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1.
Foods ; 10(11)2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34828995

RESUMO

Sediment is a key issue in the beverage industry. This study confirmed that reversible and irreversible sediments were formed during low-temperature storage of ginseng extract. The first 30 days of storage are the critical period for sediment formation. As the time of storage extends, the chemical composition changes. The composition interaction model verified that the cross-linking of protein-pectin, protein-oxalic acid and Ca2+-pectin was the main cause of the turbidity of ginseng extract. Based on the characterization of irreversible sediment (IRS), there are typical structures of proteins, polysaccharides and calcium oxalate dihydrate (COD) crystals. Glucose, galacturonic acid, aspartate, glutamic acid, leucine, Ca, K, Al, Mg, Na and Fe are the main monomer components. Effective regulation of these ingredients will greatly help the quality of ginseng beverages.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34558054

RESUMO

The outbreak of COVID-19 has caused increasing public attention to laboratory-acquired infections (LAIs), especially for a mobile Bio-Safety Level 4 Lab (BSL-4) with high potential of exposure. In this paper, the distribution and removal mechanism of bioaerosols in the biosafety laboratory were studied. A simulation model of airflow distribution in the opening and closing state of air-tight door was established and verified. The results showed that the airflow entrainment velocity during the opening of the door was approximately 0.12 m/s. It increased the probability of vortex generation in the laboratory. The deposition rate of particles was doubled when the air-tight door opening is compared with air-tight door closing. Besides, nearly 80% of the particles deposited on the surface of the wall and ceiling, increasing the possibility of LAIs. The findings of this paper could provide new scientific methods for high-level biosafety laboratories to avoid cross-infection. Moreover, future work regarding air-tight door rotation speed regulation and control should be emphasized.

3.
J AOAC Int ; 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34519786

RESUMO

BACKGROUND: At present, PGRs are widely used in agricultural and forestry production in the world, PGRs, like the traditional pesticides have certain toxicity, naively excessive applied them will cause the acute and chronic poisoning of humans and animals, potential harm to human health. OBJECTIVES: In order to assess, prevent and control the residues of plant growth regulators (PGRs) in fruits and vegetables, a set of easy, sensitive, quick, cheap, effective, reliable and safe analytical method that simultaneously detects multiple PGRs residues is urgently needed for the inspection departments of agricultural product's quality safety. METHODS: In this study, grape (representative of fruit) and cabbage (representative of vegetable) were used as the detected objects. The 30 commercial products residues of PGRs in both were detected with the ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS) method based on the optimized chromatographic conditions, mass spectrometry and preparation conditions (extraction solvent and cleanup conditions). Grape and cabbage samples were extracted by acetonitrile containing 5% (v/v) acetic acid, dehydrated by salt package, purified by QuEChERS method, ionized by electrospray (ESI) under positive and negative ion switching mode, detected by multi-reactions monitoring (MRM) and quantification by external standard method of matrix matching standard curve. RESULTS: The results showed that methanol was selected as the strong elution phase. The methanol-0.1% formic acid-5 mmol/L ammonium acetate solution was selected as the best mobile phase. The optimal extraction solvent was acetonitrile containing 5% acetic acid. PSA cleanup could meet the determination requirements of PGRs residues. The developed method for 30 commercial products of PGRs such as betaine showed excellent linearity in 1∼500 µg/kg, 10∼1000 µg/kg, ∼500 µg/kg, ∼2000 µg/kg, and 100∼10000 µg/kg (R ≥ 0.98). At the 0.001 mg/kg (0.01 mg/kg), 0.05 mg/kg, 0.20 mg/kg and 1.00 mg/kg additive concentrations, the average addition standard recovery of 30 commercial products of PGRs were 61%∼132% with the relative standard deviations (RSD) of 1%∼14%, the LOQwere confirmed 1.0-100 µg/kg through the actual addition values of samples. CONCLUSIONS: The results demonstrated that the optimized "QuEChERS-UHPLC-MS/MS method" is a set of simple, rapid, sensitive, accurate, efficient, economical and safe detection method that simultaneously detected the residues of more PGRs in fruits and vegetables through one time sample preparation for their high-throughput rapid quantitative screening and confirmation; it is characterized by wide coverage of detecting PGRs types, simple and convenient preparation and small amount of solvent, and which can provide the technical supports for the supervision of PGRs residues in fruits and vegetables. HIGHLIGHTS: Based on the facts mentioned above, the optimizations of extraction solvent screening, different ratio of various purification packings in QuEChERS method and UPLC-MS conditions were conducted and the indexes of method such as precision, sensitivity and recovery rate were investigated in order to establish an simple, quick, sensitive, cheap, efficient, reliable and safe QuEChERS-UPLC-MS/MS method for simultaneously detecting the 30 kinds of PGRs residues in fruits and vegetables a set of method with simultaneously detecting 30 kinds of PGRs; which shall meet the high throughput determination of multiple PGRs residues in fruits and vegetables and can also provide the technical references for related compounds residues detection of other matrix.

4.
Talanta ; 221: 121613, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33076143

RESUMO

Herein, we developed a simple approach for quantitative metering of nanoliter-scale liquids in parallel based on a capillary array and applied it in high throughput screening protein crystallization conditions. The quantitative metering of liquids was achieved by using capillary force to spontaneously introduce the liquids into short capillaries with fixed length and inner diameter, and the nanoliter-scale droplets were generated by using a pneumatic pump to deliver liquids out from the capillary channels. We adopted measures of sharpening the capillary tips and performing a hydrophobic treatment on the tip surface to significantly reduce the capillary residues during the liquid aspirating and dispensing process, and thus improved the precision to 0.2%-3.5% relative standard deviations (RSD, n = 3) in metering droplets in the range of 280 pL-90 nL. We evaluated the performance of the system in metering liquids of different surface tensions and viscosity. On the basis of this approach, we built a capillary array system with 12 capillaries, by which parallel generation of 12 nL droplets of 12 samples could be achieved in 40 s with a relative standard deviation (RSD) of 1.2%. We applied the system in the screening of lysozyme crystallization conditions of 48 precipitants with 7.5 nL precipitant and 7.5 nL protein solutions in each crystallization droplet reactor, to demonstrate its potentials in large-scale high-throughput screening and analysis with different samples.

6.
Chem Commun (Camb) ; 56(44): 5909-5912, 2020 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-32342091

RESUMO

A three-component reaction between one molecule of phosphorus ylides (P-ylides) and two molecules of isocyanates for the rapid assembly of 2-amino-3-carboxylate-4-quinolones is described. The mechanism may involve the addition of a P-ylide to an isocyanate followed by 1,3-H shift to form a carbamoyl stabilized P-ylide. The intermediate then reacts with another aryl isocyanate via Wittig/ketenimine-ketene rearrangement/6π-electrocyclization/1,3-H shift to finally afford the 4-quinolones.

7.
Angew Chem Int Ed Engl ; 58(31): 10698-10702, 2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31091339

RESUMO

We report a phosphine-catalyzed ring opening of electron-deficient alkylidenecyclopropanes (ACPs) to generate allylic phosphonium zwitterions that resemble the well-studied phosphine-allene adducts but exhibit distinct properties. The potent reactivity of these intermediates has been demonstrated in three types of substrate-controlled phosphine-catalyzed rearrangements of alkylidenecyclopropylketones, which chemoselectively afford tri- and tetrasubstituted furans, and trisubstituted dienones in good yields.

8.
Zhongguo Zhong Yao Za Zhi ; 44(24): 5513-5519, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-32237403

RESUMO

Gansu province,which spans the Yangtze river,is in the upper reaches of the Yellow river and located at the intersection of the Qinghai-Tibet plateau,the Inner Mongolia plateau and the Loess plateau. All of these areas are highly respected by doctors of all ages as they have enriched the resources of traditional Chinese medicines. In the interaction between the heavens and the earth,the interaction between the people and the future has been passed down to the present. As one of the abbreviations of Gansu province, " Long" is not only the symbol and representative of the Gansu region,but also the symbol of the authentic medicinal materials in Gansu. This paper sorts out the evolution of the name " Long" medicines,the development status and development limitations of " Long" medicines. It is believed that although the production areas of authentic medicinal materials in Gansu have changed in different historical periods,the core varieties have been used ever since. Today,with the great development of the Chinese medicine industry,Gansu province pays attention to the limitations of the current regional and technological competitiveness in the province,and gives full play to its own advantages,which can help the " Long" medicines stand out among the medicinal materials. Furthermore,it lays the foundation for the development of the industry and the application of high quality Chinese herbal medicines in clinical practice.


Assuntos
Medicamentos de Ervas Chinesas , Plantas Medicinais , China , Medicina Tradicional Chinesa , Tibet
9.
Zhongguo Zhong Yao Za Zhi ; 43(14): 2837-2844, 2018 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-30111039

RESUMO

Traditional Chinese medicine (TCM) decoction pieces refer to prescription drugs that can be used in clinical or preparation production after processing medicinal herbs. TCM decoction pieces industries are inherited from the culture of TCM and are important because of their independent intellectual property rights. The Chinese Pharmacopoeia (Ch. P) 2010 edition stipulated that "All drugs taken are decoction pieces", which raised the drug status to statutory law for the first time and clearly specified that TCM decoction pieces should be applied to TCM prescription deployment and production of proprietary Chinese medicines. It also pointed out that "The specifications of the decoction pieces used in the preparation should comply with the requirements of the actual process of the corresponding formulation type". For a long time, both the processing methods and the specification grades of the clinically used pieces of Chinese medicine were based on the inheritance and supported by the classical theory and method system centered on TCM processing. However, the theoretical research and specification standards of the decoction pieces used in the production of proprietary Chinese medicines based on modern industry are scarce, and this has led to a series of problems related to the industry, making the processing of decoction pieces becoming a limiting factor in the promotion of the Chinese medicine industry. Aiming at the existing problems of the TCM decoction pieces industry, this article was guided by the standardization system of TCM based on the concept of whole-process quality control, combined with the reference to the Japanese Kampo medicine industry's feeding mode and the reflection on the combination of traditional Chinese medicine processing and modern industry, as well as the study of the core law of the whole-process of TCM production, etc. Industrial decoction pieces and the idea of building a standardized system of TCM industry decoction pieces based on the whole-process quality control were discussed in this paper, which can provide insights for exploring the effective fusion between TCM processing theory and classic heritage and modern manufacturing and can provide the basis for the establishment of a standardized system for industrial decoction pieces based on whole-process quality control of TCM. It can also offer reference for the development of the advantages of geo-authentic crude drug and the establishment of high spots of industry decoction pieces.


Assuntos
Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Prescrições , Controle de Qualidade , Padrões de Referência
10.
Huan Jing Ke Xue ; 39(8): 3502-3510, 2018 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-29998654

RESUMO

To study the pollution characteristics of atmospheric carbon aerosols, aerosol samples were collected via a cascade impactor (Andersen) from March 2014 to February 2015 in Beibei District, Chongqing. Organic carbon (OC) and element carbon (EC) were detected using a DRI 2001A carbon analyzer. The results showed that the annual average concentrations of OC and EC in PM2.1 were (16.3±7.6) and (1.8±0.7), respectively, and (25.0±9.6), and (3.2±1.3) µg·m-3, respectively, in PM9.0. The concentrations of both OC and EC were higher in winter and spring than in summer and autumn for PM2.1, whereas, for PM9.0, the concentration of OC was higher in summer and spring than in winter and autumn and that of EC was higher in winter and spring than in summer and autumn. The particle size distributions of OC and EC for the study year were analyzed, and it was found that those of OC were bimodal, with peaks in the size ranges of 0.43-0.65 µm for fine particles and 4.7-5.8 µm for coarse particles, and those of EC were trimodal, with peaks in the size ranges of 0.43-0.65 µm for fine particles and 4.7-5.8 µm for coarse particles and a concurrent significant peak in the particle size range of 2.1-3.3 µm. In addition, the correlations between OC and EC were analyzed and the SOC in PM2.1 was estimated. It was found that the average concentration of SOC was (6.3±5.9) µg·m-3, which accounted for 33.5%±22.6% of the OC concentration in Beibei District. Furthermore, OC and EC were significantly correlated. Finally, the pollution sources of atmospheric aerosols in Beibei were analyzed, and it was found that the pollution in Beibei mainly came from the exhaust gas of gasoline vehicles, biomass combustion, and coal combustion.

11.
Cell Physiol Biochem ; 45(5): 1975-1985, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29529599

RESUMO

BACKGROUND/AIMS: Long noncoding RNAs (lncRNAs) have recently emerged as novel and potentially promising therapeutic targets in various cancers. However, the expression pattern and biological function of lncRNAs in glioma remain largely elusive. In the present study, we investigated the functional role of an lncRNA, small nucleolar RNA host gene 16 (SNHG16), in glioma. METHODS: The expression levels of SNHG16 and miR-4518 were measured using qRT-PCR. The relationship between the levels of SNHG16 and clinicopathologic features were statically analyzed. The levels of proteins were detected using western blot. Bioinformatics analysis and luciferase reporter assays were applied to the analysis of the relationship between SNHG16, miR-4518 and PRMT5. Cell viability and apoptosis were measured using MTT and apoptosis ELISA assay, respectively. RESULTS: SNHG16 was highly expressed in glioma tissues and cell lines, which was related to poorer clinicopathologic features and shorter survival time. Knockdown of SNHG16 inhibits the viability and induces apoptosis of glioma cells. Further investigation revealed that SNHG16 could up-regulate the expression of miR-4518 targeted gene PRMT5 via acting as an endogenous sponge of miR-4518. Moreover, SNHG16 also affects the expression of Bcl-2 family proteins and the activation of PI3K/Akt signaling pathway. CONCLUSION: Our study revealed a novel SNHG16-miR-4518-PRMT5 pathway regulatory axis in glioma pathogenesis. SNHG16 could be used as a potential therapeutic target in the treatment of glioma.


Assuntos
Neoplasias Encefálicas/patologia , Glioma/patologia , MicroRNAs/metabolismo , Proteína-Arginina N-Metiltransferases/metabolismo , RNA Longo não Codificante/metabolismo , Regiões 3' não Traduzidas , Idoso , Antagomirs/metabolismo , Apoptose , Sequência de Bases , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/mortalidade , Linhagem Celular Tumoral , Proliferação de Células , Intervalo Livre de Doença , Feminino , Glioma/genética , Glioma/mortalidade , Humanos , Estimativa de Kaplan-Meier , Masculino , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Pessoa de Meia-Idade , Fosfatidilinositol 3-Quinases/metabolismo , Proteína-Arginina N-Metiltransferases/antagonistas & inibidores , Proteína-Arginina N-Metiltransferases/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Interferência de RNA , RNA Longo não Codificante/antagonistas & inibidores , RNA Longo não Codificante/genética , RNA Interferente Pequeno/metabolismo , Alinhamento de Sequência , Transdução de Sinais , Regulação para Cima
12.
Nitric Oxide ; 67: 30-38, 2017 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-28433823

