celular Programada 1/imunologia
8.
Pediatr Res ; 85(6): 885-894, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30718793

RESUMO

BACKGROUND: Opsoclonus-myoclonus syndrome (OMS) is a rare neurological disorder, usually accompanied by neuroblastoma (NB). There is no targeted treatment and animal model of OMS. We aimed to investigate whether insulin-like growth factor 1 (IGF-1)/phosphoinositide 3-kinase (PI3K) signaling alleviates neuronal cytolysis in pediatric OMS. METHODS: Cultured rat cerebral cortical neurons and cerebellar neurons were incubated with sera or IgG isolated from sera of children with OMS and NB. Cytolysis and PI3K expression were measured by the lactate dehydrogenase assay and enzyme-linked immunosorbent assay, respectively. Using inhibitors and activators, the effects of IGF-1 and PI3K on cytolysis were investigated. RESULTS: The incubation of sera or IgG from children with OMS and NB increased cytolysis in not only cerebellar neurons, but also cerebral cortical neurons. Furthermore, the IGF-1 receptor antagonist NVP-AEW541 exaggerated cytolysis in children with OMS and NB. IGF-1 alleviated cytolysis, which was blocked by the PI3K inhibitor LY294002. Additionally, sera or IgG from children with OMS and NB compensatively elevated PI3K expression. LY294002 exacerbated cytolysis; whereas, the PI3K activator 740 Y-P suppressed cytolysis. CONCLUSION: IGF-1/PI3K signaling alleviates the cytolysis of cultured neurons induced by serum IgG from children with OMS and NB, which may be innovation therapy targets.

9.
J Dent ; 81: 91-101, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30599165

RESUMO

OBJECTIVES: The dentin bonding often fails over time, leading to secondary caries and restoration failure. The objectives of this study were to develop an adhesive with dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate the effects of storage in artificial saliva for six months on the bonding durability, antibacterial activity, ion release and biofilm pH properties for the first time. METHODS: DMAHDM was added at 5% (by mass) to Scotchbond Primer and Adhesive (SBMP). NACP was added at 10%, 20%, and 30% to SBMP adhesive. Dentin bonding durability, antibacterial activity against Streptococcus mutans biofilms, and calcium (Ca) and phosphate (P) ion liberation properties were investigated after 1 day and 6months of storage in artificial saliva. RESULTS: Dentin bond strength (n = 50) had 25% loss after 6 months of aging for SBMP control. However, SBMP + DMAHDM+10NACP and SBMP + DMAHDM+20NACP showed no loss in bond strength after storage in artificial saliva for 6 months. The DMAHDM + NACP incorporation method dramatically reduced the biofilm metabolic activity and acid production, and decreased the biofilm CFU by four orders of magnitude, compared to SBMP control, even after 6 months of aging (p < 0.05). DMAHDM + NACP had long-lasting Ca and P ion releases, and raised the biofilm pH to 6.8, while the control group had a cariogenic biofilm pH of 4.5. CONCLUSIONS: Incorporating DMAHDM + NACP in bonding agent yielded potent and long-lasting antibacterial activity and ions liberation ability, and much higher long-term dentin bond strength after 6-month of aging. The new bonding agent is promising to inhibit caries at the restoration margins and increase the resin-dentin bonding longevity. CLINICAL SIGNIFICANCE: The novel bioactive adhesive is promising to protect tooth structures from biofilm acids and secondary caries. NACP and DMAHDM have great potential for applications to a wide range of dental materials to reduce plaque and achieve therapeutic effects.


Assuntos
Antibacterianos , Fosfatos de Cálcio , Cimentos Dentários , Nanopartículas , Biofilmes , Concentração de Íons de Hidrogênio , Teste de Materiais , Metacrilatos
10.
Implant Dent ; 27(5): 555-563, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30134266

