Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 218
Filtrar
1.
Polymers (Basel) ; 13(21)2021 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-34771191

RESUMO

Recently, lots of effort has been placed into stabilizing black phosphorus (BP) in the air to improve its compatibility with polymers. Herein, BP was chemically functionalized by aliphatic amine (DETA), aromatic amine (PPDA) and cyclamine (Pid) via a nucleophilic substitution reaction, aiming to develop an intensively reactive BP flame retardant for epoxy resin (EP). The -NH2 group on BP-DETA, BP-PPDA and BP-Pid reacted with the epoxide group at different temperatures. The lowest temperature was about 150 °C for BP-DETA. The impacts of three BP-NH2 were compared on the flame retardancy and thermal decomposition of EP. At 5 wt% loading, EP/BP-NH2 all passed UL 94 V 0 rating. The limiting oxygen index (LOI) of EP/BP-PPDA was as high as 32.3%. The heat release rate (HRR) of EP/BP-DETA greatly decreased by 46% and char residue increased by 73.8%, whereas HRR of EP/BP-Pid decreased by 11.5% and char residue increased by 50.8%, compared with EP. Average effective heat of combustion (av-EHC) of EP/BP-Pid was lower than that of EP/BP-DETA and EP/BP-PPDA. In view of the flame-retardant mechanism, BP nanosheets functionalized with aliphatic amine and aromatic amine played a dominant role in the condensed phase, while BP functionalized with cyclamine was more effective in the gas phase.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34839662

RESUMO

Poly(ethylene oxide) (PEO)-based composite solid electrolytes (CSEs) are considered as one of the most promising candidates for all-solid-state lithium batteries (ASSLBs). However, a key challenge for their further development is to solve the main issues of low ionic conductivity and poor mechanical strength, which can lead to insufficient capacity and stability. Herein, ß-cyclodextrin (ß-CD) is first demonstrated as a multifunctional filler that can form a continuous hydrogen bond network with the ether oxygen unit from the PEO matrix, thus improving the comprehensive performances of the PEO-based CSE. By relevant characterizations, it is demonstrated that ß-CD is uniformly dispersed into the PEO substrate, inducing adequate dissociation of lithium salt and enhancing mechanical strength through hydrogen bond interactions. In a Li/Li symmetric battery, the ß-CD-integrated PEO-based (PEO-LiTFSI-15% ß-CD) CSE works well at a critical current density up to 1.0 mA cm-2 and retains stable lithium plating/stripping for more than 1000 h. Such reliable properties also enable its superior performance in LiFePO4-based ASSLBs, with specific capacities of 123.6 and 114.0 mA h g-1 as well as about 100 and 81.8% capacity retention over 300 and 700 cycles at 1 and 2 C (1 C = 170 mA g-1), respectively.

3.
J Colloid Interface Sci ; 608(Pt 1): 470-481, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34628315

RESUMO

The sluggish redox kinetic and shuttle effect of polysulfides still obstruct the commercial application of lithium-sulfur (Li-S) batteries. Herein, a nanocomposite consisting of well-dispersed and lamellar-like shape CoS anchored on g-C3N4 nanosheets (CoS@g-C3N4) is prepared firstly, and then it is integrated on a polypropylene membrane combined with little conductive Ketjen black (KB) to fabricate a multifunctional and quite thin interlayer, with a thickness of only âˆ¼ 2.1 um and areal mass loading of âˆ¼ 0.07 mg·cm-2. The as-prepared interlayer firstly can capture polysulfides by Li-N bond as well as Lewis acid-base interaction between CoS and polysulfide anions (Sn2-), and more importantly, it also displays a positive effect on catalyzing the redox conversion of intermediate polysulfides. As expected, a Li-S cell assembled with this modified separator and high sulfur content cathode displays an excellent electrochemical performance, with specific capacity of âˆ¼ 1290 mAh g-1 at 0.2C and a low fading rate of 0.03% per cycle after 500 cycles at 1.0C. Furthermore, a high sulfur mass loading of âˆ¼ 4.0 mg·cm-2 electrode paired with this multifunctional separator exhibits a stable specific capacity of âˆ¼ 600 mAh g-1 after 250 cycles under 0.1C. This work can give some guides to rational design a quite thin and light interlayer for improving the utilization of sulfur species, with little damage to the energy density and Li ion transportation in Li-S batteries.

