Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Mol Med Rep ; 20(4): 3182-3190, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31432137

RESUMO

Atherosclerosis is a chronic inflammatory disease, and it is a global clinical problem. The development of new and effective therapeutic targets for atherosclerosis is necessary. A number of microRNAs (miRNAs) have been demonstrated to serve a crucial role in atherosclerosis. However, the role of miRNA (miR)­217 in atherosclerosis remains unclear. Therefore, the aim of the present study was to investigate the role and mechanism of miR­217 in atherosclerosis. The level of miR­217 was detected in the blood of patients with atherosclerosis using reverse transcription­quantitative PCR. THP­1 acute monocytic leukemia cells were treated with oxidized low­density lipoprotein (ox­LDL) to develop an atherosclerotic cell model of macrophages. The relationship between miR­217 and sirtuin 1 (SIRT1) was determined by TargetScan and dual luciferase reporter assay. Cell apoptosis was measured by flow cytometry. Production of pro­inflammatory factors and triglyceride (TG) and total cholesterol (TC) levels were also determined. The results demonstrated that miR­217 was significantly upregulated in atherosclerosis. SIRT1 was demonstrated to be a direct target of miR­217 and was downregulated in atherosclerosis. Downregulation of miR­217 significantly inhibited ox­LDL­induced TG and TC level increase, cell apoptosis and the upregulation of the pro­inflammatory factors tumor necrosis factor α, interleukin (IL)­6 and IL­1ß. Additionally, the SIRT1/AMP­activated protein kinase α/NF­κB pathway was at least partially involved in modulating the effects of miR­217 inhibition on THP­1 cells treated with ox­LDL. In addition, the effects of miR­217 downregulation on ox­LDL­treated THP­1 cells were eliminated by SIRT1 silencing. In conclusion, the results of the present study indicated that miR­217 downregulation may relieve atherosclerosis through the inhibition of macrophage apoptosis and inflammatory response by targeting SIRT1.

2.
Chem Commun (Camb) ; 54(19): 2401-2404, 2018 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-29457188

RESUMO

The control of reaction pathways for selective and enantiospecific synthesis of functionalized pyrrolidines and γ-amino ketones has been realized. Rhodium-catalyzed [3+2] cycloadditions of vinylaziridines and enolsilanes with a bulky silyl group gave functionalized pyrrolidines with moderate to excellent diastereoselectivities, while the reaction of silyl enol ethers with a less bulky silyl group afforded chiral γ-amino ketones in good yields.

3.
Chem Sci ; 8(6): 4660-4665, 2017 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-28936335

RESUMO

The enantioselective construction of densely functionalized cyclopentene bearing contiguous three stereocenters has been a challenging task in organic synthesis. Herein, we present a phoshine-catalyzed highly regio-, diastereo- and enantioselective [3 + 2] cycloaddition of γ-substituted allenoates with ß-perfluoroalkyl enones, delivering a wide range of densely functionalized perfluoroalkylated cyclopentenes with three contiguous chiral stereocenters.

4.
Chem Commun (Camb) ; 53(34): 4688-4691, 2017 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-28401219

RESUMO

An atom-economic route to ring-fused 1,2,4-hexahydrotriazines relying upon a new synthetic application of vinylaziridines in a rhodium-catalyzed intermolecular [3+3] cycloaddition of C,N-cyclic azomethine imines has been reported. Highly efficient chirality transfer in the present transformation was also observed, providing a new method for the synthesis of sp3-carbon-rich 1,2,4-hexahydrotriazines in an enantioselective manner.

5.
Angew Chem Int Ed Engl ; 56(5): 1351-1355, 2017 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-27966804

RESUMO

A new synthetic application of vinyl aziridines as N-containing three-atom components in a rhodium-catalyzed [4+3] cycloaddition reaction is described. The reaction proceeds well with various silyl dienol ethers and vinyl aziridines, and enables the efficient synthesis of highly functionalized azepines in an enantioselective manner with net inversion of absolute configuration. The salient features of the transformation include the use of readily available substrates, high selectivity, and mild reaction conditions, as well as the versatile functionalization of the products.

6.
Angew Chem Int Ed Engl ; 55(36): 10844-8, 2016 08 26.
Artigo em Inglês | MEDLINE | ID: mdl-27485044

RESUMO

The first rhodium-catalyzed intermolecular [3+2] cycloaddition reaction of vinyl aziridines and allenes for the synthesis of enantioenriched functionalized pyrrolidines was realized. [3+2] cycloaddition with the proximal C=C bond of N-allenamides gave 3-methylene-pyrrolidines in high regio- and diastereoselectivity, whereas, 2-methylene-pyrrolidines were obtained as the major products by the cycloadditions of vinyl aziridines with the distal C=C bond of allenes. Use of readily available starting materials, a broad substrate scope, high selectivity, mild reaction conditions, as well as versatile functionalization of the cycloadducts make this approach very practical and attractive.

7.
J Am Chem Soc ; 138(7): 2178-81, 2016 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-26859710

RESUMO

Catalyst-controlled divergent intermolecular cycloadditions of vinylaziridines with alkynes have been developed. By using [Rh(NBD)2]BF4 as the catalyst, a [3 + 2] cycloaddition reaction was achieved with broad substrate scope and high stereoselectivity under mild reaction conditions. Moreover, the chirality of vinylaziridines can be completely transferred to the [3 + 2] cycloadducts. When the catalyst was changed to [Rh(η(6)-C10H8) (COD)]SbF6, the alternative [5 + 2] cycloadducts were selectively formed under otherwise identical conditions.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA