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1.
Inorg Chem ; 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32167759

RESUMO

Metal-organic frameworks (MOFs) based on 9,10-diphenylanthracene-derived ligands had been reported to exhibit upconverted fluorescence through triplet-triplet annihilation. We found that zirconium MOFs based on 9,10-diphenylanthracene can also give upconverted fluorescence via two-photon absorption without adding a triplet photosensitizer when a femtosecond pulsed laser is used as the excitation source. By tuning the synthetic condition, we obtained nanoscale MOFs of UiO structure in both octahedral and hexagonal nanoplate shapes, as well as a hexagonal nanoplate of MOFs of hcp-UiO structure and two-dimensional metal-organic layers. All of them, as well as a homogeneous solution of the 9,10-diphenylanthracene ligand, exhibit upconverted fluorescence upon excitation using a laser pulse of 60 fs with a pulse energy of ∼1.1 × 106 nJ/cm2 (unfocused). Moreover, we observed different emission spectra by two-photon excitation compared to those by one-photon excitation, which indicates access to a unique initial excited state via two-photon excitation. This phenomenon is not observed for a homogeneous solution of the ligand. These nanoscale MOFs may find application in two-photon fluorescence imaging.

2.
Colloids Surf B Biointerfaces ; 189: 110833, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32058254

RESUMO

The implimentation of newer technologies in drug delivery system have always been the focus of pharmaceutical scientists with advancement of technologies. In this investigation, a novel controlled-release drug-resin combination device (DRC) was designed using dental resin to control the release of dextromethorphan hydrobromide (DH). The influence of different factors on in-vitro drug release were investigated. A Box-Behnken design was used to select the optimized DRC formulation. The optimized DH loaded DRC (DH-DRC) was prepared using 59.88% of PEG400, 16 mg of dental resin and 6.64 mg of sodium chloride (NaCl). The DH releases at 2 h, 4 h and 8 h of the optimized formulation were significantly close to the predicted responses. The pharmacokinetic study in rabbits showed DH-DRC had prolonged tmax and apparently reduced Cmax compared with commercial tablets and the AUC0-24h of DH-DRC was slightly higher than commercial tablets. This study confirmed the novel DRC could control the release of drug. It concluded that DRC would be a promising and alternative approach for the development of controlled release dosage form.

3.
J Mol Diagn ; 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32068069

RESUMO

Differences in the mutation spectrum across ethnicities suggest the importance of identifying genes in addition to common high penetrant genes to estimate the associated breast cancer risk in Chinese. A total of 1338 high-risk breast cancer patients who tested negative for germline BRCA1, BRCA2, TP53, and PTEN mutations between 2007 and 2017 were selected from the Hong Kong Hereditary Breast Cancer Family Registry. Patient samples were subjected to next-generation DNA sequencing using a multigene panel (Color Genomics). All detected pathogenic variants were validated by bidirectional DNA sequencing. The sequencing data were coanalyzed by a bioinformatics pipeline developed in-house. Sixty-one pathogenic variants (4.6%) were identified in this cohort in 11 cancer predisposition genes. Most carriers (77.1%) had early onset of breast cancer (age <45 years), 32.8% had family members with breast cancer, and 11.5% had triple-negative breast cancer. The most common mutated genes were PALB2 (1.4%), RAD51D (0.8%), and ATM (0.8%). A total of 612 variants of unknown significance were identified in 494 patients, and 87.4% of the variants of unknown significance were missense mutations. Pathogenic variants in cancer predisposition genes beyond BRCA1, BRCA2, TP53, and PTEN were detected in an additional 4.6% of patients using the multigene panel. PALB2 (1.4%) and RAD51D (0.8%) were the most commonly mutated genes in patients who tested mutation negative by a four-gene panel.

4.
Environ Res ; 183: 109156, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-32000003

RESUMO

The selective degradation of recalcitrant antibiotics into byproducts with low toxicity and high biodegradability has been increasingly popular using peroxymonosulfate (PMS) based advanced oxidation processes (AOPs). In this paper, two Fe-based heterogeneous catalysts, bentonite supported Fe-Ni composite (BNF) and biochar-supported Fe composite (Fe/C), were tailored and comprehensively characterized for distinctive physicochemical properties, crystalline structures, and interfacial behaviors. Two widely used antibiotics, sulfapyridine (SPY) and oxytetracycline (OTCs) at their common concentrations in pharmaceutical wastewaters (250 and 10 mg L-1) were tested for degradation in three PMS-based oxidation processes, i.e., PMS, PMS-BNF, and PMS-Fe/C, respectively. Results demonstrated that a large amount of PMS (10 and 1 mM) could effectively remove SPY (0.385 min-1, 100% removal) and OTC (2.737 min-1, 100% removal) via1O2 derived from PMS self-decomposition and non-radical pathway, respectively. Additional Fe-based catalysts (0.5 g L-1 Fe/C and BNF) significantly reduced the PMS consumption (1 and 0.25 mM) and accelerated the reaction rate (1.08 and 5.05 min-1) of SPY and OTC removal. Moreover, the supplementary catalysts shifted the degradation route. The biochar matrix in Fe/C composite contributed to predominant interaction with PMS forming 1O2, which preferably attacked SPY via hydroxylation. In contrast, the redox-active Fe-Ni pairs induced SO4- formation, which could selectively degrade OTC through decarboxylation. Thus, these results are conducive to tailoring advanced yet low-cost heterogeneous catalysts for eco-friendly treatment of antibiotics-rich industrial wastewaters.

5.
BMC Genomics ; 21(1): 68, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31964339

RESUMO

BACKGROUND: Global climate change is predicted to force the bathymetric migrations of shallow-water marine invertebrates. Hydrostatic pressure is proposed to be one of the major environmental factors limiting the vertical distribution of extant marine invertebrates. However, the high-pressure acclimation mechanisms are not yet fully understood. RESULTS: In this study, the shallow-water sea cucumber Apostichopus japonicus was incubated at 15 and 25 MPa at 15 °C for 24 h, and subjected to comparative transcriptome analysis. Nine samples were sequenced and assembled into 553,507 unigenes with a N50 length of 1204 bp. Three groups of differentially expressed genes (DEGs) were identified according to their gene expression patterns, including 38 linearly related DEGs whose expression patterns were linearly correlated with hydrostatic pressure, 244 pressure-sensitive DEGs which were up-regulated at both 15 and 25 MPa, and 257 high-pressure-induced DEGs which were up-regulated at 25 MPa but not up-regulated at 15 MPa. CONCLUSIONS: Our results indicated that the genes and biological processes involving high-pressure acclimation are similar to those related to deep-sea adaptation. In addition to representative biological processes involving deep-sea adaptation (such as antioxidation, immune response, genetic information processing, and DNA repair), two biological processes, namely, ubiquitination and endocytosis, which can collaborate with each other and regulate the elimination of misfolded proteins, also responded to high-pressure exposure in our study. The up-regulation of these two processes suggested that high hydrostatic pressure would lead to the increase of misfolded protein synthesis, and this may result in the death of shallow-water sea cucumber under high-pressure exposure.

6.
Indoor Air ; 30(2): 213-234, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31709614

RESUMO

Low-cost airborne particle sensors are gaining attention for monitoring human exposure to indoor particulate matter. This study aimed to establish the concentrations at which these commercially available sensors can be expected to report accurate concentrations. We exposed five types of commercial integrated devices and three types of "bare" low-cost particle sensors to a range of concentrations generated by three different sources. We propose definitions of upper and lower bounds of functional range based on the relationship between a given sensor's output and that of a reference instrument during a laboratory experiment. Experiments show that the lower bound can range from approximately 3 to 15 µg/m3 . At greater concentrations, sensor output deviates from linearity at approximately 300-3000 µg/m3 . We also conducted a simulation campaign to analyze the effect of this limitation on functional range on the accuracy of exposure readings given by these devices. We estimate that the upper bound results in minimal inaccuracy in exposure quantification, and the lower bound can result in as much as a 50% error in approximately 10% of US homes.

