Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.040
Filtrar
1.
J Med Virol ; 92(1): 71-77, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31471921

RESUMO

Gastric cancer (GC) is one of the infection-related cancers. Helicobacter pylori and Epstein-Barr virus (EBV) were established risk factors for GC. Recently, there are several reports showing the inconsistent association between hepatitis B virus (HBV) infection and the development of GC. To explore the relationship between HBV infection and the development of GC, we designed a meta-analysis of previous epidemiological studies, a hospital-based case-control study, followed by an immunohistochemistry (IHC) assay of HBV-exposed GC samples. We found that HBV infection was associated with an increased risk of GC based on the meta-analysis. No significant association between HBV infection and GC was detected according to our hospital-based case-control study. Histological examination showed that the gastric epithelium positive for HBx demonstrated a higher nuclear-cytoplasmic ratio compared to those HBx-negative cells. HBx and HBcAg were expressed more in tumors than those in normal counterparts in HBV-exposed subjects, and PD-L1 was lower in GC tissues from HBV carriers than those in HBV clearances. In conclusion, HBV infection may contribute to a higher risk for GC based on the meta-analysis and to the morphological atypia of gastric epithelium by the histological assessment, and GC patients among HBV carriers showed lower expression of PD-L1 may lose the chance for immune checkpoint blockade therapy.

2.
Biomed J ; 42(5): 335-342, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31783994

RESUMO

BACKGROUND: Liver transplantation (LT) for recurrent hepatocellular carcinoma (HCC) following liver resection (LR) has been considered a promising strategy for improving patient's outcome. The study aimed to analyse patients from primary LR to LT for HCC and to provide additional information for decision-making in therapeutic strategies for patients with HCC. METHODS: Among 776 LTs, a retrospective analysis of patients who had undergone LT for recurrent HCC after primary LR between May 2005 and 2017 February was performed. RESULTS: During the follow-up period, the overall recurrence-free survival rates at 1, 3 and 5 years were 84.8%, 68.2% and 68.2%, and disease-specific overall-survival rates were 95.7%, 74.4% and 66.7% at 1, 3 and 5 years after LT, respectively. Beyond University of California at San Francisco (UCSF) transplantation criteria (p = 0.018, hazard ratio (HR) = 12.70), maximum tumor size ≥ 5 cm at LR (p = 0.012, HR = 7.90) and period between post-LR HCC recurrence and LT ≥ 1 year (p = 0.030, HR = 7.57) were prognostic factors of HCC recurrence after LT. Moreover, HCC recurrence after LT was the solely independent risk factor affecting overall survival of patients. CONCLUSION: Large tumor size at LR should be taken into cautious tending to HCC recurrence even after salvage LT. Importantly, LT should be considered as soon as possible preferably within 1 year whenever post-LR recurrent HCC meets transplantation criteria.

3.
Front Immunol ; 10: 2564, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31787970

RESUMO

Enterovirus and Coxsackievirus are the major viruses that cause hand, foot, and mouth disease (HFMD) outbreaks worldwide. Several studies have shown the potential of viral envelope protein 1 (VP1) on providing protective effects from viral strains of different genotypes. However, whether VP1 has the cross-protection in Enteroviruses or Coxsackievirus has not been studied in-depth. In this study, the vp1 gene of Enterovirus 71 (EV71) and Coxsackievirus B3 (CB3) was inserted into the vector pET22b (+) to form the respective expression plasmids pEVP1 or pCVP1, and then transformed into Escherichia coli strain BL21 (DE3). The recombinant EVP1 or CVP1 protein was overexpressed successfully and effectively purified to homogeneity. Then, we identified that EVP1 and CVP1 protein could generate effectively specific humoral immunity and cellular immunity in mice, what's more, we determined the cross-protection of VP1 between EV71 and CB3 in a murine model. The results showed that immunization with EVP1 could effectively induce specific IgG and secretory IgA against CVP1 and the sera from EVP1-immunized mice could neutralize CB3 with mean titers 1:440. In contrast, no measurable neutralizing antibodies to EV71 were detected in CVP1-immunized mice. Then, newborn BALB/C mice, whose mother was immunized with EVP1 or CVP1, were administered with different lethal doses of EV71 or CB3. The EVP1 immunized group showed a 90% protective efficacy for a CB3 dosage of 120 LD50, but the CVP1 immunized group showed no significantly different protective efficacy against 15 LD50 of EV71 compared with the BSA immunized group. Hence, EVP1 is a promising subunit vaccine candidate against Enterovirus 71 and Coxsackievirus B3 caused HFMD.

4.
J Agric Food Chem ; 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31789021

RESUMO

The main purpose of the present study was to investigate the browning effects of 6-gingerol (6G), one of the main functional compounds in an ethyl acetate extract of ginger (ginger ethyl acetate fraction, GEF), and its underlying mechanisms. In this study, we first discovered that GEF stimulated brown adipocyte differentiation by upregulating the expression levels of browning-specific transcription makers (UCP1, PRDM16 and PGC-1α), thereby reducing lipid metabolism transcriptional regulator (C/EBPα) expression in 3T3-L1 differentiated adipocytes. Then, 6G (47.81 ± 0.62 mg/g) was identified as one of the main functional compounds in GEF using high-performance liquid chromatography (HPLC). 6G promoted adipocyte browning, as evidenced by increases in some brown/beige fat-specific genes (PGC-1α, Cidea, Prdm16, Cited1, SIRT1, Tmem26 and Ucp1) and protein (UCP1, CEBP/ß, PGC-1α and PRDM16) expression levels. Moreover, 6G greatly improved mitochondrial respiration and energy metabolism by upregulating the expression levels of some mitochondrial biogenesis markers (Tfam, Nrf1, SIRT1 and p-AMPK/AMPK) and increasing the uncoupled oxygen consumption rate (OCR) of protons leaked in 3T3-L1 cells. Comparison of the experimental results obtained with an inhibitor (dorsomorphin) and an activator (5-aminoimidazole-4-carboxamide ribonucleotide, AICAR) suggested that the 6G-associated regulation of energy metabolism effect was mediated partly through the AMPK signaling pathway. This study provides new insight into the promotion of fat browning and lipid metabolism regulation by 6G and thus suggests that 6G likely has potential therapeutic effects on obesity.

5.
Nucleic Acids Res ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31701128

RESUMO

An integrative multi-omics database is needed urgently, because focusing only on analysis of one-dimensional data falls far short of providing an understanding of cancer. Previously, we presented DriverDB, a cancer driver gene database that applies published bioinformatics algorithms to identify driver genes/mutations. The updated DriverDBv3 database (http://ngs.ym.edu.tw/driverdb) is designed to interpret cancer omics' sophisticated information with concise data visualization. To offer diverse insights into molecular dysregulation/dysfunction events, we incorporated computational tools to define CNV and methylation drivers. Further, four new features, CNV, Methylation, Survival, and miRNA, allow users to explore the relations from two perspectives in the 'Cancer' and 'Gene' sections. The 'Survival' panel offers not only significant survival genes, but gene pairs synergistic effects determine. A fresh function, 'Survival Analysis' in 'Customized-analysis,' allows users to investigate the co-occurring events in user-defined gene(s) by mutation status or by expression in a specific patient group. Moreover, we redesigned the web interface and provided interactive figures to interpret cancer omics' sophisticated information, and also constructed a Summary panel in the 'Cancer' and 'Gene' sections to visualize the features on multi-omics levels concisely. DriverDBv3 seeks to improve the study of integrative cancer omics data by identifying driver genes and contributes to cancer biology.

6.
Int J Radiat Biol ; : 1-10, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31682784

RESUMO

Purpose: This study evaluated the DNA double strand breaks (DSBs) induced by indirect actions and its misrepairs to estimate the relative biological effectiveness (RBE) of proton beams.Materials and methods: From experimental data, DSB induction was evaluated in cells irradiated by 62 MeV proton beams in the presence of dimethylsulphoxide (DMSO) and under hypoxic conditions. The DNA damage yields for calculating the RBE were estimated using Monte Carlo Damage Simulation (MCDS) software. The repair outcomes (correct repairs, mutations and DSB conversions) were estimated using Monte Carlo Excision Repair (MCER) simulations.Results: The values for RBE of 62 MeV protons (LET = 1.051 keV/µm) for DSB induction and enzymatic DSB under aerobic condition (21% O2) was 1.02 and 0.94, respectively, as comparing to 60Co γ-rays (LET = 2.4 keV/µm). DMSO mitigated the inference of indirect action and reduced DSB induction to a greater extent when damaged by protons rather than γ-rays, resulting in a decreased RBE of 0.86. DMSO also efficiently prevented enzymatic DSB yields triggered by proton irradiation and reduced the RBE to 0.83. However, hypoxia (2% O2) produced a similar level of DSB induction with respect to the protons and γ-rays, with a comparable RBE of 1.02.Conclusions: The RBE values of proton beams estimated from DSB induction and enzymatic DSB decreased by 16% and 12%, respectively, in the presence of DMSO. Our findings indicate that the overall effects of DSB induction and enzymatic DSB could intensify the tumor killing, while alleviate normal tissue damage when indirect actions are effectively interrupted.

7.
Future Med Chem ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31718289

RESUMO

Sugar ligand molecules, such as mannose, galactose and glucose, can bind to drug delivery systems, making them targeted. These glycosylation ligands have the advantages of nontoxicity, no immunogenicity, good biocompatibility and biodegradation. They can be widely used in glycosylation-modified drug delivery systems. Herein, the targeting mechanisms, synthesis methods and targeting characteristics of glycosylation-modified drug delivery systems were reviewed.

8.
Medicine (Baltimore) ; 98(44): e17696, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689796

RESUMO

Liver resection (LR) is the standard procedure for treating colorectal cancer (CRC) hepatic metastasis; however, LR associated with a high recurrence incidence. This study aimed to determine an optimal post-LR adjuvant chemotherapeutic strategy to improve overall long-term patient outcomes. A retrospective study of 490 patients who had undergone curative LR for CRC hepatic metastasis was performed. Patients who underwent post-LR adjuvant chemotherapy demonstrated high overall survival (OS) rates (hazard ratio [HR] = 0.58, P = .002) but not high recurrence-free survival (RFS) rates (HR = 1.02, P = .885). Moreover, OS was significantly longer in patients who underwent 5-fluorouracil + leucovorin (5-FU/LV; HR = 0.63, P = .039), oxaliplatin-based chemotherapy (HR = 0.45, P < .001), or irinotecan-based chemotherapy with bevacizumab (HR = 0.64, P = .040) than in those who did not. Among patients with carcinoembryonic antigen (CEA) levels of <5 ng/mL at 1 month after LR, significant differences were noted only in those who underwent 5-FU/LV (HR = 0.58, P = .035) and oxaliplatin-based chemotherapy (HR = 0.38, P < .001). In conclusion, perioperative CEA levels are crucial in prognosis and treatment of patients with CRC hepatic metastasis after LR. Additionally, certain regimens of adjuvant chemotherapy alongside post-LR CEA levels may provide beneficial results.

9.
Nanotechnology ; 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31711047

RESUMO

The dissolution and diffusion of polysulfides leading to inferior cycling performance are still the main bottleneck hindering commercialization of Li-S battery, although they have received many restrictive policies in recent years. Herein, a new strategy that lithium polyacrylate (LiPAA) was introduced to clad on multiwalled carbon nanotube/sulfur (MWNT/S) composites as the interface layer for MWNT/S/LiPAA cathode, was proposed to not only suppress polysulfides migration through physical encapsulation and chemical adsorption but also facilite Li+ diffusion during the charge-discharge process. Attributing to these functions of LiPAA, the MWNT/S/LiPAA exhibited the rate capability and cycling performance superior to MWNT/S and MWNT/S/PAA. Moreover, thanking to the introduction of LiPAA, the MWNT/S/LiPAA were endowed with robust mechanical property, which was qualified as flexible cathode to assemble flexible Li-S battery with stable output under deformation condition. This work would open up a promising way to suppress polysulfides migration for high-performance flexible Li-S battery.

10.
Inflamm Res ; 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31712853

RESUMO

BACKGROUND: Sepsis, a life-threatening systemic syndrome related to inflammatory response, usually accompanied by major organ dysfunctions. The aim of the present study was to elucidate the role by which Shengmai injection (SMI) acts to septic cardiomyopathy. METHODS: Initially, the induced mice with septic cardiomyopathy were treated with SMI or normal saline (NS) with oe-caspase-3, and HL-1 cells were treated with oe-Beclin-1 and oe-caspase-3 and then cultured with lipopolysaccharide (LPS). Subsequently, we measured the cardiac troponin I (cTnI) level, and expression of mitochondrial autophagy protein (parkin and pink1) and myocardial cell autophagy-related proteins (LC3-II and LC3-I). Additionally, we identified the cleavage of Beclin-1 by caspase-3 and detected the changes of mitochondrial membrane potential, level of reactive oxygen species (ROS), and apoptosis of myocardial cells in myocardial tissues of mice. RESULTS: It has been demonstrated that SMI contributed to the increase of myocardial mitochondrial autophagy, reduction of cTnI level, and elevation of mitochondrial membrane potential in septic cardiomyopathy mice. Both in vitro and in vivo experiments showed that caspase-3 promoted cleavage of Beclin-1 and release of ROS, whereas repressed lipopolysaccharide (LPS)-induced mitochondrial autophagy. Furthermore, the facilitation of myocardial mitochondrial autophagy and protection of myocardial mitochondria by SMI through inhibition of cleavage Beclin-1 by caspase-3 in septic cardiomyopathy mice were also proved by in vivo experiments. CONCLUSION: Taken together, SMI could protect myocardial mitochondria by promoting myocardial mitochondrial autophagy in septic cardiomyopathy via inhibition of cleavage of Beclin-1 by caspase-3. Our study demonstrates that SMI could represent a novel target for treatment of septic cardiomyopathy.

11.
Appl Opt ; 58(29): 8092-8100, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31674368

RESUMO

Laser-plane-based vision reconstruction is an important approach for 3D profile measurement and optical inspection. This study develops a 3D reconstruction method to measure the object with a flexible method. The active vision reconstruction includes an external camera and a 3D reference with an internal camera and laser projector. The 3D calibration object, the external camera, and the 3D reference constitute a closed loop. The internal camera, the 3D calibration object, and the 3D reference compose the other closed loop. The intrinsic invariants of the 3D reference with an internal camera and laser projector are determined by the two loops above. The benefit of the method is to cancel the position constraint for the active vision system with the cubic LED reference and the monocular external camera. Furthermore, as the external camera captures only the images of the 3D reference, the reconstruction system of the projector and the camera is flexible to observe the occlusion location on the measured object. The methods, including the calibration model and reconstruction model of the measurement system, are verified by experiments to demonstrate the potential applications.

12.
Spectrochim Acta A Mol Biomol Spectrosc ; 227: 117666, 2019 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-31670045

RESUMO

Herein, a facile and quick strategy to detect thiourea was conducted based on the reversion of fluorescence quenching of nitrogen doped carbon dots (NCDs) by Hg2+. The NCDs with good water solubility and 17% of quantum yield was synthesized by one-step hydrothermal method, using ammonium citrate and dextrin as carbon source and nitrogen source, respectively. The fluorescence of NCDs was obviously quenched by Hg2+ and can be recovered, due to stronger interaction between thiourea and Hg2+. There was a good linear relationship between the recovered fluorescence and the concentration of thiourea within range of 0.90-10.0 µM and the detection limit for thiourea detection was 0.15 µM. The as-prepared NCDs can be used for determination of thiourea in tap water, lake water and rice flour products, and the spike recoveries were between 91.6 and 108%.

13.
Chem Commun (Camb) ; 55(93): 14074-14077, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31696869

RESUMO

We discovered that the function of cytochrome C can be modulated by DNA nanoribbons. Meanwhile, the interplay between the DNA nanoribbons and the native cytochrome C and the possible mechanisms are also discussed.


Assuntos
Citocromos c/metabolismo , DNA/metabolismo , Nanoestruturas/química , Peroxidases/metabolismo , Animais , Catálise , Citocromos c/química , DNA/química , Guaiacol/química , Cavalos , Oxirredução , Peroxidases/química , Ligação Proteica
14.
Int J Biol Macromol ; 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31756474

RESUMO

As a natural and renewable biological macromolecule, dextran not only has excellent biodegradability, but also has good biocompatibility. Dextran and its derivatives are functional polymers for the construction of targeted drug delivery systems. Herein, the application of dextran as prodrug and nanoparticle/nanogel/microsphere/micelle carrier for targeting drug delivery system was summarized. It is clarified that dextran is an important biomaterial with application value.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31779190

RESUMO

As a "starting zone" and "amplifier" of global climate change, the Qinghai-Tibet Plateau is very responsive to climate change. The global temperature rise has led directly to an acceleration of glacial melting in the plateau and various glacier avalanche disasters have frequently occurred. The landslide caused by glacier avalanches will damage the surrounding environment, causing secondary disasters and a disaster chain effect. Take the disaster chain of the Yarlung Zangbo River at Milin County in Tibet on 17 and 29 October 2018 as an example; a formation mechanical model was proposed. The evolution mechanism for the chain of events is as follows: glacial melt → loose moraine deposit → migration along the steep erosion groove resulting in glacier clastic deposition then debris flow → formation of the dam plug to block the river → the dammed lake. This sequence of events is of great significance for understanding the developmental trends for future avalanches, landslides, and river blocking dam disasters, and for disaster prevention planning and mitigation in the Qinghai-Tibet Plateau.

17.
Chem Commun (Camb) ; 55(95): 14387-14390, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31723950

RESUMO

We designed a tandem stimuli-responsive assembly based on a guanidinium-modified calix[5]arene (GC5A-6C) and eosin Y modified hyaluronic acid (EY-HA), which showed hyaluronidase-triggered disassembly and ATP-activated release of EY. Both hyaluronidase and ATP are tumor biomarkers, and therefore, the present system shows potential in precision delivery with respect to tumor phototheranostics.

18.
BMC Med Genet ; 20(1): 186, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31752724

RESUMO

BACKGROUND: We have performed this study to evaluate the association between H19 rs217727 polymorphism and the risk of cancer. METHODS: An odds ratio (OR) with a 95% confidence interval (CI) was applied to determine a potential association. RESULTS: A total of 17 case-control publications were selected. This meta-analysis showed that H19 rs217727 has a significant increased association with cancer risk in allelic, homozygous, heterozygote, dominant and recessive models (T vs C: OR = 1.16, 95% CI = 1.06-1.27, I2 = 75.7; TT vs CC: OR = 1.29, 95% CI = 1.06-1.56, I2 = 71.6; CT vs CC: OR = 1.15, 95% CI = 1.01-1.31, I2 = 75.4; CT + TT vs CC: OR = 1.20, 95% CI = 1.05-1.36, I2 = 76.5; TT vs CT + CC: OR = 1.22, 95% CI = 1.02-1.45, I2 = 70.6;). In the subgroup analysis of smoking status, both smokers and nonsmokers showed an increase in cancer risk in allelic, homozygous, dominant and heterozygote models. CONCLUSION: This meta-analysis revealed H19 rs217727 may influence cancer susceptibility.

19.
Medicine (Baltimore) ; 98(45): e17816, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31702635

RESUMO

Massive blood transfusion (MBT) increased mortality and morbidity after cardiac surgery. However, a mid-term follow-up study on repair surgery of acute type A aortic dissection (AAAD) with MBT was lacking. This study aimed to assess the impact of perioperative MBT on late outcomes of surgical repair for AAAD.There were 3209 adult patients firstly received repair surgery for AAAD between 2005 and 2013, were identified using Taiwan National Health Insurance Research Database. Primary interest variable was MBT, defined as transfused red blood cell (RBC) ≥10 units.The outcomes contained in-hospital mortality, surgical-related complications, all-cause mortality, respiratory failure, and chronic kidney disease (CKD) during follow-up period. Higher in-hospital mortality (37.7% vs 11.6%; odds ratio, 4.00; 95% confidence interval [CI], 3.30-4.85), all-cause mortality (26.1% vs 13.0%; hazard ratio [HR], 1.66; 95% CI, 1.36-2.04), and perioperative complications were noted in the MBT group. A subdistribution hazard model revealed higher cumulative incidence of CKD (13.9% vs 6.5%; HR, 1.95; 95% CI, 1.47-2.60) and respiratory failure (7.1% vs 2.7%; HR, 2.34; 95% CI, 1.52-3.61) for the MBT cohort. A dose-dependent relationship between amount of transfused RBC (classified as tertiles) and cumulative incidence of all-cause mortality, incident CKD, and respiratory failure was found (P of trend test <.001).Patients with MBT had worse late outcomes following surgical repair of AAAD. The cumulative incidence of all-cause mortality, incident CKD, and respiratory failure increased with the amount of transfused RBC in a dose-dependent manner.


Assuntos
Aneurisma Dissecante/cirurgia , Perda Sanguínea Cirúrgica/prevenção & controle , Transfusão de Sangue/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Aneurisma Dissecante/mortalidade , Perda Sanguínea Cirúrgica/mortalidade , Feminino , Mortalidade Hospitalar , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Taiwan , Resultado do Tratamento , Adulto Jovem
20.
Int J Biochem Cell Biol ; 118: 105644, 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31712163

RESUMO

Although the early diagnosis of prostate cancer (PCa) enhances life expectancy with a 5-year survival rate of 100 %, metastasized-PCa is the fundamental reason for death by PCa, hence requires an advanced and target-directed treatment strategy. Metastasis is considered to be initiated with the epithelial-mesenchymal transition (EMT) event in which tumor cells change their epithelial characteristics into mesenchymal form and exacerbates the cancer progression. Herein, we investigated the effect and mechanism of resveratrol function in PCa cell proliferation and migration and reported that TNF-receptor associated factor 6 (TRAF6), an unconventional E3 ligase, is a key mediator of resveratrol function to inhibit PCa cell growth and proliferation and targeted for lysosomal degradation by resveratrol. MTT and cell counting demonstrated that resveratrol inhibited the viability and proliferation in DU145 and PC3 cells. Resveratrol (50 µM) mediated the degradation of TRAF6 which in turn facilitated repression of the NF-κB pathway. Also, wound healing and transwell migration assays and level of EMT-related proteins showed that resveratrol used TRAF6, at least in part to inhibit cell migration. Overexpression of TRAF6 augmented EMT in PCa by upregulating the expression of transcription factor SLUG. Moreover, TRAF6 overexpression was closely associated with EMT process through the NF-κB pathway. Our exploration exhibited that resveratrol may inhibit EMT through the TRAF6/NF-κB/SLUG axis. Altogether, this study represents that TRAF6 acts as an intermediary of resveratrol action to suppress PCa cell proliferation and migration, and concerns future attention to obtain as a therapeutic target for the treatment of PCa.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA