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1.
Mikrochim Acta ; 186(8): 573, 2019 07 24.
Artigo em Inglês | MEDLINE | ID: mdl-31342189

RESUMO

An electrochemical sensor is described for simultaneous voltammetric determination of dopamine (DA) and uric acid (UA). It is based on the use of a nanomaterial composed of gold nanoparticles and 3-dimensional graphene (Au NP@3D GR). The 3D GR was prepared by chemical vapor deposition using nickel nanoparticles as the template at a temperature of around 900 °C. The surface of 3D GR contains oxygen-functional groups after treatment with acid. Carboxylated Au NP were self-assembled and anchored onto the surface of 3D GR. The nanomaterial was placed on a ITO electrode. The few-layer graphene on the ITO glass has a porous structure and the distribution of Au NP is uniform. The electrode shows a high sensitivity and a low detection limit for DA and UA. Figures of merit include detection limits of 0.1 M for DA and of 0.1 µM for UA, and well separated peaks at potentials of 0.18 and 0.30 V (vs. Ag/AgCl), respectively, at pH 7.0. The electrode has good repeatability and stability. Graphical abstract Carboxylated gold nanoparticles were self-assembled and immobilized on 3-dimensional graphene by chemical vapor deposition for simultaneous determination of dopamine (DA) and uric acid (UA).


Assuntos
Dopamina/análise , Técnicas Eletroquímicas/métodos , Grafite/química , Nanopartículas Metálicas/química , Ácido Úrico/análise , Animais , Técnicas Eletroquímicas/normas , Eletrodos/normas , Ouro/química , Humanos , Limite de Detecção , Reprodutibilidade dos Testes
2.
Mol Cancer ; 18(1): 101, 2019 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-31126310

RESUMO

Autophagy is a highly conserved catabolic process that mediates degradation of pernicious or dysfunctional cellular components, such as invasive pathogens, senescent proteins, and organelles. It can promote or suppress tumor development, so it is a "double-edged sword" in tumors that depends on the cell and tissue types and the stages of tumor. The epithelial-mesenchymal transition (EMT) is a complex biological trans-differentiation process that allows epithelial cells to transiently obtain mesenchymal features, including motility and metastatic potential. EMT is considered as an important contributor to the invasion and metastasis of cancers. Thus, clarifying the crosstalk between autophagy and EMT will provide novel targets for cancer therapy. It was reported that EMT-related signal pathways have an impact on autophagy; conversely, autophagy activation can suppress or strengthen EMT by regulating various signaling pathways. On one hand, autophagy activation provides energy and basic nutrients for EMT during metastatic spreading, which assists cells to survive in stressful environmental and intracellular conditions. On the other hand, autophagy, acting as a cancer-suppressive function, is inclined to hinder metastasis by selectively down-regulating critical transcription factors of EMT in the early phases. Therefore, the inhibition of EMT by autophagy inhibitors or activators might be a novel strategy that provides thought and enlightenment for the treatment of cancer. In this article, we discuss in detail the role of autophagy and EMT in the development of cancers, the regulatory mechanisms between autophagy and EMT, the effects of autophagy inhibition or activation on EMT, and the potential applications in anticancer therapy.


Assuntos
Antineoplásicos/farmacologia , Autofagia , Neoplasias/metabolismo , Antineoplásicos/uso terapêutico , Autofagia/efeitos dos fármacos , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Redes Reguladoras de Genes/efeitos dos fármacos , Humanos , Terapia de Alvo Molecular , Metástase Neoplásica , Neoplasias/tratamento farmacológico , Transdução de Sinais/efeitos dos fármacos
3.
Mol Cancer ; 18(1): 17, 2019 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-30678689

RESUMO

Autophagy is a genetically well-controlled cellular process that is tightly controlled by a set of core genes, including the family of autophagy-related genes (ATG). Autophagy is a "double-edged sword" in tumors. It can promote or suppress tumor development, which depends on the cell and tissue types and the stages of tumor. At present, tumor immunotherapy is a promising treatment strategy against tumors. Recent studies have shown that autophagy significantly controls immune responses by modulating the functions of immune cells and the production of cytokines. Conversely, some cytokines and immune cells have a great effect on the function of autophagy. Therapies aiming at autophagy to enhance the immune responses and anti-tumor effects of immunotherapy have become the prospective strategy, with enhanced antigen presentation and higher sensitivity to CTLs. However, the induction of autophagy may also benefit tumor cells escape from immune surveillance and result in intrinsic resistance against anti-tumor immunotherapy. Increasing studies have proven the optimal use of either ATG inducers or inhibitors can restrain tumor growth and progression by enhancing anti-tumor immune responses and overcoming the anti-tumor immune resistance in combination with several immunotherapeutic strategies, indicating that induction or inhibition of autophagy might show us a prospective therapeutic strategy when combined with immunotherapy. In this article, the possible mechanisms of autophagy regulating immune system, and the potential applications of autophagy in tumor immunotherapy will be discussed.


Assuntos
Autofagia/imunologia , Sistema Imunitário/imunologia , Neoplasias/imunologia , Neoplasias/terapia , Animais , Citocinas/imunologia , Humanos , Imunoterapia/métodos
4.
Mol Med Rep ; 18(3): 3137-3142, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30066945

RESUMO

The present study identified the cytotoxic effects of etomidate on the N2a neuroblastoma cell line. Etomidate induced apoptosis in N2a cells in a concentration­dependent manner, which was confirmed by western blotting and flow cytometry. Phase contrast microscopy was used to analyze the effect of etomidate on morphological characteristics. The number of the apoptotic cells was increased and confirmed by DAPI and PI staining, which served as a characteristic hallmark of apoptosis. Additionally, etomidate led to loss of mitochondrial membrane potential and resulted in the generation of reactive oxygen species in N2a cells. The western blot analysis revealed that N2a cells treated with etomidate had a significant modulation of pro­apoptotic proteins, includingpoly ADP­ribose polymerase (PARP), cleaved PARP, caspase­9 and procaspase­3. In conclusion, the present study determined that etomidate induced cytotoxic and apoptotic effects in N2a brain tumor cells in vitro.


Assuntos
Anestésicos/farmacologia , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Neoplasias Encefálicas/tratamento farmacológico , Etomidato/farmacologia , Neuroblastoma/tratamento farmacológico , Animais , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Camundongos , Neuroblastoma/metabolismo , Neuroblastoma/patologia , Espécies Reativas de Oxigênio/metabolismo
5.
J Biomed Res ; 32(4): 298-304, 2018 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-29884775

RESUMO

The study aimed to demonstrate the feasibility of an extradural nerve anastomosis technique for the restoration of a C5 and C6 avulsion of the brachial plexus. Nine fresh frozen human cadavers were used. The diameters, sizes, and locations of the extradural spinal nerve roots were observed. The lengths of the extradural spinal nerve roots and the distance between the neighboring nerve root outlets were measured and compared in the cervical segments. In the spinal canal, the ventral and dorsal roots were separated by the dura and arachnoid. The ventral and dorsal roots of C7 had sufficient lengths to anastomose those of C6. The ventral and dorsal of C4 had enough length to be transferred to those of C5, respectively. The feasibility of this extradural nerve anastomosis technique for restoring C5 and C6 avulsion of the brachial plexus in human cadavers was demonstrated in our anatomical study.

6.
J Biomed Res ; 32(2): 123-129, 2018 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-28866657

RESUMO

This study was aimed to introduce a novel entry point for pedicle screw fixation in the thoracic spine and compare it with the traditional entry point. A novel entry point was found with the aim of improving accuracy, safety and stability of pedicle screw technique based on anatomical structures of the spine. A total of 76 pieces of normal thoracic CT images at the transverse plane and the thoracic pedicle anatomy of 6 cadaveric specimens were recruited. Transverse pedicle angle (TPA), screw length, screw placement accuracy rate and axial pullout strength of the two different entry point groups were compared. There were significant differences in the TPA, screw length, and the screw placement accuracy rate between the two groups (P<0.05). The maximum axial pullout strength of the novel entry point group was slightly larger than that of the traditional group. However, the difference was not significant (P>0.05). The novel entry point significantly improved the accuracy, stability and safety of pedicle screw placement. With reference to the advantages above, the new entry point can be used for spinal internal fixations in the thoracic spine.

