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1.
J Nanosci Nanotechnol ; 20(2): 769-778, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383072

RESUMO

A super absorbent was synthesized from calcium-aluminum waste residue of aluminum industrial using a facile hydrothermal method. The XRD results revealed that the main phase of hydrothermal product at 120 °C is CaSO4 ·2H2O, with a small amount of Al(OH)3. The as-prepared products were used to investigate the adsorptive applications in Congo red (CR) removal, and the results showed that the products treated at 120 °C had the best adsorption properties. The maximum adsorption capacity reaches about 1860.11 mg/g with a removal rate of 99.75%. Furthermore, the used adsorbent could be regenerated for at least four cycles through a calcination procedure, indicating its potential as an excellent adsorbent for the removal of CR dye from wastewater. The adsorption behavior was analyzed by Langmuir, Freundlich and Sips isotherms, and the adsorption proved to be a multilayer adsorption. This facile method presented here may provide promise synthesis of high-effective and low-cost adsorbents from industrial solid waste and achieve the goal of "using waste to treat waste" in the future.

2.
Int J Syst Evol Microbiol ; 68(10): 3125-3131, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30132753

RESUMO

Phylogenetic analysis was performed on a cellulose-producing strain, designated WE7T, isolated from contaminated coconut milk. The analysis utilized nearly complete 16S rRNA gene sequences, as well as concatenated partial sequences of the housekeeping genes dnaK, groEL and rpoB, and allowed identification of the strain as belonging to the genus Komagataeibacter. DNA-DNA correlation or average nucleotide identity analysis was performed between WE7T and its closest phylogenetic neighbours, and the resulting values were below the species level (<70 % and <95 %), suggesting that the strain represents a novel species in genus Komagataeibacter. Strain WE7T was coupled with Komagataeibacter species more tightly than with Gluconacetobacter species in a 16S rRNA gene sequence phylogenetic tree. Strain WE7T can be differentiated from closely related Komagataeibacter and Gluconacetobacter entanii species by the ability to grow on the carbon sources d-mannitol, sodium d-gluconate and glycerol, the ability to form acid by d-fructose, sucrose, d-mannitol, d-galactose and ethanol, and the ability to grow without acetic acid. The major fatty acid of WE7T is C18 : 1ω9c (52.3 %). The DNA G+C content of WE7T is 63.2 mol%. The name Komagataeibacter cocois sp. nov. is proposed, with the type strain WE7T (=CGMCC 1.15338T=JCM 31140T).


Assuntos
Acetobacteraceae/classificação , Cocos/microbiologia , Alimentos Fermentados/microbiologia , Microbiologia de Alimentos , Filogenia , Acetobacteraceae/genética , Acetobacteraceae/isolamento & purificação , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Genes Bacterianos , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
3.
Sci Rep ; 7(1): 7905, 2017 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-28801648

RESUMO

Primary ciliary dyskinesia (PCD) is clinically characterized by neonatal respiratory distress, chronic sinusitis, bronchiectasis and infertility, and situs inversus in 50% of the patients. PCD is a result of mutations in genes encoding proteins involved in ciliary function, and is primarily inherited in an autosomal recessive fashion. Diagnosis of PCD is often a challenging task due to its high clinical and genetic heterogeneities. In the present study, we attempted to use whole-exome sequencing (WES) combined with runs of homozygosity (ROH) approaches to identify the genetic defects in four Chinese consanguineous families with clinical PCD. We successfully identified three recently acknowledged PCD genes: DYX1C1, CCNO and ARMC4, and one well-characterized PCD gene, DNAI1. Our study provides compelling evidence that WES in combination with ROH analysis is an efficient diagnostic tool for identifying genetic causes of PCD in consanguineous families. Furthermore, our work expands the genetic mutation spectrum in PCD, and provides the additional tools to better serve the counseling of the families with PCD.

4.
Tumour Biol ; 37(5): 6027-34, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26596840

RESUMO

Pentose phosphate pathway (PPP) is a metabolic pathway that generates NADPH and pentose. PPP genes have been reported to be primarily or secondarily upregulated in many cancers. We aimed to study the general alteration of PPP in acute myelogenous leukemia (AML). We performed data mining and analysis of the Cancer Genome Atlas (TCGA) AML dataset for genetic alteration of the PPP gene set. In vitro studies including proliferation, migration, and invasion assays, together with metabolite consumption and oxidation assays, were performed. PPP genes were upregulated in 61 % of patients with AML. The majority of altered cases were expression changes measured by RNA sequencing. Expressions of critical PPP genes such as G6PD, PFKL, PFKP, and PGLS were consistently upregulated in all altered cases. Altered PPP is not associated with survival or disease relapse. PPP inhibition using 6-aminonicotinamide (6AN) increases glucose oxidative metabolism in AML. 6AN decreased the glucose oxidation and increased fatty acid oxidation. Here, we showed that PPP inhibition increased glucose oxidative metabolism in AML. PPP inhibition suppressed growth, migration, and invasion of AML, but not colony formation. PPP plays an important role in AML. Our results could contribute to the development of novel targeted treatment.


Assuntos
Leucemia Mieloide Aguda/metabolismo , Via de Pentose Fosfato , 6-Aminonicotinamida/farmacologia , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Biologia Computacional/métodos , Perfilação da Expressão Gênica , Regulação Leucêmica da Expressão Gênica , Variação Genética , Glucose/metabolismo , Humanos , Leucemia Mieloide Aguda/genética , Leucemia Mieloide Aguda/mortalidade , Oxirredução/efeitos dos fármacos , Prognóstico
5.
Oncol Rep ; 33(4): 2077-85, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25672442

RESUMO

Flavonoids are structurally similar to steroid hormones, particularly estrogens, and therefore have been studied for their potential effects on hormone-dependent cancers. Baicalein is the primary flavonoid derived from the root of Scutellaria baicalensis Georgi. In the present study, we investigated the effects of baicalein on 17ß-estradiol (E2)-induced migration, adhesion and invasion of MCF-7 and SK-BR-3 breast cancer cells. The results demonstrated that baicalein suppressed E2-stimulated wound-healing migration and cell­Matrigel adhesion, and ameliorated E2-promoted invasion across a Matrigel-coated Transwell membrane. Furthermore, baicalein interfered with E2-induced novel G protein-coupled estrogen receptor (GPR30)-related signaling, including a decrease in tyrosine phosphorylation of epidermal growth factor receptor (EGFR) as well as phosphorylation of extracellular signal-regulated kinase (ERK) and serine/threonine kinase Akt, without affecting GPR30 expression. The results also showed that baicalein suppressed the expression of GPR30 target genes, cysteine-rich 61 (CYR61) and connective tissue growth factor (CTGF) induced by E2. Furthermore, baicalein prevented GPR30-related signaling activation and upregulation of CYR61 and CTGF mRNA levels induced by G1, a specific GPR 30 agonist. The results suggest that baicalein inhibits E2-induced migration, adhesion and invasion through interfering with GPR30 signaling pathway activation, which indicates that it may act as a therapeutic candidate for the treatment of GPR30-positive breast cancer metastasis.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/genética , Adesão Celular/efeitos dos fármacos , Estradiol/farmacologia , Flavanonas/farmacologia , Invasividade Neoplásica/genética , Receptores Estrogênicos/genética , Receptores Acoplados a Proteínas-G/genética , Adesão Celular/genética , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Movimento Celular/genética , Fator de Crescimento do Tecido Conjuntivo/genética , Proteína Rica em Cisteína 61/genética , Receptores ErbB/genética , MAP Quinases Reguladas por Sinal Extracelular/genética , Feminino , Humanos , Células MCF-7 , Fosforilação/efeitos dos fármacos , Fosforilação/genética , RNA Mensageiro/genética , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética , Regulação para Cima/efeitos dos fármacos , Regulação para Cima/genética
6.
Mitochondrial DNA ; 26(1): 56-60, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24006865

RESUMO

To detect the somatic mutations and character its spectrum in Chinese lung cancer patients. In this study, we sequenced the whole mitochondrial DNA (mtDNA) genomes for 10 lung cancer patients including the primary cancerous, matched paracancerous normal and distant normal tissues. By analyzing the 30 whole mtDNA genomes, eight somatic mutations were identified from five patients investigated, which were confirmed with the cloning and sequencing of the somatic mutations. Five of the somatic mutations were detected among control region and the rests were found at the coding region. Heterogeneity was the main character of the somatic mutations in Chinese lung cancer patients. Further potential disease-related screening showed that, except the C deletion at position 309 showed AD-weakly associated, most of them were not disease-related. Although the role of aforementioned somatic mutations was unknown, however, considering the relative higher frequency of somatic mutations among the whole mtDNA genomes, it hints that detecting the somatic mutation(s) from the whole mtDNA genomes can serve as a useful tool for the Chinese lung cancer diagnostic to some extent.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Genoma Mitocondrial , Neoplasias Pulmonares/genética , Mutação , China , DNA Mitocondrial , Haplótipos , Humanos , Filogenia , Análise de Sequência de DNA
7.
Cell Biochem Biophys ; 70(1): 391-8, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24676679

RESUMO

Biofilms play a pivotal role in infections related to devices. Biofilm formation in Escherichia coli is mediated by the quorum-sensing E. coli regulator C (QseC), the histidine sensor kinase that can sense epinephrine (EPI)/norepinephrine (NE). In this study, we evaluate the role of the QseC quorum-sensing sensor kinase in epinephrine-enhanced motility and biofilm formation by E. coli. An E. coli MC1000 qseC mutant was constructed. We investigated the role of the QseC in the formation of biofilms on the surface of medical-grade polyvinyl chloride using the E. coli K-12 MC1000 strain as well as a corresponding qseC mutant. Addition of EPI/NE increased biofilm formation by wild-type K-12 MC1000 but not by the isogenic qseC mutant. Scanning confocal laser microscopy corroborated these results by showing that EPI/NE addition significantly increased biofilm's thickness. As expected, the addition of EPI/NE to the qseC mutant, which lacks the ability to sense the hormones, failed to stimulate biofilm formation. Since EPI/NE addition increased bacterial motility, we proposed that their stimulatory effects on biofilm formation occur by enhancing bacterial motility and altering biofilm architecture. We also found that EPI/NE regulate motility and the biofilm phenotype via QseC, as motility was diminished and biofilm formation was significantly decreased in a qseC deletion mutant. These results indicate that EPI/NE induce E. coli biofilm formation on the surface of polyvinyl chloride through QseC. Cross-talk between E. coli (quorum sensing) and host hormones may explain the pathogen-caused opportunistic infections that occur in patients with prosthetic devices used during hormone level fluctuations in the host.


Assuntos
Biofilmes/crescimento & desenvolvimento , Epinefrina/farmacologia , Escherichia coli K12/citologia , Escherichia coli K12/fisiologia , Proteínas de Escherichia coli/metabolismo , Movimento/efeitos dos fármacos , Percepção de Quorum/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Escherichia coli K12/efeitos dos fármacos , Escherichia coli K12/genética , Proteínas de Escherichia coli/genética , Deleção de Genes
8.
Toxicol Appl Pharmacol ; 275(2): 176-81, 2014 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-24440569

RESUMO

Fulvestrant (ICI 182 780, ICI) has been used in treating patients with hormone-sensitive breast cancer, yet initial or acquired resistance to endocrine therapies frequently arises and, in particular, cancer recurs as metastasis. We demonstrate here that both 17-beta-estradiol (E2) and ICI enhance cell adhesion to matrigel in MCF-7 breast cancer cells, with increased autolysis of calpain 1 (large subunit) and proteolysis of focal adhesion kinase (FAK), indicating calpain activation. Additionally, either E2 or ICI induced down-regulation of estrogen receptor α without affecting G protein coupled estrogen receptor 30 (GPR30) expression. Interestingly, GPR30 agonist G1 triggered calpain 1 autolysis but not calpain 2, whereas ER agonist diethylstilbestrol caused no apparent calpain autolysis. Furthermore, the actions of E2 and ICI on calpain and cell adhesion were tremendously suppressed by G15, or knockdown of GPR30. E2 and ICI also induced phosphorylation of extracellular regulated protein kinases 1 and 2 (ERK1/2), and suppression of ERK1/2 phosphorylation by U0126 profoundly impeded calpain activation triggered by estrogenic and antiestrogenic stimulations indicating implication of ERK1/2 in the GPR30-mediated action. Lastly, the E2- or ICI-induced cell adhesion was dramatically impaired by calpain-specific inhibitors, ALLN or calpeptin, suggesting requirement of calpain in the GPR30-associated action. These data show that enhanced cell adhesion by E2 and ICI occurs via a novel GPR30-ERK1/2-calpain pathway. Our results indicate that targeting the GPR30 signaling may be a potential strategy to reduce metastasis and improve the efficacy of antiestrogens in treatment of advanced breast cancer.


