Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 453
Filtrar
1.
Drug Des Devel Ther ; 15: 679-688, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33628013

RESUMO

Background: The application of random pattern skin flaps is limited in plastic surgery reconstruction due to necrosis. Trans-cinnamaldehyde has antibacterial, anticancer, and antioxidant properties. In this study, we aimed to investigate the effect of trans-cinnamaldehyde on skin flap survival and its possible mechanism regarding nitric oxide. Materials and Methods: One hundred forty male Sprague-Dawley rats were randomly divided into seven groups (n = 20 each group). After the dorsal flap was raised, different doses of trans-cinnamaldehyde (10, 20, and 30 mg/kg) were immediately given by oral gavage in the three different groups. To assess the possible involvement of the nitric oxide system, NG-nitro-L-arginine methyl ester (L-NAME, a nonselective nitric oxide synthase inhibitor) was used in this study. All flap samples were incised on postoperative day 7. Results: Our results showed that flap survival was increased significantly in the 20 mg/kg (P < 0.001) trans-cinnamaldehyde (TC) group compared to the control group or 30 mg/kg TC group. This protective function was restrained by coadministration of L-NAME with 20 mg/kg TC. The results of histopathology, laser Doppler, arteriography mediated with oxide-gelatine, and fluorescent staining all showed a significant increase in capillary count, collagen deposition, angiogenesis, and flap perfusion. Immunohistochemistry results revealed a significant increase in the expression of CD34, eNOS, and VEGF. Conclusion: Trans-cinnamaldehyde increased flap survival through the nitric oxide synthase pathway and contributed to angiogenesis. A concentration of 20 mg/kg trans-cinnamaldehyde was recommended in this study.

2.
Am J Hypertens ; 34(1): 121-122, 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33599748

RESUMO

BACKGROUND: To investigate the function of transient receptor potential melastatin 2 (TRPM2) in vascular reactivity induced by 5-hydroxytryptamine (5-HT) in the aorta during development of atherosclerosis in mice. METHODS: Forty mice were randomly divided into 4 groups: C57BL/6J on normal diet (C57 + ND), C57BL/6J on high-fat diet (C57 + HFD), apolipoprotein E gene knockout mice (ApoE-/-) on ND (ApoE-/- + ND), and ApoE-/- on HFD (ApoE-/- + HFD). They were fed with a ND or HFD for 16 weeks. Aortic TRPM2 expression and isometric contractions were analyzed. RESULTS: In the ApoE-/- + HFD group, body weight, blood glucose, and blood lipid concentrations were increased, and aortic plaques were developed. Compared with the other 3 groups, aortic TRPM2 mRNA and protein levels were significantly increased in the ApoE-/- + HFD group (P < 0.01). Aortic reactivity to 5-HT was enhanced in ApoE-/- + HFD mice with lower EC50 values. The enhanced reactivity to 5-HT was significantly inhibited by TRPM2 inhibitors, N-p-amylcinnamoyl anthranilic acid (1 µmol/l) and 2-aminoethyl diphenylborinate (10 µmol/l). CONCLUSIONS: Aortic TRPM2 expression is upregulated in ApoE knockout mice fed with a HFD. Upregulation of TRPM2 enhances 5-HT vascular reactivity during development of atherosclerosis.

3.
Cell Death Dis ; 12(2): 202, 2021 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-33608512

RESUMO

Ring1b is a core subunit of polycomb repressive complex 1 (PRC1) and is essential in several high-risk cancers. However, the epigenetic mechanism of Ring1b underlying breast cancer malignancy is poorly understood. In this study, we showed increased expression of Ring1b promoted metastasis by weakening cell-cell adhesions of breast cancer cells. We confirmed that Ring1b could downregulate E-cadherin and contributed to an epigenetic rewiring via PRC1-dependent function by forming distinct complexes with DEAD-box RNA helicases (DDXs) or epithelial-mesenchymal transition transcription factors (EMT TFs) on site-specific loci of E-cadherin promoter. DDXs-Ring1b complexes moderately inhibited E-cadherin, which resulted in an early hybrid EMT state of epithelial cells, and EMT TFs-Ring1b complexes cooperated with DDXs-Ring1b complexes to further repress E-cadherin in mesenchymal-like cancer cells. Clinically, high expression of Ring1b with DDXs or EMT TFs predicted low levels of E-cadherin, metastatic behavior, and poor prognosis. These findings provide an epigenetic regulation mechanism of Ring1b complexes in E-cadherin expression. Ring1b complexes may be potential therapeutic targets and biomarkers for diagnosis and prognosis in invasion breast cancer.

