Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 18 de 18
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Artigo em Inglês | MEDLINE | ID: mdl-31385379

RESUMO

Genetic interaction has been recognized to be an important cause of the missing heritability. The topologically associating domain (TAD) is a self-interacting genomic region, and the DNA sequences within a TAD physically interact with each other more frequently. Sex differences influence cancer susceptibility at the genetic level. Here, we performed both regular and sex-specific genetic interaction analyses within TAD to identify susceptibility genes for lung cancer in 5204 lung cancer patients and 7389 controls. We found that one SNP pair, rs4262299-rs1654701, was associated with lung cancer in women after multiple testing corrections (combined P = 8.52 × 10-9 ). Single-SNP analyses did not detect significant association signals for these two SNPs. Both identified SNPs are located in the intron region of ANGPT1. We further found that 5% of nonsmall cell lung cancer patients have an alteration in ANGPT1, indicated the potential role of ANGPT1 in the neoplastic progression in lung cancer. The expression of ANGPT1 was significantly down-regulated in patients in lung squamous cell carcinoma and lung adenocarcinoma. We checked the interaction effect on the ANGPT1 expression and lung cancer and found that the minor allele "G" of rs1654701 increased ANGPT1 gene expression and decreased lung cancer risk with the increased dosage of "A" of rs4262299, which consistent with the tumor suppressor function of ANGPT1. Survival analyses found that the high expression of ANGPT1 was individually associated with a higher survival probability in lung cancer patients. In summary, our results suggest that ANGPT1 may be a novel tumor suppressor gene for lung cancer.

2.
Heart Lung ; 48(1): 61-68, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30149956

RESUMO

BACKGROUND: Valvular heart disease is one of the most frequent and challenging heart diseases worldwide. The incidence of complications and cardiothoracic surgical intensive care unit (CSICU) readmission after cardiac valve surgery is high. Because CSICU readmission is costly and adversely impacts the quality life, reducing the risk of CSICU readmission has become one of the main focuses of health care. OBJECTIVE: To explore the risk factors for CSICU readmission and to establish a risk prediction model for CSICU readmission in heart valve surgical patients. METHODS: A total of 1216 patients who had undergone cardiac valvular surgery between January 2016 and August 2017 at the First Affiliated Hospital of Sun Yat-sen University were assigned as the development and validation data sets. Data from 824 patients in the development data set were retrospectively analyzed to identify potential risk factors with univariate analysis. Multivariate logistic regression was used to determine the independent risk factors of CSICU readmission, which served as the basis for our prediction model. The calibration and discrimination of the model were assessed by the Hosmer-Lemeshow (H-L) test and the area under the receiver operating characteristic (ROC) curve, respectively. RESULTS: Six preoperative variables (age ≥ 65, previous chronic lung disease, prior cardiac surgery, left ventricular ejection fraction (LVEF) ≤ 40%, 40% < LVEF ≤ 50%, and New York Heart Association (NYHA) classification III/IV), two intraoperative variables (multiple valve repair/replacement and cardiopulmonary bypass time ≥ 180 min), and five postoperative variables (cardiac arrest, acute respiratory distress syndrome, pneumonia, deep sternal wound infection, and renal failure) were independent risk factors of CSICU readmission. Our risk prediction model, which was established based on the above-mentioned risk factors, had robust discrimination and calibration in both the development and validation data sets. CONCLUSION: The prediction model established in our study is a simple, objective, and accurate scoring system, which can be used to predict the risk of CSICU readmission and assist researchers with designing intervention strategies to prevent CSICU readmission.


Assuntos
Procedimentos Cirúrgicos Cardíacos/métodos , Doenças das Valvas Cardíacas/cirurgia , Valvas Cardíacas/cirurgia , Unidades de Terapia Intensiva/estatística & dados numéricos , Readmissão do Paciente/tendências , Complicações Pós-Operatórias/epidemiologia , Medição de Risco/métodos , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Curva ROC , Estudos Retrospectivos , Fatores de Risco
3.
Brief Bioinform ; 20(1): 26-32, 2019 01 18.
Artigo em Inglês | MEDLINE | ID: mdl-28968709

RESUMO

Genome-wide association studies (GWASs) are an effective strategy to identify susceptibility loci for human complex diseases. However, missing heritability is still a big problem. Most GWASs single-nucleotide polymorphisms (SNPs) are located in noncoding regions, which has been considered to be the unexplored territory of the genome. Recently, data from the Encyclopedia of DNA Elements (ENCODE) and Roadmap Epigenomics projects have shown that many GWASs SNPs in the noncoding regions fall within regulatory elements. In this study, we developed a pipeline named functional disease-associated SNPs prediction (FDSP), to identify novel susceptibility loci for complex diseases based on the interpretation of the functional features for known disease-associated variants with machine learning. We applied our pipeline to predict novel susceptibility SNPs for type 2 diabetes (T2D) and hypertension. The predicted SNPs could explain heritability beyond that explained by GWAS-associated SNPs. Functional annotation by expression quantitative trait loci analyses showed that the target genes of the predicted SNPs were significantly enriched in T2D or hypertension-related pathways in multiple tissues. Our results suggest that combining GWASs and regulatory features data could identify additional functional susceptibility SNPs for complex diseases. We hope FDSP could help to identify novel susceptibility loci for complex diseases and solve the missing heritability problem.


Assuntos
Estudo de Associação Genômica Ampla/estatística & dados numéricos , Polimorfismo de Nucleotídeo Único , Software , Algoritmos , Biologia Computacional , Diabetes Mellitus Tipo 2/genética , Predisposição Genética para Doença , Humanos , Hipertensão/genética , Aprendizado de Máquina , Modelos Genéticos , Modelos Estatísticos , Herança Multifatorial , Locos de Características Quantitativas , Sequências Reguladoras de Ácido Nucleico
4.
J Cancer ; 9(21): 3858-3866, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30410588

RESUMO

Although genome-wide association studies (GWASs) have identified some risk single-nucleotide polymorphisms in East Asian never-smoking females, the unexplained missing heritability is still required to be investigated. Runs of homozygosity (ROHs) are thought to be a type of genetic variation acting on human complex traits and diseases. We detected ROHs in 8,881 East Asian never-smoking women. The summed ROHs were used to fit a logistic regression model which noteworthily revealed a significant association between ROHs and the decreased risk of lung cancer (P < 0.05). We identified 4 common ROHs regions located at 2p22.1, which were significantly associated with decreased risk of lung cancer (P = 2.00 × 10-4 - 1.35 × 10-4). Functional annotation was conducted to investigate the regulatory function of ROHs. The common ROHs were overlapped with potential regulatory elements, such as active epigenome elements and chromatin states in lung-derived cell lines. SOS1 and ARHGEF33 were significantly up-regulated as the putative target genes of the identified ROHs in lung cancer samples according to the analysis of differently expressed genes. Our results suggest that ROHs could act as recessive contributing factors and regulatory elements to influence the risk of lung cancer in never-smoking East Asian females.

5.
Huan Jing Ke Xue ; 39(5): 2202-2210, 2018 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965520

RESUMO

The rG-MnFe2O4 was synthesized by hydrothermal method and characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Raman spectra. The rG-MnFe2O4 was used to activate peroxymonosulfate (PMS) to decolorize azo dyes, e.g., Orange G, and the effect of PMS dosage, rG-MnFe2O4 loadings, initial pH, and the concentration of Cl- were investigated. The results indicated that the degradation rate of OG was 100% within 27 min with 0.3 g ·L-1 of rG-MnFe2O4 and at a 40:1 of PMS:OG molar ratio. The decolorization efficiency of OG increased with increasing PMS concentration and increasing rG-MnFe2O4 dosage. The initial pH had a significant effect on OG degradation, and pH 5.00 was most favorable for its decolorization. In addition, the addition of Cl- accelerated the decolorization of OG, and the decolorization rate increased with increasing concentration of Cl-. The rG-MnFe2O4 also exhibited an excellent reusability, and its activation of PMS was still observed after five rounds of tests. From the analysis of UV-vis spectra and gas chromatography-mass spectrometry (GC/MS), the naphthalene ring and azo band were found to be destroyed, with p-nitrophenol and phthalic acid as the main degradation products. Finally, a TOC analysis indicated that a certain degree of OG mineralization was obtained in the rG-MnFe2O4/PMS system.

6.
Am J Hum Genet ; 102(5): 776-793, 2018 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-29706346

RESUMO

Genome-wide association studies (GWASs) have reproducibly associated variants within intergenic regions of 1p36.12 locus with osteoporosis, but the functional roles underlying these noncoding variants are unknown. Through an integrative functional genomic and epigenomic analyses, we prioritized rs6426749 as a potential causal SNP for osteoporosis at 1p36.12. Dual-luciferase assay and CRISPR/Cas9 experiments demonstrate that rs6426749 acts as a distal allele-specific enhancer regulating expression of a lncRNA (LINC00339) (∼360 kb) via long-range chromatin loop formation and that this loop is mediated by CTCF occupied near rs6426749 and LINC00339 promoter region. Specifically, rs6426749-G allele can bind transcription factor TFAP2A, which efficiently elevates the enhancer activity and increases LINC00339 expression. Downregulation of LINC00339 significantly increases the expression of CDC42 in osteoblast cells, which is a pivotal regulator involved in bone metabolism. Our study provides mechanistic insight into how a noncoding SNP affects osteoporosis by long-range interaction, a finding that could indicate promising therapeutic targets for osteoporosis.


Assuntos
Alelos , Cromossomos Humanos Par 1/genética , Elementos Facilitadores Genéticos , Regulação da Expressão Gênica , Conformação de Ácido Nucleico , Osteoporose/genética , Polimorfismo de Nucleotídeo Único/genética , RNA Longo não Codificante/genética , Grupo com Ancestrais do Continente Asiático/genética , Sequência de Bases , Densidade Óssea/genética , Osso e Ossos/metabolismo , Sistemas CRISPR-Cas/genética , Linhagem Celular , Cromatina/metabolismo , Estudo de Associação Genômica Ampla , Humanos , Modelos Genéticos , Regiões Promotoras Genéticas , Ligação Proteica , Locos de Características Quantitativas/genética , RNA Longo não Codificante/química , Reprodutibilidade dos Testes , Fatores de Risco , Fatores de Transcrição/metabolismo
7.
Hum Genet ; 136(8): 963-974, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28634715

RESUMO

Despite genome-wide association studies (GWASs) have identified many susceptibility genes for osteoporosis, it still leaves a large part of missing heritability to be discovered. Integrating regulatory information and GWASs could offer new insights into the biological link between the susceptibility SNPs and osteoporosis. We generated five machine learning classifiers with osteoporosis-associated variants and regulatory features data. We gained the optimal classifier and predicted genome-wide SNPs to discover susceptibility regulatory variants. We further utilized Genetic Factors for Osteoporosis Consortium (GEFOS) and three in-house GWASs samples to validate the associations for predicted positive SNPs. The random forest classifier performed best among all machine learning methods with the F1 score of 0.8871. Using the optimized model, we predicted 37,584 candidate SNPs for osteoporosis. According to the meta-analysis results, a list of regulatory variants was significantly associated with osteoporosis after multiple testing corrections and contributed to the expression of known osteoporosis-associated protein-coding genes. In summary, combining GWASs and regulatory elements through machine learning could provide additional information for understanding the mechanism of osteoporosis. The regulatory variants we predicted will provide novel targets for etiology research and treatment of osteoporosis.


Assuntos
Osteoporose/genética , Polimorfismo de Nucleotídeo Único , Sequências Reguladoras de Ácido Nucleico , Algoritmos , Linhagem Celular , Galanina/genética , Galanina/metabolismo , Frequência do Gene , Predisposição Genética para Doença , Estudo de Associação Genômica Ampla , Humanos , Modelos Genéticos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Separase/genética , Separase/metabolismo
8.
Artigo em Inglês | MEDLINE | ID: mdl-27688789

RESUMO

Respiratory syncytial virus (RSV) is a common viral pathogen of the lower respiratory tract, which, in the absence of effective management, causes millions of cases of severe illness per year. Many of these infections develop into fatal pneumonia. In a review of English and Chinese medical literature, recent traditional Chinese medical herb- (TCMH-) based progress in the area of prevention and treatment was identified, and the potential anti-RSV compounds, herbs, and formulas were explored. Traditional Chinese medical herbs have a positive effect on inhibiting viral attachment, inhibiting viral internalization, syncytial formation, alleviation of airway inflammation, and stimulation of interferon secretion and immune system; however, the anti-RSV mechanisms of TCMHs are complicated, which should be further investigated.

9.
Sci Rep ; 6: 19868, 2016 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-26879180

RESUMO

Obesity is highly heritable, but the specific genes influencing obesity related traits are largely unknown. Fibroblast growth factor 2 (FGF2) could influence adipocyte differentiation. However, the association of FGF2 polymorphisms and obesity remains unclear. This study aimed to investigate the associations of both the plasma FGF2 levels and SNPs in FGF2 gene with obesity phenotypes in Han Chinese populations. Plasma FGF2 levels were measured and subjected to association analyses in 62 subjects. Eleven SNPs in FGF2 were genotyped and tested for associations in a discovery sample of 1,300 subjects. SNPs significantly associated with obesity were subjected to replication in another independent sample of 1,035 subjects. We found that plasma FGF2 levels were positively correlated with fat mass (P = 0.010). Association analyses in the discovery sample identified three SNPs (rs1449683, rs167428, rs308442) significantly associated with fat mass after multiple testing adjustments (P < 0.0045). Subsequent replication study successfully validated one SNP (rs167428) associated with fat mass (P(combine) = 3.46 × 10(-5)). eQTL analyses revealed that SNPs associated with obesity also affected FGF2 expression. Our findings suggested that high plasma FGF2 level correlated with increased risk of obesity, and FGF2 gene polymorphisms could affect individual variances of obesity in Han Chinese population.


Assuntos
Fator 2 de Crescimento de Fibroblastos/sangue , Fator 2 de Crescimento de Fibroblastos/genética , Obesidade/sangue , Obesidade/genética , Fenótipo , Polimorfismo de Nucleotídeo Único , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático , China , Feminino , Seguimentos , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Desequilíbrio de Ligação , Masculino , Pessoa de Meia-Idade , Obesidade/diagnóstico , Locos de Características Quantitativas , Característica Quantitativa Herdável , Adulto Jovem
10.
Huan Jing Ke Xue ; 37(7): 2591-2600, 2016 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964467

RESUMO

Activated carbon supported cobalt catalysts (Co/AC) were prepared through wet impregnation and high temperature calcination methods. The X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) characterization results showed that Co3O4 was the major form of Co species distributed on AC. The performance of Co/AC was evaluated as catalyst to activate peroxymonosulfate (PMS) to produce sulfate radicals (SO4-·) for Orange G (OG) degradation in solution. The activation mechanism and several influential factors were also investigated. The results demonstrated that SO4-·played a dominant role in OG degradation. And the degradation efficiency of OG increased with increasing Co/AC dosage, higher PMS concentration or elevating reaction temperature. Initial pH had a significant effect on OG degradation, with pH range of 4 to 8 as the optimal pH for degradation. In addition, the strong acidic or alkaline conditions were unfavorable for OG degradation. A dual effect of chloride ions (Cl-) was observed. The high Cl- concentration promoted degradation, while low concentration led to inhibition. The Co/AC also exhibited excellent reusability and its activating performance toward PMS was still observed after 6 rounds of tests. Finally, the degradation process and intermediate products of OG were analyzed with UV-visible spectroscopy and gas chromatography-mass spectrometry (GC/MS).