RESUMO

A dual-color fluorescence imaging method for simultaneous monitoring of intra- and extracellular nitric oxide (NO) was developed. Assisted by confocal laser scanning microscope, the intra- and extracellular NO can be successfully visualized by using two selected probes, 4,4-difluoro-8-(3,4-diaminophenyl)-3,5-bis(4-methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene (p-MOPB) and disodium 2,6-disulfonate-1,3-dimethyl-5-hexadecyl-8-(3,4-diaminophenyl)-4,4'-difluoro-4-bora-3a,4a-diaza-s-indacene (DSDMHDAB), which display distinct membrane permeability and show different colors of fluorescence after reaction with NO. Results indicated that intra- and extracellular NO could be fluorometrically detected without mutual interference. The applicability of the proposed method was validated by dual-color imaging of NO on both sides of the plasma membrane in RAW 264.7 murine macrophages and human vascular endothelial (ECV-304) cells. This multi-labeling approach using multi-laser excitation and multi-color fluorescence detection holds great promise for simultaneous analysis of NO as well as other gasotransmitters in living cells with subcellular resolution.


Assuntos
Compostos Aza/química , Compostos de Boro/química , Corantes Fluorescentes/química , Óxido Nítrico/análise , Animais , Compostos Azo/farmacologia , Permeabilidade da Membrana Celular , Humanos , Camundongos , Imagem Molecular , Doadores de Óxido Nítrico/farmacologia , Imagem Óptica , Piperazinas/farmacologia , Células RAW 264.7
13.
Spectrochim Acta A Mol Biomol Spectrosc ; 174: 177-182, 2017 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-27907866

RESUMO

As a kind of expensive perfume and valuable herb, Aquilariae Lignum Resinatum (ALR) is often adulterated for economic motivations. In this research, Fourier transform infrared (FT-IR) spectroscopy is employed to establish a simple and quick method for the adulteration screening of ALR. First, the principal chemical constituents of ALR are characterized by FT-IR spectroscopy at room temperature and two-dimensional correlation infrared (2D-IR) spectroscopy with thermal perturbation. Besides the common cellulose and lignin compounds, a certain amount of resin is the characteristic constituent of ALR. Synchronous and asynchronous 2D-IR spectra indicate that the resin (an unstable secondary metabolite) is more sensitive than cellulose and lignin (stable structural constituents) to the thermal perturbation. Using a certified ALR sample as the reference, the infrared spectral correlation threshold is determined by 30 authentic samples and 6 adulterated samples. The spectral correlation coefficient of an authentic ALR sample to the standard reference should be not less than 0.9886 (p=0.01). Three commercial adulterated ALR samples are identified by the correlation threshold. Further interpretation of the infrared spectra of the adulterated samples indicates the common adulterating methods - counterfeiting with other kind of wood, adding ingredient such as sand to increase the weight, and adding the cheap resin such as rosin to increase the content of resin compounds. Results of this research prove that FT-IR spectroscopy can be used as a simple and accurate quality control method of ALR.


Assuntos
Contaminação de Medicamentos , Thymelaeaceae/química , Celulose/química , Lignina/química , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura
14.
Food Chem ; 212: 469-75, 2016 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-27374557

RESUMO

Fourier transform near-infrared (NIR) and mid-infrared (MIR) imaging techniques are essential tools to characterize the chemical morphology of plant. The transmission imaging mode is mostly used to obtain easy-to-interpret spectra with high signal-to-noise ratio. However, the native chemical compositions and physical structures of plant samples may be altered when they are microtomed for the transmission tests. For the direct characterization of thick plant samples, the combination of the reflection NIR imaging and the attenuated total reflection (ATR) MIR imaging is proposed in this research. First, the reflection NIR imaging method can explore the whole sample quickly to find out typical regions in small sizes. Next, each small typical region can be measured by the ATR-MIR imaging method to reveal the molecular structures and spatial distributions of compounds of interest. As an example, the chemical morphology of Areca nut section is characterized directly by the above approach.


Assuntos
Areca/química , Nozes/química , Extratos Vegetais/análise , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Análise de Fourier , Microespectrofotometria/métodos , Estrutura Molecular , Razão Sinal-Ruído , Espectroscopia de Infravermelho com Transformada de Fourier/métodos
15.
Int J Mol Med ; 38(3): 853-60, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27431683

RESUMO

Nuclear receptor subfamily 0 group B member 1 (Nr0b1) is an atypical member of the nuclear receptor family that is predominantly expressed in mouse Sertoli cells (SCs). Mutations of NR0B1 in humans cause adrenal failure and hypogonadotropic hypogonadism. The targeted mutagenesis of Nr0b1 in mice has revealed a primary gonadal defect characterized by the overexpression of aromatase and cellular obstruction of the seminiferous tubules and efferent ductules, leading to germ cell death and infertility. The transgenic expression of Nr0b1 under the control of the Müllerian-inhibiting substance promoter (MIS-Nr0b1), which is selectively expressed in SCs, improves fertility. Testicular androgen receptor (AR) was also expressed in SCs. Many genes are directly regulated by androgen and its AR, which are involved in spermatogenesis and male infertility. As the association between NR0B1 and AR remains unclear in mouse SCs, we decided to further explore the relationship between them. In the present study, we have identified NR0B1 as a novel AR co-repressor in mouse SCs. Using RT­qPCR and immunofluorescence, we determined that NR0B1 was mainly expressed in mouse SCs in an age-dependent manner from 2-8 weeks of age postnatally. The inhibition of the effects of AR on AR target genes by NR0B1, in an androgen­dependent manner, was further demonstrated by western blot analysis and RT-qPCR in TM4 cells, a mouse Sertoli cell line. Finally, in vitro luciferase and co-immunoprecipitation assays validated that NR0B1, as an AR co-repressor, significantly inhibited the transcriptional activation of its target genes. These results suggest that novel inhibitory mechanisms underlie the effects of NR0B1 in modulating androgen-dependent gene transcription in mouse SCs.


Assuntos
Proteínas Correpressoras/genética , Receptor Nuclear Órfão DAX-1/genética , Receptores Androgênicos/genética , Células de Sertoli/metabolismo , Fatores Etários , Androgênios/metabolismo , Androgênios/farmacologia , Animais , Western Blotting , Linhagem Celular , Proteínas Correpressoras/metabolismo , Receptor Nuclear Órfão DAX-1/metabolismo , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Células HEK293 , Humanos , Masculino , Camundongos , Microscopia Confocal , Ligação Proteica , Interferência de RNA , Receptores Androgênicos/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Testículo/crescimento & desenvolvimento , Testículo/metabolismo
16.
Mol Med Rep ; 14(2): 1290-6, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27278724

RESUMO

Distinguishing the testes-specific genes in different species may disclose key genes associated with testes-specific functions and provide sufficient information for the study and treatment of male infertility. A testes­specific gene, coiled-coil domain containing 38 (Ccdc38), was identified by screening UniGene libraries. Systematic bioinformatics analysis demonstrated that the CCDC38 protein was conserved in various mammalian species. It was determined that CCDC38 was exclusively expressed in testes and its expression increased from 2­8 weeks of age. Additional immunohistochemical analysis indicated that CCDC38 was mainly expressed in spermatogonia and spermatocytes. It is of note that, immunofluorescence and co-immunoprecipitation assays demonstrated that CCDC38 interacted with ubiquitinated histone H2A in mouse testes. Therefore, these results suggest that Ccdc38 is a testes-specific gene, which may be important for mouse spermatogenesis.


