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1.
Int J Biol Macromol ; 133: 564-574, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31004640

RESUMO

Dendrobium devonianum has been used as herbal medicines and nutraceutical products since ancient time in China. However, its chemical composition and pharmacological mechanisms are not fully known. In present studies, by chemical purification and characteristic identification, we discovered a novel polysaccharide from D. devonianum, which was designated as DvP-1 with molecular weights of 9.52 × 104 Da. DvP-1 is a homogeneous heteropolysaccharide consisting of D-mannose and d-glucose in the molar ration of 10.11: 1. The main glycosidic linkages were ß-1, 4-Manp, which were substituted with acetyl groups at the O-2, O-3 and/or O-6 positions. DvP-1 was found to directly stimulate the activation of macrophages in vitro, as evidenced by inducing morphologic change, thereby promoting the production of cytokines TNF-α, IL-6 and NO, and enhancing the pinocytic activity of macrophages. By establishing a zebrafish model, we also found that DvP-1 could alleviate vinorelbine-induced decrease of macrophages in vivo. Further findings indicated that DvP-1 activated macrophages through several toll-like receptors (TLRs), but mainly through TLR4. DvP-1 served as a TLR4 agonist and induced ERK, JNK, p38, and IκB-α phosphorylation, suggesting the activation of MAPK and NFκB signaling pathways downstream of TLR4. These findings could help us further understand the immunomodulating effects of D. devonianum in Chinese medicines or health foods for immunocompromised persons. They also show the medicinal value of DvP-1 for the treatment of cancer and infectious diseases caused by TLR4 dysfunction.


Assuntos
Dendrobium/química , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Polissacarídeos/farmacologia , Receptor 4 Toll-Like/agonistas , Animais , Proliferação de Células/efeitos dos fármacos , Citocinas/biossíntese , Macrófagos/citologia , Camundongos , Monossacarídeos/análise , Polissacarídeos/química , Células RAW 264.7 , Baço/imunologia , Vinorelbina/farmacologia , Peixe-Zebra
2.
Cancer Lett ; 420: 195-207, 2018 04 28.
Artigo em Inglês | MEDLINE | ID: mdl-29425683

RESUMO

Uncontrolled excessive activation of Hedgehog (Hh) signaling pathway is linked to a number of human malignant tumorigenesis. To obtain valuable Hh pathway inhibitors from natural product, in present study, a pair of novel epimers, Cynanbungeigenin C (CBC) and D (CBD) from the plant Cynanchum bungei Decne were chemically characterized by multiple spectroscopic data and chemical derivatization, and evaluated for their inhibition on Hh pathway. Mechanistically, CBC and CBD block Hh pathway signaling not through targeting Smo and Sufu, but at the level of Gli. In addition, both eipmers significantly suppress Hh pathway-dependent Ptch+/-; p53-/- medulloblastoma in vitro and in vivo. Furthermore, both CBC and CBD inhibited two Smo mutants induced Hh pathway activation, which suggested that they are potential compounds for the treatment of medulloblastoma with primary or acquired resistance to current Smo inhibitors. These results highlight the potential of CBC and CBD as effective lead compounds in the treatment of medulloblastoma and other Hh-dependent malignancy.


Assuntos
Neoplasias Cerebelares/tratamento farmacológico , Cynanchum/química , Meduloblastoma/tratamento farmacológico , Fitosteróis/administração & dosagem , Fitosteróis/isolamento & purificação , Transdução de Sinais/efeitos dos fármacos , Animais , Neoplasias Cerebelares/metabolismo , Células HEK293 , Proteínas Hedgehog/metabolismo , Humanos , Meduloblastoma/metabolismo , Camundongos , Células NIH 3T3 , Fitosteróis/química , Fitosteróis/farmacologia , Extratos Vegetais/análise , Ensaios Antitumorais Modelo de Xenoenxerto , Proteína GLI1 em Dedos de Zinco/metabolismo
3.
Front Microbiol ; 8: 1251, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28725220

