Metal ion and hydrogen bonding synergistically mediated carboxylated lignin/cellulose nanofibrils composite film.

In order to alleviate the resource and environmental problems caused by plastic film materials, the development of biodegradable cellulose-based films is crucial. Inspired by the strengthening mechanism of cellulose-lignin network from wood, carboxylated lignin (CL) was isolated using maleic acid (MA) pretreatment catalyzed by metal chlorides. Compared with pure MA, the presence of metal ions yielded CL with high carboxyl content (0.34 mmol/g), small size and good dispersibility. CL was then composited with CNF to prepare various CL/cellulose nanofibrils (CNF) composite films. When the addition of ferric chloride was 0.3 mmol/g maleic acid, the corresponding composite films exhibited highest tensile strength (180.0 MPa), Young's modulus (13.0 GPa) and excellent ultraviolet blocking rate (97.0 %). Meanwhile, the interaction forces measured by atomic force microscope showed that the binding between CNF and various CLs (276-406 nN) was higher than that between pure CNFs (202 nN), verifying that CL enhanced the mechanical properties of composite films. In summary, this work constructs a super-strong network between CL and CNF synergistically mediated by metal ion crosslinking and hydrogen bonding, which can be a promising alternative to replace conventional plastics in multiple areas.

Negative parenting style and depression in adolescents: A moderated mediation of self-esteem and perceived social support.

BACKGROUND: Negative parenting style as a risk factors of depression has been defined in the previous researches. However, the underlying mechanism between negative parenting style and depression was still unclear. This study aimed to investigate the mediating role of self-esteem and the moderating role of perceived social support in the association between negative parenting style and depression among adolescents. METHODS: A total of 14,724 Chinese adolescents were asked to complete the questionnaires including Parenting Style scale, Multidimensional Scale of Perceived Social Support, Self-esteem scale, and Patient Health Questionnaire 9-item scale. Mediation and moderation analyses were carried out in SPSS 25.0 macro PROCESS. RESULTS: Self-esteem mediated the relationship between negative parenting styles and adolescent depression (ß = 0.113, SE = 0.004, p < 0.001). Perceived social support moderated the direct effect of negative parenting style on depression (ß = -0.076, SE = 0.009, p < 0.001). Moreover, perceived social support moderated the indirect effect of negative parenting style on self-esteem (ß = -0.023, SE = 0.007, p < 0.001) and the indirect effect of self-esteem on depression (ß = 0.070, SE = 0.009, p < 0.001) in the moderated mediation model. LIMITATIONS: Cross-sectional research design was used in the study. All measures were based on participant self-report. CONCLUSION: This study reveals the underlying mechanism with regard to the influence of negative parenting style on depression through self-esteem and perceived social support. Findings provide a theoretical basis and practical implications for prevention and intervention programs to protect adolescents' mental health.

Characteristics of microplastic-derived dissolved organic matter and its binding with pharmaceuticals unveiled by fluorescence spectroscopy and two-dimensional correlation spectroscopy.

Microplastics were an extensively detected pollutant in the environment, but microplastic-derived dissolved organic matter (MP-DOM) has received less attention, much less its impact on the binding behavior of pollutants (e.g., pharmaceuticals). In this study, DOM derived from two typical MPs, i.e., polyethylene terephthalate (PET) and polystyrene (PS) was generated by UV irradiation (a widely used way for MPs' aging treatment) and characterized by multiple spectroscopic techniques and methods. Chloramphenicol (CAP) and carbamazepine (CBZ) were selected to investigate the binding mechanism between MP-DOM and pharmaceuticals. After UV irradiation, the concentration of the dissolved organic carbon, colored DOM, and carboxyl/carbonyl groups of MP-DOM increased. Moreover, the humic-like substance released preceding and more under UV irradiation. Furthermore, the protein-like substances on PET-DOM and the humic-like substances on PS-DOM were positively correlated to the binding capacity to the pharmaceuticals, respectively. 2D-COS results revealed that the fluorescent materials having more oxygen-containing functional groups for MP-DOM preferentially interacted with the pharmaceuticals. Overall, the higher fluorescence quenching was related to the protein-like substance, CBZ, and PET-DOM as compared to the humic-like substance, CAP, and PS-DOM. It was verified by the relatively high binding ability (logKM) for them (the protein-like substance: 5.15; CBZ: 4.61; PET: 4.48). This study first proved the environmental reactivity of MP-DOM to the pharmaceuticals highlighting the significance of the spectral properties for the binding behavior of MP-DOM with pharmaceuticals and the competitive sorption role of MP-DOM to the pollutants in the natural environment.

Effect of heat treatment on the release of off-flavor compounds in soy protein isolate.

The effects of different heat treatment conditions (65℃ for 30 min, 75℃ for 15 min, and 95℃ for 2, 15 and 30 min) on the evolution of off-flavor compounds in soy protein isolate (SPI) were investigated in terms of lipid oxidation, Maillard reaction and protein structural characteristics. Higher off-flavor concentrations were observed in control and 65℃ treated SPI due to lipoxygenase-mediated enzymatic lipid oxidation. Protein structure played an important role in the release of off-flavors above 65℃. When heated from 75℃ to 95℃ for 2 min, Maillard reaction occurred, glycinin was completely denatured, the particle size increased and the small molecular weight soluble aggregates were formed, resulting in an increase in the content of partial off-flavors. The off-flavor content decreased with time at 95℃, accompanied by the formation of larger molecular weight soluble aggregates. This finding provides practical implications for the beany removal through the SPI structural regulation.

Estimating changes in inequality of ecosystem services provided by green exposure: From a human health perspective.

Ecosystem services provided by green spaces are closely related to human health, strongly supporting sustainable urban and territorial development. Urbanization has not only resulted in the reduction of green spaces but has also created inequalities in exposure. Inequitable green exposure creates disparities in residents' access to the ecosystem services provided by green spaces and can lead to significant health inequities. In this context, we first categorized green exposures into active and passive types based on their characteristics. Second, utilizing the benefit transfer method and Gini coefficient, we estimated the value and equity of ecosystem services offered by these green exposures around residences at the municipality level in Japan from 2000 to 2020, with a focus on human health implications. Finally, we explored the potential relationship between socioeconomics and ecosystem service inequity. Our findings reveal that: 1) ecosystem service value per capita and equity provided by green exposure are significantly different across municipalities; 2) although most municipalities show an upward trend in per capita ecosystem service value around residences, ecosystem service inequity increases significantly; and 3) ecosystem service inequity is related to the socioeconomic factors of municipalities and could be non-linear. The results of this study suggest that the government should adopt indicators related to the ecosystem services provided by green exposure during urban planning. While focusing on per-capita ecosystem services, they should also consider the equitable distribution of ecosystem services to promote sustainable urban health development.

A facile fluorescence microplate immunoassay based on an in situ fluorogenic reaction for the detection of two highly toxic anticoagulant rodenticides in food and biological matrix.

Bromadiolone and brodifacoum, the most frequently used anticoagulant rodenticides, are highly toxic and pose a threat to public health by causing food poisoning incidents. Here, we developed a fluorescence microplate immunoassay for facile and sensitive detection of bromadiolone and brodifacoum by introducing three commercial chemicals (p-phenylenediamine, polyethyleneimine, H2O2) as a new substrate of horseradish peroxidase and then generating fluorescence signals based on an in situ fluorogenic reaction (detection time within 75 min). This assay exhibited higher efficiency in generating fluorescence signals, thereby exhibiting a 6-fold improvement in sensitivity compared with colorimetric ELISA. The limit of detection was 0.23-0.28 ng/mL (ng/g) for bromadiolone and 0.34-0.61 ng/mL (ng/g) for brodifacoum in corn and human serum, with recovery ratios higher than 82.3 %. These satisfactory results illustrated our proposed assay was a potential tool for food analysis and poisoning diagnosis caused by bromadiolone and brodifacoum.

Pyrazines in food samples: Recent update on occurrence, formation, sampling, pretreatment and analysis methods.

Pyrazines are a class of active aromatic substances existing in various foods. The accumulation of pyrazines has an impact on flavor and quality of food products. This review encompasses the formation mechanisms and control strategies of pyrazines via Maillard reaction (MR), including the new reactants and emerging techniques. Pyrazines characteristics are better understood through the developed sample pretreatments and detection methods. Herein, an in-depth review of pretreatments and analysis methods since 2010 is presented to explore the simple, fast, green, and effective strategies. Sample preparation methods include liquid phase extraction, solid phase extraction, supercritical fluid extraction, and microextraction methods such as liquid phase microextraction, and solid phase microextraction, etc. Detections are made by chromatographic methods, and sensors, etc. Advantages and limitations are discussed and compared for providing insights to further studies.

Research progress on generation, detection and inhibition of multiple hazards - acrylamide, 5-hydroxymethylfurfural, advanced glycation end products, methylimidazole - in baked goods.

While baking produces attractive flavors for foods, it also generates various endogenous by-products, including acrylamide (AA), 5-hydroxymethylfurfural (5-HMF), advanced glycation end products (AGEs) and methylimidazole (MI). This review briefly presents the recent studies on the above hazards, and research progress on the formation and control of the above substances in detail. There have been more detailed studies on a single category of hazards. However, few studies and reports have considered the integrated prevention and control of multiple hazards, which is related to the difficulty of analyzing the reaction mechanisms of multiple hazards at multiple scales and under multiple phases in complex food matrices. In this regard, the sample pretreatment methods are a crucial step in achieving simultaneous detection. The coordinated implementation of various methods, including reducing precursor levels, modifying baking conditions and equipment, and incorporating exogenous additives, is necessary to achieve a synchronized reduction in multiple hazardous substances.

Vitamin A deficiency suppresses CEACAM1 to impair colonic epithelial barrier function via downregulating microbial-derived short-chain fatty acids.

Vitamin A (VA) plays an essential role in modulating both the gut microbiota and gut barrier function. Short-chain fatty acids (SCFAs), as metabolites of the gut microbiota, protect the physiological intestinal barrier; however, they are compromised when VA is deficient. Thus, there is an urgent need to understand how and which SCFAs modulate colonic epithelial barrier integrity in VA deficiency (VAD). Herein, compared with normal VA rats (VAN), at the beginning of pregnancy, we confirmed that the colonic desmosome junction was impaired in the VAD group, and the amounts of acetate, propionate, and butyrate declined because of the decreased abundance of SCFA-producing bacteria (Romboutsia, Collinsella, and Allobaculum). The differentially expressed genes correlated with the gut barrier and the histone deacetylase complex between the VAD and VAN groups were enriched by RNA sequencing. In the VAD group, the expression levels of colonic CEA cell adhesion molecule 1 (CEACAM1) were down-regulated, and the levels of histone deacetylase 1 (HDAC1) and HDAC3 were up-regulated. Intriguingly, the above changes in the VAD groups were rescued by VA supplementation in the early postnatal period. Further study indicated that in Caco-2 cells, butyrate treatment significantly repressed the enrichment of HDAC3 on the promoter of the CEACAM1 gene to induce its expression. Our findings support that butyrate intervention can alleviate the impairment of colonic barrier function caused by VAD, and timely postnatal VA intervention may reverse the damage caused by VAD on gut barrier integrity during pregnancy.

Microbial interactions and dynamic changes of volatile flavor compounds during the fermentation of traditional kombucha.

This study aims to explore the core microbiota of kombucha and to discover potential correlations between microbiota and volatile flavor compounds. The total acidity and microbial colony numbers changed dramatically in different fermentation periods of kombucha. Microbial analysis based on high throughput sequencing technology showed that the bacteria of Komagataeibacter, Pseudomonas, Burkholderia, Ralstonia, Halomonas, Sphingomonas and fungi of Dekkera, Saccharomyces cerevisiae, Botryotrichum, Monascus, Pichia were the dominant genera. In addition, the correlation coefficients between the bacteria and fungi were different. The volatile flavor compounds of alcohols, acids, esters, aldehydes, ketones, phenolics, and terpenes were identified using headspace solid-phase microextraction combined with gas chromatography coupled with mass spectrometry. Typically, the concentrations of ethanol, acetic acid, and ethyl acetate was 71.59-248.23 µg/L, 97.73-849.00 µg/L, and 44.52-181.59 µg/L, respectively, during fermentation. This study is helpful to understand the dynamic changes of microbial communities and volatile flavor compounds during the fermentation of kombucha.

Clinical characteristics and risk factors associated with adverse pregnancy outcomes in patients with gestational hypothyroidism: a case control study.

OBJECTIVE: To synthesize evidence, to summarize the clinical features of patients diagnosed with gestational hypothyroidism (GH) and analyze the risk factors associated with adverse pregnancy outcomes. METHODS: From February 2021 to March 2023, a case control study was conducted on hospitalized 298 patients with GH, and 312 pregnant women without GH who underwent physical examinations. The 312 pregnant women without GH were randomly selected during the same time period. They were respectively allocated into the study group and the control group for further comparison of clinical characteristics and pregnancy outcomes. RESULTS: There were significant differences in parameters including age, gestational diabetes, gestational hypertension, gravidity, parity, spontaneous abortion, history of gestation, thyroid stimulating hormone (TSH), free triiodothyronine (FT3), Thyroid peroxidase antibody (TPO-Ab), and free thyroxine (FT4) differ significantly between the two groups (P<0.05). Moreover, significant differences were found between the two groups in terms of preterm delivery, preeclampsia, premature rupture of membranes, placental abruption, and postpartum hemorrhage (P<0.05). The multivariate logistic regression analysis revealed that the influencing factors of pregnancy outcome in patients with GH were age (≥ 30 years old), gestational diabetes, gestational hypertension, gravidity (≥ 3 times), spontaneous abortion, parity, history of gestation (multiparity), and TPO-Ab (positive). CONCLUSION: Our study indeed revealed that the clinical features of patients with GH were age, gestational diabetes, gestational hypertension, gravidity, parity, spontaneous abortion, history of gestation, TSH, FT3, TPO-Ab, and FT4.

Long-term outcomes of surgical interventions for stress urinary incontinence: A systematic review and network meta-analysis.

BACKGROUND: Stress urinary incontinence (SUI) is common among women, and surgical interventions have significantly improved patients' symptoms. The long-term effectiveness of these surgeries is increasingly drawing attention, yet it remains sparsely documented in the literature. OBJECTIVE: To compare the long-term effectiveness and safety of retropubic tension-free vaginal tape (TVT-RP), tension-free vaginal tape-obturator (TVT-O), transobturator tape (TOT), single-incision sling (SIS), Burch colposuspension, and pubovaginal sling (PVS). METHODS: A comprehensive and systematic literature review was conducted in PubMed, EMBASE, MEDLINE, Cochrane Library, Medicine, and clinicaltrials.gov from inception to May 2023. Selected trials were evaluated for potential bias using the Cochrane tool. Treatment modalities were compared using network meta-analysis to assess objective success rate, subjective success rate, and complications as outcomes. RESULTS: A total of 37 studies involving 5,720 patients were included. No significant statistical differences were found among the interventions regarding objective success rate. PVS had the highest surface under the cumulative ranking curve SUCRA value (93.1). For subjective success rate, TVT-RP, TVT-O, and PVS demonstrated superiority over SIS, with PVS having the highest SUCRA value (80.1). SIS had lower overall complication and pain rates compared to other methods, with statistical significance. There were no differences in reoperation rate, exposure rate, and urinary tract infection occurrence among the surgical approaches. CONCLUSIONS: In terms of long-term effectiveness and safety, TVT-RP and TVT-O appear to be the preferred options for patients opting for synthetic slings, while for patients seeking non-synthetic slings, PVS may represent the optimal choice.

Effects of maternal pulmonary arterial hypertension on fetal hemodynamics and maternal-fetal outcome in late pregnancy.

PURPOSE: The aim of this study was to investigate the effects of maternal pulmonary arterial hypertension (PAH) on fetal hemodynamics in the third trimester and to identify hemodynamic indicators associated with adverse maternal and fetal outcomes. METHODS: We recruited 48 pregnant women with PAH in the third trimester and 32 women with normal pregnancies as controls matched for age and gestational week. Fetal growth and hemodynamic parameters were assessed by two-dimensional and color Doppler. All cases were followed up until delivery and maternal and fetal outcomes were collected. High throughput sequencing method was used to determine differential miRNA patterns in plasma exposed to pulmonary arterial hypertension (PAH) in pregnant women. We then performed the validated of key differentially expressed miRNAs by real-time PCR. RESULTS: Compared with the normal and mild PAH groups, resistance index (RI), pulsatility index (PI) of the fetal umbilical artery (UA) and quantitative ductus venosus (QDV) blood flow were increased in subjects with moderate to severe PAH, while PI and the ratio of peak systolic velocity (PSV) to end-diastolic velocity (EDV) (S/D) of the middle cerebral artery (MCA) were decreased. Compared with the normal group, subjects in the mild and moderate PAH groups had lower neonatal weight, shorter neonatal height, and higher preterm birth rates. In addition, miRNA sequencing data showed that PAH affected the levels of 23 miRNAs in plasma. At the same time, we showed that PAH significantly decreased the level of miR-1255a and increased the level of miR-548ar-3p by real-time PCR. CONCLUSION: In the group of pregnant women with moderate to severe pulmonary hypertension, there was a higher proportion of preterm births and low birth weight babies. Hemodynamic changes in the fetal UA, MCA, and ductus venosus (DV) during late pregnancy were associated with adverse fetal outcomes. At the same time, miRNA sequencing results showed that miR-1255a and miR-548ar-3p may play an important role in the development of PAH.

Successful therapy using high-dose furmonertinib for non-small cell lung cancer with leptomeningeal metastasis: a case report and literature review.

Background: Lung cancer is the second most common form of malignant tumor and has the highest mortality rate worldwide. Among its subtypes, lung adenocarcinoma is the most prevalent. Leptomeningeal metastasis (LM) is rare and is characterized by a dismal prognosis, with overall survival periods typically spanning 4 to 6 weeks without treatment. However, in specific cases, survival can be extended to 4 to 6 months with appropriate therapy. The recent approval of third-generation tyrosine kinase inhibitors (TKIs), such as osimertinib, aumolertinib, and furmonertinib, has introduced promising treatment options for individuals with non-small cell lung cancer (NSCLC) who develop LM after developing resistance to first- and second-generation TKIs. These third-generation TKIs exhibit an enhanced ability to penetrate the blood-brain barrier (BBB), opening up new avenues for managing this challenging condition. Case summary: We report the case of a 48-year-old Chinese man diagnosed with advanced NSCLC harboring an epidermal growth factor receptor (EGFR) mutation. Following a pulmonary lobectomy and postoperative adjuvant therapy with gefitinib, the patient was diagnosed with LM, which was confirmed by his neurologic symptoms, cerebrospinal fluid cytologic analysis, and cranial enhancement magnetic resonance imaging. Subsequently, he received oral treatment in the form of 160 mg of furmonertinib daily. After 5 days of furmonertinib therapy, the patient recovered from lethargy, with an obvious improvement in cognitive function. Follow-up visits revealed a 6-month survival period following the LM diagnosis. Patients with NSCLC and LM typically present with severe symptoms, and the efficacy of systemic treatment, intrathecal chemotherapy, and radiotherapy remains unsatisfactory. We hope that this specific case provide valuable insights into the management of patients with EGFR mutation-associated NSCLC with LM. Conclusion: Furmonertinib, a third-generation EGFR TKI with notable BBB penetration, shows promise in LM control and the rapid alleviation of intracranial symptoms. Further investigations into appropriate dosage and toxicity management are imperative.

Impacts of morphology parameters on the risk of rupture in intracranial aneurysms: statistical and computational analyses.

The hemodynamic analysis of the blood stream inside the cerebral aneurysms reveals the risk of the aneurysm rupture. In addition, the high risk region prone to rupture would be determined by the hemodynamic analysis of the blood. In present article, computational fluid dynamic is used for the investigation of the hemodynamic effects on the aneurysm wall and risk of rupture. This study tries to find the connection between the risk of rupture with three geometrical features of aneurysm i.e., Ellipsoid Max semi-axis, Size ratio and Tortuosity. Statistical analysis is done over 30 different ruptured /unruptured ICA aneurysms to find meaningful relation between selected geometrical factors and rupture risk. The hemodynamic analysis is done over four distinct aneurysm models to attain more details on effects of chosen geometrical factors. The results of simulations indicate that the Ellipsoid Max semi-axis have meaningful impacts on the risk of rupture.

Effectiveness of group acceptance and commitment therapy in treating depression for acute stroke patients.

OBJECTIVES: To date, the effectiveness of acceptance and commitment therapy (ACT) for acute stroke patients has not been well recognized. The study aimed to discover the effectiveness of group-based ACT in treating depression for acute stroke patients. METHODS: We conducted a randomized controlled trial with 140 acute stroke patients with depression. The ACT intervention comprised seven sessions, of 45-60 min over 4 weeks. Data were collected pre- and post-intervention and at 3-month follow-up, assessing depression, health-related quality of life (HRQoL), psychological flexibility, cognitive fusion, sleep quality, and confidence. RESULTS: Overall, 99.3% of the included patients were assessed as having mild depression. The ACT intervention significantly reduced depression in acute stroke patients in comparison with the control group post-intervention and at 3 months (partial η 2 = . 306 $\eta^{2}=.306$ ). Additionally ACT significantly improved HRQoL-mental component summary, sleep quality, psychological flexibility, cognitive fusion, and confidence compared with control group. CONCLUSIONS: ACT is effective in treating acute stroke patients with depression, and the efficacy was maintained at 3-month follow-up.

Multiple sequence alignment-based RNA language model and its application to structural inference.

Compared with proteins, DNA and RNA are more difficult languages to interpret because four-letter coded DNA/RNA sequences have less information content than 20-letter coded protein sequences. While BERT (Bidirectional Encoder Representations from Transformers)-like language models have been developed for RNA, they are ineffective at capturing the evolutionary information from homologous sequences because unlike proteins, RNA sequences are less conserved. Here, we have developed an unsupervised multiple sequence alignment-based RNA language model (RNA-MSM) by utilizing homologous sequences from an automatic pipeline, RNAcmap, as it can provide significantly more homologous sequences than manually annotated Rfam. We demonstrate that the resulting unsupervised, two-dimensional attention maps and one-dimensional embeddings from RNA-MSM contain structural information. In fact, they can be directly mapped with high accuracy to 2D base pairing probabilities and 1D solvent accessibilities, respectively. Further fine-tuning led to significantly improved performance on these two downstream tasks compared with existing state-of-the-art techniques including SPOT-RNA2 and RNAsnap2. By comparison, RNA-FM, a BERT-based RNA language model, performs worse than one-hot encoding with its embedding in base pair and solvent-accessible surface area prediction. We anticipate that the pre-trained RNA-MSM model can be fine-tuned on many other tasks related to RNA structure and function.

Nocebo effects and influencing factors in the randomized clinical trials of chronic constipation: A systematic review and meta-analysis.

BACKGROUND: Nocebo effects are unavoidable in randomized clinical trials. We aimed to assess the magnitude of nocebo effects and explore the influencing factors in chronic constipation. METHODS: We searched the PubMed, Embase, and Cochrane Library databases up to July 2022. Randomized, placebo-controlled trials investigating interventions in chronic constipation were included. We conducted a random effects meta-analysis of the proportion of adverse events (AEs) in placebo-treated participants and evaluated the effect of trial characteristics on nocebo effects. KEY RESULTS: We identified 20,204 studies from the databases, of which 61 were included in the final analysis. The pooled placebo AE rate was 30.41%, and AE-related withdrawal rate was 1.53%. The most commonly reported AEs were headache (5.67%), diarrhea (4.45%), abdominal pain (3.98%), nasopharyngitis (3.39%), nausea (3.36%), and flatulence (2.95%). The placebo AE rate was lower in trials conducted in Asia compared to those in Europe, North America, and international trials. It was also lower in trials diagnosed by Rome III compared to clinician's opinion and Rome II. Additionally, the placebo AE rate was lower in single-center trials compared to multicenter trials, lower in 5-8 weeks therapy compared to 9-12 weeks therapy, lower in participants with FC compared to those with IBS-C and CC, lower in trials with 2 arms compared to 3 arms, and higher in trials with prokinetic drugs compared to secretagogues and laxatives. CONCLUSIONS & INFERENCES: The placebo AE rate was 30.41% in patients with chronic constipation. Based on our findings, we recommend that researchers take the nocebo effects into consideration when designing and conducting clinical trials and adopt specific measures to mitigate the negative influence of nocebo effects.

Comparison of stem cell transplantation using unrelated, haploidentical, and sibling donors for patients with acquired severe aplastic anemia : A single center, retrospective, cohort study.

The preferred donor (haploidentical vs. matched unrelated donor) choice in patients with acquired severe aplastic anemia (SAA) who lack a human leukocyte antigen (HLA)-matched sibling donors (MSDs) and fail upfront immunosuppressive treatment (IST) therapy is unknown. We retrospectively investigated SAA patients (n=58) who underwent allogeneic stem cell transplantation (allo-SCT) between January 2012 and October 2022. The 5-year overall survival (OS) (87.5% ± 11.7% vs. 98.0% ± 6.5% vs. 83.3% ± 7.6%, respectively, p= 0.926) and 5-year failure-free survival (FFS) (60.0% ± 18.2% vs. 87.0% ± 7.0% vs. 78.3% ± 8.6%, respectively, p= 0.222) were comparable among the unrelated donors (URD; n=8), haploidentical donors (HID; n=25), and MSD cohorts (n=25). Multivariate analysis revealed that primary engraftment failure independently predicted OS, and secondary graft failure predicted FFS among SAA patients who underwent allo-SCT, although donor type and age did not predict the outcomes. An urgent second SCT for patients with engraftment failure may be an effective salvage treatment. Summarily, our findings showed that an alternative donor SCT is indicated for eligible SAA patients without MSDs even if these patients are aged ⩾ 40 years.

Molecularly Engineered Room-Temperature Phosphorescence for Biomedical Application: From the Visible toward Second Near-Infrared Window.

Phosphorescence, characterized by luminescent lifetimes significantly longer than that of biological autofluorescence under ambient environment, is of great value for biomedical applications. Academic evidence of fluorescence imaging indicates that virtually all imaging metrics (sensitivity, resolution, and penetration depths) are improved when progressing into longer wavelength regions, especially the recently reported second near-infrared (NIR-II, 1000-1700 nm) window. Although the emission wavelength of probes does matter, it is not clear whether the guideline of "the longer the wavelength, the better the imaging effect" is still suitable for developing phosphorescent probes. For tissue-specific bioimaging, long-lived probes, even if they emit visible phosphorescence, enable accurate visualization of large deep tissues. For studies dealing with bioimaging of tiny biological architectures or dynamic physiopathological activities, the prerequisite is rigorous planning of long-wavelength phosphorescence, being aware of the cooperative contribution of long wavelengths and long lifetimes for improving the spatiotemporal resolution, penetration depth, and sensitivity of bioimaging. In this Review, emerging molecular engineering methods of room-temperature phosphorescence are discussed through the lens of photophysical mechanisms. We highlight the roles of phosphorescence with emission from visible to NIR-II windows toward bioapplications. To appreciate such advances, challenges and prospects in rapidly growing studies of room-temperature phosphorescence are described.