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1.
BMC Complement Altern Med ; 19(1): 358, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31822288

RESUMO

BACKGROUND: No previous study has investigated the association between oolong tea consumption and esophageal squamous cell carcinoma (ESCC), we aim to elucidate the association between oolong tea consumption and ESCC and its joint effects with a novel composite index. METHODS: In a hospital-based case-control study, 646 cases of ESCC patients and 646 sex and age matched controls were recruited. A composite index was calculated to evaluate the role of demographic characteristics and life exposure factors in ESCC. Unconditional logistic regression was used to calculate the point estimates between oolong tea consumption and risk of ESCC. RESULTS: No statistically significant association was found between oolong tea consumption and ESCC (OR = 1.39, 95% CI: 0.94-2.05). However, drinking hot oolong tea associated with increased risk of ESCC (OR = 1.60, 95% Cl: 1.06-2.41). Furthermore, drinking hot oolong tea increased ESCC risk in the high-risk group (composite index> 0.55) (OR = 3.14, 95% CI: 1.93-5.11), but not in the low-risk group (composite index≤0.55) (OR = 1.16, 95% CI: 0.74-1.83). Drinking warm oolong tea did not influence the risk of ESCC. CONCLUSIONS: No association between oolong tea consumption and risk of ESCC were found, however, drinking hot oolong tea significantly increased the risk of ESCC, especially in high-risk populations.


Assuntos
Dieta/estatística & dados numéricos , Neoplasias Esofágicas/epidemiologia , Carcinoma de Células Escamosas do Esôfago/epidemiologia , Chá , Estudos de Casos e Controles , Feminino , Temperatura Alta , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances
2.
Wei Sheng Yan Jiu ; 48(5): 757-764, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31601316

RESUMO

OBJECTIVE: To elucidate the association between lifestyle and dietary factors and esophageal squamous cell carcinoma( ESCC) in three different sections of the esophagus. METHODS: From January 2010 to December 2016, a hospital-based case-control study was conducted, and a total of 550 patients with ESCC and gender and age( ±3 age) frequency-matched 550 cancer-free control subjects were recruited in this study. Odds ratios( ORs) and their corresponding 95% confidence intervals( CIs) were calculated by using unconditional binary or multinomial logistic regression. Multiple correspondence analysis( MCA) was applied to illustrate the influence of the risk factors on different sections of the esophagus. RESULTS: Tea drinking was associated with lower risk of upper( Ut) and lower thoracic( Lt) ESCC( OR = 0. 40, 95% CI 0. 22-0. 73; OR= 0. 50, 95% CI 0. 31-0. 81; for Ut and Lt, respectively), and lower intake of vegetables increased the risk of Ut and Lt ESCC( OR = 3. 93, 95% CI 1. 61-9. 61; OR =2. 68, 95% CI 1. 30-5. 53; for Ut and Lt, respectively). Intake of hot food, hard food and lower intake of fruits were associated with an elevated risk of the ESCC in all subsites( P<0. 05). The strength of association between drinking and ESCC was lower in middle thoracic( Mt) compared with the Lt ESCC( OR = 0. 58, 95% CI 0. 35-0. 98). Moreover, this reduction of association strength were also found in eating hot food( OR = 0. 45, 95%CI 0. 27-0. 76) and lower intake of vegetables( Ut OR = 0. 44, 95% CI 0. 20-0. 99). However, the association between lower intake of fruits and the Mt ESCC risk was stronger compared with Lt ESCC( OR = 1. 66, 95% CI 1. 08-2. 55). In additional, the association between lower intake of fruits and the Ut ESCC risk was stronger compared with Mt ESCC. Joint category plot of MCA also identified the heterogeneous associations between risk factors and different sections of the esophagus. CONCLUSION: Differences in risk factors of ESCC in different subsites, intake of hot food, hard food, and lower intake of vegetables were common risk factors for three subsites of ESCC.