7.
Exp Ther Med ; 14(3): 2568-2576, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28962196

RESUMO

Mesenchymal stem cells (MSCs) are able to differentiate into hepatocytes, promote the regeneration of hepatic cells and inhibit the progression of hepatic fibrosis. Transforming growth factor (TGF)-ß1 is one of the key factors in the development of liver fibrosis, which also promotes extracellular matrix (ECM) formation. Drosophila mothers against decapentaplegic 7 (Smad7) is an essential negative regulator in the TGF-ß1/Smad signaling pathway. In the present study, bone mesenchymal stem cells (BMSCs) were isolated from rat bone marrow and transfected with lentiviral vectors carrying the Smad7 gene. Smad7-enhanced green fluorescent protein (EGFP)-BMSCs stably expressing Smad7 were subsequently co-cultured with hepatic stellate cells (HSCs) for 48 h. Smad7 and TGF-ß1 levels in the culture medium were detected using ELISA, and the levels of collagen (Col) I, Col III, laminin (LN) and hyaluronic acid (HA) were measured using immunoassays. The early apoptosis rates of HSCs were determined via flow cytometry. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to evaluate the mRNA and protein expression profiles, respectively. The results indicated that Smad7-EGFP-BMSCs stably expressing Smad7 were successfully constructed. Upon co-culturing with rat Smad7-EGFP-BMSCs, the early apoptotic rate of HSCs was significantly increased (P<0.05). Levels of Smad7 in the culture medium were also significantly increased (P<0.05), whereas the levels of TGF-ß1, Col I, Col III, LN and HA were significantly decreased (P<0.05). Furthermore, the mRNA and protein levels of Smad7 and matrix metalloproteinase 1 were significantly increased (P<0.05), whereas those of TGF-ß1, α-SMA, Smad2, smad3, TGF-ß receptor I, Col I, tissue inhibitors of metalloproteinase-1 and Col III were significantly decreased. The results of the present study suggest that rat BMSCs overexpressing Smad7 may inhibit the fibrosis of HSCs by regulating the TGF-ß1/Smad signaling pathway. This provides a novel insight into future treatments for liver fibrosis.

8.
Oncol Lett ; 12(5): 4061-4067, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27895772

RESUMO

Evidence is rapidly accumulating that long non-coding RNAs (lncRNAs) are involved in human tumorigenesis and are dysregulated in multiple cancers, including hepatocellular carcinoma (HCC). lncRNAs can regulate essential pathways that contribute to tumor initiation and progression with tissue specificity, which suggests that lncRNAs may be valuable biomarkers and therapeutic targets. HOX transcript antisense intergenic RNA (HOTAIR) has previously been demonstrated to be an oncogene and a negative prognostic factor in a variety of cancers; however, the factors that contribute to the upregulation of HOTAIR and the interaction between HOTAIR and microRNAs (miRNAs or miRs) are largely unknown. In the present study, the expression levels of HOTAIR, forkhead box C1 (FOXC1) and miRNA-1 were examined in 50 matched pairs of HCC and HCC cells. The effects of HOTAIR on HCC cell proliferation were tested using trypan blue exclusion assay. The effect of HOTAIR on HCC growth in vivo was determined in a (nu/nu) mouse model. A computational screening of HOTAIR promoter was conducted to search for transcription factor-binding sites. FOXC1 binding to the promoter region of HOTAIR was confirmed using a chromatin immunoprecipitation assay. A search for miRNAs that had complementary base paring with HOTAIR was performed utilizing an online software program. The interaction between miR-1 and HOTAIR was examined using a luciferase reporter assay. Gain and loss of function approaches were used to determine the changes of HOTAIR or miR-1 expression. The relative levels of FOXC1 and HOTAIR expression in HCC tissues and HepG2 cells were significantly higher than those in normal liver LO2 cells and adjacent carcinoma tissues; the relative expression of miR-1 exhibited the opposite pattern. Overexpression of HOTAIR promoted HCC cell proliferation and progression of tumor xenografts. The present authors have demonstrated that FOXC1 binds to the upstream region of HOTAIR in HCC cells and that FOXC1 activates lncRNA HOTAIR expression in HCC HepG2 cells, which suggests that HOTAIR harbors a miRNA-1 binding site. The present data revealed that this binding site is vital for the regulation of miRNA-1 by HOTAIR. Furthermore, HOTAIR negatively regulated the expression of miRNA-1 in HepG2 cells. Additionally, the present study demonstrated that the oncogenic activity of HOTAIR is in part based on the negative regulation of miR-1. Taken together, these results suggest that HOTAIR is a FOXC1-activated driver of malignancy, which acts in part through the repression of miR-1.

9.
Exp Ther Med ; 12(3): 1934-1938, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27602099

RESUMO

Tacrolimus (TAC) has been shown to improve remission from proteinuria in patients with refractory IgA nephropathy (IgAN); however, the efficacy and safety of TAC in such patients have not been fully explored. Therefore, the present study was conducted to evaluate the tolerance to and efficacy of TAC combined with low-dose corticosteroids in patients with refractory IgAN. This was a single-center retrospective study. A total of 28 patients with refractory IgAN were randomly included and received TAC plus corticosteroid; 26 patients received TAC and prednisone, and 2 patients received TAC and methylprednisolone. In addition, all patients were treated with an angiotensin inhibitor. Total urinary protein excretion, serum albumin, blood glucose, complete remission (CR), partial remission (PR), cholesterol, low-density lipoprotein (LDL), serum creatinine (Scr) and estimated GFR (eGFR) were tested at baseline and at 3, 6 and 12 months after the initiation of treatment in all patients. The primary endpoints were CR and PR. Secondary endpoints included changes of Scr, eGFR, clinical data and adverse events. After 12 months, CR was achieved in 40.1% of patients and PR in 43.4%, yielding a total response rate of 83.5%, and the total urinary protein excretion, serum albumin, cholesterol and LDL results were improved significantly compared with those at baseline. Proteinuria and serum albumin results were significantly improved by month 3 of treatment. Two patients relapsed during months 3-6 of follow-up. At the 12-month follow-up, renal function was improved compared with the baseline level as evidenced by eGFR and Scr, respectively. The blood glucose level was stable. One case of pneumococcal pneumonia developed in a patient treated with TAC plus low-dose methylprednisolone and one case of upper gastrointestinal hemorrhage was found in a patient treated with TAC plus low-dose prednisone; both cases completely recovered after treatment. In conclusion, TAC combined with low-dose corticosteroids may be an effective and safe therapeutic option for the treatment of refractory IgAN. However, given the small number of patients in this study, further prospective randomized controlled trials are required with a larger sample of participants and longer follow-up period.

10.
Beijing Da Xue Xue Bao Yi Xue Ban ; 48(1): 89-93, 2016 Feb 18.
Artigo em Chinês | MEDLINE | ID: mdl-26885915

RESUMO

OBJECTIVE: To evaluate the vital signs changes, influence factors in different grades of hypertension patients during the treatment of acute pulpitis, in order to obtain the risk prevention measures. METHODS: In this study, 90 different grades of hypertension patients with acute pulpitis were recruited from February 2014 to February 2015 in the Department of Oral Emergency, Peking University School and Hospital of Stomatology. The information about the patients'general health, oral treatment, life signs of change information was collected. Patients were divided into high risk group, middle risk group, and low risk group (30 patients for each group). RESULTS: (1) Compared with the preoperative, systolic blood pressure (90%), diastolic blood pressure (80%), heart rate increase (100%) were increased in the high risk group. The increase rates of the middle risk group and the low risk group were significantly lower than those of the high risk group (P<0.01). At the same time, the systolic blood pressure of 1/4 (26.7%) patients in high risk group increased more than 20 mmHg (1 mmHg=0.133 kPa), and the diastolic blood pressure of 2/5 patients in high risk group increased more than 10 mmHg, the difference was statistically significant compared with the other two groups (P<0.05). (2) Compared with the preoperative, the average increase of the maximum peak were increased [systolic blood pressure (18.0 ± 1.5) mmHg, diastolic blood pressure (8.0 ± 1.7) mmHg], the mean of heart rate changes [(7.0 ± 0.3) beats per minute] was also increased in the high risk group, while these two indicators were decreased in the low risk group and the middle risk group. The electrocardiogram (ECG) was changed in 6 cases during the treatment in the high risk group. No significantly changed were observed in the low risk group and the middle risk group. (3) Compared the risk assessment in preoperative with that in postoperative, in the middle risk group, 23 cases were evaluated as medium risk in final evaluation, 6 as low risk, and 1 as high risk (risk assessment increased); in the high risk group, 20 cases were evaluated as high risk, 7 as very high risk, and 3 as medium risk (risk assessment decreased). CONCLUSION: Oral treatment is very safe for patients with hypertension, but the risk factor, target organ damage, and complications will also increase the risk of cardiovascular events in elderly patients during the acute pulpitis treatment. Dentist should take some measures to avoid the risks.


