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1.
Biol Pharm Bull ; 46(2): 187-193, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36724947

RESUMO

Endoplasmic reticulum (ER) dysfunction is characterized by ER stress, which can be triggered by sepsis. Recent studies have reported that lessening ER stress is a promising therapeutic approach to improving the outcome of sepsis. Genipin is derived from gardenia fruit, which is a traditional Chinese medicinal herb for anti-inflammation. Here, mice were treated with genipin (2.5 mg/kg) intravenously to assess its biological effects and underlying mechanism against polymicrobial sepsis. Furthermore, the present study focused on detecting the levels of ER stress-related proteins, including protein kinase R-like ER kinase (PERK), glucose-regulated protein of 78 kDa (GRP78), phosphorylated-eukaryotic initiation factor 2α (p-eIF2α), and CCAAT/enhancer binding protein (C/EBP) homologous protein (CHOP). The results demonstrated that genipin significantly decreased the serum concentrations of tumor necrosis factor-α and interleukin-6, alleviated histopathological damage to the lungs, livers and spleens, and even improved the survival rates of septic mice. Moreover, sepsis significantly upregulated the protein expression levels of splenic GRP78, PERK, p-eIF2α and CHOP, but their levels were significantly suppressed by genipin. Furthermore, genipin also significantly downregulated cleaved caspase-3 expression levels and reduced sepsis-induced splenocyte apoptosis. In conclusion, genipin potentially improved the survival rate of sepsis and attenuated sepsis-induced organ injury and an excessive inflammatory response in mice. The effects of genipin against sepsis were potentially associated with decreased splenocyte apoptosis via the attenuation of sepsis-induced ER stress to further inhibit ER stress-induced apoptosis.


Assuntos
Chaperona BiP do Retículo Endoplasmático , Sepse , Camundongos , Animais , Baço/metabolismo , Apoptose , Estresse do Retículo Endoplasmático , Sepse/complicações , Sepse/tratamento farmacológico , Fator de Transcrição CHOP/metabolismo
3.
Org Lett ; 25(2): 364-368, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36625525

RESUMO

Herein, host-guest complexation between pagoda[n]arenes (n = 4, P4; n = 5, P5) and tropylium cation (G) was investigated in detail. It was found that both P4 and P5 showed surprisingly strong binding affinities toward the tropylium cation with association constants of more than 107 M-1 for the 1:1 host-guest complexes. The theoretical calculations showed different host-guest complexion ways for complexes G@P4 and G@P5 and the strong π···π interactions and multiple C-H···π interactions play a very important role in the formation of these stable complexes, respectively. Moreover, the switchable processes of guest binding and release in the complexes can be effectively controlled by redox stimuli, and they can be also visible by the color and fluorescence changes.

4.
Int J Mol Sci ; 24(2)2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36674776

RESUMO

Growth-regulating factor (GRF) is a kind of transcription factor unique to plants, playing an important role in the flowering regulation, growth, and development of plants. Melastoma dodecandrum is an important member of Melastomataceae, with ornamental, medicinal, and edible benefits. The identification of the GRF gene family in M. dodecandrum can help to improve their character of flavor and continuous flowering. The members of the GRF gene family were identified from the M. dodecandrum genome, and their bioinformatics, selective pressure, and expression patterns were analyzed. The results showed that there were 20 GRF genes in M. dodecandrum. Phylogenetic analysis showed that the 71 GRF genes from M. dodecandrum, Arabidopsis thaliana, Camellia sinensis, and Oryza sativa can be divided into three clades and six subclades. The 20 GRF genes of M. dodecandrum were distributed in twelve chromosomes and one contig. Furthermore, the gene structure and motif analysis showed that the intron and motif within each clade were very similar, but there were great differences among different clades. The promoter contained cis-acting elements related to hormone induction, stress, and growth and development. Different transcriptomic expression of MdGRFs indicated that MdGRFs may be involved in regulating the growth and development of M. dodecandrum. The results laid a foundation for further study on the function and molecular mechanism of the M. dodecandrum GRF gene family.


