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1.
PLoS One ; 14(3): e0210254, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30865636

RESUMO

A new species, Manglietia pubipedunculata Q. W. Zeng & X. M. Hu (Magnoliaceae) is described and illustrated from Yunnan, China. In addition to macromorphological examination, we comparatively studied on micromorphology of leaf epidermis, leaf structure, and epidermal cell on the sclerotesta. This new species is similar to M. kwangtungensis in terms of having dense pubescence, however, their pubescence are quite different. Manglietia pubipedunculata has appressed, compressed, shorter and sparser pubescence consisting of single or two cells. Moreover, it differs from M. kwangtungensis by showing shorter and thicker peduncles, longer styles, basal carpels covered with sparsely brown appressed pubescence, and more ovules per carpel. Furthermore, the new species has thinner leaves, brown and rugged surfaces on sclerotesta, and the alveolate cell pattern consisting of pentagon or hexagon cells with papilla on secondary cell wall under the observation by SEM. The phylogenetic analysis from two nuclear PHYA and LEAFY and chloroplast trnH-psbA sequences of 11 taxa reveals that M. pubipedunculata is a distinct species.

2.
PLoS One ; 13(1): e0189034, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29329302

RESUMO

Bretschneidera sinensis, a class-I protected wild plant in China, is a relic of the ancient Tertiary tropical flora endemic to Asia. However, little is known about its genetics and phylogeography. To elucidate the current phylogeographic patterns and infer the historical population dynamics of B. sinensis, and to make recommendations for its conservation, three non-coding regions of chloroplast DNA (trnQ-rps16, rps8-rps11, and trnT-trnL) were amplified and sequenced across 256 individuals from 23 populations of B. sinensis, spanning 10 provinces of China. We recognized 13 haplotypes, demonstrating relatively high total haplotype diversity (hT = 0.739). Almost all of the variation existed among populations (98.09%, P < 0.001), but that within populations was low (1.91%, P < 0.001). Strong genetic differentiation was detected among populations (GST = 0.855, P < 0.001) with limited estimations of seed flow (Nm = 0.09), indicating that populations were strongly isolated from one another. According to SAMOVA analysis, populations of B. sinensis in China could be divided into five geographic groups: (1) eastern Yunnan to western Guangxi; (2) Guizhou-Hunan-Hubei; (3) central Guangdong; (4) northwestern Guangdong; and (5) the Luoxiao-Nanling-Wuyi -Yangming Mountain. Network analysis showed that the most ancestral haplotypes were located in the first group, i.e., the eastern Yungui Plateau and in eastern Yunnan, which is regarded as a putative glacial refugia for B. sinensis in China. B. sinensis may have expanded its range eastward from these refugia and experienced bottleneck or founder effects in southeastern China. Populations in Liping (Guizhou Province), Longsheng (Guangxi Province), Huizhou (Guangdong Province), Chongyi (Jiangxi Province), Dong-an (Hunan Province), Pingbian (Yunnan Province) and Xinning (Hunan Province) are proposed as the priority protection units.


Assuntos
Brassicaceae/classificação , Brassicaceae/genética , Conservação dos Recursos Naturais , Genes de Plantas , Filogeografia , China , DNA de Cloroplastos/genética , Variação Genética , Haplótipos , Reação em Cadeia da Polimerase
3.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 37(2): 174-178, 2017 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30650269

RESUMO

Objective To observe the correlation between syndrome types of Chinese medicine (CM) and molecular subtyping of breast cancer in consolidation period. Methods Multivariate statistical analysis was applied in this research. Totally 612 breast cancer patients in consolidation period were as- signed to 4 syndrome types of CM [qi deficiency syndrome (22. 22% , 136/612) , Gan-qi stagnation syn- drome (21.73%, 133/612), Gan-Shen yin deficiency syndrome (20.10%, 123/612), Shen deficiency blood stasis syndrome (35. 95%, 220/612) ]. The correlation between each syndrome type and each molecular subtyping was respectively analyzed using Χ² (R x C table) test. Results Through statistical a- nalysis and expert consultation, results showed that cluster four types in consolidate period of breast cancer were compatible. Each syndrome type was correlated with each molecular subtyping in 612 breast cancer patients in consolidation period. Luminal type A was correlated with Gan-qi stagnation syndrome (P <0. 05). Luminal type B and triple negative type were correlated with qi deficiency syndrome and Gan- Shen yin deficiency syndrome (P <0. 05). But each syndrome type was not obviously correlated with dis- ease course. There was no obvious correlation between molecular subtyping and age/disease course (P >0. 05). Conclusion Luminal type A occupied the highest ratio in Gan-qi stagnation syndrome, with relatively better prognosis.


Assuntos
Neoplasias da Mama , Correlação de Dados , Medicina Tradicional Chinesa , Neoplasias da Mama/classificação , Neoplasias da Mama/terapia , Humanos , Síndrome , Deficiência da Energia Yin
5.
BMC Plant Biol ; 15: 36, 2015 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-25652180

RESUMO

BACKGROUND: There are different opinions about the relationship of two disjunctively distributed varieties Adiantum reniforme L. var. sinense Y.X.Lin and Adiantum reniforme L. Adiantum reniforme var. sinense is an endangered fern only distributed in a narrowed region of Chongqing city in China, while Adiantum reniforme var. reniforme just distributed in Canary Islands and Madeira off the north-western African coast. To verify the relationship of these two taxa, relative phylogenetic analyses, karyotype analyses, microscopic spore observations and morphological studies were performed in this study. Besides, divergence time between A. reniforme var. sinense and A. reniforme var. reniforme was estimated using GTR model according to a phylogeny tree constructed with the three cpDNA markers atpA, atpB, and rbcL. RESULTS: Phylogenetic results and divergence time analyses--all individuals of A. reniforme var. sinense from 4 different populations (representing all biogeographic distributions) were clustered into one clade and all individuals of A. reniforme var. reniforme from 7 different populations (all biogeographic distributions are included) were clustered into another clade. The divergence between A. reniforme var. reniforme and A. reniforme var. sinense was estimated to be 4.94 (2.26-8.66) Myr. Based on karyotype analyses, A. reniforme var. reniforme was deduced to be hexaploidy with 2n = 180, X = 30, while A. reniforme var. sinense was known as tetraploidy. Microscopic spore observations suggested that surface ornamentation of A. reniforme var. reniforme is psilate, but that of A. reniforme var. sinense is rugate. Leaf blades of A. reniforme var. sinense are membranous and reniform and with several obvious concentric rings, and leaves of A. reniforme var. reniforme are pachyphyllous and coriaceous and are much rounder and similar to palm. CONCLUSION: Adiantum reniforme var. sinense is an independent species rather than the variety of Adiantum reniforme var. reniforme. As a result, we approve Adiantum nelumboides X. C. Zhang, nom. & stat. nov. as a legal name instead of the former Adiantum reniforme var. sinense. China was determined to be the most probable evolution centre based on the results of phylogenetic analyses, divergence estimation, relative palaeogeography and palaeoclimate materials.


Assuntos
Adiantum/classificação , Adiantum/genética , China , Cromossomos de Plantas , DNA de Plantas/genética , Marcadores Genéticos , Cariotipagem , Microscopia Eletrônica de Varredura , Dados de Sequência Molecular , Filogenia , Espanha
6.
Artigo em Inglês | MEDLINE | ID: mdl-25389442

