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1.
Rev Med Liege ; 76(9): 689-696, 2021 Sep.
Artigo em Francês | MEDLINE | ID: mdl-34477341

RESUMO

Since the early 1970s, the concept of quality of life has been the subject of increasing interest in the medical field, although no scientific consensus has emerged on how to define and measure it. The aim of this narrative review of the literature is to decrypt the notion of quality of life in the medical field, in order to enable clinicians-researchers and clinicians who use quality of life measurement instruments in clinical practice to form an informed and nuanced opinion on the issue. To do so, the paper is divided into three parts. Firstly, a brief overview of the origin of the concept in the medical field is given by exposing the main factors explaining its emergence and its rise in importance. Next, the plurality of definitions of quality of life and its derivatives (e.g. health-related quality of life), as well as its measurement instruments in the medical field, are explored. Finally, some benchmarks for the use of health-related quality of life instruments in clinical practice are presented.


Assuntos
Qualidade de Vida , Semântica , Humanos
2.
Cell Biochem Biophys ; 78(2): 123-126, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32086746

RESUMO

Honey bees play a crucial role in the nature by pollinating wild flowers. Over the past years, there has been an increasing concern regarding the honey bee colony decline. Pesticides or environmental effects targeting the biochemistry of insect chitin and cuticle coating may be in part responsible for honey bee pathologies. We here propose the use of electron paramagnetic resonance imaging (EPRI) as a tool to image the melanin-chitin complexes as part of the exoskeleton of the honey bee. EPRI at 9.65 GHz was applied on intact freeze-dried bees. The imaging data were collected on the melanin peak. High-resolution images revealed that this compound is extensively distributed in the periphery of the animal, data consistent with the localization in the cuticle of the bee. While EPR of melanin has been so far explored in the context of melanoma characterization, it may offer new opportunities in research on honey bees and other insects.


Assuntos
Abelhas/química , Quitina/análise , Espectroscopia de Ressonância de Spin Eletrônica , Melaninas/análise , Exoesqueleto/química , Animais , Quitina/química , Radicais Livres , Liofilização , Imageamento Tridimensional , Proteínas de Insetos/química , Melaninas/química , Praguicidas
3.
Health Educ Res ; 24(6): 949-56, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19741003

RESUMO

Community participation in program decision-making and implementation is an ideal that community and academic stakeholders aspire to in participatory research. This ideal, however, can be difficult to achieve. We describe lessons learned about community participation from a quasi-experimental trial aimed at reducing the uptake of smoking among pre-adolescents in a community with a high percentage of Maori and Pacific Island people. The intervention involves students, parents, school teachers and management, extended families and members of the wider community. A total of approximately 4000 students (and their parents) of four urban Auckland schools were enrolled in the study over 3 years. The intervention is carried out through collaborations between public health professionals, academic institutions and school personnel. In order to enhance community participation, we conclude that (i) time commitment is needed to establish long-term ongoing relationships through face-to-face communication, (ii) research team members should ideally share similar cultural and ethnic backgrounds to the target audience and have in-depth understanding of and experience in the community milieu and (iii) collaborative partnerships between academic institutions and public health services are necessary to create strength and cohesion, and assist with clear articulation of the research project mission and objectives.


Assuntos
Participação da Comunidade/métodos , Prevenção do Hábito de Fumar , Criança , Grupos Focais , Promoção da Saúde/organização & administração , Humanos , Entrevistas como Assunto , Nova Zelândia , Avaliação de Programas e Projetos de Saúde
4.
J Magn Reson ; 197(2): 176-80, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19128993

RESUMO

This article reports the development and evaluation of two nano-emulsions (F45T-03/HFB and F15T-03/PFOB) containing fluorinated trityl radicals dissolved in perfluorocarbons. Preparation with a high-pressure homogenizer conferred sub-micronic size to both nano-emulsions. In vitro and in vivo EPR spectroscopy showed that the nano-emulsions had much greater oxygen sensitivity than the hydrophilic trityl, CT-03. In vivo experiments in rodents confirmed the ability of the nano-emulsions to follow the changes in oxygen concentration after induced ischemia. Histological evaluation of the tissue injected with the nano-emulsions revealed some acute toxicity for the F45T-03/HFB nano-emulsion but none for the F15T-03/PFOB nano-emulsion. These new formulations should be considered for further EPR oximetry experiments in pathophysiological situations where subtle changes in tissue oxygenation are expected.


Assuntos
Espectroscopia de Ressonância de Spin Eletrônica/métodos , Emulsões , Radicais Livres/química , Nanopartículas/química , Oximetria/métodos , Trítio/química , Animais , Materiais Biocompatíveis , Fluorcarbonetos/química , Radicais Livres/farmacocinética , Masculino , Camundongos , Oximetria/instrumentação , Tamanho da Partícula , Distribuição Tecidual
6.
Transfus Clin Biol ; 9(4): 258-64, 2002 Oct.
Artigo em Francês | MEDLINE | ID: mdl-12469557

RESUMO

The incidence rate of ABO hazards of transfusion remains high in France. In this country, bedside pretransfusion controls include an agglutination test for red cells only, although its validity has scarcely been assessed in the practice. 847 nurses from 9 public hospitals and private clinics in a French region participated in a study aimed at measuring the sensitivity and specificity of pretransfusion bedside agglutination tests within hospital wards. Sensitivity was found to be 93.9% +/- 3%. Nondetection of mismatching was increased by two risk factors only: having worked more than 4 years in the same ward, and not having been trained to use this test. The sensitivity of this test might still be improved. This test is found sensitive enough to be kept. Nevertheless, if used alone, it is not a safe protection against recipient's mismatch. Authors recommend both to improve agglutination test sensitivity and to link it strongly to the bedside checking of both transfusion information and the recipient's identity.


Assuntos
Sistema ABO de Grupos Sanguíneos , Incompatibilidade de Grupos Sanguíneos/prevenção & controle , Transfusão de Sangue/normas , Testes de Hemaglutinação , França , Humanos , Sistemas Automatizados de Assistência Junto ao Leito/normas , Reprodutibilidade dos Testes
7.
Biochim Biophys Acta ; 1517(2): 257-69, 2001 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-11342107

RESUMO

X-linked adrenoleukodystrophy (X-ALD) is an inherited demyelinating disorder due to mutations in the ALD gene, which encodes a peroxisomal ABC half-transporter (ALDP). It has been suggested that ALDP assembles with ALDRP (adrenoleukodystrophy-related protein), a close homologous half-transporter, to form a functional heterodimer. For the first time full-length ALDRP cDNA (5.5 kb) was cloned, and 5' and 3' RACE analysis revealed that alternative usage of polyadenylation sites generates the two transcripts of 3.0 and 5.5 kb observed in the rat in Northern blot analysis. Southern blotting and chromosomal mapping demonstrated one ALDR locus in the rat genome. Characterisation of the 3' flanking region suggested that an ID sequence might be responsible for high expression of the 5.5 kb ALDRP transcript in rat brain. ALDR gene expression was found to be high in the liver of rats before weaning and very low in adult rats; the reverse developmental regulation was observed in the brain. Fenofibrate, which is a potent inducer of the ALDR gene in the liver of adult rats, could not induce the ALDR gene in suckling rats. The exact significance of this result with regard to development of an efficient pharmacological gene therapy for X-ALD is discussed.


Assuntos
Transportadores de Cassetes de Ligação de ATP , Adrenoleucodistrofia/genética , Proteínas/genética , Regiões 3' não Traduzidas/química , Regiões 5' não Traduzidas/química , Subfamília D de Transportador de Cassetes de Ligação de ATP , Sequência de Aminoácidos , Animais , Sequência de Bases , Encéfalo/metabolismo , Mapeamento Cromossômico , Clonagem Molecular , DNA Complementar/biossíntese , DNA Complementar/química , Fenofibrato , Regulação da Expressão Gênica no Desenvolvimento , Biblioteca Gênica , Camundongos , Dados de Sequência Molecular , Biossíntese de Proteínas , Proteínas/química , Ratos , Ratos Wistar
8.
Genomics ; 70(1): 131-9, 2000 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-11087670

RESUMO

The adrenoleukodystrophy-related gene (ALDR, ABCD2) is a candidate modifier gene and a potential therapeutic target for X-linked adrenoleukodystrophy (ALD), a severe neurodegenerative disease. The ALDR gene is the closest homologue of the ALD gene, which encodes a peroxisomal ABC transporter involved in the catabolism of very-long-chain fatty acids. Administration of fenofibrate upregulates ALDR expression in rodent liver. As a step toward understanding ALDR transcriptional regulation, the mouse and human 5' regions were characterized. The human and mouse genes share a 500-bp conserved region that contains potential Sp1- and AP-2-binding sites but no TATA box. Analysis of the 5'-flanking region of ALDR using a luciferase reporter system revealed that 1.3 kb of human or mouse 5'-upstream region has functional promoter activity. In these transfection experiments, promoter activity of both human and mouse genes could be upregulated by 9-cis-retinoic acid and forskolin, while no effect of PPARalpha could be detected.


Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Adrenoleucodistrofia/genética , Colforsina/farmacologia , Regiões Promotoras Genéticas , Proteínas/genética , Tretinoína/farmacologia , Subfamília D de Transportador de Cassetes de Ligação de ATP , Animais , Sequência de Bases , AMP Cíclico/análogos & derivados , Regulação da Expressão Gênica , Humanos , Camundongos , Dados de Sequência Molecular , Receptores Citoplasmáticos e Nucleares/metabolismo , Fatores de Transcrição/metabolismo
9.
Hum Hered ; 50(5): 304-7, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10878474

RESUMO

Polymorphisms of the chemokine receptor genes CCR5 and CCR2 are associated with resistance to HIV-1 infection or delayed progression to AIDS. Few data are available on their combined prevalence in healthy subjects; we therefore examined the occurrence of CCR5-Delta32 and CCR2-64I polymorphisms in a sample of 310 healthy Belgians. Allele frequencies were 0.119 and 0.074 for CCR5-Delta32 and CCR2-64I, respectively. Genotype distributions for both polymorphisms were found to be in accordance with Hardy-Weinberg equilibrium, but a significant (p = 0.002) linkage disequilibrium between CCR5-Delta32 and CCR2-64I was observed. The high prevalence of CCR5-Delta32 and CCR2-64I in Belgians may need to be taken into account in the design of studies of antiretroviral treatments.


Assuntos
HIV-1 , Polimorfismo Genético , Receptores CCR5/genética , Receptores de Quimiocinas , Receptores de Citocinas/genética , Bélgica , Grupo com Ancestrais do Continente Europeu/genética , Frequência do Gene , Genótipo , Humanos , Prevalência , Receptores CCR2
10.
J Leukoc Biol ; 67(1): 81-9, 2000 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-10648001

RESUMO

We tested the hypothesis of a conserved activation mode of monocytic THP-1 cells by proteins I/II expressed by several species of oral streptococci through the specific role of the extended V-region. We studied the binding and modulating activities of six proteins I/II purified from strains representing four different species of oral streptococci, and of expression products of polymerase chain reaction-amplified sequences encoding corresponding extended V-regions. We found that the different proteins I/II bound to THP-1 cells in a sugar-dependent mode involving the extended V-region. Furthermore, all the proteins I/II stimulated THP-1 cells to produce tumor necrosis factor alpha, indicating that these properties are not strain- or species-specific. Despite the weak stimulation of THP-1 cells by the extended V-region alone, we obtained evidence that cross-linking of this region can be one of the mechanisms involved in monocytic cell activation by proteins I/II.


Assuntos
Proteínas de Bactérias/fisiologia , Glicoproteínas de Membrana , Monócitos/microbiologia , Monócitos/fisiologia , Fator de Necrose Tumoral alfa/metabolismo , Sequência de Aminoácidos , Antígenos de Bactérias/química , Antígenos de Bactérias/fisiologia , Proteínas de Bactérias/química , Linhagem Celular , Reagentes para Ligações Cruzadas , Humanos , Dados de Sequência Molecular , Infecções Estreptocócicas/fisiopatologia
11.
Eur J Cell Biol ; 75(3): 254-64, 1998 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9587057

RESUMO

The adrenoleukodystrophy and adrenoleukodystrophy related proteins belong to a new family of half ATP-binding cassette transporters which are localized within the peroxisomal membrane and whose functions are still unknown. They could possibly homo- or heterodimerize resulting in transporters with similar or distinct functions. The expression of adrenoleukodystrophy and adrenoleukodystrophy related genes was studied at the mRNA and protein levels in adult mouse tissues and several human cell lines. We found that adrenoleukodystrophy and adrenoleukodystrophy related genes have strikingly different expression in most mouse tissues and human cell lines analyzed, indicating that adrenoleukodystrophy and adrenoleukodystrophy related proteins do not function as obligatory partners but might rather fulfill similar metabolic functions in different tissues.


Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Expressão Gênica , Proteínas de Membrana/genética , Proteínas/genética , Subfamília D de Transportador de Cassetes de Ligação de ATP , Membro 1 da Subfamília D de Transportadores de Cassetes de Ligação de ATP , Transportadores de Cassetes de Ligação de ATP/metabolismo , Animais , Encéfalo/metabolismo , Humanos , Imuno-Histoquímica , Masculino , Proteínas de Membrana/metabolismo , Camundongos , Proteínas/metabolismo , RNA Mensageiro , Retina/metabolismo , Testículo/metabolismo , Células Tumorais Cultivadas
12.
Eur J Hum Genet ; 6(6): 638-41, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-9887385

RESUMO

ALDR is one of the four genes encoding an ATP Binding Cassette (ABC) hemi-transporter of the peroxisomal membrane so far identified in mammalian cells. The best known of these is X-ALD, whose dysfunction has been causally associated with X-linked adrenoleukodystrophy. ALDR and X-ALD protein product are closely related and we show here that this striking conservation is maintained at the genomic level. Although extending to a larger genomic region, the organisation of the mouse ALDR gene mirrors exactly that of X-ALD. This supports further the hypothesis that among the four known peroxisomal ABC hemi-transporters ALDRP is the most likely candidate as a modifier contributing to the phenotypic variability of X-linked adrenoleukodystrophy.


Assuntos
Transportadores de Cassetes de Ligação de ATP , Adrenoleucodistrofia/genética , Éxons , Proteínas/genética , Subfamília D de Transportador de Cassetes de Ligação de ATP , Sequência de Aminoácidos , Animais , Sequência de Bases , DNA Complementar , Camundongos , Dados de Sequência Molecular , Mapeamento por Restrição , Homologia de Sequência de Aminoácidos , Cromossomo X
13.
Eur J Hum Genet ; 5(2): 99-101, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9195160

RESUMO

We report here on the chromosomal mapping of the adrenoleukodystrophy-related (ALDR) gene on both the human and the mouse genomes. This gene encodes a peroxisomal ATP binding cassette transporter, closely related to the transporter identified as responsible for the adrenoleukodystrophy phenotype. ALDR maps on the syntenic region on murine and human autosomes. In addition, we could determine its position in relation to known microsatellite framework markers; this will allow to test its role in Zellweger syndrome and/ or related peroxisomal disorders.


Assuntos
Transportadores de Cassetes de Ligação de ATP , Adrenoleucodistrofia/genética , Proteínas/genética , Subfamília D de Transportador de Cassetes de Ligação de ATP , Animais , Mapeamento Cromossômico , Cromossomos/genética , Cromossomos Humanos Par 12/genética , Humanos , Hibridização In Situ , Proteínas de Membrana/genética , Camundongos , Transtornos Peroxissômicos/genética , Síndrome de Zellweger/genética
14.
J Immunol ; 152(5): 2447-55, 1994 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-8133056

RESUMO

Cefdinir, a new oral 2-amino-5-thiazolyl cephalosporin, inhibited the luminol-amplified chemiluminescence (LACL) response of human neutrophils stimulated by PMA but not opsonized zymosan, in a concentration-dependent but not time-dependent manner. The LACL response to opsonized zymosan in cytochalasin B-treated neutrophils was, however, inhibited by cefdinir. Various cephalosporins, regardless of the presence of a 2-amino-5-thiazolyl moiety, did not significantly alter the neutrophil LACL response triggered by PMA and zymosan. The LACL response induced by the calcium ionophore A23187 and FMLP was also impaired by cefdinir, and this impairment was increased in cytochalasin B-treated neutrophils. Superoxide anion generation by neutrophils, measured in terms of lucigenin-amplified chemiluminescence and cytochrome c reduction, was not altered. Spontaneous and FMLP-induced neutrophil degranulation, assessed by lysozyme and beta-glucuronidase release, were not modified by cefdinir. Furthermore, cefdinir inhibited LACL generation in cell-free systems consisting of H2O2, NaI, and either horseradish peroxidase or a myeloperoxidase-containing neutrophil extract. Orthodianisidine oxidation in these two acellular systems was inhibited by cefdinir. Cefdinir did not alter neutrophil bacterial killing at concentrations that inhibited myeloperoxidase-containing neutrophil extract-dependent reactions induced by soluble stimuli. Taken together, these data strongly suggest that cefdinir directly inhibits the activity of myeloperoxidase-containing neutrophil extract released into the extracellular medium during neutrophil stimulation by soluble mediators, but has no effect on that released into the phagolysosome during phagocytosis. This unusual property of a member of the beta-lactam family could be of interest in modulating the exaggerated inflammatory process often associated with infectious diseases.


Assuntos
Cefalosporinas/farmacologia , Neutrófilos/efeitos dos fármacos , Neutrófilos/enzimologia , Peroxidase/antagonistas & inibidores , Atividade Bactericida do Sangue/efeitos dos fármacos , Calcimicina/farmacologia , Cefdinir , Degranulação Celular/efeitos dos fármacos , Citocalasina B/farmacologia , Espaço Extracelular/enzimologia , Humanos , Peróxido de Hidrogênio/metabolismo , Técnicas In Vitro , Medições Luminescentes , N-Formilmetionina Leucil-Fenilalanina/farmacologia , Neutrófilos/fisiologia , Fagossomos/enzimologia , Acetato de Tetradecanoilforbol/farmacologia , Zimosan/farmacologia
15.
BMJ ; 305(6859): 921-5, 1992 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-1458072

RESUMO

OBJECTIVES: To compare the tolerance, efficacy, and pharmacokinetics of amphotericin deoxycholate (Fungizone) prepared in a parenteral fat emulsion (Intralipid 20%) or glucose in HIV patients with candidiasis. DESIGN: Non-blind randomised controlled trial. SETTING: University hospital; tertiary clinical care. PATIENTS: 22 HIV positive patients with oral candidiasis. INTERVENTIONS: Amphotericin 1 mg/kg/day given on four consecutive days as a one hour infusion dissolved in either 5% glucose (amphotericin-glucose) or parenteral fat emulsion at a final concentration of 2 g/l fat emulsion (amphotericin-fat emulsion). MAIN OUTCOME MEASURES: Clinical tolerance (fever, chills, sweats, nausea, arterial pressure, and pulse rate); biological tolerance (serum creatinine, electrolyte, and magnesium values); clinical score of candidiasis; and serum concentrations of amphotericin. RESULTS: 11 patients were enrolled in each group. All the amphotericin-fat emulsion infusions were given without serious problem whereas four amphotericin-glucose infusions were stopped because of renal impairment (n = 3) or severe chills (n = 2), or both. For patients completing the amphotericin-glucose treatment creatine concentration increased by 42 mumol/l; four of seven patients had at least one creatinine value > or = 133 mumol/l versus one of 11 receiving amphotericin-fat emulsion. Magnesium concentration fell significantly with amphotericin-glucose but not with amphotericin-fat emulsion. Clinical side effects were noted in 36/38 infusions with amphotericin-glucose but 10/44 with amphotericin-fat emulsion. Oral candidiasis score was reduced similarly in both groups. Serum amphotericin concentrations were significantly lower and the volume of distribution of the drug higher after infusion of amphotericin-fat emulsion than after amphotericin-glucose. CONCLUSIONS: Clinical and renal toxicity of amphotericin are reduced when the drug is prepared in fat emulsion. Preparation is simple and cost effective. Its efficacy is similar to that of conventional amphotericin.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico , Anfotericina B/uso terapêutico , Antifúngicos/uso terapêutico , Candidíase Bucal/tratamento farmacológico , Ácido Desoxicólico/uso terapêutico , Infecções por HIV/complicações , Infecções Oportunistas Relacionadas com a AIDS/complicações , Adolescente , Adulto , Idoso , Anfotericina B/administração & dosagem , Anfotericina B/farmacocinética , Antifúngicos/administração & dosagem , Antifúngicos/farmacocinética , Candidíase Bucal/complicações , Ácido Desoxicólico/administração & dosagem , Ácido Desoxicólico/farmacocinética , Combinação de Medicamentos , Excipientes , Emulsões Gordurosas Intravenosas , Glucose/administração & dosagem , Humanos , Infusões Intravenosas , Pessoa de Meia-Idade
16.
Pathol Biol (Paris) ; 40(5): 507-12, 1992 May.
Artigo em Francês | MEDLINE | ID: mdl-1495835

RESUMO

Toxic effects limit the use of amphotericin B (AmB) for the treatment of systemic Candida infections. In vitro and in vivo toxicity can be substantially reduced by mixing AmB with a lipid emulsion used for parenteral nutrition, intralipid 20% (IL). This study was designed to evaluate the potential effects of IL on the activity of Amphotericin B against Candida. A clinical strain of Candida albicans was used. AmB deoxycholate (Fungizone) was reconstituted in a 5% glucoce solution (AmB-G5), in 3 mg/ml IL (AmB-IL3) or in 1.5 mg/ml IL (AmB-IL 1.5). Minimum inhibitory concentrations and minimum lethal concentrations were 0.4 mg/l and 2.5 mg/l, respectively, with AmB-G5, 0.1 mg/l and 1 mg/l with AmB-IL3, and 0.24 mg/l and 1 mg/l with AmB-IL 1.5. In vitro killing curves with 0.1 mg/l, 0.25 mg/l, and 2.5 mg/l AmB were determined with the following results: 1) with 0.1 and 0.25 mg/l AmB, fungicidal activity was seen with AmB-IL3 and AmB-IL 1.5 but not with AmB-G5; 2) with 2.5 mg/l AmB, fungicidal activity was less marked with AmB-G5 (-1.7 log CFU/ml after 24 hours) than with AmB-IL3 and AmB-IL1.5 (-4.3 log CFU/ml and -4.2 log CFU/ml, respectively, after 24 hours; p less than 0.05). In rabbits given a single intravenous injection of 4 mg/kg AmB, analysis of infected subcutaneous fibrin clots detected measurable concentrations of AmB beyond the 24th-36th hour, with levels of 0.5 mg/l for AmB-G5 and 1 mg/l for the two AmB-IL preparations over a period of three days.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Anfotericina B/farmacologia , Candida albicans/efeitos dos fármacos , Candidíase/tratamento farmacológico , Lipídeos , Anfotericina B/administração & dosagem , Anfotericina B/envenenamento , Anfotericina B/uso terapêutico , Animais , Candida albicans/isolamento & purificação , Candidíase/microbiologia , Combinação de Medicamentos , Solução Hipertônica de Glucose , Técnicas In Vitro , Injeções Intravenosas , Coelhos
17.
Biol Cell ; 68(3): 205-11, 1990.
Artigo em Inglês | MEDLINE | ID: mdl-1695857

RESUMO

The ovine embryo produces an interferon named ovine Trophoblastin (oTP) which is involved in the maternal recognition of pregnancy and ensures the maintenance of progesterone secretion by the corpus luteum. We have used indirect immunohistofluorescence and in situ hybridization on histological sections to investigate the fate of this protein and its mRNA in ovine embryos from days 3 to 25 of pregnancy. The level of expression was measured by image analysis of the autoradiographs after in situ hybridization. Both techniques clearly demonstrated that oTP and its mRNA were specifically localized in the extra-embryonic trophoblast. Neither the embryonic cells, nor the yolk sac or the amniotic tissues produced the protein or its mRNA. The protein could be detected by d 11 of pregnancy in the elongated blastocyst. Maximum of expression is observed at d 14 and the level decreased by d 16 of pregnancy. The arrest of expression occurred in the regions of trophoblast which have established cellular contacts with the uterine epithelium during the implantation process.


Assuntos
Embrião de Mamíferos/metabolismo , Interferon Tipo I , Interferons/metabolismo , Luteolíticos/antagonistas & inibidores , Proteínas da Gravidez/metabolismo , Prenhez/metabolismo , RNA Mensageiro/metabolismo , Animais , Embrião de Mamíferos/citologia , Endométrio/citologia , Endométrio/metabolismo , Feminino , Imunofluorescência , Interferons/genética , Hibridização de Ácido Nucleico , Gravidez , Proteínas da Gravidez/genética , RNA Mensageiro/genética , Ovinos , Trofoblastos/citologia , Trofoblastos/metabolismo
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