Assuntos
Expressão Gênica , Testículo/metabolismo , Animais , Feminino , Perfilação da Expressão Gênica , Histonas/metabolismo , Imuno-Histoquímica , Infertilidade Masculina/genética , Masculino , Camundongos , Especificidade de Órgãos/genética , Filogenia , Ligação Proteica , Transporte Proteico , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Espermatócitos/metabolismo , Espermatogênese/genética , Espermatogônias/metabolismo , Ubiquitinas/metabolismo
17.
Spectrochim Acta A Mol Biomol Spectrosc ; 165: 176-182, 2016 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-27156099

RESUMO

Herbal powder preparation is a kind of widely-used herbal product in the form of powder mixture of herbal ingredients. Identification of herbal ingredients is the first and foremost step in assuring the quality, safety and efficacy of herbal powder preparations. In this research, Fourier transform infrared (FT-IR) microspectroscopic identification method is proposed for the direct and simultaneous recognition of multiple organic and inorganic ingredients in herbal powder preparations. First, the reference spectrum of characteristic particles of each herbal ingredient is assigned according to FT-IR results and other available information. Next, a statistical correlation threshold is determined as the lower limit of correlation coefficients between the reference spectrum and a larger number of calibration characteristic particles. After validation, the reference spectrum and correlation threshold can be used to identify herbal ingredient in mixture preparations. A herbal ingredient is supposed to be present if correlation coefficients between the reference spectrum and some sample particles are above the threshold. Using this method, all kinds of herbal materials in powder preparation Kouqiang Kuiyang San are identified successfully. This research shows the potential of FT-IR microspectroscopic identification method for the accurate and quick identification of ingredients in herbal powder preparations.


Assuntos
Medicamentos de Ervas Chinesas/química , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Algoritmos , Pós , Controle de Qualidade
18.
Tumour Biol ; 37(9): 11775-11787, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27029387

RESUMO

Cell plasma membrane proteins, playing a crucial role in cell malignant transformation and development, were the main targets of tumor detection and therapy. In this study, CyDye/biotin double-labeling proteomic approach was adopted to profile the membrane proteome of gastric cancer cell line BGC-823 and paired immortalized gastric epithelial cell GES-1. Real-time PCR, Western blotting, and immunohistochemical staining were used to validate the differential expression of a novel identified cell surface marker R-cadherin in gastric cancer cells and tissues. Clinicopathological study and survival analysis were performed to estimate its roles in tumor progression and outcome prediction. Real-time PCR and Western blotting showed that the expression level of R-cadherin in gastric cancer were significantly lower than non-cancerous epithelial cell and tissues. Clinicopathological study indicated that R-cadherin was dominantly expressed on cell surface of normal gastric epithelium, and its expression deletion in gastric cancer tissues was associated with tumor site, differentiation, lymph node metastasis, and pTNM (chi-square test, P < 0.05). Those patients with R-cadherin positive expression displayed better overall survivals than negative expression group (log-rank test, P = 0.000). Cox multivariate survival analysis revealed lacking the expression of R-cadherin was a main independent predictor for poor clinical outcome in gastric cancer (RR = 5.680, 95 % CI 2.250-14.341, P < 0.01). We have established a fundamental membrane proteome database for gastric cancer and identified R-cadherin as a tumor differentiation and progression-related cell surface marker of gastric cancer. Lacking the expression of R-cadherin indicates poor prognosis in patients with gastric cancer.


Assuntos
Biomarcadores Tumorais/metabolismo , Caderinas/metabolismo , Proteínas de Membrana/metabolismo , Proteoma/metabolismo , Proteômica/métodos , Neoplasias Gástricas/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/genética , Western Blotting , Caderinas/genética , Diferenciação Celular , Linhagem Celular , Linhagem Celular Tumoral , Progressão da Doença , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Imuno-Histoquímica , Estimativa de Kaplan-Meier , Masculino , Proteínas de Membrana/genética , Proteínas de Membrana/isolamento & purificação , Pessoa de Meia-Idade , Análise Multivariada , Prognóstico , Proteoma/genética , Proteoma/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia
19.
Zhonghua Nan Ke Xue ; 22(1): 12-6, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-26931019

RESUMO

OBJECTIVE: To investigate the expression characteristics of the gene of coiled-coil domain-containing protein 70 (Ccdc70) in the mouse testis and its potential role in spermatogenesis. METHODS: Using expression profile microarray, we screened the mouse testis-specific gene Ccdc70, studied its expression characteristics in the mouse testis by RT-PCR, real-time PCR, Western blot and immunohistochemistry, followed by bioinformatic analysis of the Ccdc70 protein. RESULTS: The Ccdc70 gene was expressed highly in the testis but lowly in the epididymis of the mice. The Ccdc70 protein was expressed mainly in the spermatocytes and round spermatids of the testis and in the epithelial cells of the epididymis. Bioinformatic analysis showed a structural domain in the Ccdc70 protein, which was highly conserved in mammalian evolution. CONCLUSION: The Ccdc70 gene is highly expressed in the mouse testis and mainly in the spermatocytes, round spermatids, and epididymal epithelial cells, which indicates that it is involved in the regulation of spermatogenesis and epididymal sperm maturation.


Assuntos
Proteínas/genética , Espermatogênese/genética , Testículo/metabolismo , Animais , Biologia Computacional , Regulação da Expressão Gênica no Desenvolvimento , Masculino , Camundongos
20.
Zhongguo Zhong Yao Za Zhi ; 41(8): 1485-1492, 2016 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-28884544

RESUMO

Armand clematis stem (Clematidis Armandii Caulis, Chuanmutong) is a widely used Chinese herb to disinhibit urine and relieve stranguria. It is difficult to be identified owing to its various macroscopic feature and unknown characteristic compounds. Thus, total of 24 Chuanmutong samples and 7 related herbs including four manshurian aristolochia stem (Aristolochiae Manshuriensis Caulis, Guanmutong) and three akebia stem (Akebiae Caulis, Mutong) samples were collected and analyzed in the range of 4 000 - 400 cm⁻¹ by Fourier Transform Infrared (FTIR) and two-dimensional infrared correlation spectroscopy (2D-FTIR) techniques. The FTIR spectra of 24 Chuanmutong samples are consistent in the spectrum profiles, position and intensity of characteristic peaks. 20 of the 24 Chuanmutong samples were randomly selected as calibration samples to calculate and simulate mean spectrum. This mean spectrum is named as FTIR fingerprint of Chuanmutong with characteristic peaks at 3 412, 2 932, 1 739, 1 639, 1 509, 1 456, 1 426, 1 376, 1 332, 1 261, 1 159, 1 035, 897 ,609 cm⁻¹. Meanwhile, the limited level (Mean-3σ=0.992 6) to identify true or false Chuanmutong by correlation coefficient of FTIR spectra was calculated based on the 20 Chuanmutong calibration samples. Then, the rest 4 Chuanmutong, 4 Guanmutong and 3 Mutong samples were used as validation samples to evaluate the identification efficacy. The result shows that the FTIR spectra of 4 Chuanmutong validation samples were similar to the fingerprint. Their correlation coefficients of FTIR spectra were over the limited level and accepted as Chuanmutong. However, the spectra of Guanmutong and Mutong were significantly different from Chuanmutong fingerprint. The correlation coefficients of Guanmutong (0.902 1-0.940 4, n=4) and Mutong (0.954 9-0.978 9, n=3) FTIR spectra were less than the limited level and rejected from Chuanmutong. Furthermore, the number, position and intensity of auto-peaks on the 2D-FTIR were drastically different among the three herbs. It is concluded that the developed FTIR fingerprinting can be rapidly and accurately identify Chuanmutong and differentiate from related herbs.


Assuntos
Clematis/química , Medicamentos de Ervas Chinesas/química , Caules de Planta/química , Aristolochia/química , Asteraceae/química , Espectrofotometria Infravermelho , Espectroscopia de Infravermelho com Transformada de Fourier
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