RESUMO

Yiqi formula (YF), a traditional herbal prescription, has long been used to treat triple-negative breast cancer (TNBC) patients. The present study aims to investigate the effects and the related mechanism of YF for treatment of TNBC xenografts. MDA-MB-231 (human TNBC) cells were subcutaneously injected into the second mammary fat pad of 40 female nude mice, which were divided into four groups: control, erlotinib (an epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor), YF, and combination (YF plus erlotinib). All treatments were administered orally for 30 days. Inhibition rate of tumor weight by erlotinib, YF, and the combination was 26.47%, 17.24%, and 39.15%, respectively. Western blotting showed that YF, erlotinib, and the combination downregulated p-EGFR (P < 0.01) and p-Akt1 (pT308) (P < 0.05) and upregulated PTEN compared with control, and the combination was more efficacious than erlotinib alone (P < 0.05). Similar results were detected by immunohistochemistry. Real-time quantitative PCR showed that YF, erlotinib, and the combination increased PTEN mRNA (P < 0.05, P < 0.01) compared with control, and the combination was more efficacious than erlotinib alone (P < 0.05). In conclusion, YF can regulate the main components of the PI3K/Akt pathway in TNBC xenografts. When YF was used in combination with erlotinib, it enhanced the antitumor effects of erlotinib on TNBC xenografts. These findings suggest that YF is suitable to use for the treatment of TNBC patients.

7.
Zhong Xi Yi Jie He Xue Bao ; 9(12): 1339-46, 2011 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-22152773

RESUMO

OBJECTIVE: To investigate the effects of Astragalus polysaccharide (APS) on proliferation of basal-like breast cancer cell line MDA-MB-468 cells and Akt phosphorylation in MDA-MB-468 cells. METHODS: APS at different concentrations was used to culture MDA-MB-468 cells for different time periods, and then proliferation of MDA-MB-468 cells was assayed using methyl thiazolyl tetrazolium (MTT) assay to determine the time- and dose-dependent effects of APS. For observing the effects of APS on phosphor-Akt (p-Akt), in-cell Western blot method was used after 1, 2, 4 and 7 d of culture in APS to detect protein expressions of p-Akt (Thr308) and p-Akt (Ser473). Protein levels of the key targets in p53/murine double minute 2 (MDM2) signaling pathway, such as p53, MDM2 and phosphatase and tensin homolog deleted on chromosome ten (PTEN) were also detected. After PTEN gene was silenced by small interfering RNA (siRNA) in MDA-MB-468 cells, expressions of p-Akt (Thr308 and Ser473) were assayed by the in-cell Western blot method after 2 d of APS treatment. RESULTS: APS at 1 and 0.5 mg/mL concentrations effectively inhibited the proliferation of MDA-MB-468 cells and was used in subsequent tests. Compared with the control group, APS decreased the protein expression of p-Akt (Thr308) in MDA-MB-468 cells after 1-, 2-, 4- and 7-day culture, and also decreased the protein expression of p-Akt (Ser473) and up-regulated the protein expression of MDM2 in MDA-MB-468 cells after 1- and 2-day culture. Expressions of p53 and PTEN were up-regulated after 7 d of APS culture. After silencing PTEN gene by siRNA, APS could not mediate Akt phosphorylation. CONCLUSION: APS can inhibit proliferation of basal-like breast cancer cell line MDA-MB-468, and down-regulate the expression of Akt phosphorylation. The antiproliferation mechanisms may be related to its effects of up-regulating the expressions of p53 and PTEN by regulating p53/MDM2 positive and negative feedback loops.


Assuntos
Astragalus propinquus/química , Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Polissacarídeos/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Antineoplásicos Fitogênicos/farmacologia , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Regulação para Baixo , Feminino , Humanos , PTEN Fosfo-Hidrolase/metabolismo , Fosforilação/efeitos dos fármacos , Polissacarídeos/isolamento & purificação , Proteína Supressora de Tumor p53/metabolismo
8.
Zhong Xi Yi Jie He Xue Bao ; 9(11): 1199-205, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22088585

