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1.
Mol Med Rep ; 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31485619

RESUMO

The main purpose of the present study was to recognize the integrative genomics analysis of hub genes and their relationship with prognosis and signaling pathways in esophageal squamous cell carcinoma (ESCC). The mRNA gene expression profile data of GSE38129 were downloaded from the Gene Expression Omnibus database, which included 30 ESCC and 30 normal tissue samples. The differentially expressed genes (DEGs) between ESCC and normal samples were identified using the GEO2R tool. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to identify the functions and related pathways of the genes. The protein­protein interaction (PPI) network of these DEGs was constructed with the Search Tool for the Retrieval of Interacting Genes and visualized with a molecular complex detection plug­in via Cytoscape. The top five important modules were selected from the PPI network. A total of 928 DEGs, including ephrin­A1 (EFNA1), collagen type IV α1 (COL4A1),  C­X­C chemokine receptor 2 (CXCR2), adrenoreceptor ß2 (ADRB2), P2RY14, BUB1B, cyclin A2 (CCNA2), checkpoint kinase 1 (CHEK1), TTK, pituitary tumor transforming gene 1 (PTTG1) and COL5A1, including 498 upregulated genes, were mainly enriched in the 'cell cycle', 'DNA replication' and 'mitotic nuclear division', whereas 430 downregulated genes were enriched in 'oxidation­reduction process', 'xenobiotic metabolic process' and 'cell­cell adhesion'. The KEGG analysis revealed that 'ECM­receptor interaction', 'cell cycle' and 'p53 signaling pathway' were the most relevant pathways. According to the degree of connectivity and adjusted P­value, eight core genes were selected, among which those with the highest correlation were CHEK1, BUB1B, PTTG1, COL4A1 and CXCR2. Gene Expression Profiling Interactive Analysis in The Cancer Genome Atlas database for overall survival (OS) was applied among these genes and revealed that EFNA1 and COL4A1 were significantly associated with a short OS in 182 patients. Immunohistochemical results revealed that the expression of PTTG1 in esophageal carcinoma tissues was higher than that in normal tissues. Therefore, these genes may serve as crucial predictors for the prognosis of ESCC.

2.
Extremophiles ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31489482

RESUMO

16S rRNA gene profiling is a powerful method for characterizing microbial communities; however, no universal primer pair can target all bacteria and archaea, resulting in different primer pairs which may impact the diversity profile obtained. Here, we evaluated three pairs of high-throughput sequencing primers for characterizing archaeal communities from deep-sea sediments and permafrost soils. The results show that primer pair Arch519/Arch915 (V4-V5 regions) produced the highest alpha diversity estimates, followed by Arch349f/Arch806r (V3-V4 regions) and A751f/AU1204r (V5-V7 regions) in both sample types. The archaeal taxonomic compositions and the relative abundance estimates of archaeal communities are influenced by the primer pairs. Beta diversity of the archaeal community detected by the three primer pairs reveals that primer pairs Arch349f/Arch806r and Arch519f/Arch915r are biased toward detection of Halobacteriales, Methanobacteriales and MBG-E/Hydrothermarchaeota, whereas the primer pairs Arch519f/Arch915r and A751f/UA1204r are biased to detect MBG-B/Lokiarchaeota, and the primers pairs Arch349f/Arch806r and A751f/UA1204r are biased to detect Methanomicrobiales and Methanosarcinales. The data suggest that the alpha and beta diversities of archaeal communities as well as the community compositions are influenced by the primer pair choice. This finding provides researchers with valuable experimental insight for selection of appropriate archaeal primer pairs to characterize archaeal communities.

3.
Int J Biol Macromol ; 141: 14-20, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31473317

RESUMO

The effect of derivatization on the antioxidant activity of bitter gourd polysaccharide was investigated. Bitter gourd polysaccharide (P) was prepared with hot water. Carboxymethylated bitter gourd polysaccharide (CM-P) and acetylated bitter gourd polysaccharide (AcP) were successfully prepared, which were identified by IR and NMR spectra. On this basis, the antioxidant activities of three polysaccharides were tested, including hydroxyl radical scavenging capacity, DPPH radical scavenging capacity, and anti-lipid peroxidation capacity. The results showed that different chemical modifications had different enhancement on the antioxidant activities of bitter gourd polysaccharide. So, bitter gourd polysaccharide could be used to develop potential antioxidants.

4.
Neurosci Lett ; : 134487, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31513835

RESUMO

Fear extinction is considered as a new learning process that is valid to model features of post-traumatic stress disorder (PTSD). The neuropeptide apelin, such as apelin-13, apelin-17 and apelin-36, are endogenous ligands of the G-protein coupled receptor APJ. Apelin and its receptor APJ are widely distributed in the central nervous system. Accumulating evidence suggests the critical role of apelin-13 in modulation of learning and memory, however, its specific role in fear extinction remains unclear. In the present study, we investigated the effect of apelin-13 administration on contextual fear extinction in rats. The behavioral procedure included four sessions: habitation, conditioning, extinction training and extinction recall. Rats received intracerebroventricular infusion of apelin-13 (3 or 6 µg) 0.5 h prior to the extinction training. Percentage of freezing was utilized to assess the conditioned fear response. Results showed that apelin-13, with the dose of 6 but not 3 µg, significantly decreased freezing response during both extinction training and extinction recall test sessions. Furthermore, apelin-13 did not affect the levels of baseline freezing, locomotor activity and anxiety. The results suggest that apelin-13 dose-dependently enhances contextual fear extinction, and may function as a novel target for treatment of PTSD.

5.
Neurotoxicology ; 2019 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-31509761

RESUMO

Acrylamide (AA) is a common food contaminant known to detrimentally affect the nervous system. Homeostasis of the nervous system is dependent on glial cells, namely astrocytes and microglia, which actively participate in neuronal survival signaling pathways. Although the differential responses of monocultured astrocytes compared to co-cultured astrocytes and microglia to AA exposure have been investigated, the global effects and potential molecular mechanism involved in AA-induced neurotoxicity remain unknown. In this study, the impacts of AA on primary monocultured astrocytes and co-cultured astrocytes with microglia were determined using Orbitrap-based proteomic analysis. The results showed that AA exposure mainly caused disruption of multicellular metabolic processes, biological regulation, and cell development. Furthermore, oxidative stress-related pathways and immune responses were the main regulatory functions influenced by AA-induced neurotoxicity. Additionally, Nrf2 and other downstream proteins in the oxidative stress-related pathway were up-regulated. There were significant differences between the protein changes in the monocultured astrocytes and co-cultured astrocytes with microglia, indicating that AA affected cell-cell communication between astrocytes and microglia. Overall, these findings illustrate the global effects of AA-induced functionality and pathway alteration and their involvement in the development of neurological deficits in primary glial cell cultures. These findings may provide new insights for the development of a pathway approach suitable for the risk assessment of AA.

6.
J Med Virol ; 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31471921

RESUMO

Gastric cancer (GC) is one of the infection-related cancers. Helicobacter pylori and Epstein-Barr virus (EBV) were established risk factors for GC. Recently, there are several reports showing the inconsistent association between hepatitis B virus (HBV) infection and the development of GC. To explore the relationship between HBV infection and the development of GC, we designed a meta-analysis of previous epidemiological studies, a hospital-based case-control study, followed by an immunohistochemistry (IHC) assay of HBV-exposed GC samples. We found that HBV infection was associated with an increased risk of GC based on the meta-analysis. No significant association between HBV infection and GC was detected according to our hospital-based case-control study. Histological examination showed that the gastric epithelium positive for HBx demonstrated a higher nuclear-cytoplasmic ratio compared to those HBx-negative cells. HBx and HBcAg were expressed more in tumors than those in normal counterparts in HBV-exposed subjects, and PD-L1 was lower in GC tissues from HBV carriers than those in HBV clearances. In conclusion, HBV infection may contribute to a higher risk for GC based on the meta-analysis and to the morphological atypia of gastric epithelium by the histological assessment, and GC patients among HBV carriers showed lower expression of PD-L1 may lose the chance for immune checkpoint blockade therapy.

7.
J Am Soc Nephrol ; 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31506292

RESUMO

BACKGROUND: Apoptosis signal-regulating kinase 1 (ASK1) activation in glomerular and tubular cells resulting from oxidative stress may drive kidney disease progression. Findings in animal models identified selonsertib, a selective ASK1 inhibitor, as a potential therapeutic agent. METHODS: In a phase 2 trial evaluating selonsertib's safety and efficacy in adults with type 2 diabetes and treatment-refractory moderate-to-advanced diabetic kidney disease, we randomly assigned 333 adults in a 1:1:1:1 allocation to selonsertib (oral daily doses of 2, 6, or 18 mg) or placebo. Primary outcome was change from baseline eGFR at 48 weeks. RESULTS: Selonsertib appeared safe, with no dose-dependent adverse effects over 48 weeks. Although mean eGFR for selonsertib and placebo groups did not differ significantly at 48 weeks, acute effects related to inhibition of creatinine secretion by selonsertib confounded eGFR differences at 48 weeks. Because of this unanticipated effect, we used piecewise linear regression, finding two dose-dependent effects: an acute and more pronounced eGFR decline from 0 to 4 weeks (creatinine secretion effect) and an attenuated eGFR decline between 4 and 48 weeks (therapeutic effect) with higher doses of selonsertib. A post hoc analysis (excluding data for 20 patients from two sites with Good Clinical Practice compliance-related issues) found that between 4 and 48 weeks, rate of eGFR decline was reduced 71% for the 18-mg group relative to placebo (difference 3.11±1.53 ml/min per 1.73 m2 annualized over 1 year; 95% confidence interval, 0.10-6.13; nominal P=0.043). Effects on urine albumin-to-creatinine ratio did not differ between selonsertib and placebo. CONCLUSIONS: Although the trial did not meet its primary endpoint, exploratory post hoc analyses suggest that selonsertib may slow diabetic kidney disease progression.

9.
Clin Ther ; 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31447131

RESUMO

PURPOSE: The Heart Protection Study 2-Treatment of HDL to Reduce the Incidence of Vascular Events (HPS2-THRIVE) trial of patients at high risk of vascular disease found that adding extended-release niacin-laropiprant to intensive statin-based LDL-lowering therapy had no benefit on cardiovascular outcomes. However, the trial also identified previously unrecognized serious adverse effects (including new-onset diabetes, bleeding, and infection). Our objective was to explore the safety profile of niacin-laropiprant and examine whether any patients were at lower (or higher) risk of its adverse effects. METHODS: HPS2-THRIVE was a randomized, double-blind trial of niacin-laropiprant (2000/40 mg/d) versus placebo among 25,673 patients at high risk of vascular disease. Information on all serious adverse events was collected during a median of 3.9 years of study treatment. Effects of niacin-laropiprant on new-onset diabetes, disturbances of diabetes control, bleeding, infection, and gastrointestinal upset were estimated by (1) time after randomization, (2) severity, (3) baseline characteristics, (4) baseline risk of the adverse event of interest, and (5) risk of major vascular event. FINDINGS: The hazard ratio (HR) for new-onset diabetes with niacin/laropiprant was 1.32 (95% CI, 1.16-1.51; P < .001), which corresponded to an absolute excess of 4 people (95% CI, 2-6) developing diabetes per 1000 person-years in the study population as a whole. Among the 8299 participants with diabetes at baseline, the HR for serious disturbances in diabetes control was 1.56 (95% CI, 1.35-1.80), corresponding to an absolute excess of 12 (95% CI, 8-16) per 1000 person-years. The HR was 1.38 (95% CI, 1.17-1.63; P < .001) for serious bleeding, corresponding to an absolute excess of 2 (95% CI, 1-3) per 1000 person-years and 1.22 (95% CI, 1.11-1.34; P < .001) for serious infection, corresponding to an absolute excess of 4 (95% CI, 2-6) per 1000 person-years. The excess risks of these serious adverse events were larger in the first year after starting niacin-laropiprant therapy than in later years (except for the excess of infection, which did not appear to attenuate with time), and the risks of nonfatal and fatal events were similarly increased. The absolute excesses of each of these adverse effects were similar regardless of the baseline risk of the outcome. IMPLICATIONS: Practitioners or patients considering the use of niacin (in addition to, or instead of, a statin) despite the lack of evidence of cardiovascular benefits (at least when added to effective statin therapy) should take account of the significant risks of these serious adverse effects when making such decisions. ClinicalTrials.gov identifier: NCT00461630.

10.
Nat Commun ; 10(1): 3824, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31444352

RESUMO

The interfacial sites of supported metal catalysts are often critical in determining their performance. Single-atom catalysts (SACs), with every atom contacted to the support, can maximize the number of interfacial sites. However, it is still an open question whether the single-atom sites possess similar catalytic properties to those of the interfacial sites of nanocatalysts. Herein, we report an active-site dependent catalytic performance on supported gold single atoms and nanoparticles (NPs), where CO oxidation on the single-atom sites is dramatically promoted by the presence of H2O whereas on NPs' interfacial sites the promoting effect is much weaker. The remarkable H2O promoting effect makes the Au SAC two orders of magnitude more active than the commercial three-way catalyst. Theoretical studies reveal that the dramatic promoting effect of water on SACs originates from their unique local atomic structure and electronic properties that facilitate an efficient reaction channel of CO + OH.

11.
Talanta ; 205: 120135, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31450416

RESUMO

C-reactive protein (CRP) is one of the important indicators of inflammatory response. It plays as an essential role in diseases such as myocardial infarction and atherosclerosis. A simple and effective label-free electrochemiluminescent (ECL) immunosensor for quantitative detection of CRP is reported. The indium tin oxide coated glass was prefunctionalized with the composite of titania nanotubes and platinum nanowires as the sensing matrix. Then the CRP antibody was immobilized on it, hosted the sensing function owing to its specific binding with CRP caused by immune affinity. The characteristics of the fabricated sensor were investigated by cyclic voltammetry, electrochemical impedance spectroscopy and electron microscopes. The sensor has the advantages of high sensitivity, good selectivity, good reproducibility and low cost. Under the optimal experimental conditions, the response of ECL signal on obtained sensor has a good linear regression toward the logarithm of CRP quantity within the range from 0.05 ng to 6.25 ng with a detection limit of 0.011 ng. The immunosensor is small, convenient to use, and provides a feasible way for domestically rapid detection of CRP. It has a promising future in the precaution of cardiovascular and other diseases.

12.
ACS Appl Mater Interfaces ; 11(34): 31477-31483, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31385689

RESUMO

Surface coating and lattice doping are widely used to enhance the interfacial and structural stabilities of Li1.2Ni0.13Co0.13Mn0.54O2 (LNCM). In this paper, KF is used to modify LNCM for the first time. A Li+/K+ exchange in the Li slabs is realized via a high-temperature treatment. Consequently, subsurface K+ gradient doping and surface K1-xLixF gradient coating are obtained simultaneously on LNCM. Such an Li+/K+ exchange mechanism and double-gradient modification are clarified by X-ray diffraction, energy-dispersive spectrometry line scans, and high-resolution transmission electron microscopy analyses. As a result, the optimal 0.5 wt % KF-modified LNCM material shows markedly alleviated voltage degradation (0.0031 V@1 cycle), improved cycling stability (88%@100 cycles@0.5 C), and rate capability (108 mA h g-1@10 C), revealing large application potential in high-energy materials.

13.
Neurol Sci ; 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31392640

RESUMO

The main purpose of this study was to systematically evaluate the accuracy of neuromelanin-sensitive magnetic resonance imaging (NM-MRI) in Parkinson's disease (PD) diagnosis using a meta-analysis method. In PubMed, Web of Science, Embase, and Google Scholar, the literatures were searched for the diagnostic value of neuromelanin-sensitive magnetic resonance imaging in PD. The literatures were screened in the light of Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Data analysis was processed by Stata 12.0 software to obtain meta-analysis, heterogeneity analysis, and publication bias. Meta-analysis results showed by using NM-MRI observed substantia nigra pars compacta (SNpc) on PD, the pooled diagnostic sensitivity and specificity were 0.82 (95% CI, 0.74-0.87) and 0.82 (95% CI, 0.73-0.89), respectively. And the pooled positive likelihood ratio (PLR) and negative likelihood ratio (NLR) were 4.58 (95% CI, 3.08-6.82) and 0.22 (95% CI, 0.16-0.31), respectively. Moreover, subgroup analysis according to the measurement criteria of SNpc showed the SNpc volume should be used as good a marker for diagnosing PD. Finally, Fagan test demonstrated that when PLR was equal to 5, the posterior probability is significantly enhanced to 53%, compared with prior probability (20%). As for NLR (0.22), the prior probability is 20%, while the posterior probability remarkably dropped to 5%. In conclusion, SNpc signal detected by NM-MRI exhibited high sensitivity and specificity for diagnosis of PD, which was a high-performance imaging diagnostic method for PD. We recommend NM-MRI imaging technology to be widely used in Parkinson's diagnosis.

14.
Neurol Sci ; 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31463680

RESUMO

The above article was published online with incorrect author name. The right spelling should be Xiangming Wang instead of Xiangmin Wang. The correct name is presented here. The original article has been corrected.

15.
Environ Pollut ; 254(Pt A): 112959, 2019 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-31377327

RESUMO

Humic acids (HAs) have been shown to dominate the photodegradation of steroid estrogens in natural waters. Nevertheless, how the photosensitizing ability of HAs relates to their structural and optical characteristics remains largely unknown. In this study, 17α-ethynylestradiol (EE2) was selected as a model compound to study to what extent easily-measurable characteristics of HAs might be used to predict their photosensitization potency. HAs were extracted from sediments of two different sources, and then subjected to structural and optical properties characterization using elemental analyzer, UV-vis spectroscopy and fluorescence spectroscopy. Photochemical experiments show that the HAs from the two sources can effectively meditate EE2 photodegradation. Although with drastically different structural and optical properties, the photosensitizing ability of these HAs towards EE2 can be well described by simple linear regressions using a spectroscopic index, the spectral slope ratio (SR). This optical indicator is correlated with various physicochemical properties of HAs, including the molecular weight, lignin content, charge-transfer interaction potential, photobleaching extent and sources. No universal prediction model could be established for predicting EE2 photodegradation kinetics on the basis of SR, but in specific waters SR could be a powerful indictor for predicting the EE2 photodegradation sensitized by HAs.

16.
Brain Behav ; : e01385, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31429527

RESUMO

BACKGROUND: Synaptic plasticity is the basis of memory formation. The pathological manifestations of abnormal glucose metabolism in the nervous system of sporadic Alzheimer's disease (SAD) may affect synaptic plasticity, thus causing memory damage. As a traditional Chinese medicine compound preparation, the mechanism by which Shenzhiling (SZL) oral liquid can alleviate the cognitive impairment of SAD by improving synaptic plasticity remains unclear. OBJECTIVE: This article mainly discusses whether SZL can exert a protective synaptic effect as mediated by glutamate receptors and glycogen synthesis kinase 3ß (GSK3ß); further, it discusses whether SZL can improve cognitive function in SAD. METHODS: C57BL/6 mice were used as a SAD model after injection with streptozotocin (STZ) into the bilateral lateral ventricles; mice of the same background were injected with artificial cerebrospinal fluid into bilateral ventricles and were used as a control group. After 3 months of exposure to the intervention, the step-down test was carried out. Western blot was used to detect the levels of NMDAR2B, p-NMDAR2B, mGlu5, GSK3ß, and p-GSK3ß in the hippocampus of mice. Immunohistochemical analysis was used to observe the number of GSK3ß-positive cells in the CA1 region of mouse hippocampus. RESULTS: The memory retention ability of mice was significantly improved after 3 months of SZL treatment, and the expression levels of NMDAR2B, mGlu5, and GSK3ß were significantly changed. CONCLUSION: Shenzhiling provides a potential for the treatment for SAD with traditional Chinese medicine.

17.
Macromol Rapid Commun ; 40(17): e1900101, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31364800

RESUMO

Polyhedral oligomeric silsesquioxane (POSS)-hybrid polymers have been successfully employed as functional inorganic-organic hybrid materials for various applications due to their well-determined structures. The past 6 years has witnessed growing interest in the rational design and synthetic approaches for POSS-hybrid polymers, driven by the adoption of controlled living radial polymerization and click chemistry. This review addresses developments in the precise manipulation of POSS building blocks via atom transfer radical polymerization, reversible addition-fragmentation chain transfer, and click chemistry. Not only are the structures of POSS-hybrid polymers tunable in terms of chemical composition, molecular weight, and polydispersity, but they are also controllable in sequential and hierarchical chain topology. Finally, some representative cutting-edge applications of POSS-hybrid polymers, including biomedical and energy-related materials, fabrication of nanostructures, and functional surface coating materials, are highlighted.

18.
Nat Commun ; 10(1): 3906, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31467269

RESUMO

Mycobacterium tuberculosis infection remains a major threat to human health worldwide. Drug treatments against tuberculosis (TB) induce expression of several mycobacterial proteins, including IniA, but its structure and function remain poorly understood. Here, we report the structures of Mycobacterium smegmatis IniA in both the nucleotide-free and GTP-bound states. The structures reveal that IniA folds as a bacterial dynamin-like protein (BDLP) with a canonical GTPase domain followed by two helix-bundles (HBs), named Neck and Trunk. The distal end of its Trunk domain exists as a lipid-interacting (LI) loop, which binds to negatively charged lipids for membrane attachment. IniA does not form detectable nucleotide-dependent dimers in solution. However, lipid tethering indicates nucleotide-independent association of IniA on the membrane. IniA also deforms membranes and exhibits GTP-hydrolyzing dependent membrane fission. These results confirm the membrane remodeling activity of BDLP and suggest that IniA mediates TB drug-resistance through fission activity to maintain plasma membrane integrity.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31385166

RESUMO

Intestinal epithelial cells (IECs) not only have an absorption function but also act as a physical barrier between the body and the intestinal bacterial flora. Damage to IECs leads to the breakdown of this barrier and has negative effects on animal health. Intestinal epithelial damage is frequently associated with long-term acute stress, such as increased temperature and new stress management models. The intestinal epithelial damage caused by environmental stress has been linked to oxidative stress. Until now, the effects of intestinal epithelial antioxidant activity from feed additives and treatments could be tested in ducks only in vivo because of the lack of in vitro cell culture systems. In this study, we describe our protocol for the easy isolation and culture of IECs from the small intestine of duck embryos. Immunofluorescence was used for the cytological identification of IECs. In addition, IEC marker genes (IAP and CDH1) could also be detected in cultured cells. And cell status assessments were performed, and cell proliferation viability was analyzed by CCK-8 assay. Furthermore, we constructed an oxidative stress model to be used to research the oxidative stress response mechanism, and drugs acting on the cell signal transduction pathway. In conclusion, we have developed an effective and rapid protocol for obtaining duck primary IECs and constructed an oxidative stress model. These IECs exhibit features consistent with epithelial cells and could be used to explore the physiological mechanisms of oxidative stress ex vivo.

20.
Artif Cells Nanomed Biotechnol ; 47(1): 3368-3373, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31387395

RESUMO

Diabetic nephropathy (DN) is one of the major diabetic complications that lead to end-stage renal failure. Angiopoietin-like protein-4 (ANGPTL-4) has been reported to be dysregulated in diabetes mellitus and diabetic complications. However, the role of ANGPTL-4 in glomerular mesangial cells (MCs) during DN remains unclear. In the present study, we evaluated the role of ANGPTL-4 in MCs in response to high glucose (HG) condition and the potential mechanism. The results proved that ANGPTL-4 expression is significantly increased in HG-stimulated MCs. Knockdown of ANGPTL-4 suppressed HG-induced cell proliferation of MCs. The production of pro-inflammatory cytokines including TNF-α, IL-1ß, IL-6 were decreased in ANGPTL-4 knocked down MCs. Inhibition of ANGPTL-4 markedly suppressed the expressions of extracellular matrix (ECM) proteins, collagen IV (Col IV) and fibronectin (FN), in HG-stimulated MCs. Furthermore, ANGPTL-4 knockdown inhibited the HG-induced activation of NF-κB signaling pathway in MCs. Collectively, knockdown of ANGPTL-4 suppressed HG-induced cell proliferation, inflammatory response, and ECM accumulation inhibiting NF-κB signaling pathway in MCs. These findings suggested that ANGPTL-4 might be a therapeutic target for the prevention and treatment of DN.

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