RESUMO

An endophytic fungus, Mycosphaerella nawae ZJLQ129, was isolated from the leaves of the traditional Chinese medicine Smilax china. From the fermentation broth and mycelium, a dibenzofurane compound (-)mycousnine (1) was isolated. Chemical modification of it to the amide derivative (-)mycousnine enamine (2), which is new to science, was found to have high and selective immunosuppressive activity: similar to cyclosporin A, (-)mycousnine enamine (2) selectively inhibited T cell proliferation, suppressed the expression of the surface activation antigens CD25 and CD69 and the formation and expression of the cytokines interleukin-2 as well as interferon γ in activated T cells, but did not show any effect on the proliferation of B cells and cancer cells (PANC-1 and A549) and the activation of macrophages. Furthermore, the cytotoxicity of (-)mycousnine enamine was lower than that of cyclosporin A, and its therapeutic index (TC50/EC50) was 4,463.5, which is five-fold higher than that of cyclosporin A. We conclude that (-)mycousnine enamine (2), the semi-synthestic product prepared from the native product (-)mycousnine (1) of the endophyte M. nawae is a novel effective immunosuppressant showing low toxicity and high selectivity.

4.
Nat Prod Commun ; 11(6): 747-8, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27534107

RESUMO

Two new 13, 14/14, 15-disecopregnane-type skeleton C21 steroidal aglycones, neocynapanogenin G (1) and neocynapanogenin H (2), were isolated from the hydrolyzed extract of the CHCl3 soluble extract of the roots of Cynanchun paniculatum. Their structures were determined on the basis of chemical evidence and extensive spectroscopic methods, including 1D and 2D NMR spectroscopy. Compound 1 displayed signifidant inhibition of the Hedgehog signaling pathway in vitro.


Assuntos
Cynanchum/química , Medicamentos de Ervas Chinesas/química , Iridoides/química , Raízes de Plantas/química , Esteroides/química , Animais , Linhagem Celular , Ouriços-Cacheiros/genética , Ouriços-Cacheiros/metabolismo , Humanos , Estrutura Molecular , Transdução de Sinais/efeitos dos fármacos
5.
Eur J Pharmacol ; 789: 370-384, 2016 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-27448502

RESUMO

Stephanthraniline A (STA), a C21 steroid isolated from Stephanotis mucronata (Blanco) Merr., was previously shown to inhibit T cells activation and proliferation in vitro and in vivo. The purpose of this study was to further evaluate the in vivo immunosuppressive activity of STA and to elucidate its potential mechanisms. The results showed that pretreatment with STA significantly attenuated concanavalin A (Con A)-induced hepatitis and reduced CD4(+) T cells activation and aggregation in hepatic tissue in mice. STA directly suppressed the activation and proliferation of Con A-induced CD4(+) T cells, and inhibited NFAT, NFκB and MAPK signaling cascades in activated CD4(+) T cells in vitro. Moreover, it was proved that STA inhibited T cells activation and proliferation through proximal T cell-receptor (TCR) signaling- and Ca(2+) signaling-independent way. The molecular docking studies predicted that STA could tight bind to PKCθ via five hydrogen. The further findings indicated STA directly inhibited PKCθ kinase activity, and its phosphorylation in activated CD4(+) T cells in vitro. Collectively, the present study indicated that STA could protect against CD4(+) T cell-mediated immunological hepatitis in mice through PKCθ and its downstream NFAT, NFκB and MAPK signaling cascades. These results highlight the potential of STA as an effective leading compound for use in the treatment of CD4(+) T cell-mediated inflammatory and autoimmune diseases.


Assuntos
Linfócitos T CD4-Positivos/efeitos dos fármacos , Linfócitos T CD4-Positivos/imunologia , Diterpenos/farmacologia , Hepatite/tratamento farmacológico , Hepatite/imunologia , Isoenzimas/antagonistas & inibidores , Ativação Linfocitária/efeitos dos fármacos , Proteína Quinase C/antagonistas & inibidores , Animais , Sinalização do Cálcio/efeitos dos fármacos , Domínio Catalítico , Agregação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Diterpenos/metabolismo , Diterpenos/uso terapêutico , Feminino , Hepatite/metabolismo , Hepatite/patologia , Isoenzimas/química , Isoenzimas/metabolismo , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Camundongos , Simulação de Acoplamento Molecular , NF-kappa B/metabolismo , Fatores de Transcrição NFATC/metabolismo , Fosforilação/efeitos dos fármacos , Proteína Quinase C/química , Proteína Quinase C/metabolismo , Proteína Quinase C-theta , Proteínas Quinases/metabolismo
6.
Chem Biodivers ; 13(11): 1484-1492, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27449706

RESUMO

Stemucronatoside K (SMK) and its aglycone stephanthraniline A (STA) are the most representative of a series of novel C21 steriodal compounds that we have previously isolated from Asclepiadaceae plants. The objectives of this study were to investigate the antitumor activity of SMK and STA, and clarify the effect of the sugar chain at the C(3) position. Our results showed that both SMK and STA decreased the growth of HT-29 cells in a dose- and time-dependent manner. Meanwhile, STA showed much stronger inhibitory effect than SMK. Treatment of HT-29 cells with STA increased the apoptotic cell numbers and the protein expression of cleaved caspase 3 and cleaved-PARP. G1 phase cell cycle arrest and decreased expression of cyclin D1 and cyclin-dependent kinases 4 were also observed after STA treatment. Furthermore, STA reduced the mRNA levels of four Hedgehog pathway components (GLI1, GLI2, GLI3, and PTCH1) and suppressed Shh-induced Hedgehog pathway activation in a concentration-dependent manner. These results indicated that SMK and STA could inhibit the growth of HT-29 cells by inducing apoptosis, cell cycle arrest, and hedgehog pathway inhibition. The loss of sugar chain at C(3) position could enhance SMK's activity. This study is beneficial to understand the use of natural C21 steroids as antitumor lead compounds.


Assuntos
Apoptose/efeitos dos fármacos , Carboidratos/química , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Saponinas/farmacologia , Transdução de Sinais/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Células HT29 , Proteínas Hedgehog/genética , Proteínas Hedgehog/metabolismo , Humanos , Conformação Molecular , Saponinas/química , Relação Estrutura-Atividade
7.
Nat Prod Commun ; 11(12): 1797-1800, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30508336

RESUMO

Two new 8, 14-seco skeleton C(21) steroidal aglycones, cynanbungeigenin A (1) and cynanbungeigenin B (2), were isolated from the hydrolyzed extract of the EtOAc soluble extract of the roots of Cynanchum bungei. Their structures were determined on the basis of chemical evidence and extensive spectroscopic methods, including 1D and 2D NMR spectroscopy.


Assuntos
Cynanchum/química , Pregnanos/isolamento & purificação , Animais , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Proteínas Hedgehog/antagonistas & inibidores , Camundongos , Células NIH 3T3 , Extratos Vegetais/química , Raízes de Plantas/química , Pregnanos/química , Pregnanos/farmacologia , Espectroscopia de Prótons por Ressonância Magnética
8.
Nat Prod Commun ; 9(2): 155-6, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24689277

RESUMO

Two novel steroidal aglycones, together with four known ones, were isolated from the hydrolysis extract of the CHCl3 soluble extract of the stems of Marsdenia tenacissima. Their structures were determined on the basis of chemical evidence and extensive spectroscopic methods, including 1D and 2D NMR spectroscopy. These compounds displayed inhibition of the Hedgehog signaling pathway in vitro.


Assuntos
Proteínas Hedgehog/antagonistas & inibidores , Marsdenia/química , Transdução de Sinais/efeitos dos fármacos , Esteroides/isolamento & purificação , Extratos Vegetais/análise , Caules de Planta/química , Esteroides/farmacologia
9.
J Ethnopharmacol ; 153(3): 831-9, 2014 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-24690776

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The larvae of Musca domestica (Diptera: Muscidae) have been used traditionally for malnutritional stagnation, decubital necrosis, osteomyelitis, ecthyma and lip scald and also to treat coma and gastric cancer in the traditional Chinese medicine. Its in vitro antitumor activity and immunomodulatory effect in naïve mice in relation to the traditional uses were also reported. However, the in vivo antitumor effect of this insect and its mechanism of action have not yet been well studied. The objectives of this study were to evaluate the in vivo antitumor potential of the peptide fraction from Musca domestica larvae (MDPF) and to elucidate its immunological mechanisms. MATERIALS AND METHODS: The mice inoculated with sarcoma S180 cells were orally administered with MDPF at three doses for 10 days. The effects of MDPF on the growth of mouse S180 sarcoma, splenocyte proliferation, the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), production and mRNA expression of cytokines from splenocytes, and serum antigen-specific antibody levels in tumor-bearing mice were measured. RESULTS: MDPF could significantly not only inhibit the growth of mouse transplanted S180 sarcoma, but also promote splenocytes proliferation, NK cell and CTL activity from splenocytes, and enhance serum antigen-specific IgG, IgG2a and IgG2b antibody levels in S180-bearing mice. MDPF also significantly promoted the production of IFN-γ and up-regulated the mRNA expression levels of IFN-γ and Th1 transcription factors T-bet and STAT-4 in splenocytes from the S180-bearing mice. However, Th2 cytokine IL-10 and transcription factors GATA-3 and STAT-6 were not significantly changed both at transcriptional and protein levels following MDPF treatment. CONCLUSIONS: MDPF significantly inhibit the growth of transplantable tumor in mice and its in vivo antitumor activity might be achieved by switching-on of Th1-based protective cell-mediated immunity. MDPF could act as antitumor agent with immunomodulatory activity.


Assuntos
Antineoplásicos , Produtos Biológicos , Moscas Domésticas , Fatores Imunológicos , Peptídeos , Sarcoma 180/tratamento farmacológico , Animais , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Produtos Biológicos/farmacologia , Produtos Biológicos/uso terapêutico , Linhagem Celular Tumoral , Feminino , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Fatores Imunológicos/farmacologia , Fatores Imunológicos/uso terapêutico , Interferon gama/genética , Interferon gama/imunologia , Interleucina-10/genética , Células Matadoras Naturais/efeitos dos fármacos , Células Matadoras Naturais/imunologia , Larva/química , Masculino , Camundongos Endogâmicos ICR , Peptídeos/farmacologia , Peptídeos/uso terapêutico , RNA Mensageiro/metabolismo , Sarcoma 180/imunologia , Baço/citologia , Baço/imunologia , Linfócitos T Citotóxicos/efeitos dos fármacos , Linfócitos T Citotóxicos/imunologia , Fatores de Transcrição/genética
11.
Chem Biodivers ; 8(6): 1121-31, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21674784

RESUMO

The total crude polysaccharides (CADPs), isolated from the roots of Angelica dahurica by H(2) O extraction, EtOH precipitation, and dialysis, and the four fractions ADP1, ADP2, ADP3, and ADP4, obtained by gel filtration of the CADPs, were analyzed to characterize their composition and evaluated for their antioxidant activity using different in vitro tests such as the malondialdehyde (MDA)-production, the ferrous ion (Fe(2+) )-chelating, and the HO(.) radical-scavenging assays. The predominant neutral monosaccharides in the four fractions were identified as arabinose, galactose, and glucose, while the composition and ratio of the monosaccharides were different between the fractions. The CADPs and its fractions were found to significantly inhibit lipid peroxidation, chelate Fe(2+) , and scavenge HO(.) radicals, indicating that these polysaccharides possessed antioxidant activity. Among the four fractions, ADP4 exhibited the strongest antioxidant activity, which was stronger than that of the control antioxidant vitamin E (Vit E). Taken together, the chemical composition of these polysaccharides might affect their antioxidant activity, and ADP4 could be explored as a source of potential novel natural antioxidants for food and pharmaceutical purposes.


Assuntos
Angelica/química , Depuradores de Radicais Livres/química , Polissacarídeos/química , Cromatografia em Gel , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/farmacologia , Quelantes de Ferro/química , Quelantes de Ferro/isolamento & purificação , Quelantes de Ferro/farmacologia , Malondialdeído/metabolismo , Raízes de Plantas/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia
12.
Chem Biodivers ; 6(8): 1243-53, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19697343

RESUMO

3beta,6beta-dihydroxyolean-12-en-27-oic acid (1) is a pentacyclic triterpenoid isolated from the rhizomes of Astilbe chinensis. To evaluate the in vivo antitumor potential and to elucidate its immunological mechanisms, effect of 1 on the growth of mouse-transplantable tumors, and the immune response in naive and tumor-bearing mice were investigated. The mice inoculated with mouse tumor cell lines were orally treated with 1 at the doses of 40, 60, and 80 mg/kg for 10 days. The effects of 1 on the growth of mouse-transplantable S180 sarcoma and H22 hepatoma, splenocyte proliferation, cytotoxic T lymphocyte (CTL) activity, natural killer (NK) cell activity, and production of interleukin-2 (IL-2) from splenocytes in S180-bearing mice were measured. Furthermore, the effect of 1 on 2,4-dinitrofluorobenzene (DNFB)-induced delayed-type hypersensitivity (DTH) reactions and the sheep red blood cell (SRBC)-induced antibody response in naive mice were also studied. Compound 1 could not only significantly inhibit the growth of mouse transplantable S180 sarcoma and H22 hepatoma, increase splenocytes proliferation, CTL and NK cell activity, and the level of IL-2 secreted by splenocytes in tumor-bearing mice, but also remarkably promote the DTH reaction and enhance anti-SRBC antibody titers in naive mice. These results suggested that 1 could improve both cellular and humoral immune response, and could act as antitumor agent with immunomodulatory activity.


Assuntos
Antineoplásicos Fitogênicos/uso terapêutico , Fatores Imunológicos/uso terapêutico , Ácido Oleanólico/análogos & derivados , Animais , Feminino , Hipersensibilidade Tardia/tratamento farmacológico , Interleucina-2/metabolismo , Masculino , Camundongos , Ácido Oleanólico/uso terapêutico , Sarcoma 180/tratamento farmacológico
13.
Chem Biodivers ; 6(6): 916-23, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19551733

RESUMO

Stemucronatoside L (SML), isolated from Stephanotis mucronata, could suppress the activation of T cells in vitro. However, the mechanisms responsible for its immunosuppressive activity remain poorly understood. The purpose of this study was to investigate whether SML could suppress Th1/Th2 immune responses and to characterize the cellular mechanisms involved. Effects of SML on T-lymphocyte subsets and the production of Th1 cytokines IL-2 and IFN-gamma, and Th2 cytokines IL-4 and IL-10 from ConA-stimulated mice splenocytes were detected by flow-cytometric analysis and ELISA method, respectively. Furthermore, effects of SML on mRNA expression level of Th1/Th2 cytokines and transcription factors T-bet and GATA-3 were evaluated by RT-PCR analysis. SML not only significantly decreased the percentage of CD4(+) T cells and the CD4(+)/CD8(+) ratio, but reduced the production of Th1/Th2 cytokines in a concentration-dependent manner. The mRNA expression levels of Th1/Th2 cytokines and transcription factors (T-bet and GATA-3) were also suppressed by SML. These results suggested that SML could simultaneously inhibit Th1/Th2 immune responses by suppressing gene expression of Th1/Th2 cytokines and transcription factors.


Assuntos
Apocynaceae/química , Glicosídeos/química , Raízes de Plantas/química , Pregnanos/química , Saponinas/química , Células Th1/efeitos dos fármacos , Células Th2/efeitos dos fármacos , Animais , Concanavalina A/farmacologia , Feminino , Fator de Transcrição GATA3/genética , Fator de Transcrição GATA3/metabolismo , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Interferon gama/genética , Interferon gama/metabolismo , Interleucina-10/genética , Interleucina-10/metabolismo , Interleucina-2/genética , Interleucina-2/metabolismo , Interleucina-4/genética , Interleucina-4/metabolismo , Camundongos , Camundongos Endogâmicos ICR , Pregnanos/isolamento & purificação , Pregnanos/farmacologia , Saponinas/isolamento & purificação , Saponinas/farmacologia , Proteínas com Domínio T/genética , Proteínas com Domínio T/metabolismo , Células Th1/imunologia , Células Th2/imunologia
14.
J Ethnopharmacol ; 125(2): 310-7, 2009 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-19559777

RESUMO

AIM OF THE STUDY: The roots of Actinidia eriantha Benth (Actinidiaceae) have been used for cancers in the Chinese folk medicine. The present study aimed at evaluating the antitumor potentials of the polysaccharides from the roots of Actinidia eriantha and elucidating their immunological mechanisms by determining the effects on the growth of tumor transplanted in mice and the immune response in tumor-bearing mice. MATERIALS AND METHODS: The total polysaccharide AEP and fours purified polysaccharides AEPA, AEPB, AEPC and AEPD were isolated and purified from the roots of Actinidia eriantha by hot water extraction, ethanol precipitation, dialysis and gel filtration. Their effects on the growth of mouse transplantable tumor, splenocyte proliferation, the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTL), production of cytokines from splenocytes, and serum antigen-specific antibody levels in tumor-bearing mice were measured. RESULTS: AEP and four purified polysaccharides could not only significantly inhibit the growth of mouse transplantable tumor, but also remarkably promote splenocytes proliferation, NK cell and CTL activity, IL-2 and IFN-gamma production from splenocytes, and serum antigen-specific antibody levels in tumor-bearing mice. CONCLUSIONS: The antitumor activity of AEP and four purified polysaccharides might be achieved by improving immune response, and the composition of the monosaccharides, uronic acid contents and molecular weight could affect their antitumor and immunomodulatory activity.


Assuntos
Actinidia/química , Antineoplásicos Fitogênicos/uso terapêutico , Citocinas/metabolismo , Fatores Imunológicos/uso terapêutico , Linfócitos/metabolismo , Neoplasias Experimentais/tratamento farmacológico , Polissacarídeos/uso terapêutico , Animais , Anticorpos/sangue , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Fatores Imunológicos/isolamento & purificação , Fatores Imunológicos/farmacologia , Interferon gama/metabolismo , Interleucina-2/metabolismo , Células Matadoras Naturais/metabolismo , Masculino , Camundongos , Transplante de Neoplasias , Fitoterapia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Raízes de Plantas , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Sarcoma 180 , Baço/citologia , Linfócitos T Citotóxicos/metabolismo
15.
Zhongguo Zhong Yao Za Zhi ; 33(20): 2356-8, 2008 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-19157127

RESUMO

OBJECTIVE: To study the chemical constituents from n-BuOH fraction of the roots of Stephanotis mucronata. METHOD: The compounds were separated by chromatographic methods. A combination of UV, MS, and NMR spectroscopic methods was applied to identify structure of these compounds. RESULT: Four oleane saponins were isolated and identified as sitakisoside VII (1), sitakisoside VI (2), sitakisoside II (3), and sitakisoside I (4). CONCLUSION: These four compounds were obtained for the first time from this plant.


Assuntos
Apocynaceae/química , Medicamentos de Ervas Chinesas/química , Raízes de Plantas/química , Saponinas/química , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Estrutura Molecular
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