7.
Dig Liver Dis ; 52(2): 233, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31843254
8.
J Hazard Mater ; 383: 121240, 2020 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-31563767

RESUMO

High-efficiency and cost-effective catalysts are critical to completely mineralization of organic contaminants for in-situ groundwater remediation via advanced oxidation processes (AOPs). The engineered biochar is a promising method for waste biomass utilization and sustainable remediation. This study engineers maize stalk (S)- and maize cob (C)-derived biochars (i.e., SB300, SB600, CB300, and CB600, respectively) with oxygen-containing functional groups as a carbon-based support for nanoscale zero-valent iron (nZVI). Morphological and physiochemical characterization showed that nZVI could be impregnated within the framework of the synthesized Fe-CB600 composite, which exhibited the largest surface area, pore volume, iron loading capacity, and Fe0 proportion. Superior degradation efficiency (100% removal in 20 min) of trichloroethylene (TCE, 0.1 mM) and fast pseudo-first-order kinetics (kobs =22.0 h-1) were achieved via peroxymonosulfate (PMS, 5 mM) activation by the Fe-CB600 (1 g L-1) under groundwater condition (bicarbonate buffer solution at pH = 8.2). Superoxide radical and singlet oxygen mediated by Fe0 and oxygen-containing group (i.e., CO) were demonstrated as the major reactive oxygen species (ROSs) responsible for TCE dechlorination. The effectiveness and mechanism of the Fe/C composites for rectifying organic-contaminated groundwater were depicted in this study.

9.
Cell Death Dis ; 10(12): 919, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31801939

RESUMO

TMEM45A is a transmembrane protein involved in tumor progression and cancer resistance to chemotherapeutic agents in hypoxic condition. It is correlated to a low breast cancer patient overall survival. However, little is known about this protein, in particular the mechanisms by which TMEM45A modulates cancer cell chemosensitivity. In this work, the messenger RNA expression of TMEM45A was assessed in head and neck squamous cell carcinoma (HNSCC) and renal cell carcinoma (RCC) biopsies. TMEM45A was upregulated in patients diagnosed for head and neck or renal cancer. Then, the implication of this protein in cisplatin sensitivity was explored in SQD9 and RCC4 + pVHL cells. TMEM45A inactivation decreased cell proliferation and modulated cell responses to cisplatin. Indeed, TMEM45A inactivation increased the sensitivity of SQD9 cells to cisplatin, whereas it rendered RCC4 + pVHL cells resistant to this anticancer agent. Through RNA-sequencing analysis, we identified several deregulated pathways that indicated that the impact on cisplatin sensitivity may be associated to the inhibition of DNA damage repair and to UPR pathway activation. This study demonstrated, for the first time, an anti or a pro-apoptotic role of this protein depending on the cancer type and highlighted the role of TMEM45A in modulating patient responses to treatment.

10.
Mol Immunol ; 116: 151-159, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31675522

RESUMO

The interleukin-17 (IL-17) family plays a critical role in host defense, allergic reactions, and even tumorigenesis on different mucous membranes. IL-17 family has been cloned in human and mouse, as well IL-17A, IL-17 F in swine. So far, current knowledge on the cloning and biological functions of porcine IL-17B (poIL-17B) and porcine IL-17E (poIL-17E) is limited. In this study, poIL-17B and poIL-17E, mainly expressed in intestine, were cloned and characterized. Expression of poIL-17B and poIL-17E was upregulated after pathogenic microorganism infection. Moreover, the significant enhanced expression of antibacterial peptides PR-39 and pBD-1 was observed when poIL-17B and poIL-17E were over-expressed in the small intestinal epithelial cell line IPEC-J2. This demonstrated that poIL-17B and poIL-17E might have anti-infective capability. Pathogens infection data showed that pathogens could up-regulate poIL-17B/E expression levels. After stimulating the cells with the pathogen, continued with probiotics, the expression of poIL-17B/E was down-regulated. Meanwhile, the induced expression of poIL-17E was greater than that of poIL-17B. Invasion data indicated that poIL-17B and poIL-17E both could inhibit effectively pathogenic microorganism, while inhibitory capability of poIL-17B was stronger than that of poIL-17E. Therefore, poIL-17B and poIL-17E both could be important members against intestinal infection in the porcine IL-17 family. This study provided a theoretical basis for the prevention of intestinal diseases in pigs and thus achieved healthy farming.


Assuntos
Infecções Bacterianas/imunologia , Interleucina-17/imunologia , Intestinos/imunologia , Intestinos/microbiologia , Animais , Linhagem Celular , Regulação para Baixo/imunologia , Células Epiteliais/imunologia , Células Epiteliais/microbiologia , Humanos , Camundongos , Probióticos/administração & dosagem , Suínos , Regulação para Cima/imunologia
11.
Artigo em Inglês | MEDLINE | ID: mdl-31714237

RESUMO

In this article, a neural network (NN)-based robust adaptive fault-tolerant control (FTC) algorithm is proposed for a class of multi-input multi-output (MIMO) strict-feedback nonlinear systems with input quantization and actuation faults as well as asymmetric yet time-varying output constraints. By introducing a key nonlinear decomposition for quantized input, the developed control scheme does not require the detailed information of quantization parameters. By imposing a reasonable condition on the gain matrix under actuation faults, together with the inherent approximation capability of NN, the difficulty of FTC design caused by anomaly actuation can be handled gracefully, and the normally used yet rigorous assumption on control gain matrix in most existing results is significantly relaxed. Furthermore, a brand new barrier function is constructed to handle the asymmetric yet time-varying output constraints such that the analysis and design are extremely simplified compared with the traditional barrier Lyapunov function (BLF)-based methods. NNs are used to approximate the unknown nonlinear continuous functions. The stability of the closed-loop system is analyzed by using the Lyapunov method and is verified through a simulation example.

12.
World Neurosurg ; 132: e76-e88, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31518750

RESUMO

OBJECTIVE: The tumor microenvironment greatly influences tumor formation, invasion, and progression. The ESTIMATE (Estimation of STromal and Immune cells in MAlignant Tumor tissues) algorithm quantifies stromal and immune components in a tumor, reflecting the tumor microenvironment. This study aimed to explore key prognostic genes in a grade II/III glioma microenvironment. METHODS: We obtained stromal/immune scores for the Cancer Genome Atlas (TCGA) grade II/III glioma cohort from the online ESTIMATE portal. The associations of stromal/immune scores with clinicopathologic characteristics and overall survival of patients with grade II/III glioma were assessed by the Mann-Whitney U test and the Kaplan-Meier method, respectively. Functional enrichment analysis and protein-protein interaction network assessments were employed to analyze differentially expressed genes (DEGs). The top 7 genes with 5 or more edges in the protein-protein interaction network were selected. For validation, CGGA grade II/III glioma data were analyzed. RESULTS: The results showed that elevated stromal/immune/ESTIMATE score was significantly associated with poor survival of patients with TCGA grade II/III glioma. Functional enrichment analysis showed that DEGs were associated with immune cell regulation, extracellular matrix, cytokine activation, and receptor binding. The selected DEGs (interleukin-10, beta-2 microglobulin, C-C motif chemokine ligand 5, cluster of differentiation 74, human leukocyte antigen-DRA, lymphocyte cytosolic protein 2, and myxovirus resistance protein 1) showed prognostic values in patients with grade II/III glioma of the TCGA and CGGA database. CONCLUSIONS: Stromal/immune/ESTIMATE scores have prognostic values in patients with grade II/III glioma. The selected DEGs, including interleukin-10, beta-2 microglobulin, C-C motif chemokine ligand 5, cluster of differentiation 74, human leukocyte antigen-DRA, lymphocyte cytosolic protein 2, and myxovirus resistance protein 1, associated with tumor immunity and microenvironment, have prognostic values in grade II/III glioma. Further investigation of these genes could provide novel insights into the tumor microenvironment of glioma.


Assuntos
Neoplasias Encefálicas/genética , Neoplasias Encefálicas/patologia , Glioma/genética , Glioma/patologia , Microambiente Tumoral , Adulto , Idoso , Idoso de 80 Anos ou mais , Algoritmos , Biomarcadores Tumorais , Neoplasias Encefálicas/imunologia , Estudos de Coortes , Mineração de Dados , Bases de Dados Factuais , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Glioma/imunologia , Humanos , Imunidade Celular , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Prognóstico , Mapas de Interação de Proteínas , Análise de Sobrevida
13.
J Neurooncol ; 145(2): 201-210, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31506754

RESUMO

PURPOSE: Overexpression of CD44 has been detected in many types of tumor tissues. Moreover, CD44 is recognized as a cancer stem cell marker for many cancers. However, the prognostic value of CD44 for glioma patients has not yet been clarified. The authors tried to explore the impact of CD44 expression on grade II/III glioma patients. METHODS: To assess the RNA expression levels of CD44 in glioma tissues and normal brain tissues, meta-analyses were conducted in the online Oncomine database. The mRNA expression levels of CD44, CD44s, and CD44v2-v10 in 112 grade II/III glioma patients in Hokkaido University Hospital (HUH) were detected by qPCR. The RNA-seq data and clinical data of grade II/III glioma patients were obtained from The Cancer Genome Atlas (TCGA) and the Chinese Glioma Genome Atlas (CGGA) databases. RESULTS: Based on the Oncomine database, CD44 has significantly high expression in glioma tissues as compared with normal tissues. We explored the clinical relevance of CD44 mRNA expression based on the HUH cohorts, the TCGA cohorts, and the CGGA cohorts. In survival analysis, high mRNA expression of CD44 was correlated with poor overall survival and poor progression-free survival in grade II/III glioma patients. Multivariate Cox regression analyses confirmed CD44 as an independent prognostic factor for grade II/III glioma patients. CONCLUSIONS: The present study suggests that overexpression of CD44 is associated with a poor prognosis for grade II/III glioma patients. Moreover, our findings suggest that CD44 could serve as a prognostic biomarker in grade II/III glioma patients.

15.
Environ Pollut ; 254(Pt B): 113123, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31487672

RESUMO

Biochar has potential to control the bioavailability and migration of potentially toxic heavy metals in soil by adsorption. Natural ageing in the environment may change the physicochemical properties and adsorption function of biochar over the long-term. The present study compared the effects of different simulated ageing treatments on Cd adsorption of high and low temperature biochar from straw of corn (Zea mays). Fresh and aged biochars were systematically characterized by elemental analysis, FTIR, XPS, Zeta, SEM-EDS, XRD and the composition of their mineral ash. The adsorption of Cd to fresh and aged biochars was then assessed under the influence of pH. Drawing the results together the effects of ageing on the extent and mode of Cd adsorption could be elucidated. The results showed that the adsorption capacity of fresh biochar produced at 650 °C was higher than of biochar made at 350 °C, and that mineral co-precipitation plays a dominant role in Cd sorption. Leaching removed organic and inorganic ash components from biochars, markedly diminishing the capacity of the high temperature biochar to adsorb Cd. The adsorption performance of the low temperature biochar was dependent on surface complexation. The adsorption capacity of low-temperature biochar was markedly enhanced by oxygen-containing functional groups formed through acidification and oxidation. The long-term benefits of biochar in the management of polluted soil require a rethink, considering the contrasting ageing behavior of different temperature biochar and their response to different ageing environments.


Assuntos
Cádmio/química , Carvão Vegetal/química , Poluentes do Solo/química , Adsorção , Cádmio/análise , Temperatura Alta , Concentração de Íons de Hidrogênio , Minerais , Oxirredução , Solo/química , Tempo , Zea mays
16.
Surg Oncol ; 31: 46-53, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31536927

RESUMO

Acetylcholine (ACh) was first identified as a classic neuromodulator and transmit signals through two subgroups of receptors, namely muscarinic receptors (mAChRs) and nicotinic receptors (nAChRs). Apart from its well-established physiological role in central nervous system (CNS) and peripheral nervous system (PNS), autonomic nervous system and neuromuscular junction, the widely distributed expression of AChRs in different human organs suggests roles in other biological processes in addition to synaptic transmission. Accumulating evidence revealed that cancer cell processes such as proliferation, apoptosis, angiogenesis and even epithelial-mesenchymal transition (EMT) are mediated by overexpression of AChRs in different kinds of tumors. In breast cancer, α7-nAChR and α9-nAChR were reported to be oncogenic. On the other hand, research on the role of mAChRs in breast cancer tumorgenesis is limited and confined to M3 receptor only. Since AChRs distributed in both CNS and PNS even non-neuronal tissues, there is an urgent need for the development of subtype-specific AChR antagonist which inhibits cancer cell progression with minimal intervention on the normal acetylcholine-regulated system within human body.

17.
ChemSusChem ; 12(20): 4543-4569, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31386795

RESUMO

Catalytic hydrosilylation of carbon dioxide has emerged as a promising approach for carbon dioxide utilization. It allows the reductive transformation of carbon dioxide into value-added products at the levels of formate, formaldehyde, methanol, and methane. Tremendous progress has been made in the area of carbon dioxide hydrosilylation since the first reports in 1981. This focus review describes recent advances in the design and catalytic performance of leading catalyst systems, including transition-metal, main-group, and transition-metal/main-group and main-group/main-group tandem catalysts. Emphasis is placed on discussions of key mechanistic features of these systems and efforts towards the development of more selective, efficient, and sustainable carbon dioxide hydrosilylation processes.

18.
BMC Urol ; 19(1): 78, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31438919

RESUMO

BACKGROUND: Lower urinary tract symptoms (LUTS) is the most common complication of diabetes. However, the underlying pathogenesis of cultured negative LUTS (cn-LUTS) in diabetic patients has not been well understood. Numerous evidence indicates that urinary dysbiosis is related to urologic disorders. We aim to study alterations of the urinary microbiota of cn-LUTS in type 2 diabetes (T2D) patients. METHODS: Female T2D patients and controls were recruited and requested to finish the American Urological Association Symptom Index. Mid-stream urine was collected for culturing and extracting DNA. Microbial diversity and composition were analyzed by targeting to 16S rDNA. Linear discriminant analysis effect size (LEfSe) was carried out to identify significantly different bacteria. RESULTS: 32 female T2D patients and 26 controls were enrolled. No significant differences in alpha diversity were observed between patients and controls. However, statistically decreased richness (ACE index and Chao 1 index, 85.52(13.75, 204.84) vs. 129.82(63.89, 280.30) and 83.86(11.00, 210.77) vs. 125.19(62.00, 251.77), P = 0.005; Observed Species, 76(10, 175) vs. 98(54, 234), P = 0.011) and decreased species diversity (Shannon index, 1.37(0.04, 3.48) vs. 2.09(0.98, 3.43), P = 0.033; Simpson index, 0.46 (0.06, 0.99) vs. 0.23(0.07, 0.64), P = 0.029) were shown in moderate-to-severe LUTS group and high Hemoglobin A1c group, respectively. A significant difference of beta diversity was found between T2D patients and controls and T2D patients with different severity of cn-LUTS as well as the different level of Hemoglobin A1c. LEfSe revealed that 10 genera (e.g., Escherichia-Shigella and Klebsiella) were increased and 7 genera were decreasing in T2D patients, 3 genera (e.g., Escherichia-Shigella and Campylobacter) were increased and 16 genera (e.g., Prevotella) were reduced in moderate-to-severe LUTS group, 2 genera (Escherichia-Shigella and Lactobacillus) were over-represented and 10 genera (e.g., Prevotella) were under-represented in high Hemoglobin A1c group. Finally, Hemoglobin A1c was found positively correlated with the total score of the American Urological Association Symptom Index (r = 0.509, P = 0.003). CONCLUSIONS: Urinary dysbiosis may be related to cn-LUTS in female T2D patients. A better understanding of urinary microbiota in the development and progression of cn-LUTS in female T2D patients was necessary. The severity of cn-LUTS was correlated to hyperglycemia and chronic hyperglycemia might induce or promote cn-LUTS by influencing urinary microbiota.


Assuntos
Complicações do Diabetes/microbiologia , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/microbiologia , Sintomas do Trato Urinário Inferior/etiologia , Sintomas do Trato Urinário Inferior/microbiologia , Microbiota , Idoso , Estudos Transversais , Feminino , Humanos , Pessoa de Meia-Idade
19.
IEEE Trans Cybern ; 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31425063

RESUMO

Ensemble learning performs better than a single classifier in most tasks due to the diversity among multiple classifiers. However, the enhancement of the diversity is at the expense of reducing the accuracies of individual classifiers in general and, thus, how to balance the diversity and accuracies is crucial for improving the ensemble performance. In this paper, we propose a new ensemble method which exploits the correlation between individual classifiers and their corresponding weights by constructing a joint optimization model to achieve the tradeoff between the diversity and the accuracy. Specifically, the proposed framework can be modeled as a shallow network and efficiently trained by the end-to-end manner. In the proposed ensemble method, not only can a high total classification performance be achieved by the weighted classifiers but also the individual classifier can be updated based on the error of the optimized weighted classifiers ensemble. Furthermore, the sparsity constraint is imposed on the weight to enforce that partial individual classifiers are selected for final classification. Finally, the experimental results on the UCI datasets demonstrate that the proposed method effectively improves the performance of classification compared with relevant existing ensemble methods.

20.
Mol Cell Biochem ; 462(1-2): 85-96, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31446614

RESUMO

Heat shock proteins (HSPs) may be induced by hypoxia and alleviate blood-brain barrier (BBB) damage. The neuroprotective effect of propofol has been reported. We aimed to identify whether propofol induced HSPs expression and protected BBB integrity. Mouse astrocytes and microglia cells were cultured and exposed to hypoxia and propofol. The expression of HSP27, HSP32, HSP70, and HSP90, and the translocation of heat shock factor 1 (HSF1) and Nuclear factor-E2-related factor 2 (Nrf2) were investigated. Mouse brain microvascular endothelial cells, astrocytes, and microglial cells were co-cultured to establish in vitro BBB model, and the effects of hypoxia and propofol as well as HSPs knockdown/overexpression on BBB integrity were measured. Hypoxia (5% O2, 5% CO2, 90% humidity) treatment for 6 h and 12 h induced HSP27, HSP32, and HSP70 expression. Propofol (25 µΜ) increased HSP27 and HSP32 expression, starting with exposure to hypoxia for 3 h. Propofol induced HSF1 translocation from cytoplasmic to nuclear compartment, and blockade of HSF1 inhibited HSP27 expression in mouse astrocytes when they were exposed to hypoxia for 3 h. Propofol induced Nrf2 translocation, and blockade of Nrf2 inhibited HSP32 expression in mouse microglial cells when they were exposed to hypoxia for 3 h. Propofol protected hypoxia-impaired BBB integrity, and the effects were abolished by blockade of HSF1 and Nrf2. Overexpression of HSP27 and HSP32 alleviated hypoxia-impaired BBB integrity, and blockade of HSP27 and HSP32 expression ameliorated propofol-mediated protection against BBB impairment. Propofol may protect hypoxia-mediated BBB impairment. The mechanisms may involve HSF1-mediated HSP27 expression and Nrf2-mediated HSP32 expression.


Assuntos
Barreira Hematoencefálica/metabolismo , Proteínas de Choque Térmico/metabolismo , Propofol/farmacologia , Animais , Barreira Hematoencefálica/efeitos dos fármacos , Hipóxia Celular/efeitos dos fármacos , Camundongos , Fator 2 Relacionado a NF-E2/metabolismo , Permeabilidade , Substâncias Protetoras/farmacologia
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