RESUMO

BACKGROUND: As the main medicinal powder for drawing out pus and removing necrotic tissue in external therapies of traditional Chinese surgery, Sheng Powder has made great contributions to the treatment of inflammatory wounds and has the unique bactericidal and decay-discharging function that can not be replaced by antibiotics. However, Sheng Powder has toxicity because it contains mercury. So far, there is no clinical research on the standards of dose and usage of Sheng Powder and there is a lack of objective and quantitative criteria for operating standards and monitoring of toxicity and side effects. Therefore, the authors choose Jiuyi Powder, one of the most commonly used Sheng Powder, to evaluate the safety of its external use, and form a standardization program for clinical implementation. METHODS AND DESIGN: This study is a nonrandomized, nonblinded, self-controlled case study. There will be two key stages in the study. In stage one, 10 patients with plasma cell mastitis will be enrolled. The patients will receive continuous external application of Jiuyi Powder with a fixed dose (160 mg/d). Blood mercury and urine mercury levels will be dynamically tested at different time intervals, and the observation of pharmacokinetic parameters will be conducted after Jiuyi Powder has been absorbed by the surface of the wound. In stage two, 30 patients with each of the four conditions including postoperative wounds of head or facial surgeries, plasma cell mastitis, anal fistula and chronic absorption ulcer of the shank will be enrolled, respectively. According to the dose of 1.5 mg/cm(2), Jiuyi Powder will be externally applied to the wound surface for 14 d. On the basis of the first-phase study, the test time will be chosen and the toxicity outcome will be detected to evaluate the safety of external application of Jiuyi Powder and to establish recommendations for standardized clinical use. DISCUSSION: The purpose of this study is to evaluate the safety of the external application of Jiuyi Powder through a two-stage study. The pharmacokinetic parameters of external application of Jiuyi Powder at the clinical dose range, the changes in blood and urinary mercury levels and related safety indexes will be observed on different wounds located in different positions externally. The evaluation of whether Jiuyi Powder could be a routine medication for drawing out pus and removing necrotic tissue in inflammatory and refractory wounds will be stated. TRIAL REGISTRATION NUMBER: ChiCTR-TNC-11001366.


Assuntos
Ensaios Clínicos como Assunto , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/efeitos adversos , Fitoterapia/efeitos adversos , Fitoterapia/métodos , Adulto , Idoso , Feminino , Humanos , Masculino , Medicina Tradicional Chinesa/métodos , Mercúrio/sangue , Mercúrio/urina , Pessoa de Meia-Idade , Fitoterapia/normas , Projetos de Pesquisa , Cicatrização/efeitos dos fármacos , Adulto Jovem
9.
Org Lett ; 12(22): 5192-5, 2010 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-20973493

RESUMO

Bridging the triindole core and triarylboryl acceptor by an ethenylene linker at the 3,8,13- or 2,7,12-position, the resultant 3-BET and 2-BET show two-photon absorption (TPA) cross sections of δ = 2100 and 2500 GM (at 810 nm by femtosecond pulses in THF), respectively. The TPA enhancement of the 2,7,12-isomers is also found when comparing 3-BYT and 2-BYT (δ = 870 and 1900 GM) and 3-NET and 2-NET (36 and 400 GM).

10.
Acta Crystallogr C ; 66(Pt 4): m97-100, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20354296

RESUMO

In the title one-dimensional coordination polymer, {[Mn(C(5)O(5))(C(10)H(8)N(2))(H(2)O)].H(2)O}(n), each Mn(II) ion is seven-coordinated by four O atoms from two croconate ligands, two N atoms from a 2,2'-bipyridine (2,2'-bipy) ligand and one O atom from an aqua ligand. The croconate ligand bridges the Mn(II) ions in a bis-bidentate chelation mode, forming an extended [Mn(C(5)O(5))](n) chain running parallel to the [001] direction, with the lipophilic 2,2'-bipy ligands lying along one side and the hydrophilic water molecules along the opposite side. Coordinated water and solvent water molecules are arranged in the hydrophilic layer, which is characterized by O-H...O hydrogen bonds between croconate ligands. Meanwhile, 2,2'-bipy ligands from adjacent chains partially overlap and exhibit pi-pi interactions to form a lipophilic layer. The hydrophilic and lipophilic layers are arranged alternately to build a layer structure.


Assuntos
Manganês/química , Compostos Organometálicos/química , Polímeros/química , Cristalografia por Raios X , Ligações de Hidrogênio , Ligantes , Estrutura Molecular
11.
Zhong Xi Yi Jie He Xue Bao ; 7(12): 1174-80, 2009 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-20015441

RESUMO

OBJECTIVE: To observe the effects of Astragalus injection, astragaloside IV and formononetin on proliferation and Akt phosphorylation of basal-like human breast carcinoma cell lines MDA-MB-468 and MDA-MB-231. METHODS: The effects of different concentrations of Astragalus injection, astragaloside IV and formononetin on proliferation of breast cancer cell lines were assayed by methyl thiazolyl tetrazolium (MTT) assay, and their effects on phospho-Akt were assayed by in-cell Western blot method. RESULTS: The results of the MTT assay showed that the best concentrations of Astragalus injection, astragaloside IV, formononetin and astragaloside IV plus formononetin were 1 g/mL, 80 microg/mL, 40 microg/mL and 10 microg/mL plus 40 microg/mL respectively. After 1- or 2-day culture, Astragalus injection, astragaloside IV, formononetin and astragaloside IV plus formononetin decreased the expressions of p-Akt (Thr 308) and p-Akt (Ser 473) in MDA-MB-468 cells. Formononetin and astragaloside IV plus formononetin down-regulated the expression of p-Akt (Thr 308) protein in MDA-MB-231 cells after 1- and 2-day culture, but had no effects on the expression of p-Akt (Ser 473) protein in MDA-MB-231 cells. CONCLUSION: Astragalus injection, astragaloside IV and formononetin can inhibit proliferation of breast cancer cell lines MDA-MB-468 and MDA-MB-231, and the antiproliferation effects vary according to their concentrations. And the antiproliferation mechanisms may be related to their down-regulation effects on Akt phosphorylation.


Assuntos
Astrágalo (Planta) , Neoplasias da Mama/patologia , Proteína Oncogênica v-akt/efeitos dos fármacos , Fitoestrógenos/farmacologia , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Regulação para Baixo , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Isoflavonas/farmacologia , Proteína Oncogênica v-akt/metabolismo , Fosforilação/efeitos dos fármacos , Saponinas/farmacologia , Triterpenos/farmacologia
13.
Acta Crystallogr C ; 65(Pt 5): o198-201, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19407415

RESUMO

The title sulfur-rich organic molecular crystals, namely EDTO-TTP (C(9)H(4)OS(8)) and VDTO-TTP (C(9)H(2)OS(8)), are characterized by conjugated C-S bonds and S...S intermolecular short contacts. The planar EDTO-TTP molecules are parallel packed and exhibit strong intermolecular interactions, including side-by-side transverse S...S contacts, face-to-face longitudinal pi-pi interactions and C-H...O hydrogen bonding. On cooling the EDTO-TTP crystal from 220 to 120 K, the cell dimensions and the intermolecular distances (such as S...S contacts and especially pi-pi spacings) become shorter, while the intramolecular bonds become longer. The curved VDTO-TTP molecules are packed in such a way as to make the crystal fully depolarized. The intermolecular interactions of the VDTO-TTP crystal are relatively weak, because of the weak pi-pi interactions and the lack of hydrogen bonding.

14.
Zhong Xi Yi Jie He Xue Bao ; 6(10): 991-4, 2008 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18847530

RESUMO

The thread-dragging therapy, as a new external treatment of traditional Chinese medicine, could eliminate pathogenic factors and restore the vital qi by promoting qi and activating blood circulation to advance tissue repair in treating refractory sinus tracts or fistulae. This article reviewed its origin and development, and introduced its use in treating refractory sinus tracts or fistulae.