Assuntos
Calpaína/metabolismo , Estradiol/análogos & derivados , Antagonistas de Estrogênios/farmacologia , Estrogênios/metabolismo , Receptores Estrogênicos/metabolismo , Receptores Acoplados a Proteínas-G/metabolismo , Neoplasias da Mama/metabolismo , Adesão Celular/efeitos dos fármacos , Colágeno/metabolismo , Regulação para Baixo , Combinação de Medicamentos , Estradiol/farmacologia , Feminino , Fulvestranto , Inativação Gênica , Humanos , Laminina/metabolismo , Sistema de Sinalização das MAP Quinases , Células MCF-7 , Fosforilação , Proteoglicanas/metabolismo , Receptores Estrogênicos/genética , Receptores Acoplados a Proteínas-G/genética , Transdução de Sinais
10.
Med Image Anal ; 16(1): 87-100, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21705264

RESUMO

This study investigates fast detection of the left ventricle (LV) endo- and epicardium boundaries in a cardiac magnetic resonance (MR) sequence following the optimization of two original discrete cost functions, each containing global intensity and geometry constraints based on the Bhattacharyya similarity. The cost functions and the corresponding max-flow optimization built upon an original bound of the Bhattacharyya measure yield competitive results in nearly real-time. Within each frame, the algorithm seeks the LV cavity and myocardium regions consistent with subject-specific model distributions learned from the first frame in the sequence. Based on global rather than pixel-wise information, the proposed formulation relaxes the need of a large training set and optimization with respect to geometric transformations. Different from related active contour methods, it does not require a large number of iterative updates of the segmentation and the corresponding computationally onerous kernel density estimates (KDEs). The algorithm requires very few iterations and KDEs to converge. Furthermore, the proposed bound can be used for several other applications and, therefore, can lead to segmentation algorithms which share the flexibility of active contours and computational advantages of max-flow optimization. Quantitative evaluations over 2280 images acquired from 20 subjects demonstrated that the results correlate well with independent manual segmentations by an expert. Moreover, comparisons with a related recent active contour method showed that the proposed framework brings significant improvements in regard to accuracy and computational efficiency.


Assuntos
Algoritmos , Ventrículos do Coração/patologia , Aumento da Imagem/métodos , Interpretação de Imagem Assistida por Computador/métodos , Imagem Cinética por Ressonância Magnética/métodos , Disfunção Ventricular Esquerda/patologia , Humanos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Processamento de Sinais Assistido por Computador
11.
Int J Syst Evol Microbiol ; 61(Pt 7): 1705-9, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20802064

RESUMO

A gram-positive, non-motile strain, designated CPCC 202695(T), was isolated from a soil sample collected from the Qinghai-Tibet plateau, north-west China. Strain CPCC 202695(T) contained rhamnose, glucose and galactose in the cell wall as diagnostic sugars and 2,4-diaminobutyric acid, alanine, glutamic acid and glycine in the peptidoglycan. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. MK-12 was the predominant menaquinone and anteiso-C(15 : 0) (34.2 %), iso-C(15 : 0) (19.8 %), iso-C(16 : 0) (12.7 %) and anteiso-C(17 : 0) (11.1 %) were the major fatty acids. 16S rRNA gene sequence similarities (94.2-97.1 %) between the isolate and the type strains of recognized species of the genus Agromyces indicated that strain CPCC 202695(T) was a member of the genus Agromyces. DNA-DNA relatedness clearly separated strain CPCC 202695(T) from its closest relatives. The phenotypic and genotypic data demonstrated that strain CPCC 202695(T) represents a novel species of the genus Agromyces, for which the name Agromyces flavus sp. nov. is proposed. The type strain is CPCC 202695(T) ( = KCTC 19578(T)  = CCM 7623(T)).