RESUMO

PURPOSE: To investigate the effect(s) of screw-access hole (SAH) in different diameters on the cement-retained implant prosthodontic systems and surrounding cortical bones. MATERIALS AND METHODS: Twenty finite element models were divided into 4 groups: 2 types of full-contour (FC) crowns (Y-TZP, gold alloy) and 2 types of porcelain-fused-to-metal crowns (based on Co-Cr, Au-Pd alloy). For each group, 5 crowns were simulated by varying the diameter of SAH (0, 1, 2, 3, and 4 mm). A vertical load of 200 N and an oblique load of 100 N (45°s) were applied. All models were analyzed with finite element analysis software. RESULTS: The stress on the occlusal surface of crowns was almost unchanged when the SAH was within 0 to 3 mm, whereas it showed an obvious increase when it reached 4 mm. The stress concentration was also suddenly changed from the loading area to the hole margin under vertical loading. As for the screw, a lower stress level was observed in vertical loading when an FC crown with an SAH within 0 to 1 mm was applied. The stress concentration was constantly located at the beginning of the first thread. Stresses of other components remained almost unchanged. CONCLUSIONS: From the aspect of biomechanics, an FC crown with a 1-mm access hole is recommended when a combined cement- and screw-retained crown was used in the posterior region.


Assuntos
Coroas , Cimentos Dentários/uso terapêutico , Projeto do Implante Dentário-Pivô , Implantes Dentários , Prótese Dentária Fixada por Implante , Adulto , Fenômenos Biomecânicos , Osso Cortical/patologia , Análise do Estresse Dentário , Análise de Elementos Finitos , Humanos , Masculino , Mandíbula/patologia
11.
Dent Mater ; 34(9): 1310-1322, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29935766

RESUMO

OBJECTIVES: A nanoparticle-doped adhesive that can be controlled with magnetic forces was recently developed to deliver drugs to the pulp and improve adhesive penetration into dentin. However, it did not have bactericidal and remineralization abilities. The objectives of this study were to: (1) develop a magnetic nanoparticle-containing adhesive with dimethylaminohexadecyl methacrylate (DMAHDM), amorphous calcium phosphate nanoparticles (NACP) and magnetic nanoparticles (MNP); and (2) investigate the effects on dentin bond strength, calcium (Ca) and phosphate (P) ion release and anti-biofilm properties. METHODS: MNP, DMAHDM and NACP were mixed into Scotchbond SBMP at 2%, 5% and 20% by mass, respectively. Two types of magnetic nanoparticles were used: acrylate-functionalized iron nanoparticles (AINPs); and iron oxide nanoparticles (IONPs). Each type was added into the resin at 1% by mass. Dentin bonding was performed with a magnetic force application for 3min, provided by a commercial cube-shaped magnet. Dentin shear bond strengths were measured. Streptococcus mutans biofilms were grown on resins, and metabolic activity, lactic acid and colony-forming units (CFU) were determined. Ca and P ion concentrations in, and pH of biofilm culture medium were measured. RESULTS: Magnetic nanoparticle-containing adhesive using magnetic force increased the dentin shear bond strength by 59% over SBMP Control (p<0.05). Adding DMAHDM and NACP did not adversely affect the dentin bond strength (p>0.05). The adhesive with MNP+DMAHDM+NACP reduced the S. mutans biofilm CFU by 4 logs. For the adhesive with NACP, the biofilm medium became a Ca and P ion reservoir. The biofilm culture medium of the magnetic nanoparticle-containing adhesive with NACP had a safe pH of 6.9, while the biofilm medium of commercial adhesive had a cariogenic pH of 4.5. SIGNIFICANCE: Magnetic nanoparticle-containing adhesive with DMAHDM and NACP under a magnetic force yielded much greater dentin bond strength than commercial control. The novel adhesive reduced biofilm CFU by 4 logs and increased the biofilm pH from a cariogenic pH 4.5-6.9, and therefore is promising to enhance the resin-tooth bond, strengthen tooth structures, and suppress secondary caries at the restoration margins.


Assuntos
Antibacterianos/síntese química , Antibacterianos/farmacologia , Fosfatos de Cálcio/farmacologia , Adesivos Dentinários/síntese química , Adesivos Dentinários/farmacologia , Nanopartículas de Magnetita/química , Metacrilatos/farmacologia , Remineralização Dentária/métodos , Biofilmes/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Cimentos de Resina/farmacologia , Células-Tronco , Streptococcus mutans/efeitos dos fármacos
12.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 36(2): 212-214, 2018 Apr 01.
Artigo em Chinês | MEDLINE | ID: mdl-29779286

RESUMO

Implant restoration has become one of the most regular methods of restoring dentition defect or edentulous. Implant placement and osseointegration are partly unreserved (fracture, implant is not in the correct three-dimensional position and cannot be repaired, peri-implantitis-affected nonmobile implants) need to be removed. This article reviews the different methods of removing implants and discusses the limitations of each method, as well as the complications that may occur during the procedure.