Assuntos
Melastomataceae , Melastomataceae/química , Filogenia , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Família Multigênica , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo
5.
J Hazard Mater ; 446: 130672, 2023 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-36580778

RESUMO

Microplastics might affect the nitrogen (N)-use efficiency, crop production, and reactive N losses in agricultural system. However, it remains unclear whether the effects are dependent on crop cultivar. Here, a pot experiment was conducted to evaluate the effects of a typical polyethylene (PE) microplastics addition on grain yield and amino acid content, N-use efficiency, ammonia (NH3) volatilization and nitrous oxide (N2O) emission, and properties of paddy soil planted with common rice Nangeng 5055 (NG) and hybrid rice Jiafengyou 6 (JFY). The results showed that PE addition significantly reduced the grain yield and total grain amino acid content of hybrid rice by 23% and 1.7%, respectively. In addition, PE addition significantly decreased the N agronomic and recovery efficiencies of hybrid rice by 30% and 27%, respectively. For paddy soil in which hybrid rice was grown, PE addition significantly increased NH3 volatilization by 72%, but exerted no influence on N2O emission. Interestingly, the N2O emission from NG+PE treatment was 15% significantly lower than that from NG treatment, which was associated with decreased gene copies of nirK (by 50%) and nirS (by 84%) in NG+PE treatment. Generally, no significant change in soil properties was found as result of microplastics addition regardless of the cultivar. In conclusion, the impacts of microplastics on rice production and quality, N-use efficiency and nitrogenous gas losses from paddy soil are cultivar-dependent.


Assuntos
Oryza , Solo , Solo/química , Oryza/metabolismo , Microplásticos/análise , Plásticos/análise , Gases/análise , Agricultura , Nitrogênio/química , Amônia/metabolismo , Grão Comestível/metabolismo , Óxido Nitroso , Polietileno/análise , Fertilizantes/análise
6.
Neural Regen Res ; 18(6): 1277-1285, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-36453412

RESUMO

Dysfunction of neuronal nitric oxide synthase contributes to neurotoxicity, which triggers cell death in various neuropathological diseases, including epilepsy. Studies have shown that inhibition of neuronal nitric oxide synthase activity increases the epilepsy threshold, that is, has an anticonvulsant effect. However, the exact role and potential mechanism of neuronal nitric oxide synthase in seizures are still unclear. In this study, we performed RNA sequencing, functional enrichment analysis, and weighted gene coexpression network analysis of the hippocampus of tremor rats, a rat model of genetic epilepsy. We found damaged hippocampal mitochondria and abnormal succinate dehydrogenase level and Na+-K+-ATPase activity. In addition, we used a pilocarpine-induced N2a cell model to mimic epileptic injury. After application of neuronal nitric oxide synthase inhibitor 7-nitroindazole, changes in malondialdehyde, lactate dehydrogenase and superoxide dismutase, which are associated with oxidative stress, were reversed, and the increase in reactive oxygen species level was reversed by 7-nitroindazole or reactive oxygen species inhibitor N-acetylcysteine. Application of 7-nitroindazole or N-acetylcysteine downregulated the expression of caspase-3 and cytochrome c and reversed the apoptosis of epileptic cells. Furthermore, 7-nitroindazole or N-acetylcysteine downregulated the abnormally high expression of NLRP3, gasdermin-D, interleukin-1ß and interleukin-18. This indicated that 7-nitroindazole and N-acetylcysteine each reversed epileptic cell death. Taken together, our findings suggest that the neuronal nitric oxide synthase/reactive oxygen species pathway is involved in pyroptosis of epileptic cells, and inhibiting neuronal nitric oxide synthase activity or its induced oxidative stress may play a neuroprotective role in epilepsy.

7.
Am J Orthod Dentofacial Orthop ; 163(2): 173-180, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36476369

RESUMO

INTRODUCTION: This study aimed to evaluate the orthodontic effect and efficiency of substituting third molars for missing first or second permanent molars systematically. METHODS: Forty-six patients (69 third molars total) with missing permanent molars replaced by third molars were selected. The angulation, crown-to-root ratio, and periodontal condition of the third molars before and after treatment were compared. The American Board of Orthodontics Objective Grading System was used to evaluate the alignment and occlusion of third molars after treatment. The duration of orthodontic treatment and third molar replacement therapy were also recorded. RESULTS: The average orthodontic treatment time was 33.9 ± 5.6 months, and the average angulation change of third molars during treatment was 49.8 ± 29.8°. The average height of mesial alveolar bone increased by 4.8 ± 0.5 mm in patients whose third molars were mesially inclined or horizontally impacted. The root length of adult patients decreased by 0.72 ± 0.02 mm on average, and the average gingival recession was 0.10 mm, both of which were not statistically significant. The average score for each third molar evaluated by the American Board of Orthodontics Objective Grading System was 1.8 ± 0.5 points. CONCLUSIONS: If the indications and timing of treatment were well-controlled, third molars would be excellent substitutes for missing first or second permanent molars through the orthodontic method.


Assuntos
Retração Gengival , Doenças Periodontais , Dente Impactado , Adulto , Humanos , Dente Serotino , Dente Molar , Oclusão Dentária , Dente Impactado/diagnóstico por imagem , Dente Impactado/terapia , Mandíbula
8.
Front Behav Neurosci ; 16: 1066725, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36570704

RESUMO

Background: Inadequate sleep during pregnancy negatively affects the neural development of offspring. Previous studies have focused on the continuous sleep deprivation (CSD) paradigm, but the sleep pattern during late pregnancy is usually fragmented. Objective: To compare the effects of CSD and fragmented sleep deprivation (FSD) in late pregnancy on emotion, cognition, and expression of synaptic plasticity-related proteins in offspring mice. Methods: Pregnant CD-1 mice were either subjected to 3/6 h of CSD/FSD during gestation days 15-21, while those in the control group were left untreated. After delivery, the offspring were divided into five groups, i.e., control (CON), short or long CSD (CSD3h, CSD6h), and short or long FSD (FSD3h, FSD6h). When the offspring were 2 months old, the anxiety-like behavior level was tested using the open field (OF) and elevated plus maze (EPM) test, and spatial learning and memory were evaluated using the Morris water maze (MWM) test. The expression of hippocampal of brain-derived neurotrophic factor (Bdnf) and synaptotagmin-1 (Syt-1) was determined using RT-PCR and western blotting. Results: The CSD6h, FSD3h, and FSD6h had longer latency, fewer center times in the OF test, less open arms time and fewer numbers of entries in the open arms of the EPM, longer learning distance swam and lower memory percentage of distance swam in the target quadrant in the MWM test, and decreased BDNF and increased Syt-1 mRNA and protein levels in the hippocampus. Compared to the CSD6h, the FSD3h and FSD6h had longer distance swam, a lower percentage of distance swam in the target quadrant, decreased BDNF, and increased Syt-1 mRNA and protein levels in the hippocampus. Conclusion: The results suggested that maternal sleep deprivation during late pregnancy impairs emotion and cognition in offspring, and FSD worsened the cognitive performance to a higher extent than CSD. The observed cognitive impairment could be associated with the expression of altered hippocampal of Bdnf and Syt-1 genes.

9.
Front Aging Neurosci ; 14: 1021237, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36479357

RESUMO

Introduction: Research suggests that prenatal inflammatory exposure could accelerate age-related cognitive decline that may be resulted from neuroinflammation and synaptic dysfunction during aging. Environmental enrichment (EE) may mitigate the cognitive and synaptic deficits. Neurite growth-promoting factor 2 (NGPF2) and postsynaptic density protein 95 (PSD-95) play critical roles in neuroinflammation and synaptic function, respectively. Methods: We examined whether this adversity and EE exposure can cause alterations in Ngpf2 and Psd-95 expression. In this study, CD-1 mice received intraperitoneal injection of lipopolysaccharide (50 µg/kg) or normal saline from gestational days 15-17. After weaning, half of the male offspring under each treatment were exposed to EE. The Morris water maze was used to assess spatial learning and memory at 3 and 15 months of age, whereas quantitative real-time polymerase chain reaction and Western blotting were used to measure hippocampal mRNA and protein levels of NGPF2 and PSD-95, respectively. Meanwhile, serum levels of IL-6, IL-1ß, and TNF-α were determined by enzyme-linked immunosorbent assay. Results: The results showed that aged mice exhibited poor spatial learning and memory ability, elevated NGPF2 mRNA and protein levels, and decreased PSD-95 mRNA and protein levels relative to their young counterparts during natural aging. Embryonic inflammatory exposure accelerated age-related changes in spatial cognition, and in Ngpf2 and Psd-95 expression. Additionally, the levels of Ngpf2 and Psd-95 products were significantly positively and negatively correlated with cognitive dysfunction, respectively, particularly in prenatal inflammation-exposed aged mice. Changes in serum levels of IL-6, IL-1ß, and TNF-α reflective of systemic inflammation and their correlation with cognitive decline during accelerated aging were similar to those of hippocampal NGPF2. EE exposure could partially restore the accelerated decline in age-related cognitive function and in Psd-95 expression, especially in aged mice. Discussion: Overall, the aggravated cognitive disabilities in aged mice may be related to the alterations in Ngpf2 and Psd-95 expression and in systemic state of inflammation due to prenatal inflammatory exposure, and long-term EE exposure may ameliorate this cognitive impairment by upregulating Psd-95 expression.

10.
Obes Res Clin Pract ; 2022 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-36543613

RESUMO

A longitudinal approach with Latent Growth Curve Modeling (LGCM) was adopted to explore the trajectories of appetitive traits corresponding to BMI in Chinese adolescents. Within a large sample of adolescents (N = 2566, 45.9% boys) aged from 11 to 17 years (M = 13.80, SD = 1.56) at the baseline survey, our results indicated that appetitive traits of emotional overeating, food fussiness, and hunger increased significantly over time while enjoyment of food decreased over time. Slowness in eating and satiety responsiveness significantly increased in girls, while emotional undereating significantly decreased in boys. Moreover, the growth parameters of emotional undereating and satiety responsiveness were significantly and negatively related to BMI in girls. Our findings evidence that certain appetitive traits could change over time in adolescence and these changes relate to weight status. Gender differences are suggested in the design of future intervention and treatment of overweight/obesity in Chinese adolescents.

11.
Artigo em Inglês | MEDLINE | ID: mdl-36547832

RESUMO

The effects of interactions between the toxic and essential metal mixtures on cognitive function are poorly understood. This study aims to identify the joint association of arsenic (As), cadmium (Cd), and lead (Pb) with cognitive function in older adults and the moderating role of selenium (Se), zinc (Zn), and copper (Cu) in this association. This study included 1000 community-dwelling older adults. Cognitive function was assessed by the Mini-Mental State Examination (MMSE). Blood concentrations of As, Cd, Pb, Se, Zn, and Cu were measured using inductively coupled plasma mass spectrometry. Linear regression and Bayesian kernel machine regression (BKMR) models were applied to assess the individual and joint associations of As, Cd, and Pb with cognitive function and to examine whether Se, Zn, and Cu (individually and as a mixture) modified these associations. In the adjusted single-metal models, both Cd (ß = - 0.37, 95% CI: - 0.73 to - 0.01) and Pb (ß = - 0.44, 95% CI: - 0.86 to - 0.02) were associated with MMSE scores, while Se (ß = 0.71, 95% CI: 0.30 to 1.13) exhibited a positive relationship with MMSE scores. Univariate exposure-response functions from BKMR models showed similar results. Moreover, the toxic metal mixture (As, Cd, and Pb) exhibited a significant negative association with MMSE scores in a dose-response pattern, with Pb being the greatest contributor within the mixture. The negative association of Pb alone or the toxic metal mixture with MMSE scores became weaker at higher concentrations of Se within its normal range, especially when Se levels were greater than the median (89.18 µg/L). Our findings support that Se can attenuate the negative associations of exposure to single Pb or the As, Cd, and Pb mixtures with cognitive function. Future prospective studies are needed to replicate our findings.

12.
J Int Med Res ; 50(12): 3000605221139668, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36482666

RESUMO

Brucellosis is a common zoonotic infectious disease worldwide that can affect almost all organs in the human body. Osteonecrosis of the femoral head has numerous possible causes. Patients usually experience movement disorders, severe pain, and an extremely high disability rate. We herein describe a 32-year-old male farmer living in Shanxi, China who accidentally injured his right hip while working. After 1 month of rest, the pain in the right hip was relieved, and the patient began to walk again. However, after 2 months of walking, he developed aggravated pain and claudication in the right lower limb. Examination revealed shortening of the right lower limb. We carried out a comprehensive and systematic examination and performed total hip arthroplasty. Hip trauma with Brucella infection may progress to osteonecrosis of the femoral head in a short period of time without typical symptoms of Brucella infection. Systematic examination and treatment should be performed at an early stage to prevent the eventual deterioration of the disease and serious complications that are difficult to treat.


Assuntos
Brucella , Brucelose , Necrose da Cabeça do Fêmur , Humanos , Adulto , Cabeça do Fêmur , Necrose da Cabeça do Fêmur/diagnóstico por imagem , Necrose da Cabeça do Fêmur/etiologia , Necrose da Cabeça do Fêmur/cirurgia , Caminhada , Brucelose/complicações
13.
Brain Behav ; : e2817, 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36409568

RESUMO

INTRODUCTION: Previous studies have shown that gestational inflammation can accelerate age-associated cognitive decline (AACD) in maternal mice; enriched environments (EEs) have been reported to protect normally aging mice from AACD and improve mitochondrial function. However, it is unclear whether the nitrosative stress-related proteins tet methylcytosine dioxygenase 1 (TET1) and S-nitrosoglutathione reductase (GSNOR) are involved in the accelerated aging process of gestational inflammation and whether EEs can slow this process. METHODS: In this study, CD-1 female mice on the 15th day of pregnancy were injected with bacterial lipopolysaccharide (50 µg/kg; LPS group) or an equivalent amount of normal saline (CON group) from the abdominal cavity for 4 consecutive days. Twenty-one days after delivery, half of the LPS-treated mice were randomly selected for EE until the end of the behavioral experiment (LPS-E group). When the female rats were raised to 6 months and 18 months of age, the Morris water maze (MWM) was used to detect spatial learning and memory ability; RT-PCR and Western blots were used to measure the mRNA and protein levels of hippocampal TET1 and GSNOR. RESULTS: As for the control group, compared with 6-month-old mice, the spatial learning and memory ability of 18-month-old mice decreased, and the hippocampal TET1 and GSNOR mRNA and protein levels were decreased. Gestational inflammation exacerbated these age-related changes, but an EE alleviated the effects. Pearson's correlation analysis indicated that performance during the learning and memory periods in the MWM correlated with the levels of hippocampal TET1 and GSNOR. CONCLUSIONS: Our findings suggest that gestational inflammation accelerates age-related learning and memory impairments and that postpartum EE exposure could alleviate these changes. These effects may be related to hippocampal TET1 and GSNOR expression.

14.
J Agric Food Chem ; 70(47): 14831-14840, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36383360

RESUMO

Hesperetin-7-O-glucoside (Hes-7-G) is a typical flavonoid monoglucoside, which can be generated from hesperidin with the removal of rhamnose by hydrolysis. Untargeted and targeted metabolomics together with 16S rRNA gene sequencing were employed to explore the exact absorption site of Hes-7-G and its beneficial effect in mice. Intestinal 1H nuclear magnetic resonance (NMR)-based metabolomics screening showed that Hes-7-G is mainly metabolized in the small intestine of mice, especially the ileum segment. Quantification analysis of bile acids (BAs) in the liver, intestinal tract, feces, and serum of mice suggests that Hes-7-G intake accelerates the processes of biosynthesis and excretion of BAs, thus promoting digestion and lowing hepatic cholesterol and triglyceride. 16S rRNA gene sequencing reveals that Hes-7-G significantly elevates the diversity of the gut microbiota in mice, especially those bacteria associated with BA secondary metabolism. These results demonstrated that long-term dietary Hes-7-G plays beneficial roles in health by modulating the gut bacteria and BA metabolism in mice.


Assuntos
Microbioma Gastrointestinal , Hesperidina , Camundongos , Animais , Microbioma Gastrointestinal/genética , Hesperidina/metabolismo , RNA Ribossômico 16S/genética , Ácidos e Sais Biliares/metabolismo , Fígado/metabolismo , Bactérias/genética , Bactérias/metabolismo , Glucosídeos/metabolismo , Camundongos Endogâmicos C57BL
15.
Int J Biol Macromol ; 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36395935

RESUMO

In this study, the immunity-enhancing effect of ginger polysaccharides UGP1 and UGP2 on CTX-induced immunosuppressed mice was evaluated. The results showed that ginger polysaccharide could effectively alleviate the symptoms of weight loss and dietary intake reduction induced by CTX, increase fecal water content, reduce fecal pH, and protect immune organs of immunosuppressed mice. In addition, ginger polysaccharides also stimulated the secretion of cytokines IL-2, IL-4, TNF-α and immunoglobulin Ig-G in the serum of mice, increased the expression of Occludin and Claudin-1, and restored the level of short-chain fatty acids in the intestine to improve immune deficiency. Furthermore, ginger polysaccharides significantly reduced the relative abundance ratio of the Firmicutes and Bacteroidetes in mice and increased the relative abundance of Verrucomicrobia and Bacteroidetes at the phylum level. At the family level, ginger polysaccharides increased the relative abundance of beneficial bacteria such as Muribaculaceae, Bacteroidaceae and Lactobacillaceae, and decreased the relative abundance of harmful bacteria such as Rikenellaceae and Lachnospiraceae. Spearman correlation analysis indicated that ginger polysaccharides could enhance intestinal immunity by modulating gut microbiota associated with immune function. These results indicated that ginger polysaccharides have the potential to be a functional food ingredients or a natural medicine for the treatment of intestinal barrier injury.

16.
J Agric Food Chem ; 70(46): 14732-14743, 2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36351282

RESUMO

The sugar moieties of natural flavonoids determine their absorption, bioavailability, and bioactivity in humans. To explore structure-dependent bioactivities of quercetin, isoquercetin, and rutin, which have the same basic skeleton linking different sugar moieties, we systemically investigated the ameliorative effects of dietary these flavonoids on high-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) of mice. Our results revealed that isoquercetin exhibits the strongest capability in improving NAFLD phenotypes of mice, including body and liver weight gain, glucose intolerance, and systemic inflammation in comparison with quercetin and rutin. At the molecular level, dietary isoquercetin markedly ameliorated liver dysfunction and host metabolic disorders in mice with NAFLD. At the microbial level, the three flavonoids compounds, especially isoquercetin, can effectively regulate the gut microbiota composition, such as genera Akkermansia, Bifidobacterium, and Lactobacillus, which were significantly disrupted in NAFLD mice. These comparative findings offer new insights into the structure-dependent activities of natural flavonoids for NAFLD treatment.


Assuntos
Microbioma Gastrointestinal , Hepatopatia Gordurosa não Alcoólica , Humanos , Camundongos , Animais , Hepatopatia Gordurosa não Alcoólica/metabolismo , Quercetina/farmacologia , Glicosídeos/farmacologia , Camundongos Endogâmicos C57BL , Rutina , Flavonoides/farmacologia , Açúcares
17.
Food Funct ; 13(22): 11518-11531, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36318047

RESUMO

The design of hypoallergenic derivatives is a new strategy for allergen-specific immunotherapy. Although hypoallergenic derivatives of Scylla paramamosain (mud crab) heat-stable tropomyosin (TM) and myosin light chain (MLC) have been preliminarily explored, their allergenicity in vivo needs to be further studied. In this work, recombinant allergens (wtTM, wtMLC) and hypoallergenic derivatives (mtTM, mtMLC) were purified. IgE-binding frequencies of wtTM and wtMLC in 177 crab-sensitised patients were 32.8% and 11.9%, respectively. In the Balb/c mouse model, mtTM and mtMLC caused mild intestinal inflammation, did not activate T-helper (Th) 2 immune response (interleukin-4, anaphylactic mediator, IgE, and IgG1 antibodies were not significantly increased) but could significantly promote the production of interleukin-10, which equilibrated Th1/Th2 cells, thus alleviating allergic symptoms. Moreover, mtTM and mtMLC-induced rabbit/mice anti-IgG antibodies could effectively block wtTM and wtMLC binding to patients' sera IgE in vitro. These results indicate that hypoallergenic derivatives offer the promise for an immunotherapeutic regimen for crab allergy.


Assuntos
Braquiúros , Hipersensibilidade Alimentar , Coelhos , Camundongos , Animais , Alérgenos , Camundongos Endogâmicos BALB C , Imunoglobulina E , Temperatura Alta , Imunoglobulina G , Hipersensibilidade Alimentar/terapia
18.
Huan Jing Ke Xue ; 43(11): 5274-5285, 2022 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-36437099

RESUMO

Soil microbial communities play an important role in driving a variety of ecosystem functions and ecological processes and are the primary driving force in maintaining the biogeochemical cycle. It has been observed that soil microbial diversity decreases with land use intensification and climate change in the global background. It is essential to investigate whether the reduction in soil microbial diversity can affect soil multifunctionality. Thus, in this study, the dilution-to-extinction method was used to construct the gradient of soil microbial diversity, combined with high-throughput sequencing to explore the impact of the reduction in bacterial, fungal, and protist diversity on soil multifunctionality. The results showed that the soil microbial alpha diversity (richness and Shannon index) was significantly lower than that of the original soil. Principal coordinate analysis (PCoA) showed that the microbial community structure of original soil was significantly different from that of diluted soil, and the response of bacterial and fungal communities to diluted soil was higher than that of protists. The regression model showed that there was a significant negative linear relationship between the average response value of soil multi-function and the index of microbial diversity, indicating that the change in soil microbial community was the key factor in regulating soil multifunctionality. The regression model showed that there was a significant negative linear relationship between soil multifunctionality and microbial diversity, indicating that the change in soil microbial community was the key factor to regulate soil multi-kinetic energy. Through the aggregated boosted tree analysis (ABT) and regression model, we found that some specific microbial groups, such as the Solacocozyma and Holtermaniella of fungi and Rudaea of bacteria, could significantly promote the change in soil multifunctionality, which showed that key microbial taxa play an indicative role in biological processes. Furthermore, the structural equation model revealed that bacteria could affect soil multifunctionality through the interaction between microbiomes, which was the key biological factor driving the change in soil multifunctionality. This study provided experimental evidence for the impact of soil microbial diversity on soil multifunctionality, and promoted the notion that maintaining a certain diversity of soil microbial community in a single agricultural ecosystem, especially the diversity of key microbial taxa, is of great significance to the sustainable development of ecosystem function in the future.


Assuntos
Microbiota , Solo , Solo/química , Microbiologia do Solo , Biodiversidade , Mudança Climática , Bactérias/genética
19.
Medicine (Baltimore) ; 101(41): e31002, 2022 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-36253999

RESUMO

BACKGROUND: The beneficial effects of dietary ß-carotene and vitamin A on Parkinson disease (PD) have been confirmed, but some studies have yielded questionable results. Therefore, this meta-analysis investigated the effect of dietary ß-carotene and vitamin A on the risk of PD. METHODS: The following databases were searched for relevant paper: PubMed, Embase, Medline, Scopus, Cochrane Library, CNKI, Wanfang Med online, and Weipu databases for the relevant paper from 1990 to March 28, 2022. The studies included were as follows: ß-carotene and vitamin A intake was measured using scientifically recognized approaches, such as food frequency questionnaire (FFQ); evaluation of odds ratios using OR, RR, or HR; ß-carotene and vitamin A intake for three or more quantitative categories; and PD diagnosed by a neurologist or hospital records. RESULTS: This study included 11 studies (four cohort studies, six case-control studies, and one cross-sectional study). The high ß-carotene intake was associated with a significantly lower chance of developing PD than low ß-carotene intake (pooled OR = 0.83, 95%CI = 0.74-0.94). Whereas the risk of advancement of PD was not significantly distinctive among the highest and lowest vitamin A intake (pooled OR = 1.08, 95%CI = 0.91-1.29). CONCLUSIONS: Dietary ß-carotene intake may have a protective effect against PD, whereas dietary vitamin A does not appear to have the same effect. More relevant studies are needed to include into meta-analysis in the further, as the recall bias and selection bias in retrospective and cross-sectional studies cause misclassifications in the assessment of nutrient intake.


Assuntos
Doença de Parkinson , beta Caroteno , Ácido Ascórbico , Estudos Transversais , Humanos , Metanálise como Assunto , Doença de Parkinson/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Revisões Sistemáticas como Assunto , Vitamina A , Vitamina E
20.
Int J Eat Disord ; 55(10): 1374-1383, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36184903

RESUMO

OBJECTIVE: This study examined the bidirectional relationships between sleep quality, loss of control (LOC) eating, and night eating in Chinese adolescents using longitudinal data over an 18-month study period. METHOD: Four-waves of data measurement (Waves 1-4), at 6-month intervals, were conducted with 2566 adolescents aged 11-17 years at baseline. A set of questionnaires were used to assess night eating, LOC eating, and sleep quality at each wave of data collection. Cross-lagged models were applied to analyze the bidirectional relationships between night eating, LOC eating, and sleep quality. RESULTS: Results indicated that higher night eating scores consistently predicted poorer sleep quality and higher LOC eating scores at Waves 1, 2, and 3. Furthermore, poorer sleep quality predicted higher night eating scores at Wave 1 and Wave 3, and higher LOC eating scores predicted higher night eating scores at Wave 1 and Wave 2. DISCUSSION: These findings highlight that night eating, LOC eating, and sleep quality were interrelated across time in Chinese adolescents. Improving sleep quality and reducing LOC eating might be promising in the prevention of night eating in adolescents. Similarly, reducing night eating might be promising in improving sleep quality and reducing LOC eating in adolescents. PUBLIC SIGNIFICANCE: This study explored the bidirectional relationship between night eating, LOC eating, and sleep quality in Chinese adolescents using cross-lagged models. Findings indicate bidirectional relationships between these variables and highlight the potential utility in incorporating sleep, LOC eating, and night eating interventions in eating pathology prevention designs for adolescents.


Assuntos
Transtornos da Alimentação e da Ingestão de Alimentos , Adolescente , China , Humanos , Qualidade do Sono , Inquéritos e Questionários
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