Assuntos
Dieta/estatística & dados numéricos , Neoplasias Esofágicas/epidemiologia , Carcinoma de Células Escamosas do Esôfago/epidemiologia , Estudos de Casos e Controles , China/epidemiologia , Comportamento Alimentar , Humanos , Estilo de Vida , Fatores de Risco
3.
Postgrad Med J ; 95(1124): 295-299, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31171709

RESUMO

BACKGROUND: Hypertension is the most important modifiable cardiovascular risk factor. Epidemiological studies have shown the benefits of lowering blood pressure (BP), but BP control is a major challenge. Furthermore, there are significant sex differences in antihypertensive drug use and BP control. This study examined sex differences in antihypertensive drug use and BP control, with the aim of reducing the complications of hypertension and improving quality of life. METHODS: The study was performed in our outpatient hypertension clinic, and included 1529 patients without secondary hypertension or comorbidities. The study, investigated BP control rates and patterns of antihypertensive drug use in male and female. All data were collected using structured questionnaires and patient measurements. RESULTS: The study included 713 males and 816 females in this study. Fewer females had hypertension in the younger age group (16.2% vs 11.6%; p>0.05), but this difference disappeared in middle-aged (47.8% vs 49.9 %; p<0.05) and elderly age groups (36.0% vs 38.5%; p<0.05). BP control rates differed between males and females (35.6% in male, 31.9% in female, p<0.01). There was an overall difference in BP control rates between males and females (35.6% in males, 31.9% in females, p<0.01). In this aged 18-44 years, angiotensin converting enzyme inhibitors (ACEIs) showed the best control rate in males, while calcium channel blockers (CCBs) were least effective (61.5% with ACEIs, 28.6% with CCBs; p<0.05). In this aged 45-64 years, diuretics (DUs) showed the best control rate in females, while CCBs were least effective (47.5% with DUs, 28.3% with CCBs; p<0.05). CONCLUSIONS: Sex plays an important role in BP control. In those aged 18-44 years, males using ACEIs showed best control rates. In those aged 45-64 years, females using DUs showed best control rates. Our study provides a basis with the selection of antihypertensive drugs according to sex and age.

4.
Injury ; 50(6): 1237-1241, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31056214

RESUMO

PURPOSE: The aim of the study was to investigate the clinical outcomes of a combined anterior and posterior approach for the surgical treatment of chronic Monteggia fractures in children. MATERIALS AND METHODS: From November 2010 to January 2018, 33 patients (27 boys and 6 girls) with chronic Monteggia fracture who were treated surgically by one surgeon of our department were retrospectively analyzed. In the surgical procedure, open reduction and excision of fibrous scar were performed with the anterior Henry's approach, while ulnar osteotomy was carried out with a posterior approach. In cases of unstable radial head reduction, a trans-capitellar K wire was applied. Repair or reconstruction of the annular ligament (ALR) was not undertaken. RESULTS: The average follow-up of the patients was 33.8 months (range 8-87 months). At the last follow-up, Mayor Score and function of flexion and extension showed significant improvement compared to preoperative condition (p < 0.05). Two patients with palsy of the deep branch of the radial nerve with neurolysis recovered to normal over a 3-month follow-up. Redislocation occurred in two patients while subluxation occurred in one. One patient suffered a mild ischemic contracture but gradually recovered. Other severe complications, nerve injuries, heterotopic ossification, or synostosis, were not noted in the follow-up. CONCLUSION: A combined anterior and posterior approach for surgery resulted in a satisfactory outcome due to the advantages of better exposure, more convenient intraoperative management, and facilitate for radial nerve exploration. Our study provided a new approach for the surgery of chronic Monteggia fractures.

5.
J Cell Biochem ; 120(8): 14116-14126, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30977239

RESUMO

Ectoparasites repress host immune responses while they obtain nutrition from their hosts. Understanding the immunosuppressive mechanisms between ectoparasites and their hosts will provide new strategies to develop potential immunosuppressive drugs against immune disorder diseases. Previously, we have discovered that a small peptide, immunoregulin HA, from the horsefly (Hybomitra atriperoides) may play an immunosuppressive role in rat splenocytes. However, the targeting cells and detailed mechanisms of immunoregulin HA in immunosuppressive reactions are not well defined. Here, we show that immunoregulin HA reduces the secretion of proinflammatory cytokines upon lipopolysaccharide (LPS) stimulation. Interestingly, we discover that the major cytokines repressed by immunoregulin HA are secreted by macrophages, rather than by T cells. Furthermore, immunoregulin HA inhibits macrophage maturation and phagocytosis. Mechanically, the activations of c-JUN N-terminal kinase and extracellular signal-regulated kinase upon LPS stimulation are decreased by immunoregulin HA. Consistently, immunoregulin HA treatment exhibits an anti-inflammatory activity in a mouse model of adjuvant-induced paw inflammation. Taken together, our data reveal that immunoregulin HA conducts the anti-inflammatory activity by blocking macrophage functions.

6.
Oncogene ; 38(23): 4527-4539, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30742065

RESUMO

The altered metabolism and acidic microenvironment plays an important role in promoting tumor malignant characteristics. A small population of cancer stem cells (CSCs) were considered as a therapy target to reserve tumor relapse, resistance, and metastasis. However, the molecular mechanism that regulates CSCs metabolism remains poorly understood. In this study, we demonstrate a fundamental role of stemness gene LIN28B in maintaining CSCs glycolysis metabolism. Using LIN28B-expressing cancer cell lines, we found that the rate of extracellular acidification, glucose uptake, and lactate secretion are all suppressed by LIN28B knockdown in vitro and in vivo. Importantly, metabolic analyses reveal that CSCs have enhanced aerobic glycolysis metabolic characteristics and the glycolytic product lactate further promotes cancer associated stemness properties. LIN28B silencing suppresses MYC expression that further increases miR-34a-5p level. Furthermore, the glycolysis metabolism of human breast cancer cell line MDA-MB-231 is suppressed by either MYC siRNA or miR-34a-5p mimic. Clinically, high MYC and low miR-34a-5p level are correlated with high LIN28B expression and poor prognosis in human breast cancer patients. Notably, blocking LIN28B/MYC/miR-34a-5p signaling pathway by LIN28B-specific inhibitor causes dramatic inhibition of tumor growth and metastasis in immunodeficient orthotopic mouse models of human breast cancer cell MDA-MB-231. Taken together, our findings offer a preclinical investigation of targeting LIN28B to suppress CSCs glycolysis metabolism and tumor progression that may improve the therapeutic benefit for cancer patients.


Assuntos
Regulação Neoplásica da Expressão Gênica , Glucose/metabolismo , Neoplasias/metabolismo , Células-Tronco Neoplásicas/metabolismo , Proteínas de Ligação a RNA/metabolismo , Animais , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Progressão da Doença , Feminino , Glicólise , Humanos , Concentração de Íons de Hidrogênio , Camundongos , Camundongos Endogâmicos NOD , Camundongos SCID , MicroRNAs/metabolismo , Metástase Neoplásica , Recidiva Local de Neoplasia , Transplante de Neoplasias , Prognóstico , RNA Interferente Pequeno/metabolismo , Recidiva , Transdução de Sinais , Microambiente Tumoral
7.
Proc Natl Acad Sci U S A ; 116(7): 2470-2475, 2019 02 12.
Artigo em Inglês | MEDLINE | ID: mdl-30683727

RESUMO

Understanding climate controls on gross primary productivity (GPP) is crucial for accurate projections of the future land carbon cycle. Major uncertainties exist due to the challenge in separating GPP and respiration from observations of the carbon dioxide (CO2) flux. Carbonyl sulfide (COS) has a dominant vegetative sink, and plant COS uptake is used to infer GPP through the leaf relative uptake (LRU) ratio of COS to CO2 fluxes. However, little is known about variations of LRU under changing environmental conditions and in different phenological stages. We present COS and CO2 fluxes and LRU of Scots pine branches measured in a boreal forest in Finland during the spring recovery and summer. We find that the diurnal dynamics of COS uptake is mainly controlled by stomatal conductance, but the leaf internal conductance could significantly limit the COS uptake during the daytime and early in the season. LRU varies with light due to the differential light responses of COS and CO2 uptake, and with vapor pressure deficit (VPD) in the peak growing season, indicating a humidity-induced stomatal control. Our COS-based GPP estimates show that it is essential to incorporate the variability of LRU with environmental variables for accurate estimation of GPP on ecosystem, regional, and global scales.


Assuntos
Umidade , Luz , Fotossíntese , Estômatos de Plantas/fisiologia , Óxidos de Enxofre/metabolismo , Ciclo do Carbono , Ritmo Circadiano , Finlândia , Estômatos de Plantas/metabolismo , Estações do Ano , Taiga
8.
J Pharm Sci ; 107(7): 1879-1885, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29518401

RESUMO

The objective of this study was to investigate the effect of dispersion states of azone in gels on the transdermal permeation of levamisole hydrochloride (LH). LH hydroalcoholic gels containing azone of different dispersion states were prepared by varying the contents of azone and Tween 80, and the in vitro transdermal permeation of LH across excised rat skin was evaluated. Depending on the content of azone, mixed solvents, and solubilizer used, azone presented as dissolved molecules, solubilized in micelles, and fine or coarse emulsion droplets in gels. Dramatically increased transdermal permeation of LH within the azone contents between 0.25% and 0.75% indicated high transdermal enhancement efficiency of the molecular or micellar azone, and extra azone that existed as oil droplets did not fully exert transdermal penetration enhancement of LH. Although solubilizer (Tween 80) can greatly increase the solubility of azone, only small amount of Tween 80 (0.5%) in the gel significantly increased the steady-state flux of LH. Addition of extra amount of Tween 80 (>0.5%) reduced the amount of azone distributed in the skin, and thus decreased the transdermal drug permeation. The results partly elucidated the versatile effects of the dispersion states of azone on the transdermal permeation of hydrophilic drug from semisolid gels.


Assuntos
Antinematódeos/farmacocinética , Antirreumáticos/farmacocinética , Azepinas/química , Hidrogéis/química , Levamisol/farmacocinética , Absorção Cutânea/efeitos dos fármacos , Administração Cutânea , Animais , Antinematódeos/administração & dosagem , Antirreumáticos/administração & dosagem , Azepinas/farmacologia , Hidrogéis/farmacologia , Levamisol/administração & dosagem , Masculino , Micelas , Permeabilidade/efeitos dos fármacos , Ratos Sprague-Dawley , Pele/efeitos dos fármacos , Pele/metabolismo , Solubilidade
9.
Artif Cells Nanomed Biotechnol ; 46(sup1): 1070-1079, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29484961

RESUMO

Highly soluble drugs tend to release from preparations at high speeds, which make them need to be taken at frequent intervals. Additionally, some drugs need to be controlled to release in vivo at certain periods, so as to achieve therapeutic effects. Thus, the objective of this study is to design injectable microparticulate systems with controllable in vivo release profile. Biodegradable PLGA was used as the matrix material to fabricate microspheres using the traditional double emulsification-solvent evaporation method as well as improved techniques, with gel (5% gelatine or 25% F127) or LP powders as the inner phases. Their physicochemical properties were systemically investigated. Microspheres prepared by modified methods had an increase in drug loading (15.50, 16.72, 15.66%, respectively) and encapsulation efficiencies (73.46, 79.42, 74.40%, respectively) when compared with traditional methods (12.01 and 57.06%). The morphology of the particles was characterized by optical microscope (OM) and scanning electron microscopy (SEM), and the amorphous nature of the encapsulated drug was confirmed by differential scanning calorimetry (DSC) and X-ray diffraction (XRD) analysis. To evaluate their release behaviour, the in vitro degradation, in vitro release and in vivo pharmacodynamics were subsequently studied. Traditional microspheres prepared in this study with water as the inner phase had a relatively short release period within 16 d when compared with modified microspheres with 5% gelatine as the inner phase, which resulted in a smooth release profile and appropriate plasma LP concentrations over 21 d. Thus this type of modified microspheres can be better used in drugs requiring sustained release. The other two formulations containing 25% F127 and LP micropowders presented two-stage release profiles, resulting in fluctuant plasma LP concentrations which may be suitable for drugs requiring controlled release. All the results suggested that drug release rates from the microspheres prepared by various methods were mainly controlled by either the porosity inside the microspheres or the degradation of materials, which could, therefore, lead to different release behaviours. This results indicated great potential of the PLGA microsphere formulation as an injectable depot for controllable in vivo release profile via rational core phase design. Core/shell microspheres fabricated by modified double emulsification-solvent evaporation methods, with various inner phases, to obtain high loading drugs system, as well as appropriate release behaviours. Accordingly, control in vivo release profile via rational core phase design.


Assuntos
Microesferas , Ácido Poliglicólico/química , Animais , Preparações de Ação Retardada , Liberação Controlada de Fármacos , Masculino , Ratos , Ratos Sprague-Dawley , Solubilidade , Solventes/química , Distribuição Tecidual , Volatilização
10.
Br J Cancer ; 116(5): 626-633, 2017 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-28081541

RESUMO

BACKGROUND: Circular RNAs (circRNAs) are a class of non-coding RNAs broadly expressed in cells of various species. Their role in cancers, especially in gastric cancer, is poorly understood. METHODS: Circular RNA 0000096 (hsa_circ_0000096) levels in 101 paired gastric cancer tissues and adjacent non-tumorous tissues from patients with gastric cancer were detected by real-time quantitative reverse transcription-polymerase chain reaction. A receiver operating characteristic curve was generated to evaluate the diagnostic value of hsa_circ_0000096. RNA interference was used to manipulate the expression of hsa_circ_0000096. Its biological effects were evaluated by flow cytometry, real-time cell analysis, a wound scratch assay, western blot analysis and xenograft models. RESULTS: Hsa_circ_0000096 was found to be significantly downregulated in gastric cancer tissues and gastric cancer cell lines compared with paired adjacent non-tumorous tissues and normal gastric epithelial cells (P<0.001). Moreover, knockdown of hsa_circ_0000096 significantly inhibited cell proliferation and migration in vitro and in vivo. The results of both immunohistochemical and western blot analyses showed that the protein levels of cyclin D1, cyclin-dependent kinase 6 (CDK6), matrix metalloproteinase-2 and MMP-9 were significantly reduced in vitro and in vivo. A gastric cancer xenograft nude mouse model indicated that Ki67 and VEGF were reduced in a dose-dependent manner following knockdown of hsa_circ_0000096. However, the expression of E-cadherin increased. CONCLUSIONS: Hsa_circ_0000096 may be used as a potential novel biomarker for gastric cancer. It affects gastric cancer cell growth and migration by regulating cyclin D1, CDK6, MMP-2 and MMP-9.


Assuntos
Regulação para Baixo , RNA/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Animais , Proteínas de Ciclo Celular/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos , Transplante de Neoplasias , Valor Preditivo dos Testes , Curva ROC
11.
Proc Natl Acad Sci U S A ; 113(11): 2880-5, 2016 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-26929368

RESUMO

National-scale emissions of carbon tetrachloride (CCl4) are derived based on inverse modeling of atmospheric observations at multiple sites across the United States from the National Oceanic and Atmospheric Administration's flask air sampling network. We estimate an annual average US emission of 4.0 (2.0-6.5) Gg CCl4 y(-1) during 2008-2012, which is almost two orders of magnitude larger than reported to the US Environmental Protection Agency (EPA) Toxics Release Inventory (TRI) (mean of 0.06 Gg y(-1)) but only 8% (3-22%) of global CCl4 emissions during these years. Emissive regions identified by the observations and consistently shown in all inversion results include the Gulf Coast states, the San Francisco Bay Area in California, and the Denver area in Colorado. Both the observation-derived emissions and the US EPA TRI identified Texas and Louisiana as the largest contributors, accounting for one- to two-thirds of the US national total CCl4 emission during 2008-2012. These results are qualitatively consistent with multiple aircraft and ship surveys conducted in earlier years, which suggested significant enhancements in atmospheric mole fractions measured near Houston and surrounding areas. Furthermore, the emission distribution derived for CCl4 throughout the United States is more consistent with the distribution of industrial activities included in the TRI than with the distribution of other potential CCl4 sources such as uncapped landfills or activities related to population density (e.g., use of chlorine-containing bleach).

12.
Ying Yong Sheng Tai Xue Bao ; 26(5): 1531-6, 2015 May.
Artigo em Chinês | MEDLINE | ID: mdl-26571674

RESUMO

Air temperature is the input variable of numerous models in agriculture, hydrology, climate, and ecology. Currently, in study areas where the terrain is complex, methods taking into account correlation between temperature and environment variables and autocorrelation of regression residual (e.g., regression Kriging, RK) are mainly adopted to interpolate the temperature. However, such methods are based on the global ordinary least squares (OLS) regression technique, without taking into account the spatial nonstationary relationship of environment variables. Geographically weighted regression-Kriging (GWRK) is a kind of method that takes into account spatial nonstationarity relationship of environment variables and spatial autocorrelation of regression residuals of environment variables. In this study, according to the results of correlation and stepwise regression analysis, RK1 (covariates only included altitude), GWRK1 (covariates only included altitude), RK2 (covariates included latitude, altitude and closest distance to the seaside) and GWRK2 (co-variates included altitude and closest distance to the seaside) were compared to predict the spatial distribution of mean daily air temperature on Hainan Island on December 18, 2013. The prediction accuracy was assessed using the maximum positive error, maximum negative error, mean absolute error and root mean squared error based on the 80 validation sites. The results showed that GWRK1's four assessment indices were all closest to 0. The fact that RK2 and GWRK2 were worse than RK1 and GWRK1 implied that correlation among covariates reduced model performance.


Assuntos
Clima , Análise Espacial , Temperatura Ambiente , China , Ilhas , Modelos Teóricos , Regressão Espacial
13.
Clin Chim Acta ; 444: 132-6, 2015 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-25689795

RESUMO

BACKGROUND: Circular RNAs (circRNAs), a class of endogenous RNAs, have emerged as an enigmatic class of RNAs. Little is known about their value in the diagnosis of cancers. METHODS: The targeted circRNA of this study was selected using two circRNA databases: CircBase (http://circbase.org/) and circ2Traits (http://gyanxet-beta.com/circdb/). Divergent primers, rather than commonly used convergent primers, for the circRNA were designed. The circRNA levels in 101 paired gastric cancer tissues and adjacent nontumorous tissues from surgical gastric cancer patients and 36 paired plasma samples from preoperative and postoperative gastric cancer patients were analyzed by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The specificity of the amplified products was measured by melting curve analysis and DNA sequencing. To observe the stability of circRNA, three randomly selected samples of gastric cancer tissues were stored at room temperature, 4°C and -20°C, and then, their circRNA levels were analyzed. To verify the reproducibility of qRT-PCR, circRNA levels were detected in a set of specimens (n=15) in two independent experiments with an interval of one day. Then, the correlation of their Ct values was determined. The relationships between circRNA expression levels and clinicopathological factors of patients with gastric cancer were further analyzed by one-way analysis of variance. A receiver operating characteristic (ROC) curve was established to evaluate the diagnostic value. RESULTS: Hsa_circ_002059, a typical circular RNA, was first found to be significantly downregulated in gastric cancer tissues compared with paired adjacent nontumorous tissues (p<0.001). Its levels in plasma collected from postoperative gastric cancer patients were found significantly different from those from preoperative gastric cancer patients. The area under the ROC curve was 0.73. Importantly, we further found that lower expression levels were significantly correlated with distal metastasis (P=0.036), TNM stage (P=0.042), gender (P=0.002) and age (P=0.022). The stability of circRNAs and the reproducibility of the qRT-PCR method for detecting circRNA levels were determined. CONCLUSION: These results suggested that circRNAs are highly stable in mammalian cells and that one specific circRNA, hsa_circ_002059, may be a potential novel and stable biomarker for the diagnosis of gastric carcinoma.


Assuntos
RNA/isolamento & purificação , Neoplasias Gástricas/diagnóstico , Biomarcadores/análise , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , RNA/genética , Reação em Cadeia da Polimerase em Tempo Real , Neoplasias Gástricas/química , Neoplasias Gástricas/genética
14.
PLoS One ; 10(1): e0116774, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25615624

RESUMO

A common problem that is encountered in medical applications is the overall homogeneity of survival distributions when two survival curves cross each other. A survey demonstrated that under this condition, which was an obvious violation of the assumption of proportional hazard rates, the log-rank test was still used in 70% of studies. Several statistical methods have been proposed to solve this problem. However, in many applications, it is difficult to specify the types of survival differences and choose an appropriate method prior to analysis. Thus, we conducted an extensive series of Monte Carlo simulations to investigate the power and type I error rate of these procedures under various patterns of crossing survival curves with different censoring rates and distribution parameters. Our objective was to evaluate the strengths and weaknesses of tests in different situations and for various censoring rates and to recommend an appropriate test that will not fail for a wide range of applications. Simulation studies demonstrated that adaptive Neyman's smooth tests and the two-stage procedure offer higher power and greater stability than other methods when the survival distributions cross at early, middle or late times. Even for proportional hazards, both methods maintain acceptable power compared with the log-rank test. In terms of the type I error rate, Renyi and Cramér-von Mises tests are relatively conservative, whereas the statistics of the Lin-Xu test exhibit apparent inflation as the censoring rate increases. Other tests produce results close to the nominal 0.05 level. In conclusion, adaptive Neyman's smooth tests and the two-stage procedure are found to be the most stable and feasible approaches for a variety of situations and censoring rates. Therefore, they are applicable to a wider spectrum of alternatives compared with other tests.


Assuntos
Análise de Sobrevida , Algoritmos , Humanos , Modelos Estatísticos , Método de Monte Carlo , Modelos de Riscos Proporcionais
15.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 31(2): 336-40, 2014 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-25039138

RESUMO

Low shear stress is a component of the tumor microenvironment in vivo and plays a key role in regulating cancer cell migration and invasion. The integrin, as a mechano-sensors mediating and integrating mechanical and chemical signals, induce the adhesion between cells and extracellular matrix (ECM). The purpose of this study is to investigate the effect of low shear stress (1.4 dyn/cm2)on the migration of HepG2 cells and the expression of integrin. Scratch wound migration assay was performed to examine the effect of low shear stress on the migration of HepG2 cells at 0 h, 1 h, 2 h and 4 h, respectively. F-actin staining was used to detect the expression of F-actin in HepG2 cells treated with low shear stress at 2 h and 4 h. Western blot analysis was carried out to determine the effect of low shear stress on the expression of integrin at different durations. The results showed that the migrated distance of HepG2 cells and the expression of F-actin increased significantly compared with the controls. The integrin alpha subunits showed a different time-dependent expression, suggesting that various subunits of integrin exhibit different effects in low shear stress regulating cancer cells migration.


Assuntos
Movimento Celular , Integrinas/fisiologia , Estresse Mecânico , Actinas/fisiologia , Matriz Extracelular/fisiologia , Células Hep G2 , Humanos
16.
Zhonghua Liu Xing Bing Xue Za Zhi ; 35(5): 600-5, 2014 May.
Artigo em Chinês | MEDLINE | ID: mdl-25059378

RESUMO

To evaluate five methods in the estimation on the rate of case fatality during the epidemics of diseases based on the summarizing data. Case fatality rates, derived from the simulation data, 2003 SARS epidemic data in Hong Kong, Singapore Beijing and the 2013 H7N9 epidemic data in mainland China were analyzed, using these 5 methods. Results from the simulation analysis discovered that the relative errors and the standard deviations of the Chen [7, 8] (method 3), Chen [9] (method 4)were minor with high accuracy. Data from the analysis on 2003 SARS epidemic was noticed that the estimation from method 3, 4 in Hong Kong and Singapore both showing high veracities. Since the case fatality rate reported in Beijing was not a constant value, method 5 showed low accuracy even though it was close to the final case fatality rate. Data from the 2013 H7N9 epidemic showed that the estimations of method 1, 2, 3, 4 were all higher than that in the method 5, suggesting that method 3, 4 could be used to estimate the case fatality rates of epidemics more precisely.


Assuntos
Influenza Humana/mortalidade , Síndrome Respiratória Aguda Grave/mortalidade , China/epidemiologia , Hong Kong/epidemiologia , Humanos , Subtipo H7N9 do Vírus da Influenza A , Análise de Regressão
17.
Tumour Biol ; 35(10): 9591-5, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24961350

RESUMO

Long noncoding RNAs (lncRNAs) have been gradually confirmed to be tumor-associated biological molecules in recent years. However, the clinical significances of most lncRNAs in the screening of gastric cancer are largely unknown. Based on our lncRNA array results, in this study, we explored the potential relationship between HMlincRNA717 expression levels and clinicopathologic factors of gastric cancer. A total of 313 samples were collected from two cancer centers, and then HMlincRNA717 level in human gastric cancer tissues and gastric cell lines was measured by real-time reverse transcription-polymerase chain reaction. Then, HMlincRNA717 levels at multiple stages of gastric tumorigenesis were investigated and the potential association between it levels, and clinicopathological features were analyzed. The expression levels of HMlincRNA717 in five gastric cancer cell lines, AGS, BGC-823, HGC-27, MGC-803, and SGC-7901, were significantly downregulated than those in normal gastric mucosal epithelial cell line GES-1. It was not only downregulated in 62.6 % (67/107) gastric cancer tissues compared with the paired adjacent normal tissues but also in gastric precancerous lesions. More importantly, our results indicated that HMlincRNA717 expression levels were correlated with cancer distal metastasis (P = 0.034), venous invasion (P = 0.029), and nervous invasion (P = 0.024). Our data suggested that lncRNA-HMlincRNA717 may play crucial roles during cancer occurrence and progression and may be a new potential biomarker of early gastric cancer.


Assuntos
Adenocarcinoma/genética , Biomarcadores Tumorais/genética , RNA Longo não Codificante/genética , Neoplasias Gástricas/genética , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Idoso , Linhagem Celular Tumoral , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologia
18.
Ying Yong Sheng Tai Xue Bao ; 25(1): 117-24, 2014 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-24765850

RESUMO

Understanding daily minimum temperature is of great importance for assessing low temperature damages to crops and guiding people to take timely remedial measures to ensure food security. Kriging is a widely used technology for mapping the spatial distribution of the near-surface temperature. However, the smoothing effect, commonly found in the Kriging maps, leads to low values to be overestimated and high values to he underestimated. For daily minimum temperature on Hainan Island which was affected by cold air on December 12, 2011, cross-validation was adopted to evaluate the prediction accuracy of ordinary Kriging (OK) and Kriging with external drift (KED). The spatial distribution maps of daily minimum temperature on Hainan Island on December 12, 2011 produced by OK and sequential Gaussian simulation (SGS) were compared. Results showed that the prediction accuracy of KED (r = 0.86) was not superior to OK (r = 0.86) significantly. SGS could generate multiple equiprobable simulation realizations, and the distribution and variance function of the original data could be reproduced in the realizations. The simulation realizations generated by SGS overcame the smoothing effect of Kriging and could more truly reflect the spatial distribution of minimum temperature on the day on Hainan Island. In the region where daily minimum temperature was low, and the temperature change was small, the conditional variance of the SGS results was less than the ordinary Kriging variance. Spatial uncertainty of a potential chilling damage area could be quantified by multiple simulation realizations generated by SGS. SGS was a valuable tool for assessing agro-meteorological disasters caused by low temperature.


Assuntos
Análise Espacial , Temperatura Ambiente , China , Ilhas , Incerteza
19.
Artigo em Inglês | MEDLINE | ID: mdl-24732215

RESUMO

Corynoxeine(CX), isolated from the extract of Uncaria rhynchophylla, is a useful and prospective compound in the prevention and treatment for vascular diseases. A simple and selective liquid chromatography mass spectrometry (LC-MS) method was developed to determine the concentration of CX in rat plasma. The chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm × 150 mm, 5 µm) column with acetonitrile-0.1% formic acid in water as mobile phase. Selective ion monitoring (SIM) mode was used for quantification using target ions m/z 383 for CX and m/z 237 for the carbamazepine (IS). After the LC-MS method was validated, it was applied to a back-propagation artificial neural network (BP-ANN) pharmacokinetic model study of CX in rats. The results showed that after intravenous administration of CX, it was mainly distributed in blood and eliminated quickly, t1/2 was less than 1h. The predicted concentrations generated by BP-ANN model had a high correlation coefficient (R>0.99) with experimental values. The developed BP-ANN pharmacokinetic model can be used to predict the concentration of CX in rats.


Assuntos
Alcaloides/farmacocinética , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/farmacocinética , Espectrometria de Massas/métodos , Uncaria/química , Administração Oral , Alcaloides/administração & dosagem , Animais , Medicamentos de Ervas Chinesas/administração & dosagem , Alcaloides Indólicos , Masculino , Ratos , Ratos Sprague-Dawley
20.
Ying Yong Sheng Tai Xue Bao ; 20(5): 1105-11, 2009 May.
Artigo em Chinês | MEDLINE | ID: mdl-19803167

RESUMO

A field experiment was conducted in a winter wheat field in Weibei dry highland region of Loess Plateau to study the effects of different mulching and fertilization treatments on soil moisture regime. The treatments were 1) no fertilization, 2) conventional fertilization, 3) recommended fertilization, 4) recommended fertilization + manure, 5) recommended fertilization + plastic mulch on soil ridges, 6) recommended fertilization + plastic mulch on soil ridges and straw mulch in furrows, and 7) recommended fertilization + straw mulch on entire plot. Soil moisture content was determined regularly with a neutron probe. Among the treatments, recommended fertilization plus plastic mulch on soil ridges and straw mulch in furrows in dry season (spring) resulted in the greatest increase of soil water storage and maintained the storage to the critical stage crops needed, followed by recommended fertilization plus plastic mulch on soil ridges. These two treatments could store more precipitation in field, and would benefit the development of rainfed agriculture in dry highland region of Loess Plateau. As for recommended fertilization plus manure, it had the least increase of soil water storage, with a difference of 48.2 mm to the recommended fertilization plus plastic mulch on soil ridges and straw mulch in furrows in dry season.


Assuntos
Agricultura/métodos , Fertilizantes , Solo/análise , Triticum/crescimento & desenvolvimento , Água/análise , Altitude , China
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