Assuntos
Implantes Dentários , Falha de Restauração Dentária , Peri-Implantite , Seguimentos , Humanos , Osseointegração , Resultado do Tratamento
13.
Sci Total Environ ; 621: 1310-1319, 2018 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-29054640

RESUMO

Understanding the dynamics of organic matter (OM) at global and local scales is one of the challenges in the environmental sciences and i.e. terrestrial biogeochemistry. The accurate identification of OM is an essential element to achieve this goal. In our study, a novel application for quantitatively apportioning sources of eroded sedimentary OM from an eco-geomorphologic perspective was shown successfully via a coupled molecular n-alkane biomarkers and stable isotopic signatures (13C and 15N) along with elemental compositions (TOC and TN) using a Bayesian mixing model (SIAR). Soil source samples were collected from different land use types (i.e., forests, grassland, cropland, and fallow) and gully, which were probably transported downstream along the steep terrain. Meanwhile, three soil profiles with a total of 90 sediment samples were also sampled in check dam. The results indicated that cropland was the main sedimentary OM source in this catchment, contributing 29.5%, whereas the forests, grassland, fallow and gully contributed 12.17%, 15.39%, 21.53% and 21.85%, respectively. Although the molecular biomarker as a tracer was not valid solely, the combined approaches of n-alkanes biomarker and bulk parameters were efficient complements in tracing OM source in a hilly-gully region on the Loess Plateau of China.


Assuntos
Alcanos/análise , Lipídeos/análise , Solo/química , Agricultura , Teorema de Bayes , Biomarcadores/análise , Isótopos de Carbono/análise , China , Florestas , Sedimentos Geológicos/química , Pradaria , Isótopos de Nitrogênio/análise
14.
Int Urol Nephrol ; 50(1): 55-61, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28975469

RESUMO

PURPOSE: Luciferase modification of tumour cells enables early and non-invasive imaging to detect tumour growth in situ and could provide sensitive and effective detection of carcinoma during research and therapy. METHODS: Renca cells, a murine renal carcinoma cell line, were infected with lentivirus expressing luciferase to obtain Renca-luc. The proliferation, invasion, and migration of Renca and Renca-luc cell lines were compared using colorimetric, Boyden chamber, and wound-healing assays. Orthotopic tumour models were established in BALB/c mice using Renca and Renca-luc cells, and tumour growth in vivo was detected using bioluminescence imaging and magnetic resonance imaging (MRI). RESULTS: Intensity of luciferase signals from Renca-luc was positively correlated with cell number. Bioluminescence signal was detected 1 day after the establishment of the renal carcinoma model using Renca-luc and was significantly increased after 7 days. Tumour size at 7 days following the establishment of renal carcinoma models using Renca and Renca-luc was determined using MRI. The presence of renal model tumours was confirmed by histological staining. The expression of luciferase did not affect Renca cell characteristics in vitro or tumour growth in vivo. CONCLUSION: Luciferase labelling could provide a sensitive and non-invasive evaluation method for immunological and tumour therapy of renal carcinomas.


Assuntos
Carcinoma de Células Renais/metabolismo , Proteínas de Fluorescência Verde/metabolismo , Neoplasias Renais/metabolismo , Luciferases/metabolismo , Luminescência , Animais , Carcinoma de Células Renais/diagnóstico por imagem , Carcinoma de Células Renais/genética , Carcinoma de Células Renais/patologia , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Modelos Animais de Doenças , Vetores Genéticos , Proteínas de Fluorescência Verde/genética , Neoplasias Renais/diagnóstico por imagem , Neoplasias Renais/genética , Neoplasias Renais/patologia , Lentivirus , Luciferases/genética , Camundongos , Camundongos Endogâmicos BALB C , Transplante de Neoplasias , Plasmídeos , Carga Tumoral
15.
Zhonghua Wei Chang Wai Ke Za Zhi ; 20(10): 1180-1187, 2017 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-29130235

RESUMO

OBJECTIVE: To investigate the risk factors of postoperative perineal wound complications after abdominoperineal resection for rectal cancer. METHODS: The databases of Medline, Embase, Web of Science, Ovid, Cochrane Library, CBM, CNKI, VIP and WANFANG were searched for the studies of abdominoperineal resection up to October 2016. The quality of the included studies was assessed by using "Cochrane collaboration's tool for assessing risk of bias" and "the Newcastle-Ottawa Scale". The meta-analyses were performed with Review Manager 4.3 software. RESULTS: Eight randomized controlled trials and 33 non-randomized controlled trials with 15 287 patients were enrolled. Meta-analyses showed that neoadjuvant radiotherapy (OR=2.55, 95%CI: 1.66 to 3.93, P<0.01) and obesity (OR=2.12, 95%CI: 1.05 to 4.26, P=0.04) significantly increased the morbidity of perineal wound complication after abdominoperineal resection for rectal cancer; omentoplasty(OR=0.30, 95%CI: 0.14 to 0.67, P=0.003), presacral space clysis (OR=0.11, 95%CI: 0.01 to 0.94, P=0.04), abdominal drainage (OR=0.36, 95%CI: 0.21 to 0.63, P<0.01), perineal skin drainage(OR=41.72, 95%CI: 2.39 to 727.90, P=0.01) and local application of antibiotics (OR=0.17,95%CI: 0.07 to 0.40, P<0.01) significantly decreased the morbidity of perineal wound complication; however, extralevator abdominoperineal excision (OR=0.88, 95%CI: 0.57 to 1.35, P=0.56), laparoscopic procedure (OR=1.02, 95%CI: 0.47 to 2.21, P=0.96), biologic mesh reconstruction (OR=1.81, 95%CI: 0.95 to 3.46, P=0.07), myocutaneous flap reconstruction (OR=1.32, 95%CI: 0.18 to 9.91, P=0.79) and negative pressure drainage(OR=0.69, 95%CI: 0.35 to 1.34, P=0.27) had no influence on the healing of perineal wound. CONCLUSIONS: Numerous factors can affect the occurrence of perineal wound complication after abdominoperineal resection for rectal cancer. Due to the limitations of enrolled studies, multicenter large scale and high-quality randomized controlled trials are required to validate the current results.


Assuntos
Períneo/cirurgia , Complicações Pós-Operatórias , Neoplasias Retais/cirurgia , Infecção da Ferida Cirúrgica , Abdome , Humanos , Períneo/patologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Reto , Fatores de Risco
16.
J Dent ; 66: 76-82, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28826985

RESUMO

OBJECTIVES: Dental luting cements are widely used to bond indirect restorations to teeth. Microcracks often lead to cement failures. The objectives of this study were to develop the first self-healing luting cement, and investigate dentin bond strength, mechanical properties, crack-healing, and self-healing durability in water-aging for 6 months. METHODS: Microcapsules of poly(urea-formaldehyde) (PUF) shells with triethylene glycol dimethacrylate (TEGDMA) as healing liquid were synthesized. Cement contained bisphenol A glycidyl dimethacrylate, TEGDMA, 4-methacryloyloxyethyl trimellitic and glass fillers. Microcapsules were added at 0%, 2.5%, 5%, 7.5%, 8.5%, 9.5% and 10%. Dentin shear bond strength was measured using extracted human teeth. Flexural strength and elastic modulus were measured. Single edge V-notched beams were used to measure fracture toughness (KIC) and self-healing. Specimens were water-aged at 37°C for 6 months and then tested for self-healing durability. RESULTS: Adding 7.5% microcapsules into cement achieved effective self-healing, without adverse effects on dentin bond strength and virgin mechanical properties (p>0.1). Excellent self-healing of 68%-77% recovery was obtained. Six months of water-aging did not decrease the self-healing efficiency, compared to 1 d (p>0.1), indicating that the self-healing property did not degrade in water-aging. CONCLUSIONS: A self-healing dental luting cement was developed for the first time. It contained fine microcapsules and exhibited an excellent self-healing efficiency, even after being immersed in water for 6 months. CLINICAL SIGNIFICANCE: The self-healing cement is promising for cementing crowns and bridges and other adhesive cement applications, to heal cracks and increase restoration longevity.


Assuntos
Cápsulas/química , Cimentos Dentários/química , Restauração Dentária Permanente , Teste de Materiais , Compostos Benzidrílicos , Bis-Fenol A-Glicidil Metacrilato/química , Resinas Compostas/química , Colagem Dentária , Materiais Dentários/química , Falha de Restauração Dentária , Módulo de Elasticidade , Vidro , Cimentos de Ionômeros de Vidro/química , Humanos , Metacrilatos/química , Polietilenoglicóis/química , Polimerização , Ácidos Polimetacrílicos/química , Resistência à Tração , Água/química
17.
Dent Mater ; 33(9): 1033-1044, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28734567

RESUMO

OBJECTIVE: Secondary caries is a primary reason for dental restoration failures. The objective of this study was to investigate the remineralization of human dentin lesions in vitro via restorations using nanocomposites containing nanoparticles of amorphous calcium phosphate (NACP) or NACP and tetracalcium phosphate (TTCP) for the first time. METHODS: NACP was synthesized by a spray-drying technique and incorporated into a resin consisting of ethoxylated bisphenol A dimethacrylate (EBPADMA) and pyromellitic glycerol dimethacrylate (PMGDM). After restoring the dentin lesions with nanocomposites as well as a non-releasing commercial composite control, the specimens were treated with cyclic demineralization (pH 4, 1h per day) and remineralization (pH 7, 23h per day) for 4 or 8 weeks. Calcium (Ca) and phosphate (P) ion releases from composites were measured. Dentin lesion remineralization was measured at 4 and 8 weeks by transverse microradiography (TMR). RESULTS: Lowering the pH increased ion release of NACP and NACP-TTCP composites. At 56 days, the released Ca concentration in mmol/L (mean±SD; n=3) was (13.39±0.72) at pH 4, much higher than (1.19±0.06) at pH 7 (p<0.05). At 56 days, P ion concentration was (5.59±0.28) at pH 4, much higher than (0.26±0.01) at pH 7 (p<0.05). Quantitative microradiography showed typical subsurface dentin lesions prior to the cyclic demineralization/remineralization treatment, and dentin remineralization via NACP and NACP-TTCP composites after 4 and 8 weeks of treatment. At 8 weeks, NACP nanocomposite achieved dentin lesion remineralization (mean±SD; n=15) of (48.2±11.0)%, much higher than (5.0±7.2)% for dentin in commercial composite group after the same cyclic demineralization/remineralization regimen (p<0.05). SIGNIFICANCE: Novel NACP-based nanocomposites were demonstrated to achieve dentin lesion remineralization for the first time. These results, coupled with acid-neutralization and good mechanical properties shown previously, indicate that the NACP-based nanocomposites are promising for restorations to inhibit caries and protect tooth structures.


Assuntos
Resinas Compostas , Dentina , Nanocompostos , Fosfatos de Cálcio , Cariostáticos , Humanos , Desmineralização do Dente , Remineralização Dentária
18.
Sci Rep ; 7(1): 2296, 2017 05 23.
Artigo em Inglês | MEDLINE | ID: mdl-28536451

RESUMO

Better understanding the drug action within cells may extend our knowledge on drug action mechanisms and promote new drugs discovery. Herein, we studied the processes of drug induced chemical changes on proteins and nucleic acids in human breast adenocarcinoma (MCF-7) cells via time-resolved plasmonic-enhanced Raman spectroscopy (PERS) in combination with principal component analysis (PCA). Using three popular chemotherapy drugs (fluorouracil, cisplatin and camptothecin) as models, chemical changes during drug action process were clearly discriminated. Reaction kinetics related to protein denaturation, conformational modification, DNA damage and their associated biomolecular events were calculated. Through rate constants and reaction delay times, the different action modes of these drugs could be distinguished. These results may provide vital insights into understanding the chemical reactions associated with drug-cell interactions.


Assuntos
Antineoplásicos/farmacologia , Desnaturação Proteica/efeitos dos fármacos , Análise de Célula Única/métodos , Análise Espectral Raman/métodos , Adenocarcinoma/genética , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Camptotecina/farmacologia , Fenômenos Químicos , Cisplatino/farmacologia , Feminino , Fluoruracila/farmacologia , Ouro/química , Humanos , Cinética , Células MCF-7 , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Análise de Componente Principal
19.
Int Urol Nephrol ; 49(6): 967-974, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28247169

RESUMO

OBJECTIVE: To investigate the role of neutrophil-to-lymphocyte ratio as a prognostic indicator in patients with localized clear cell renal cell carcinoma treated with radiofrequency ablation. PATIENTS AND METHODS: We retrospectively analyzed data from patients with renal cell carcinoma who underwent radiofrequency ablation from 2006 to 2013. The Kaplan-Meier method was used to generate the survival curves according to different categories of neutrophil-to-lymphocyte ratio. Relationships between preoperative neutrophil-to-lymphocyte ratio or the change of neutrophil-to-lymphocyte ratio and survival were evaluated with multivariable Cox proportional hazards regression analysis. A propensity score matching analysis was carried out to avoid confounding bias. RESULTS: A total of 185 patients were included in present study. When stratified by preoperative neutrophil-to-lymphocyte ratio cutoff value of 2.79, 5-year recurrence-free survival, 5-year disease-free survival, and 5-year overall survival rates of neutrophil-to-lymphocyte ratio <2.79 versus ≥2.79 were 100, 98.5, and 99.2% versus 80.5, 72.6, and 90.6%, respectively (P < 0.001, P < 0.001, P = 0.003). In terms of propensity score matching analysis, 5-year recurrence-free survival, 5-year disease-free survival, and 5-year overall survival rates of neutrophil-to-lymphocyte ratio <2.79 versus ≥2.79 were 100, 97.9, and 100% versus 82.3, 73.4, and 89.4%, respectively (P = 0.003, P = 0.001, P = 0.022). When combining preoperative neutrophil-to-lymphocyte ratio with the change of neutrophil-to-lymphocyte ratio, patients with both preoperative neutrophil-to-lymphocyte ratio ≥2.79 and the change of neutrophil-to-lymphocyte ratio ≥0.40 had the worst disease-free survival. Results of multivariable analysis showed that preoperative neutrophil-to-lymphocyte ratio and the change of neutrophil-to-lymphocyte ratio correlated with cancer relapse remarkably. CONCLUSIONS: High preoperative neutrophil-to-lymphocyte ratio and elevated postoperative neutrophil-to-lymphocyte ratio are associated with significant increase in risk of local recurrence as well as distant metastasis. The combination of neutrophil-to-lymphocyte ratio with the other prognostic indicators can be applied in the evaluation of relapse risk in patients with clear cell renal cell carcinoma after radiofrequency ablation.


Assuntos
Carcinoma de Células Renais/sangue , Neoplasias Renais/sangue , Linfócitos , Recidiva Local de Neoplasia/sangue , Neutrófilos , Idoso , Área Sob a Curva , Carcinoma de Células Renais/patologia , Carcinoma de Células Renais/cirurgia , Ablação por Cateter , Intervalo Livre de Doença , Feminino , Seguimentos , Humanos , Estimativa de Kaplan-Meier , Neoplasias Renais/patologia , Neoplasias Renais/cirurgia , Contagem de Linfócitos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Período Pré-Operatório , Pontuação de Propensão , Curva ROC , Estudos Retrospectivos , Taxa de Sobrevida
20.
ACS Nano ; 11(3): 2872-2885, 2017 03 28.
Artigo em Inglês | MEDLINE | ID: mdl-28240869

RESUMO

The exciting commercial application potential of graphene materials may inevitably lead to their increasing release into the environment where they may pose ecological risks. This study focused on using carbon-14-labeled few-layer graphene (FLG) to determine whether the size of graphene plays a role in its uptake, depuration, and biodistribution in adult zebrafish. After 48 h exposure to larger FLG (L-FLG) at 250 µg/L, the amount of graphene in the organism was close to 48 mg/kg fish dry mass, which was more than 170-fold greater than the body burden of those exposed to the same concentration of smaller FLG (S-FLG). The amount of uptake for both L-FLG and S-FLG increased by a factor of 2.5 and 16, respectively, when natural organic matter (NOM) was added in the exposure suspension. While the L-FLG mainly accumulated in the gut of adult zebrafish, the S-FLG was found in both the gut and liver after exposure with or without NOM. Strikingly, the S-FLG was able to pass through the intestinal wall and enter the intestinal epithelial cells and blood. The presence of NOM increased the quantity of S-FLG in these cells. Exposure to L-FLG or S-FLG also had a significantly different impact on the intestinal microbial community structure.


Assuntos
Antibacterianos/farmacologia , Trato Gastrointestinal/química , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Grafite/farmacologia , Mucosa Intestinal/química , Fígado/química , Aeromonas/efeitos dos fármacos , Animais , Antibacterianos/química , Bactérias/efeitos dos fármacos , Radioisótopos de Carbono , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Grafite/química , Mucosa Intestinal/microbiologia , Testes de Sensibilidade Microbiana , Tamanho da Partícula , Relação Estrutura-Atividade , Propriedades de Superfície , Distribuição Tecidual , Peixe-Zebra
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