Assuntos
Actinomycetales/classificação , Filogenia , Microbiologia do Solo , Actinomycetales/genética , Actinomycetales/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Peptidoglicano/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/química
12.
Artigo em Inglês | MEDLINE | ID: mdl-20879249

RESUMO

This study investigates a new parameterization of deformation fields for image registration. Instead of standard displacements, this parameterization describes a deformation field with its transformation Jacobian and curl of end velocity field. It has two important features which make it appealing to image registration: 1) it relaxes the need of an explicit regularization term and the corresponding ad hoc weight in the cost functional; 2) explicit constraints on transformation Jacobian such as topology preserving and incompressibility constraints are straightforward to impose in a unified framework. In addition, this parameterization naturally describes a deformation field in terms of radial and rotational components, making it especially suited for processing cardiac data. We formulate diffeomorphic image registration as a constrained optimization problem which we solve with a step-then-correct strategy. The effectiveness of the algorithm is demonstrated with several examples and a comprehensive evaluation of myocardial delineation over 120 short-axis cardiac cine MRIs acquired from 20 subjects. It shows competitive performance in comparison to two recent segmentation based approaches.


Assuntos
Algoritmos , Coração/anatomia & histologia , Interpretação de Imagem Assistida por Computador/métodos , Imagem Cinética por Ressonância Magnética/métodos , Reconhecimento Automatizado de Padrão/métodos , Técnica de Subtração , Humanos , Aumento da Imagem/métodos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
13.
J Photochem Photobiol B ; 97(1): 18-21, 2009 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-19713121

RESUMO

A sensitive colorimetric sensor (1) based on 4,5-dinitrobenzene-1,2-diamine was designed and synthesized. Binding of anions such as AcO(-), F(-) and H(2)PO(4)(-) results in a notable change in the visible region of spectrum (an approximately 90nm red shift), which can be detected by the 'naked-eye'. Furthermore, the binding ability was evaluated by UV-vis titration experiments as following: AcO(-)>F(-)>H(2)PO(4)(-)>>Cl(-), Br(-), I(-). The nature of the color change of 1 induced by AcO(-) was due to the intramolecular charge transfer (ICT) which was confirmed by X-ray crystal structure and (1)H NMR titration spectra.


Assuntos
Ânions/química , Colorimetria/métodos , Nitrobenzenos/química , Sulfonamidas/química , Cristalografia por Raios X , Diaminas/química , Espectroscopia de Ressonância Magnética , Conformação Molecular , Nitrobenzenos/síntese química , Espectrofotometria Ultravioleta , Sulfonamidas/síntese química
14.
IEEE Trans Med Imaging ; 22(9): 1111-9, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12956266

RESUMO

Mutual information (MI)-based image registration has been found to be quite effective in many medical imaging applications. To determine the MI between two images, the joint histogram of the two images is required. In the literature, linear interpolation and partial volume interpolation (PVI) are often used while estimating the joint histogram for registration purposes. It has been shown that joint histogram estimation through these two interpolation methods may introduce artifacts in the MI registration function that hamper the optimization process and influence the registration accuracy. In this paper, we present a new joint histogram estimation scheme called generalized partial volume estimation (GPVE). It turns out that the PVI method is a special case of the GPVE procedure. We have implemented our algorithm on the clinically obtained brain computed tomography and magnetic resonance image data furnished by Vanderbilt University. Our experimental results show that, by properly choosing the kernel functions, the GPVE algorithm significantly reduces the interpolation-induced artifacts and, in cases that the artifacts clearly affect registration accuracy, the registration accuracy is improved.


Assuntos
Algoritmos , Encéfalo/anatomia & histologia , Ecoencefalografia/métodos , Imagem Tridimensional/métodos , Imagem por Ressonância Magnética/métodos , Processamento de Sinais Assistido por Computador , Técnica de Subtração , Tomografia Computadorizada por Raios X/métodos , Artefatos , Humanos , Estatística como Assunto
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