11.
Huan Jing Ke Xue ; 37(7): 2601-2609, 2016 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964468

RESUMO

Carbon nanotube (CNT) was used as an activator to activate peroxymonosulfate (PMS) to degrade azo dye orange G (OG) in aqueous solution. The results indicated that CNT exhibited a much better performance in activating PMS to decolorize OG than activated carbon (GAC), with 99% decolorization of OG achieved within 45 min. Afterwards, the degradation mechanism of OG in CNT activated PMS system was explored, and SO4-·was found to be dominantly responsible for OG degradation, which mainly took place on the surface of CNT. Effects of various factors, including temperatures, initial concentration of OG, CNT loadings, PMS dosage, and initial pH, on degradation of OG were then investigated, and OG degradation in these cases well conformed to first-order kinetics. From the analysis of UV-vis spectra of OG during the reaction, the peaks at 479 nm and 330 nm were found to be significantly decreased, suggesting that the azo band and naphthaline ring were destructed, respectively. Finally, TOC analysis indicated that a certain degree of OG mineralization was obtained in CNT activated PMS system.

12.
PLoS One ; 10(2): e0117102, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25658585

RESUMO

DDR2 gene, playing an essential role in regulating osteoblast differentiation and chondrocyte maturation, may influence bone mineral density (BMD) and osteoporosis, but the genetic variations actually leading to the association remain to be elucidated. Therefore, the aim of this study was to investigate whether the genetic variants in DDR2 are associated with BMD and fracture risk. This study was performed in three samples from two ethnicities, including 1,300 Chinese Han subjects, 700 Chinese Han subjects (350 with osteoporotic hip fractures and 350 healthy controls) and 2,286 US white subjects. Twenty-eight SNPs in DDR2 were genotyped and tested for associations with hip BMD and fractures. We identified 3 SNPs in DDR2 significantly associated with hip BMD in the Chinese population after multiple testing adjustments, which were rs7521233 (P = 1.06×10-4, ß: -0.018 for allele C), rs7553831 (P = 1.30×10-4, ß: -0.018 for allele T), and rs6697469 (P = 1.59×10-3, ß: -0.015 for allele C), separately. These three SNPs were in high linkage disequilibrium. Haplotype analyses detected two significantly associated haplotypes, including one haplotype in block 2 (P = 9.54×10-4, ß: -0.016) where these three SNPs located. SNP rs6697469 was also associated with hip fractures (P = 0.043, OR: 1.42) in the Chinese population. The effect on fracture risk was consistent with its association with lower BMD. However, in the white population, we didn't observe significant associations with hip BMD. eQTL analyses revealed that SNPs associated with BMD also affected DDR2 mRNA expression levels in Chinese. Our findings, together with the prior biological evidence, suggest that DDR2 could be a new candidate for osteoporosis in Chinese population. Our results also reveal an ethnic difference, which highlights the need for further genetic studies in each ethnic group.


Assuntos
Densidade Óssea , Fraturas por Osteoporose/genética , Polimorfismo de Nucleotídeo Único , Receptores Proteína Tirosina Quinases/genética , Receptores Mitogênicos/genética , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático/genética , China/epidemiologia , Receptores com Domínio Discoidina , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Fraturas por Osteoporose/epidemiologia , Locos de Características Quantitativas , Adulto Jovem
13.
Huan Jing Ke Xue ; 36(11): 4127-34, 2015 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-26910999

RESUMO

Granular activated carbon with silver loaded as activator (Ag/GAC) was prepared using impregnation method. N2 adsorption, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were adopted to characterize the Ag/GAC, showing that silver was successfully loaded on granular activated carbon. The oxidation degradation of acid orange 7 (AO7) by the Ag/GAC activated by persulfate (PS) was investigated at ambient temperature. The influences of factors such as Ag loading, PS or Ag/GAC dosages and initial pH on the degradation of AO7 were evaluated. The results demonstrated that the degradation rate of AO7 could reach more than 95.0% after 180 min when the Ag loading content, PS/AO7 molar ratio, the Ag/GAC dosage were 12.7 mg x g(-1), 120: 1, 1.0 g x L(-1), respectively. The initial pH had significant effect on the AO7 degradation, with pH 5.0 as the optimal pH for the degradation of AO7. The possible degradation pathway was proposed for the AO7 degradation by using UV-visible spectroscopy and gas chromatography-mass spectrometry (GG/MS). The azo bond and naphthalene ring in the AO7 were destroyed during the degradation, with phthalic acid and acetophenone as the main degradation products.


Assuntos
Compostos Azo/química , Benzenossulfonatos/química , Carvão Vegetal/química , Prata/química , Sulfatos/química , Adsorção , Oxirredução , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
14.
Mol Genet Genomics ; 290(2): 485-91, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25300516

RESUMO

Femoral neck (FN) bone mineral density (BMD) is the most important risk phenotype for osteoporosis and has been used as a reference standard for describing osteoporosis. Identification of genetic variations associated with FN BMD may provide potential targets for therapeutic studies. Given the important biological role of FGFR2 gene involved in bone, we tested the associations between FGFR2 polymorphisms and FN BMD in 1,300 Chinese Han subjects. Of the 28 total SNPs, 2 SNPs, namely rs11200014 and rs1078806, were significantly associated with FN BMD under dominant model (P = 0.0014 and 0.0012, respectively) after conservative Bonferroni correction. The two SNPs were in complete linkage disequilibrium. In addition, haplotype-based association tests identified two haplotypes significantly associated with FN BMD, including one haplotype in block 4 where the two SNPs located. However, different from previous studies in white older men, we did not detect any significant association in sex-stratified analyses. In summary, our findings suggest that the FGFR2 gene may play an important role in variation in FN BMD in Chinese Han population, independent of gender effects. Further studies performed in multiple and large samples are needed to elucidate the underlying molecular mechanism and pathophysiology of osteoporosis.


Assuntos
Colo do Fêmur/patologia , Osteoporose/genética , Polimorfismo de Nucleotídeo Único , Receptor Tipo 2 de Fator de Crescimento de Fibroblastos/genética , Adulto , Idoso , Sequência de Bases , Densidade Óssea , Estudos de Casos e Controles , China , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Desequilíbrio de Ligação , Masculino , Pessoa de Meia-Idade , Osteoporose/patologia , Análise de Sequência de DNA
15.
Sci Rep ; 4: 4841, 2014 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-24788080

RESUMO

Environmental pollution by emerging contaminants, e.g. pharmaceuticals, has become a matter of widespread concern in recent years. We investigated the membrane transport of diclofenac and its toxic effects on gene expression and the development of zebrafish embryos. The association of diclofenac with the embryos conformed to the general partition model at low concentration, the partition coefficient being 0.0033 ml per embryo. At high concentration, the interaction fitted the Freundlich model. Most of the diclofenac remained in the extracellular aqueous solution with less than 5% interacting with the embryo, about half of which was adsorbed on the membranes while the rest entered the cytoplasm. Concentrations of diclofenac over 10.13 µM were lethal to all the embryos, while 3.78 µM diclofenac was teratogenic. The development abnormalities at 4 day post treatment (dpt) include shorter body length, smaller eye, pericardial and body edema, lack of liver, intestine and circulation, muscle degeneration, and abnormal pigmentation. The portion of the diclofenac transferred into the embryo altered the expression of certain genes, e.g. down-regulation of Wnt3a and Gata4 and up-regulation of Wnt8a. The alteration of expression of such genes or the regulation of downstream genes could cause defects in the cardiovascular and nervous systems.


Assuntos
Diclofenaco/toxicidade , Regulação da Expressão Gênica/efeitos dos fármacos , Peixe-Zebra/genética , Animais , Anti-Inflamatórios não Esteroides/toxicidade , Ecotoxicologia , Embrião não Mamífero/efeitos dos fármacos , Desenvolvimento Embrionário/efeitos dos fármacos , Desenvolvimento Embrionário/genética , Exposição Ambiental/efeitos adversos , Poluentes Ambientais/toxicidade , Fatores de Tempo
16.
Zhonghua Er Ke Za Zhi ; 51(5): 331-5, 2013 May.
Artigo em Chinês | MEDLINE | ID: mdl-23941837

RESUMO

OBJECTIVE: To improve the understanding of recognizing and diagnosis of neonatal necrotizing enterocolitis (NEC), imaging assessment of neonates with NEC was analyzed retrospectively. METHOD: Data of 211 cases of NEC were retrospectively collected from the Department of Neonatology, Children's Hospital of Chongqing Medical University between Jan.1(st) 2006-Dec.31(st) 2011. RESULT: Analysis of abdominal X-ray of 211 cases showed that there were 40 cases (19.0%) who had no changes on each X-ray, 47 cases (22.3%) had improvement and 23 cases (10.9%) became worse. In the group of no changes, positive rate with good prognosis was 97.5% and with poor prognosis, it was 2.5%. In the group of improvement, positive rate with good prognosis was 97.9%, and the contrary was 2.1%. Positive rate with good prognosis was 56.5%, and the contrary was 43.5% in worse group. Chi-square analysis of the three groups showed χ(2) = 31.742, P < 0.01. Comparison of detection rate of pneumoperitoneum on abdominal X-ray (16.0%, 12/75) and Doppler US (1.3%, 1/75), χ(2) = 10.191, P < 0.05, portal pneumatosis on abdominal X-ray(1.3%, 1/75) versus Doppler US (12.0%,9/75), χ(2) = 6.857, P < 0.05. Surgical timing mostly corresponded to pneumoperitoneum (OR = 19.543) and intestinal obstruction (OR = 19.527) of abdominal X-ray. The logistic regression equation is y = -2.915-1.588x1+2.972x4+2.973x7 + 1.711x9 (χ(2) = 101.705, P < 0.01). CONCLUSION: Abdominal X-ray is the most important method of diagnosis of NEC, the group of deterioration of abdominal X-ray has obvious bad prognosis differ from no change group and better group. Comparison with abdominal X-ray and Doppler US, the former in pneumoperitoneum positive rate was higher than the latter, at the same time, portal pneumatosis on Doppler US is more sensitive to abdominal X-ray, the value of two imaging assessments both supplement each other. Surgical timing mostly corresponds to pneumoperitoneum and intestinal obstruction.


Assuntos
Abdome/diagnóstico por imagem , Enterocolite Necrosante/diagnóstico , Doenças do Recém-Nascido/diagnóstico , Radiografia Abdominal , Abdome/cirurgia , Peso ao Nascer , Enterocolite Necrosante/patologia , Enterocolite Necrosante/cirurgia , Feminino , Humanos , Recém-Nascido , Doenças do Recém-Nascido/patologia , Doenças do Recém-Nascido/cirurgia , Recém-Nascido Prematuro , Perfuração Intestinal/diagnóstico por imagem , Perfuração Intestinal/cirurgia , Modelos Logísticos , Masculino , Pneumoperitônio/diagnóstico , Pneumoperitônio/diagnóstico por imagem , Veia Porta/diagnóstico por imagem , Veia Porta/patologia , Valor Preditivo dos Testes , Prognóstico , Estudos Retrospectivos , Índice de Gravidade de Doença , Ultrassonografia Doppler em Cores
17.
Yi Chuan ; 30(9): 1201-6, 2008 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-18779180

RESUMO

A rice (Oryza sativa L.) plant with white midrib (Oswm2) was selected from the T-DNA inserted mutant pool of rice. The basal midrib of the leaves in the mutant, except for the flag leaves, is white. The blade close to the white midrib is etiolated, and alterations of the agronomic traits, such as plant height, panicle length occur in the mutant. Genetic analysis indicated that this mutant trait was controlled by a recessive nuclear gene. To map the OsWM2 gene, an F2 population was constructed by crossing mutant Oswm2 with 02428. The OsWM2 locus was preliminarily located between the SSR molecular markers RM21478 and RM418 on chromosome 7 with the distances of 8.7 and 15.9 cM, respectively.


Assuntos
Mapeamento Cromossômico , Cromossomos de Plantas/ultraestrutura , Genes de Plantas/fisiologia , Oryza/genética , DNA Bacteriano/isolamento & purificação , DNA Bacteriano/metabolismo , DNA de Plantas/análise , Regulação da Expressão Gênica de Plantas/fisiologia , Mutação , Fotossíntese , Doenças das Plantas/economia , Doenças das Plantas/genética , Infertilidade das Plantas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA