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1.
Aging (Albany NY) ; 13(18): 22490-22501, 2021 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-34560673

RESUMO

The progression of osteoarthritis (OA) is mediated by adipokines, one of which is nesfatin-1, which is responsible for the production of inflammatory cytokines. However, how this molecule may affect the synthesis of the proinflammatory cytokine interleukin 1 beta (IL-1ß) in OA is unclear. Our analyses of records from the Gene Expression Omnibus (GEO) dataset and clinical specimens of synovial tissue revealed higher levels of nesfatin-1 and IL-1ß in OA samples compared with normal healthy tissue. We found that nesfatin-1 facilitates IL-1ß synthesis in human OA synovial fibroblasts (OASFs) and suppresses the generation of micro-RNA (miR)-204-5p, as the miR-204-5p levels in OA patients were lower than those in healthy controls. Nesfatin-1-induced stimulation of IL-1ß in human OASFs occurred via the suppression of miR-204-5p synthesis by the PI3K, Akt, AP-1 and NF-κB pathways. We suggest that nesfatin-1 is worth targeting in OA treatment.

2.
Medicine (Baltimore) ; 100(31): e26757, 2021 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-34397819

RESUMO

ABSTRACT: The role of cognitive, social and biological factors in the etiology of chronic periodontitis has been reported.The aim of this study was to evaluate the salivary cortisol level and interleukin-1 B level in patients of Chronic periodontitis in smokers and stress and nonsmokers without stress.The design of study randomized, prospective, double-blinded, and prospective study.The total sample size was comprised of 600 subjects between the ages of 20 and 50 years. The sample size was divided into 300 males and 300 females. Out of 600 subjects, 200 subjects comprised of subjects with chronic periodontitis with positive depression level with a history of smoking (Group I), 200 subjects comprised of subjects with chronic periodontitis without depression and without smoking (Group II), and 200 subjects who were taken as the control group comprised of healthy subjects without chronic periodontitis, without depression level, and no smoking history (Group III). Salivary cortisol levels were determined by enzyme-linked immunosorbent assay (ELISA).The result showed that there was a positive correlation between morning and evening salivary cortisol level in all the groups with correlation coefficient. There was significant higher value of salivary cortisol in Group I patients when compared with Group II and Group III. However, when the comparison of salivary cortisol levels was done between the Group II and Control group, the result showed nonsignificant P value.It is suggested that stress is positively correlated with the salivary cortisol levels in smokers and nonsmokers.


Assuntos
Periodontite Crônica/sangue , Hidrocortisona/análise , Interleucina-1beta/análise , Adulto , Biomarcadores/análise , Biomarcadores/sangue , Periodontite Crônica/diagnóstico , Periodontite Crônica/epidemiologia , Feminino , Voluntários Saudáveis , Humanos , Hidrocortisona/sangue , Interleucina-1beta/sangue , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Saliva/enzimologia , Fumar/efeitos adversos , Fumar/epidemiologia , Fumar/psicologia , Estresse Psicológico/epidemiologia , Estresse Psicológico/psicologia
4.
Org Lett ; 21(8): 2772-2776, 2019 04 19.
Artigo em Inglês | MEDLINE | ID: mdl-30964693

RESUMO

The first homogeneous and heterogeneous nanoparticle gold-catalyzed addition of sulfur nucleophiles to alkynes was developed. More specifically, gold-catalyzed hydrothiocyanation of haloalkynes gave good yields and good stereoselectivity of vinyl thiocyanates. Furthermore, a sulfur-based gold catalyst (PPh3AuSCN) has shown a unique reactivity in gold-catalyzed reactions such as the cyclization of N-propargylic amides.

5.
Gynecol Minim Invasive Ther ; 7(3): 136-138, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30254958

RESUMO

Hydrosalpinx in reproductive-age women without sexual experience is uncommon and may be asymptomatic. Hydrosalpinx-induced torsion of the adnexa is a rare situation and requires prompt and accurate management. This report describes a 21-year-old female without history of sexual intercourse presented with right lower abdominal pain. Ultrasonography revealed right ovary about 4.2 cm × 3.6 cm in size with a well-defined, round cystic mass measuring 6.3 cm × 4.1 cm without septations. Diagnostic laparoscopic surgery showed a darkish right ovary and round-shaped cystic right hydrosalpinx that twisted at the infundibulopelvic ligament. Laparoscopic detorsion and salpingostomy were performed successfully. This case is a rare gynecologic situation with hydrosalpinx induced adnexa torsion, making it a rare presentation. Early diagnosis is important for preventing ovarian gangrene. Laparoscopy is a useful tool for diagnosing and treating tubal and ovarian torsion. It brings good prognosis if detorsion was performed within 24 h.

6.
Org Biomol Chem ; 16(38): 6918-6922, 2018 10 03.
Artigo em Inglês | MEDLINE | ID: mdl-30225506

RESUMO

An efficient synthesis of α-amino ketones is developed using the umpolung strategy. Umpoled enolates such as N-alkenoxypyridinium salts react smoothly with diverse amines to give α-amino ketones via an SN2' pathway. This umpolung strategy overcomes the drawbacks of traditional methods such as the need for prefunctionalized ketone derivatives. Our method also offers good chemical yields and high functional group tolerance.

7.
Talanta ; 170: 350-357, 2017 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-28501180

RESUMO

A sensitive and stable bioassay for the detection of Aß oligomer (Aßo), a potentially promising candidate biomarker for Alzheimer's disease (AD) diagnosis, was developed using Fe3O4 magnetic nanoparticles (MNPs) as the recognition and concentration elements and BaYF5:Yb,Er upconversion nanoparticles (UCNPs) as highly sensitive labels, conjugated with the Aßo aptamer (DNA1) and the complementary oligonucleotide of the Aßo aptamer (DNA2), respectively. The DNA1 hybridized with DNA2 to form the duplex structure on the surface of the MNPs/UCNPs nanocomposites probe. When the target Aßo was introduced, the aptamer DNA1 preferentially bound with Aßo and caused the dissociation of some complementary DNA2, liberating some UCNP-labeled complementary DNA2 and leading to a decreased upconversion fluorescent intensity on the surface of MNPs. The decreased fluorescence intensity of UCNPs was related to the concentration of Aßo in the range of 0.2-15nM with a detection limit of 36 pM. The developed method then was successfully applied to measure Aßo in artificial cerebrospinal fluid. Benefiting from the magnetic separation and concentration effect of MNPs, the high sensitivity of UCNPs, as well as the selectivity and stability of the aptamer, the present strategy offered valuable information related to early diagnosis of AD process.


Assuntos
Peptídeos beta-Amiloides/líquido cefalorraquidiano , Aptâmeros de Nucleotídeos/química , Bário/química , Técnicas Biossensoriais/métodos , Corantes Fluorescentes/química , Elementos da Série dos Lantanídeos/química , Nanopartículas de Magnetita/química , Doença de Alzheimer/líquido cefalorraquidiano , Doença de Alzheimer/diagnóstico , Peptídeos beta-Amiloides/análise , Érbio/química , Humanos , Nanopartículas de Magnetita/ultraestrutura , Nanopartículas/química , Nanopartículas/ultraestrutura , Itérbio/química , Ítrio/química
8.
J Sep Sci ; 40(14): 2933-2940, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28556490

RESUMO

The extraction adsorbent was fabricated by immobilizing the highly specific recognition and binding of aptamer onto the surface of Fe3 O4 magnetic nanoparticles, which not only acted as recognition elements to recognize and capture the target molecule berberine from the extract of Cortex phellodendri, but also could favor the rapid separation and purification of the bound berberine by using an external magnet. The developed solid-phase extraction method in this work was useful for the selective extraction and determination of berberine in Cortex phellodendri extracts. Various conditions such as the amount of aptamer-functionalized Fe3 O4 magnetic nanoparticles, extraction time, temperature, pH value, Mg2+ concentration, elution time and solvent were optimized for the solid-phase extraction of berberine. Under optimal conditions, the purity of berberine extracted from Cortex phellodendri was as high as 98.7% compared with that of 4.85% in the extract, indicating that aptamer-functionalized Fe3 O4 magnetic nanoparticles-based solid-phase extraction method was very effective for berberine enrichment and separation from a complex herb extract. The applicability and reliability of the developed solid-phase extraction method were demonstrated by separating berberine from nine different concentrations of one Cortex phellodendri extract. The relative recoveries of the spiked solutions of all the samples were between 95.4 and 111.3%, with relative standard deviations ranging between 0.57 and 1.85%.


Assuntos
Berberina/isolamento & purificação , Medicamentos de Ervas Chinesas/química , Nanopartículas de Magnetita , Phellodendron/química , Reprodutibilidade dos Testes , Extração em Fase Sólida
9.
J Thorac Cardiovasc Surg ; 148(6): 3148-55.e1-5, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25240522

RESUMO

OBJECTIVE: Ventilator-associated pneumonia (VAP) is the most common and serious nosocomial infection that threatens patients who have undergone cardiac surgery. This article summarizes its clinical characteristics and provides theoretical evidence for prevention and treatment. METHODS: A literature search was conducted using PubMed, Embase, the Cochrane Library, and Web of Knowledge databases and by manual search. Data involving the prevalence, etiology, risk factors, or clinical outcomes were extracted for systematic review and meta-analysis. RESULTS: Eleven studies on VAP after cardiac surgery were included. When the results were merged the VAP rate was 21.27/1000 ventilator-days. The prevalence reached 6.37% of all patients and 35.2% of patients who were on mechanical ventilation for more than 48 hours. Among the isolated pathogens, Pseudomonas aeruginosa had the highest detection rate, with an average of 23.19%, followed by Staphylococcus aureus (20.15%), Haemophilus influenzae (19.53%), Acinetobacter baumannii (10.68%), Escherichia coli (10.18%), Klebsiella pneumoniae (9.52%), and Candida albicans (7.20%). Risk factors were also analyzed. We found that New York Heart Association cardiac function class IV, pulmonary hypertension, chronic obstructive pulmonary disease, peripheral vascular disease, renal disease, emergency surgery, intra-aortic balloon counterpulsation, cardiopulmonary bypass time, aortic crossclamp time, mechanical ventilation time, reintervention, and reintubation were closely related to the occurrence of VAP; there was no association with gender and diabetes mellitus. Once patients had VAP, mortality and length of stay in the intensive care unit were significantly increased. CONCLUSIONS: VAP in patients after cardiac surgery is common and has a poor prognosis. It is mainly caused by gram-negative bacteria, and could be affected by a series of factors.


Assuntos
Procedimentos Cirúrgicos Cardíacos/efeitos adversos , Infecção Hospitalar/microbiologia , Pneumonia Bacteriana/microbiologia , Pneumonia Associada à Ventilação Mecânica/microbiologia , Respiração Artificial/efeitos adversos , Procedimentos Cirúrgicos Cardíacos/mortalidade , Distribuição de Qui-Quadrado , Infecção Hospitalar/diagnóstico , Infecção Hospitalar/mortalidade , Infecção Hospitalar/prevenção & controle , Infecção Hospitalar/terapia , Humanos , Unidades de Terapia Intensiva , Tempo de Internação , Razão de Chances , Pneumonia Bacteriana/diagnóstico , Pneumonia Bacteriana/mortalidade , Pneumonia Bacteriana/prevenção & controle , Pneumonia Bacteriana/terapia , Pneumonia Associada à Ventilação Mecânica/diagnóstico , Pneumonia Associada à Ventilação Mecânica/mortalidade , Pneumonia Associada à Ventilação Mecânica/prevenção & controle , Pneumonia Associada à Ventilação Mecânica/terapia , Prevalência , Respiração Artificial/mortalidade , Medição de Risco , Fatores de Risco , Fatores de Tempo
10.
Zhonghua Yi Xue Za Zhi ; 88(47): 3325-8, 2008 Dec 23.
Artigo em Chinês | MEDLINE | ID: mdl-19257961

RESUMO

OBJECTIVE: To explore the causes of death and risk factors in patients of war wound and trauma of extremities. METHODS: This retrospective study involved 352 patients of war wound and trauma of extremities admitted to 303rd Hospital of People's Liberation Army during the period between 1968 to 2002. All the data were reviewed and the causes of death of 15 patients were analyzed by autopsy, and a computer's logistic regression model analysis was performed to approach the risk factors of death. RESULTS: Fifteen of the three hundred and fifty-two patients were died (4.3 %). The causes of death included acute renal failure (ARF) (46.7%, 7/15), lung embolism (20.0%, 3/15), clostridial myonecrosis (20.0%, 3/15) and multiple organ system failure (MOSF) (13.3%, 2/15). In the univariate analysis, the risk of death increased by shock, time admitted to hospital, amputation, time of tourniquet, associated injury of head, thoracic region, abdomen or blood vessel (P < 0. 05). In the logistic regression model analysis, shock and amputation were the two factors most strongly associated with the death of patients of war wound and trauma. (P < 0. 05). CONCLUSION: Acute renal failure (ARF) was the main cause of death of patients of war wound and trauma of extremities. Its should be helpful for minimize the mortality of patients of war wound and trauma to manage the shock in time and have a correct choice of amputation promptly.


Assuntos
Mortalidade Hospitalar , Guerra , Ferimentos e Lesões/mortalidade , Injúria Renal Aguda/mortalidade , Adolescente , Adulto , Estudos de Casos e Controles , Causas de Morte , Criança , Extremidades/lesões , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Ferimentos por Arma de Fogo/mortalidade
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