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1.
Harmful Algae ; 111: 102152, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35016765

RESUMO

Marine phycotoxins associated with paralytic shellfish poisoning (PSP), diarrhetic shellfish poisoning (DSP), amnesic shellfish poisoning (ASP), neurotoxic shellfish poisoning (NSP), ciguatera fish poisoning (CFP), tetrodotoxin (TTX), palytoxin (PLTX) and neurotoxin ß-N-methylamino-L-alanine (BMAA) have been investigated and routinely monitored along the coast of China. The mouse bioassay for monitoring of marine toxins has been progressively replaced by the enzyme-linked immunosorbent assay (ELISA) and liquid chromatography tandem mass spectrometry (LC-MS/MS), which led to the discovery of many new hydrophilic and lipophilic marine toxins. PSP toxins have been detected in the whole of coastal waters of China, where they are the most serious marine toxins. PSP events in the Northern Yellow Sea, the Bohai Sea and the East China Sea are a cause of severe public health concern. Okadaic acid (OA) and dinophysistoxin-1 (DTX1), which are major toxin components associated with DSP, were mainly found in coastal waters of Zhejiang and Fujian provinces, and other lipophilic toxins, such as pectenotoxins, yessotoxins, azaspiracids, cyclic imines, and dinophysistoxin-2(DTX2) were detected in bivalves, seawater, sediment, as well as phytoplankton. CFP events mainly occurred in the South China Sea, while TTX events mainly occurred in Jiangsu, Zhejiang and Fujian provinces. Microalgae that produce PLTX and BMAA were found in the phytoplankton community along the coastal waters of China.

2.
Aging (Albany NY) ; 14(1): 368-388, 2022 01 06.
Artigo em Inglês | MEDLINE | ID: mdl-34990401

RESUMO

Hepatocellular carcinoma (HCC) is one of the most death-related cancers worldwide. Identifying cancer-associated genes and uncovering the vital molecular mechanisms of HCC progression contribute greatly to the prognosis and novel therapeutic strategies for HCC patients. Although lncRNAs have been proved to be critical modulators of various cellular processes, the functions of lncRNAs in HCC progression are just emerging. Here, we found that a long non-coding RNA (lncRNA) named LINC02362, whose biological effects have yet been unveiled in cancers, was associated with a better prognosis in patients with HCC. Gain-of-function analyses showed that LINC02362 inhibited the survival, migration, invasion and epithelial-to-mesenchymal transition (EMT) of HCC cells. Moreover, miR-516b-5p was enriched as a target of LINC02362, which functioned as a sponge to regulate the endogenous levels of miR-516b-5p. Furthermore, we confirmed that SOSC2 served as a downstream target gene which was negatively controlled by miR-516b-5p. Importantly, a series of rescue experiments indicated that the tumor-suppressive effects of LINC02362 were achieved through the modulation of the miR-516b-5p/SOSC2 axis. In summary, we identified LINC02362 as a candidate tumor-inhibitory lncRNA that might serve as a biomarker for the prognosis of HCC and a promising therapeutic agent for patients with HCC.

3.
J Environ Sci (China) ; 113: 40-54, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34963548

RESUMO

Ambient carbonyls were continuously observed in the field during a heavy ozone pollution episode in Chengdu, China from August 4 to August 19, 2019, and the pollution characteristics, atmospheric photochemical reactivity, human health risk, and sources of carbonyls were analyzed. Fifteen carbonyls were quantified with average total mixing ratios of 20.38 ppbv Formaldehyde (9.86 ppbv), acetone (4.41 ppbv), and acetaldehyde (3.57 ppbv) were the three most abundant carbonyls. During the heavy ozone pollution episode, the concentration of carbonyls was found to be higher on pollution days than on the clean days, and relatively higher in the daytime, especially at noon on the pollution days. This was influenced by the intensity of photochemical reactions and precipitation. The "weekend effect" with the concentration of carbonyls was higher on the weekends than on the weekdays was pointed out. Formaldehyde, acetaldehyde and hexaldehyde were the dominant oxidative species during the observation. The carcinogenic and non-carcinogenic risk values of formaldehyde and acetaldehyde were higher on pollution days than on clean days, and these values were higher compared with those of other cities in China and abroad. Long-term exposure to these compounds should therefore be avoided. Diagnostic ratios and correlation analysis together with backward trajectory analysis showed that primary emission and secondary formation accounted 66%-76% and 24%-34% of carbonyls in Chengdu, respectively, with primary emission being the main sources of carbonyls, and carbonyls from the surrounding cities and emission from natural sources also had a significant contribution to the carbonyls in Chengdu.


Assuntos
Poluentes Atmosféricos , Ozônio , Compostos Orgânicos Voláteis , Poluentes Atmosféricos/análise , China , Monitoramento Ambiental , Humanos , Ozônio/análise , Medição de Risco , Compostos Orgânicos Voláteis/análise
4.
Exp Ther Med ; 23(2): 126, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34970349

RESUMO

Traumatic brain injury (TBI) has been recognized as a serious public health issue and a key contributor to disability and death, with a huge economic burden worldwide. Hydrogen, which is a slight and specific cytotoxic oxygen radical scavenger, has been demonstrated to ameliorate early brain injury (EBI) through reactive oxygen species (ROS), oxidative stress injury, apoptosis and necroptosis. Necroptosis refers to a type of programmed cell death process that has a vital function in neuronal cell death following TBI. The specific function of necroptosis in hydrogen-mediated neuroprotection after TBI, however, has yet to be determined. The present study aimed to examine the neuroprotective effects and possible molecular basis that underly hydrogen-rich saline in TBI-stimulated EBI by examining neural necroptosis in the C57BL/6 mouse model. The brain water content, neurological score, neuroinflammatory cytokines (NF-κΒ, TNF-α, IL-6 and IL-1ß) and ROS were evaluated using flow cytometry. Malondialdehyde, superoxide dismutase (SOD) and glutathione (GSH) levels were evaluated using a biochemical kit. Receptor-interacting protein kinase (RIP)1, RIP3, Nrf2 and Heme oxygenase-1 (HO-1) were evaluated using western blotting. mRNA of Nrf2 and HO-1 were evaluated using quantitative PCR. Neuronal death was evaluated by TUNEL staining. The outcomes illustrated that hydrogen-rich saline treatment considerably enhanced the neurological score, increased neuronal survival, decreased the levels of serum MDA and brain ROS, increased the levels of serum GSH and SOD. In addition the protein expression levels of RIP1 and RIP3 and the cytokines NF-κB, TNF-α, IL-1ß and IL-6 were downregulated compared with the TBI group, which demonstrated that hydrogen-rich saline-induced inhibition of necroptosis and neuroinflammation ameliorated neuronal death following TBI. The neuroprotective capacity of hydrogen-rich saline was demonstrated to be partly dependent on the ROS/heme oxygenase-1 signaling pathway. Taken together, the findings of the present study indicated that hydrogen-rich saline enhanced neurological outcomes in mice and minimized neuronal death by inducing protective effects against neural necroptosis as well as neuroinflammation.

5.
Virol J ; 18(1): 250, 2021 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-34906167

RESUMO

BACKGROUND: Daphnis nerii cypovirus-23 (DnCPV-23) is a new type of cypovirus and has a lethal effect on the oleander hawk moth, Daphnis nerii which feeds on leave of Oleander and Catharanthus et al. After DnCPV-23 infection, the change of Daphnis nerii responses has not been reported. METHODS: To better understand the pathogenic mechanism of DnCPV-23 infection, 3rd-instar Daphnis nerii larvae were orally infected with DnCPV-23 occlusion bodies and the transcriptional responses of the Daphnis nerii midgut were analyzed 72 h post-infection using RNA-seq. RESULTS: The results showed that 1979 differentially expressed Daphnis nerii transcripts in the infected midgut had been identified. KEGG analysis showed that protein digestion and absorption, Toll and Imd signaling pathway were down-regulated. Based on the result, we speculated that food digestion and absorption in insect midgut might be impaired after virus infection. In addition, the down-regulation of the immune response may make D. nerii more susceptible to bacterial infections. Glycerophospholipid metabolism and xenobiotics metabolism were up-regulated. These two types of pathways may affect the viral replication and xenobiotic detoxification of insect, respectively. CONCLUSION: These results may facilitate a better understanding of the changes in Daphnis nerii metabolism during cypovirus infection and serve as a basis for future research on the molecular mechanism of DnCPV-23 invasion.

6.
Front Neurosci ; 15: 755709, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34744616

RESUMO

Objective: Intermittent theta burst stimulation (iTBS) is a special form of repetitive transcranial magnetic stimulation (rTMS), which effectively increases cortical excitability and has been widely used as a neural modulation approach in stroke rehabilitation. As effects of iTBS are typically investigated by motor evoked potentials, how iTBS influences functional brain network following stroke remains unclear. Resting-state electroencephalography (EEG) has been suggested to be a sensitive measure for evaluating effects of rTMS on brain functional activity and network. Here, we used resting-state EEG to investigate the effects of iTBS on functional brain network in stroke survivors. Methods: We studied thirty stroke survivors (age: 63.1 ± 12.1 years; chronicity: 4.0 ± 3.8 months; UE FMA: 26.6 ± 19.4/66) with upper limb motor dysfunction. Stroke survivors were randomly divided into two groups receiving either Active or Sham iTBS over the ipsilesional primary motor cortex. Resting-state EEG was recorded at baseline and immediately after iTBS to assess the effects of iTBS on functional brain network. Results: Delta and theta bands interhemispheric functional connectivity were significantly increased after Active iTBS (P = 0.038 and 0.011, respectively), but were not significantly changed after Sham iTBS (P = 0.327 and 0.342, respectively). Delta and beta bands global efficiency were also significantly increased after Active iTBS (P = 0.013 and 0.0003, respectively), but not after Sham iTBS (P = 0.586 and 0.954, respectively). Conclusion: This is the first study that used EEG to investigate the acute neuroplastic changes after iTBS following stroke. Our findings for the first time provide evidence that iTBS modulates brain network functioning in stroke survivors. Acute increase in interhemispheric functional connectivity and global efficiency after iTBS suggest that iTBS has the potential to normalize brain network functioning following stroke, which can be utilized in stroke rehabilitation.

7.
ACS Omega ; 6(45): 30526-30533, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34805681

RESUMO

It is of great importance to understand the thermal properties of MoSe2 films for electronic and optoelectronic applications. In this work, large-area polycrystalline MoSe2 films are prepared using a low-cost, controllable, large-scale, and repeatable chemical vapor deposition method, which facilitates direct device fabrication. Raman spectra and X-ray diffraction patterns indicate a hexagonal (2H) crystal structure of the MoSe2 film. Ellipsometric spectra analysis indicates that the optical band gap of the MoSe2 film is estimated to be ∼1.23 eV. From the analysis of the temperature-dependent and laser-power-dependent Raman spectra, the thermal conductivity of the suspended MoSe2 films is found to be ∼28.48 W/(m·K) at room temperature. The results can provide useful guidance for an effective thermal management of large-area polycrystalline MoSe2-based electronic and optoelectronic devices.

8.
Eur J Pharmacol ; 912: 174550, 2021 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-34610279

RESUMO

BACKGROUND: The interaction between tumor cells and tumor microenvironment is a necessary condition for promoting the metastasis of malignant tumors. METHODS: Two different transwell culture systems were interfered with by recombinant factor placental growth factor (re-PIGF) and the re-PIGF + transforming growth factor-ß1 (TGF-ß1)-neutralizing antibody (anti-TGF-ß1). We performed immunofluorescence, flow cytometry and enzyme linked immunosorbent assay (ELISA) to analyze the expression of PIGF, fms-like tyrosine kinase-1 (Flt-1), macrophage marker F4/80 +, macrophage M2 marker CD163+ and TGF-ß1 in vitro. Meanwhile, cell viability assay and optical microscope assay were conducted to explore the cell viability and vascularization ability of human umbilical vein endothelial cells (HUVECs). RESULTS: Re-PIGF increased the expression of PIGF in A549 cells and the expression of Flt-1 in BM-Mac cells, and significantly enhanced the ability of bone marrow-derived macrophages (BM-Mac) to transform into macrophages. At the same time, re-PIGF increased the expression of cytokine TGF-ß1 in A549 cells/BM-Mac transwell culture system. On the contrary, re-PIGF + anti-TGF-ß1 inhibited the expression of Flt-1 in BM-Mac cells and inhibited the ability of BM-Mac cells to transform into macrophages. Finally, re-PIGF + anti-TGF-ß1 reduced the cell viability and angiogenesis of HUVECs. CONCLUSION: The surface molecule PIGF of lung cancer cells could bind to the receptor Flt-1 on the surface of macrophages, thereby increasing the production of TGF-ß1, and ultimately promoting the formation of angiogenesis in lung cancer.

9.
J Trauma Nurs ; 28(5): 298-303, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34491945

RESUMO

BACKGROUND: The high mortality rate of comatose patients with traumatic brain injury is a prominent public health issue that negatively impacts patients and their families. Objective, reliable tools are needed to guide treatment decisions and prioritize resources. OBJECTIVE: This study aimed to evaluate the prognostic value of the bispectral index (BIS) in comatose patients with severe brain injury. METHODS: This was a retrospective cohort study of 84 patients with severe brain injury and Glasgow Coma Scale (GCS) scores of 8 and less treated from January 2015 to June 2017. Sedatives were withheld at least 24 hr before BIS scoring. The BIS value, GCS scores, and Full Outline of UnResponsiveness (FOUR) were monitored hourly for 48 hr. Based on the Glasgow Outcome Scale (GOS) score, the patients were divided into poor (GOS score: 1-2) and good prognosis groups (GOS score: 3-5). The correlation between BIS and prognosis was analyzed by logistic regression, and the receiver operating characteristic curves were plotted. RESULTS: The mean (SD) of the BIS value: 54.63 (11.76), p = .000; and GCS score: 5.76 (1.87), p = .000, were higher in the good prognosis group than in the poor prognosis group. Lower BIS values and GCS scores were correlated with poorer prognosis. Based on the area under the curve of receiver operating characteristic curves, the optimal diagnostic cutoff value of the BIS was 43.6, and the associated sensitivity and specificity were 85.4% and 74.4%, respectively. CONCLUSION: Taken together, our study indicates that BIS had good predictive value on prognosis. These findings suggested that BIS could be used to evaluate the severity and prognosis of severe brain injury.


Assuntos
Lesões Encefálicas , Coma , Coma/diagnóstico , Eletroencefalografia , Escala de Coma de Glasgow , Humanos , Prognóstico , Estudos Retrospectivos
10.
PLoS Genet ; 17(9): e1009760, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34491994

RESUMO

Unisexual taxa are commonly considered short-lived as the absence of meiotic recombination is supposed to accumulate deleterious mutations and hinder the creation of genetic diversity. However, the gynogenetic gibel carp (Carassius gibelio) with high genetic diversity and wide ecological distribution has outlived its predicted extinction time of a strict unisexual reproduction population. Unlike other unisexual vertebrates, males associated with supernumerary microchromosomes have been observed in gibel carp, which provides a unique system to explore the rationales underlying male occurrence in unisexual lineage and evolution of unisexual reproduction. Here, we identified a massively expanded satellite DNA cluster on microchromosomes of hexaploid gibel carp via comparing with the ancestral tetraploid crucian carp (Carassius auratus). Based on the satellite cluster, we developed a method for single chromosomal fluorescence microdissection and isolated three male-specific microchromosomes in a male metaphase cell. Genomic anatomy revealed that these male-specific microchromosomes contained homologous sequences of autosomes and abundant repetitive elements. Significantly, several potential male-specific genes with transcriptional activity were identified, among which four and five genes displayed male-specific and male-biased expression in gonads, respectively, during the developmental period of sex determination. Therefore, the male-specific microchromosomes resembling common features of sex chromosomes may be the main driving force for male occurrence in gynogenetic gibel carp, which sheds new light on the evolution of unisexual reproduction.

11.
Huan Jing Ke Xue ; 42(10): 4611-4620, 2021 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-34581103

RESUMO

To study the characteristics of O3 pollution and identify the key precursors for O3 formation in Chengdu in spring, O3 concentrations in April between 2016 and 2018 were analyzed, and on-line measurements of O3 and the precursors(VOCs and NOx) were also studied at an urban site. The results showed that the O3 pollution level in April increased year by year, and diurnal variations showed a unimodal distribution. When the ambient temperature was more than 20℃, the wind speed was between 1 and 1.5 m·s-1, and the relative humidity was less than 65%, the probability of O3 pollution occurring in April was more than 80%. In April 2018, the average concentrations of NOx and VOCs during O3 pollution days were 2.3-times and 2-times higher than non-pollution days. Furthermore, an OBM method was used to calculate the RIR values of different ozone precursors. This showed that the RIR values of anthropogenic VOCs, CO, biogenic VOCs, and NOx for ozone were 2.4, 0.87, 0.06, and -2.6, respectively, indicating that O3 formation in Chengdu was generally VOC-limited. The RIR values of the VOC species showed that m/p-xylene, ethylene, trans-2-butane, propylene, o-xylene, toluene, acetone, isoprene, isopentane, and n-butane were the key active VOC species of ozone formation.


Assuntos
Poluentes Atmosféricos , Ozônio , Compostos Orgânicos Voláteis , Poluentes Atmosféricos/análise , Monitoramento Ambiental , Ozônio/análise , Estações do Ano , Compostos Orgânicos Voláteis/análise
12.
Environ Pollut ; 291: 118210, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34582920

RESUMO

The occurrence, spatiotemporal variations, influence factors and environmental risks of eight common neonicotinoids (NEOs), fipronil, and three fipronil metabolites (fipronil and its metabolites are collectively referred to as FIPs) in different seasons from the estuary to the inner area of Jiaozhou Bay, China were comprehensively investigated. First- and second-generation NEOs were found to be the predominant pesticides in this area. The average contents of ∑NEOs and ∑FIPs from the estuary to the inner bay decreased from 12.99 ng/L to 0.82 ng/L and from 1.10 ng/L to 0.17 ng/L, respectively. NEO and FIP concentrations were higher in summer and autumn. High ∑NEO content is distributed in main inflow rivers, such as Dagu River and Licun River, which are influenced by pesticide application. NEO concentrations in all rivers were high upstream and low downstream because of the influence of heavy rainfall and seawater dilution in summer. NEO concentrations were high along the coast and low at the mouth and center of Jiaozhou Bay in summer and autumn and evenly distributed in winter and spring. Temperature has a great influence on most NEOs and FIPs owing to its effect on their degradation. Nitrogen-containing nutrients have an important influence on the distribution of fipronil and acetamiprid, which may be due to the activity of nitrogen-containing functional groups in their structure. Only Licun River, Dagu River and Haibo river sewage treatment plant in summer posed a certain risk of chronic toxicity for NEOs using the new threshold established by the species sensitive distribution (SSD) method for Chinese native aquatic lives. These findings should arouse people's attention.


Assuntos
Baías , Poluentes Químicos da Água , China , Monitoramento Ambiental , Humanos , Neonicotinoides/análise , Pirazóis , Poluentes Químicos da Água/análise
13.
J Clin Neurosci ; 91: 118-124, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34373015

RESUMO

OBJECTIVE: Papillary meningioma is rare and displays an aggressive clinical behavior with poor prognosis. Therefore, we performed an extensive literature review to evaluate the adverse factors and treatment strategy of survival. METHOD: We performed Ovid, Medline, Embase, Pubmed, Web of Science and Cochrane database queries for articles published between 1938 and 2019 with the search term "WHO grade III meningioma" or "papillary meningioma" and "central nervous system", "cerebral", or "intracranial". RESULTS: After a careful evaluation, a total of 19 studies were included. The entire cohort included the 67 patients, 34 (50.7%) were male and 33 (49.3%) were female with a mean age of 32.6 ± 2.1 years ranging from 4.5 months to 74 years. Gross total resection was achieved in 48 (71.6%) cases, and 29 (51.8%) patients received postoperative radiation. The mean follow-up period was 42.3 ± 4.4 months (range, 2-197 months). Thirty-six (53.7%) patients happened to recurrences, 11 (16.4%) patients happened to extracranial metastasis and 25 (37.3%) patients died. Univariate analysis revealed that the MIB > 5% trended toward a shorter time to recurrence (p = 0.084). Gross total resection was associated with favorable progression-free survival (p = 0.007) and overall survival (p = 0.001). Postoperative radiation was associated with favorable progression-free survival (p = 0.001). CONCLUSIONS: Gross total resection and adjuvant radiation were recommended as the initial treatment option for patients with papillary meningioma.


Assuntos
Neoplasias Meníngeas , Meningioma , Adulto , Feminino , Humanos , Masculino , Neoplasias Meníngeas/diagnóstico , Neoplasias Meníngeas/terapia , Meningioma/diagnóstico , Meningioma/terapia , Recidiva Local de Neoplasia , Prognóstico , Intervalo Livre de Progressão , Estudos Retrospectivos
14.
Int J Gen Med ; 14: 4405-4411, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34408479

RESUMO

Objective: The purpose of this study is to investigate the risk factors of epilepsy based on the National Health and Nutrition Examination Survey (NHANES). Methods: The data in this study was obtained from the NHANES database between 2013 and 2018. It included 14,290 participants aged between 20 and 80. We defined people with epilepsy (PWE) when they self-reported took at least one treatment medication for seizures or epilepsy. Analysis of risk factors for epilepsy mainly includes Student's t-test, chi-square test, univariate and multivariate logistic regression analysis. Results: People aged 40-59 shared 1.8 times the risk of epilepsy than those who aged 20-39, P=0.034. People who never married had a 2.8-fold higher risk of epilepsy than those who married/living with partner, P<0.001. The risk of epilepsy in subjects with very good/good general health was 0.4 times than that of subjects with fair/poor general health, P<0.001. Moreover, subjects without sleep disorders had a 0.4-fold higher risk of epilepsy than those who had sleep disorders, P=0.042. Conclusion: People who are older, unmarried, and have sleep disorders are at higher risk of epilepsy. In addition, good/good general health condition is associated with a lower risk of epilepsy.

15.
Sci Total Environ ; 801: 149717, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34425443

RESUMO

Despite fresh and pyrogenic organic matter have been widely used as amendments to improve soil organic carbon (SOC) storage, mineralization that links to C quality and soil temperature, microbial community composition and enzyme activity remain poorly understood. This study aims to explore the effects of amendments (bamboo leaves and its biochar) and incubation temperature on mineralization, and disentangle the relationships of SOC mineralization with chemical composition of SOC, labile organic C, microbial community composition, and activities of enzymes in a subtropical bamboo forest soil. Results showed that cumulative soil CO2 emissions ranked as bamboo leaf (Leaf) > bamboo leaf biochar (Biochar) > Control, regardless of the incubation temperature. Compared to the control, the Leaf treatment markedly increased, whereas the Biochar treatment decreased, the temperature sensitivity of SOC mineralization (P < 0.05). The cumulative soil CO2 emission was positively correlated (P < 0.05) with water-soluble organic C (WSOC), microbial biomass C (MBC), O-alkyl C and alkyl C contents, and activities of ß-glucosidase and dehydrogenase, but negatively correlated (P < 0.01) with aromatic C content, regardless of the incubation temperature. This indicated that the lower SOC mineralization rate and lower temperature sensitivity in the Biochar (cf. Leaf) treatment were intimately associated with the lower WSOC, MBC, O-alkyl C content, and ß-glucosidase and dehydrogenase activities, and higher aromatic C content in the Biochar. The high relative abundance of bacteria relating SOC mineralization included Rhizobiales, Sphingobacteriales and JG30-KF-AS9, whereas that of fungi included Eurotiales, Sordariales, Agaricales and Helotiales. Our results revealed that the application of pyrogenic organic matter, as compared to the application of fresh organic matter, can reduce SOC mineralization and its temperature sensitivity in a subtropical forest soil by limiting the availability of C and microbial activity, and thus has a great potential for maintaining soil carbon stock in subtropical forest ecosystems.


Assuntos
Microbiota , Solo , Carbono , Carvão Vegetal , Florestas , Microbiologia do Solo
16.
Ecol Evol ; 11(14): 9765-9775, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34306660

RESUMO

The diamondback moth, Plutella xylostella, is an important agricultural pest that severely damages cruciferous vegetables. Although previously considered a threat only to Brassica species, P. xylostella has been observed to feed on noncruciferous vegetables. Here, we established a population of P. xylostella on the pea Pisum sativum (PxP population). We compared this PxP population's performance on the pea host plant to a population (PxR) reared on the original host plant radish (Raphanus sativus) for several generations using an age-stage, two-sex life table and analyzed the correlations between different fitness parameters. In the 1st generation of the PxP population, survival rate of immature stage was 17%, while the survival rate of PxR was 68%; the duration of the 4th larval instar (5.30 d) and mortality (25%) of this generation were significantly longer (2.8 d) and higher (1%) than that of PxR, respectively (both p < .001). Upon long-term acclimation, the PxP fitness improved significantly, especially that the survival rate of immature stages increased to approximately 60% in the 15th, 30th, and 45th generations. However, PxP feeding on pea exhibited poorer fitness with longer larval developmental time, shorter total life span, lighter pupa, and lower fecundity in different generations compared with PxP feeding on radish. PxP feeding on pea also showed a significantly lower intrinsic rate of increase (r), net reproduction rate (R 0), finite increase rate (λ), and longer mean generation time (T) than PxP feeding on radish in all generations tested. Significant positive correlations were observed between pupal weight and female fecundity in pea-fed populations, and between female longevity and female fecundity in pea-fed and radish-fed populations. Our findings suggest that P. xylostella adaptation to pea does not improve overall fitness compared with the original host radish, making pea a marginal host for P. xylostella.

17.
World J Clin Cases ; 9(19): 5197-5202, 2021 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-34307567

RESUMO

BACKGROUND: Peliosis hepatis (PH) is a rare benign lesion of vascular origin with a pathological characteristic of multiple blood-filled cavities in the liver parenchyma. It is commonly misdiagnosed due to its lack of specificity in clinical presentation and laboratory test results. Herein, a case of a patient with PH who was misdiagnosed with hepatic echinococcosis before operation to remove the lesions was analyzed, with an emphasis on the computed tomography and magnetic resonance imaging characteristics of PH. CASE SUMMARY: We outline the case of a 40-year-old Chinese female who was admitted with aggravated abdominal pain with fever for 1 wk. Ultrasound examination at the local hospital indicated hepatic echinococcosis. However, discordance between imaging diagnosis, clinical history and laboratory examinations in our hospital. Subsequently, the patient was pathologically confirmed as having PH-like changes, which recurred 1 year after operation removal of the lesion. CONCLUSION: Our objective is to highlight the imaging diagnostic value of PH.

18.
Se Pu ; 39(8): 889-895, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34212589

RESUMO

Domoic acid (DA) can poison or even be fatal to marine mammals, and poses a potential risk to human health via transmission through the food chain. The level of DA in seawater will affect the safety of seafood. Therefore, a powerful method for the detection of DA in seawater, especially in the coastal mariculture zone, is needed. In order to identify different concentration levels of DA in real seawater, in this study, a method was established for the determination of trace DA in seawater by SPE-LC-MS/MS. First, the LC-MS/MS instrument and sample pretreatment conditions were optimized. Subsequently, DA was separated on a 5 TC-C18 (2) analytical column (150 mm×4.6 mm, 5 µm), and multiple reaction monitoring (MRM) was conducted in the positive electrospray ionization mode. For off-line SPE, the HLB cartridge could enrich DA in seawater. The best enrichment of DA was obtained after adding 0.32 mL formic acid to an 80.0 mL seawater sample. Four on-line SPE columns from Agilent, namely, 5 TC-C18(2) (12.5 mm×4.6 mm, 5 µm), Zorbax Eclipse Plus-C18 (12.5 mm×2.1 mm, 5 µm), Zorbax Eclipse XDB-C8 (12.5 mm×2.1 mm, 5 µm), and PLRP-S (12.5 mm×2.1 mm, 15-20 µm), were tested to determine their suitability to trap DA from seawater samples. The 5 TC-C18 (2) column offered the best retention ability and good peak shape of DA, and was selected as the on-line SPE column. Validation was then performed to assess the sensitivity, linearity, matrix effects (MEs), recoveries, and precisions of the proposed method. After simple treatment of the seawater samples by filtration and acidification, 0.6 mL of the seawater sample was injected directly for on-line SPE-LC-MS/MS. The linearity was good, and ranged from 10.0 to 500.0 ng/L (correlation coefficient R2=0.9992). The limit of detection (LOD) and limit of quantification (LOQ) of DA were 4.0 and 10.0 ng/L, respectively, with good recovery (≥81.0%) and precision (RSDs≤4.2%) at three spiked levels in the blank seawater samples. After the DA in the 80.0 mL seawater sample was enriched by off-line SPE, a 0.6 mL sample was injected for on-line SPE-LC-MS/MS. The DA in the spiked blank seawater sample showed a good linear relationship in the range of 0.3-50.0 ng/L (R2=0.9990). The LOD and LOQ were 0.1 and 0.3 ng/L, respectively. The recoveries of DA at low, medium, and high spiked levels in the blank seawater samples were all ≥69.2%, and the RSDs were ≤4.4%. The MEs of DA with both methods were 18.3% and 13.7%, respectively, indicating that the ME was mild enough to be negligible. In summary, the proposed method is simple, sensitive, robust, and powerful for the detection of DA in inshore and offshore seawater.


Assuntos
Ácido Caínico/análogos & derivados , Água do Mar/química , Cromatografia Líquida , Ácido Caínico/análise , Extração em Fase Sólida , Espectrometria de Massas em Tandem
19.
Environ Sci Pollut Res Int ; 28(46): 65188-65199, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34227011

RESUMO

It is essential for the sustainable development of agriculture to enhance nitrogen use efficiency (NUE) of crop plants by increasing yield and reducing nitrogen (N) losses. Biochar-based fertilizer (BF) has received increasing attention because of its full play to the advantages of chemical compounds with sufficient N and less N loss risk with good adsorption characteristics, but this potential was seldom reported for open-field vegetable crops, NUE of which were significantly lower than cereal crops. A field trial was conducted to investigate the efficacy of BF on NUE in vegetable cropping system by comparison with chemical fertilizer (CF) and partial substitution of organic fertilizers to chemical fertilizers (COF). The yield, plant N uptake, residual soil mineral N, and N losses via leaching and ammonia volatilization from an open vegetable (water spinach, Ipomoea aquatica L.) field were analyzed. The results indicated that BF treatment had significantly higher yield, plant N uptake, and NUE (agronomic efficiency and recovery efficiency as the NUE indicators), compared with those of CF and COF treatments. N losses via leaching were respectively accounted for 53.30%, 37.74%, and 33.39%; and N losses via ammonia volatilization were respectively accounting to 1.13%, 0.78%, and 1.54% of N fertilizer applied (at a rate of 200 kg N/ha) in CF, COF, and BF treatments. Despite the increasing ammonia volatilization due to the alkalinity of biochar, BF treatment significantly enhance NUE by increasing N uptake by water spinach and minimizing N losses via leaching. This study suggested that BF could serve as a promising slow-release N fertilizer for sustainable N management in field vegetable production and provided critical information for the development and dissemination of BF management guidelines.

20.
J Vasc Res ; 58(5): 330-342, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34247157

RESUMO

OBJECTIVE: This study investigates the synergistic effects of Gleevec (imatinib) and rapamycin on the proliferative and angiogenic properties of mouse bone marrow-derived endothelial progenitor cells (EPCs). MATERIALS AND METHODS: EPCs were isolated from mouse bone marrow and treated with different concentrations of Gleevec or rapamycin individually or in combination. The cell viability and proliferation were examined using the MTT assay. An analysis of cell cycle and apoptosis was performed using flow cytometry. Formation of capillary-like tubes was examined in vitro, and the protein expression of cell differentiation markers was determined using Western blot analysis. RESULTS: Gleevec significantly reduced cell viability, cell proliferation, and induced cell apoptosis in EPCs. Rapamycin had similar effects on EPCs, but it did not induce cell apoptosis. The combination of Gleevec and rapamycin reduced the cell proliferation but increased cell apoptosis. Although rapamycin had no demonstratable effect on tube formation, the combined therapy of Gleevec and rapamycin significantly reduced tube formation when compared with Gleevec alone. Mechanistically, Gleevec, but not rapamycin, induced a significant elevation in caspase-3 activity in EPCs, and it attenuated the expression of the endothelial protein marker platelet-derived growth factor receptor α. Functionally, rapamycin, but not Gleevec, significantly enhanced the expression of endothelial differentiation marker proteins, while attenuating the expression of mammalian target of rapamycin signaling-related proteins. CONCLUSIONS: Gleevec and rapamycin synergistically suppress cell proliferation and tube formation of EPCs by inducing cell apoptosis and endothelial differentiation. Mechanistically, it is likely that rapamycin enhances the proapoptotic and antiangiogenic effects of Gleevec by promoting the endothelial differentiation of EPCs. Given that EPCs are involved in the pathogenesis of some cardiovascular diseases and critical to angiogenesis, pharmacological inhibition of EPC proliferation by combined Gleevec and rapamycin therapy may be a promising approach for suppressing cardiovascular disease pathologies associated with angiogenesis.

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