Assuntos
Hipertensão/classificação , Pulpite/complicações , Pressão Sanguínea , Humanos , Pulpite/terapia , Medição de Risco
11.
Springerplus ; 5(1): 2107, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-28066696

RESUMO

This study intends to investigate the correlations of miR-124a and miR-30d with clinicopathological features of breast cancer (BC) patients with type 2 diabetes mellitus (T2DM). A total of 72 BC patients with T2DM (diabetic group) and 144 BC patients without T2DM (non-diabetic group) were enrolled in this study. Blood glucose was detected by glucose oxidase methods. Glycosylated hemoglobin (HbA1c) was measured by high performance liquid chromatography. Fasting insulin (FIns) was measured by chemiluminescent microparticle immunoassay. Automatic biochemical analyzer was used to detect triglyceride, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). Estradiol (E2) was detected by radioimmunoassay. Homeostasis model assessment was applied to assess the insulin resistance (HOMA-IR) and ß-cell insulin secretion (HOMA-IS). The expressions of miR124a and miR-30d were measured by quantitative real-time polymerase chain reaction (qRT-PCR). There were significant differences in age, the ratio of menopause, body mass index (BMI), HDL-C, TC, 2-h plasma glucose (2hPG), FIns, HbA1c, HOMA-IS and HOMA-IR between the diabetic and non-diabetic groups. The diabetic group had higher incidence of lymph node metastasis than non-diabetic group. The miR-124a expression was down-regulated while the miR-30d expression was up-regulated in BC patients with T2DM. The correlation analysis showed that miR-124a expression was positively correlated with HDL-C, while it was negatively correlated with age, HbA1c, LDL-C and E2. However, the miR-30d expression was negatively correlated with HDL-C but positively correlated with age, HbA1c, LDL-C and E2. In conclusion, miR-124a and miR-30d may be correlated with clinicopathological features of BC patients with T2DM. The miR-124a and miR-30d could serve as novel biomarkers for early diagnosis of BC in patients with T2DM.

12.
Environ Sci Pollut Res Int ; 23(4): 3385-98, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26490913

RESUMO

Samples were collected monthly from January to December in 2010, and daily observations were made during the water-sediment regulation event in June-July 2010. Sequential extractions were applied to determine the forms of P in different particle-size fractions and to assess the potential bioavailability of particulate phosphorus (PP). The results indicated that exchangeable phosphorus, organic phosphorus, authigenic phosphorus, and refractory phosphorus increased with the decreasing of particulate size; conversely, detrital phosphorus decreased with the decreasing of particulate size. The content of bioavailable particulate phosphorus (BAPP) varied greatly in different sizes of particles. In general, the smaller the particle size, the higher the content of bioavailable phosphorus and its proportion in total phosphorous was found in these particles. Hydrological forcing controlled the variability in the major P phases found in the suspended sediments via changes in the sources and the particle grain-size distribution. The variation of particle sizes can be attributed also to different total suspended sediment (TSS) sources. Water-sediment regulation (WSR) mobilized only particulate matter from the riverbed, while during the rainstorm soil erosion and runoff were the main source. The BAPP fluxes associated with the "truly suspended" fraction was approximately 200 times larger than the dissolved inorganic phosphorus (DIP) flux. Thus, the transfer of fine particles to the open sea is most probably accompanied by BAPP release to the DIP and can support greater primary and secondary production.


Assuntos
Fósforo/química , Rios/química , Sedimentos Geológicos/química , Hidrologia , Tamanho da Partícula , Material Particulado/química , Movimentos da Água
13.
Kaohsiung J Med Sci ; 31(1): 42-6, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25600919

RESUMO

The tolerance of mycophenolate mofetil (MMF; Shanghai Roche, China) in Lee Classes III, IV, and V immunoglobulin A nephropathy (IgAN) remains unclear. This article reports nine cases of severe pneumonia (SP), including pneumocystis pneumonia (PCP) and cytomegalovirus (CMV) pneumonia, and its risk factors in MMF plus low-dose corticosteroid-treated patients with Lee Classes III, IV, and V IgAN. Fifty-three patients with IgAN were included in this single-center study. The treatment regimen was MMF (1-1.5 g/d) plus low-dose corticosteroids (0.5 mg/kg/d). SP was defined as diffuse bilateral lung infiltrate with respiratory failure. PCP was diagnosed by detecting the organisms in the sputum and bronchoalveolar lavage. CMV infection was diagnosed through serum screening for CMV-IgG and IgM antibodies and CMV-DNA testing by a real-time polymerase chain reaction assay. The risk factors of SP were analyzed. Nine cases (16.9%) of SP occurred in this study. All SP developed at approximately the 10(th)-14(th) week after the initiation of the regimen: PCP was diagnosed in four cases and CMV infection in two cases. Renal function impairing was more serious in patients with SP than in those without SP, as evidenced by estimated glomerular filtration rate (p = 0.019) and serum creatinine level (p = 0.016). Six of the nine SPs occurred in MMP plus low-dose methylprednisolone group, which was statistically higher than that in the MMF plus low-dose prednisone group (p = 0.000). The incidence of SP in this study was 16.9%. Chronically impaired renal function and the use of methylprednisolone may be the risk factors for SP.


Assuntos
Corticosteroides/uso terapêutico , Glomerulonefrite por IGA/tratamento farmacológico , Ácido Micofenólico/análogos & derivados , Pneumonia/tratamento farmacológico , Corticosteroides/efeitos adversos , Adulto , Feminino , Glomerulonefrite por IGA/metabolismo , Humanos , Imunoglobulina A/metabolismo , Masculino , Metilprednisolona/efeitos adversos , Metilprednisolona/uso terapêutico , Pessoa de Meia-Idade , Ácido Micofenólico/efeitos adversos , Ácido Micofenólico/uso terapêutico , Pneumonia por Pneumocystis/tratamento farmacológico , Prednisona/uso terapêutico , Estudos Retrospectivos , Fatores de Risco
14.
Yao Xue Xue Bao ; 49(8): 1181-7, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25322562

RESUMO

To investigate theological properties of common hydrophilic gel excipients such as Carbopol based on viscosity, the viscosity was determined by rotation method and falling-ball method. Linear regression was made between ln(eta) and concentration, the slope of which was used to explore the relation between viscosity and concentration of different excipients. The viscosity flow active energy (E(eta)) was calculated according to Arrhenius equation and was used to investigate the relation between viscosity and temperature of different excipients. The results showed that viscosities measured by two methods were consistent. Concentration of guargum (GG) and hydroxypropylmethyl cellulose (HPMC) solution had a great influence on the viscosity, k > 5; while concentration of polyvinylpyrrolidone-K30 (PVP-K30) and polyethylene glycol 6000 (PEG6000) exerted a less effect on viscosity, k < 0.2; viscosity flow active energy of different excipients were close, which ranged from 30 to 40 kJ x mol(-1). Therefore, theological properties study could provide the basis for application of excipients and establish a foundation for the research of relation between excipients structure, property and function.


Assuntos
Excipientes/química , Géis/química , Reologia , Polietilenoglicóis/química , Polivinil/química , Povidona/química , Temperatura , Viscosidade
15.
Huan Jing Ke Xue ; 35(3): 942-50, 2014 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-24881381

RESUMO

Surface sediments were collected from Yellow River estuary wetland. The distribution of phosphorus in sediments was analyzed with modified SEDEX. The results indicated that the contents of TP in surface sediments varied from 12.12 micromol x g(-1) to 25.37 micromol x g(-1), and the mean value was 20.70 micromol x g(-1), in which the Detrital P and Authigenic P were the main forms. Median particle size was closely related with the distribution of phosphors, Exchangeable P, Authigenic P and Organic P mainly consisted of smaller sediment size, while Detrital P mainly consisted of larger sediment size. The distribution of P in sediment was affected by organic matter. Exchangeable P, organic P and refractory P increased with the increasing TOC. The bio-available phosphorus included exchangeable P, iron-bound P,organic P and ranged from 1.15-6.74 micromol x g(-1), with an average of 4.27 micromol x g(-1) for all sediment samples. The contribution of BAP to TP was 6.35% -30.4%.


Assuntos
Monitoramento Ambiental , Estuários , Sedimentos Geológicos/análise , Fósforo/análise , China , Tamanho da Partícula , Rios , Áreas Alagadas
16.
Huan Jing Ke Xue ; 33(10): 3361-7, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23233960

RESUMO

Surface sediments were collected from the North Yellow Sea in October 2007. The sediments were analyzed with modified SEDEX to obtain six forms of phosphorus. The results indicate that among the total phosphorus the detrital phosphorus and authigenic phosphorus are the main forms and the content varies from 25.7 to 122.5 microg x g(-1) and from 5.7 to 176.2 microg x g(-1), respectively, which taking up 17.0% -56.9% and 10.2% -49.8% of the total phosphorus. The content of exchangeable phosphorus, extractable organic phosphorus, iron-bound phosphorus and refractory phosphorus ranges from 5.5 to 43.9, from 4.1 to 41.4, from 2.3 to 26.7, from 6.0 to 33.6 microg x g(-1), respectively. Detrital and authigenic phosphorus are the major forms of the total phosphorus, indicating that the natural input is the major source of particle phosphorus in the study area. The content of iron-bound phosphorus is low, indicating that human input is minor in the area; mass fraction of the extractable organic phosphorus and refractory organic phosphorus is low, indicating that there is a low organic content while the main form of the total phosphorus is inorganic in the surface sediments of this area. Bioavailable phosphorus as exchangeable phosphorus, extractable organic phosphorus and iron-bound phosphorus account for 6.7% - 33.2% of the total particle phosphorus, indicating that bioavailability of phosphorus in the surface sediments is low.


Assuntos
Monitoramento Ambiental , Sedimentos Geológicos/química , Compostos de Fósforo/análise , Fósforo/química , China , Oceanos e Mares , Tamanho da Partícula , Fósforo/análise , Compostos de Fósforo/química
17.
Huan Jing Ke Xue ; 33(10): 3457-65, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23233973

RESUMO

Abstract: Surface soil samples from the Yellow River Estuary Wetlands were analyzed for total organic carbon (TOC) and n-alkanes. Molecular indicators of n-alkanes were calculated and their potential as organic matter source indicators was discussed and compared among different sampling areas and times. C25-C33 n-alkanes with odd-to-even predominance were observed in most surface soils of the wetlands, suggesting the dominant contribution of terrestrial higher plants for the soil organic matter (SOM), and the ubiquitous presence of unresolved complex mixture indicated the presence of petroleum contamination. Total n-alkane concentrations in soils varied from 0.57 microg x g(-1) to 3.90 microg x g(-1), and distinct spatial and temporal differences were observed. In April 2009 (dry season), total n-alkane concentration was higher than that in June 2009 (during water-sediment regulation) with the maximum concentration observed at the core area of the wetlands, followed by the north side of the Yellow River after the last pontoon bridge, and the abandoned channel area of the Yellow River. The opposite trend of total n-alkane concentration was observed in June. The variation of total n-alkane concentration at two sampling time points were positively correlated with TOC and negatively correlated with sediment grain size, suggesting the influence of hydro-environment on the accumulation of soil organic matter. Molecular indicators of n-alkanes, such as average chain length (ACL), odd-even predominance (OEP), alkane index (AI), carbon preference index (CPI) and Terrigenous/ Aquatic Ratio (TAR) indicated that the maturity of organic matter in soils of the wetlands was low, and the dominant source of the SOM was terrestrial higher plants and mainly herbaceous plants. Compared with other indicators, TAR is better in reflecting the variation of hydro-environment.


Assuntos
Alcanos/química , Ecossistema , Estuários , Poluentes do Solo/análise , Áreas Alagadas , Alcanos/análise , Carbono/análise , China , Compostos Orgânicos/análise , Rios/química , Solo/química
18.
Asian Pac J Trop Med ; 5(6): 472-5, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22575981

RESUMO

OBJECTIVE: To appraise the effectiveness of HbA(1c) and fasting plasma glucose (FPG) on screening diabetes in health check-up. METHODS: A total of 1,337 individuals (male 850, female 487), aged 27 to 91 years with HbA(1c) test were included. Participates with HbA(1c) ≥6.0% or FPG≥6.1 mmol/L underwent oral glucose tolerance test (OGTT). Diabetes mellitus was diagnosed according to the criteria of WHO in 1999, FPG≥7.0 mmol/L and/or OGTT 2 h-postload plasm glucose (2 h-PG)≥11.1 mmol/L. The sensitivity and specificity of HbA(1c) thresholds and FPG or combination test on screening of diabetes were analyzed. RESULTS: A total of 842 subjects had HbA(1c) <6.0%, in which 32 had isolated FPG≥6.1 mmol/L, of 495 had HbA(1c)≥6.0%. Subjects with HbA(1c)≥6.0% had significant increased disorder indexes than those with HbA(1c)<6.0%. 527 subjects who had HbA(1c)≥6.0% or FPG≥6.1 mmol/L underwent OGTT. A total of 234 subjects were newly diagnosed diabetes, including 123 (123/234, 52.56%) with FPG≥7.0 mmol/L, and 111 subjects (111/234, 47.43%) with isolated 2 h-PG≥11.1 mmol/L. Among 234 new diabetes, 91.88% (215 subjects) had HbA(1c)≥6.3%, and 77.40% (181 subjects) had HbA(1c)≥6.5%. HbA(1c)≥6.3% combined FPG ≥7.0 mmol/L increased the positive rate of newly diagnosed diabetes from 91.88% to 96.58%. CONCLUSIONS: HbA(1c) is a practical and convenient tool for screening undiagnosed diabetes in routine health check-up of a large population. Combined use of HbA(1c)≥6.3% and/or FPG≥7.0 mmol/L is efficient for early detection of diabetes.


Assuntos
Glicemia/metabolismo , Diabetes Mellitus Tipo 2/diagnóstico , Hemoglobina A Glicada/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Diabetes Mellitus Tipo 2/sangue , Diagnóstico Precoce , Jejum/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Exame Físico/métodos
19.
Huan Jing Ke Xue ; 32(2): 368-74, 2011 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-21528556

RESUMO

Suspended particulate matter samples were collected from Lijin Station on the Yellow River during the 2008 water and sediment regulation. Using water elutriation method, samples were physically separated according to grain size, and phosphorus forms were detected by the improved SEDEX technology. Results show that authigenic apatite phosphorus and organic phosphorus are the dominant forms of the particulate phosphorus. During water and sediment regulation, concentrations of total particulate phosphorus, loosely adsorbed phosphorus, organic phosphorus and refractory phosphorus are lower than those in other time of the year, while the concentration of detrital fluorapatite phosphorus is higher than that in other time. Grain size is the primary variable in the determination of the content of various particulate phosphorus forms. In fine particles, loosely adsorbed phosphorus, organic phosphorus and refractory phosphorus concentrations are high, while detrital fluorapatite phosphorus concentration is high in coarse particles. About 28.3% of the total particulate phosphorus is in the form of bio-available phosphorus, of which organic phosphorus is the major component. During the water and sediment regulation, the fluxes of sediment, particulate phosphorus and particulate bio-available phosphorus are 6.63 x 10(7) t, 3.42 x 10(4) t, and 8.30 x 10(3) t, which are 83.2%, 78.6%, and 80.2% each of the total fluxes in 2008.


Assuntos
Monitoramento Ambiental/métodos , Sedimentos Geológicos/química , Fósforo/química , Movimentos da Água , China , Ecossistema , Tamanho da Partícula , Fósforo/análise , Rios/química
20.
Zhonghua Wei Chang Wai Ke Za Zhi ; 14(2): 140-2, 2011 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-21365510

RESUMO

OBJECTIVE: To evaluate the association between survival and expressions of epidermal growth factor receptor(EGFR) and protein kinase B(AKT) in gastric carcinoma specimens. METHODS: EGFR and AKT were detected by immunohistochemistry in 84 patients with gastric cancer and 15 controls. Association between their expressions and clinical characteristics as well as prognosis were analyzed. RESULTS: In patients with gastric cancer,expression rates were significantly higher than those in 15 normal gastric tissues for EGFR(55.9% vs. 20%, P<0.05) and AKT(64.3% vs. 6.7%, P<0.01). Expression of EGFR was associated with late staging(P<0.01), while expression of AKT was associated with lymph node metastasis(P<0.01). Overall 5-year survival rate was significantly higher in patients with EGFR negative expression (49.0% vs. 22.0%, P<0.05). Overall 5-year survival rate was significantly higher in patients with AKT expression(45.7% vs. 30.2%, P<0.05). CONCLUSION: Expression of EGFR and AKT negative may be used to evaluate the degree of differentiation and prognosis in patients with gastric carcinoma.


Assuntos
Receptores ErbB/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Neoplasias Gástricas/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Distribuição de Qui-Quadrado , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Prognóstico , Neoplasias Gástricas/patologia , Adulto Jovem
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