4.
Bioengineered ; 12(1): 516-526, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33550896

RESUMO

In order to isolated and identified the bacterial strains from wheat rhizosphere and evaluated the effect of different concentration of bacterial fermentation broth on the wild oats weed growth. This experiment carried out the separation and purification of dominant bacterial strains from the wheat rhizosphere soil, and performed the fermentation broth biological activity assessment by measured the seed germination and plant growth from 20 wheat varieties. The results had shown that the bacterial fermentation broth inhibits the growth of wild oat seedlings and plants to varying degrees, bacterial strains of X3, X4, X8, X12, X16 and X20 has certain level of inhibition activity and X20 has the highest herbicidal effectiveness. According to molecular biology identification, obtained superior bacterial strains X20 was Bacillus as potentially inhibitor for developing of bacterial-based bioherbicides for wild oats weed control management in the wheat field.

5.
Inorg Chem ; 2021 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-33577307

RESUMO

Trace doping is an efficient way to improve the stability of nickel-rich layered cathodes for lithium-ion batteries, but the structural origin of such improvement, rather than a simple replacement, has been rarely explored. Herein, Ga dopants have been introduced into a nickel-rich host, LiNi0.9Co0.1O2, by a combination of coprecipitation and the solid-state sintering method. Structural analyses based on high-resolution synchrotron powder diffraction data and X-ray absorption spectra suggest that Ga preferentially sits in the NiO6 slabs, resulting in reduced Ni/Li cationic mixing and depressed lattice vibration. Due to the smaller dissociation energy of Ga-O bonds, some Ga3+ cations migrate first toward Li layers upon delithiation and form the GaO4 tetrahedral symmetry irreversibly during the electrochemical process, which acts as a pillar in the subsequent electrochemical processes. As a result, the doped material exhibits both improved cycling performance and rate capability under a high operating voltage (4.5 V) due to the stabilized structure in the lithiation/delithiation process. This study illustrates how a dopant enhances the electrochemical stability in views of both pristine and charged structure and suggests that a positive effect is expected from the dopant favoring the tetrahedral symmetry (e.g., Al).

6.
J Integr Med ; 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-33589406

RESUMO

BACKGROUND: Shenyankangfu Tablet (SYKFT) is a Chinese patent medicine that has been used widely to decrease proteinuria and the progression of chronic kidney disease. OBJECTIVE: This trial compared the efficacy and safety of SYKFT, for the control of proteinuria in primary glomerulonephritis patients, against the standard drug, losartan potassium. DESIGN, SETTING, PARTICIPANTS AND INTERVENTION: This was a multicenter, double-blind, randomized, controlled clinical trial. Primary glomerulonephritis patients, aged 18-70 years, with blood pressure ≤ 140/90 mmHg, estimated glomerular filtration rate (eGFR) ≥ 45 mL/min per 1.73 m2, and 24-hour proteinuria level of 0.5-3.0 g, were recruited in 41 hospitals across 19 provinces in China and were randomly divided into five groups: SYKFT, losartan potassium 50 mg or 100 mg, SYKFT plus losartan potassium 50 mg or 100 mg. MAIN OUTCOME MEASURES: The primary outcome was change in the 24-hour proteinuria level, after 48 weeks of treatment. RESULTS: A total of 735 participants were enrolled. The percent decline of urine protein quantification in the SYKFT group after 48 weeks was 8.78% ± 2.56% (P = 0.006) more than that in the losartan 50 mg group, which was 0.51% ± 2.54% (P = 1.000) less than that in the losartan 100 mg group. Compared with the losartan potassium 50 mg group, the SYKFT plus losartan potassium 50 mg group had a 13.39% ± 2.49% (P < 0.001) greater reduction in urine protein level. Compared with the losartan potassium 100 mg group, the SYKFT plus losartan potassium 100 mg group had a 9.77% ± 2.52% (P = 0.001) greater reduction in urine protein. With a superiority threshold of 15%, neither was statistically significant. eGFR, serum creatinine and serum albumin from the baseline did not change statistically significant. The average change in TCM syndrome score between the patients who took SYKFT (-3.00 [-6.00, -2.00]) and who did not take SYKFT (-2.00 [-5.00, 0]) was statistically significant (P = 0.003). No obvious adverse reactions were observed in any group. CONCLUSION: SYKFT decreased the proteinuria and improved the TCM syndrome scores of primary glomerulonephritis patients, with no change in the rate of decrease in the eGFR. SYKFT plus losartan potassium therapy decreased proteinuria more than losartan potassium therapy alone. TRIAL REGISTRATION NUMBER: NCT02063100 on ClinicalTrials.gov.

7.
Sci China Life Sci ; 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33420923

RESUMO

African trypanosomatid parasites escape host acquired immune responses through periodic antigenic variation of their surface coat. In this study, we describe a mechanism by which the parasites counteract innate immune responses. Two TatD DNases were identified in each of Trypanosoma evansi and Trypanosoma brucei. These DNases are bivalent metal-dependent endonucleases localized in the cytoplasm and flagella of the parasites that can also be secreted by the parasites. These enzymes possess conserved functional domains and have efficient DNA hydrolysis activity. Host neutrophil extracellular traps (NETs) induced by the parasites could be hydrolyzed by native and recombinant TatD DNases. NET disruption was prevented, and the survival rate of parasites was decreased, in the presence of the DNase inhibitor aurintricarboxylic acid. These data suggest that trypanosomes can counteract host innate immune responses by active secretion of TatD DNases to degrade NETs.

8.
J Tissue Viability ; 2021 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-33422386

RESUMO

Perforator flaps have been widely used in clinical Settings, however, unexpected necrosis was still commonly encountered in the distal portions of multiterritory flaps known as Choke zone III. In this study, we introduced a novel hybrid perfusion technique which is different from the established one of arterial supercharging or venous superdrainage to improve multiterritory flap survival with success. In order to ensure the entire flap survival of multiterritory flaps extending to choke zone III, a "hybrid perfusion" mode by anastomosing a distal vein of the flap with a recipient artery was carried out in two cases based on our previous basic study. In addition, a systematic literature review regarding the established microsurgical assistant techniques of arterial supercharging and venous superdrainage techniques were performed. Both flaps survived uneventfully. At a minimal follow-up of six months, both patients were satisfied with the results. This novel hybrid perfusion technique provides a simple new concept in solving partial necrosis of multiterritory flaps. Further practice is guaranteed for better understanding this unconventional attempt.

9.
Nano Lett ; 2021 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-33442978

RESUMO

Morphological coding of nanostructures represents a capability in rapid modulation of structural features and most importantly, the transcription of information into nanoscale. Exploiting the regioselectivity in the template-less electrochemical synthesis of ultrathin Au nanowires, we show that rapid alternation of applied potential would cause corresponding change in the width of the emerging nanowire segments. By understanding the growth kinetics, a strong correlation between the nanowire morphologies and the deposition potential is established and applied in emulating the Morse code.

10.
Oxid Med Cell Longev ; 2021: 6611668, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33505583

RESUMO

The random-pattern flap has a significant application in full mouth restoration (reconstructive surgery) and plastic surgery owing to an easy operation with no axial vascular restriction. However, distal necrosis after flap operation is still considered the most common complication which makes it the Achilles heel in the clinical application of random-pattern flaps. A Chinese medicinal herb named gastrodin is an effective active ingredient of Gastrodia. Herein, the existing study explored the significant potential of gastrodin on flap survival and its underlying mechanism. Our obtained results show that gastrodin will significantly improve flap survival, reduce tissue edema, and increase blood flow. Furthermore, our studies reveal that gastrodin can promote angiogenesis and reduce the apoptotic process as well as oxidative stress. The results of immunohistochemistry and immunoblotting revealed that gastrodin has a role in the elevation of autophagy flux which results in induced autophagy. The use of 3MA (3-methyladenine) for the inhibition of induced autophagy significantly weakened the underlying benefits of gastrodin treatment. Taken together, our obtained results confirmed that gastrodin is an effective drug that can considerably promote the survival rate of flaps (random pattern) via enhancing autophagy. Enhanced autophagy is correlated with the elevation of angiogenesis, reduced level of oxidative stress, and inhibition of cell apoptosis.

11.
Int Immunopharmacol ; 92: 107339, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33516048

RESUMO

BACKGROUND: Inflammation and oxidative stress is closely associated with the development of ischemic brain stroke. Opa-interacting protein 5 antisense RNA 1 (OIP5-AS1), a novel identified long non-coding RNA (lncRNA), has been suggested to play an important role in the development of many types of human cancers. However, the functional involvement of OIP5-AS1 in ischemic stroke is still unknown. METHODS: Quantitative real-time polymerase chain reaction and /or western blot were conducted to determine the expression profiles of OIP5-AS1, C1q/TNF-related protein 3 (CTRP3) and miR-186-5p in the serum of stroke patients, as well as in the ischemic penumbra of rats with middle cerebral artery occlusion/reperfusion (MCAO/R) injury and microglial cells treated with oxygen glucose deprivation/re-oxygenation (OGD/R). Upon selective regulation of OIP5-AS1 and miR-186-5p, the inflammation and oxidative stress responses in microglia/macrophage as well as neurologic functions in MCAO/R rats were detected. Furthermore, the interactions between OIP5-AS1 and miR-186-5p, miR-186-5p and CTRP3 were investigated by RNA immunoprecipitation (RIP) assay, luciferase report assay and bioinformation anaylsis. RESULTS: We observed markedly increased infarct volume, neuronal apoptosis, inflammation and oxidative stress responses in the infarcted lesions of MCAO/R rats, in line with down-regulated levels of OIP5-AS1 and CTRP3 while up-regulated miR-186-5p. Functional studies demonstrated that up-regulation of OIP5-AS1 attenuated infarct volume, neuronal apoptosis, microglia/macrophage inflammation and oxidative stress responses induced by MCAO/R or OGD/R. In terms of mechanism, we revealed that OIP5-AS1-miR-186-5p-CTRP3 axis played a vital role in modulating microglia/macrophage activation and neuronal apoptosis. CONCLUSION: Up-regulating lncRNA OIP5-AS1 protects neuron injury against MCAO/R induced inflammation and oxidative stress in microglia/macrophage through activating CTRP3 via sponging miR-186-5p.

12.
Artigo em Inglês | MEDLINE | ID: mdl-33483307

RESUMO

Within the landscape of globally distributed pathogens, populations differentiate via both adaptive and non-adaptive forces. Individual populations are likely to show unique trends of genetic diversity, host-pathogen interaction, and ecological adaptation. In plant pathogens, allopatric divergence may occur particularly rapidly within simplified agricultural monoculture landscapes. As such, the study of plant pathogen populations in monocultures can highlight the distinct evolutionary mechanisms that lead to local genetic differentiation. Xylella fastidiosa is a plant pathogen known to infect and damage multiple monocultures worldwide. One subspecies, Xylella fastidiosa subsp. fastidiosa was first introduced to the USA ∼150 years ago, where it was found to infect and cause disease in grapevines (Pierce's disease of grapevines, PD). Here, we studied PD-causing subsp. fastidiosa populations, with an emphasis on those found in the USA. Our study shows that following its establishment in the USA, PD-causing strains likely split into populations in the East and West Coast. This diversification has occurred via both changes in gene content (gene gain/loss events) and variations in nucleotide sequence (mutation and recombination). In addition, we reinforce the notion that PD-causing populations within the USA acted as the source for subsequent subsp. fastidiosa outbreaks in Europe and Asia.IMPORTANCE Compared to natural environments, the reduced diversity of monoculture agricultural landscapes can lead bacterial plant pathogens to quickly adapt to local biological and ecological conditions. Because of this, accidental introductions of microbial pathogens into naïve regions represents a significant economic and environmental threat. Xylella fastidiosa is a plant pathogen with an expanding host and geographic range due to multiple intra- and inter-continental introductions. X. fastidiosa subsp. fastidiosa, infects and causes disease in grapevines (Pierce's disease of grapevines; PD). This study focused on PD-causing X. fastidiosa populations, particularly those found in the USA but also invasions into Taiwan and Spain. The analysis shows that PD-causing X. fastidiosa has diversified via multiple co-occurring evolutionary forces acting at an intra- and inter-population level. This analysis enables a better understating of the mechanisms leading to the local adaptation of X. fastidiosa, and how a plant pathogen diverges allopatrically after multiple and sequential introduction events.

13.
Cell Prolif ; 54(2): e12973, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33382502

RESUMO

OBJECTIVES: NLRP3 inflammasome is a critical part of the innate immune system and plays an important role in a variety of inflammatory diseases. However, the effects of NLRP3 inflammasome on periodontitis have not been fully studied. MATERIALS AND METHODS: We used ligature-induced periodontitis models of NLRP3 knockout mice (NLRP3KO ) and their wildtype (WT) littermates to compare their alveolar bone phenotypes. We further used Lysm-Cre/RosanTnG mouse to trace the changes of Lysm-Cre+ osteoclast precursors in ligature-induced periodontitis with or without MCC950 treatment. At last, we explored MCC950 as a potential drug for the treatment of periodontitis in vivo and in vitro. RESULTS: Here, we showed that the number of osteoclast precursors, osteoclast differentiation and alveolar bone loss were reduced in NLRP3KO mice compared with WT littermates, by using ligature-induced periodontitis model. Next, MCC950, a specific inhibitor of the NLRP3 inflammasome, was used to inhibit osteoclast precursors differentiation into osteoclast. Further, we used Lysm-Cre/RosanTnG mice to demonstrate that MCC950 decreases the number of Lysm-Cre+ osteoclast precursors in ligature-induced periodontitis. At last, treatment with MCC950 significantly suppressed alveolar bone loss with reduced IL-1ß activation and osteoclast differentiation in ligature-induced periodontitis. CONCLUSION: Our findings reveal that NLRP3 regulates alveolar bone loss in ligature-induced periodontitis by promoting osteoclastic differentiation.


Assuntos
Perda do Osso Alveolar/patologia , Diferenciação Celular , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Osteoclastos/citologia , Periodontite/patologia , Perda do Osso Alveolar/metabolismo , Perda do Osso Alveolar/prevenção & controle , Animais , Diferenciação Celular/efeitos dos fármacos , Modelos Animais de Doenças , Compostos Heterocíclicos de 4 ou mais Anéis/farmacologia , Compostos Heterocíclicos de 4 ou mais Anéis/uso terapêutico , Inflamassomos/efeitos dos fármacos , Inflamassomos/metabolismo , Interleucina-1beta/metabolismo , Lipopolissacarídeos/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Proteína 3 que Contém Domínio de Pirina da Família NLR/antagonistas & inibidores , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Osteoclastos/metabolismo , Osteogênese/efeitos dos fármacos , Periodontite/tratamento farmacológico , Periodontite/etiologia , Células-Tronco/citologia , Células-Tronco/metabolismo , Sulfonas/farmacologia , Sulfonas/uso terapêutico
14.
Cell Prolif ; 54(2): e12969, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33332682

RESUMO

OBJECTIVES: Various factors could interfere the biological performance of DPSCs during post-thawed process. Yet, little has been known about optimization of the recovery medium for DPSCs. Thus, our study aimed to explore the effects of adding recombinant bFGF on DPSCs after 3-month cryopreservation as well as the underlying mechanisms. MATERIALS AND METHODS: DPSCs were extracted from impacted third molars and purified by MACS. The properties of CD146+ DPSCs (P3) were identified by CCK-8 and flow cytometry. After cryopreservation for 3 months, recovered DPSCs (P4) were immediately supplied with a series of bFGF and analysed cellular proliferation by CCK-8. Then, the optimal dosage of bFGF was determined to further identify apoptosis and TRPC1 channel through Western blot. The succeeding passage (P5) from bFGF pre-treated DPSCs was cultivated in bFGF-free culture medium, cellular proliferation and stemness were verified, and pluripotency was analysed by neurogenic, osteogenic and adipogenic differentiation. RESULTS: It is found that adding 20 ng/mL bFGF in culture medium could significantly promote the proliferation of freshly thawed DPSCs (P4) through suppressing apoptosis, activating ERK pathway and up-regulating TRPC1. Such proliferative superiority could be inherited to the succeeding passage (P5) from bFGF pre-stimulated DPSCs, meanwhile, stemness and pluripotency have not been compromised. CONCLUSIONS: This study illustrated a safe and feasible cell culture technique to rapidly amplify post-thawed DPSCs with robust regenerative potency, which brightening the future of stem cells banking and tissue engineering.


Assuntos
Proliferação de Células/efeitos dos fármacos , Polpa Dentária/metabolismo , Fator 2 de Crescimento de Fibroblastos/farmacologia , Adipogenia/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Antígeno CD146/metabolismo , Diferenciação Celular/efeitos dos fármacos , Criopreservação , Meios de Cultura/química , Polpa Dentária/citologia , Humanos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Proteína Homeobox Nanog/metabolismo , Osteogênese/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Células-Tronco/citologia , Células-Tronco/metabolismo , Canais de Cátion TRPC/antagonistas & inibidores , Canais de Cátion TRPC/metabolismo , Fatores de Tempo , Regulação para Cima/efeitos dos fármacos
15.
Comput Methods Programs Biomed ; : 105864, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33280937

RESUMO

BACKGROUND AND OBJECTIVE: Pathological lung segmentation as a pretreatment step in the diagnosis of lung diseases has been widely explored. Because of the complexity of pathological lung structures and gray blur of the border, accurate lung segmentation in clinical 3D computed tomography images is a challenging task. In view of the current situation, the work proposes a fast and accurate pathological lung segmentation method. The following contributions have been made: First, the edge weights introduce spatial information and clustering information, so that walkers can use more image information during walking. Second, a Gaussian Distribution of seed point set is established to further expand the possibility of selection between fake seed points and real seed points. Finally, the pre-parameter is calculated using original seed points, and the final results are fitted with new seed points. METHODS: This study proposes a segmentation method based on an improved random walker algorithm. The proposed method consists of the following steps: First, a gray value is used as the sample distribution. Gaussian mixture model is used to obtain the clustering probability of an image. Thus, the spatial distance and clustering result are added as new weights, and the new edge weights are used to construct a random walker map. Second, a large number of marked points are automatically selected, and the intermediate results are obtained from the newly constructed map and retained only as pre-parameters. When new seed points are introduced, the probability value of the walker is quickly calculated from the new parameters and pre-parameters, and the final segmentation result can be obtained. RESULTS: The proposed method was tested on 65 sets of CT cases. Quantitative evaluation with different methods confirms the high accuracy on our dataset (98.55%) and LOLA11 dataset (97.41%). Similarly, the average segmentation time (10.5s) is faster than random walker (1,332.5s). CONCLUSIONS: The comparison of the experimental results show that the proposed method can accurately and quickly obtain pathological lung processing results. Therefore, it has potential clinical applications.

16.
Bioresour Technol ; 321: 124496, 2020 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-33302013

RESUMO

In this review investigate the apple orchard waste (AOW) is potential organic resources to produce multi-product and there sustainable interventions with biorefineries approaches to assesses the apple farm industrial bioeconomy. The thermochemical and biological processes like anaerobic digestion, composting and , etc., that generate distinctive products like bio-chemicals, biofuels, biofertilizers, animal feed and biomaterial, etc can be employed for AOW valorization. Integrating these processes can enhanced the yield and resource recovery sustainably. Thus, employing biorefinery approaches with allied different methods can link to the progression of circular bioeconomy. This review article mainly focused on the different biological processes and thermochemical that can be occupied for the production of waste to-energy and multi-bio-product in a series of reaction based on sustainability. Therefore, the biorefinery for AOW move towards identification of the serious of the reaction with each individual thermochemical and biological processes for the conversion of one-dimensional providences to circular bioeconomy.

17.
Front Public Health ; 8: 609974, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33344408

RESUMO

Background: COVID-19 developed into a global pandemic in 2020 and poses challenges regarding the prevention and control capabilities of countries. A large number of inbound travelers from other regions could lead to a renewed outbreak of COVID-19 in the local regions. Globally, as a result of the imbalance in the control of the epidemic, all countries are facing the risk of a renewed COVID-19 outbreak brought about by travelers from epidemic areas. Therefore, studies on a proper management of the inbound travelers are urgent. Methods: We collected a total of 4,733,414 inbound travelers and 174 COVID-19 diagnosed patients in Yunnan province from 21 January 2020 to 20 February 2020. Data on place of origin, travel history, age, and gender, as well as whether they had suspected clinical manifestations for inbound travelers in Yunnan were collected. The impact of inbound travelers on the local epidemic was analyzed with a collinear statistical analysis and the effect of the control measures on the epidemic was evaluated with a sophisticated modeling approach. Results: Of the 174 COVID-19 patients, 60.9% were not from Yunnan, and 76.4% had a history of travel in Hubei. The amount of new daily cases in Yunnan was significant correlated with the number of inbound travelers from Hubei and suspected cases among them. Using Susceptible-Exposed-Infectious-Recovered (SEIR) model analysis, we found that the prevention and control measures dropped the local R0 down to 1.07 in Yunnan province. Conclusions: Our preliminary analysis showed that the proper management of inbound travelers from outbreak areas has a significantly positive effect on the prevention and control of the virus. In the process of resettlement, some effective measures taken by Yunnan province may provide an important reference for preventing the renewed COVID-19 outbreak in other regions.