4.
Adv Mater ; : e2107326, 2021 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-34699633

RESUMO

The worldwide energy demand in electric vehicles and the increasing global temperature have called for development of high-energy and long-life lithium-ion batteries (LIBs) with improved high-temperature operational resiliency. However, current attention has been mostly focused on cycling aging at elevated temperature, leaving considerable gaps of knowledge in the failure mechanism, and practical control of abusive calendar aging and thermal runaway that are highly related to the eventual operational lifetime and safety performance of LIBs. Herein, using a combination of various in situ synchrotron X-ray and electron microscopy techniques, a multiscale understanding of surface structure effects involved in regulating the high-temperature operational tolerance of polycrystalline Ni-rich layered cathodes is reported. The results collectively show that an ultraconformal poly(3,4-ethylenedioxythiophene) coating can effectively prevent a LiNi0.8 Co0.1 Mn0.1 O2 cathode from undergoing undesired phase transformation and transition metal dissolution on the surface, atomic displacement, and dislocations within primary particles, intergranular cracking along the grain boundaries within secondary particles, and intensive bulk oxygen release during high state-of-charge and high-temperature aging. The present work highlights the essential role of surface structure controls in overcoming the multiscale degradation pathways of high-energy battery materials at extreme temperature.

5.
ACS Appl Mater Interfaces ; 13(43): 51218-51229, 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34672188

RESUMO

Environmentally friendly lead-free dielectric ceramics have attracted wide attention because of their outstanding power density, rapid charge/dischargerate, and superior stability. Nevertheless, as a hot material in dielectric ceramic capacitors, the energy storage performance of Na0.5Bi0.5TiO3-based ceramics has been not satisfactory because of their higher remnant polarization value and low dielectric breakdown strength, which is a problem that must be urgently overcome. In this work, the (1 - x) (0.6Na0.5Bi0.5TiO3 - 0.4Sr0.7Bi0.2TiO3) - xBa(Mg1/3Ta2/3)O3 (BNST-xBMT) systems were designed based on a dual optimization strategy of domain and bandgap to solve the above problems. As a result, a record-breaking ultrahigh energy density and excellent efficiency (Wrec = 8.58 J/cm3, η = 93.5%) were obtained simultaneously under 565 kV/cm for the BNST-0.08BMT ceramic. The introduction of Sr0.7Bi0.2TiO3 induces the formation of nanodomains in BNT-based ceramics, leading to slim P-E curves, and the further modification of Mg/Ta reduces the grain sizes and increases the bandgap width, resulting in significant enhancement of the dielectric breakdown strength. Moreover, excellent stability and superior discharge performance (Wd = 4.7 J/cm3, E = 320 kV/cm) in the BNST-0.08BMT ceramic were also achieved. The results suggest that the BNST-0.08BMT ceramic shows potential applicability for dielectric energy storage ceramics. Simultaneously, the composition-design concept in the system provides a good reference for the further development of ceramic dielectric capacitors.

6.
Nanomaterials (Basel) ; 11(10)2021 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-34685178

RESUMO

MXenes are a new class of two-dimensional (2D) materials with promising applications in many fields because of their layered structure and unique performance. In particular, the physical barrier properties of two-dimensional nanosheets make them suitable as barriers against hydrogen. Herein, MXene coatings were prepared on pipe steel by a simple spin-coating process with a colloidal suspension. The hydrogen resistance was evaluated by electrochemical hydrogen permeation tests and slow strain rate tests, and the corrosion resistance was assessed by potentiodynamic polarization. The results reveal that MXene coatings offer excellent hydrogen resistance and corrosion protection by forming a barrier against diffusion. Experimentally, the hydrogen permeability of the MXene coating is one third of the substrate, and the diffusion coefficient decreases as well. The mechanistic study indicates that the hydrogen resistance of the MXene coatings is affected by the number of spin-coated layers, while the concentration of the d-MXene colloidal suspension determines the thickness of a single coating. However, damage to the sample surface caused by the colloidal suspension that contains H+ and F- may limit the improvement of the hydrogen resistance. This paper reveals a new application of 2D MXene materials as a novel efficient barrier against hydrogen permeation and the subsequent alleviation of hydrogen embrittlement in the steel substrate.

7.
Pest Manag Sci ; 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34523221

RESUMO

BACKGROUND: The use of chemical insecticides to control Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) is widespread, although it might exert a sublethal effect on its dominant parasitoid, Encarsia formosa Gahan (Hymenoptera: Aphelinidae). To investigate the sublethal effect of spirotetramat on E. formosa, we observed the ability of E. formosa to locate and handle the host, oviposit and preen after exposure to sublethal concentrations of spirotetramat. RESULTS: After exposure to spirotetramat at LC50 , the response time of E. formosa to the volatile reached 223.40 s and was significantly prolonged. Only 56.44% of the wasps were attracted by the volatile and the insect crawled the slowest among all of the treatments. The averages of oviposition posture adopted and host handled by each E. formosa in 1 h decreased significantly to 1.79 and 1.27, respectively. At the sublethal concentration of LC10 , 94.59% of the wasps were attracted by the volatile and the insect crawled the fastest. The average of host handled by each E. formosa was 3.92, and the frequency of drumming while walking and drumming the host was 12.34 times per second and 12.30 times per second, respectively, demonstrating a significant acceleration in these abilities. CONCLUSION: These findings demonstrate that spirotetramat induced hormesis in E. formosa on exposure to its LC10 concentration and accelerated its host locating, host handling and frequency of antennae drumming. These findings could assist in balancing the chemical and biological control of B. tabaci and enhancing the efficacy of E. formosa as a biocontrol agent.

8.
Physiol Rep ; 9(15): e14991, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34342168

RESUMO

In both humans and rodent models, circulating glycine levels are significantly reduced in obesity, glucose intolerance, type II diabetes, and non-alcoholic fatty liver disease. The glycine cleavage system and its rate-limiting enzyme, glycine decarboxylase (GLDC), is a major determinant of plasma glycine levels. The goals of this study were to determine if the increased expression of GLDC contributes to the reduced plasma glycine levels seen in disease states, to characterize the hormonal regulation of GLDC gene expression, and to determine if altered GLDC expression has physiological effects that might affect the development of diabetes. The findings presented here show that hepatic GLDC gene expression is elevated in mouse models of obesity and diabetes, as well as by fasting. We demonstrated that GLDC gene expression is strongly regulated by the metabolic hormones glucagon and insulin, and we identified the signaling pathways involved in this regulation. Finally, we found that GLDC expression is linked to glutathione levels, with increased expression associated with elevated levels of glutathione and reduced expression associated with a suppression of glutathione and increased cellular ROS levels. These findings suggest that the hormonal regulation of GLDC contributes not only to the changes in circulating glycine levels seen in metabolic disease, but also affects glutathione production, possibly as a defense against metabolic disease-associated oxidative stress.

9.
Clin Interv Aging ; 16: 1439-1447, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34335022

RESUMO

Purpose: The early detection of major post-stroke depression (PSD) is essential to optimize patient care. A major PSD prediction tool needs to be developed and validated for early screening of major PSD patients. Patients and Methods: A total of 639 acute ischemic stroke (AIS) patients from three hospitals were consecutively recruited and completed a 3-month follow-up. Sociodemographic, clinical and laboratory test data were collected on admission. With major depression criteria being met in the DSM-V, 17-item Hamilton Rating Scale For Depression (HRSD) score ≥17 at 3 months after stroke onset was regarded as the primary endpoint. Multiple imputation was used to substitute the missing values and multivariable logistic regression model was fitted to determine associated factors with a bootstrap backward selection process. The nomogram was constructed based on the regression coefficients of the associated factors. Performance of the nomogram was assessed by discrimination (C-statistics) and calibration curve. Results: A total of 7.04% (45/639) of patients were diagnosed with major PSD at 3 months. The final logistic regression model included age, baseline NIHSS and mRS scores, educational level, calcium-phosphorus product, history of hypertension and atrial fibrillation. The model had acceptable discrimination, based on a C-statistic of 0.81 (95% CI, 0.791-0.829), with 71.1% sensitivity and 78.6% specificity. We also transformed the model to a nomogram, an easy-to-use clinical tool which could be used to facilitate the early screening of major PSD patients at 3 months. Conclusion: We identified several associated factors of major PSD at 3 months and constructed a convenient nomogram to guide follow-up and aid accurate prognostic assessment.


Assuntos
Isquemia Encefálica , Acidente Vascular Cerebral , Idoso , Isquemia Encefálica/complicações , Depressão/diagnóstico , Depressão/epidemiologia , Depressão/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nomogramas , Acidente Vascular Cerebral/complicações
10.
J BUON ; 26(3): 1181, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34268998

RESUMO

The Editors of JBUON issue an Expression of Concern to 'Antiproliferative potential of piperine and curcumin in drug-resistant human leukemia cancer cells are mediated via autophagy and apoptosis induction, S-phase cell cycle arrest and inhibition of cell invasion and migration', by Ning Li, Shuyun Wen, Guohua Chen, Shijun Wang; JBUON 2020;25(1):401-406; PMID: 32277661. Following the publication of the above article, readers drew to our attention that part of the data was possibly unreliable. We sent emails to the authors with a request to provide the raw data to prove the originality, but received no reply. Therefore, as we continue to work through the issues raised, we advise readers to interpret the information presented in the article with due caution. We thank the readers for bringing this matter to our attention. We apologize for any inconvenience it may cause.

11.
Pediatr Blood Cancer ; 68(8): e29097, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34031980

RESUMO

OBJECTIVE: Cytokine storms are central to the development of Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis (EBV-HLH). Previous studies have shown that single-nucleotide polymorphisms (SNPs) of cytokine genes may be associated with the development of EBV-HLH in children. As such, we investigated the association between susceptibility to EBV-HLH in children and SNPs and haplotypes of genes encoding interleukin-2 receptor subunit alpha (IL2RA), interleukin-10 (IL10), interferon gamma (IFNG), interferon regulatory factor 5 (IRF5), and C-C chemokine receptor 2 (CCR2). METHODS: Sixty-six children with EBV-HLH and 58 healthy EBV-seropositive controls were enrolled in this study. SNPs of IL2RA rs2104286, rs12722489, and rs11594656; IL10 rs1800896, rs1800871, and rs1800872; IFNG rs2430561, IRF5 rs2004640, and CCR2 rs1799864 were assayed and genotyped using the SNaPshot technique. RESULTS: Frequencies of the A allele of IL2RA rs2104286 and IL10 rs1800896, and C allele of IL-10 rs1800872 were significantly higher in the EBV-HLH group than in the control group. The AA genotype of IL2RA rs2104286 and IL10 rs1800896, and the CC genotype of IL10 rs1800872 might be associated with a significantly high risk of EBV-HLH. However, the frequencies of genotypes and alleles of IL2RA rs2104286, IL10 rs1800871, IFNG rs2430561, IRF5 rs2004640, and CCR2 rs1799864 were similar in both groups. Additionally, IL2RA AGT (rs2104286-rs12722489-rs11594656) and IL10 ACC (rs1800896-rs1800871-rs1800872) haplotypes were also associated with an increased risk of EBV-HLH. CONCLUSIONS: SNPs of IL2RA rs2104286, IL10 rs1800896 and rs1800872 and the haplotypes of IL2RA AGT and IL10 ACC were highly associated with susceptibility to EBV-HLH in children.

12.
Cell Death Dis ; 12(6): 549, 2021 05 26.
Artigo em Inglês | MEDLINE | ID: mdl-34039960

RESUMO

Hepatocellular carcinoma (HCC) is a common primary liver malignancy lacking effective molecularly-targeted therapies. HBO1 (lysine acetyltransferase 7/KAT7) is a member of MYST histone acetyltransferase family. Its expression and potential function in HCC are studied. We show that HBO1 mRNA and protein expression is elevated in human HCC tissues and HCC cells. HBO1 expression is however low in cancer-surrounding normal liver tissues and hepatocytes. In HepG2 and primary human HCC cells, shRNA-induced HBO1 silencing or CRISPR/Cas9-induced HBO1 knockout potently inhibited cell viability, proliferation, migration, and invasion, while provoking mitochondrial depolarization and apoptosis induction. Conversely, ectopic overexpression of HBO1 by a lentiviral construct augmented HCC cell proliferation, migration and invasion. In vivo, xenografts-bearing HBO1-KO HCC cells grew significantly slower than xenografts with control HCC cells in severe combined immunodeficient mice. These results suggest HBO1 overexpression is important for HCC cell progression.


Assuntos
Carcinoma Hepatocelular/genética , Histona Acetiltransferases/metabolismo , Neoplasias Hepáticas/genética , Animais , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Feminino , Humanos , Neoplasias Hepáticas/patologia , Camundongos , Camundongos SCID
13.
Tumor Microenviron ; 1(2): 55-62, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33869785

RESUMO

Context/Aims: Metabolic reprogramming and cellular plasticity drive tumorigenesis. However, how these cellular events collectively contribute to the oncogenic process is poorly understood. Epithelial-mesenchymal transition (EMT), a fundamental mechanism of cellular plasticity, is governed by the EMT transcription repressors such as Snail. In the present study, through establishment and characterization of inducible overexpression of Snail in A549 lung cancer cells, we aim to define the metabolic reprogramming in response to Snail in the EMT of lung cancer cells. Methods/Results: Our metabolomic analysis suggests that forced expression of Snail accompanied reduced diversion of glycolytic metabolites to the serine/glycine metabolic shunt, a critical metabolic branch that distributes glucose catabolic intermediates to the major anabolic pathways. Our gene expression profiling and molecular characterization revealed that Snail actively suppressed the expression of glycine decarboxylase (GLDC), a key enzyme on the serine/glycine metabolic shunt, through binding to an evolutionarily conserved E-box motif and thereby inhibiting the promoter of the GLDC gene. Besides, knockdown of GLDC led to a cellular function shift from proliferation to migration. Conclusion: This study has revealed a novel molecular link that integrates the serine/glycine metabolism with the Snail-mediated EMT program in cancer cells.

14.
Front Neurorobot ; 15: 621196, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33776677

RESUMO

This study was developed to explore the role of the intelligent badminton training robot (IBTR) to prevent badminton player injuries based on the machine learning algorithm. An IBTR is designed from the perspectives of hardware and software systems, and the movements of the athletes are recognized and analyzed with the hidden Markov model (HMM) under the machine learning. After the design was completed, it was simulated with the computer to analyze its performance. The results show that after the HMM is optimized, the recognition accuracy or data pre-processing algorithm, based on the sliding window segmentation at the moment of hitting reaches 96.03%, and the recognition rate of the improved HMM to the robot can be 94.5%, showing a good recognition effect on the training set samples. In addition, the accuracy rate is basically stable when the total size of the training data is 120 sets, after the accuracy of the robot is analyzed through different data set sizes. Therefore, it was found that the designed IBTR has a high recognition rate and stable accuracy, which can provide experimental references for injury prevention in athlete training.

15.
Curr Med Res Opin ; 37(6): 917-927, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33729889

RESUMO

BACKGROUND: To develop a sensitive and clinically applicable risk assessment tool identifying coronavirus disease 2019 (COVID-19) patients with a high risk of mortality at hospital admission. This model would assist frontline clinicians in optimizing medical treatment with limited resources. METHODS: 6415 patients from seven hospitals in Wuhan city were assigned to the training and testing cohorts. A total of 6351 patients from another three hospitals in Wuhan, 2169 patients from outside of Wuhan, and 553 patients from Milan, Italy were assigned to three independent validation cohorts. A total of 64 candidate clinical variables at hospital admission were analyzed by random forest and least absolute shrinkage and selection operator (LASSO) analyses. RESULTS: Eight factors, namely, Oxygen saturation, blood Urea nitrogen, Respiratory rate, admission before the date the national Maximum number of daily new cases was reached, Age, Procalcitonin, C-reactive protein (CRP), and absolute Neutrophil counts, were identified as having significant associations with mortality in COVID-19 patients. A composite score based on these eight risk factors, termed the OURMAPCN-score, predicted the risk of mortality among the COVID-19 patients, with a C-statistic of 0.92 (95% confidence interval [CI] 0.90-0.93). The hazard ratio for all-cause mortality between patients with OURMAPCN-score >11 compared with those with scores ≤ 11 was 18.18 (95% CI 13.93-23.71; p < .0001). The predictive performance, specificity, and sensitivity of the score were validated in three independent cohorts. CONCLUSIONS: The OURMAPCN score is a risk assessment tool to determine the mortality rate in COVID-19 patients based on a limited number of baseline parameters. This tool can assist physicians in optimizing the clinical management of COVID-19 patients with limited hospital resources.


Assuntos
COVID-19 , Medição de Risco/métodos , COVID-19/epidemiologia , COVID-19/mortalidade , China , Hospitalização/estatística & dados numéricos , Humanos , Itália , Fatores de Risco
16.
Stroke Vasc Neurol ; 6(2): 291-297, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33443231

RESUMO

BACKGROUND AND PURPOSE: Stroke is the second leading cause of death worldwide and the leading cause of mortality and long-term disability in China, but its underlying risk genes and pathways are far from being comprehensively understood. We here describe the design and methods of whole genome sequencing (WGS) for 10 914 patients with acute ischaemic stroke or transient ischaemic attack from the Third China National Stroke Registry (CNSR-III). METHODS: Baseline clinical characteristics of the included patients in this study were reported. DNA was extracted from white blood cells of participants. Libraries are constructed using qualified DNA, and WGS is conducted on BGISEQ-500 platform. The average depth is intended to be greater than 30× for each subject. Afterwards, Sentieon software is applied to process the sequencing data under the Genome Analysis Toolkit best practice guidance to call genotypes of single nucleotide variants (SNVs) and insertion-deletions. For each included subject, 21 fingerprint SNVs are genotyped by MassARRAY assays to verify that DNA sample and sequencing data originate from the same individual. The copy number variations and structural variations are also called for each patient. All of the genetic variants are annotated and predicted by bioinformatics software or by reviewing public databases. RESULTS: The average age of the included 10 914 patients was 62.2±11.3 years, and 31.4% patients were women. Most of the baseline clinical characteristics of the 10 914 and the excluded patients were balanced. CONCLUSIONS: The WGS data together with abundant clinical and imaging data of CNSR-III could provide opportunity to elucidate the molecular mechanisms and discover novel therapeutic targets for stroke.

17.
Virol J ; 18(1): 27, 2021 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-33499896

RESUMO

BACKGROUND: Orf virus (ORFV) is a member of the genus Parapoxvirus and family Poxviridae. The virus has a worldwide distribution and infects sheep, goats, humans, and wild animals. However, due to the complex structure of the poxvirus, the underlying mechanism of the entry and infection by ORFV remains largely unknown. ORFV ORF047 encodes a protein named L1R. Poxviral L1R serves as the receptor-binding protein and blocks virus binding and entry independently of glycosaminoglycans (GAGs). The study aimed to identify the host interaction partners of ORFV ORF047. METHODS: Yeast two-hybrid cDNA library of sheep testicular cells was applied to screen the host targets with ORF047 as the bait. ORF047 was cloned into a pBT3-N vector and expressed in the NMY51 yeast strain. Then, the expression of bait proteins was validated by Western blot analysis. RESULTS: Sheep SERP1and PABPC4 were identified as host target proteins of ORFV ORF047, and a Co-IP assay further verified their interaction. CONCLUSIONS: New host cell proteins SERP1and PABPC4 were found to interact with ORFV ORF047 and might involve viral mRNA translation and replication.


Assuntos
Interações entre Hospedeiro e Microrganismos , Vírus do Orf/metabolismo , Proteínas do Envelope Viral/metabolismo , Animais , Células Cultivadas , Masculino , Proteínas de Membrana/metabolismo , Vírus do Orf/química , Vírus do Orf/genética , Ligação Proteica , Ovinos/virologia , Testículo/citologia , Proteínas do Envelope Viral/análise , Proteínas do Envelope Viral/genética
18.
J Am Chem Soc ; 143(5): 2433-2440, 2021 02 10.
Artigo em Inglês | MEDLINE | ID: mdl-33507070

RESUMO

Ammonia electro-oxidation is an extremely significant reaction with regards to the nitrogen cycle, hydrogen economy, and wastewater remediation. The design of efficient electrocatalysts for use in the ammonia electro-oxidation reaction (AOR) requires comprehensive understanding of the mechanism and intermediates involved. In this study, aggregation-induced emission (AIE), a robust fluorescence sensing platform, is employed for the sensitive and qualitative detection of hydrazine (N2H4), one of the important intermediates during the AOR. Here, we successfully identified N2H4 as a main intermediate during the AOR on the model Pt/C electrocatalyst using 4-(1,2,2-triphenylvinyl)benzaldehyde (TPE-CHO), an aggregation-induced emission luminogen (AIEgen). We propose the AOR mechanism for Pt with N2H4 being formed during the dimerization process (NH2 coupling) within the framework of the Gerischer and Mauerer mechanism. The unique chemodosimeter approach demonstrated in this study opens a novel pathway for understanding electrochemical reactions in depth.

19.
Med (N Y) ; 2(1): 38-48.e2, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33043313

RESUMO

Background: The coronavirus disease 2019 (COVID-19) is a recently emerged respiratory infectious disease with kidney injury as a part of the clinical complications. However, the dynamic change of kidney function and its association with COVID-19 prognosis are largely unknown. Methods: In this multicenter retrospective cohort study, we analyzed clinical characteristics, medical history, laboratory tests, and treatment data of 12,413 COVID-19 patients. The patient cohort was stratified according to the severity of the outcome into three groups: non-severe, severe, and death. Findings: The prevalence of elevated blood urea nitrogen (BUN), elevated serum creatinine (Scr), and decreased blood uric acid (BUA) at admission was 6.29%, 5.22%, and 11.66%, respectively. The trajectories showed the elevation in BUN and Scr levels, as well as a reduction in BUA level for 28 days after admission in death cases. Increased all-cause mortality risk was associated with elevated baseline levels of BUN and Scr and decreased levels of BUA. Conclusions: The dynamic changes of the three kidney function markers were associated with different severity and poor prognosis of COVID-19 patients. BUN showed a close association with and high potential for predicting adverse outcomes in COVID-19 patients for severity stratification and triage. Funding: This study was supported by grants from the National Key R&D Program of China (2016YFF0101504), the National Science Foundation of China (81630011, 81970364, 81970070, 81970011, 81870171, and 81700356), the Major Research Plan of the National Natural Science Foundation of China (91639304), the Hubei Science and Technology Support Project (2019BFC582, 2018BEC473, and 2017BEC001), and the Medical Flight Plan of Wuhan University.

20.
J Biol Chem ; 296: 100104, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33219129

RESUMO

ABHD5 is an essential coactivator of ATGL, the rate-limiting triglyceride (TG) lipase in many cell types. Importantly, ABHD5 also functions as a tumor suppressor, and ABHD5 mRNA expression levels correlate with patient survival for several cancers. Nevertheless, the mechanisms involved in ABHD5-dependent tumor suppression are not known. We found that overexpression of ABHD5 induces cell cycle arrest at the G1 phase and causes growth retardation in a panel of prostate cancer cells. Transcriptomic profiling and biochemical analysis revealed that genetic or pharmacological activation of lipolysis by ABHD5 potently inhibits mTORC1 signaling, leading to a significant downregulation of protein synthesis. Mechanistically, we found that ABHD5 elevates intracellular AMP content, which activates AMPK, leading to inhibition of mTORC1. Interestingly, ABHD5-dependent suppression of mTORC1 was abrogated by pharmacological inhibition of DGAT1 or DGAT2, isoenzymes that re-esterify fatty acids in a process that consumes ATP. Collectively, this study maps out a novel molecular pathway crucial for limiting cancer cell proliferation, in which ABHD5-mediated lipolysis creates an energy-consuming futile cycle between TG hydrolysis and resynthesis, leading to inhibition of mTORC1 and cancer cell growth arrest.


Assuntos
1-Acilglicerol-3-Fosfato O-Aciltransferase/metabolismo , Proteínas Quinases Ativadas por AMP/metabolismo , Alvo Mecanístico do Complexo 1 de Rapamicina/metabolismo , 1-Acilglicerol-3-Fosfato O-Aciltransferase/genética , Monofosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Western Blotting , Ciclo Celular/genética , Ciclo Celular/fisiologia , Proliferação de Células/genética , Proliferação de Células/fisiologia , Humanos , Lipólise/fisiologia , Metabolismo/fisiologia , RNA-Seq
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...