Assuntos
Medicina Tradicional Chinesa/métodos , Fístula Retal/terapia , Humanos
15.
Zhongguo Zhen Jiu ; 28(7): 499-502, 2008 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-18678162

RESUMO

OBJECTIVE: To compare differences of surface infrared radiation spectrums between Danzhong (CV 17) A high-sensitive PHE and non-acupoint control point in the patient of hyperplasia of mammary glands. METHODS: 201 surface infrared spectrograph was used to detect infrared radiation spectrums of Danzhong (CV 17) and non-acupoint control point at 1.5-16.0 microm wave band in the patient of hyperplasia of mammary glands. RESULTS: The shape of the infrared spectrums of Danzhong (CV 17) was similar to that of the non-acupoint control point, but with lower radiation intensity. Of the 59 wavelength spots detected, 13 (6.75-8.25 microm, 9.00 microm, 9.25 microm, 9.75 microm, 13.25-13.75 microm) had significant differences in infrared radiation intensity between Danzhong (CV 17) and non-acupoint control point (P<0.05). CONCLUSION: The intensity of infrared radition of Danzhong (CV 17) is lower and Qimen (LR 14) is higher than that of the non-acupoint control point in the patient with hyperplasia of mammary glands, showing that different channels are at different states of deficiency and excess under pathological condition of hyperplasia of mammary glands.


Assuntos
Pontos de Acupuntura , Mama/patologia , Raios Infravermelhos , Adulto , Feminino , Humanos , Hiperplasia , Medicina Tradicional Chinesa , Pessoa de Meia-Idade
16.
Ai Zheng ; 27(5): 555-8, 2008 May.
Artigo em Chinês | MEDLINE | ID: mdl-18479610

RESUMO

Breast cancer is the most common cancer in women all around the world. Presently, the prognosis of breast cancer is predicted based on histopathologic tumor characteristics. Molecular markers could help to predict the prognosis and treatment response in breast cancer. According to gene expression profile, invasive breast cancers are classified into four subtypes (luminal A, luminal B, HER2 overexpressing and basal-like) and associated with different prognosis. The basal-like subtype is associated with poor prognosis. This article simply introduced the distribution of molecular subtypes, summed up recent researches on the characteristics and prognosis of basal-like phenotype.


Assuntos
Neoplasias da Mama/metabolismo , Carcinoma Basocelular/metabolismo , Carcinoma Ductal de Mama/metabolismo , Queratina-17/metabolismo , Queratina-5/metabolismo , Neoplasias da Mama/classificação , Neoplasias da Mama/tratamento farmacológico , Carcinoma Basocelular/tratamento farmacológico , Carcinoma Ductal de Mama/tratamento farmacológico , Caveolina 1/metabolismo , Feminino , Humanos , Prognóstico , Receptor ErbB-2/metabolismo , Receptores Estrogênicos/metabolismo , Receptores de Progesterona/metabolismo
17.
Zhong Xi Yi Jie He Xue Bao ; 6(4): 399-404, 2008 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-18405609

RESUMO

OBJECTIVE: To investigate the effects of Astragalus injection (AI) on basal-like breast cancer cell line MDA-MB-468 and murine bone marrow stromal stem cells (mMSCs). METHODS: MDA-MB-468 cells and primary cultured mMSCs were treated by different concentrations of AI, and with untreated MDA-MB-468 cells as blank control. The morphology of cells was observed by phase-contrast inverted microscope and transmission electron microscopy. Cytotoxic effects of AI on MDA-MB-468 cells and mMSCs were evaluated by cell counting kit-8 (CCK-8) assay. Cell cycle and apoptosis of MDA-MB-468 cells induced by AI were measured by flow cytometry. Lactate dehydrogenase (LDH) activity in supernatants was measured by enzymatic colorimetric method. The expressions of epidermal growth factor receptor (EGFR) and p53 protein in MDA-MB-468 cells were evaluated by streptavidin-biotin-peroxidase complex method. RESULTS: A time-dependent cytotoxic effect of 1 g/ml AI was observed in MDA-MB-468 cells. 1 g/ml AI also had cytotoxic effect on mMSCs, but its effect was not better than cisplatin. 0.1 g/ml AI could promote the proliferation of mMSCs. Different concentrations of AI could all induce the apoptosis of MDA-MB-468 cells. There was no significant difference in LDH activity in the supernatants between blank control group and AI-treated and cisplatin-treated groups. AI could down-regulate the expressions of EGFR and p53 protein. CONCLUSION: The effects of AI on MDA-MB-468 cells and mMSCs are related to the concentration of AI, and its mechanism of inhibiting the proliferation of MDA-MB-468 cells may be due to down-regulation of the expressions of EGFR and p53 protein.


Assuntos
Astragalus propinquus/química , Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Células-Tronco/citologia , Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Células da Medula Óssea/citologia , Neoplasias da Mama/enzimologia , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Regulação para Baixo/efeitos dos fármacos , Receptores ErbB/metabolismo , Humanos , Proteína Supressora de Tumor p53/metabolismo
18.
Zhong Yao Cai ; 31(11): 1607-10, 2008 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-19260267

RESUMO

From late January to early February in 2008, Guangzhou suffered from the cold and humid rainy for 20 days, and the daily mean temperature was under 10 degrees C. In 794 species of medicinal plants in South China Botanical Garden, 78 species (9.8%) suffered from chilling injury. The reasons were analyzed and some useful suggestions were made.


Assuntos
Temperatura Baixa , Folhas de Planta/metabolismo , Plantas Medicinais/metabolismo , Aclimatação/fisiologia , China , Folhas de Planta/crescimento & desenvolvimento , Caules de Planta/crescimento & desenvolvimento , Caules de Planta/metabolismo , Plantas Medicinais/classificação , Plantas Medicinais/crescimento & desenvolvimento , Tempo (Meteorologia)
19.
J Tradit Chin Med ; 28(4): 293-8, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19226903

RESUMO

OBJECTIVE: To study the effect of Runing II (a Chinese herbal preparation for mammary cancer) on the growth and metastasis of transplanted tumor of mammary cancer MA-891-bearing TA2 mice and its mechanism. METHODS: The model of mammary cancer MA-891 cell strain transplanted tumor of TA2 mice with lung metastasis were developed to observe the effect of Runing II on the growth and metastasis of the transplanted tumor. The immunohistochemical method and image analysis were adopted to detect the levels of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor (VEGFR), and micro-vessel count (MVC) and micro-vessel area (MVA). RESULTS: In the Runing II group, the tumor weight inhibition rate and the lung metastasis inhibition rate were 37.3% and 65.4% respectively, the tumor growth and lung metastasis were obviously inhibited; And the levels of VEGF and VEGFR, MVC and MVA were significantly decreased as compared with those in the tumor-bearing control group (P<0.05). CONCLUSION: The Chinese herbal preparation Running II can inhibit the metastasis of tumor through inhibiting the angiogenesis, and the mechanism is possibly related with down-regulation of VEGF and VEGFR expression.


Assuntos
Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Mamárias Experimentais/tratamento farmacológico , Animais , Neoplasias da Mama/tratamento farmacológico , Feminino , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/fisiopatologia , Neoplasias Pulmonares/secundário , Neoplasias Mamárias Experimentais/patologia , Camundongos , Metástase Neoplásica/tratamento farmacológico , Transplante de Neoplasias , Distribuição Aleatória , Receptores de Fatores de Crescimento do Endotélio Vascular/metabolismo , Células Tumorais Cultivadas , Fator A de Crescimento do Endotélio Vascular/metabolismo
20.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 11): m1459, 2008 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-21580897

RESUMO

The title compound, [Ni(C(5)O(5))(C(10)H(8)N(2))(2)], lies across a crystallographic twofold axis, around which two 2,2'-bipyridine (2,2'-bipy) ligands are arranged in a propeller manner. The local geometry of the NiN(4)O(2) coordination core basically adopts an octa-hedral geometry. The mol-ecular twofold axis is along the direction of the mol-ecular dipole moment, and the complex is packed with its dipole moment alternately along the +b and -b directions. The crystal structure is stabilized by inter-molecular C-H⋯O hydrogen bonds.

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