Assuntos
/transmissão , Surtos de Doenças/prevenção & controle , Surtos de Doenças/estatística & dados numéricos , Transmissão de Doença Infecciosa/prevenção & controle , Pandemias/prevenção & controle , Pandemias/estatística & dados numéricos , Viagem/estatística & dados numéricos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Transmissão de Doença Infecciosa/estatística & dados numéricos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
18.
Artigo em Inglês | MEDLINE | ID: mdl-33191586

RESUMO

By manipulating the nucleation and growth of solid materials, the synthesis of various sophisticated nanostructures has been achieved. Similar methodology, if applied to liquid, could enable the mass-production and control of ultra-small droplets at the scale of nanoparticles (10 -18  L or below). It would be highly desirable since droplets plays a fundamental role in numerous applications. Here we present a general strategy to synthesize and manipulate nanoscale droplets, similar to what has been done to solid nanoparticles in the classis solution-synthesis. It was achieved by a solute-induced phase separation which initiates the nucleation of droplets from a homogeneous solution. These liquid nanoparticles have great potentials to be manipulated like their solid counterparts, borrowing from the vast methodologies of nanoparticle synthesis, such as burst nucleation, seeded growth and co-precipitations. LNPs also serve as a general synthetic platform, to fabricate nanoreactors, drug-loaded carriers, and other hollow nanostructures with a variety of shell materials.

19.
BMC Gastroenterol ; 20(1): 381, 2020 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-33198637

RESUMO

BACKGROUND: Lipid profiles are declined in patients with viral liver cirrhosis and correlated with severity of liver disease. Hepatitis B virus (HBV) is the leading cause of liver cirrhosis in China. Our primary aim was to investigate whether serum lipids and lipoproteins associate with survival in patients with HBV-related cirrhosis and acute gastrointestinal bleeding, and develop a 6-week mortality risk score that incorporates it. METHODS: From January 2008 to December 2015, consecutive cirrhotic patients with acute gastrointestinal bleeding admitted to our hospital were evaluated and randomly divided into the derivation (n = 629) and validation (n = 314) cohorts. A logistic regression model was established to confirm the association between lipoprotein cholesterol and mortality. Accuracy to predict mortality were assessed by area under the receiver operating characteristic curves (AUROCs) and compared using the Hanley and McNeil test. RESULTS: Among study subjects, the 6-week mortality rate was 10.6%. High-density lipoprotein cholesterol (HDL-C) level was found to correlate most strongly with prognostic scores. On ROC analysis, HDL-C showed excellent diagnostic accuracy for 6-week mortality. Logistic regression analysis provided a simple algorithm based on the combined use of 4 variables (total bilirubin (TBIL), HDL-C, International normalized ratio, and hemoglobin), allowing accurate discrimination of 3 distinct prognostic subgroups with 1.7% (low risk), 12.3% (intermediate risk), and 56.9% (high risk) mortality. Its accuracy was significantly better than that of Child-Pugh, model of end-stage liver disease, albumin-bilirubin score, D'Amico model, Augustin model, AIMS65 score and Glasgow-Blatchford score. Baseline HDL-C values ≤ 0.54 mmol/L were associated with markedly lower 6-week survival. Comparable results were found in the validation set. CONCLUSION: HDL-C is a potential indicator for the prognosis of patients with cirrhosis and acute gastrointestinal bleeding. The new algorithm based on HDL-C allowed an accurate predictive assessment of 6-week mortality after bleeding attack.

20.
ACS Nano ; 14(11): 15286-15292, 2020 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-33179490

RESUMO

We study silver nanowires as a model for the mechanical effects of ultrasonication. Their bending is caused by the outward push of shock waves against the inertia and fluid resistance. The structural analyses of a large number of cases reveal the principles of the mechanical effects on the freely suspended colloidal nanostructures. In addition to providing knowledge of the sonication effects, we believe that understanding would help to exploit sonication for nanoscale mechanical manipulation.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA